KR20170111769A - Composition containing complex extracts including Tuber magnatum mycelium, Morus bombycis, Agastache rugosa, Rubus hirsutus, and Zizyphus jujube with whitening or antioxidant activity - Google Patents

Abstract

The present invention relates to mycelia of tuber magnatum mycelium, Morus bombycis), Agastache rugosa (Agastache rugosa), jangttalgi (Rubus hirsutus , and a jujube ( Zizyphus jujube ) or a fraction thereof as an active ingredient. The present invention also relates to a cosmetic composition, a food composition, an external composition for external use, a composition for external application for skin, and a pharmaceutical composition.
The compositions of the present invention are excellent in efficiency of inhibiting tyrosinase activity and DPPH radical scavenging activity, and can be used for whitening or antioxidant use.

Description

The present invention relates to a composition for whitening or antioxidation, which comprises a complex extract of a truffle mushroom, a mulberry tree, a bamboo plant, a strawberry tree, and a jujube tree (Composition containing complex extracts including Tuber magnatum mycelium, Morus bombycis, Agastache rugosa, Rubus hirsutus, and Zizyphus jujube with whitening or antioxidant activity}

The present invention relates to a whitening or antioxidative cosmetic composition, a food composition, a quasi-drug composition, an external preparation for skin, and a pharmaceutical composition comprising a complex extract of truffle mushroom, a mulberry tree, a fennel, a strawberry and a jujube, or a fraction thereof as an active ingredient .

The skin color of a person largely depends on the amount of melanin pigment contained mainly in skin cells. People with a lot of melanin pigments have brown or black skin, while those with very low melanin pigment cause vitiligo. Melanin pigment is produced from tyrosine by tyrosinase in pigmented cell called melanocyte which exists in basal membrane of skin. Melanin pigment protects skin from excessive ultraviolet rays, so it plays an important role in suppressing skin damage and skin cancer caused by ducts. However, when melanin is excessively produced by daylight, hormone changes, inflammation, medicines, etc., the pigment of the skin is deposited, which causes cosmetic problems. For example, skin pigmentation of the skin is caused by an increase in the amount of melanin pigment, which means that the production of melanin pigment is promoted or the number of melanocytes is increased.

Therefore, in recent years, various whitening cosmetics and medicines for preventing and improving melanin production have been developed and marketed. Currently, in the development of a whitening agent for skin cosmetics, a method of decolorizing the resulting melanin pigment and a method of inhibiting the activity of tyrosinase, an enzyme that forms a melanin pigment, are known. However, it is known that the whitening agent using tocopherol or the like, which is used for reducing the melanin pigment, has little discoloration effect on the melanin pigment. Accordingly, inhibitors that inhibit the activity of tyrosinase and inhibit the production of melanin pigment have been attracting attention.

Tyrosinase is an enzyme that catalyzes the oxidation of monophenols and is contained in the melanocytes of higher animals. Tyrosine is oxidized by this enzyme and melanin pigment is produced through a series of chemical reactions and oxidation. This melanin pigment is brown or black, which absorbs excess light in vivo, which adversely affects skin beauty. In view of the fact that the activity of tyrosinase is inhibited, the production of melanin pigment can be inhibited. Thus, in the field of the conventional cosmetics, natural plant extracts and the like are used as inhibitors of tyrosinase activity as natural whitening extracts.

On the other hand, the antioxidant function inhibits or slows down the oxidation reaction occurring in the body. Oxidation reaction occurring in the body induces aging and cell transformation to generate cancer cells. Free-radical molecules and reactive oxygen species (ROS) from the mitochondria in the cell respiration process affect nearby cells and damage not only the mitochondria but also the surrounding cells. Therefore, if we can prevent the generation of these free radicals, we can prevent the oxidation of the body. This antioxidant effect is difficult to test free radicals occurring in the human body and is measured using DPPH and ABTS radicals.

The DPPH free radical scavenging activity assay is one of the most commonly used methods for measuring the antioxidative activity of a sample. The phenol compounds, aromatic amines, - 2-picyl-hydrazyl) is a method of measuring the antioxidative activity using the principle that the purple color is changed to yellow. It is possible to measure the antioxidative activity in a relatively short and short time and has a high correlation with the antioxidant activity of the plant This is a widely used method.

Korean Patent Publication No. 10-2016-0021676 (Publication date 2016.02.26.)

One object of the present invention is to provide a tuber magnatum mycelium, Morus bombycis), Agastache rugosa (Agastache rugosa), jangttalgi (Rubus hirsutus ) and a jujube ( Zizyphus jujube ) or a fraction thereof as an active ingredient. The present invention also provides a cosmetic composition for skin whitening or antioxidation.

Another object of the present invention is to provide a method for producing tuber magnatum mycelium, Morus bombycis), Agastache rugosa (Agastache rugosa), jangttalgi (Rubus hirsutus and a jujube ( Zizyphus jujube ) or a fraction thereof as an active ingredient.

Another object of the present invention is to provide a method for producing tuber magnatum mycelium, Morus bombycis , Agastache rugosa , and Rubus hirsutus ) and a jujube ( Zizyphus jujube ) or a fraction thereof as an active ingredient.

Another object of the present invention is to provide a method for producing tuber magnatum mycelium, Morus bombycis), Agastache rugosa (Agastache rugosa), jangttalgi (Rubus hirsutus ) and a jujube ( Zizyphus jujube ) or a fraction thereof as an active ingredient.

Another object of the present invention is to provide a method for producing tuber magnatum mycelium, Morus bombycis), Agastache rugosa (Agastache rugosa), jangttalgi (Rubus hirsutus ) and a jujube ( Zizyphus jujube ) or a fraction thereof as an active ingredient. The present invention also provides a pharmaceutical composition for whitening or antioxidation.

The present inventors have made intensive efforts to develop a plant extract that has superior radical scavenging activity and can effectively inhibit tyrosinase activity than conventional plant extracts. As a result, it has been found that the combined extract of tinea asiatica, mulberry tree, The present inventors have confirmed that they effectively inhibit the activity of cinacalcidase and have excellent radical scavenging ability.

Hereinafter, the present invention will be described in more detail.

In order to accomplish the above object, the present invention provides, as one embodiment, a method of producing tuber magnatum mycelium, Morus bombycis ), red pepper ( Agastache rugosa , and Rubus hirsutus ) and a jujube ( Zizyphus jujube ) or a fraction thereof as an active ingredient.

The cosmetic composition of the present invention is characterized in that the tuber magnatum mycelium, Morus bombycis , Agastache rugosa , and Rubus hirsutus ) and jujube ( Zizyphus jujube ).

