KR20170130159A - Composition comprising water-soluble propolis composition for preventing or treating acne - Google Patents

Abstract

The present invention relates to a composition for preventing or treating acne which comprises water-soluble propolis containing a propolis solution and honey, and to a cosmetic composition for preventing or treating acne, wherein the composition can easily be used as various pharmaceutical compositions, skin external compositions, and cosmetic materials by simply producing the water-soluble propolis and dissolving the same in water.

Description

[0001] The present invention relates to a composition for preventing or treating acne containing water-soluble propolis,

The present invention relates to a composition for preventing or treating acne characterized by containing a water-soluble propolis mixed with a propolis solution and honey. More particularly, the present invention relates to a propolis solution obtained by dissolving a propolis raw material which does not dissolve in water in an aqueous 50 to 90% (v / v) ethanol solution, 5 to 20 g of honey per 1 ml of the propolis solution And to a composition for preventing or treating acne containing water-soluble propolis obtained by mixing.

The causes of acne are still not clearly defined, but if genetic and environmental factors are excluded, three important causes are directly related to the occurrence of acne. have. The three most important causes of acne are described below. The first cause is excessive sebum secretion. The formation of sebum is accomplished by the action of androgenic hormones. Through the abnormal increase of this male hormone or various related mechanisms, the sebaceous glands become hyperfunction and result in excess sebum. The second is explained by the closure of the pores due to hyperkeratinization of the hair follicles. The resulting sebum must be smoothly drained out of the skin, but due to the overgrowth of the hair follicles, the pores are clogged and smooth release of the sebum is suppressed, resulting in stagnation of sebum in the hair follicle resulting in formation of fine sebaceous glands. In association with sebaceous glands, there is all the enzymes necessary to convert an inactive steroid precursor into active androgen in the skin, and 5α-reductase is one of them. Testosterone, one of the male hormones, enters the sebaceous gland and is converted to dihydrotestosterone by the 5α-reductase present in the cell (sebocyte). It is used as a signal to express a multistep pathway such as gene expression And as a result, sebum is produced. The third cause is caused by skin irritants such as Staphylococcus epidermis , Staphylococcus aureus , and Propionibacterium acnes , which cause acne, and this causes acne lesion worsening As a major cause of The acne bacteria that reside in the hair follicles form pore wall irritants. As the excess sebum secretion continues, the size of the fine cottonseed is gradually increased and the cottonseed is developed. As a result, the hair follicle wall becomes thinner, and the irritant material produced by the acne bacteria stimulates the thinned hair follicle wall, (Papule → pustule → nodule → scar). Various studies have been conducted on methods for treating or improving acne by focusing on sebum suppression and antimicrobial effects against skin diseases according to the causes of such acne.

Propolis is also known as a bag, and it is made by adding bee saliva to the substance secreted by the bark of a tree or other fruit juice, . Such propolis has a characteristic of developing unique fragrance inherent to itself, and in recent years, application studies of propolis have been actively carried out in recent years, and antibiotic effect, antifungal effect and antiviral effect have been revealed, (Bronchial asthma, drug allergy), arteriosclerosis, hypertension, prostate, thyroid dysfunction, gynecological disease, rheumatism, cerebrovascular insufficiency, mental anxiety, dental disease Diseases), skin diseases, and the like. It is also known that propolis can be applied to irritated skin against common drugs as well as the effects of rough skin, nutrition, allergic diseases and acne treatment when applied to the skin. However, propolis is a useful substance containing various vitamins, minerals, and various enzymes. On the other hand, in the state of solids, solubility in water is very low, and when it is mixed with water, phase separation occurs and a solid mass is formed. It is said that it is difficult to develop various products because it sticks and becomes rough. Many researches have been carried out to solve this problem. Korean prior art 10-1995-0002774 (soluble propolis composition), Korean Patent No. 10-0478021 (propolis extract with improved water solubility) And the like are known. However, the above-described prior arts have a complicated manufacturing process or require the addition of a separate food additive, which makes it difficult to harmlessly use the human body.

