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LT2948774T - Dnr modifikacijų nustatymo būdas, naudojant vieną molekulę - Google Patents

Dnr modifikacijų nustatymo būdas, naudojant vieną molekulę

Info

Publication number
LT2948774T
LT2948774T LTEP14701960.8T LT14701960T LT2948774T LT 2948774 T LT2948774 T LT 2948774T LT 14701960 T LT14701960 T LT 14701960T LT 2948774 T LT2948774 T LT 2948774T
Authority
LT
Lithuania
Prior art keywords
detection
single molecule
dna modifications
molecule manipulation
manipulation
Prior art date
Application number
LTEP14701960.8T
Other languages
English (en)
Inventor
David Bensimon
Vincent Croquette
Harold GOUET
Jean-Francois Allemand
Fang-Yuan Ding
Original Assignee
Centre National De La Recherche Scientifique (Cnrs)
Ecole Normale Supérieure
Sorbonne Université
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National De La Recherche Scientifique (Cnrs), Ecole Normale Supérieure, Sorbonne Université filed Critical Centre National De La Recherche Scientifique (Cnrs)
Publication of LT2948774T publication Critical patent/LT2948774T/lt

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/557Immunoassay; Biospecific binding assay; Materials therefor using kinetic measurement, i.e. time rate of progress of an antigen-antibody interaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/154Methylation markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
LTEP14701960.8T 2013-01-22 2014-01-22 Dnr modifikacijų nustatymo būdas, naudojant vieną molekulę LT2948774T (lt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP13305074 2013-01-22
PCT/EP2014/051272 WO2014114687A1 (en) 2013-01-22 2014-01-22 Process for detection of dna modifications and protein binding by single molecule manipulation

Publications (1)

Publication Number Publication Date
LT2948774T true LT2948774T (lt) 2018-10-10

Family

ID=47683668

Family Applications (1)

Application Number Title Priority Date Filing Date
LTEP14701960.8T LT2948774T (lt) 2013-01-22 2014-01-22 Dnr modifikacijų nustatymo būdas, naudojant vieną molekulę

Country Status (16)

Country Link
US (1) US9915655B2 (lt)
EP (1) EP2948774B1 (lt)
JP (1) JP6461008B2 (lt)
KR (1) KR102022427B1 (lt)
CN (2) CN108588208B (lt)
AU (1) AU2014209942B2 (lt)
BR (1) BR112015017354A2 (lt)
CA (1) CA2898151C (lt)
DK (1) DK2948774T3 (lt)
ES (1) ES2689676T3 (lt)
HK (1) HK1215602A1 (lt)
IL (1) IL239926B (lt)
LT (1) LT2948774T (lt)
RU (1) RU2678996C2 (lt)
SG (1) SG11201505618XA (lt)
WO (1) WO2014114687A1 (lt)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010037001A2 (en) 2008-09-26 2010-04-01 Immune Disease Institute, Inc. Selective oxidation of 5-methylcytosine by tet-family proteins
ES2872073T3 (es) 2011-12-13 2021-11-02 Univ Oslo Hf Procedimientos y kits de detección de estado de metilación
ES2669512T3 (es) 2012-11-30 2018-05-28 Cambridge Epigenetix Limited Agente oxidante para nucleótidos modificados
CN107075505A (zh) * 2014-09-29 2017-08-18 富士瑞必欧株式会社 含有修饰核酸碱基的靶核酸的测定方法及试剂盒
EP3090803B1 (en) 2015-05-07 2019-08-07 Paris Sciences et Lettres - Quartier Latin Improved device for the analysis of nucleic acid molecules
EP3292217B1 (en) 2015-05-07 2019-07-10 Paris Sciences et Lettres - Quartier Latin Formation of hairpins in situ using force-induced strand invasion
US11459573B2 (en) 2015-09-30 2022-10-04 Trustees Of Boston University Deadman and passcode microbial kill switches
KR101928659B1 (ko) 2017-06-19 2018-12-13 서울대학교병원 Dna 변형 유도 단백질 복합체 및 이를 암호화하는 유전자를 포함하는 재조합 벡터
WO2019030306A1 (en) 2017-08-08 2019-02-14 Depixus ISOLATION AND IN VITRO ENRICHMENT OF NUCLEIC ACIDS USING SITE-SPECIFIC NUCLEASES
CN110470844A (zh) * 2018-05-09 2019-11-19 中国科学院物理研究所 基于解旋酶检测dna与蛋白质相互作用的方法
AU2019380672A1 (en) 2018-11-16 2021-05-27 Depixus Optimization of In Vitro Isolation Of Nucleic Acids Using Site-Specific Nucleases
JP7405485B2 (ja) 2018-12-12 2023-12-26 ドピクサス 部位特異的核酸を用いた核酸濃縮と続いての捕捉方法
CN110396535B (zh) * 2019-05-08 2022-12-23 南开大学 单分子技术检测cgi序列甲基化调控cxxc结构域位点特异性结合的方法
CN111269911B (zh) * 2020-01-23 2023-05-02 南开大学 包括CpG位点的发夹结构及测量CpG毗邻序列影响蛋白解离时间常数的单分子力学方法
MX2023001142A (es) 2020-07-30 2023-05-25 Cambridge Epigenetix Ltd Composiciones y metodos para analisis de acidos nucleicos.
EP4160199B1 (en) 2021-10-04 2025-02-12 Depixus Apparatus for biomolecule analysis with a well and a cavity below the well
CN115753510A (zh) * 2021-11-23 2023-03-07 北京航空航天大学 Dna张力传感器、单细胞力学分析装置及使用方法
CN119563035A (zh) * 2022-04-01 2025-03-04 德皮克萨斯股份有限公司 筛选化合物的方法
EP4481057A1 (en) 2023-06-20 2024-12-25 Depixus Sample preparation methods using modified bases for rapid identification and sequencing

