KR20220037667A - Method for mass propagation of high quality seedlings of Myrica rubra - Google Patents

Abstract

The present invention relates to a method for mass propagation of high-quality seedlings of myrica rubra. According to the present invention, the rates of seed germination and long-term stable seedling growth are remarkably improved through dry heat pretreatment and gibberellin treatment of myrica rubra seeds under specific conditions. With the present invention, the rate of myrica rubra seed germination can be increased and root elongation can be improved at the same time. In addition, the rate of long-term stable growth of the germinated myrica rubra seedlings is remarkably improved. Accordingly, mass propagation of high-quality myrica rubra seedlings resistant to stresses such as high temperature, excessive density, and excessive fertilization can be performed. In addition, the high-quality myrica rubra seedlings of the present invention are healthy seedlings suitable for long-term growth and thus can be utilized for mass afforestation.

Description

{Method for mass propagation of high quality seedlings of Myrica rubra}

The present invention relates to a method for mass production of high-quality seedlings of bovine oak, and more particularly, through a dry heat pretreatment process under specific conditions and a gibberellin treatment process under specific conditions, the seed germination rate and long-term stable growth rate of seedlings are significantly improved. It relates to a method for mass production of excellent seedlings of old pine trees.

It belongs to the Sogwiaceae family and is a rare plant native to Jeju Island, and is known to be distributed in China, Taiwan, Japan, and the Philippines. It is used for edible, medicinal, and dyeing purposes, and is also used for syntanza, garden trees, street trees, park trees, and ornamental trees. The fruit is effective for diarrhea, digestion improvement, thirst quenching, stomatitis, and bruising. In recent years, cosmetics have been developed, and improved varieties are being cultivated, called sheep berry and red bayberry. In addition, it has been found that the ginseng tree is effective in hair loss, prevention of skin aging, and treatment of mites allergens (Registration Patent No. 10-0872454. Patent Publication No. 10-2008-0078753).

As such, the pine tree has a high conservation value as a rare plant, as well as a high utility value and ornamental value as a natural material. It was difficult to mass-produce high-quality seedlings because stable growth was not guaranteed.

Enhancement of plant seed germination rate and long-term stable growth rate of seedlings is very important in plant cultivation. As seed treatment to increase the germination rate of seeds, priming treatment, hormone treatment including gibberellin, and light treatment have been used (Registration Patent No. 10-1412052), but a method for simultaneously enhancing the long-term stable growth rate of seedlings has been developed. There is no bar. In particular, there has been no report so far on a technique for simultaneously enhancing the seed germination rate and long-term stable growth rate of seedlings in the case of cypress trees.

Therefore, there is an urgent need for the development of a method for mass-producing high-quality seedlings of calamus trees by simultaneously enhancing the seed germination rate and long-term stable growth rate of seedlings.

Accordingly, the present inventors have continuously studied to meet the needs in the prior art. As a result, when a dry heat pretreatment process under specific conditions and a gibberellin treatment process under specific conditions are combined, the seed germination rate and long-term The present invention was completed by confirming that the stable growth rate was remarkably improved and it was possible to mass-produce excellent seedlings of the cypress tree.

Accordingly, it is an object of the present invention to provide a method for mass production of excellent seedlings of spruce.

It is another object of the present invention to provide excellent seedlings of spruce produced by the above method.

In order to achieve the above object of the present invention, the present invention

i) sterilizing the seeds of the bovine oak;

ii) dry heat treatment of the sterilized seeds at 35-45° C. for 15-55 hours;

iii) immersing the dry heat treated seeds in an aqueous solution of gibberellin at a concentration of 500 to 1,500 ppm for 20 to 24 hours;

iv) sowing and germinating the sowing seeds from step iii); and

v) growing the germinated seedlings to obtain excellent seedlings of serrata.

Step i) Seed Sterilization

Sterilize the sagebrush seeds.

In the present invention, sterilization can be performed by immersing the seeds of the bovine serrata in water for 3 to 5 days, washing them, then treating them in sodium hypochlorite (NaOCl) solution for 5 to 10 minutes, and then washing with water. Preferably, after 5 days of immersing the seeds of bovine oak in running water, treatment with 1% sodium hypochlorite for 10 minutes, washing three times in running water, and then washing once with distilled water to sterilize the seeds of bovine tree.

