KR20190005369A - Composition for preventing or improving skin wrinkle comprising c-phycocyanin as effective component - Google Patents

Abstract

The present invention relates to a composition for preventing or ameliorating skin wrinkles comprising C-phycocyanin as an active ingredient. A complex extract of the present invention is without cytotoxicity, protects cells damaged by ultraviolet irradiation, and inhibits expression of MMP-1 remarkably. Thus, a cosmetic composition of the present invention can be useful for cosmetic ingredients for preventing or ameliorating skin wrinkles.

Description

TECHNICAL FIELD The present invention relates to a composition for preventing or improving skin wrinkles containing c-phycocyanin as an active ingredient,

The present invention relates to a composition for preventing or improving skin wrinkles containing cipicocyanin as an active ingredient.

Skin protects the organs in the body from external stimuli and plays an important role in the maintenance of body homeostasis, such as body temperature control. Such skin is aged by various internal and external factors and is divided into endogenous aging by genetic cause and exogenous aging by environmental cause. Among them, photoaging refers to aging by ultraviolet (UV). Recently, the destruction of the ozone layer due to environmental pollution has increased the amount of ultraviolet rays, and accordingly, research on photoaging has been attracting attention (Wang SQ et al., 2010, Dermatol. Ther. 23 (1): 31-47). In the photoaged skin, appearance characteristics such as roughness, loss of elasticity, wrinkles and irregular pigmentation are observed. Among them, the main research field of photoaging is the change of the skin wrinkles. There have been many reports on the basic physiological metabolism changes such as synthesis, degradation, and moisture content of collagen, which is a major constituent of skin, in the formation of skin wrinkles by photoaging (Brenneisen et al., 2002, Ann. NY Acad. Sci., 973: 31-43). Particularly, ultraviolet rays increase the production of reactive oxygen species, collapse of enzymatic and non-enzymatic antioxidant defenses of skin, decrease collagen decomposition and biosynthesis, and decrease collagen in the dermis (Bickers DR , Athar M, 2006, J. Invest. Dermatol., 126 (12): 2565-2575). Collagen degradation enzymes (MMPs) are a major contributor to collagen degradation, which is involved in the degradation of the extracellular matrix and basement membrane. Studies have been reported that the enzyme increases activity by ultraviolet rays and inhibits it, thereby increasing skin thickness and wrinkling induced by ultraviolet light (Inomata S et al., 2003, J. Invest. Dermatol., 120 (1): 128-134). Therefore, it is known that effective control of MMPs is required for prevention and treatment of photoaging.

On the other hand, it is known that c-phycocyanin contains a large amount of blue pigment. C-Pococyanin is water-soluble, has a very strong fluorescence and is highly antioxidant, and has attracted attention as a food, medicine and cosmetic raw material. For example, ciprocyanine has been studied for the purpose of helping the digestion of fat, such as bile pigment, and for immuno-controlling function, antioxidant, anti-cancer, anti-blood coagulation and antiviral effect. It is also known that ciprocycin inhibits the allergic inflammatory response as an inhibitor of the inflammatory COX-2 enzyme and has been studied for the improvement and treatment of allergic diseases such as atopic dermatitis.

As a prior art related to cicapacyin, Korean Patent Laid-Open Publication No. 2017-0036879 discloses a method for extracting and stabilizing phycocyanin for use as a cosmetic raw material, and Korean Patent Laid-Open Publication No. 2015-0143914 discloses a method for extracting spirulina There is disclosed a pharmaceutical composition for preventing and treating obesity containing an active ingredient, but no prior art relating to a composition for preventing or improving skin wrinkles containing the present c-phycocyanin as an active ingredient has been disclosed.

The present invention provides a composition for prevention or improvement of skin wrinkles containing cipicocyanin as an active ingredient and a composition for preventing or improving wrinkles of skin, (c-phycocyanin) has no cytotoxicity, protects cells from damage by ultraviolet irradiation, and significantly inhibits the expression of MMP-1, which is increased by ultraviolet irradiation, thereby completing the present invention.

In order to accomplish the above object, the present invention provides a cosmetic composition for preventing or improving skin wrinkles containing cipicocyanin as an active ingredient.

The present invention also provides a health functional food composition for preventing or improving skin wrinkles containing cipicocyanin as an active ingredient.

The present invention also provides a pharmaceutical composition for preventing or treating skin wrinkles containing cipicocyanin as an active ingredient.

FIELD OF THE INVENTION The present invention relates to a composition for preventing or improving skin wrinkles containing cipicocyanin as an active ingredient. The present invention relates to a composition for protecting or repairing damaged skin cells by irradiation with ultraviolet rays, Gt; MMP-1 < / RTI > Therefore, the cosmetic composition of the present invention can be usefully used in cosmetics for preventing or improving skin wrinkles.