The scientific name of the truffle is Tuber magnatum , which is called truffle and white truffle. It is mainly found in the central, northern Italy, and the Italian white truffles are very precious. White truffles are eaten without being cooked. It is sliced thinly over pasta, salad, and egg fritted with butter. It is sliced thinly under the shell of the poultry dish and baked in the oven. It is added when preparing puwagra terrine or stuffing. Of food.

In one embodiment of the present invention, tuber magnatum is cultured in liquid medium to obtain mycelia, and the mycelia are cultured in a large amount through transplantation in a solid medium, followed by drying and pulverization.

The mountain mulberry is a plant belonging to Moraceae (Moraceae), Morus It is a deciduous broad-leaved arboreous tree with a scientific name of bombycis , distributed throughout the country. The growing environment grows in a place where the soil is fertile and watery. Its height is about 7 ~ 8m. Leaves are ovate (ovate) or wide ovate, 2 ~ 22㎝ in length, 1.5 ~ 14㎝ in width, with irregular and sharp sawtooth at the end of leaf. Flowers bloom from under the new branch to bloom. The male flower and the female flower are generally different, but some are rarely together. Fruits ripen from brown to black purple in June and are elliptical, called "loner" and "loss". It is used for ornamental purposes, leaves are edible, fruit and bark are medicinal.

In the present invention, the cosmetic composition of the present invention can be applied to an extract obtained by drying and crushing mountain mulberry, and can be used for extraction, and can contain leaves, stem, stem skin, branches, bark, roots, And may include leaves, stems, stem barks, branches, bark, flowers, or all of them corresponding to the ground part of the mountain mulberry except for the root. More specifically, leaves and stems may be used from above .

In one embodiment of the present invention, the leaves and stem portions of mountain mulberry can be dried and powdered.

It is a perennial herb belonging to the Lamiaceae and belongs to Agastache It has a scientific name of rugosa . Stems grow straight, 10-25 cm high, with spread hairs. Leaves are opposite to each other. Leaves are ovate or wide ovate, 2.5-4cm long, 1.5-2.5cm wide, slightly dull at the end, wide wedge shape, and serrated at the edge. Petiole is 1-2cm long. The flowers bloom in July-August, and they are gathered rhymingly on the axillae in the axillae of the leaves. Bract is shorter than calyx and ovate. Calyx is 3-3.5 cm long, with hairy hairs on the upper part, and downward when lighted, and light brown. Corolla is light purple with no hair. The fruit is round with the findings. It is native to Korea, Gyeongsangnam-do and Jeju-do, and is distributed in Japan.

In the present invention, the cosmetic composition of the present invention can be applied to an extract obtained by drying and crushing a syrup and pulverizing the syrup, and may include leaf, stem, stem skin, branch, bark, root, flower or all of them. Specifically, leaves, stems, stem peels, branches, bark, flowers, or all of them corresponding to the above-ground parts of the bamboo leaves except for roots can be used. In an embodiment of the present invention, the tops containing all of roots, leaves, stems, etc. of the blossoms may be dried and pulverized.

Jangttalgi the tube blank is a deciduous tree belonging to the heavy mimok (Rosales) Rosaceae (Rosaceae), Rubus hirsutus . The growing environment grows in a place where the water is good and sunny. Its height is 20 ~ 60㎝. Leaves are slightly densely hairs on both sides, with sawtooth on the edge, and wing-like leaves composed of 3 ~ 5 small leaves. The stem is thin and standing straight or long side to side, with thorns in place, and new branches have long hair and glandular hair. The flowers bloom one by one with a diameter of 3 ~ 4㎝ at the end of the short branch next to last year's branches. Fruits ripen in July to August and taste good. Fruit is used for food.

In the present invention, the cosmetic composition of the present invention can be applied to an extract obtained by drying and crushing and pulverizing a large-sized staple, and may include a stem, a stem, a stem, a branch skin, a root, a flower, Specifically, it may include a leaf, a stem, a stem skin, a branch, a bark, a flower, or all of them corresponding to the above-ground portion of the strobilum except for the root. More specifically, leaves and stems may be used from above. In one embodiment of the present invention, the leaves and stem portions of the plant can be dried and powdered.

The jujube is a broad-leaved shrub belonging to the family Rizonaceae (Rhamnaceae), Ziziphus, and has a scientific name of Zizyphus jujube . It grows up to 7 ~ 8m in height, and the bark is gray brown. The young branches come out from one place and have a little hair at the end of the branch. Leaves are alternate, ovate, have a sharpness and are round or round. 3 Macs are distinct and there are saw teeth on the edge. At June to July, light green flowers bloom at the end of the branches, bisexual, and 5 ~ 6mm in diameter, forming about 10 blooms. Petals are smaller than calyx pieces, each 5 pieces. The fruit is oval-shaped nucleus with 2 ~ 3cm in length, ripened in green or reddish brown in September-October. Mountain jujube has few fleshy parts and has a lot of thorns on the branches, and the bamboo shoots have less flesh than the jujubes. Wood is used as an instrument, sculpture, interior material, etc., and fruit jujube is vegetated and medicinal. It is a deciduous broad-leaved arboreous tree and its origin is Korea. In China, it was cultivated about 2,500 years ago, and in Korea in the 3rd and 4th centuries, fruit was used for medicine and food. It grows at less than 500m above sea level except for high mountains, and it is distributed in China, southern Europe and Japan. They are resistant to cold and weather, and resistant to air pollution.

In the present invention, the cosmetic composition of the present invention can be applied to an extract obtained by drying and crushing a jujube tree and extracting it, and it can contain leaves, stems, stem barks, branches, bark, roots, And may specifically include leaves, stems, stem bark, branches, bark, flowers or all of them corresponding to the above-ground parts of the jujube except roots, and more specifically, stems may be used from above. In one embodiment of the present invention, the stem portion of the jujube can be dried and pulverized.

In the present invention, the term "above ground" means a portion of the upper surface of the earth about the earth in the plant. That is, it refers to the upper surface of the ground excluding roots, which includes leaves, stems, stem barks, branches, bark, flowers, or all of them except roots.

The term "outpost" in the present invention refers to whole plants such as leaves, stems, stem barks, branches, bark, roots, flowers of plants.

The term "complex extract" in the present invention refers to a form of an extract obtained by mixing natural products such as plants and dried plants, followed by extraction with one or more solvents, or a mixture of extracts obtained by extracting each natural product with a solvent do.

In the present invention, the compound extract is obtained by extracting a mixture of mycelium of tinea asiaticum, mulberry tree, pomegranate jujube, jujube, and jujube with an extraction solvent, or extracting tinea mushroom mycelium extract, mulberry extract, It can be in mixed form.

