KR20170036640A - Medical composition for preventing or treating of alopecia, health functional food and cosmetic composition - Google Patents

Abstract

The present invention relates to a medicinal composition, a health functional food and a cosmetic composition for prevention or treatment of hair loss, which comprises a perennial herb extract, a self-leaf extract and a green tea extract, A health functional food and a cosmetic composition for prevention or treatment of hair loss which can exhibit an excellent hair loss preventive, ameliorating or therapeutic effect by their synergistic effect by containing 25 to 75 parts by weight of a green tea extract .

Description

TECHNICAL FIELD [0001] The present invention relates to a pharmaceutical composition, a health functional food and a cosmetic composition for preventing or treating hair loss,

The present invention relates to a pharmaceutical composition and a health functional food for preventing or treating hair loss.

Hair is one of the major parts of the body derived from the outer cell layer of the skin and is considered to be a sub-structure of the envelope, along with sebaceous glands, sweat glands and nails. Hair is also known as an envelope derivative since it originates from the envelope during embryological growth. Hair is important for the overall appearance of the human body, and despite the development of several medical therapies, many people suffer from hair loss or hair loss.

The cause of hair loss is discussed in terms of male hormone hyperactivity, excessive sebaceous secretion, poor blood circulation, peroxidation, hypofunction of the bacterium, lowering of collagen production in the papilla, genetic factors, aging and stress come. However, the cause of hair loss has not been clarified to date. Recently, the number of hair loss is increasing due to changes in diet and stress due to social environment, age is lowered, and female hair loss population is increasing.

Conventionally, in order to treat or prevent hair loss, there are blood circulation promotion, hair follicle enhancement, scalp moisturizing and the like. Commercially available medicines include female hormone-based preparations for suppressing male hormone and hypertensive drugs for controlling blood pressure, , Which is based on the side effect of hair growth, is sold. However, there is a side effect problem such as a decrease in strength, and when the use is stopped, the effect is not sustained and it returns to the state before use. It is true.

Therefore, it is urgent to develop a therapeutic method and a pharmaceutical composition for prevention of hair loss, hair growth and hair growth using a component derived from natural materials and having no side effects.

Korean Patent No. 10-0800359 discloses a composition exhibiting hair loss prevention and hair growth promoting effect.

Korean Patent No. 10-0800359

It is an object of the present invention to provide a pharmaceutical composition for preventing or treating hair loss derived from natural materials.

It is an object of the present invention to provide a health functional food for preventing or ameliorating hair loss derived from natural materials.

1. Includes Houttuynia cordata extract, Liliaceae extract and Green tea extract,

25 to 75 parts by weight of a self-leaf extract, and 25 to 75 parts by weight of an extract of green tea, based on 100 parts by weight of the perennial herb extract.

2. The pharmaceutical composition for prevention or treatment of hair loss according to 1 above, wherein the extract of Rhododendron japonica extract, Lilium extract and Green tea extract are independently extracted and mixed.

3. The pharmaceutical composition for prevention or treatment of hair loss according to 1 above, wherein the perennial herb extract, green leaf extract and green tea extract are not fermented.

4. The pharmaceutical composition for preventing or treating hair loss according to 1 above, which further comprises at least one extract selected from the group consisting of black soybean extract, glutinous rice extract, seaweed extract, cinnabar extract, hanicincho extract, Puerariae radix extract and licorice root extract.

5. Includes Houttuynia cordata extract, Liliaceae extract and Green tea extract,

25 to 75 parts by weight of a self-leaf extract, and 25 to 75 parts by weight of an extract of green tea, based on 100 parts by weight of the perennial herb extract, to prevent or ameliorate hair loss.

6. The health functional food for preventing or ameliorating hair loss according to 5 above, wherein the perennial herb extract, the self-leaf extract and the green tea extract are independently extracted and mixed.

7. The health functional food for preventing or ameliorating hair loss as set forth in the above 5, wherein the perilla plant extract, self-leaf extract and green tea extract are not fermented.

8. The health functional food for preventing or improving hair loss according to item 5, further comprising at least one extract selected from the group consisting of black soybean extract, glutinous rice extract, seaweed extract, cinnabar extract, .

9. It contains extracts of Rhododendron japonica, Lilium extract and Green tea extract,

25 to 75 parts by weight of a self-leaf extract, and 25 to 75 parts by weight of a green tea extract, based on 100 parts by weight of the perennial herb extract, to prevent or ameliorate hair loss.

10. The cosmetic composition for prevention or improvement of hair loss according to any one of the above 9 to 10, wherein the extract of Rhododendron japonica extract, Lilium extract and Green tea extract are independently extracted and mixed.

11. The cosmetic composition for preventing or ameliorating hair loss according to any one of the above 9, wherein the Hwasungcho extract, the Lilium extract and the green tea extract are not fermented.

12. The cosmetic composition for prevention or improvement of hair loss according to claim 9, further comprising one or more extracts selected from the group consisting of black bean extract, glutinous rice extract, kelp extract, cinnabar extract, hanikuncho extract, Puerariae Radix extract and licorice extract.

The pharmaceutical compositions, health functional foods and cosmetic compositions for prevention or treatment of hair loss according to the present invention exhibit excellent hair loss prevention, improvement or therapeutic effect.

Fig. 1 shows the result of visual observation of the state of mouse hair.
Figure 2 shows the H & E staining results of mouse skin tissue.
Figure 3 shows the expression of IGF-1 and TGF-beta in mouse skin.
Fig. 4 shows the expression levels of IGF-1 and TGF-p1 in rat skin tissue.

The present invention relates to a medicinal composition, a health functional food and a cosmetic composition for prevention or treatment of hair loss, which comprises a perennial herb extract, a self-leaf extract and a green tea extract, A health functional food and a cosmetic composition for prevention or treatment of hair loss which can exhibit an excellent hair loss preventive, ameliorating or therapeutic effect by their synergistic effect by containing 25 to 75 parts by weight of a green tea extract .

Hereinafter, the present invention will be described in detail.

The medicinal composition for prevention or treatment of hair loss according to the present invention comprises a perennial herb extract, a self-leaf extract and a green tea extract, wherein 25 to 75 parts by weight of a self-leaf extract and 25 to 75 parts by weight of a green tea extract are added to 100 parts by weight of the above- .

Hwasungcho has antimicrobial and anti-inflammatory effects and is excellent in antimicrobial effect against hairless bacteria such as Malasejia and Staphylococcus aureus.