FIG. 1 shows MTS assay results showing the cell survival rate (%) of HaCaT cells according to the treatment of cicapicansin (10, 50, 100, 200 and 250 / / ml) according to the present invention.
FIG. 2 shows the cytoprotective effect of HaCaT cells of the present invention against HaCaT cells (human keratinocyte line) for 24 hours against the cell damage caused by UVB irradiation. (UV Crosslinker, UltR5 Lum) for 30 minutes, and then subjected to an MTS assay. # Indicates that the cell survival rate of the UVB irradiation group was statistically decreased compared with that of the control group, which means that the cell survival rate of the UVB-irradiated group treated with cipicocyanin was lower than that of the UVB irradiation group Which is statistically significant compared to the cell survival rate, which means that p <0.05.
FIG. 3 shows the results of confirming the change in the expression level of MMP-1 according to the cipicocyanin treatment of the present invention. # Indicates that the amount of MMP-1 expression in the UVB-irradiated group was statistically significantly increased compared to that in the control group, which means that p <0.05, and * indicates the amount of MMP-1 expression in the experimental group treated with cipicocyanin and UVB , Which is a statistically significant decrease compared to the amount of MMP-1 expression in the UVB-irradiated group, which means that p <0.05.

The present invention relates to a cosmetic composition for preventing or improving skin wrinkles containing c-phycocyanin as an active ingredient.

The cipicocyanin is preferably derived from microalgae, more preferably from cyanobacteria or red algae, but is not limited thereto.

The cosmetic composition is characterized by inhibiting the expression of MMP-1.

In the cosmetic composition according to one embodiment of the present invention, the cosmetic composition for prevention or improvement of wrinkles of the skin may be used as an external ointment for skin, cream, softening longevity, nutritional lotion, pack, essence, hair tonic, shampoo, rinse, hair conditioner, Lotion, milky lotion, moisturizing lotion, nutrition lotion, massage cream, nutritional cream, moisturizing cream, hand cream, foundation, nutrition essence, sunscreen, soap, skin lotion, skin lotion, skin toner, astringent, lotion, But are not limited to, any one form selected from the group consisting of cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers. The cosmetic composition comprising each of these formulations may contain various bases and additives necessary for formulation of the formulation, and the kinds and amounts of these ingredients can be easily selected by those skilled in the art.

When the formulation of the cosmetic composition of the present invention is a paste, a cream or a gel, an animal fiber, a plant fiber, a wax, a paraffin, a starch, a tragacanth, a cellulose derivative, a polyethylene glycol, a silicone, a bentonite, Etc. may be used.

When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, Propellants such as hydrocarbons, propane-butanes or dimethyl ethers.

When the formulation of the cosmetic composition of the present invention is a solution or an emulsion, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the cosmetic composition of the present invention is an interfacial-active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acethionate, imidazolinium derivative, methyltaurate, sarcosinate , Fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolenic derivative or ethoxylated glycerol fatty acid ester.

The cosmetic composition of the present invention may further contain an excipient including a fluorescent substance, a fungicide, a hygroscopic substance, a moisturizer, a fragrance, a fragrance carrier, a protein, a solubilizer, a sugar derivative, a sunscreen, a vitamin, .

The present invention also relates to a health functional food composition for preventing or improving skin wrinkles containing cipicocyanin as an active ingredient.

The health functional food composition of the present invention can be prepared by directly adding cipicocyanin or mixing with other food or food ingredients, and can be suitably prepared according to a conventional method. Examples of foods that can be added with the above-mentioned cicapycia include dairy products including caramel, meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, ice cream, Beverage, tea, drink, alcoholic beverage, and vitamin complex, and includes all the health functional foods in the conventional sense. That is, there is no particular limitation on the kind of the food. The health functional food composition may contain various nutrients, vitamins, minerals (electrolytes), synthetic and natural flavors, colorants and enhancers (cheese, chocolate etc.), pectic acid and its salts, alkynic acid and its salts, Thickening agents, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. It may also contain flesh for the production of natural fruit juices and vegetable drinks. The above components can be used independently or in combination. In addition, the health functional food composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient. The natural carbohydrates may be selected from the group consisting of glucose, monosaccharides such as fructose, disaccharides such as maltose and sucrose, Polysaccharides such as cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Although the ratio of the natural carbohydrate is not critical, it is preferably 0.01 to 0.04 g, more preferably 0.02 to 0.03 g, per 100 g of the composition of the present invention, but is not limited thereto. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like.

The present invention also provides a pharmaceutical composition for preventing or treating skin wrinkles containing cipicocyanin as an active ingredient.