The term "extract" in the present invention refers to an extract obtained by extracting the extract itself, such as an extract obtained by extraction, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, a controlled preparation or purified product of the extracted solution, , ≪ / RTI > The extract of the present invention can be extracted from natural, hybrid, or mutant plants of the respective plants, and can also be extracted from plant tissue cultures.

In the extraction of the present invention, the extraction method is not particularly limited, and extraction can be carried out according to a method commonly used in the art. Non-limiting examples of the extraction method include hydrothermal extraction, ultrasonic extraction, filtration, and reflux extraction. These may be performed alone or in combination with two or more methods.

Drying of the extract in the present invention can be carried out by a known method insofar as the useful components from the collected plants are not destroyed, and can be carried out, for example, by a natural drying method in shade. In addition, the crushing can be sufficient to break up the crushed to such an extent that the useful components of the plant can be sufficiently extracted in the subsequent extraction process, and can be pulverized. The drying and crushing steps may be carried out in reverse order or repeatedly as required.

In the present invention, tuber magnatum mycelium, Morus bombycis), Agastache rugosa (Agastache rugosa), jangttalgi (Rubus hirsutus ) and jujube ( Zizyphus jujube ) is not particularly limited, and any solvent known in the art can be used. In the present invention, the complex extract can be obtained by extracting with water, C ₁ to C ₄ lower alcohol, alcohol, or a mixed solvent thereof. Further, non-limiting examples of the extraction solvent include water; C ₁ to C ₄ lower alcohols such as methanol, ethanol, propyl alcohol and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or a mixture thereof. Specifically, water, a lower alcohol, 1,3-butylene glycol, and ethyl acetate may be used alone or in admixture of two or more.

In the present invention, tuber magnatum mycelium, Morus bombycis), Agastache rugosa (Agastache rugosa), jangttalgi (Rubus hirsutus ) and jujube ( Zizyphus jujube ), more specifically, ethanol can be used. Using the above solvent, the tuber magnatum mycelium, Morus bombycis ), red pepper ( Agastache rugosa , and Rubus hirsutus ) and jujubes ( Zizyphus jujube ), and then one or more times of extraction to prepare a complex solvent extract. The dry extract obtained by vacuum distillation of the solvent extract, followed by lyophilization or spray drying can be prepared . In a specific embodiment of the present invention, a combined extract of tinea, mulberry, pine bush, strawberry and jujube was prepared by using ethanol as an extraction solvent, which was filtered and concentrated under reduced pressure (Examples 1 and 2) .

The extraction may be carried out by extracting a mixture (complex) comprising the plant-derived dry matter, wherein the tuber magnatum mycelium, Morus bombycis ), red pepper ( Agastache rugosa , Rubus hirsutus , and Zizyphus jujube can be mixed and applied so that the combined extract can have both tyrosinase inhibitory activity and free radical scavenging activity.

In the case of single extracts, the mycelial extract of truffle mushroom and the extract of mulberry tree showed strong tyrosinase inhibitory activity. However, the activity of tyrosinase inhibitory activity is insignificant or insignificant in each of bokbunja extract, jujube extract and jujube extract. On the contrary, DPPH free radical scavenging activity is insignificant in the mycelium extracts of mushroom mycelia and mulberry leaves, respectively.

In the present invention, a complex extract having a strong whitening activity and an antioxidative activity and obtaining an extract from a mixture of the above plants can be prepared in order to prepare a composition having a whitening and antioxidant (anti-aging) complex function. The combined extract may contain 0.1 to 2 parts by weight of mulberry tree and 0.1 to 2.5 parts by weight of the mature mulberry tree based on 1 part by weight of mycelia of truffle mushroom, and in this case, it may have an excellent whitening activity while having antioxidative activity. Particularly, when 0.5 to 1.5 parts by weight of mulberry and 0.5 to 1.5 parts by weight of the mulberry are based on 1 part by weight of the mycelia of truffle mushroom, when the composition is applied singly, And an improved whitening activity can be obtained.

Specifically, the complex extract may contain 0.1 to 2 parts by weight of mulberry tree, 0.1 to 3 parts by weight of a mulberry tree, 0.1 to 3 parts by weight of a whole lotus seed, and 0.1 to 3 parts by weight of a jujube, based on 1 part by weight of the truffle mushroom , More specifically, the combined extract may contain 0.5 to 1.5 parts by weight of mulberry tree, 0.1 to 2.5 parts by weight of the mulberry tree, 0.5 to 1.5 parts by weight of the stearic acid, and 0.1 to 3 parts by weight of the jujube, based on 1 part by weight of the truffle- More specifically, the complex extract contains 0.8 to 1.2 parts by weight of mulberry tree, 0.3 to 0.7 part by weight of a mulberry tree, 0.8 to 1.2 parts by weight of a strawberry, and 0.3 to 0.7 part by weight of a jujube, based on 1 part by weight of the truffle mushroom . In this case, the combined extract having improved whitening and antioxidant activity can be obtained, compared with a single extract, etc., with little cytotoxicity.

The extraction is carried out at a temperature of 70 to 90 DEG C, specifically at an extraction temperature of 80 to 90 DEG C, using a solvent having a volume of 5 to 20 times by volume, specifically 9 to 15 times, To 10 hours, more specifically, for an extraction time of 2 hours to 6 hours. The process of extracting the crude extract of the liquid phase in succession from one to five times, including the above-mentioned drying and crushing, . ≪ / RTI >

In order to remove suspended solid particles, the complex extracts extracted by the present invention may be filtered using filtration, for example, nylon, filter paper, etc., or filtered using freeze filtration or the like, Drying, spray drying, vacuum drying, foam drying, high frequency drying, infrared drying, and the like, but is not limited thereto.

The liquid crude extract may be separated from the dry matter of the plant by vacuum filtration or the like, followed by concentration or drying. For example, the liquid crude extract may be concentrated in a vacuum rotary condenser at 50 to 100 ° C, preferably 80 to 90 ° C, and the liquid extract may be dried to obtain a powdered complex extract have. The concentrated or powdered complex extract may be used in water, alcohol, dimethyl sulfoxide (DMSO), or a mixed solvent thereof, if necessary.

In one embodiment of the present invention, 1 kg of a mixed sample obtained by thoroughly drying a mixture of truffle mushroom mycelium, mulberry sycamore, bamboo shoot, strawberry and jujube at a weight ratio of 2: 2: 1: 2: 1 was added to 9 liters of ethanol, And then the mixture was filtered and concentrated under reduced pressure to obtain a dried complex extract (Example 1).

The term "fraction " as used herein means a product obtained by performing fractionation to separate a specific component or a specific component group from a mixture containing various components.

