KR20210060801A - Cosmetic Compositions for Anti-aging Comprising Complex Fermented Product of Plants - Google Patents
Cosmetic Compositions for Anti-aging Comprising Complex Fermented Product of Plants Download PDFInfo
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- KR20210060801A KR20210060801A KR1020190148249A KR20190148249A KR20210060801A KR 20210060801 A KR20210060801 A KR 20210060801A KR 1020190148249 A KR1020190148249 A KR 1020190148249A KR 20190148249 A KR20190148249 A KR 20190148249A KR 20210060801 A KR20210060801 A KR 20210060801A
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- plant complex
- fermented
- mulberry
- cosmetic composition
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Classifications
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
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- A—HUMAN NECESSITIES
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/522—Antioxidants; Radical scavengers
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Dermatology (AREA)
- Microbiology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Mycology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Cosmetics (AREA)
Abstract
Description
본 발명은 식물 복합 발효물을 유효성분으로 포함하는 항노화 화장료 조성물에 관한 것이다.The present invention relates to an anti-aging cosmetic composition comprising a plant complex fermented product as an active ingredient.
생체 외부로부터 유입되거나, 생체 내에서 발생하는 활성산소는 생체의 노화를 촉진시키거나, 암 발생의 원인이 되기도 한다. 따라서 활성산소에 의한 산화를 억제하는 항산화 물질에 대한 연구가 진행되며, 항산화 물질은 페놀성 화합물, 플라보노이드, 토코페롤, 비타민 C, 셀레늄 등이 알려져 있다. 그러나 천연에 존재하는 항산화 물질은 피부에 적용할 경우 실질적으로 충분한 효과를 기대할 수 없는 실정이다. 반면, 항산화력이 뛰어나고 저가의 합성 항산화제는 인체 부작용 등 안전성에 대한 우려로 그 사용이 제한되는 문제점이 있다. Free radicals introduced from outside the body or generated in the body promote aging in the body or cause cancer. Therefore, research on antioxidants that inhibit oxidation by free radicals is being conducted, and antioxidants are known as phenolic compounds, flavonoids, tocopherols, vitamin C, selenium, and the like. However, when applied to the skin, antioxidant substances that exist in nature cannot be expected to have a substantial effect. On the other hand, synthetic antioxidants having excellent antioxidant power and low cost have a problem that their use is limited due to concerns about safety such as side effects on the human body.
주름이 형성되는 원인은 자연적 노화에 의한 주름의 생성과 자외선 노출(광노화)에 의한 주름의 생성으로 크게 나눌 수 있는데, 두 가지 원인에 의한 주름 형성 메커니즘은 아직까지 정확하게 밝혀져 있지 않은 상태이다. 자연적 노화와 광노화에 의한 피부변화와 관련되어 발견되는 공통적인 현상은 진피 내의 주 구성단백질인 콜라겐 및 엘라스틴의 감소 및 변형, 진피의 기질인 히알루론산의 감소에 의한 피부 탄력 감소이다. 특히 지금까지의 연구결과 의하면 진피 내 주 단백질인 콜라겐 및 엘라스틴의 감소 및 변형이 주름의 생성 및 피부의 탄력저하에 직접적으로 관여하는 것으로 알려져 있다. 콜라겐은 피부의 섬유아세포에서 생성되는 주요 기질 단백질로서 세포외 간질에 존재하고, 중요한 기능으로는 피부의 기계적 견고성, 결합조직의 저항력과 조직의 결합력, 세포 접착의 지탱, 세포분할과 분화(유기체의 성장 혹인 상처 치유시)의 유도 등이 알려져 있다. 이러한 콜라겐은 연령 및 자외선 조사에 의한 광노화에 의해 감소하며, 이는 피부의 주름 형성과 밀접한 연관이 있다고 알려져 있다. 또한 근래에 들어 피부 노화에 대한 광범위한 연구 결과가 보고되면서 피부에서의 콜라겐의 중요한 기능이 밝혀지고 있다. 콜라겐 합성을 촉진하여 주름개선 효과를 나타내는 유효성분들이 알려져 있다. 예를 들어, 레티노산(retinoic acid), TGF(Transforming growth factor), 동물 태반 유래의 단백질, 베툴린산(betulinic acid), 클로렐라 추출물 등이 콜라겐 합성 촉진 물질로서 알려져 있다.The causes of wrinkle formation can be largely divided into wrinkle formation due to natural aging and wrinkle formation due to UV exposure (photoaging), and the mechanism of wrinkle formation due to the two causes has not yet been accurately identified. Common phenomena found in relation to skin changes caused by natural aging and photoaging are the reduction and transformation of collagen and elastin, the main constituent proteins in the dermis, and reduction of skin elasticity due to the reduction of hyaluronic acid, the matrix of the dermis. In particular, according to the results of the studies so far, it is known that the reduction and transformation of collagen and elastin, the main proteins in the dermis, are directly involved in the formation of wrinkles and the reduction of elasticity of the skin. Collagen is a major matrix protein produced by fibroblasts of the skin and is present in the extracellular interstitial. Its important functions include skin mechanical firmness, connective tissue resistance and tissue bonding, support for cell adhesion, cell division and differentiation (organic Growth or induction of wound healing) is known. This collagen is reduced by age and photoaging caused by UV irradiation, which is known to be closely related to the formation of wrinkles in the skin. In addition, as the results of extensive research on skin aging have recently been reported, an important function of collagen in the skin has been revealed. Active ingredients that promote collagen synthesis to improve wrinkles are known. For example, retinoic acid, TGF (transforming growth factor), animal placenta-derived proteins, betulinic acid, and chlorella extract are known as collagen synthesis promoting substances.
일반적으로 피부 미백과 가장 밀접하게 연관되어 있는 것은 멜라닌으로 알려져 있다. 사람의 피부색은 피부 내부의 멜라닌(Melanin)의 함량, 분포에 따라 결정되는데, 유전적인 요인 외에도 태양 자외선이나 피로, 스트레스 등의 환경적 또는 생리적 조건에 의해서도 영향을 받는다. 멜라닌은 피부 표피층에 있는 멜라노사이트(Melanocyte)에서 합성되는데, 멜라노사이트 내 소기관인 멜라노좀(Melanosome)에서 아미노산의 일종인 티로신(Tyrosine)에 티로시나제(Tyrosinase)라는 효소가 작용하여 도파(DOPA), 도파퀴논(Dopaquinone)으로 바뀐 후 비효소적인 산화반응을 거쳐 만들어진다. 이와 같은 멜라닌의 합성이 피부 내에서 과도하게 일어나면, 피부 톤을 어둡게 하고, 기미, 주근깨, 노인성 흑자, 모반 등을 발생시킨다. 따라서, 피부 내의 티로시나제 활성을 저해하여 멜라닌 색소의 합성을 저해시키면, 피부 톤을 밝게 하여 피부 미백을 실현할 수 있을 뿐만 아니라 자외선, 호르몬 및 유전적인 원인에 기인하여 발생하는 기미, 주근깨 등의 피부 과색소 침착증을 개선시킬 수 있다.In general, it is known as melanin that is most closely associated with skin whitening. Human skin color is determined by the content and distribution of melanin inside the skin. In addition to genetic factors, it is affected by environmental or physiological conditions such as solar ultraviolet rays, fatigue, and stress. Melanin is synthesized from melanocytes in the epidermis of the skin, and an enzyme called tyrosinase acts on tyrosine, a kind of amino acid in the melanosome, an organelle within the melanocyte It is made through a non-enzymatic oxidation reaction after being converted to quinone. When such melanin synthesis occurs excessively in the skin, skin tone is darkened, and spots, freckles, senile blacks, and birthmarks are generated. Therefore, by inhibiting the synthesis of melanin pigment by inhibiting the tyrosinase activity in the skin, not only can the skin tone be brightened to realize skin whitening, but also skin hyperpigmentation such as spots and freckles caused by ultraviolet rays, hormones and genetic causes. It can improve calmness.
최근 기술의 발달과 삶의 질 향상으로 미(美)에 대한 욕구와 관심이 증대되면서 과도한 멜라닌 생성을 막는 미백 제품 개발이 필요하게 되었고, 이에 따라 미백제 및 미백 화장품 개발에 관한 연구가 활발히 진행 중이다. 멜라닌 침착 질환들을 치료하기 위해 오늘날 여러 미백 물질들이 사용되고 있으나 이들 대부분은 멜라닌형성세포에 독성을 나타내며 여러 부작용들을 유발한다. 예를 들면, 미백 효과가 우수한 하이드로퀴논(Hydroquinone)은 세포독성이 있으며, 신장독성 및 발암 가능성이 있는 것으로 알려져 있다. 또한, 조직흑변증(Ochonosis)과 같은 피부손상으로 인해 유럽에서는 미백화장품에 사용이 금지되어 있다. 코직산(Kojic acid)은 미백효과가 우수하지만 세포독성이 있으며, 접촉성 피부염 또는 암을 일으킬 수 있는 것으로 알려져 있다. As the desire and interest in beauty has increased due to the recent development of technology and the improvement of the quality of life, it has become necessary to develop a whitening product that prevents excessive melanin production, and accordingly, research on the development of whitening agents and whitening cosmetics is actively underway. Several whitening substances are used today to treat melanogenesis disorders, but most of them are toxic to melanogenic cells and cause several side effects. For example, hydroquinone, which has excellent whitening effect, has cytotoxicity, and is known to have renal toxicity and carcinogenic potential. In addition, due to skin damage such as tissue blackness (Ochonosis), it is prohibited to use in whitening cosmetics in Europe. Kojic acid is known to have excellent whitening effect, but it is cytotoxic and can cause contact dermatitis or cancer.