In order to solve such problems, the present inventors have completed the present invention by preparing a water-soluble propolis having an excellent effect of preventing, treating and improving acne by mixing a propolis mixture obtained by dissolving in an aqueous ethanol solution with honey.

Korean Patent Publication No. 10-1995-0002774 (title of the invention: propolis composition for solubilization, applicant: Yuhan Co., Ltd., published on March 12, 1994) Korean Patent No. 10-0478021 (title of the invention: propolis extract with improved water solubility, applicant: Hayashibara Seibutsu Chemical Co., Ltd .; registered on March 11, 2005) Korean Patent Laid-Open No. 1997-0009768 (title of the invention: cosmetic composition containing propolis, applicant: Park, Jong Sung, published on March 27, 1997) Korean Patent Laid-Open No. 10-2007-0036250 (Title of the invention: Method for producing bio-cellulosic pack containing propolis extract, propolis: Propolis Co., Ltd. Published on April 03, 2007) Korean Patent Laid-Open No. 10-2008-0083596 (Title of invention: A water-soluble propolis-containing beauty mask pack excellent in skin anti-aging and atopic dermatitis and acne treatment, applicant: 18th month)

It is an object of the present invention to provide a composition for preventing or treating acne characterized by containing a water-soluble propolis mixed with a propolis solution and honey. More particularly, the object of the present invention is to provide a propolis solution obtained by dissolving a propolis raw material which is not easily dissolved in water in an aqueous 50 to 90% (v / v) ethanol solution, And a water-soluble propolis obtained by mixing 20 g of the water-soluble propolis.

The present invention relates to a composition for preventing or treating acne containing a water-soluble propolis mixed with a propolis solution and honey.

The water-soluble propolis may be mixed with 5 to 20 g of honey per 1 ml of the propolis solution.

The propolis solution may be obtained by dissolving the propolis raw material in 50 to 90% (v / v) aqueous ethanol solution. At this time, it may be dissolved in 2 to 5 L of an aqueous 50 to 90% (v / v) ethanol solution per kg of the propolis raw material.

The honey can be used as an emulsifier of the propolis solution.

The water-soluble propolis may be added to at least one skin topical bacterium selected from the group consisting of Staphylococcus epidermis , Staphylococcus aureus , and Propionibacterium acnes . And has an antibacterial effect.

The present invention also relates to a cosmetic composition for preventing or improving acne containing water-soluble propolis in which the propolis solution and honey are mixed. Accordingly, the present invention can provide a cosmetic composition containing the cosmetic composition as a raw material or an effective ingredient. Examples of the cosmetics include ointments, essences, whitening creams, lotions, emulsions, packs, general lotions, skin milks, skins, creams, serum, cosmetic soaps, softening lotions, medicated lotions, body cleansers, cleansing oils, cleansing creams, Tissue < / RTI > and cleansing water.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a composition for preventing or treating acne containing a water-soluble propolis mixed with a propolis solution and honey, wherein the water-soluble propolis is prepared by mixing 5 to 20 g of honey per 1 ml of the propolis solution have. When the content of the honey is less than 5 g per 1 ml of the propolis, as shown in FIG. 1, the content of honey is too low to act as an emulsifier, so that the resin-state propolis may not be mixed or the antibacterial activity may be low. If the content of the honey exceeds 20 g per 1 ml of the propolis, the honeycomb does not exhibit a further increased effect, and the solubility may be lowered due to excessive honey content or the antibacterial property may be lowered.

The honey can be used as an emulsifier of the propolis solution. When mixed with water, the propolis solution does not dissolve well in water, and it may appear on the wall of the container or become lumpy. However, when the honey is mixed, the honey acts as an emulsifier, thereby increasing water solubility and providing a propolis which maintains a stabilized aqueous solution state.

The propolis solution may be obtained by dissolving the propolis raw material in 50 to 90% (v / v) aqueous ethanol solution. At this time, if an aqueous solution of ethanol less than 50% (v / v) is used, the solubility of the propolis raw material may be lowered and it may not be suitable for producing the propolis solution. When an ethanol aqueous solution or ethanol solution having a concentration exceeding 90% (v / v) is used, honey may interfere with the function of the emulsifier when the propolis solution and honey are mixed.