The lobulated lobes are rich in minerals, vitamins B1, B2, B6, vitamin C, vitamin E, vitamin K and niacin. They contain calcium, potassium, iron, magnesium and zinc as well as alpha-linolenes that break down cholesterol in the body. And contains almost all of the coenzyme components required for the human body. In addition, ferulaldehyde and beta-carotene exhibit strong antimicrobial activity and antioxidant activity, and exhibit excellent synergistic effects on hair loss when used together with the ecstasy.

Green tea is an evergreen tree or shrub belonging to Theaceae. The polyphenols found in green tea are known as flavonol and catechin, and have various effects such as antioxidant, anti-cancer, anti-inflammation, and antibacterial effects.

According to the present invention, when the mixing ratio of the Hwasung extract, the green leaf extract and the green tea extract is within the above range, the effect of preventing or treating hair loss may be excellent.

From the viewpoint of maximizing the synergistic effect of the green tea leaf, green leaf, and green tea, preferably 55 to 75 parts by weight of the self-leaf extract and 55 to 75 parts by weight of the green tea extract are contained in 100 parts by weight of the extract, May be contained in an amount of 60 to 75 parts by weight of the self-leaf extract and 60 to 75 parts by weight of the green tea extract, based on 100 parts by weight of the perennial herb extract. For example, the Hwasung extract, the green leaf extract, and the green tea extract may be contained in an amount of 70 parts by weight of the self-leaf extract and 70 parts by weight of the green tea extract, respectively, based on 100 parts by weight of the Hwasung extract.

The extracts of the above-mentioned medicinal materials may be those prepared by conventional methods for producing plant extracts. For example, the extracts of flowers, leaves, branches, roots, fruits or shells of the respective plants, Or extracting with an extraction solvent, and fractionating the crude extract with a fraction solvent.

The extraction solvent may be at least one selected from the group consisting of water and an organic solvent. The organic solvent may be a polar solvent such as an alcohol having 1 to 5 carbon atoms, a diluted alcohol such as ethyl acetate or acetone, a nonpolar solvent such as ether, chloroform, benzene, hexane or dichloromethane, or a mixture thereof. The alcohol having 1 to 5 carbon atoms may be methanol, ethanol, propanol, butanol, or isopropanol, but is not limited thereto. In addition, the alcohol dilution water may be an alcohol diluted with water at 50 to 99.9% (v / v).

The extraction solvent used to obtain the extract of the present invention may preferably be at least one selected from the group consisting of water, an alcohol having 1 to 5 carbon atoms, an alcohol-diluted water, and a mixture thereof, more preferably water, To 4% alcohol, and mixtures thereof, and most preferably from 30% to 99% ethanol aqueous solution or alcohol. The extraction process may be performed, for example, at 4 ° C to 120 ° C, or 50 ° C to 90 ° C, or 70 ° C to 85 ° C, but is not limited thereto. The extraction time is not particularly limited, but may be 10 minutes to 12 hours, preferably 1 hour to 6 hours.

Specific extracting methods include a cold extraction method, a warm-up extraction method, a heat extraction method, and an ultrasonic extraction method. Conventional extraction equipment, an ultrasonic pulverization extractor, or a fractionator can be used.

Further, the extract extracted with the solvent may be further fractionated with any one solvent selected from the group consisting of hexane, methylene chloride, acetone, ethyl acetate, ethyl ether, chloroform, water, and mixtures thereof. Two or more kinds of solvents may be used for the fractionation, and the solvent extracts may be sequentially used or mixed according to the polarity of the solvent. In addition, the fractionation process may be performed, for example, at 4 ° C to 120 ° C, or 50 ° C to 90 ° C, or 70 ° C to 85 ° C, but is not limited thereto.

The thus-prepared extract or the fraction obtained by performing the fractionation process may be filtered, concentrated or dried to remove the solvent, and may be subjected to both filtration, concentration and drying. Specifically, the filtration can be performed using a filter paper or a vacuum filter, and the concentration can be reduced by using a vacuum concentrator, for example, a rotary evaporator. The drying can be performed by, for example, freeze drying.

According to one embodiment of the present invention, the extracts of Rhododendron japonica, Lilium extract and Green tea extract may be independently extracted and mixed.

When the medicaments are independently extracted and then mixed, it is possible to exhibit a hair loss preventive and therapeutic effect, which is superior to extracting the oriental herb, domestic leaf and green tea at the same time.

According to one embodiment of the present invention, the Hwasungcho extract, the Lilium extract and the green tea extract may not be fermented. Therefore, the problem of process control such as corruption may not occur, and the process may be simple and the process may be performed in a short time.

 According to one embodiment of the present invention, the pharmaceutical composition for preventing or treating hair loss may further comprise Angelica gigantosa extract, Radix ginseng extract and Gardenia extract, and the content ratio thereof is not limited. However, 25 to 75 parts by weight of ginseng extract and 15 to 50 parts by weight of gardenia extract.

Angelica is a root from the root of new persimmon and improves the blood circulation of the scalp.

Dansam is a medicinal product made from the roots of Salvia miltiorrhiza Bunge (Korea, China). It helps circulation, eliminates eosinophilia, and relieves pain in the extremities. Men's physiological impoverishment, menstrual cramps, postpartum abdominal pain, abdominal fullness of the abdomen, bruises, etc. are treated. It treats mental confusion, bullshit, fury, insomnia, skin rash, palpitation, etc. due to high fever. Pharmacological actions have been reported for coronary artery enlargement, cholesterol depression, hypotension, liver function activation, sedative anti-inflammatory, anti-cancer and anti-bacterial effects.

Gardenia is a fruit of gardenia and copepods and is a fruit of gardenin, pectin, tannin, crocin, gardenoside, geniposide, (scandoside) are known, and ginger, blood pressure lowering effect has been known.

According to another embodiment of the present invention, the pharmaceutical composition for prevention or treatment of hair loss includes at least one extract selected from the group consisting of black bean extract, glutinous rice extract, seaweed extract, cinnabar extract, .

Each of the medicinal plant extracts may have been extracted according to the method described above.

Each of the above-mentioned medicinal plant extracts can further increase the effect of preventing or improving hair loss.

The pharmaceutical composition of the present invention may be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral preparations, suppositories and sterilized injection solutions according to a conventional method.

Examples of carriers, excipients and diluents that may be contained in the composition containing the extract of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, , Alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil have. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used.

Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, sucrose or lactose , Gelatin, and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, simple diluents commonly used, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included .

Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The dosage of the pharmaceutical composition of the present invention may vary depending on the age, sex and body weight of the patient, but may be 0.1 to 100 mg / kg, preferably 1 to 10 mg / kg, once to several times per day. The dosage may also be increased or decreased depending on the route of administration, degree of disease, sex, weight, age, and the like. Accordingly, the dosage is not limited in any way to the scope of the present invention.

The medicinal composition of the present invention can be used for female hair loss and circular hair loss such as O-shaped hair loss, M-shaped hair loss, OM compound hair loss, lateral hair loss, genetic hair loss, male hair loss, Can be used for a variety of hair loss symptoms.

The health functional food for prevention or amelioration of hair loss according to the present invention comprises a perennial herb extract, a self-leaf extract and a green tea extract, wherein 25 to 75 parts by weight of a self-leaf extract and 25 to 75 parts by weight of a green tea extract, .

Each of the extracts may have been extracted according to the aforementioned method.

According to the present invention, hair loss prevention or amelioration effect may be excellent when the blending ratio of Hwasung extract, green leaf extract and green tea extract is within the above range.

From the viewpoint of maximizing the synergistic effect of the green tea leaf, green leaf, and green tea, preferably 55 to 75 parts by weight of the self-leaf extract and 55 to 75 parts by weight of the green tea extract are contained in 100 parts by weight of the extract, May be contained in an amount of 60 to 75 parts by weight of the self-leaf extract and 60 to 75 parts by weight of the green tea extract, based on 100 parts by weight of the perennial herb extract. For example, the Hwasung extract, the green leaf extract, and the green tea extract may be contained in an amount of 70 parts by weight of the self-leaf extract and 70 parts by weight of the green tea extract, respectively, based on 100 parts by weight of the Hwasung extract.

According to one embodiment of the present invention, the extracts of Rhododendron japonica, Lilium extract and Green tea extract may be independently extracted and mixed.

When the medicaments are independently extracted and then mixed, it is possible to exhibit a better hair loss preventing and improving effect than extracting simultaneously the green tea leaf, green leaf and green tea at the same time.

According to one embodiment of the present invention, the Hwasungcho extract, the Lilium extract and the green tea extract may not be fermented. Therefore, the problem of process control such as corruption may not occur, and the process may be simple and the process may be performed in a short time.

According to one embodiment of the present invention, the health functional food for preventing or ameliorating hair loss may further comprise Angelica gigantosa extract, Radix ginseng extract and Gardenia extract, and the content ratio thereof is not limited. However, 50 to 100 parts by weight, ginseng extract 25 to 75 parts by weight and ginger extract 15 to 50 parts by weight.

According to another embodiment of the present invention, one or more extracts selected from the group consisting of black soybean extract, glutinous rice extract, seaweed extract, cinnabar extract, licorice extract, Puerariae radix and licorice extract may be further included.

Each of the medicinal plant extracts may have been extracted according to the method described above.

Each of the above-mentioned medicinal plant extracts can further increase the effect of preventing or improving hair loss.

The health functional food of the present invention can be used as a health functional food containing the above extract (including an alcoholic beverage), fruit and its processed food (e.g., canned fruit, jar, jam, maralade, etc.), fish, (Eg udon, buckwheat noodles, ramen noodles, spaghetti, macaroni, etc.), juice, various drinks, cookies, candy, dairy products such as butter, cheese, Margarine, vegetable protein, retort food, frozen food, various seasonings (eg, miso, soy sauce, sauces, etc.).

In addition, the health functional food of the present invention may be formulated into tablets, pills, powders, granules, powders, capsules, liquid preparations and the like. These may be formulated further comprising one or more of carriers, diluents, excipients and additives.

Examples of the additive which can be further included in the present invention include natural carbohydrates, flavors, nutrients, vitamins, minerals (electrolytes), flavors (synthetic flavors, natural flavors and the like), colorants, fillers At least one component selected from the group consisting of acids and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, preservatives, antioxidants, glycerin, alcohols, carbonating agents and fats can be used.

Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavors (tautatin, stevia extract (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharine, aspartame, etc.) can be advantageously used as the flavor.

In addition to the above, the health functional food of the present invention may contain flavorings such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, colorants and heavies (cheese, chocolate etc.), pectic acid and its salts, And salts thereof, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. In addition, the health functional food of the present invention may contain flesh for the production of natural fruit juice and vegetable beverages. These components may be used independently or in combination.

Specific examples of the carrier, excipient, diluent and additives include, but are not limited to, lactose, dextrose, sucrose, sorbitol, mannitol, erythritol, starch, acacia rubber, calcium phosphate, alginate, gelatin, calcium phosphate, calcium Silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, polyvinylpyrrolidone, methylcellulose, water, sugar syrup, methylcellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate And a mineral oil are preferably used.

The content of the health functional food of the present invention as the active ingredient in the above-described formulation can be appropriately adjusted depending on the mode and purpose of use, the condition of the user, the type of symptoms and the severity, and is 0.001 to 99.9% by weight, May be 0.01 to 50% by weight, but is not limited thereto.

The cosmetic composition for preventing or ameliorating hair loss according to the present invention may further comprise 25 to 75 parts by weight of a self-leaf extract and 25 to 75 parts by weight of green tea extract per 100 parts by weight of the extract of green tea extract, Parts by weight.

Each of the extracts may have been extracted according to the aforementioned method.

According to the present invention, hair loss prevention or amelioration effect may be excellent when the blending ratio of Hwasung extract, green leaf extract and green tea extract is within the above range.

From the viewpoint of maximizing the synergistic effect of the green tea leaf, green leaf, and green tea, preferably 55 to 75 parts by weight of the self-leaf extract and 55 to 75 parts by weight of the green tea extract are contained in 100 parts by weight of the extract, May be contained in an amount of 60 to 75 parts by weight of the self-leaf extract and 60 to 75 parts by weight of the green tea extract, based on 100 parts by weight of the perennial herb extract. For example, the Hwasung extract, the green leaf extract, and the green tea extract may be contained in an amount of 70 parts by weight of the self-leaf extract and 70 parts by weight of the green tea extract, respectively, based on 100 parts by weight of the Hwasung extract.

According to one embodiment of the present invention, the extracts of Rhododendron japonica, Lilium extract and Green tea extract may be independently extracted and mixed.

When the medicaments are independently extracted and then mixed, it is possible to exhibit an effect of preventing and alleviating hair loss, which is superior to the simultaneous extraction of the oriental herb, domestic leaf and green tea.