피부는 환경의 변화나 내부의 요인에 의한 스트레스 및 외부 요인에 의한 자극 등으로 민감해지며 수분 보유력이 저하될 수 있으며, 노화가 진행됨에 따라 각질층의 수분이 감소하여 피부가 건조해지고 윤기를 잃어 칙칙해 보이는 등의 현상이 발생한다. 피부의 수분이 감소하는 경우 피부는 건조하게 되며 탄력이 떨어지고 주름 생성이 이루어지게 된다. 이와 더불어 피부 톤도 어두워지며 피부 보호막 기능이 저하되어 외부의 자극에도 더욱 민감한 상태가 되어 각종 트러블이 증가할 수 있다. 이처럼 각질층의 보습 상태는 피부의 외형(피부의 유연성 및 거친 정도)과 직접적으로 연관되어 있으며, 각질층의 피부 수분 함량은 피부의 컨디션 및 투과성과 관련되는 피부 막 항상성에 영향을 미치므로, 화장품 또는 바르는 의약품의 흡습도가 달라질 수 있어 피부 보습이 중요한 요소로 작용하고 있다. 따라서 피부가 보습 및 탄력을 잃지 않도록 피부의 보습력을 유지해주고, 그 효과를 지속시키는 화장료 조성물에 대한 요구가 증가하는 추세이다.The skin becomes sensitive to environmental changes, stress caused by internal factors, and irritation caused by external factors, and moisture retention may decrease. As aging progresses, the moisture in the stratum corneum decreases, resulting in dryness and loss of shine, resulting in dull skin. Symptoms such as visible occur. When the moisture in the skin decreases, the skin becomes dry, the elasticity decreases, and wrinkles are formed. In addition, the skin tone becomes darker and the skin protective film function is deteriorated, making it more sensitive to external stimuli, which can increase various troubles. As such, the moisturizing state of the stratum corneum is directly related to the appearance of the skin (the softness and roughness of the skin), and the skin moisture content of the stratum corneum affects the skin membrane homeostasis related to the condition and permeability of the skin. The moisture absorption of medicines can vary, so skin moisturization is acting as an important factor. Accordingly, there is a trend of increasing demand for cosmetic compositions that maintain the moisturizing power of the skin so that the skin does not lose moisture and elasticity, and that sustain the effect.
이와 같이, 다양한 분야에서 항산화, 주름개선, 미백 및 보습 효과를 가지는 연구 및 개발이 활발하게 진행되고 있으며, 특히 피부에 독성이나 자극을 주지 않기 위하여 천연 물질을 이용한 연구가 계속되고 있다.As described above, research and development having antioxidant, wrinkle improvement, whitening and moisturizing effects are being actively conducted in various fields, and in particular, studies using natural substances are being continued in order not to cause toxicity or irritation to the skin.
식물 추출물을 이용하는 화장료 조성물 기술로는, 국내의 경우 대한민국 등록특허공보 제10-1789446호「향부자 유래 발렌센을 유효성분으로 포함하는 아토피 피부염 치료용 약학적 조성물」, 대한민국 등록특허공보 제10-2001236호「도인 추출물을 유효성분으로 포함하는 미생물 균총 개선용 조성물」, 대한민국 등록특허공보 제10-1781111호「후박, 시호 및/또는 형개 추출물을 함유하는 화장료 조성물」, 대한민국 등록특허공보 제10-0882744호「목단피 추출물을 함유하는 화장료 조성물의 제조방법」등이 개시되어 있다.As a cosmetic composition technology using plant extracts, in Korea, Korean Patent Publication No. 10-1789446 ``Pharmaceutical composition for treating atopic dermatitis containing valencene derived from fragrant extract as an active ingredient'', Korean Patent Publication No. 10-2001236 Ho "Composition for improving microbial flora containing Doin extract as an active ingredient", Korean Patent Publication No. 10-1781111, "Cosmetic composition containing extracts of Hubak, Siho and/or Hyeonggae", Korean Registered Patent Publication No. 10-0882744 No. "Method for producing a cosmetic composition containing Mokdanpi extract" and the like are disclosed.
이에, 본 발명자들은 도인(Prunus persica (Linn) Batsch), 목단피(Paeomia suffruticosa), 향부자(Cyperus rotundus) 및 후박(Magnolia officinalis)의 식물 복합 발효물이 우수한 DPPH 소거 활성, 콜라겐 생성, 콜라게나아제 억제 효능, 멜라닌 생성 저해 효능 및 히알루론산 생성 효능을 나타내어, 항산화, 주름개선, 미백 및 보습 효과를 동시에 부여할 수 있다는 사실을 발견하고 본 발명을 완성하게 되었다.Thus, the present inventors have degrees (Prunus persica (Linn) Batsch) , mokdanpi (Paeomia suffruticosa), Cyperus rotundus (Cyperus rotundus), and Magnolia plant complex fermentation excellent DPPH scavenging activity, collagen production (Magnolia officinalis), collagenase inhibition By showing the efficacy, melanin production inhibitory effect and hyaluronic acid production effect, it was discovered that it can simultaneously provide antioxidant, wrinkle improvement, whitening and moisturizing effects, and the present invention was completed.
본 발명의 목적은 천연물 유래의 항노화 화장료 조성물을 제공하는 데 있다.An object of the present invention is to provide an anti-aging cosmetic composition derived from natural products.
본 발명의 또 다른 목적은 천연물 유래의 항노화 화장료 조성물의 제조방법을 제공하는 데 있다.Another object of the present invention is to provide a method for preparing an anti-aging cosmetic composition derived from natural products.
상기와 같은 목적을 달성하기 위하여, 본 발명은 도인(Prunus persica (Linn) Batsch), 목단피(Paeomia suffruticosa), 향부자(Cyperus rotundus) 및 후박(Magnolia officinalis)의 식물 복합 추출물을 오디 발효물로 발효시켜 수득된 식물 복합 발효물을 유효성분으로 포함하는 항노화 화장료 조성물을 제공한다.In order to achieve the above object, the present invention is a plant complex extract of Doin (Prunus persica (Linn) Batsch ), Mokdanpi (Paeomia suffruticosa ), Hyangbuja (Cyperus rotundus ) and Hubak ( Magnolia officinalis ) by fermenting it with a fermented mulberry. It provides an anti-aging cosmetic composition comprising the obtained plant complex fermented product as an active ingredient.
본 발명에 있어서, 상기 식물 복합 추출물은 조성물 총 중량에 대하여 도인(Prunus persica (Linn) Batsch) 10 내지 50중량%, 목단피(Paeomia suffruticosa) 10 내지 50중량%, 향부자(Cyperus rotundus) 10 내지 50중량% 및 후박(Magnolia officinalis) 10 내지 50중량% 포함되는 것을 특징으로 한다.In the present invention, the plant complex extract is Doin ( Prunus persica (Linn) Batsch ) 10 to 50% by weight, Mokdanpi (Paeomia suffruticosa ) 10 to 50% by weight, Cyperus rotundus 10 to 50% by weight based on the total weight of the composition. % And Hubak ( Magnolia officinalis ) characterized in that it contains 10 to 50% by weight.
본 발명에 있어서, 상기 추출물은 물, 탄소수 1 내지 4의 저급 알코올, 프로필렌글리콜, 글리세린 및 이들의 혼합용매로 이루어진 군으로부터 선택되는 용매로 추출되는 것을 특징으로 한다.In the present invention, the extract is characterized in that it is extracted with a solvent selected from the group consisting of water, a lower alcohol having 1 to 4 carbon atoms, propylene glycol, glycerin, and a mixed solvent thereof.
본 발명에 있어서, 상기 오디 발효물은 오디와 설탕을 항아리에 넣고 발효시켜 수득된 발효오디청 및 발효오디박인 것을 특징으로 한다.In the present invention, the fermented mulberry product is characterized in that it is a fermented mulberry and fermented mulberry paste obtained by fermenting the mulberry and sugar in a jar.
본 발명에 있어서, 상기 식물 복합 발효물은 조성물 총 중량에 대하여 0.001 내지 20 중량% 포함되는 것을 특징으로 한다.In the present invention, the plant complex fermentation product is characterized in that it contains 0.001 to 20% by weight based on the total weight of the composition.
본 발명에 있어서, 상기 식물 복합 발효물은 DPPH 소거 활성, 콜라겐 생성, 콜라게나아제 억제 효능, 멜라닌 생성 저해 효능 및 히알루론산 생성 효능을 갖는 것을 특징으로 한다.In the present invention, the plant complex fermentation product is characterized by having DPPH scavenging activity, collagen production, collagenase inhibitory effect, melanin production inhibitory effect, and hyaluronic acid production effect.