The propolis solution may be obtained by dissolving 2 to 5 liters of an aqueous 50 to 90% (v / v) ethanol solution per kg of propolis raw material. If the 50 to 90% (v / v) aqueous ethanol solution is less than 2 L, the propolis solution and the honey may not be mixed well and the honey may not function well as an emulsifier. If the 50 to 90% (v / v) aqueous ethanol solution exceeds 5 L, the antibacterial activity of the water-soluble propolis finally produced may be lowered.

Accordingly, the present invention provides a method for producing a propolis-containing powder, comprising: a first step of dissolving a propolis raw material in 2 to 5 L of an aqueous 50 to 90% (v / v) ethanol solution; A second step of removing the ethanol from the solution in which the propolis raw material is melted to obtain a propolis solution; And a third step of mixing 5 to 20 g of honey as an emulsifying agent per 1 ml of the propolis solution.

The water-soluble propolis may be added to at least one skin topical bacterium selected from the group consisting of Staphylococcus epidermis , Staphylococcus aureus , and Propionibacterium acnes . It has an antibacterial effect and can be usefully used for preventing, improving or treating acne.

The present invention can provide a pharmaceutical composition for preventing or treating acne containing the water-soluble propolis and a pharmaceutical excipient or an external preparation for skin. At this time, the pharmaceutical composition or the external preparation for skin may be administered to mammals such as rats, livestock, and humans in various routes. Preferably a spray, a liquid preparation, a lotion, a foaming agent, a tape agent, an aerosol agent, an external preparation, a gel, a cream, a sol, an ointment agent, a patch agent, a mask pack or the like which is sprayed or applied to the skin. The water-soluble propolis may be contained in the pharmaceutical composition or the external preparation for skin in an amount of 0.001 to 99.9%. The ingredients other than the active ingredient of the pharmaceutical composition or external preparation for skin of the present invention may be any ingredient commonly used in external preparations applied to the skin. For example, bases such as white petrolatum (vaseline), yellow petrolatum, lanolin, purified beeswax, cetanol, stearyl alcohol, stearic acid, hydrogenated oil, hydrocarbon gel, polyethylene glycol, liquid paraffin and squalane; Oleic acid, isopropyl myristate, glycerol triisooctanoate, crotamitone, diethyl sebacate, diisopropyl sebacate, diisopropyl adipate, hexyllaurate, fatty acid, fatty acid ester, vegetable oil, Solvents or stabilizers such as alcohols; Tocopherol derivatives, antioxidants such as L-ascorbic acid, dibutylhydroxytoluene and butylhydroxyanisole; preservatives such as p-hydroxybenzoate; Curling agents such as glycerin, flufolene glycol and sodium hyaluronate; Surfactants such as polyoxyethylene derivatives, glycerol fatty acid esters, sucrose fatty acid esters, sorbitan fatty acid esters, propylene glycol fatty acid esters and lecithin; Thickeners such as carboxyvinyl polymer, xanthan gum, carboxymethylcellulose and sodium carboxymethylcellulose, hydroxypropylcellulose and hydroxypropylmethylcellulose; Stabilizers; Preservatives; Adsorption promoters; And other suitable fillers may be added. The pharmaceutical composition or external preparation for skin of the present invention may be prepared by mixing well the components well known in the art for preparing external preparations, together with a suitable base, if necessary, and the preparation thus prepared is applied to lesions as required .

The dosage of the pharmaceutical composition or external preparation for skin of the present invention will vary depending on the age, sex, body weight of the subject to be treated, and the severity of the pathological condition, the disease or pathological condition, the route of administration and the judgment of the prescriber. Dosage determinations based on these factors are within the level of ordinary skill in the art and generally the dosage ranges from 0.01 mg / kg / day to approximately 2000 mg / kg / day. A more preferable dosage is 1 mg / kg / day to 500 mg / kg / day. The administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way.

However, the formulation of the pharmaceutical composition of the present invention or the skin formulations is most preferably applied in a water-soluble state not containing an oil component, which will be most effective in preventing or improving acne which is active in sebum secretion.