According to one embodiment of the present invention, the Hwasungcho extract, the Lilium extract and the green tea extract may not be fermented. Therefore, the problem of process control such as corruption may not occur, and the process may be simple and the process may be performed in a short time.

According to one embodiment of the present invention, the cosmetic composition for preventing or ameliorating hair loss may further comprise Angelica gigantosa extract, Radix ginseng extract and Gardenia extract, and the content ratio thereof is not limited. However, 25 to 75 parts by weight of ginseng extract and 15 to 50 parts by weight of gardenia extract.

According to another embodiment of the present invention, the cosmetic composition for prevention or improvement of hair loss includes at least one extract selected from the group consisting of black soybean extract, glutinous rice extract, seaweed extract, cinnabar extract, .

Each of the medicinal plant extracts may have been extracted according to the method described above.

Each of the above-mentioned medicinal plant extracts can further increase the effect of preventing or improving hair loss.

The cosmetic composition of the present invention may further comprise a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides, sphingolipids and seaweed extracts.

Examples of the water-soluble vitamin include vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic amide, folic acid, vitamin C and vitamin H And salts thereof (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbic acid-2-phosphate, magnesium ascorbic acid-2-phosphate etc.) can also be used as water-soluble vitamins . The water-soluble vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzymatic method, or a chemical synthesis method.

Usable vitamins include vitamins such as vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol) , And derivatives thereof (such as palmitic acid ascorbin, stearic acid ascorbic acid, dipalmitic acid ascorbic acid, dl-alpha tocopheryl acetate, dl-alpha tocopherol nicotinic acid vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, Ethyl ether, etc.) and the like are also included in the usable vitamins used in the present invention. Usability Vitamins can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzyme or a chemical synthesis method.

The polymeric peptide may be any compound as long as it can be compounded in cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin and the like can be mentioned. The polymeric peptide can be obtained by a conventional method such as purification from a culture broth of a microorganism, an enzymatic method, or a chemical synthesis method, or it can be purified from natural products such as ducks such as pigs and cows and silk fiber of silkworms.

The polymeric polysaccharide may be any compound as long as it can be incorporated in cosmetics, and examples thereof include hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.). For example, chondroitin sulfate or a salt thereof can be usually purified from mammals or fish.

Sphingo lipids may be any as long as they can be incorporated into cosmetics, and preferable examples thereof include ceramides, phytosphingosine and sphingoglycolipids. Sphingoid lipids can be purified from ordinary mammals, fish, shellfish, yeast or plants by conventional methods or can be obtained by chemical synthesis.

The seaweed extract may be any of those which can be compounded in cosmetics. Preferably, the seaweed extract is selected from the group consisting of algae extract, red pepper extract, green algae extract and the like. Also, the algae extract may be colored guanine, arginic acid, Potassium alginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained from seaweed by a conventional method.

The cosmetic composition of the present invention may further contain other essential ingredients, if necessary, in combination with the cosmetic composition.

Examples of the components that can be blended include a preservative component, a moisturizer, an emollient, a surfactant, an organic and inorganic pigment, an organic powder, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a plant extract, a pH adjuster, , A cold agent, an antiperspirant agent, and purified water.

Examples of the fat-soluble ingredients include ester-based fats, hydrocarbon-based fats, silicon-based fats, fluorine-based fats, animal fats and plant fats and the like.

Examples of ester-based fats include glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, But are not limited to, ethylbutyl, butylbutyl, ethylbutylbutyl, butylbutyl, isobutylbutyl, isobutylbutyl, isobutylisobutyl, isobutylisobutyl, isobutylisobutyl, Trimethylol propane, triisostearic acid trimethylol propane, tetra-2-ethylhexanoic acid pentaerythritol tri (2-ethylhexanoic acid) Terephthalic acid, stearic acid, tallow, caprylic acid, decyl lauric acid, hexyl laurate, myristic acid myristate, myristyl myristate, myristyl acid stearyl, stearyl stearate, decyl oleate, cetyl ricinoleate, Octyldodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, isopropyl stearate, isostearyl stearate, isocetyl stearate, isodecyl oleate, isodecyl oleate, octyldodecyl oleate, Ethylhexanoate, stearylhexyl isostearate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, di (capryl, capric acid) propylene glycol, dicapryl Diethylene glycol monobutyl ether, diethylene glycol propylene glycol, dicapric acid neopentyl glycol, dioctanoic acid neopentyl glycol, tricarboxylic acid glyceryl, triunsaturated glyceryl, triisopalmitic acid glyceryl, triisostearic acid glyceryl, Octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, Octyldecyl lactate, octyldecyl stearate, polyglycerin oleic acid ester, polyglycerin isostearic acid ester, triisocetyl citrate, triisolealkyl citrate, triisooctyl citrate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate , Acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, Cholesteryl isostearate, chitostearic acid, cholestearyl isostearate, cholestearyl hydroxystearate, cholesteryl oleate, dihydrocholestearyl oleate, pitostearyl isostearate, pitostearyl oleate, 12 -Stearoylhydroxystearic acid isostearyl, 12-stearoylhydroxystearic stearyl, 12-stearoyl And esters such as hydroxystearic acid and sostearyl.

Hydrocarbon-based fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, floating isoparaffin, polybutene, microcrystalline wax, and hydrogenated hydrocarbon such as vaseline.

Examples of silicone based oils include polymethyl silicone, methylphenyl silicone, methyl cyclopolysiloxane, octamethyl polysiloxane, decamethyl polysiloxane, dodecamethyl cyclosiloxane, dimethylsiloxane-methyl cetyloxysiloxane copolymer, dimethylsiloxane-methyl stearoxysiloxane copolymer, Alkyl-modified silicone oil, and amino-modified silicone oil.

Examples of the fluorine-based oil include perfluoropolyether and the like.

Examples of the animal or vegetable oil include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, new flower oil, soybean oil, corn oil, rape oil, apricot kernel oil, palm kernel oil, palm oil, castor oil, sunflower oil, The present invention relates to a process for producing a starch-containing starch, which is selected from the group consisting of seed oil, cottonseed oil, palm oil, palm oil, cucumber nut oil, wheat germ oil, rice germ oil, Animal or vegetable fats such as oil, can-pick wax, carnauba wax, liquid lanolin, and hardened castor oil.

Examples of the moisturizing agent include water-soluble low-molecular moisturizing agents, oil-soluble molecular moisturizing agents, water-soluble polymers, and oil-soluble polymers.