또한, 본 발명은 오디 및 설탕을 항아리에 넣고 혼합하여 1차 발효시키는 단계; 상기 1차 발효된 오디 발효물을 여과하여 발효오디청 및 발효오디박을 각각 분리하는 단계; 상기 발효오디청과 도인(Prunus persica (Linn) Batsch), 목단피(Paeomia suffruticosa), 향부자(Cyperus rotundus) 및 후박(Magnolia officinalis)의 식물 복합 추출물을 항아리에 넣고 혼합한 다음 정제수를 첨가하는 단계; 및 상기 발효오디박 및 정제수를 항아리에 넣어 혼합한 다음, 천으로 항아리를 씌운 후 2차 발효시키는 단계를 포함하는 것을 특징으로 하는 식물 복합 발효물을 유효성분으로 포함하는 항노화 화장료 조성물의 제조방법을 제공한다.In addition, the present invention comprises the steps of first fermenting by mixing mulberry and sugar in a jar; Filtering the first fermented mulberry fermented product to separate the fermented mulberry and the fermented mulberry, respectively; Putting and mixing the plant complex extracts of the fermented Audicheong and Doin ( Prunus persica (Linn) Batsch ), Mokdanpi (Paeomia suffruticosa), Hyangbuja (Cyperus rotundus ), and Magnolia officinalis in a jar, and then adding purified water; And mixing the fermented mulberry paste and purified water in a jar, covering the jar with a cloth, and performing secondary fermentation. Provides.
본 발명에 있어서, 상기 1차 발효는 오디 및 설탕을 1:1~2의 비율로 항아리에 넣고 12 내지 36 시간 후, 하루에 한번씩 저어주면서 15 내지 25℃에서 7 내지 42일 동안 발효 및 숙성시키는 것을 특징으로 한다.In the present invention, in the first fermentation, mulberry and sugar are put into a jar in a ratio of 1:1 to 2, and after 12 to 36 hours, fermentation and aging at 15 to 25° C. for 7 to 42 days while stirring once a day It is characterized by that.
본 발명에 있어서, 상기 식물 복합 추출물은 조성물 총 중량에 대하여 도인(Prunus persica (Linn) Batsch) 10 내지 50중량%, 목단피(Paeomia suffruticosa) 10 내지 50중량%, 향부자(Cyperus rotundus) 10 내지 50중량% 및 후박(Magnolia officinalis) 10 내지 50중량% 포함된 식물 복합물을 용매에 혼합한 다음, 50 내지 60℃에서 1 내지 6시간 동안 추출하여 수득된 것을 특징으로 한다.In the present invention, the plant complex extract is Doin ( Prunus persica (Linn) Batsch ) 10 to 50% by weight, Mokdanpi (Paeomia suffruticosa ) 10 to 50% by weight, Cyperus rotundus 10 to 50% by weight based on the total weight of the composition. % And Hubak ( Magnolia officinalis ) is characterized in that obtained by mixing the plant complex containing 10 to 50% by weight in a solvent, and then extracted for 1 to 6 hours at 50 to 60 ℃.
본 발명에 있어서, 상기 2차 발효는 식물 복합 추출물 100 중량부에 대하여 발효오디청 1000 내지 3000 중량부를 항아리에 넣고 혼합한 다음 정제수 4000 내지 2000 중량부를 첨가한 후, 발효오디박 1000 내지 3000 중량부 및 정제수 4000 내지 2000 중량부를 항아리에 넣어 혼합한 다음, 천으로 항아리를 씌운 후 10 내지 25℃에서 7일 내지 42일 동안 발효 및 숙성시키는 것을 특징으로 한다.In the present invention, in the second fermentation, 1000 to 3000 parts by weight of fermented mulberry extract are added to a jar and mixed, and 4000 to 2000 parts by weight of purified water are added, and then 1000 to 3000 parts by weight of fermented mulberry extract, And 4000 to 2000 parts by weight of purified water is put into a jar and mixed, and then the jar is covered with a cloth, and then fermented and aged at 10 to 25°C for 7 to 42 days.
본 발명으로부터 제공되는 식물 복합 발효물은 DPPH 소거 활성, 콜라겐 생성, 콜라게나아제 억제 효능, 멜라닌 생성 저해 효능 및 히알루론산 생성 효능이 우수하기 때문에, 항산화, 주름개선, 미백 및 보습용 화장료 조성물로 활용 가능하다.The plant complex fermented product provided by the present invention has excellent DPPH scavenging activity, collagen production, collagenase inhibitory effect, melanin production inhibitory effect, and hyaluronic acid production effect, so it is used as a cosmetic composition for antioxidant, wrinkle improvement, whitening and moisturizing. It is possible.
다른 식으로 정의되지 않는 한, 본 명세서에서 사용된 모든 기술적 및 과학적 용어들은 본 발명이 속하는 기술분야에서 숙련된 전문가에 의해서 통상적으로 이해되는 것과 동일한 의미를 가진다. 일반적으로, 본 명세서에서 사용된 명명법 은 본 기술분야에서 잘 알려져 있고 통상적으로 사용되는 것이다.Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by an expert skilled in the art to which the present invention belongs. In general, the nomenclature used in this specification is well known and commonly used in the art.
본원 명세서 전체에서, 어떤 부분이 어떤 구성 요소를 "포함" 한다고 할 때, 이는 특별히 반대되는 기재가 없는 한 다른 구성 요소를 제외하는 것이 아니라 다른 구성요소를 더 포함할 수 있는 것을 의미한다. In the entire specification of the present application, when a part "includes" a certain component, it means that other components may be further included rather than excluding other components unless otherwise specified.
본 발명의 일 구현예에 따르면, 본 발명은 도인(Prunus persica (Linn) Batsch), 목단피(Paeomia suffruticosa), 향부자(Cyperus rotundus) 및 후박(Magnolia officinalis)의 식물 복합 추출물을 오디 발효물로 발효시켜 수득된 식물 복합 발효물을 유효성분으로 포함하는 항노화 화장료 조성물에 관한 것이다.According to an embodiment of the present invention, the present invention is a plant complex extract of Doin (Prunus persica (Linn) Batsch ), Mokdanpi (Paeomia suffruticosa ), Hyangbuja (Cyperus rotundus ) and Hubak ( Magnolia officinalis ) by fermenting it with a fermented mulberry. It relates to an anti-aging cosmetic composition comprising the obtained plant complex fermented product as an active ingredient.
상기 식물 복합 추출물은 조성물 총 중량에 대하여 도인(Prunus persica (Linn) Batsch) 10 내지 50중량%, 목단피(Paeomia suffruticosa) 10 내지 50중량%, 향부자(Cyperus rotundus) 10 내지 50중량% 및 후박(Magnolia officinalis) 10 내지 50중량% 포함되 것이 바람직하다. 만일, 어느 한 식물의 함량이 10중량% 미만인 경우에는 그 식물의 함량이 상대적으로 낮아 본래 목적하는 생리활성 효과를 상대적으로 충분하게 달성하지 못할 우려가 있으며, 어느 한 식물의 함량이 50중량%를 초과하는 경우에는 상대적으로 다른 식물의 함량이 낮아져 본래 목적하는 생리활성 효과를 상대적으로 충분하게 달성하지 못할 우려가 있기 때문에 바람직하지 않다.The plant complex extract is doin ( Prunus persica (Linn) Batsch ) 10 to 50% by weight, Mokdanpi (Paeomia suffruticosa ) 10 to 50% by weight, Cyperus rotundus 10 to 50% by weight, and Magnolia officinalis ) It is preferable to contain 10 to 50% by weight. If the content of any one plant is less than 10% by weight, the content of the plant is relatively low, and there is a fear that the original desired physiological activity effect may not be sufficiently achieved, and the content of any one plant is 50% by weight. If the amount is exceeded, the content of other plants is relatively lowered, which is not preferable because there is a fear that the desired physiological activity effect may not be achieved relatively sufficiently.
본 발명에 따른 추출물은 본 발명이 속한 분야에 공지된 다양한 추출방법을 통해 얻어질 수 있고, 구체적으로는 물, 탄소수 1 내지 4의 저급 알코올, 프로필렌글리콜, 글리세린 및 이들의 혼합용매로 이루어진 군으로부터 선택되는 용매로 추출되는 것일 수 있다. The extract according to the present invention can be obtained through various extraction methods known in the field to which the present invention belongs, and specifically, from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, propylene glycol, glycerin, and a mixed solvent thereof It may be extracted with a selected solvent.
구체적으로, 추출용매의 함량은 식물 복합물 고형분 중량의 2 내지 100배, 더욱 바람직하게는 5 내지 50배, 가장 바람직하게는 10 내지 20배인 것일 수 있다.Specifically, the content of the extraction solvent may be 2 to 100 times, more preferably 5 to 50 times, most preferably 10 to 20 times the weight of the solid content of the plant complex.