The present invention also relates to a cosmetic composition for preventing or improving acne containing water-soluble propolis in which the propolis solution and honey are mixed. Accordingly, the present invention can provide a cosmetic composition containing the cosmetic composition as a raw material or an effective ingredient. As the cosmetics, The cosmetic composition of the present invention may be formulated into any cosmetic preparation conventionally produced in the art and may be used as an external ointment, essence, whitening cream, lotion, emulsion, pack, general lotion, skin milk, skin, cream, serum, A medicated lotion, a whole body cleanser, a cleansing oil, a cleansing cream, a cleansing tissue and a cleansing water.

The cosmetic preparation of the present invention may contain an ingredient selected from the group consisting of animal oils, vegetable oils, waxes, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, lactose, But are not limited to, calcium silicate, polyamide powder, chlorofluorohydrocarbons, propane / butane or dimethyl ether, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propyleneglycol, Glycerol aliphatic esters, polyethylene glycols or sorbitan fatty acid esters, ethoxylated isostearyl alcohols, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, Aga or Trakant, Province But are not limited to, alcohol sulfates, aliphatic alcohol ether sulfates, sulfosuccinic acid monoesters, isethionates, imidazolinium derivatives, methyltaurate, sarcosinate, fatty acid amide ether sulfates, alkylamidobetaines, fatty acid glycerides, fatty acid diethanolamides, Lanolin derivatives or ethoxylated glycerol fatty acid esters. However, the formulation of the cosmetic composition of the present invention is most preferably applied in a water-soluble state not containing an oil component, which will be most effective in preventing or improving acne which is active in sebum secretion.

Propolis is composed of 50% resin, 30% beeswax, 10% essential oil components such as essential oil, 5% pollen and 5% organic matter and mineral substances. Among the nutrients of propolis, The antimicrobial action of minerals and vitamins, which play an important role in metabolism, is excellent, and the flavonoids, which can be said to be the essence of plants, are extremely high. However, such propolis contains an insoluble component and is not well soluble in water. When dissolved in water, a propolis resin or the like is aggregated, which is disadvantageous in that it is difficult to produce a product without a separate emulsifier. Therefore, conventionally, a method of removing the insoluble component of propolis to make it soluble in water, applying a complicated solubilization step of various stages, or increasing the solubility of propolis by using an expensive additive or the like has been used.

The present invention relates to a composition for preventing or treating acne containing a water-soluble propolis mixed with a propolis solution and honey, and a cosmetic composition for preventing or improving acne, wherein the propolis is dissolved in water It can be easily used in various pharmaceutical compositions, external skin preparations and cosmetic products. In addition, the water-soluble propolis may be used as an antimicrobial agent having excellent antibacterial effect on Staphylococcus epidermis , Staphylococcus aureus , Propionibacterium acnes , By identifying it, it is confirmed that it is effective for prevention, treatment and improvement of acne. In addition, since propolis is excellent in solubility in water, it can be applied as a composition which does not use an emulsifier derived from another oil, so that it is expected that the effect of relieving symptoms of acne which is active sebum secretion is excellent, Is expected to be very high. In particular, since the use of natural cosmetics is rapidly increasing in recent years rather than products containing chemicals or chemical preservatives which are harmful to the skin, when entering into cosmetics market with excellent preservative effect and antibacterial effect without addition of special preservatives or compounds, The product is expected to be competitive.

Korean Patent Laid-Open No. 10-1995-0002774 and Korean Patent No. 10-0478021 disclose a method for solubilizing propolis. However, as described in the present invention, there is no disclosure of a composition in which the water-solubility is improved by mixing honey and propolis not. Although the possibility of using a cosmetic composition using propolis has been disclosed in Korean Patent Publication Nos. 1997-0009768, 10-2007-0036250, and 10-2008-0083596, the present invention And a composition or a cosmetic having the effect of improving the acne using the water-soluble propolis has not been disclosed.

1 is a photograph showing the possibility of producing a water-soluble propolis according to the mixing ratio of propolis solution and honey.
Fig. 2 is a photograph showing the dissolution degree of water-soluble propolis (left) and propolis solution (right) in water.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein but may be embodied in other forms. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the concept of the invention to those skilled in the art.