Examples of the water-soluble low molecular weight moisturizing agent include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B Propylene glycol (polymerization degree n = 2 or more), polyglycerin B (polymerization degree n = 2 or more), lactic acid, lactic acid salt and the like.

Examples of the lipid-soluble low-molecular moisturizing agent include cholesterol and cholesterol ester.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartic acid, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, .

Examples of the oil-soluble polymer include a polyvinylpyrrolidone / eicosene copolymer, a polyvinylpyrrolidone / hexadecene copolymer, a nitrocellulose, a dextrin fatty acid ester, and a polymeric silicone.

The emollients include long chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl ester, and the like.

Examples of the surfactant include a nonionic surfactant, an anionic surfactant, a cationic surfactant, and a positive surfactant.

Examples of nonionic surfactants include self emulsifying monostearic acid glycerin, propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, sorbitan fatty acid esters, POE (polyoxyethylene) sorbitan fatty acid esters, POE sorbit fatty acid esters, POE-POP fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POE.POP (polyoxyethylene.polyoxypropylene) copolymer, POE.POP alkyl ether, polyether- Acid alkanolamides, alkylamine oxides, hydrogenated soybean phospholipids, and the like.

Examples of the anionic surfactant include fatty acid soap, alpha-acylsulfonate, alkylsulfonate, alkylarylsulfonate, alkylnaphthalenesulfonate, alkylsulfate, POE alkylether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl ginseng salt, alkyl Amide phosphate, alkyloyl taurine salt, N-acyl amino acid salt, POE alkyl ether carboxylate, alkylsulfosuccinate, sodium alkylsulfoacetate, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate ester and the like .

Examples of the cationic surfactant include alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, Benzalkonium chloride, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, quaternary ammonium salts of lanolin derivatives, and the like.

Examples of amphoteric surfactants include carboxybetaine, amidebetaine, sulfobetaine, hydroxysulfobetaine, amidosulfobetaine, phosphobetaine, aminocarboxylate, imidazoline derivative, amideamine, etc. , And the like.

Examples of the organic and inorganic pigments include inorganic pigments such as silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, Bengala, clay, bentonite, titanium mica, titanium oxide, bismuth oxychloride, zirconium oxide, magnesium oxide, Inorganic pigments such as aluminum, calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, chromium oxide, chromium oxide, chromium oxide, But are not limited to, polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, Silk powder, cellulose, CI Pigment Yellow, CI Pigment Orange, and composite pigments of inorganic pigments and organic pigments thereof.

Examples of the organic powder include metal soaps such as calcium stearate; Metal salts of alkyl phosphates such as sodium zinc cetylate, zinc laurylate and calcium lauryl laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc and N-lauroylglycine calcium; Amidosulfonic acid multivalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; Such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylidene, N-alpha-paratyylnitine, N-alpha-lauroyl arginine, Acyl basic amino acids; N-acylpolypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid, alpha-aminoaurauric acid, and the like; Polyethylene, polypropylene, nylon, polymethylmethacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride, and the like.

Examples of the ultraviolet absorbing agent include pamoaminobenzoic acid, ethyl p-aminobenzoate, amyl p-aminobenzoate, octyl p-aminobenzoate, ethylene glycol salicylate, phenyl salicinate, benzyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, Benzyl, 2-ethoxyethyl methoxycinnamate, octyl methoxycinnamate, mono-2-ethylhexane glyceryl dipyrromethoxycinnamate, isopropyl methoxycinnamate, diisopropyl-diisopropyl cinnamate ester mixture, Dihydroxymethoxybenzophenone sulfonic acid, dihydroxybenzophenone sulfonic acid, dihydroxybenzophenone sulfonic acid and its salt, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzo Phenanone, tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianylino-p- (carbo-2'-ethylhexyl- 1,3,5-triazine, 2- (2-hydroxy -5-methylphenyl) benzotriazole, and the like.

Examples of the bactericide include hinokitiol, trichloroacetic acid, trichlorohydroxydiphenyl ether, crohexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, No. 301, mononitro and eicoll sodium, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, gallic acid propyl, and eicosorbic acid.

Examples of the pH adjusting agent include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

Any of the above components may be blended to the extent that the object and effect of the present invention are not impaired, but it is preferably 0.01 to 5% by weight based on the total weight, Preferably 0.01 to 3% by weight.

The cosmetic composition of the present invention may take the form of a solution, an emulsion, a viscous mixture or the like.

The ingredients contained in the cosmetic composition of the present invention may contain, as an active ingredient, the ingredients commonly used in cosmetic compositions in addition to the above-mentioned pharmacologic extracts. Examples of such ingredients include conventional ones such as stabilizers, solubilizers, vitamins, Adjuvants and carriers.

The cosmetic composition of the present invention can be prepared into any formulation conventionally produced in the art, and examples thereof include emulsions, creams, lotions, packs, foundations, lotions, essences, and hair cosmetics.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

The cosmetic composition of the present invention can be used for cosmetics such as hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nourishing cream, Hair dye, hair gel, hair glaze, hair dresser, hair lacquer, hair moisturizer, hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, A hair mousse, a hair spray, and the like, but the present invention is not limited thereto.

The medicinal composition of the present invention can be used for female hair loss and circular hair loss such as O-shaped hair loss, M-shaped hair loss, OM compound hair loss, lateral hair loss, genetic hair loss, male hair loss, Can be used for a variety of hair loss symptoms.

Hereinafter, the present invention will be described in detail by way of examples with reference to the following examples.

Example

In vitro experiments

1. Experimental material

Extracts were prepared by filtration and hot-air drying or spray-drying to extract 1 g of Sophora sinensis extracts in 15 ml of ethanol (30%), followed by extraction at 86 ° C for 3 hours.

To 15 ml of 30% ethanol, 1 g of the baby leaf was added and extracted at 86 ° C for 3 hours. The extract was filtered and concentrated by hot air drying or spray drying.

The green tea extract was prepared by adding 1 g of green tea to 15 ml of 30% ethanol and extracting it at 86 ° C for 3 hours, followed by filtration, hot air drying or spray drying.

The extracts were mixed at the content ratios shown in Table 1 (mixing after extraction).

division Horsetail extract (parts by weight) Leaf lobule extract
(Parts by weight) Green tea extract
(Parts by weight) Example 1 100 30 30 Example 2 100 50 50 Example 3 100 70 70 Example 4 100 60 40 Comparative Example 1 100 20 60 Comparative Example 2 100 60 20 Comparative Example 3 100 10 20 Comparative Example 4 100 80 80

In addition, Examples 5 to 10 of Table 2 below were made to compare effects of fermentation and extraction methods with those of the examples.