According to this step, it is possible to remove microorganisms and germination inhibitors that can cause rot on the surface of the seeds without lowering the germination rate when sterilizing the seeds of bovine serrata. Germination can also be promoted by softening the tissue.

step ii) dry heat treatment

Sterilized seeds are treated with dry heat at 35-45°C for 15-55 hours.

In this step, 'dry heat treatment' means heat treatment using dry heat rather than immersion using hot water, and preferably, dry heat treatment using a dry heat machine or a dry oven, but is not limited thereto.

The dry heat treatment temperature is preferably maintained at a constant temperature in the range of 35 to 45 ° C. If the temperature is less than 35 ° C, the heat treatment effect may be insufficient, and if it exceeds 45 ° C, the seeds may be altered. Most preferably, dry heat treatment is performed at 40°C.

The dry heat treatment time is preferably 15 to 55 hours. If it is less than 15 hours, the heat treatment effect may be insufficient, and if it exceeds 55 hours, the seeds may deteriorate.

According to this step, the long-term stable growth rate of seedlings is remarkably improved without a decrease in the germination rate of the seedlings when dry-heat treatment of the seeds of the cypress seedlings is performed (FIGS. 7a, 7b, Table 2).

Step iii) Gibberellin treatment

The dry heat treated seeds are immersed in an aqueous gibberellin solution at a concentration of 500 to 1,500 ppm for 20 to 24 hours.

Gibberellin (GAs) is a plant hormone that regulates plant growth such as stem elongation, germination, dormancy, flowering and growth of flowers, and aging of leaves and fruits.

In this step, the concentration of gibberellin is preferably 500 to 1,500 ppm, and most preferably 1,000 ppm.

If the concentration of gibberellin is less than 500ppm, the germination rate may not be improved properly, and if it exceeds 1,500ppm, it may cause a germination inhibitory action.

The treatment time of the gibberellin is preferably 20 to 24 hours, and if the gibberellin treatment time is less than 20 hours, the gibberellin may not sufficiently penetrate the seeds, and if it exceeds 24 hours, the penetration of the gibberellin does not occur anymore and is not efficient.

When only the gibberellin treatment was performed without the dry heat treatment of step ii), the germination rate of the bovine oak seeds was improved, but there was a problem in that the long-term stable growth rate of the seedlings was lowered ( FIGS. 7a , 7b , Table 2).

After the dry heat treatment in step ii), when the gibberellin treatment is performed according to this step, the germination rate of bovine oak seeds can be further improved, and the long-term stable growth rate of seedlings can be maintained high ( FIGS. 7a, 7b, Table 2).

Step iv) germination

Seed from step iii) is sown and germinated.

Sowing at this stage is preferably done in horticultural top soil at a temperature of 12 ~ 27 ℃. If it is outside the above temperature range, germination may be inhibited.

For point sowing, the spacing is preferably 5 cm or more. If this range is not reached, germination and growth may be inhibited as the seedlings grow, such as tangled roots, overlapping leaves, or lack of nutrients.

If it is hot and humid and the water washing is weak, it is preferable to do not fertilize or to do it in a small amount.

Sowing is usually carried out under dark conditions by covering the soil to retain moisture of the seeds.

At this stage, it is preferable to perform germination under light-shielding conditions.

Step v) Growth

The germinated seedlings are grown to obtain excellent seedlings.

Growth at this stage can be carried out by growing for 10 to 24 months after sowing at a temperature of 0.5 to 43.5 ° C under light-shielding conditions.

If the growth temperature is less than 0.5 ℃, the growth of the seedlings of the cypress tree may not be made sufficiently, and if it is higher than 43.5 ℃, the growth of the nursery of the cypress tree may be inhibited due to the high temperature.

Preferably, the seedlings are transplanted into new soil at intervals of 15 to 25 cm and grown 6 to 8 months after sowing. In this case, it is preferable that the height of the seedlings be 2 cm or more, the diameter of the seedlings is 0.7 mm or more, and the number of leaves is 4 or more. If this number is not reached, the possibility of growth after transplantation is low.