또한, 상기 추출물은 추출물의 유효 성분이 파괴되지 않거나 파괴가 최소화된 조건에서 실온 또는 가온하여 추출할 수 있다. 추출 방법은 특별히 제한되지 않고 추출물의 종류와 추출하는 방법에 따라 초음파 추출, 냉침 추출, 초임계 추출, 초고압 추출, 환류 냉각 추출, 상온 추출, 열수 추출 등을 이용할 수 있다.In addition, the extract may be extracted by heating or at room temperature under conditions in which the active ingredient of the extract is not destroyed or the destruction is minimized. The extraction method is not particularly limited, and ultrasonic extraction, cold needle extraction, supercritical extraction, ultra-high pressure extraction, reflux cooling extraction, room temperature extraction, hot water extraction, and the like may be used depending on the type and extraction method of the extract.
여과는 추출액으로부터 부유하는 고체 입자를 제거하는 과정으로 면, 나일론, 종이 등을 이용하여 입자를 걸러 내거나 한외여과법, 냉동여과법, 원심분리법 등을 이용할 수 있으나 이에 제한되지 않는다.Filtration is a process of removing suspended solid particles from the extract, and filtering particles using cotton, nylon, paper, etc., or ultrafiltration, cryofiltration, centrifugation, etc. may be used, but is not limited thereto.
여액을 농축한 다음 건조하는 단계는 동결건조, 진공건조, 열풍건조, 분무건조, 감압건조, 포말건조, 고주파건조, 적외선건조 등을 포함하나 이에 제한되지 않는다. 경우에 따라 최종 건조된 추출물을 분쇄하는 공정을 추가할 수 있다.The step of concentrating and then drying the filtrate includes, but is not limited to, freeze drying, vacuum drying, hot air drying, spray drying, vacuum drying, foam drying, high frequency drying, infrared drying, and the like. In some cases, a process of pulverizing the final dried extract may be added.
본 발명의 일 구현예에 따르면, 상기 추출물은 발효액, 상기 추출액을 건조시킨 분말, 상기 추출액을 농축여과한 농축물 등 다양한 형태로 가공되어 이용될 수 있다. According to one embodiment of the present invention, the extract may be processed and used in various forms, such as a fermentation broth, a powder obtained by drying the extract, and a concentrate obtained by concentrating and filtering the extract.
본 발명의 일 구현예에 따르면, 상기 식물 복합 발효물은 도인(Prunus persica (Linn) Batsch), 목단피(Paeomia suffruticosa), 향부자(Cyperus rotundus) 및 후박(Magnolia officinalis)의 식물 복합 추출물을 오디 발효물로 발효시켜 수득된 것이며, 이때, 오디 발효물은 오디와 설탕을 항아리에 넣고 발효시켜 수득된 발효오디청 및 발효오디박인 것일 수 있다. According to an embodiment of the present invention, the plant complex fermentation is a plant complex extract of Doin (Prunus persica (Linn) Batsch ), Mokdanpi (Paeomia suffruticosa ), Hyangbuja (Cyperus rotundus ), and Magnolia officinalis. It is obtained by fermentation with, in this case, the fermented mulberry may be a fermented mulberry and a fermented mulberry obtained by fermenting the mulberry and sugar in a jar.
이때, 발효오디청은 여과하여 걸러진 액을 의미하고, 발효오디박은 여과하고 남은 부산물을 의미한다. 상기 발효오디청과 발효오디박은 천연 미생물과 발효 효소가 포함된 오디 발효물로서 식물 복합 추출물을 발효시키는 목적으로 사용된다.At this time, fermented mulberry cheong refers to the filtered liquid, and fermented mulberry meal refers to the remaining by-products after filtering. The fermented mulberry and fermented mulberry are fermented mulberry products containing natural microorganisms and fermenting enzymes, and are used for the purpose of fermenting complex plant extracts.
본 발명의 일 구현예에 따르면, 상기와 같은 방법으로부터 수득된 식물 복합 발효물은 조성물 총 중량에 대하여 0.001 내지 20 중량% 포함될 수 있고, 더욱 바람직하게는 0.1 내지 1 중량% 포함될 수 있다. 상기 발효물이 조성물의 총 중량에 대하여 0.001 중량% 미만일 경우에는 주름개선, 미백 및 보습 효과를 충분히 얻을 수 없기 때문에 조성물로서의 기능이 떨어지고, 20 중량%를 초과하는 경우에는 그 이상을 함유하지 않아도 충분한 효능을 나타낼 수 있기 때문에 비경제적일 뿐만 아니라, 제형의 안정성 및 발림성 등이 저하되어 화장품으로 사용에 어려움이 있기 때문에 바람직하지 않다.According to an embodiment of the present invention, the plant complex fermented product obtained from the above method may be included in an amount of 0.001 to 20% by weight, more preferably 0.1 to 1% by weight, based on the total weight of the composition. If the fermented product is less than 0.001% by weight based on the total weight of the composition, the function as a composition is deteriorated because the wrinkle improvement, whitening and moisturizing effect cannot be sufficiently obtained, and if it exceeds 20% by weight, it is sufficient even if it does not contain more. Not only is it uneconomical because it can exhibit efficacy, and it is not preferable because it is difficult to use as a cosmetic due to deterioration of the stability and spreadability of the formulation.
상기 발효물은 DPPH 소거 활성, 콜라겐 생성, 콜라게나아제 억제 효능, 멜라닌 생성 저해 효능 및 히알루론산 생성 효능을 갖는 것을 특징으로 한다. 즉, 항산화, 주름개선, 미백 및 보습 효과를 동시에 부여할 수 있기 때문에 단순히 피부 미용 뿐만 아니라, 근본적인 피부 건강 개선을 구현할 수 있는 효과가 있다. The fermentation product is characterized by having DPPH scavenging activity, collagen production, collagenase inhibitory effect, melanin production inhibitory effect, and hyaluronic acid production effect. That is, since it can provide antioxidant, wrinkle improvement, whitening and moisturizing effects at the same time, there is an effect that can realize not only skin beauty but also fundamental skin health improvement.
전술된 바와 같이, 발효물은 항산화, 주름개선, 미백 및 보습 효능이 우수하기 때문에, 항노화 화장료 조성물로 사용될 수 있다. As described above, since the fermented product has excellent antioxidant, wrinkle improvement, whitening and moisturizing effects, it can be used as an anti-aging cosmetic composition.
본 발명의 일 구현예에 따르면, 상기 화장료 조성물로 사용할 경우, 피부외용연고, 크림, 유연화장수, 수렴화장수, 영양화장수, 팩, 에센스, 헤어토닉, 샴푸, 린스, 헤어 컨디셔너, 헤어 트리트먼트, 스프레이, 젤, 스킨로션, 스킨소프너, 스킨토너, 아스트린젠트, 로션, 밀크로션, 모이스처 로션, 영양로션, 마사지크림, 영양크림, 아이크림, 모이스처 크림, 핸드크림, 파운데이션, 영양에센스, 선스크린, 비누, 클렌징폼, 클렌징로션, 클렌징크림, 클렌징워터, 파우더, 바디로션 및 바디 클렌저로 이루어진 군으로부터 선택된 어느 하나의 제형을 가질 수 있으나, 이에 제한되지 않는다. 이들 각 제형의 조성물은 그 제형의 제제화에 필요하고 적절한 각종의 기제와 첨가물을 함유할 수 있으며, 이들 성분의 종류와 양은 당업자에 의해 용이하게 선정될 수 있다.According to one embodiment of the present invention, when used as the cosmetic composition, skin ointment, cream, soft lotion, astringent lotion, nutrient lotion, pack, essence, hair tonic, shampoo, conditioner, hair conditioner, hair treatment, spray , Gel, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, eye cream, moisture cream, hand cream, foundation, nutrition essence, sunscreen, soap, It may have any one formulation selected from the group consisting of cleansing foam, cleansing lotion, cleansing cream, cleansing water, powder, body lotion, and body cleanser, but is not limited thereto. The composition of each of these formulations may contain various bases and additives necessary and appropriate for formulation of the formulation, and the types and amounts of these components can be easily selected by those skilled in the art.
본 발명의 제형이 페이스트, 크림 또는 겔인 경우에는 담체 성분으로서 동물성 기름, 식물성 기름, 왁스, 파라핀, 전분, 셀룰로오스 유도체, 폴리에틸렌글리콜, 실리콘, 벤토나이트, 실리카, 탈크 또는 산화아연, 산화 티타늄 등이 이용될 수 있다.When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, titanium oxide, etc. may be used as carrier components. I can.