The fractionation method for obtaining the fraction in the present invention is not particularly limited and may be carried out according to a method commonly used in the art. Non-limiting examples of such a fractionation method include tuber magnatum mycelium, Morus bombycis ), red pepper ( Agastache by treating the compound in a given solvent extract obtained by extracting rugosa), jangttalgi (Rubus hirsutus) and jujube (Zizyphus jujube) and a method of obtaining the fraction from the extract.

The kind of the solvent used for obtaining the fraction in the present invention is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the fraction solvent include polar solvents such as water and alcohol; And non-polar solvents such as hexane, ethyl acetate, chloroform, and dichloromethane. These may be used alone or in combination of two or more. When alcohol is used in the fraction solvent, C ₁ to C ₄ alcohols can be specifically used.

As used herein, the term "whitening" refers to a method of increasing the lightness of skin with reduced brightness due to excessive pigment such as melanin or keeping the brightness of skin at a certain level, Skin, and the like. Specifically, it may mean skin whitening. For the purpose of the present invention, the whitening is carried out by means of inhibiting tyrosinase activity which promotes the synthesis of melanin formed in the skin by the ethanol combined extract of tinea asiaticus, mulberry, But is not particularly limited thereto.

Melanin is a pigment that is widely distributed in living organisms such as skin, hair and eyes. Melanin is synthesized in Melanosome in Melanocyte of human skin. Tyrosine (tyrosine) Melanin is biosynthesized by the oxidation and polymerization reaction which is converted to DOPA (3,4-dihydroxy-phenylalanine) or DOPA quinone (Dopaquinone) as a starting material. Biosynthesized melanin increases resistance to skin irritation such as ultraviolet rays, but since excessive melanin synthesis causes pigmentation such as spots, freckles, and black spots, tyrosinase activity measurement is regarded as an important indicator of skin whitening effect have.

The combined extracts of mycelium of truffle mushroom, mulberry tree, pomegranate juice, jujube, and jujube prepared in Example 1 of the present invention may be 50 times or more as high as the ability to inhibit tyrosinase activity as compared to arbutin, It may be more than double. Specifically, the IC ₅₀ (concentration of a sample inhibiting 50% inhibition of tyrosinase activity) of the combined extract of tinea mushroom mycelium, mulberry tree, sycamore, It was found that the ability to inhibit the activity of tyrosinase was 87 times higher than that of arbutin at 1/87 of arbutin. In addition, it was confirmed that the tyrosinase activity of the combined extracts of mycelium of truffle mushroom, mulberry tree, bamboo shoot, strawberry, and jujube was significantly lower than that of the positive control group, arbutin, at a concentration of 0.8 / / Is significantly high.

In addition, the combined extracts of the mycelia of tinea asiatica, mulberry, pine berry, jujube, and jujube of the present invention showed significantly higher levels of tyrosinase activity, which plays a key role in the melanin biosynthesis process, , Thereby effectively inhibiting the production of melanin pigment, thereby preventing skin aging and whitening effect (Experimental Example 1). That is, the complex extract of the present invention has an excellent inhibitory activity on the activity of tyrosinase, effectively inhibiting the production of melanin, and can be applied to a cosmetic composition for whitening.

The term "antioxidant" used in the present invention means an antioxidant action. The human body has a balance between a prooxidant and an antioxidant, but due to various factors, Becomes unbalanced and tilting toward oxidation promotes oxidative stress leading to potential cellular damage and pathological disease. Reactive oxygen species (ROS), which is a direct cause of oxidative stress, is unstable and highly reactive. It reacts easily with various bio-materials, attacks irreversible damage to cells and tissues, Cytotoxicity and carcinogenesis. Active nitrogen species (RNS) such as NO, HNO ₂ , and ONOO ^- are produced by the immune response of macrophage neutrophils and other immune cells during inflammatory reaction, and ROS is also produced. Such active oxygen oxidizes and destroys cells in the body, thereby exposing them to various diseases. In addition, the antioxidant has a function of inhibiting oxidation of cells due to highly reactive free radicals or reactive oxygen species (ROS) according to oxidative stress caused by intracellular metabolism or ultraviolet rays And eliminating free radicals or reactive oxygen species, thereby reducing damage to the cells.

The tuber magnatum of the present invention mycelium, Morus bombycis), Agastache rugosa (Agastache rugosa), jangttalgi (Rubus hirsutus ) and jujube ( Zizyphus jujube ) have antioxidative activity.

The tine mushroom mycelium prepared in Example 1 of the present invention ( Tuber magnatum mycelium, Morus bombycis , Agastache rugosa , and Rubus IC ₅₀ (concentration of 50% DPPH free radical scavenging sample) value of ethanol extract of hirsutus and Zizyphus jujube was 16.1 ㎍ / ㎖, higher than vitamin C, a positive control group known to have high antioxidant activity . However, DPPH free radical scavenging activity was significantly improved at 100 ㎍ / ㎖ and 20 ㎍ / ㎖ of the combined extracts, and it was confirmed that the concentration of these complex extracts was increased depending on the concentration. Accordingly, the combined extracts of the mycelium of tinea asiatica, mulberry tree, pine berry, and the jujubes of the present invention have excellent antioxidative activity through inhibition of free radicals promoting the skin aging process, and the complex extract of the present invention has antioxidant cosmetic Composition.

In addition, the combined extracts of mycelia of truffle mushroom, mulberry tree, sycamore, jujube, and jujube of the present invention inhibit the free radicals promoting the skin aging process and have excellent antioxidative activity compared with the extracts of truffle mycelium and mulberry Bar, and antioxidant activity, thereby remarkably lowering the degree of aging and skin damage (Experimental Example 2).

In addition, it was confirmed that the combined extracts of mycelia of tinea, mulberry, sycamore, jujube and jujube of the present invention have multiple functions that can be applied simultaneously for excellent whitening and antioxidant use, Experimental Examples 1 and 2).

That is, in the case of each extract alone, the extract of tinea mushroom mycelium and mung bean alone showed an excellent inhibitory activity against tyrosinase, but the DPPH free radical scavenging ability was low. In case of extracts of soleus, The tyrosinase inhibitory activity was low but DPPH free radical scavenging ability was excellent. That is, in the case of each single extract, there was no extract having both tyrosinase inhibitory activity and antioxidant activity. Accordingly, in order to provide a composition having both an antioxidative activity and a whitening effect, the present invention provides a combined extract of tinea mushroom mycelium, mulberry tree, syrup, jujube, and jujube.

In the present invention, the cosmetic composition may be prepared in various forms, for example, emulsion, lotion, cream (oil-in-water type, oil-in-water type, multiphase), solution, suspension (anhydrous and aqueous) , A gel, a mask, a pack or a powder, and the like. The cosmetic composition of the present invention may contain an acceptable carrier in cosmetic formulations in addition to its effective ingredient. As used herein, the term " acceptable carrier for a cosmetic preparation "refers to a compound or composition that is already known and used in the cosmetic preparation, or a compound or composition to be developed in the future, and which has no toxicity such that the human body can be adapted to contact with skin. The carrier may comprise from about 1% to about 99.99% by weight, specifically from about 50% to about 99% by weight of the composition, based on the total weight of the composition.

