KR20230060104A - Pet feed additive composition for improving liver function or immunity effect comprising Mealworm oil as effective component and uses thereof - Google Patents

Abstract

The present invention relates to a feed additive for companion animals having an effect of improving liver function or enhancing immunity, containing wheatworm oil as an active ingredient, and its use. The feed additive for companion animals of the present invention improves liver function and enhances immunity of companion animals. , It is effective in improving hair and tear marks, so it will be very useful in pet food related industries.

Description

[0001] Pet feed additive composition for improving liver function or immunity effect comprising mealworm oil as effective component and uses thereof}

The present invention relates to a feed additive for companion animals having an effect of improving liver function or enhancing immunity, containing wheatworm oil as an active ingredient, and its use.

Although companion animals grow up being protected as members of the family, they are frequently exposed to polluted natural environments, processed foods, and stress, such as air or water pollution that humans experience. For this reason, various diseases such as skin diseases, respiratory diseases, digestive disorders, etc., which are common in humans, are also developing in companion animals. In addition, since companion dogs live indoors, they are concerned about obesity due to low exercise, and there is a high possibility of secondary diseases due to this, so medicines and feeds using natural products exclusively for companion animals that can lead a healthy life by increasing immunity Material discovery is a very important task.

Edible insects are foods with high nutritional value due to their high protein and unsaturated fatty acid content, high content of essential amino acids and minerals such as iron and phosphorus, and high amounts of nutrients such as vitamins. Edible insects include brown mealworm larvae, twin crickets, rice locusts, white river sleep silkworms, silkworm pupae, white-spotted flower larvae, and beetle larvae. Edible insects are produced as circular dried products, but most of them are produced in powder form due to visual aversion. Recently, new products have been launched so that consumers can consume them in various forms. When using the extract, beneficial ingredients of edible insects can be consumed without disgust, and the unique taste and aroma can be maintained. Although insects are not yet widely used as food ingredients in Korea, it is expected that efforts to utilize insects as food materials will gradually increase due to problems such as food shortage in the future. In fact, insects contain many useful nutrients and are expected to be widely used when developed as feed ingredients.

On the other hand, Korean Patent Publication No. 2021-0096962 discloses a 'companion animal feed composition having an anti-inflammatory effect of brown mealworm hydrolyzate', and Korean Patent No. 2075539 discloses 'companion animal containing mackerel by-product and vitamin tree fruit'. 'Animal feed and its manufacturing method' is disclosed, but there is no description of a feed additive for companion animals containing the mealworm oil of the present invention as an active ingredient and having an effect of improving liver function or enhancing immunity and its use.

The present invention was derived from the above needs, and the present inventors found that the wheatworm oil treatment group has better liver function and immunity enhancement effects of companion animals than the wheatworm oil non-treatment group and the mealworm extract or mealworm hydrolysate treatment group, , The present invention was completed by confirming that there is an effect of improving hair shine and improving tear marks by inhibiting hypersecretion of tears.

In order to solve the above problems, the present invention provides a feed additive for companion animals having an effect of improving liver function or enhancing immunity, containing wheatworm oil as an active ingredient.

In addition, the present invention provides a companion animal food containing the feed additive.

In addition, the present invention provides a feed composition for companion animals containing the feed additive.

In addition, the present invention provides a method for breeding companion animals by feeding the feed composition.

The feed additive for companion animals containing the mealworm oil of the present invention is excellent in enhancing the liver function and immunity of companion animals, suppresses excessive secretion of tears in dogs and is effective in improving coat quality, so it is suitable for use in pet food-related industries. It will be very useful.