In Example 5, 15 g of 30% ethanol was extracted with 3 g of 3 g of each of 1 g of Seaweed, 1 g of Green Leaf and 1 g of green tea at 86 ° C for 3 hours. Then, under the temperature condition of 15 to 25 ° C and the relative humidity of 50 to 60% Fermentation for one month, filtration concentration and hot air drying or spray drying extract.

In the case of Example 6, 2 g of hollobes, 1 g of baby leaf and 1 g of green tea were mixed with 60 ml of ethanol 30%, and then the mixture was extracted at 86 ° C for 3 hours, followed by filtration, hot air drying or spray drying to prepare an extract.

In the case of Example 7, 60 g of ethanol 30% was mixed with 2 g of hemp seed, 1 g of hemp leaf and 1 g of green tea, and the mixture was extracted at 86 캜 for 3 hours and then subjected to a temperature condition of 15 to 25 캜 and a relative humidity of 50 to 60% For 3 months, filtration concentrated and hot air drying Or spray-dried extract.

In Example 8, 15 g of 30% ethanol was extracted with 3 g of 86 g / L of 1 g of Seaweed, 1 g of Green Leaf, and 1 g of Green Tea, and the resulting mixture was extracted under the conditions of temperature of 15 to 25 ° C and relative humidity of 50 to 60% Fermentation for one month, filtration concentration and hot air drying or spray drying extract.

In the case of Example 9, the extract was prepared by mixing 2 g of hemolymph, 1 g of hemp leaf and 1 g of green tea in 60 ml of ethanol 30%, and then extracting it at 86 ° C for 3 hours, followed by filtration, hot air drying or spray drying.

In Example 10, 60 g of 30% ethanol was mixed with 2 g of hemp seed, 1 g of hemp leaf and 1 g of green tea, extracted at 86 DEG C for 3 hours, and then subjected to a temperature condition of 15 to 25 DEG C and a relative humidity of 50 to 60% For 3 months, and then mixed with filtration concentrated, hot air dried or spray dried extract.

division Stamina: Lobulus: Green tea
(Parts by weight) Whether it is fermented Extraction method Example 5 100: 50: 50 0 After extraction Example 6 100: 50: 50 X Mixed extraction Example 7 100: 50: 50 0 Mixed extraction Example 8 100: 60: 40 0 After extraction Example 9 100: 60: 40 X Mixed extraction Example 10 100: 60: 40 0 Mixed extraction

2. BrdU assay

(1) Principle of experiment

Halogenated nucleotides such as the pyrimidine analogue bromodeoxyuridine (BrdU) are useful for staining nascent (early) DNA in living cells or tissues. In the "S" phase, when DNA in the nucleus is duplicated in the cell cycle, BrdU is used instead of thymidine of the DNA being duplicated, and the amount of DNA synthesized through BrdU antibody staining is checked .

(2) Experimental method

1) Suspension with 1 ml of culture medium in a cell pellet tube.

2) 10 μl was added to a haemacytometer (hemocytometer), and the number of cells was observed under a microscope.

3) For the experiment, 1 ㅧ 10⁴ cells were seeded in 96 wells. At this time, 100 μl of media per well was added. After seeding, wells were stored in a 37 ° C CO ₂ incubator.

4) After being stored for 24 hours in a 37 ° C CO ₂ incubator, the cells were treated according to the compositions of Examples and Comparative Examples and stored in a 37 ° C CO ₂ incubator.

5) After incubation at 37 ° C in CO ₂ incubator for 24 hours, 10 μl of 10 × BrdU solution was added, incubated for 1 hour, and medium was removed.

6) After adding 100 ㎕ fixation / denaturing solution for 30 minutes at room temperature, solution was removed and 100 ㎕ of 1X Dectection antibody solution was added and left at room temperature for 1 hour.

7) Washing with washing buffer 3 times.

8) 1 × HRP-conjugated secondary antibody solution was treated with 100 μl per well and incubated at room temperature for 30 minutes.

9) and washed 3 times with washing buffer.

10) Add 100 μl of TMB substrate to each well, store at room temperature for 30 minutes, and add 100 μl of STOP solution to each well.

11) The absorbance of each well was measured at a wave length of 450 nm using an ELISA reader.

(3) Experimental results

Table 3 shows absorbance values according to the treatment concentration in the examples, and it means that the higher the absorbance, the better the effect of prevention, improvement or treatment of hair loss.

Average value / error value Treatment concentration 1 [mu] g / ml 10 [mu] g / ml 30 ug / ml Example 1 103.986 / 1.295 112.185 / 1.397 121.468 / 1.513 Example 2 102.327 / 1.728 122.285 / 2.599 133.913 / 2.694 Example 3 105.497 / 1.211 125.722 / 1.467 135.273 / 1.63 Example 4 102.1 / 2.276 110.2 / 2.279 121 / 2.525 Example 5 95.1 / 2.142 94.4 / 2.128 108.4 / 2.413 Example 6 93.82 / 0.576 96.338 / 1.219 102.541 / 1.142 Example 7 92.2 / 0.337 95.5 / 1.295 108.2 / 0.525 Example 8 94.136 / 1.33 94.3 / 1.426 109.735 / 1.609 Example 9 94.4 / 0.36 97.6 / 6.993 104.7 / 0.461 Example 10 93.546 / 0.677 95.862 / 2.704 109.510 / 3.281 Comparative Example 1 88.5 / 0.661 90.11 / 1.51 94.41 / 1.64 Comparative Example 2 84.71 / 1.346 91.7 / 2.63 95.74 / 0.452 Comparative Example 3 80.56 / 0.642 83.99 / 1.99 89.99 / 0.474 Comparative Example 4 90.6 / 0.364 93.7 / 2.14 97.78 / 2.642

3. MTT assay

(1) Principle of experiment

The MTT assay is a colorimetric assay that measures the absorption of specific wavelengths through color change. The mitochondrial reduction of MTT tetrazolium, a water soluble substrate of yellow by dehydrogenase, to purple, water-insoluble MTT formazan (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl-tetrazolium bromide) It is a test using ability. The higher the absorbance, the greater the number of living cells. The smaller the absorbance, the greater the number of dead cells.

(2) Experimental method

1) Using a 1 ml pipette, 1 ml of culture medium was placed in a 15-ml tube with cell pellet and tappin.