< Example  1. Preparation of water-soluble propolis>

1 kg of the propolis raw material was dissolved in 3.5 L of an 80% (v / v) aqueous ethanol solution to prepare a propolis solution. The propolis solution was filtered to produce a propolis solution of 36 ° brix. To 25 to 100 ml of the propolis solution, 500 g of various kinds of natural honey were added and mixed to prepare a water-soluble propolis. The propolis solution and honey were mixed and then subjected to mechanical stirring at a constant rate of 100 rpm or less for 30 minutes. At this time, in order to confirm that the mixing ratio of the propolis solution and honey was important for preparing the water-soluble propolis, 500 g of various natural honey was mixed with 150 or 250 ml of the propolis solution.

Experimental Example 1. Confirmation of production of water-soluble propolis [

The propolis solution prepared in Example 1 was allowed to stand at room temperature (one week) and then observed with naked eyes. Referring to FIG. 1, when 25 to 100 ml of propolis is mixed with 500 g of various kinds of natural honey, it is confirmed that both propolis and honey are well mixed and exist in an emulsion state. On the other hand, when 150 ml or 250 ml of propolis was mixed with 300 g of honey, it was confirmed that propolis and honey were not mixed and separated (in FIG. 1, propolis was not mixed in the third and fourth upper layers) . That is, it can be seen that the honey acts as an emulsifier for dissolving the insoluble component in the propolis solution, and it is confirmed that the honey has the function as the emulsifier depending on its content. Therefore, when honey is used as an emulsifier, it is preferable to use 5 to 20 g of honey per 1 ml of the propolis solution.

<Experimental Example 2> Solubility test of water-soluble propolis>

1 kg of the propolis raw material was dissolved in 3.5 L of an 80% (v / v) aqueous ethanol solution to prepare a propolis solution. The propolis solution was filtered to prepare a propolis solution of 36 ° brix. 100 ml of the propolis solution and 1 kg of honey were mixed at 100 rpm or less for 30 minutes using a stirrer to prepare a water-soluble propolis. 5 g of each of the water-soluble propolis or propolis solution was mixed with 100 ml of water and stirred well for 1 minute to 1 hour, and the degree of dissolution was confirmed.

2, it was confirmed that the water-soluble propolis on the left was well dissolved in water to be present in a solution state, while the propolis solution on the right was not soluble in water and an insoluble component separated from water was identified, It can be seen that it fills the wall of the container and does not dissolve well.

<Experimental Example 3> Confirmation of antimicrobial effect of water-soluble propolis>

1 kg of the propolis raw material was dissolved in 3.5 liters of an 80% (v / v) aqueous ethanol solution to prepare a propolis solution. The propolis solution was filtered to prepare a propolis solution of 36 ㅀ brix. 100 ml of the propolis solution and 1 kg of honey were mixed at 100 rpm or less for 30 minutes using a stirrer to prepare a water-soluble propolis.

The antimicrobial activity was confirmed by incubation with Staphylococcus epidermis , Staphylococcus aureus , and Propionibacterium acnes , which are skin-damaging bacteria causing acne, respectively. Respectively. For this purpose, 200 μl of a culture solution obtained by culturing Staphylococcus epidermis, Staphylococcus aureus and Propionibacterium acnes in PDB (potato dextrose broth) at 28 ° C for 5 days was applied to a potato dextrose agar (PDA) plate The same weight of the water-soluble propolis, the propolis solution and the honey sample mixed with each of the honeys was diluted in water, and 50 μl of the diluted solution was applied to a paper disk (diameter: 8 mm) Epidermis, Staphylococcus aureus and Propionibacterium acnes) were placed on a plated plate culture medium and cultured at 28 占 폚 for 7 days in which various pathogenic bacteria were well grown.

Thereafter, the effect of the water-soluble propolis, the propolis solution, and the diluted solution of the honey sample on the respective skin-damaging bacteria was investigated with a low supporting diameter, and the results are shown in Table 1 and Table 2.