1 is a result of confirming the fatty acid composition in wheat worm oil prepared by milking wheat worms roasted at 200 ° C.
2 is a result of confirming the fatty acid composition in wheat worm oil prepared by milking wheat worms roasted at 250 ° C.
3 is a result of confirming the acid value and peroxide value of wheat worm oil prepared by cold pressing at 49 ° C.
4 is a result of confirming the acid value and peroxide value of wheat worm oil prepared by a hot pressing method at 200 ° C.
5 is a result showing the contents of omega 3, omega 6 and omega 9 in wheat worm oil according to pressing temperature.
6 is a photograph confirming the effect of improving tear marks by suppressing hypersecretion of tears for companion animals fed feed containing mealworm oil.
7 is a photograph confirming the hair improvement (hair gloss enhancement) effect for companion animals fed feed containing mealworm oil.

In order to achieve the object of the present invention, the present invention provides a feed additive for companion animals having an effect of improving liver function or enhancing immunity, containing wheatworm oil as an active ingredient.

The feed additive of the present invention contains wheatworm oil as an active ingredient, which is excellent in reducing ALT (alanine aminotransferase) and AST (aspartate aminotransferase) and increasing blood IgG (immunoglobulin G) concentration, thereby improving liver function and It has an immunity boosting effect. In addition, the feed additive of the present invention has an effect of improving tear marks by improving the hair of companion animals or suppressing hypersecretion of tears.

In the feed additive of the present invention, the wheat worm oil may be prepared by roasting 330 g of wheat worms at 200 ° C. or 250 ° C. at 60 rpm 3 to 5 times and then milking them at 50 Mpa for 10 minutes. Not limited.

In the present invention, the term "companion animal" refers to dogs, cats, rabbits, ferrets, guinea pigs, hamsters, etc. raised for the purpose of companionship, and the scope of the animals includes without limitation as long as they live with humans. In the present invention, the companion animal may be specifically a dog or cat, and more specifically may be a dog, but is not limited thereto.

The present invention also provides a companion animal food containing the feed additive.

The companion animal food of the present invention may further include sub-additives commonly included in companion animal foods in the art, specifically, a vitamin mixture including vitamin C and vitamin D, and containing iron and copper. Mineral mixtures, gelling agents such as agar, taurine, and the like may be further included. The content of the sub-additive may be determined by an amount generally formulated in the art, but is not limited thereto. However, the companion animal food of the present invention is characterized in that it does not contain preservatives, coloring agents, sweeteners, and seasonings.

The present invention also provides a feed composition for companion animals containing the feed additive.

In the present invention, the term "feed" means a substance that supplies organic or inorganic nutrients necessary for maintaining and breeding the life of an individual. The feed may include nutrients such as energy, protein, lipids, vitamins, and minerals required by an individual consuming the feed, but is not particularly limited thereto. The object refers to a breeding subject, and is included without limitation as long as it is a living organism capable of ingesting the feed of the present invention, and includes companion animals and livestock for the purpose of the present invention.

The feed composition of the present invention can be produced in various forms such as dry feed, semi-moist feed, and wet feed according to a method known in the art, for example, powder, granule, pill, pellet, jelly , canned feed, biscuits, croquettes, nuggets, flakes, snacks, etc., but may be made in any one form, but is not limited thereto.

The feed composition of the present invention may include one or more carriers, excipients, or diluents, examples of which include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, and starch. , acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. . In addition, fillers, anti-coagulants, lubricants, wetting agents, flavoring agents, emulsifiers and preservatives may be further included.

In addition, the feed composition of the present invention may include ingredients commonly added to feed such as carbohydrates, proteins, fats, minerals, vitamins, minerals, and water. There is no particular restriction on the type of each of these components, and all commonly used ones in the art can be used.

The feed composition of the present invention preferably contains 10% of wheat worm oil, 10% of wheat worm meal left over from the production of wheat worm oil, 30% of brown rice, 10% of brown rice, 8% of rice flour, 7% of sweet potato, 5% of potato, and 1 carrot. %, cabbage 1%, sweet pumpkin 1%, shiitake mushroom 1%, broccoli 1%, lotus root 1%, spinach 1%, chickpea 2%, hydrolyzed soybean protein 3%, various minerals (calcium phosphate, dicalcium phosphate) It may be prepared by mixing 2%, vitamins (A, B12, C, D, E) 2% and natural tocopherol 4%, but is not particularly limited thereto.