The pharmaceutical composition of the present invention may further comprise a pharmaceutically acceptable carrier, excipient or diluent. The pharmacologically acceptable carrier to be contained in the pharmaceutical composition of the present invention is one which is usually used at the time of formulation and includes saline, sterilized water, Ringer's solution, buffered saline, dextrose solution, maltodextrin solution, glycerol, ethanol, But are not limited to, sucrose, sucrose, sucrose, sorbitol, mannitol, starch, acacia, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, syrup, methylcellulose, methylhydroxybenzoate, Hydroxybenzoate, talc, magnesium stearate and mineral oil, and the like. In addition, the composition may further contain an antioxidant, a buffer, a bacteriostatic agent, a diluent, a surfactant, a binder, a lubricant, a wetting agent, a sweetener, a flavoring agent, an emulsifier, a suspending agent or a preservative. The pharmaceutical composition of the present invention can be administered orally or parenterally, and parenteral administration can be carried out by injection or application to skin. A suitable dose of the pharmaceutical composition of the present invention may be variously prescribed by factors such as the formulation method, the administration method, the age, body weight, sex, pathological condition, food, administration time, administration route, excretion rate and responsiveness of the patient .

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not limited thereto.

1. Mr. - Preparation of Pycocyanin

The cipicocyanin used in one embodiment of the present invention was purchased from Sigma-Aldrich and used without purification.

2. Cell culture

HaCaT cells were cultured in Dulbecco's Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% And cultured in a medium containing penicillin-streptomycin (PS, Gibco) at 37 ° C and 5% CO ₂ .

Example  One. Cell survival rate  analysis

The cultured HaCaT cells were treated for 24 hours, and the MTS assay (CellTiter Aqueous) was applied to the cultured HaCaT cells by diluting the active ingredient of the present invention, cicapycyanine, at different concentrations (10, 50, 100, 200 and 250 / / One Solution Cell proliferation assay kit was prepared by adding 3- (4,5-dimethylthiazol-2-yl) -5- (3-carboxymethoxyphenyl) -2- (4-sulfophynyl) -2H-tetrazolium, inner salt MTS, Promega Co. Madison , WI, USA), the cell viability was measured at 490 nm using a microplate reader (Molecular Devices, Sunnyvale, Calif., USA).

As a result, the relative cell viability (%) when the sample of the present invention was treated was slightly increased or maintained as shown in Fig. Therefore, it was found that the present c-phycocyanin had little cytotoxicity.

Example  2. Confirmation of cytoprotective effect by ultraviolet irradiation

After incubation for 30 min with ultraviolet light (UV Crosslinker, Ultra Lum), HaCaT cells were treated with dilution of each sample for 24 hours. Cells were stained with CellTiter Aqueous One Solution Cell proliferation assay kit, 3- (4,5- The cell viability was measured at 490 nm using a microplate reader (MTS, Promega Co., Madison, Wis., USA) Reader (Molecular Devices, Sunnyvale, Calif., USA).

As a result, it was confirmed that the cell viability (%) was reduced by about 50% by UVB irradiation as shown in FIG. 2, and the cell survival rate (%) after treatment with UV- ) Was increased in a concentration-dependent manner, indicating that the cell-protective effect of the present invention was demonstrated.

Example  3. MMP -1 expression level

Each sample was treated with HaCaT cells for 24 hours and irradiated with ultraviolet light (UV Crosslinker, Ultra Lum) at 20 mJ / cm ^{2. Then} , using an MMP-1 ELISA kit (R & D Systems, Inc., Minneapolis, The expression level of MMP-1 was measured.

Specifically, HaCaT cells were plated on a 96-well plate, and then the seed-phycocyanin of the present invention was treated with concentrations (10, 50, 100 and 200 μg / ml), respectively. Then, ultraviolet (UV) irradiation was performed, and the supernatant of the culture was collected and centrifuged at 13,000 rpm for 5 minutes. The supernatant was then subjected to fluorescent assay using an ELISA kit to quantitatively analyze the expression level of MMP-1.

As a result, it was confirmed that the expression level of MMP-1 was remarkably increased in the group irradiated with ultraviolet light, as compared with the negative control group not irradiated with ultraviolet light as shown in Fig. 3. In the group treated with cipicocyanine of the present invention , The expression level of MMP-1 was significantly decreased in a concentration-dependent manner as compared with the ultraviolet irradiation group.

Claims (6)

A cosmetic composition for preventing or improving skin wrinkles containing c-phycocyanin as an active ingredient. The cosmetic composition according to claim 1, wherein the seed-phycocyanin is derived from microalgae. The cosmetic composition according to claim 1, wherein the cosmetic composition inhibits the expression of MMP-1. The cosmetic composition according to claim 1, wherein the cosmetic composition is at least one selected from the group consisting of an ointment for external use, a cream, a softening agent, a nutritional lotion, a pack, an essence, a hair tonic, a shampoo, a rinse, a hair conditioner, a hair treatment, a gel, a skin lotion, Lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutritional cream, moisturizing cream, hand cream, foundation, nutrition essence, sunscreen, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser Wherein the composition is at least one selected from the group consisting of: A health functional food composition for preventing or improving skin wrinkles containing c-phycocyanin as an active ingredient. A pharmaceutical composition for preventing or treating skin wrinkles containing c-phycocyanin as an active ingredient.

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