본 발명의 제형이 바디젤인 경우에 전술한 유효성분들 이외에 일반적으로 바디젤 제조 시에 사용되는 성분인, 카보머, 트리에탄올아민, 멘톨, 에탄올, 글리세린, 디소디움이디티에이, 메틸파라벤, 향 등이 이용될 수 있다. 여기서, 카보머, 트리에탈올아민은 점증제로 작용하고, 멘톨은 향 성분으로 작용하며, 에탄올은 청량감을 부여하는 기능을 하고, 글리세린은 보습제로서의 역할을 하며, 디소디움이디티에이는 금속성분 킬레이트로 기능하고, 메틸파라벤은 방부제로의 기능을 위해 첨가된다. 또한, 상기 향은 멘톨향 이외에도 허브향 등과 같은 다양한 향이 첨가될 수 있다. When the formulation of the present invention is a body gel, in addition to the above-described active ingredients, ingredients generally used in the manufacture of body gels, such as carbomer, triethanolamine, menthol, ethanol, glycerin, disodium EDTA, methylparaben, fragrance, etc. Can be used. Here, carbomer and triethanolamine act as a thickening agent, menthol acts as a fragrance ingredient, ethanol acts as a refreshing sensation, glycerin acts as a moisturizer, and disodium EDTA is a metal chelate. It functions as, and methylparaben is added to function as a preservative. In addition, in addition to menthol fragrance, various fragrances such as herbal fragrance may be added to the fragrance.
전술한 구체적인 성분 이외에도, 상기에서 설명한 점증제, 청량감 부여, 향 성분, 보습제 등의 기능을 달성하기 위해 본 기술 분야에서 널리 사용되고 있는 공지의 물질들을 적용하여 조성물을 제조하는 것 또한 본 발명의 범위에 포함될 수 있다.In addition to the above-described specific ingredients, it is also within the scope of the present invention to apply known substances widely used in the present technical field to achieve the functions of a thickener, a refreshing sensation, a fragrance ingredient, a moisturizer, and the like described above. Can be included.
본 발명의 제형이 파우더 또는 스프레이(분무제)인 경우에 담체 성분으로서 락토스, 탤크, 실리카, 알루미늄 히드록시드, 칼슘 실리케이트 또는 폴리아미드 파우더가 첨가될 수 있고, 특히 스프레이인 경우에는 추가적으로 클로로플루오로히드로카본, 프로판/부탄 또는 디메틸에테르와 같은 추진체를 포함할 수 있다.When the formulation of the present invention is a powder or spray (atomizing agent), lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be added as a carrier component. Propellants such as carbon, propane/butane or dimethyl ether may be included.
본 발명의 제형이 용액 또는 유탁액인 경우에는 담체 성분으로 용매, 용해화제 또는 유탁화제가 첨가되는데, 이의 예로는 물, 에탄올, 이소프로판올, 에틸 카보네이트, 에틸 아세테이트, 벤질 알코올, 벤질 벤조에이트, 프로필렌글리콜, 1,3-부틸글리콜 오일, 글리세롤 지방족 에스테르, 폴리에틸렌글리콜, 소르비탄의 지방산 에스테르 등이 있다.When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is added as a carrier component, examples of which include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol , 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, fatty acid ester of sorbitan, and the like.
본 발명의 제형이 현탁액인 경우에 담체 성분으로서 물, 에탄올 또는 프로필렌글리콜과 같은 액상의 희석제, 에톡실화이소스테아릴 알코올, 폴리옥시에틸렌 소르비톨 에스테르 및 폴리옥시에틸렌 소르비탄 에스테르와 같은 현탁제, 미소결정성 셀룰로오스, 알루미늄 메타히드록시드, 벤토나이트, 아가 또는 트라칸트 등이 이용될 수 있다.When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, crystallites Sex cellulose, aluminum metahydroxide, bentonite, agar or tracanth, and the like can be used.
본 발명의 제형이 계면활성제 함유 클린징인 경우에는 담체 성분으로서 지방족 알코올 설페이트, 지방족 알코올 에테르설페이트, 설포숙신산 모노에스테르, 이세티오네이트, 이미다졸리늄 유도체, 메틸타우레이트, 사르코시네이트, 지방산 아미드 에테르 설페이트, 알킬아미도베타인, 지방족 알코올, 지방산 글리세리드, 지방산 디에탄올아미드, 식물성 유, 라놀린유도체 또는 에톡실화 글리세롤 지방산 에스테르 등이 이용될 수 있다.When the formulation of the present invention is a surfactant containing cleansing, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether Sulfate, alkylamidobetaine, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters may be used.
본 발명의 일 구현예에 따르면, 항노화 화장료 조성물은 통상의 첨가제를 부가하여 지방 분해 촉진을 위한 바디젤, 바디 로션, 바디 크림, 바디 오일 등의 조성물로 제조될 수 있으며, 에어졸 타입으로도 제조될 수 있다. 이 경우에 화장료 조성물은 피부에 직접 도포하거나 살포하는 경피 투여의 방법으로 사용되는 것이 바람직하다.According to one embodiment of the present invention, the anti-aging cosmetic composition may be prepared as a composition such as body gel, body lotion, body cream, body oil, etc. for promoting fat decomposition by adding conventional additives, and also manufactured in an aerosol type. Can be. In this case, the cosmetic composition is preferably used as a method of transdermal administration by directly applying or spraying on the skin.
본 발명의 일 구현예에 따르면, 항노화 화장료 조성물의 사용량은 연령, 피부 지방의 상태 정도 등의 개인차나 제형, 형태에 따라 적절하게 조절될 수 있으며, 항산화, 주름개선, 미백 및 보습 효과를 위한 조성물로 유용하게 사용될 수 있다.According to one embodiment of the present invention, the amount of the anti-aging cosmetic composition may be appropriately adjusted according to individual differences such as age and skin fat condition, formulation, and shape, and for antioxidant, wrinkle improvement, whitening and moisturizing effects. It can be usefully used as a composition.
상기와 같은 방법으로부터 수득된 조성물은 DPPH 소거 활성, 콜라겐 생성, 콜라게나아제 억제 효능, 멜라닌 생성 저해 효능 및 히알루론산 생성 효능을 나타내어 우수한 항산화, 주름개선, 미백 및 보습용 화장품을 제공할 수 있다.The composition obtained from the above method exhibits DPPH scavenging activity, collagen production, collagenase inhibitory effect, melanin production inhibitory effect, and hyaluronic acid production effect, thereby providing excellent antioxidant, wrinkle improvement, whitening and moisturizing cosmetics.
또한, 본 발명의 일 구현예에 따르면, 본 발명은 오디 및 설탕을 항아리에 넣고 혼합하여 1차 발효시키는 단계; 상기 1차 발효된 오디 발효물을 여과하여 발효오디청 및 발효오디박을 각각 분리하는 단계; 상기 발효오디청과 도인(Prunus persica (Linn) Batsch), 목단피(Paeomia suffruticosa), 향부자(Cyperus rotundus) 및 후박(Magnolia officinalis)의 식물 복합 추출물을 항아리에 넣고 혼합한 다음 정제수를 첨가하는 단계; 및 상기 발효오디박 및 정제수를 항아리에 넣어 혼합한 다음, 천으로 항아리를 씌운 후 2차 발효시키는 단계를 포함하는 것을 특징으로 하는 식물 복합 발효물을 유효성분으로 포함하는 항노화 화장료 조성물의 제조방법에 관한 것이다.In addition, according to an embodiment of the present invention, the present invention comprises the steps of first fermenting by mixing mulberry and sugar in a jar; Filtering the first fermented mulberry fermented product to separate the fermented mulberry and the fermented mulberry, respectively; Putting and mixing the plant complex extracts of the fermented Audicheong and Doin ( Prunus persica (Linn) Batsch ), Mokdanpi (Paeomia suffruticosa), Hyangbuja (Cyperus rotundus ), and Magnolia officinalis in a jar, and then adding purified water; And mixing the fermented mulberry and purified water in a jar, covering the jar with a cloth, and performing secondary fermentation. It is about.
본 발명의 일 구현예에 따르면, 상기 1차 발효는 오디 및 설탕을 1:1~2의 비율로 항아리에 넣고 12 내지 36 시간 후, 하루에 한번씩 저어주면서 15 내지 25℃에서 7 내지 42일 동안 발효 및 숙성시키는 것일 수 있다.According to an embodiment of the present invention, in the first fermentation, mulberry and sugar are put into a jar in a ratio of 1:1 to 2 and after 12 to 36 hours, stirring once a day for 7 to 42 days at 15 to 25°C. It may be fermented and aged.
이때, 오디 및 설탕의 비율이 상기 범위를 벗어날 경우에는 발효 효율이 낮아지거나 과량의 당이 잔존할 수 있는 문제점이 있기 때문에 바람직하지 않다.At this time, when the ratio of the mulberry and sugar is out of the above range, the fermentation efficiency is lowered or there is a problem in that an excessive amount of sugar may remain, which is not preferable.
또한, 1차 발효 및 숙성 시간 및 온도가 상기 범위를 벗어날 경우에는 역시 발효 효율이 낮아지거나 유익균 외의 미생물이 증식할 우려가 있기 때문에 바람직하지 않다. In addition, when the first fermentation and aging time and temperature are out of the above ranges, fermentation efficiency may be lowered or microorganisms other than beneficial bacteria may proliferate, which is not preferable.
상기와 같이 오디를 1차 발효시켜 숙성된 오디 발효물은 여과하여 발효오디청 및 발효오디박으로 각각 분리시킬 수 있다. 이때, 발효오디청은 여과하여 걸러진 액을 의미하고, 발효오디박은 여과하고 남은 부산물을 의미한다.The fermented mulberry fermented by primary fermentation of mulberry as described above may be filtered and separated into fermented mulberry and fermented mulberry. At this time, fermented mulberry cheong refers to the filtered liquid, and fermented mulberry meal refers to the remaining by-products after filtering.