In the case of conventional pet food, fish oil, chicken oil, etc. are applied to the surface of the feed to increase the fat content and improve palatability, but in the feed of the present invention, the content of low-grade fat is reduced by adding wheatworm oil without adding the above oils. It has the effect of improving the digestive function of companion animals.

The feed composition according to the present invention can improve nutritional status by supplying various external natural nutrients to companion animals, strengthen basic physical strength to quickly build an initial immune system, and improve immunity by enhancing disease resistance. Here, immunity enhancement means having the ability to stimulate specific immune functions important to the health of companion animals, or to modulate other immune functions that can potentially be involved in immune disorders such as inflammation, allergy, etc., and stabilization of the intestinal flora. , including reduction of mortality, and both avoidance and inhibition of inflammation and diseases such as bacterial and viral infections.

According to one embodiment of the present invention, as a result of feeding the pet with the mealworm oil-containing feed, a normal feed to which nothing is added is fed, mealworm hot water extract, mealworm ethanol extract, mealworm hydrolysate or mealworm powder. It has been confirmed that immunity is strengthened compared to companion animals fed feed containing . Since it can be mixed and fed with the existing general pet food, it is easy for companion animals to consume and the application range is wide.

The present invention also provides a method for breeding companion animals by feeding the feed composition. Feeding of companion animals may be divided into 1 to 5 times a day, but is not limited thereto.

Hereinafter, the present invention will be described in detail through examples. It should be noted that the specific examples and examples below include some embodiments of the invention, but not all embodiments. It is obvious that the content of the invention disclosed by this specification is not limited to the specific embodiments described herein, and includes that a person skilled in the art can implement it in various ways in the technical field to which the present invention belongs. Therefore, it should be understood that the content of the invention disclosed in this specification is not limited to the specific embodiments described herein, and modifications and other embodiments thereof are also included within the scope of the claims.

Preparation Example 1. Mealworm Oil

After roasting 330 g of mealworms (brown mealworm larvae) at 200℃ or 250℃ at 60 rpm 3 to 5 times, milking by low-temperature pressing method at 50 Mpa, 49℃ (120℉) for 10 minutes, was manufactured.

Comparative Example 1. Mealworm Hot Water Extract Preparation

Mealworm dry powder and water were mixed, extracted at 90 ° C. for 24 hours and filtered to prepare a mealworm hot water extract, which was lyophilized to obtain a powder.

Comparative Example 2. Mealworm Ethanol Extract Preparation

Mealworm dry powder and ethanol were mixed, extracted at 80° C. for 6 hours, and filtered under reduced pressure to prepare an ethanol extract of wheatworm, which was lyophilized to obtain a powder.

Comparative Example 3. Mealworm hydrolysate preparation

After dissolving the lyophilized powder of the hot water extract of wheatworm in distilled water, 1% (w/w) of Neutrase was added thereto, followed by stirring at 65° C. for 2 hours. Then, neutrase was inactivated by heating at 105° C. for 20 minutes, centrifuged, and a supernatant was obtained to prepare a mealworm hydrolyzate, which was lyophilized to obtain a powder.

Example 1. Fatty Acid Analysis of Mealworm Oil

Suwon Women's University Food Analysis Research Center was commissioned to analyze the fatty acid composition of wheat worm oil prepared by milking the wheat worm roasted at 200 ° C (Fig. 1) or 250 ° C (Fig. 2).