However, since the ratio depends on the above-mentioned formulation of the cosmetic composition and its specific application site (face or hands) or its preferable application amount, the ratio is limited in any aspect to the scope of the present invention It should not be.

Examples of the carrier include alcohols, oils, surfactants, fatty acids, silicone oils, humectants, moisturizers, viscosifiers, emulsifiers, stabilizers, sunscreens, coloring agents and perfumes. The compounds / compositions which can be used as the carrier and which can be used as alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, moisturizers, viscosifiers, emulsions, stabilizers, sunscreens, A person skilled in the art can select and use appropriate substances / compositions.

Specifically, the cosmetic composition can be manufactured to have a formulation selected from a solution suspension, an emulsion, a gel, a cream, a lotion, a powder, a soap, a cleansing, a body wash, a cleansing and a spray, although the formulation is not limited.

In another aspect of the present invention, there is provided a method of producing a truant mushroom (T uber magnatum mycelium, Morus bombycis , Agastache rugosa , and Rubus hirsutus ) and a jujube ( Zizyphus jujube ) or a fraction thereof as an active ingredient.

In the present invention, the tine mushroom mycelium (T uber magnatum mycelium, Morus bombycis), Agastache rugosa (Agastache rugosa), jangttalgi (Rubus hirsutus ), jujube ( Zizyphus jujube ), complex extract, fraction, whitening and antioxidation are as described above.

The food composition of the present invention may comprise an acceptable food-aid additive and may further comprise suitable carriers, excipients and diluents conventionally used in the manufacture of food.

In the present invention, the term " food " means a natural product or a processed product containing one or more nutrients. Specifically, the term " food " means that the food can be directly eaten through a certain degree of processing. Functional foods, beverages, food additives, and beverage additives. Examples of the food include various foods, beverages, gums, tea, vitamin complex, and functional foods. In addition, the food of the present invention may contain special nutritional foods (eg, crude oil, spirit, infant food, etc.), meat products, fish products, tofu, jelly, noodles (eg, (Such as soy sauce, soybean paste, hot pepper paste, mixed sauce), sauces, confectionery (eg snacks), dairy products (eg fermented milk, cheese), other processed foods, kimchi, pickled foods But are not limited to, natural flavors (eg, ramen soup, etc.), vitamin complexes, alcoholic beverages, alcoholic beverages and other health supplement foods. The functional food, beverage, food additive or beverage additive may be produced by a conventional production method.

The above-mentioned functional food refers to a food group imparted with added value to function or express the function of the food by using physical, biochemical, biotechnological techniques, etc., or to control the biological defense rhythm of the food composition, Means a food which is processed by being designed so as to sufficiently express the body's controlling function against the living body. Specifically, it may be a health functional food. In particular, the composition of the present invention is suitable for application as a food composition for antioxidant or whitening. The functional food may include a food-acceptable food-aid additive, and may further comprise suitable carriers, excipients and diluents conventionally used in the production of functional foods.

In the food composition, the amount of the complex extract may be 0.00001 wt% or more, specifically 0.1 wt% or more, and 80 wt% or less, specifically 50 wt% or less, more specifically 40 wt% or less , And when the food is a beverage, it is contained in a proportion of not less than 0.001 g, specifically not less than 0.01 g, not more than 50 g, specifically not more than 10 g, more specifically not more than 2 g based on 100 ml of the total volume of the food But is not limited thereto.

The food composition of the present invention may contain sweetening agents, flavoring agents, physiologically active ingredients, minerals and the like in addition to the active ingredients thereof. Sweetening agents may be used in an amount that sweetens the food in a suitable manner, and may be natural or synthetic. Specific examples of natural sweeteners include corn syrup solids, sugar sweeteners such as honey, sucrose, fructose, lactose and maltose. Flavors may be used to enhance taste or flavor, both natural and synthetic. Specifically, a natural one is used. When using natural ones, the purpose of nutritional fortification can be performed in addition to the flavor. Examples of natural flavoring agents include those obtained from apples, lemons, citrus fruits, grapes, strawberries, peaches, and the like, or those obtained from green tea leaves, Asiatica, Daegu, Cinnamon, Chrysanthemum leaves and Jasmine. Also, those obtained from ginseng (red ginseng), bamboo shoots, aloe vera, banks and the like can be used. The natural flavoring agent may be a liquid concentrate or a solid form of extract. Synthetic flavors may be used depending on the case, and synthetic flavors such as esters, alcohols, aldehydes, terpenes and the like may be used. Examples of the physiologically active substance include catechins such as catechin, epicatechin, gallocatechin and epigallocatechin, and vitamins such as retinol, ascorbic acid, tocopherol, calciferol, thiamine and riboflavin. As the mineral, calcium, magnesium, chromium, cobalt, copper, fluoride, germanium, iodine, iron, lithium, magnesium, manganese, molybdenum, phosphorus, potassium, selenium, silicon, sodium, sulfur, vanadium and zinc can be used.

In addition, the food composition of the present invention may contain preservatives, emulsifiers, acidifiers, thickeners and the like in addition to the above sweeteners.

Such preservatives, emulsifiers and the like are preferably added in a very small amount as long as they can attain an application to which they are added. The term " trace amount " means, when expressed numerically, in the range of 0.0005% by weight to about 0.5% by weight based on the total weight of the food composition. Examples of the preservative which can be used include calcium sodium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate and EDTA (ethylenediaminetetraacetic acid). Examples of the emulsifier which can be used include acacia gum, carboxymethyl cellulose, xanthan gum, pectin and the like. Examples of the acidulant that can be used include acid, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, and phosphoric acid. Such an acidulant may be added so that the food composition has a proper acidity for the purpose of inhibiting the growth of microorganisms other than the purpose of enhancing the taste. Agents that may be used include suspending agents, sedimentation agents, gel formers, bulking agents and the like.

In another aspect, the present invention relates to a method for producing tuber magnatum mycelium, Morus bombycis , Agastache rugosa , and Rubus hirsutus ) and a jujube ( Zizyphus jujube ) or a fraction thereof as an active ingredient.

In the present invention, the tine mushroom mycelium (T uber magnatum mycelium, Morus bombycis), Agastache rugosa (Agastache rugosa), jangttalgi (Rubus hirsutus ), jujube ( Zizyphus jujube ), complex extract, fraction, whitening and antioxidation are as described above.