상기 1차 발효 후 숙성된 오디 발효물을 발효오디청 및 발효오디박으로 각각 분리시킨 후 2차 발효를 수행하게 된다. After the first fermentation, the fermented mulberry is separated into fermented mulberry and fermented mulberry, respectively, and then the second fermentation is performed.
2차 발효는 식물 복합 추출물 100 중량부에 대하여 발효오디청 1000 내지 3000 중량부를 항아리에 넣고 혼합한 다음 정제수 4000 내지 2000 중량부를 첨가한 후, 발효오디박 1000 내지 3000 중량부 및 정제수 4000 내지 2000 중량부를 항아리에 넣어 혼합한 다음, 천으로 항아리를 씌운 후 10 내지 25℃에서 7 내지 42일 동안 발효 및 숙성시키는 것일 수 있다.For the secondary fermentation, 1000 to 3000 parts by weight of fermented mulberry extract were put in a jar and mixed, and 4000 to 2000 parts by weight of purified water were added, followed by 1000 to 3000 parts by weight of fermented mulberry and 4000 to 2000 parts by weight of purified water After mixing the poured into a jar, it may be fermented and aged at 10 to 25°C for 7 to 42 days after covering the jar with a cloth.
이때, 상기 식물 복합 추출물은 조성물 총 중량에 대하여 도인(Prunus persica (Linn) Batsch) 10 내지 50중량%, 목단피(Paeomia suffruticosa) 10 내지 50중량%, 향부자(Cyperus rotundus) 10 내지 50중량% 및 후박(Magnolia officinalis) 10 내지 50중량% 포함된 식물 복합물을 용매에 혼합한 다음, 50 내지 60℃에서 1 내지 6시간 동안 추출하여 수득된 것이 DPPH 소거 활성, 콜라겐 생성, 멜라닌 생성 저해 효능 및 히알루론산 생성 효능이 현저하게 증가한다는 측면에서 바람직하다. 만일, 추출 조건이 상기 범위를 벗어날 경우에는 충분한 항산화, 주름개선, 미백 및 보습 효과를 나타낼 수 없기 때문에 바람직하지 않다. At this time, the plant complex extract is doin ( Prunus persica (Linn) Batsch ) 10 to 50% by weight, Mokdanpi (Paeomia suffruticosa ) 10 to 50% by weight, Hyangbuja (Cyperus rotundus ) 10 to 50% by weight and fubak ( Magnolia officinalis ) obtained by mixing a plant complex containing 10 to 50% by weight in a solvent, and then extracting at 50 to 60° C. for 1 to 6 hours, DPPH scavenging activity, collagen production, melanin production inhibitory efficacy and hyaluronic acid production It is preferable in terms of remarkably increasing efficacy. If the extraction conditions are outside the above range, it is not preferable because sufficient antioxidant, wrinkle improvement, whitening and moisturizing effects cannot be exhibited.
또한, 식물 복합 추출물 대비 발효오디청, 발효오디박 및 정제수의 비율이 상기 범위를 벗어날 경우에는 식물 복합 추출물의 발효 효율이 낮아질 수 있기 때문에 바람직하지 않다.In addition, when the ratio of fermented mulberry, fermented mulberry, and purified water to the plant complex extract is out of the above range, the fermentation efficiency of the plant complex extract may be lowered, which is not preferable.
또한, 2차 발효 및 숙성 시간 및 온도가 상기 범위를 벗어날 경우에는 발효 효율이 낮아지거나 유익균 외의 미생물이 증식할 우려가 있기 때문에 바람직하지 않다. In addition, when the secondary fermentation and aging time and temperature are out of the above ranges, fermentation efficiency may be lowered or microorganisms other than beneficial bacteria may proliferate, which is not preferable.
이하, 실시예를 통하여 본 발명을 더욱 상세히 설명 하고자 한다. 이들 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로서, 본 발명의 요지 따라 본 발명의 범위가 이들 실시예에 의해 제한되지 않는다는 것은 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자에게 있어서 자명할 것이다. Hereinafter, the present invention will be described in more detail through examples. These examples are only for describing the present invention in more detail, and that the scope of the present invention is not limited by these examples according to the gist of the present invention for those of ordinary skill in the art to which the present invention pertains. It will be self-evident.
<실시예 1> 식물 복합 발효물 제조<Example 1> Preparation of plant complex fermented product
도인(지유본초), 목단피(지유본초), 향부자(지유본초) 및 후박(지유본초)를 각각 1:1:1:1:1로 혼합한 다음, 혼합물 100g에 75%(v/v) 에탄올 수용액을 1L 첨가하여 60℃에서 3시간 동안 가온 추출하였다. 추출한 다음, 여과지로 여과하고 상기 추출 공정을 2회 반복하여 실시한 후 감압 농축하여 분말화된 식물 복합 추출물을 제조하였다.Doin (Jiyu herbal), Mokdanpi (Jiyu herbal), Hyangbuja (Jiyu herbal) and Hubak (Jiyu herbal) are mixed at 1:1:1:1:1, respectively, and then 75% (v/v) ethanol in 100 g of the mixture 1 L of aqueous solution was added and extraction was performed by heating at 60° C. for 3 hours. After extraction, filtration through filter paper and repeating the extraction process twice, and then concentrated under reduced pressure to prepare a powdered plant complex extract.
오디와 설탕을 1:1로 항아리에 번갈아 넣고 2일 후 하루 한 번씩 저어주면서 2주간 상온(20~25℃)에서 발효 및 숙성시켰다. 숙성된 오디를 여과하여 발효오디청 및 발효오디박을 각각 분리하여 1차 발효시켰다.Mulberry and sugar were alternately placed in a jar at 1:1, and after 2 days, they were fermented and aged at room temperature (20~25℃) for 2 weeks while stirring once a day. The aged mulberry was filtered to separate fermented mulberry and fermented mulberry, respectively, and subjected to primary fermentation.
발효오디청 2kg에 상기 분말화된 식물 복합 추출물 100g을 서서히 넣으면서 섞어준 다음, 항아리에 부은 후 정제수 4kg을 첨가하였다. 발효오디박 2kg 및 정제수 2kg을 순차적으로 첨가하여 혼합하고 천으로 항아리를 씌운 후, 하루 한 번씩 저어주면서 20℃ 항온에서 4주간 2차 발효시켰다. 상기 발효물을 No.2 여과지를 이용하여 여과하여 최종 식물 복합 발효물을 제조하였다.100 g of the powdered plant complex extract was slowly added to 2 kg of fermented mulberry, and then mixed, poured into a jar, and then 4 kg of purified water was added. 2 kg of fermented mulberry and 2 kg of purified water were sequentially added and mixed, and a jar was covered with a cloth, followed by secondary fermentation at a constant temperature of 20° C. for 4 weeks while stirring once a day. The fermented product was filtered using No. 2 filter paper to prepare a final plant complex fermented product.
<실시예 2> 식물 복합 발효물의 EA 분획물 제조<Example 2> Preparation of EA fraction of plant complex fermentation
상기 실시예 1에서 수득된 식물 복합 발효물 10g에 증류수를 500mL 첨가한 다음, 에틸아세테이트를 500mL 가한 후 분획 농축하여 최종 식물 복합 발효물의 EA 분획물을 제조하였다.500 mL of distilled water was added to 10 g of the plant complex fermented product obtained in Example 1, 500 mL of ethyl acetate was added, and fractional concentration was performed to prepare an EA fraction of the final plant complex fermentation product.
<비교예 1> 식물 복합 추출물 제조<Comparative Example 1> Preparation of plant complex extract
도인(지유본초), 목단피(지유본초), 향부자(지유본초) 및 후박(지유본초)를 각각 1:1:1:1:1로 혼합한 다음, 혼합물 100g에 75%(v/v) 에탄올 수용액을 1L 첨가하여 60℃에서 3시간 동안 가온 추출하였다. 추출한 다음, 여과지로 여과하고 상기 추출 공정을 2회 반복하여 실시한 후 감압 농축하여 분말화된 식물 복합 추출물을 제조하였다.Doin (Jiyu herbal), Mokdanpi (Jiyu herbal), Hyangbuja (Jiyu herbal) and Hubak (Jiyu herbal) are mixed at 1:1:1:1:1, respectively, and then 75% (v/v) ethanol in 100 g of the mixture 1 L of aqueous solution was added and extraction was performed by heating at 60° C. for 3 hours. After extraction, filtration through filter paper and repeating the extraction process twice, and then concentrated under reduced pressure to prepare a powdered plant complex extract.
<실험예 1> 항산화 시험 : DPPH 소거 활성<Experimental Example 1> Antioxidant test: DPPH scavenging activity
실시예 1~2 및 비교예 1을 이용하여 항산화 효과를 알아보기 위하여 자유라디칼 소거활성을 평가하였다.Free radical scavenging activity was evaluated in order to examine the antioxidant effect using Examples 1 to 2 and Comparative Example 1.