Antibacterial target strain Antimicrobial Effect of Water-soluble Propolis Treatment (㎜) Propolis solution +
Nectar Propolis solution +
Citrus honey Propolis Solution + Honey Propolis solution +
Acacia honey Propolis solution +
Honey Staphylococcus epidermis 15.61 15.09 16.58 14.68 14.10 Staphylococcus aureus 11.38 14.82 14.55 13.12 14.48 Propionibacterium  acnes 16.73 18.3 18.54 17.86 18.87

Antibacterial target strain Antibacterial effect of propolis solution or honey treatment alone (㎜) Propolis solution Nectar Citrus honey Honey Acacia honey Honey Staphylococcus epidermis 12.26 - - 9.64 - - Staphylococcus aureus 10.11 - - - - - Propionibacterium acnes 15.69 - - - - -

As can be seen from the above Tables 1 and 2, it can be seen that the treatment with honey alone has little antibacterial effect on the skin uptake bacteria, except that the honey has a slight antibacterial effect. However, it can be confirmed that the water-soluble propolis obtained by mixing the propolis solution and honey has a remarkably increased antimicrobial effect as compared with the propolis solution or honey alone.

The water-soluble propolis has a high solubility in water, and unlike other acne-related products containing oil products, the water-soluble propolis is easy to produce a water-soluble product, and the use of such a water- This can help improve the condition. That is, it can be seen that the water-soluble propolis of the present invention can be effectively used for preventing, treating or improving acne.

&Lt; Preparation example of cosmetic preparation 1. Preparation of mask pack for improving acne >

1 g of the water-soluble propolis prepared in Example 1 was diluted in 100 ml of sterile purified water. The nonwoven fabric exclusively used for the mask pack was sterilized, the nonwoven fabric was impregnated with a water-soluble propolis diluent, and vacuum-packed in a sterile plastic bag.

&Lt; Preparation of cosmetic preparation 2. Preparation of moisturizing agent for acne improvement >

1 g of the water-soluble propolis prepared in Example 1 was diluted in 500 ml of sterilized purified water, added to a concentration of 1% (w / v) of hyaluronic acid, and placed in a sterilized container to prepare an acne moisturizing moisturizer.

Claims (12)

A composition for preventing or treating acne characterized by containing a water-soluble propolis mixed with a propolis solution and honey. The method according to claim 1,
Wherein the water-soluble propolis is a mixture of 5 to 20 g of honey per 1 ml of the propolis solution. 3. The method according to claim 1 or 2,
Wherein the propolis solution is obtained by dissolving the propolis raw material in an aqueous 50 to 90% (v / v) ethanol solution. 3. The method according to claim 1 or 2,
Wherein the honey is used as an emulsifier of a propolis solution. 3. The method according to claim 1 or 2,
The water-soluble propolis may be added to at least one skin topical bacterium selected from the group consisting of Staphylococcus epidermis , Staphylococcus aureus , and Propionibacterium acnes . A composition for preventing or treating acne characterized by having an antibacterial effect. A cosmetic composition for preventing or improving acne characterized by containing a water-soluble propolis mixed with a propolis solution and honey. The method according to claim 6,
Wherein the water-soluble propolis is a mixture of 5 to 20 g of honey per 1 ml of the propolis solution. 8. The method according to claim 6 or 7,
Wherein the propolis solution is obtained by dissolving a propolis raw material in an aqueous 50 to 90% (v / v) ethanol solution. 8. The method according to claim 6 or 7,
Wherein the honey is used as an emulsifier of a propolis solution. 8. The method according to claim 6 or 7,
The water-soluble propolis may be added to at least one skin topical bacterium selected from the group consisting of Staphylococcus epidermis , Staphylococcus aureus , and Propionibacterium acnes . A cosmetic composition for preventing or improving acne characterized by having an antibacterial effect. A cosmetic for preventing or improving acne containing the composition of claim 6 or 7 as a raw material or an active ingredient. 12. The method of claim 11,
The cosmetic may be at least one selected from the group consisting of an ointment for external use on the skin, an essence, a whitening cream, a lotion, an emulsion, a pack, a general lotion, a skin milk, a skin, a cream, a serum, a cosmetic soap, And a cleansing water. The cosmetic for preventing or improving acne.

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