According to the production method of the present invention, the germination rate at 210 days after sowing of the seeds of the pine tree is 78% or more, and the growth rate at 306 days after sowing is 66% or more.

According to the production method of the present invention, by using a combination of dry heat treatment and gibberellin treatment, the seed germination rate of bovine serrata is further improved and root elongation is good, and the long-term stable growth rate of serrata seedlings is remarkably improved. of high quality seedlings can be mass-produced.

According to another object of the present invention, the present invention provides an excellent seedling produced by the above production method.

The excellent seedlings of the cypress tree according to the present invention are excellent seedlings that can grow stably for a long period of time and are suitable for large-scale afforestation.

In the excellent seedlings of the present invention, the germination rate at 210 days after seed sowing is 78% or more, and the growth rate at 306 days after sowing is 66% or more.

According to the method for mass production of high-quality seedlings according to the present invention, it improves the root elongation while increasing the germination rate of the seedlings, and also significantly improves the long-term stable growth rate of the germinated seedlings. It is possible to produce large quantities of high-quality seedlings of pine trees that are resistant to stress such as overcrowding and excessive fertilization.

In addition, since the excellent seedlings of the cypress tree according to the present invention are healthy seedlings with good long-term growth, they can be utilized for mass afforestation.

1 is a photograph of parameters obtained by collecting Sogwi tree seeds.
Fig. 2 is a photograph of the collected Sogwi tree fruit (including seeds).
3 is a photograph of the process of dry heat treatment of Sogwi tree seeds.
Figure 4 is a photograph of the process of treating the seeds of bovine gibberellin and distilled water.
5 is a photograph of Sogwi tree seeds used for sowing.
6 is a photograph of the seedlings of Sogwi tree germinated after sowing.
7A is a graph showing the germination rate (210 days) of Sogwi tree seeds according to the seed treatment.
Figure 7b is a graph showing the change in the survival rate of the seedlings of the cypress seedling according to the seed treatment.
8 is a photograph showing the state of the root before transplantation of the seedlings of Sogwi tree.
Fig. 9 is a photograph showing the growth state of a transplanted cypress tree.

Hereinafter, the configuration and effect of the present invention will be described in more detail with reference to specific examples in order to help the understanding of the present invention. However, the following examples and experimental examples are merely illustrative in order to understand the present invention more clearly, and the scope of the present invention is not limited by the following examples.

Preparation Example 1: Sterilization of the seeds of Sogwi tree

On June 17, 2019, the fruit was collected from the Sogwipo tree community in Seogwipo, Jeju Island, the flesh was removed, and the seeds were obtained. The seeds were immersed in running water for 5 days and stored at 4℃ for 100 days, and 10 in 1% NaOCl. After processing for minutes, washing with running water 3 times, and then washing with distilled water once to complete sterilization (FIGS. 1 and 2).

Experimental Example 1: Analysis of enhancement of the germination rate of Sogwi tree

As shown in Table 1 below, the sterilized bovine oak seeds sterilized in Preparation Example 1 were divided into 3 groups (Comparative Example 1, Comparative Example 2 and Example 1), and the seeds of Comparative Example 1 were placed in 1,000 ppm gibberellin aqueous solution for 20 hours and 30 minutes. Only the immersion treatment was performed, and the seeds of Comparative Example 2 were immersed in distilled water for 54 hours and 50 minutes in a dry oven at 40 ° C for 54 hours and 50 minutes and then immersed in distilled water for 20 hours and 30 minutes, and the seeds of Example 1 were dried in a dry oven at 40 ° C for 54 hours and 50 minutes After treatment, immersion treatment was performed for 20 hours and 30 minutes in 1,000 ppm gibberellin aqueous solution ( FIGS. 3 , 4 and 5 ).

number of seeds dry heat treatment Gibberellin treatment Comparative Example 1 100 × ○ Comparative Example 2 100 ○ × Example 1 100 ○ ○

The seeds treated as described above were sown and germinated on horticultural soil in a shaded greenhouse on October 11, 2019, and the germination rate of each group was calculated on the 210th day after sowing and shown in FIG. 7A.

As shown in FIG. 7a , the germination rate of Sogwi tree seeds of Comparative Example 1 (gibberellin treatment group) was 74%, and that of Comparative Example 2 (dry heat treatment group) was 56%. According to these results, it can be confirmed that the gibberellin treatment effectively enhances the germination rate of bovine serrata seeds, and that the dry heat treatment lacks the effect of enhancing the germination rate of pine seeds.