자유라디칼 소거활성 실험은 Kim 등(Kor. J. Pharmacogn., 24(4), 299~303, 1993)의 방법을 변형한 것으로, 안정한 자유라디칼인 DPPH(1,1-diphenyl-2-picryl hydrazyl, Sigma사) 시약을 사용하였다. 먼저, 0.2mM DPPH 용액 1mL에 각각의 실시예 1~2 및 비교예 1을 메탄올에 적당한 농도로 희석하여 2mL 혼합하고, 실온에서 10분간 반응시킨 후 microplate reader(Synergy, BioTek, USA)로 517nm에서 흡광도를 측정하였다. 한편, 자유라디칼 소거시험에서의 대조군은 시료 용액 대신 메탄올을 넣어 같은 방법으로 측정하였으며, DPPH 용액 대신 메탄올을 넣어 각각에 대한 색 보정값을 얻었다. 실험은 각각 3회씩 수행하여 평균값 ± 표준편차로 나타내었으며, 자유라디칼 소거율은 아래의 수학식 1을 이용하여 계산하였다.The free radical scavenging activity experiment was modified from the method of Kim et al. (Kor. J. Pharmacogn., 24(4), 299~303, 1993), and is a stable free radical, DPPH (1,1-diphenyl-2-picryl hydrazyl). , Sigma) reagent was used. First, in 1 mL of 0.2mM DPPH solution, 2 mL of each of Examples 1-2 and Comparative Example 1 was diluted in methanol to an appropriate concentration and mixed with 2 mL, reacted at room temperature for 10 minutes, and then at 517 nm with a microplate reader (Synergy, BioTek, USA). The absorbance was measured. Meanwhile, the control group in the free radical scavenging test was measured in the same way by putting methanol instead of the sample solution, and methanol was added instead of the DPPH solution to obtain a color correction value for each. Each experiment was performed three times and expressed as the mean value ± standard deviation, and the free radical elimination rate was calculated using Equation 1 below.
[수학식 1][Equation 1]
자유라디칼 소거율(%)=100-(각 시료의 흡광도/대조군 흡광도) x 100Free radical scavenging rate (%) = 100-(absorbance of each sample/absorbance of control group) x 100
SC50은 자유라디칼 소거율 50%를 달성하기 위해 소요되는 시료의 농도로서, 다른 성분 물질과 상대적으로 비교할 때 사용하는 수치로 값이 작을수록 소거율이 높음을 나타낸다.SC 50 is the concentration of the sample required to achieve 50% of the free radical scavenging rate, and is a value used when comparing with other component materials. The smaller the value, the higher the scavenging rate.
그 결과, 표 1에서 확인할 수 있듯이 실시예 1~2 및 비교예 1에서 모두 양호한 항산화 효과가 나타났으며, 그 중에서도 실시예 2에서 보다 우수한 항산화 효과가 나타남을 확인하였다. As a result, as can be seen in Table 1, good antioxidant effects were exhibited in both Examples 1 to 2 and Comparative Example 1, and among them, it was confirmed that more excellent antioxidant effects were exhibited in Example 2.
<실험예 2> 주름개선 효능 시험 : 콜라겐 생성 효과 측정<Experimental Example 2> Wrinkle improvement efficacy test: Collagen production effect measurement
실시예 1~2 및 비교예 1을 이용하여 주름개선 효과를 알아보기 위하여 섬유아세포를 이용한 콜라겐 생성 효과를 측정하였다. In order to examine the wrinkle improvement effect using Examples 1 to 2 and Comparative Example 1, the collagen production effect using fibroblasts was measured.
먼저, 배양된 섬유아세포(HS68 cells) 세포를 24-웰 플레이트(24-well plate)에 웰당 1x105 cells/mL로 분주한 후, 24시간 배양하였다. 배양 후, 각각 실시예 1~2 및 비교예 1이 농도별로 희석된 시럼이 함유되지 않은 DMEM 배지로 교체하여 24시간 동안 배양하였다. 콜라겐 합성량은 콜라겐 측정 키트(Procollagen Type I C peptide EIA kit, Takara, Japan)를 이용하여 프로콜라겐(procollagen) 타입 I C-펩타이드(Type I C-peptide, PICP)의 양을 측정하였다. 시료를 처리하지 않은 세포 배양액의 흡광도를 대조군 100%로 하였으며, 실험은 각각 3회씩 수행하여 평균값 ± 표준편차로 나타내었다. First, cultured fibroblasts (HS68 cells) cells were dispensed into a 24-well plate at 1×10 5 cells/mL per well, and then cultured for 24 hours. After cultivation, each of Examples 1 to 2 and Comparative Example 1 was replaced with a DMEM medium containing no syrum diluted by concentration, and cultured for 24 hours. The amount of collagen synthesis was measured by using a collagen measurement kit (Procollagen Type IC peptide EIA kit, Takara, Japan) to measure the amount of procollagen type I C-peptide (PICP). The absorbance of the cell culture solution that was not treated with the sample was set to 100% of the control group, and the experiment was performed three times each and expressed as a mean value ± standard deviation.
그 결과, 표 2에서 확인할 수 있듯이 비교예 1 대비 실시예 1~2이 콜라겐 생성 효과가 우수하여 주름개선 효과가 현저하게 높은 것을 확인하였다.As a result, as can be seen in Table 2, it was confirmed that Examples 1 to 2 compared to Comparative Example 1 had excellent collagen production effects, and thus the wrinkle improvement effect was remarkably high.
<실험예 3> 주름개선 효능 시험 : 콜라게나아제(MMP-1) 억제 측정 <Experimental Example 3> Wrinkle improvement efficacy test: collagenase (MMP-1) inhibition measurement
실시예 1~2 및 비교예 1을 이용하여 주름개선 효과를 알아보기 위하여 섬유아세포를 이용한 콜라게나아제(MMP-1) 억제 효능을 평가하였다. In order to examine the wrinkle improvement effect using Examples 1 to 2 and Comparative Example 1, the inhibitory effect of collagenase (MMP-1) using fibroblasts was evaluated.
먼저, 배양된 섬유아세포(HS68 cells) 세포를 24-웰 플레이트(24-well plate)에 웰당 1x105 cells/mL로 분주한 후, 24시간 배양하였다. 배양 후, UV A를 조사하여 콜라게나아제의 생성을 유도하고, 각각 실시예 1~2 및 비교예 1이 농도별로 희석된 시럼이 함유되지 않은 DMEM 배지로 교체하여 48시간 동안 배양하였다. 콜라게나아제 저해 효과는 콜라게나아제 측정 키트(MMP-1, Human, Biotrak ELISA System, GE Healthcare, USA)를 이용하여 콜라게나아제 양을 측정하였다. 시료를 처리하지 않은 세포 배양액의 흡광도를 대조군 100%로 하였으며, 실험은 각각 3회씩 수행하여 평균값 ± 표준편차로 나타내었다. First, cultured fibroblasts (HS68 cells) cells were dispensed into a 24-well plate at 1×10 5 cells/mL per well, and then cultured for 24 hours. After cultivation, UV A was irradiated to induce the production of collagenase, and each of Examples 1 to 2 and Comparative Example 1 was replaced with a DMEM medium containing no syrum diluted by concentration, and cultured for 48 hours. The collagenase inhibitory effect was measured using a collagenase assay kit (MMP-1, Human, Biotrak ELISA System, GE Healthcare, USA). The absorbance of the cell culture solution that was not treated with the sample was set to 100% of the control group, and the experiment was performed three times each and expressed as a mean value ± standard deviation.
그 결과, 표 3에서 확인할 수 있듯이 실시예 2에서 우수한 콜라게나아제 저해 효과가 나타나 주름개선 효과가 현저하게 높은 것을 확인하였다.As a result, as can be seen in Table 3, it was confirmed that the excellent collagenase inhibitory effect was exhibited in Example 2, and the wrinkle improvement effect was remarkably high.
<실험예 4> 미백 효능 시험 : 멜라닌 생성 억제 측정 <Experimental Example 4> Whitening efficacy test: Melanin production inhibition measurement
실시예 1~2 및 비교예 1을 이용하여 미백 효과를 알아보기 위하여 멜라닌 형성 세포를 이용한 멜라닌 생성 억제 효능을 평가하였다. In order to examine the whitening effect using Examples 1 to 2 and Comparative Example 1, the effect of inhibiting melanogenesis using melanocytes was evaluated.