2) 10 μl was added to a Haemacytometer (blood counting instrument), and the number of cells was observed under a microscope.

3) For MTT experiment, 1 ㅧ 10⁴ cells were seeded in 96 wells. At this time, 100 μl of media per well was added.

4) Cells were seeded and stored in a 37 ° C CO ₂ incubator.

5) After storage for 24 hours in a CO ₂ incubator, the samples were treated according to the compositions of the examples and comparative examples and stored in a 37 ° C CO ₂ incubator.

6) After storage for 24 hours in a CO ₂ incubator, media was removed from the wells using a media sorter.

7) 100 μl of DPBS was added per well and washed.

8) Dilute 10 μl of MTT solution in 90 μl of culture medium, and add 100 μl of each solution to each well.

9) 30 minutes to 4 hours at 37 ℃ CO ₂ incubator. (Each experiment used the same incubator.)

10) Absorbance was measured at a wavelength of 450 nm using an ELISA reader.

(3) Experimental results

Table 4 shows the absorbance values according to the treatment concentration in the examples, and it means that the higher the absorbance, the better the prevention, improvement or treatment effect of hair loss.

Average value / error value Treatment concentration 1 [mu] g / ml 10 [mu] g / ml 30 ug / ml Example 1 106.082 / 3.611 106.722 / 3.633 111.481 / 3.795 Example 2 105.228 / 0.337 105.529 / 1.295 118.150 / 0.525 Example 3 108.8 / 2.128 115.1 / 2.134 122.2 / 2.453 Example 4 104.13 / 1.279 105.737 / 1.34 111.129 / 1.374 Example 5 98.2 / 2.274 101.9 / 2.36 104.2 / 2.643 Example 6 99.73 / 1.345 102.3 / 1.62 102.1 / 2.213 Example 7 99.4 / 0.36 99.7 / 0.461 105.8 / 3.326 Example 8 97.4 / 2.262 99.8 / 2.27 103.7 / 2.449 Example 9 97.282 / 1.305 100.41 / 1.31 104.335 / 1.36 Example 10 99.728 / 1.344 101.334 / 1.62 104.905 / 5.391 Comparative Example 1 82.2 / 0.471 83.92 / 1.24 91.44 / 4.14 Comparative Example 2 77.71 / 1.244 84.2 / 2.41 93.24 / 2.022 Comparative Example 3 71.56 / 0.762 77.49 / 1.72 89.09 / 2.172 Comparative Example 4 85.6 / 0.194 89.1 / 2.21 95.48 / 1.841

In vivo experiments

1. Animal test purpose

The causes of hair loss include genetic, stress, and aging, but it is known that Hamilton (1942) is the cause of secretion of male hormone after its relationship with male hormone. Dihydrotestosterone (DHT) binds to the androgen receptor of the hair follicle to delay the protein synthesis and shorten the growth period of the hair follicle. The hair follicle grows faster and enters the dormant period, and the proportion of the dormant hair follicle increases. It occurs because it is thin and short. 5-α-reductase, which is present in hair follicle cells, converts testosterone, a male hormone, into DHT, a stronger metabolism. Its hormone activity is about 10 times stronger than normal testosterone and acts on hair follicles to inhibit hair growth.

However, the cause and mechanism of hair loss has not yet been clearly elucidated. A product developed and commercialized at present has a problem that a side effect such as an abnormality occurs in the scalp.

In order to solve the above problems, the present invention was conducted to investigate the in vivo effects of the present invention including natural medicines capable of preventing, treating or improving hair loss.

2. Experimental Method

(1) Animal experiment

Six-week-old male C57BL / 6 mice were purchased and maintained for 24 hours at a temperature of 24 ° C ± 0.5 ° C and a humidity of 55 ± 5%.

When C57BL / 6 mice are 6 to 7 weeks of age, most of the hairs become pauses and the skin color becomes pink. After being adapted to the environment for about 7 days, it was used in the experiment.

Under the anesthesia with intraperitoneal injection, hair was removed from the mouse and the like using care not to damage the skin using a hair clipper, and the animals were randomly divided into polycarbonate breeding areas and divided into groups.

Each natural product complex was administered orally for 4 weeks.

For positive control, oral finasteride was administered at 0.5 mg / day (Hui-Wen Chiu et al. 2013). Oral administration has been performed by a skilled investigator because of the risk of inflammation and damage to the esophagus (or airway) by an invasive method. All animal experiments are conducted according to the guidelines of the Correct Animal Experimental Ethics Committee of the State (2015-0198-01). Details of each group are as follows.

1) Normal rat (n = 3)

2) vehicle-negative control (n = 3)

3) finasteride-positive control (n = 3)

4) Example 3 200 mg / kg (n = 4)

5) Example 3 400 mg / kg (n = 4)

(2) Gross observation

After the start of the experiment, photographs are taken using a digital camera to visually check the condition of the hair. The photographs were taken at the start time, 1 week, 2 weeks and 3 weeks afterwards and graded from 0 to 4 according to the hair condition at the back region, and the results are compared with each group.

0: 0% to 20% of hairs are regenerated

1: When 20% to 40% of hair is regenerated

2: 40% to 60% of hairs were regenerated

3: 60% to 80% of hairs were regenerated

4: 80% to 100% of hairs were regenerated

(3) Histological examination

After 4 weeks from the start of the experiment, all animals are sacrificed and the skin is removed and immediately fixed in 10% formalin. The fixed tissue is dehydrated using alcohol and xylene, embedded in paraffin, and 4 μm thick sections are prepared and stained with Hematoxylin-Eosin to observe the histological changes on the microscope.

(4) Expression of IGF-1, a hair growth factor, and TGF-β1, a hair growth inhibitory and hair-growth factor

To analyze the insulin-like growth factor (IGF-1) and TGF-? 1 contained in the tissues, 50 mg of the skin tissue was stored at -80 ° C. and lysis buffer (50 mM Tris-HCl, 120 mM NaCl, 2 nM EDTA, 1 mM EGTA, and 1% Triton X-100), and the mixture is homogenized using a homogenizer (Tissue tearer, Biospec, Korea), centrifuged and quantified using the Lowly method. Protein was electrophoresed on 15% SDS-PAGE gel, transferred to PVDF paper, blocked with 5% skim milk for 1 hour and primary antibody (IGF-1, TGF-β1, abcam, Cambridge, 5% skim milk at 1: 1000 and then overnight at 4 ° C. After washing with 1X PBST, the secondary antibody (Goat anti-Rabbit IgG) was diluted 1: 1000 in 5% skim milk, reacted for 1 hour, washed, and ECL solution (Amersham, Arlington height, IL, USA) And analyzed with an image analyzer (Image Analiyzer system, Syngene, UK).