The term "quasi-drug product" used in the present invention means products that are less active than drugs, among the products used for diagnosing, treating, improving, alleviating, treating or preventing diseases of human or animal. For example, Quasi-drugs are products that are used for the purpose of treating or preventing diseases of humans or animals, products that are mild or have no direct action on the human body.

 The quasi-drug composition of the present invention can be used as a body cleanser, a disinfectant cleaner, a detergent, a kitchen detergent, a cleaning detergent, a toothpaste, a gauze agent, a wet tissue, a detergent, a soap, a hand wash, a hair cleanser, a hair softener, a humidifier filler, But the present invention is not limited thereto.

In another aspect, the present invention relates to a method for producing tuber magnatum mycelium, Morus bombycis , Agastache rugosa , and Rubus hirsutus ) and a jujube ( Zizyphus jujube ) or a fraction thereof as an active ingredient.

In the present invention, the tine mushroom mycelium (T uber magnatum mycelium, Morus bombycis), Agastache rugosa (Agastache rugosa), jangttalgi (Rubus hirsutus ), jujube ( Zizyphus jujube ), complex extract, fraction, whitening and antioxidation are as described above.

The term "external preparation" of the present invention is a preparation for external use, and includes external preparation, external preparation, external solution, ointment, warning agent, suppository, etc. The external preparation for skin of the present invention, .

The external preparation for skin according to the present invention may be a parenteral dosage form which is formulated in solid, semi-solid or liquid form by adding a compatible inorganic or organic carrier, excipient and diluent. The preparation for parenteral administration may be a transdermal dosage form selected from the group consisting of drops, ointments, lotions, gels, creams, patches, sprays, suspensions, and emulsions.

Examples of the carrier, excipient and diluent which can be contained in the external preparation include lactose, dextrose, sucrose, oligosaccharide, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, Cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

The composition for external application for skin according to each formulation may be mixed with other ingredients other than the complex extract of the present invention without any difficulties by those skilled in the art depending on the formulation or use purpose of other external preparations for skin or the like. Effect can occur.

The composition for external application for skin of the present invention may contain 0.0001 to 30% (w / v) of the combined extract of the total composition by weight. If it is contained at less than 0.0001% (w / v), the antioxidant, whitening effect of the skin can not be substantially expected. On the other hand, if it exceeds 30% (w / v), the overall processability of the composition such as solubility in a solvent may be lowered and its use for various purposes such as formulations may be restricted.

In another aspect, the present invention relates to a method for producing tuber magnatum mycelium, Morus bombycis , Agastache rugosa , and Rubus hirsutus ) and a jujube ( Zizyphus jujube ) or a fraction thereof as an active ingredient. The present invention also provides a pharmaceutical composition for whitening or antioxidation.

In the present invention, the tine mushroom mycelium (T uber magnatum mycelium, Morus bombycis), Agastache rugosa (Agastache rugosa), jangttalgi (Rubus hirsutus ), jujube ( Zizyphus jujube ), complex extract, fraction, whitening and antioxidation are as described above.

In the present invention, the pharmaceutical composition may comprise a pharmaceutically acceptable carrier or an additive. In the present invention, the expression "pharmaceutically acceptable" means that the application (subject) does not have the above-mentioned toxicity that is adaptable without inhibiting the activity of the active ingredient. The "carrier" is defined as a compound that facilitates the addition of a compound into a cell or tissue.

The extracts of the present invention may be administered alone or in admixture with any convenient carrier, etc., and such dosage forms may be single-dose or repeated-dose formulations. The pharmaceutical composition containing the extract may be a solid preparation or a liquid preparation. Solid preparations include, but are not limited to, powders, granules, tablets, capsules, suppositories, and the like. Solid form preparations may include, but are not limited to, carriers, flavoring agents, binders, preservatives, disintegrants, lubricants, fillers, and the like. Examples of the liquid preparation include water, a solution such as a solution of propylene glycol, a suspension, an emulsion, and the like, but not limited thereto, and it can be prepared by adding a suitable coloring agent, a flavoring agent, a stabilizer, a tackifying agent and the like. For example, powders can be prepared by simple mixing of an extract, which is an effective ingredient of the present invention, with a suitable pharmaceutically acceptable carrier such as lactose, starch, microcrystalline cellulose and the like. The granule is the above-mentioned extract of the present invention; A suitable pharmaceutically acceptable carrier; And a suitable pharmaceutically acceptable binder such as polyvinylpyrrolidone and hydroxypropylcellulose, followed by wet granulation using a solvent such as water, ethanol, or isopropanol, or dry granulation using a compressive force . Tablets may also be prepared by mixing the granules with a suitable pharmaceutically acceptable lubricant such as magnesium stearate and then tableting using a tableting machine.

The extract of the present invention may be administered orally or parenterally depending on the disease to be treated and the condition of the subject, an injection (for example, intramuscular injection, intraperitoneal injection, intravenous injection, infusion, subcutaneous injection, implant) Rectal, rectal, transdermal, topical, and the like, but the present invention is not limited thereto. May be formulated into suitable dosage unit formulations, including those conventionally used and non-toxic, pharmaceutically acceptable carriers, additives, vehicles according to the route of administration.

The pharmaceutical composition of the present invention may be administered at a daily dose of from about 0.0001 mg / kg to about 10 g / kg, and from about 0.001 mg / kg to about 1 g / kg. However, the dosage may vary depending on the degree of purification of the mixture, the condition of the patient (age, sex, weight, etc.), severity of the condition being treated, and the like. For convenience, the total daily dose may be administered in divided doses several times a day as needed.

When the composition of the present invention is used as a pharmaceutical composition, the content of the complex extract in the composition can be appropriately adjusted to an effective amount capable of exhibiting anti-inflammatory activity according to the symptoms of the disease, the progress of the symptoms, May be 0.0001% by weight or more, specifically 0.001% by weight or more, and 80% by weight or less, and more specifically, 50% by weight or less based on the total weight of the composition.

The composition of the present invention is composed of mycelium of tuber magnatum mycelium, Morus bombycis), Agastache rugosa (Agastache rugosa), jangttalgi (Rubus hirsutus ) and a jujube ( Zizyphus jujube ) extract or fractions thereof as an active ingredient, has an excellent whitening effect by inhibiting tyrosinase activity, has excellent DPPH radical scavenging ability, and has an antioxidative effect, It is highly utilized. Accordingly, the present invention can be utilized as a cosmetic composition for whitening or antioxidation, a food composition, a quasi-drug composition, a composition for external application for skin, and a pharmaceutical composition.