먼저, 배양된 Murine B16F10 melanoma 세포를 6-웰 플레이트(6-well plate)에 웰당 5X104 cells/mL로 분주한 후, 24시간 배양하였다. D-phosphate buffered saline(D-PBS)으로 세척한 다음 0.2μM α-MSH를 포함하는 배지로 교환 후, 각각 실시예 1~2 및 비교예 1을 농도별로 첨가하여 3일간 배양하였다. 3일 동안 배양 후 배지를 제거하고 D-PBS buffer로 세척한 후, 트립신으로 처리하여 세포를 회수하였다. 회수된 세포는 1M NaOH 0.3mL을 참가 후, 55℃에서 1시간 방치하여 세포 내의 멜라닌을 얻었다. 이를 96-웰 플레이트(96-well plate)로 옮긴 후 ELISA reader를 이용하여 490nm에서 흡광도를 측정하였다. 시료를 처리하지 않은 세포 배양액의 흡광도를 대조군 100%로 하였으며, 실험은 각각 3회씩 수행하여 평균값 ± 표준편차로 나타내었다. First, the cultured Murine B16F10 melanoma cells were dispensed into a 6-well plate at 5×10 4 cells/mL per well, and then cultured for 24 hours. After washing with D-phosphate buffered saline (D-PBS) and then exchanged with a medium containing 0.2 μM α-MSH, Examples 1 to 2 and Comparative Example 1 were added for each concentration and cultured for 3 days. After incubation for 3 days, the medium was removed, washed with D-PBS buffer, and then treated with trypsin to recover the cells. The recovered cells were added with 0.3 mL of 1M NaOH, and then allowed to stand at 55° C. for 1 hour to obtain melanin in the cells. After transferring this to a 96-well plate (96-well plate), the absorbance was measured at 490 nm using an ELISA reader. The absorbance of the cell culture solution that was not treated with the sample was set to 100% of the control group, and the experiment was performed three times each and expressed as a mean value ± standard deviation.
그 결과, 표 4에서 확인할 수 있듯이 비교예 1 대비 실시예 1~2이 멜라닌 생성 저해 효과가 우수하였다. 또한, 실시예 1~2 중에서도 실시예 2에서 보다 우수한 멜라닌 생성 저해 효과가 나타나 미백 효과가 현저하게 높은 것을 확인하였다.As a result, as can be seen in Table 4, Examples 1 to 2 compared to Comparative Example 1 had excellent melanin production inhibitory effect. In addition, among Examples 1 to 2, it was confirmed that the melanin production inhibitory effect was more excellent in Example 2, and the whitening effect was remarkably high.
<실험예 5> 보습 효능 시험 : 히알루론산 생성 효과 측정<Experimental Example 5> Moisturizing efficacy test: Hyaluronic acid production effect measurement
실시예 1~2 및 비교예 1을 이용하여 보습 효과를 알아보기 위하여 히알루론산(hyaluronic acid) 생성 효과를 측정하였다.In order to examine the moisturizing effect using Examples 1 to 2 and Comparative Example 1, the effect of generating hyaluronic acid was measured.
먼저, 배양된 HaCaT 세포를 24-웰 플레이트(24-well plate)에 웰당 1x105 cells/mL로 분주한 후, 24시간 배양하였다. 배양 후, 각각 실시예 1~2 및 비교예 1이 농도별로 희석된 시럼이 함유되지 않은 DMEM 배지로 교체하여 24시간 동안 배양하였다. 히알루론산 생성량은 히알루론산 측정 키트(Hyaluronan ELISA Kit, echelon, USA)를 이용하여 히알루론산의 양을 측정하였다. 시료를 처리하지 않은 세포 배양액의 흡광도를 대조군 100%로 하였으며, 실험은 각각 3회씩 수행하여 평균값 ± 표준편차로 나타내었다. First, cultured HaCaT cells were dispensed into a 24-well plate at 1×10 5 cells/mL per well, and then cultured for 24 hours. After cultivation, each of Examples 1 to 2 and Comparative Example 1 was replaced with a DMEM medium containing no syrum diluted by concentration, and cultured for 24 hours. The amount of hyaluronic acid was measured using a hyaluronic acid measurement kit (Hyaluronan ELISA Kit, echelon, USA). The absorbance of the cell culture solution that was not treated with the sample was set to 100% of the control group, and the experiment was performed three times each and expressed as a mean value ± standard deviation.
그 결과, 표 5에서 확인할 수 있듯이 실시예 1~2에서 히알루론산 생성 효과가 나타났으며, 특히, 실시예 1~2 중에서도 실시예 2에서 보다 우수한 히알루론산 생성 저해 효과가 나타나 보습 효과가 현저하게 높은 것을 확인하였다.As a result, as can be seen in Table 5, the hyaluronic acid production effect was exhibited in Examples 1 to 2, and particularly, among Examples 1 to 2, the hyaluronic acid production inhibitory effect was more excellent than that in Example 2, resulting in a remarkable moisturizing effect. It was confirmed that it was high.
이상으로 본 발명 내용의 특정한 부분을 상세히 기술하였는 바, 당업계의 통상의 지식을 가진 자에게 있어서 이러한 구체적 기술은 단지 바람직한 실시 양태일 뿐이며, 이에 의해 본 발명의 범위가 제한되는 것이 아닌 점은 명백할 것이다. 따라서, 본 발명의 실질적인 범위는 첨부된 청구항들과 그것들의 등가물에 의하여 정의된다고 할 것이다.As described above, specific parts of the present invention have been described in detail, and it will be apparent to those of ordinary skill in the art that these specific techniques are only preferred embodiments, and the scope of the present invention is not limited thereby. will be. Accordingly, it will be said that the substantial scope of the present invention is defined by the appended claims and their equivalents.
Claims (10)
Doin ( Prunus persica (Linn) Batsch ), Mokdanpi (Paeomia suffruticosa ), Hyangbuja (Cyperus rotundus ) and Hubak ( Magnolia officinalis ) plant complex fermentation obtained by fermenting the plant complex extract obtained by fermenting with mulberry fermentation as an active ingredient. Anti-aging cosmetic composition.
According to claim 1, wherein the degree the plant combined extracts with respect to the total composition weight (Prunus persica (Linn) Batsch) 10 to 50% by weight, mokdanpi (Paeomia suffruticosa) 10 to 50% by weight, Cyperus rotundus (Cyperus rotundus) 10 to 50 An anti-aging cosmetic composition comprising a plant complex fermentation product, characterized in that it contains 10 to 50% by weight and 10 to 50% by weight and hubak ( Magnolia officinalis) as an active ingredient.
The plant complex fermentation product of claim 1, wherein the extract is extracted with a solvent selected from the group consisting of water, a lower alcohol having 1 to 4 carbon atoms, propylene glycol, glycerin, and a mixed solvent thereof. Anti-aging cosmetic composition comprising.
The anti-aging cosmetic composition according to claim 1, wherein the fermented mulberry is fermented mulberry and fermented mulberry as an active ingredient obtained by fermenting mulberry and sugar in a jar.
The anti-aging cosmetic composition according to claim 1, wherein the plant complex fermentation product contains 0.001 to 20% by weight based on the total weight of the composition.
The method of claim 1, wherein the plant complex fermentation product comprises as an active ingredient a plant complex fermentation characterized in that it has DPPH scavenging activity, collagen production, collagenase inhibitory effect, melanin production inhibitory effect, and hyaluronic acid production effect. Anti-aging cosmetic composition.
상기 1차 발효된 오디 발효물을 여과하여 발효오디청 및 발효오디박을 각각 분리하는 단계;
상기 발효오디청과 도인(Prunus persica (Linn) Batsch), 목단피(Paeomia suffruticosa), 향부자(Cyperus rotundus) 및 후박(Magnolia officinalis)의 식물 복합 추출물을 항아리에 넣고 혼합한 다음 정제수를 첨가하는 단계; 및
상기 발효오디박 및 정제수를 항아리에 넣어 혼합한 다음, 천으로 항아리를 씌운 후 2차 발효시키는 단계를 포함하는 것을 특징으로 하는 식물 복합 발효물을 유효성분으로 포함하는 항노화 화장료 조성물의 제조방법.
Putting mulberry and sugar in a jar and mixing them to perform primary fermentation;
Filtering the first fermented mulberry fermented product to separate the fermented mulberry and the fermented mulberry, respectively;
Putting and mixing the plant complex extracts of the fermented Audicheong and Doin ( Prunus persica (Linn) Batsch ), Mokdanpi (Paeomia suffruticosa), Hyangbuja (Cyperus rotundus ), and Magnolia officinalis in a jar, and then adding purified water; And
A method for producing an anti-aging cosmetic composition comprising a plant complex fermentation as an active ingredient, comprising the step of putting the fermented mulberry and purified water into a jar and mixing, and then covering the jar with a cloth and performing secondary fermentation.
The method of claim 7, wherein the first fermentation is performed by placing mulberry and sugar in a jar at a ratio of 1:1 to 2, and after 12 to 36 hours, fermentation and aging at 15 to 25°C for 7 to 42 days while stirring once a day A method for producing an anti-aging cosmetic composition comprising a plant complex fermentation product as an active ingredient.
The method of claim 7, wherein the plant complex extract is doin ( Prunus persica (Linn) Batsch ) 10 to 50% by weight, Mokdanskin (Paeomia suffruticosa ) 10 to 50% by weight, Cyperus rotundus 10 to 50% by weight, based on the total weight of the composition. A plant complex fermentation product, characterized in that obtained by mixing a plant complex containing 10 to 50% by weight of Magnolia officinalis in a solvent and then extracting at 50 to 60° C. for 1 to 6 hours, as an active ingredient Method for producing an anti-aging cosmetic composition comprising.
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