On the other hand, the germination rate of the seeds of bovine oak in Example 1 (dry heat treatment + gibberellin treatment) was 78%, confirming that it was further improved compared to Comparative Example 1 (gibberellin treatment group).

In addition, the seedlings of Example 1 also had good root elongation (FIG. 8).

Experimental Example 2: Production of excellent seedlings

In Experimental Example 1, the germinated bovine seedlings of Comparative Example 1, Comparative Example 2, and Example 1 were transplanted into the shaded soil on the 237th day (June 3, 2020) after sowing and continued to grow, after sowing 237 The growth rates of each group were calculated on days, 270 days and 306 days, and are shown in Table 2 and Figure 7b.

Growth rate (%) 210 days 237 days 270 days 306 days Comparative Example 1 74 32 30 29 Comparative Example 2 56 52 47 46 Example 1 78 66 66 66

As shown in Table 2 and Figure 7b, in the case of Example 1, which was treated with gibberellin after dry heat treatment according to the present invention, the survival rate of the seedlings of cypress tree was 66% at 237 days, 66% at 270 days, and 66% at 306 days. It can be seen that, after transplantation, all the seedlings of the cypress tree are not withered at all, and it can be confirmed that the high growth rate is maintained stably for a long period of time.

On the other hand, in the case of the small gibberellin seedlings of Comparative Example 1 treated only with gibberellin, the survival rate was 32% at 237 days, 30% at 270 days, and 29% at 306 days. You can see this decline.

In addition, in the case of the seedlings of Comparative Example 2 treated only with dry heat, the survival rate was 52% at 237 days, 47% at 270 days, and 46% at 306 days, confirming that the survival rate continued to decrease.

Comparing the long-term survival rate of seedlings of cypress seedlings 306 days after sowing, it can be seen that the case of Example 1 was 66%, which was improved by about 2.3 times and 1.5 times compared to Comparative Example 1 and Fertilizer system 2 (29% and 46%, respectively). can

From the above results, when using a combination of dry heat treatment and gibberellin treatment according to the present invention, it is possible to improve the root elongation while enhancing the germination rate of bovine tree seeds, and to significantly improve the long-term stable growth rate of bovine oak seedlings. It can be seen that high-quality seedlings can be mass-produced.

Claims (11)

i) sterilizing the seeds of the bovine oak;
ii) dry heat treatment of the sterilized seeds at 35-45° C. for 15-55 hours;
iii) immersing the dry heat-treated seeds in an aqueous solution of gibberellin at a concentration of 500 to 1,500 ppm for 20 to 24 hours;
iv) sowing and germinating the sowing seeds from step iii); and
v) growing the germinated seedlings to obtain excellent seedlings of serrata.
The method of claim 1, wherein the sterilization in step i) is to immerse the seeds of the bovine serrata in running water for 5 days, then treat them in 1% sodium hypochlorite for 10 minutes, wash them 3 times in running water, and then wash them once with distilled water. A method of mass production of excellent seedlings of spruce which is carried out.
The method according to claim 1, wherein the dry heat treatment in step ii) is performed using a dry heat machine or a dry oven.
The method of claim 1, wherein the dry heat treatment in step ii) is performed at 40° C. for 54 hours and 50 minutes.
The method of claim 1, wherein the concentration of gibberellin in step iii) is 1,000 ppm.
The method of claim 1, wherein the sowing in step iv) is carried out under light-shielding conditions at a temperature of 12 to 27°C.
The method of claim 1, wherein the growth in step v) is carried out by growing for 10 to 24 months after sowing at a temperature of 0.5 to 43.5° C. under light-shielding conditions.
The method according to claim 1, wherein in step v), the seedlings are transplanted into new soil and grown 6 to 8 months after sowing.
[Claim 9] A cypress seedling produced by the method according to any one of claims 1 to 8.
10. The excellent seedling of claim 9, wherein the germination rate is 78% or more on the 210th day after sowing.
10. The excellent seedling of claim 9, wherein the growth rate is 66% or more on the 306th day after sowing.

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