Fig. 1 shows the results of evaluation of tyrosinase activity of the combined extracts of the truffle mushroom mycelium, the mulberry tree, the pomegranate, the strawberry and the jujube of the present invention.
FIG. 2 shows the DPPH radical scavenging activity of the combined extracts of mycelium of tinea, mulberry, pine needle, jujube, and jujube of the present invention.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily carry out the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

Example  1: Mycelium of truffle mushroom, mountain mulberry, Extreme  And ethanol extract of jujube

1 kg of a mixed sample obtained by mixing completely dried tinea mushroom mycelium, mountain mulberry, pomace, strawberry and jujube at a weight ratio of 2: 2: 1: 2: 1 was added to 9 liters of ethanol, And repeated three times at intervals. The extracted extract was filtered with Watman filter paper (Watts, USA). These filtered plant extracts were concentrated under reduced pressure after removing ethanol at 45 ° C with a vacuum rotary condenser.

Example  2: Mycelium of truffle mushroom, mountain mulberry, Extreme  And 1: 1: 1: 1: 1 ethanol extract of jujube

 1 kg of a mixed sample obtained by mixing completely dried tinea mushroom mycelium, mulberry tree, pomace, strawberry and jujube at a weight ratio of 1: 1: 1: 1: 1 was added to 9 liters of ethanol and extracted, And concentrated under reduced pressure to obtain a combined extract.

Example  3: Mycelium of truffle mushroom, mountain mulberry, Extreme  And 1: 1: 2: 2: 2 ethanol extract of jujube

 1 kg of a mixed sample obtained by mixing completely dried tinea mushroom mycelium, mulberry tree, pomace, strawberry and jujube at a weight ratio of 1: 1: 2: 2: 2 was added to 9 liters of ethanol, , And concentrated under reduced pressure to obtain a composite extract.

Example  4: Mycelium of truffle mushroom, mountain mulberry, Extreme  And 2: 2: 1: 1: 1 ethanol extract of jujube

1 kg of a mixed sample obtained by mixing completely dried tinea mushroom mycelium, mountain mulberry, blossom, strawberry and jujube at a weight ratio of 2: 2: 1: 1: 1 was added to 9 liters of ethanol, , And concentrated under reduced pressure to obtain a composite extract.

Comparative Example  1: Mycelium of truffle mushroom, mountain mulberry, Extreme  Or ethanol extract alone of jujube

1 kg of each of the completely dried, truffle mycelia, mulberry, sycamore, strawberry or jujube was added to 9 L of ethanol and extracted, filtered, and concentrated under reduced pressure to obtain respective extracts.

Experimental Example  One: Comparative Example  One, Example  Inhibitory effect of tyrosinase activity of extracts 1 to 4 (whitening effect)

The dried extracts of the mycelium of truffle mushroom, the mulberry tree, the pomegranate, the jujubu, and the jujube prepared in Comparative Example 1, and the truffle mushroom mycelium prepared in Examples 1 to 4, the mulberry tree, Were assayed for tyrosinase activity.

Tyrosinase, an enzyme purified from potato or mushroom, was obtained from Sigma extracted from mushrooms. First, tyrosine, a substrate, was dissolved in a sodium phosphate buffer solution (0.1 M, pH 6.8) to prepare a solution having a concentration of 0.3 mg / ml. Then, 334 μl of the solution was taken into a test tube and then sodium phosphate buffer solution 0.1M, pH 6.8). The dried extracts of Comparative Example 1 and Examples 1 to 4 were dissolved in methanol to a concentration of 100 mg / ml, diluted with DMSO, and used as an experimental group. Each 2 μl of the diluted extract samples was added to the solution in the test tube and reacted in a 37 ° C thermostat for 10 minutes.

To evaluate the tyrosinase activity of the sample, the substrate, 1M Phosphate Buffer (PB) pH 6.8, was sterilized at 121 ° C for 20 minutes and cooled at room temperature (25 ° C). 1M Phosphate Buffer (PB) pH 6.8 was prepared at 1/15 M (final concentration = 66.6 mM) and L-DOPA was dissolved in phosphate buffer (pH 6.8) to a concentration of 2.0 mM. Tyrosinase was also dissolved in phosphate buffer (pH 6.8), and the concentration was adjusted to 46 units / ml.

In order to evaluate the tyrosinase activity, the samples used for the blank, the control, the single extract of Comparative Example 1, and the combined extracts of Examples 1 to 4 used in the experiment are as follows.

Blank (2 wells): 100 μl DMSO 2 μl, PB 160 μl, not treated

Control (2 wells): 2 μl of 100% DMSO, 120 μl of PB, 40 μl of Tyrosinase

Extract Sample: 2 ㎕ of sample, 120 ㎕ of PB, 40 ㎕ of tyrosinase

The cells were treated with 2 ㎕ of DMSO, 120 ㎕ of PB and 40 ㎕ of tyrosinase with control (2 wells), and 2 ㎕ of extract, 120 ㎕ of PB, and tyrosinase And 40 μl of L-DOPA solution (2 mM) were added to each well. After incubation at 37 ° C for 10 min, OD (absorbance) was measured at 475 nm using a microplate counter. For arbutin used as a positive control, arbutin was dissolved in DMSO to a concentration of 100 mg / ml, diluted again with DMSO, and used in the experiment at 100 μg / ml.

Tyrosinase activity was calculated using the following equation (1), and the results are shown in Table 2 and FIG.

Equation 1

Inhibition (%) = 1- (ControlO.D-SampleO.D) / (ControlO.D-BlankO.D) * 100 (%)

division Tyrosinase  The concentration of the sample inhibiting the activity by 50% (IC ₅₀  : [Mu] g / ml) Comparative Example 1 Mycelium of truffles 18.2 Mountain mulberry 0.8 Fried > 20 Extreme > 20 Jujube > 20 Example 1 Truffle Mycelium: Mountain Mulberry: Bamboo: Strawberry: Jujube
(2: 2: 1: 2: 1) 1.0 Example 2 Truffle Mycelium: Mountain Mulberry: Bamboo: Strawberry: Jujube
(1: 1: 1: 1: 1) 12.6 Example 3 Truffle Mycelium: Mountain Mulberry: Bamboo: Strawberry: Jujube
(1: 1: 2: 2: 2) 15.9 Example 4 Truffle Mycelium: Mountain Mulberry: Bamboo: Strawberry: Jujube
(2: 2: 1: 1: 1) 6.4 Arbutin (positive control) 87

As shown in Table 1, the mycelia extracts of mushroom mycelium and mulberry extract showed strong tyrosinase inhibitory activity, but the tyrosinase inhibitory activity was not shown to be weak or strong in each of the syrup extract, the jujube extract and the jujube extract. In Examples 2 to 4 compared to the complex of the extract of Example truffle mycelium, acid mulberry, Agastache rugosa, ethanol extract of the composite jangttalgi and jujube 1 were tyrosinase inhibitory activity is much higher, and its IC ₅₀ is The ability of tyrosinase to inhibit the activity of tyrosinase was 87 times higher than that of arbutin at 1/87 level of arbutin.