As a result, mealworm oil contained lauric acid (C12:0), tridecanoic acid (C13:0), myristic acid (C14:0), and pentadecanoic acid (C15). :0), Palmitic acid (C16:0), Palmitoleic acid (C16:1), Margaric acid (C17:0), Stearic acid (C18:0) , Oleic acid (C15: 1, cis), linoleic acid (C18: 2, Cis/trans), arachidic acid (C20: 0), linolenic acid (C18: 3n-3), Fatty acid components of Gadoleic acid (C20:1), Heneicosanoic acid (C21:0), Eicosadienoic acid (C20:0) and Tricosanoic acid (C23:0) It was confirmed that wheat worm oil prepared by milking wheat worms roasted at 200 ° C additionally contained a trans oleic acid (C15: 1) component.

In particular, among the various fatty acids contained in mealworm oil, palmitic acid, oleic acid (Cis type) and linoleic acid are contained in large amounts (Table 1), and it is prepared by milking mealworms roasted at 200 ° C or 250 ° C rather than unprocessed mealworms. It was found that the contents of palmitic acid, oleic acid (Cis type) and linoleic acid were higher in the mealworm oil of the present invention.

In addition, Korea Pet Food Research Institute Co., Ltd. commissioned to

As a result, the acid value and peroxide value of wheatworm oil produced by cold pressing were 0.52 mg/g and 6.00 meq/kg, respectively (FIG. 3), and the acid value and peroxide value of wheatworm oil produced by hot pressing were 1.79 mg and 22.00, respectively. In meq/kg (FIG. 4), it was confirmed that the acid value and peroxide value were significantly reduced when wheat worm oil was produced by the cold pressing method compared to the wheat worm oil produced by the hot pressing method.

In addition, as a result of measuring the content of omega 3, omega 6 and omega 9 components in mealworm oil according to the pressing temperature of mealworms, the omega components began to be destroyed at about 50 ° C, and at 160 ° C, the omega content decreased to less than 50%. It was confirmed (FIG. 5). Through this, it was found that the low-temperature pressing (cold pressing) method can improve the quality and freshness by reducing the rancidity of wheat worm oil.

Example 2. Immunity enhancing effect of wheat worm oil

The prepared mealworm oil or mealworm extract was added to commercially available general dog food (Home Family 10kg, Daehan Feed Co., Ltd.) and mixed to prepare a feed, and the immunity enhancing effect thereof was confirmed. Specifically, 5-month-old puppies (species: Maltese, average weight: 0.5 to 1 kg) were divided into 5 each, and wheatworm oil untreated group (basic feed feeding; negative control group), wheatworm oil treated group (Preparation Example 1; experimental group) ), Mealworm hot water extract treatment group (Comparative Example 1), Mealworm ethanol extract treatment group (Comparative Example 2), Mealworm hydrolysate treatment group (Comparative Example 3), and Mealworm powder treatment group (Comparative Example 4). They were fed daily and allowed to drink water ad libitum. After 30 days from feeding, blood was collected from each group, and the concentration of IgG (immunoglobulin G), one of immunoglobulins, was measured by performing an immunoenzyme assay, ELISA.

As a result, as shown in Table 2 below, it was confirmed that the experimental group fed the feed containing wheatworm oil of the present invention significantly increased the blood IgG concentration compared to the negative control group and the control group. Therefore, through the above results, it was found that the feed composition containing wheatworm oil of the present invention has an effect of enhancing the immunity of companion animals by increasing antibodies, particularly IgG, in the blood.

immune boosting effect group extract IgG (mg/dl) negative control Untreated (basic feed) 614±023 experimental group Production Example 1 (mealworm oil) 1,310±141 control group Comparative Example 1 (Mealworm hot water extract) 1,012±117 Comparative Example 2 (mealworm ethanol extract) 981±665 Comparative Example 3 (mealworm hydrolyzate) 842±433 Comparative Example 4 (mealworm powder) 998±053

Example 3. Mealworm oil improves liver function

In order to verify the liver function improvement effect of wheatworm oil, HepG2 cells, a human liver cancer cell line used in experiments related to liver function, were used. HepG2 cells were dispensed in a 96-well plate at 1×10 ⁴ cells/well and cultured in an incubator at 37° C. under 5% CO ₂ conditions for 24 hours. Then, the sample was pretreated for 2 hours, treated with H ₂ O ₂ (3,000 μm), a hepatotoxic substance, and then incubated in an incubator at 37°C, 5% CO ₂ for 24 hours, Asan Pharmaceutical's GOT (AST), AST (aspartate aminotransferase) and ALT (alanine aminotransferase) levels were measured using a reagent kit for measuring GPT (ALT).