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE48140T1 (de) 1981-04-17 1989-12-15 Univ Yale Modifizierte nukleotide und verfahren zu ihrer herstellung und anwendung.
IL73577A (en) 1983-12-12 1989-10-31 Miles Lab Method and reagent system for detecting dna or rna sequences in a test medium containing single stranded dna or rna using antibodies to intercalation complexes with double stranded nucleic acid
CA1223222A (en) 1984-02-22 1987-06-23 Nanibhushan Dattagupta Immobilized nucleic acid-containing probes
AU1664092A (en) 1991-03-21 1992-10-21 Eastman Kodak Company Element and method for nucleic acid amplification and detection using adhered probes
US7052650B2 (en) 1997-03-28 2006-05-30 Center National De La Recherche Scientifique (Cnrs) Apparatus and method for the manipulation and testing of molecules, and in particular of DNA
US7244391B2 (en) 1997-03-28 2007-07-17 Centre National De La Recherche Scientifique (Cnrs) Apparatus and method for the manipulation and testing of molecules, and in particular of DNA
US6238869B1 (en) * 1997-12-19 2001-05-29 High Throughput Genomics, Inc. High throughput assay system
EP1088101A4 (en) * 1999-03-30 2004-10-20 Nanogen Inc DIFFERENTIATION OF SINGLE NUCLEOTIDE POLYMORPHISMS BY ELECTRONIC DOT BLOT TRIES ON SEMICONDUCTOR MICROCHIPS
JP5774805B2 (ja) * 2004-11-29 2015-09-09 セクエノム,インコーポレイティド メチル化dnaを検出する方法、及びキット
CN100424174C (zh) * 2005-09-01 2008-10-08 上海交通大学 原子力显微镜诱导单分子dna定位突变方法
EP2053379A1 (en) * 2007-10-24 2009-04-29 Nambition GmbH Method and apparatus for analysis of force traces
EP2390351A1 (en) 2010-05-27 2011-11-30 Centre National de la Recherche Scientifique (CNRS) Method of DNA sequencing by hybridisation
EP2390350A1 (en) * 2010-05-27 2011-11-30 Centre National de la Recherche Scientifique (CNRS) Method of DNA sequencing by polymerisation

Also Published As

Publication number Publication date
IL239926B (en) 2018-08-30
SG11201505618XA (en) 2015-08-28
WO2014114687A1 (en) 2014-07-31
ES2689676T3 (es) 2018-11-15
IL239926A0 (en) 2015-08-31
DK2948774T3 (en) 2018-09-24
EP2948774A1 (en) 2015-12-02
KR20160003629A (ko) 2016-01-11
CN105122063A (zh) 2015-12-02
AU2014209942B2 (en) 2019-01-31
JP6461008B2 (ja) 2019-01-30
RU2015132798A (ru) 2017-03-02
US9915655B2 (en) 2018-03-13
AU2014209942A1 (en) 2015-08-27
RU2678996C2 (ru) 2019-02-05
EP2948774B1 (en) 2018-07-04
US20150362488A1 (en) 2015-12-17
CA2898151C (en) 2021-05-25
CA2898151A1 (en) 2014-07-31
HK1215602A1 (zh) 2016-09-02
JP2016507744A (ja) 2016-03-10
CN108588208A (zh) 2018-09-28
BR112015017354A2 (pt) 2017-11-21
KR102022427B1 (ko) 2019-09-18
CN108588208B (zh) 2022-05-24

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