3. Results

(1) Gross observation

One day after epilating, the test substance and the test substance were orally administered. Almost no hair growth was observed until 5 days after administration in all experimental groups. The positive and negative test groups showed more hair growth after 15 days than the negative control group. After 25 days, the groups except for the negative control group were overgrown with black hair. Table 5 shows the grades of each group observed with the naked eye.

Hair regrowth score Normal 4.00 ± 0 Negative control 1.33 ± 0.57 Positive control Proscar
0.5 mg / day 3.66 ± 0.57 Example 3 200 mg / kg 3.00 ± 0.81 Example 3 400 mg / kg 3.50 ± 0.57

 (2) Hematoxylin and Eosin (H & E)

To investigate the effect of test substances on hair growth, the effect of hair growth was examined histologically by autopsy after oral administration of the substance.

Positive control and test substance group (Example 3 200 mg / kg and 400 mg / kg) showed a large increase in the number and size of hair follicles, while negative control maintained the hair in a resting state Respectively. In the positive group, the growth of the hair follicle was induced. In the positive group, the growth of the hair follicle was induced by the comparative example. In the group of 200 mg / kg of Example 3, the hair follicle growth was promoted by the growth period. 3 400 mg / kg group seems to shorten the time of hair growth and hair regrowth in the growing period.

The test substances are also different in dose and appear to promote hair follicle growth, promote hair growth and promote hair growth.

(3) IGF-1, TGF-β1 Western blot analysis

As a result of comparing the expression of IGF-1, a hair growth factor, and TGF-β1, a hair growth inhibitory and hair-growth promoting factor, IGF-1 was more expressed in a positive comparison example than in a negative comparison example, Example 3 It was confirmed that the hair growth factor was overexpressed at 200 mg / kg group.

TGF-beta1, an alopecia inducing factor, was overexpressed compared to a positive control. In Example 3, 400 mg / kg group showed lower expression of TGF-beta1 than 200 mg / kg of Example 3.

4. Conclusion

In this experiment, ginseng, japonica, and green tea were orally administered to the complexes of natural products, and gross and histological hair growth effects were confirmed. The C57BL / 6 mouse is a model of hair growth. It has a black hair color and is very similar to human condition histologically or immunologically, and is widely used for study of alopecia. Male C57BL / 6 at 7 weeks of age was used, and the experiment was started after confirming the resting state after hair removal. (Example 3 200 mg / kg and 400 mg / kg) were orally administered after oral administration of distilled water for 4 weeks, positive control-Proscar, Example 3 200 mg / kg or Example 3 400 mg / kg) began to grow more hairy than the negative control group after 15 days. After 25 days, except for the negative control group, the hair grew to cover the entire skin in black. In the H & E test, the negative control group maintained the resting period, the positive control group induced the growth of hair follicles by growing period, the 200 mg / kg group of Example 3 promoted the growth of hair follicles by growing period, In the growing period of the islets, the hair follicle grows fast and the hair regrowth time is shortened.

The expression level of IGF-1 and TGF-β1 was measured at 4 weeks after the oral administration of the test substance. As a result, the expression level of IGF-1 was increased in the test substance Example 3 at 400 mg / kg group compared to the normal group, Expression was low and expression of TGF-β1 was high.

TGF-β1, which is highly expressed in catagen and teolgen, was most frequently expressed in the negative control group. In the test substance group, expression of TGF-β1 was lowered and expression of IGF-1, a hair growth factor, was increased. And induces the factors that convert from the dormant period to the growth period, thereby confirming the effect of promoting hair growth.

In Example 3, 200 mg / kg and Example 3 400 mg / kg of the test substances showed a significant difference in the effect according to the dose. In Example 3, 400 mg / kg of the treated group and 200 mg / And the depth of hair follicles was also increased. According to the above results, the composition according to the present invention can promote hair follicle growth and promote hair growth.

Claims (12)

A green leaf extract, a green leaf extract and a green tea extract,
25 to 75 parts by weight of a self-leaf extract, and 25 to 75 parts by weight of an extract of green tea, based on 100 parts by weight of the perennial herb extract. [Claim 2] The pharmaceutical composition according to claim 1, wherein the extract of Rhododendron japonica extract, Lilium extract and Green tea extract are independently extracted and mixed.
The pharmaceutical composition for preventing or treating hair loss according to claim 1, wherein the perennial herb extract, the self-leaf extract and the green tea extract are not fermented.
The pharmaceutical composition for preventing or treating hair loss according to claim 1, further comprising at least one extract selected from the group consisting of black bean extract, glutinous rice extract, seaweed extract, cinnabar extract, myrtle extract, pungyang extract and licorice extract.
A green leaf extract, a green leaf extract and a green tea extract,
25 to 75 parts by weight of a self-lyophilized extract and 25 to 75 parts by weight of an extract of green tea, based on 100 parts by weight of a perennial herb extract, to prevent or ameliorate hair loss.
[Claim 6] The health functional food for preventing or ameliorating hair loss according to claim 5, wherein the perennial herb extract, the self-leaf extract and the green tea extract are independently extracted and mixed.
The health functional food for preventing or ameliorating hair loss according to claim 5, wherein the Hwasungcho extract, the Japanese lobule extract and the green tea extract are not fermented.
The health functional food for preventing or ameliorating hair loss according to claim 5, further comprising at least one extract selected from the group consisting of black bean extract, glutinous rice extract, seaweed extract, cinnabar extract, haniksikcho extract, Puerariae Radix extract and licorice extract.
A green leaf extract, a green leaf extract and a green tea extract,
25 to 75 parts by weight of a self-leaf extract, and 25 to 75 parts by weight of a green tea extract, based on 100 parts by weight of the perennial herb extract, to prevent or ameliorate hair loss.
[Claim 11] The cosmetic composition according to claim 9, wherein the extract of Rhododendron japonica extract, Lilium extract and Green tea extract are independently extracted and mixed.
[Claim 11] The cosmetic composition according to claim 9, wherein the Hwasungcho extract, Jangsook extract and Green tea extract are not fermented.
[Claim 12] The cosmetic composition according to claim 9, further comprising at least one extract selected from the group consisting of black bean extract, glutinous rice extract, seaweed extract, cinnabar extract, haniksikcho extract, Puerariae Radix extract and licorice extract.

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