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LT2009032A - Conversion of alpha-hydroxyalkylated residues in biomolecules using methyltransferases - Google Patents

Conversion of alpha-hydroxyalkylated residues in biomolecules using methyltransferases

Info

Publication number
LT2009032A
LT2009032A LT2009032A LT2009032A LT2009032A LT 2009032 A LT2009032 A LT 2009032A LT 2009032 A LT2009032 A LT 2009032A LT 2009032 A LT2009032 A LT 2009032A LT 2009032 A LT2009032 A LT 2009032A
Authority
LT
Lithuania
Prior art keywords
alpha
targeted
conversion
biomolecules
methyltransferases
Prior art date
Application number
LT2009032A
Other languages
Lithuanian (lt)
Other versions
LT5706B (en
Inventor
Saulius KLIMA�AUSKAS
Zita LIUTKEVI�I�T�
Edita Kriukien�
Original Assignee
Biotechnologijos Institutas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to LT2009023A priority Critical patent/LT5708B/en
Application filed by Biotechnologijos Institutas filed Critical Biotechnologijos Institutas
Priority to LT2009032A priority patent/LT5706B/en
Priority to PCT/EP2010/054436 priority patent/WO2010115846A1/en
Priority to US13/262,738 priority patent/US8889352B2/en
Priority to EP10714602.9A priority patent/EP2414528B1/en
Priority to PCT/EP2010/054437 priority patent/WO2010115847A1/en
Priority to EP10712086.7A priority patent/EP2414527B1/en
Priority to US13/262,340 priority patent/US8822146B2/en
Publication of LT2009032A publication Critical patent/LT2009032A/en
Publication of LT5706B publication Critical patent/LT5706B/en
Priority to US14/542,770 priority patent/US9505797B2/en
Priority to US15/293,232 priority patent/US20170067093A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/91005—Transferases (2.) transferring one-carbon groups (2.1)
    • G01N2333/91011—Methyltransferases (general) (2.1.1.)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to targeted conversion of alpha-hydroxyalkylatedresidues in biomolecules in the presence of a directing methyltransferase, namely, to targeted removal of the alpha-hydoxyalkyl moieties to give unmodified residues, or targeted derivatization of the alpha-hydroxyalkyl groups by covalent coupling of non-cofactor compounds represented by formula HQ-LX, wherein X represents a functional group or a reporter group attached via a linker moiety L, and QH is selected from HS-, HSe-, HO- H2N-, HN3 or HCN in the presence of a directing methyltransferase. Further development of the method of targeted conversion comprises methods for targeted labeling a biomolecule and methos for detecting hydroxymethylated target sites in a biomolecule according to the present invention.á
LT2009032A 2009-04-02 2009-05-08 Conversion of alpha-hydroxyalkylated residues in biomolecules using methyltransferases LT5706B (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
LT2009023A LT5708B (en) 2009-04-02 2009-04-02 Derivatization of biomolecules by covalent coupling of non-cofactor compounds using methyltransferases
LT2009032A LT5706B (en) 2009-04-02 2009-05-08 Conversion of alpha-hydroxyalkylated residues in biomolecules using methyltransferases
PCT/EP2010/054437 WO2010115847A1 (en) 2009-04-02 2010-04-01 Conversion of alpha-hydroxyalkylated residues in biomolecules using methyltransferases
US13/262,738 US8889352B2 (en) 2009-04-02 2010-04-01 Conversion of alpha-hydroxyalkylated residues in biomolecules using methyltransferases
EP10714602.9A EP2414528B1 (en) 2009-04-02 2010-04-01 Derivatization of biomolecules by covalent coupling of non-cofactor compounds using methyltransferases
PCT/EP2010/054436 WO2010115846A1 (en) 2009-04-02 2010-04-01 Derivatization of biomolecules by covalent coupling of non-cofactor compounds using methyltransferases
EP10712086.7A EP2414527B1 (en) 2009-04-02 2010-04-01 Conversion of alpha-hydroxyalkylated residues in biomolecules using methyltransferases
US13/262,340 US8822146B2 (en) 2009-04-02 2010-04-01 Derivatization of biomolecules by covalent coupling of non-cofactor compounds using methyltransferases
US14/542,770 US9505797B2 (en) 2009-04-02 2014-11-17 Conversion of alpha-hydroxyalkylated residues in biomolecules using methyltransferases
US15/293,232 US20170067093A1 (en) 2009-04-02 2016-10-13 Conversion of alpha-hydroxyalkylated residues in biomolecules using methyltransferases

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
LT2009023A LT5708B (en) 2009-04-02 2009-04-02 Derivatization of biomolecules by covalent coupling of non-cofactor compounds using methyltransferases
LT2009032A LT5706B (en) 2009-04-02 2009-05-08 Conversion of alpha-hydroxyalkylated residues in biomolecules using methyltransferases