As a result, as shown in Table 3 below, it was confirmed that the increased AST and ALT by H ₂ O ₂ treatment were significantly reduced in the wheatworm oil treatment group of the present invention than in the negative control group and the control group.

Liver function improvement effect group extract AST(U/L) ALT(U/L) negative control Untreated (basic feed) 109.1 68.9 experimental group Production Example 1 (mealworm oil) 33.4 21.0 control group Comparative Example 1 (Mealworm hot water extract) 52.9 51.5 Comparative Example 2 (mealworm ethanol extract) 78.1 42.3 Comparative Example 3 (mealworm hydrolyzate) 69.0 45.0 Comparative Example 4 (mealworm powder) 59.6 48.1

Example 4. Mealworm oil inhibiting hypersecretion of tears

Tear marks are a common phenomenon in various dogs, and are colored due to 'porphyrin', an iron component contained in dog tears. Since tear disease in dogs is frequently caused by an allergic reaction to food, it should be replaced with low-allergy food or food that does not contain any coloring or preservatives.

In the present invention, feed containing mealworm oil was fed to puppies for 2 weeks, and the degree of tear secretion and tear stain removal was visually observed and evaluated as a clinical improvement index.

As a result, as shown in Table 4 below, it was confirmed that tear hypersecretion was relatively suppressed in the experimental group fed the feed containing the mealworm oil of the present invention compared to the negative control group and the control group. In addition, it was confirmed that tear marks were improved compared to the control group without mealworm oil treatment when the feed containing wheatworm oil of the present invention was fed to puppies (FIG. 6).

Tear hypersecretion inhibitory effect clinical symptoms
Whether or not there is improvement voice
control group Preparation Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 100% improvement compared to the first time (4 points) 75% improvement from the beginning (3 points) ● 50% improvement from the beginning (2 points) ● ● 25% improvement from the beginning (1 point) ● ● No improvement compared to initial (0 points) ●

Example 5. Hair improvement effect of mealworm oil

In order to evaluate the effect of mealworm oil on companion animal hair improvement, hair obtained after dog grooming was dyed and damaged, and then mealworm oil, mealworm hot water extract, mealworm ethanol extract, or mealworm hydrolyzate were applied to the dyed hair 10 times. After washing and drying, the gloss of the hair was measured using a goniophotometer.

As a result, as shown in Table 5 below, it was confirmed that hair gloss increased in the mealworm oil treatment group of the present invention compared to the control group (distilled water treatment) and the control group (mealworm hot water extract, mealworm ethanol extract, or mealworm hydrolysate treatment). did

hair shine evaluation control group Preparation Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 hair shine 26.1 62.7 45.1 51.2 49.8

In addition, as a result of visually observing the degree of gloss of the hair after feeding the feed without wheatworm oil and the feed with wheatworm oil added to the puppies for 2 weeks, the feed containing wheatworm oil of the present invention was fed to the companion animal. In this case, it was confirmed that the hair gloss was enhanced compared to the control group without mealworm oil treatment (FIG. 7).

Claims (5)

A feed additive for companion animals that contains wheatworm oil as an active ingredient and has an effect of improving liver function or enhancing immunity. The feed additive for companion animals according to claim 1, wherein the mealworm oil has an effect of improving hair or tear stains of companion animals, improving liver function or enhancing immunity. A food for companion animals comprising the feed additive of claim 1 or 2. A pet food composition comprising the feed additive of claim 1 or 2. A method of breeding companion animals by feeding the feed composition of claim 4.

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