1 is a graph showing the tyrosinase activity (%) of the complex extract prepared in Example 1 of the present invention. As shown in FIG. 1, the extract of ethanol extracts of tinea, mulberry, pine needle, jujube, and jujube showed a significantly lower tyrosinase activity than that of the positive control group at 0.8 μg / ml. In particular, at a concentration of 0.8 μg / ml of the combined extract, tyrosinase activity was markedly lower than that of 100 μg / ml of arbutin, and the efficiency of inhibiting tyrosinase activity was remarkably high.

Experimental Example  2: Comparative Example  One, Example  The extracts 1 to 4 DPPH Scatters  Evaluation (antioxidant activity)

The combined extracts of the mycelium of truffle mushroom prepared in Comparative Example 1, the mulberry tree, the pomegranate, the strawberry or the jujube extract alone, the dried truffle mycelium prepared in Examples 1 to 4, the mulberry tree, Was dissolved in methanol at a concentration of 100 μg / ml to form a sample solution, which was then diluted with DMSO.

100 μl of 5 × 10 ^-4 M DPPH reagent (1-1-diphenyl-2-picryl-hydrazyl) was mixed with each sample solution in a 96-well plate, stirred for 1 minute, reacted in an incubator at 25 ° C. for 30 minutes And the absorbance was measured at 517 nm using a microplate counter. Vitamin C (100 μg / ml) was used as an experimental control, and the experiment was repeated four times.

The absorbance of the single extract of Comparative Example 1, the complex extract of Examples 1 to 4 and the control (Control) was measured to evaluate the DPPH radical scavenging ability. The results are shown in Table 2 and FIG.

In order to evaluate DPPH radical scavenging ability, the sample composition of Blank, control, and experimental group (extract) used in the experiment is as follows.

Blank (2 wells): 100 [mu] l 100% MeOH

Control (2 wells): 100 μl of 100% MeOH, 100 μl of DPPH

Extract Sample: 2 μl of sample, 100 μl of 100% MeOH, 100 μl of DPPH

division DPPH  Concentration of sample with 50% free radical scavenging (IC ₅₀  : [Mu] g / ml) Comparative Example 1 Mycelium of truffles > 20 Mountain mulberry > 20 Fried 14.6 Extreme 10.4 Jujube 13.8 Example 1 Truffle Mycelium: Mountain Mulberry: Bamboo: Strawberry: Jujube
(2: 2: 1: 2: 1) 16.1 Example 2 Truffle Mycelium: Mountain Mulberry: Bamboo: Strawberry: Jujube
(1: 1: 1: 1: 1) > 20 Example 3 Truffle Mycelium: Mountain Mulberry: Bamboo: Strawberry: Jujube
(1: 1: 2: 2: 2) 17.6 Example 4 Truffle Mycelium: Mountain Mulberry: Bamboo: Strawberry: Jujube
(2: 2: 1: 1: 1) 20.3 Vitamin C (positive control) 2.2

The antioxidant power of the extracts was calculated by the vitamin C standard curve and the IC ₅₀ was calculated based on the dose response curve. The degree of extinction of the sample was expressed as ㎍ vitamin C equivalents per 100 mL (㎍ vitamin C equivalents per 100 mL) considering the correlation with the vitamin C standard curve.

As shown in Table 2, DPPH free radical scavenging activity was insignificant in the mycelium extracts of mushroom mycelia and mung bean extracts, but the DPPH free radical scavenging activity was shown in each of the extracts of Rice bran extract, Strawberry extract and Jujube extract. Compared with the compound extracts of Examples 2 to 4, the extract of the ethanol complex of the truffle mushroom mycelium, the mulberry tree, the pomegranate juice, the jujubate, and the jujube of Example 1 was significantly higher in DPPH free radical scavenging activity. The IC ₅₀ value of the combined extract was 16.1 μg / ml, which was significantly lower than that of the other extracts.

2 is a graph showing DPPH free radical scavenging activity (%) according to the concentration of the complex extract prepared in Example 1 of the present invention. As shown in FIG. 1, DPPH free radical scavenging ability was not higher than that of vitamin C, which was a positive control, at 100 μg / ml and 20 μg / ml of the combined extract, but DPPH free radical scavenging ability was remarkably excellent. Dependent increase in the concentration of the complex extract. Accordingly, it was confirmed that the combined extracts of the mycelia of tinea, mulberry, buckwheat, jujube, and jujube of the present invention have excellent antioxidative activities through inhibition of free radicals promoting the skin aging process.

Thus, the combined extracts of mycelium of truffle mushroom, mulberry tree, pomegranate jujube, jujube, and jujube of the present invention inhibit the free radicals promoting skin aging process and have an excellent antioxidative activity compared to the extracts of truffle mushroom mycelium and mountain mulberry As a result, the antioxidant activity can lower the aging process and the degree of skin damage.

In the case of the single extract, the extracts of tinea mushroom mycelium and mung bean extract showed strong tyrosinase inhibitory activity. However, each of the extracts of Lycopene, Extract, and Jujube showed little inhibitory activity against tyrosinase It did not appear strongly. Conversely, DPPH free radical scavenging activity was negligible in the mycelium extracts of mushroom mycelia and mulberry leaves, respectively. However, DPPH radical scavenging activity was shown to be superior to DPPH extract, jujube extract and jujube extract.

That is, in the case of each extract alone, the extract of tinea mushroom mycelium and mung bean alone showed an excellent inhibitory activity against tyrosinase, but the DPPH free radical scavenging ability was low. In case of extracts of soleus, The tyrosinase inhibitory activity was low but DPPH free radical scavenging ability was excellent. That is, in the case of each single extract, there was no extract having both tyrosinase inhibitory activity and antioxidant activity. Accordingly, in order to provide a composition having both an antioxidative activity and a whitening effect, the present invention provides a combined extract of tinea mushroom mycelium, mulberry tree, syrup, jujube, and jujube.

In conclusion, the combined extracts of the mycelia of tinea, mulberry, sycamore, jujube, and jujube of the present invention inhibit tyrosinase activity, which is a key component of the melanin biosynthesis process, to a remarkably high level, Effectively inhibits the whitening effect and, at the same time, has an excellent antioxidative effect and can be used as a composition for whitening or antioxidation.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, Of the right.

Claims (1)

Mycelium of truffle ( Tuber magnatum mycelium, Morus bombycis), Agastache rugosa (Agastache rugosa), jangttalgi (Rubus hirsutus ) and a jujube ( Zizyphus jujube ) or a fraction thereof as an active ingredient.

Images