Publications (2)

Publication Number Publication Date
LT2009032A true LT2009032A (en) 2010-11-25
LT5706B LT5706B (en) 2011-01-25

Family

ID=42300437

Family Applications (2)

Application Number Title Priority Date Filing Date
LT2009023A LT5708B (en) 2009-04-02 2009-04-02 Derivatization of biomolecules by covalent coupling of non-cofactor compounds using methyltransferases
LT2009032A LT5706B (en) 2009-04-02 2009-05-08 Conversion of alpha-hydroxyalkylated residues in biomolecules using methyltransferases

Family Applications Before (1)

Application Number Title Priority Date Filing Date
LT2009023A LT5708B (en) 2009-04-02 2009-04-02 Derivatization of biomolecules by covalent coupling of non-cofactor compounds using methyltransferases

Country Status (4)

Country Link
US (4) US8822146B2 (en)
EP (2) EP2414528B1 (en)
LT (2) LT5708B (en)
WO (2) WO2010115847A1 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9115386B2 (en) 2008-09-26 2015-08-25 Children's Medical Center Corporation Selective oxidation of 5-methylcytosine by TET-family proteins
LT5708B (en) 2009-04-02 2011-01-25 Biotechnologijos Inst Derivatization of biomolecules by covalent coupling of non-cofactor compounds using methyltransferases
US20120064521A1 (en) * 2010-09-09 2012-03-15 James Yen Detection of dna hydroxymethylation
AT511856A1 (en) * 2011-09-01 2013-03-15 Univ Graz Tech MEDIUM AND METHOD OF ALKYLATION
GB201119903D0 (en) 2011-11-17 2011-12-28 Univ Vilnius Nucleic acid production and sequence analysis
GB201119904D0 (en) * 2011-11-17 2011-12-28 Univ Vilnius Analysis of methylation sites
EP3904533B1 (en) 2011-12-13 2025-02-26 Oslo Universitetssykehus HF Method for detection of hydroxymethylation status
ES2669512T3 (en) 2012-11-30 2018-05-28 Cambridge Epigenetix Limited Oxidizing agent for modified nucleotides
WO2016164363A1 (en) * 2015-04-06 2016-10-13 The Regents Of The University Of California Methods for determing base locations in a polynucleotide
WO2017059245A2 (en) 2015-09-30 2017-04-06 Trustees Of Boston University Deadman and passcode microbial kill switches
WO2020013712A1 (en) * 2018-07-09 2020-01-16 Victoria Link Limited Inhibitors of dnmt1 as anticancer therapeutic agents
GB2580963C (en) * 2019-02-01 2025-09-03 Hemispherian As Cancer therapies
CN110470754B (en) * 2019-07-31 2021-05-04 武汉大学 A kind of high-performance liquid chromatography method for the determination of cordycepin content in Cordyceps
JP2023535636A (en) 2020-07-30 2023-08-18 ケンブリッジ エピジェネティックス リミテッド Compositions and methods for nucleic acid analysis
CN112730554B (en) * 2020-12-09 2022-09-09 山东师范大学 Electrode, preparation of biosensor containing electrode and application of electrode in detection of methyltransferase

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI981790A0 (en) 1998-07-14 1998-08-20 Markku Rosnell Tools for treating a reef
DK1102781T3 (en) 1998-07-29 2004-04-05 Max Planck Gesellschaft Novel cofactors for methyltransferases
EP1712557A1 (en) 2005-04-14 2006-10-18 RWTH Aachen New s-adenosyl-L-methionine analogues with extended activated groups for transfer by methyltransferases
US7465544B2 (en) 2006-01-11 2008-12-16 Wisconsin Alumni Research Foundation Synthetic cofactor analogs of S-adenosylmethionine as ligatable probes of biological methylation and methods for their use
LT5708B (en) * 2009-04-02 2011-01-25 Biotechnologijos Inst Derivatization of biomolecules by covalent coupling of non-cofactor compounds using methyltransferases

Also Published As

Publication number Publication date
US8889352B2 (en) 2014-11-18
LT5706B (en) 2011-01-25
US20120094280A1 (en) 2012-04-19
US20120088238A1 (en) 2012-04-12
EP2414528B1 (en) 2014-05-07
US20150093752A1 (en) 2015-04-02
WO2010115846A1 (en) 2010-10-14
EP2414527B1 (en) 2015-05-20
EP2414528A1 (en) 2012-02-08
EP2414527A1 (en) 2012-02-08
LT5708B (en) 2011-01-25
US20170067093A1 (en) 2017-03-09
WO2010115847A1 (en) 2010-10-14
US8822146B2 (en) 2014-09-02
LT2009023A (en) 2010-10-25
US9505797B2 (en) 2016-11-29

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