KR20160148136A - bodily fluid analyzing apparatus and method of analysing bodily fluid using the same - Google Patents

Abstract

The present invention relates to a body fluid analysis method and a body fluid analysis apparatus using the same.
The present invention provides a method comprising: preparing a specimen comprising an antibody-attached particle that reacts with a target antigen, wherein at least some of the particles include a material that reacts to a magnetic field; Providing the analyte body fluid to the specimen and applying a magnetic field thereto; Irradiating the specimen with light; And receiving light emitted through the analyte body fluid using a photodetector.
The present invention also relates to a sample comprising an antibody-adhering particle which reacts with a target antigen, wherein at least some of the particles comprise a substance which reacts to a magnetic field; A magnetic field generating device for applying a magnetic field to the sample; A light source for irradiating the specimen with light; And a photodetector for receiving the light emitted through the specimen.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a body fluid analysis apparatus and a body fluid analysis apparatus using the body fluid analysis apparatus,

The present invention relates to a body fluid analysis apparatus and a body fluid analysis method using the same.

Currently, the ingredients contained in body fluids are often used as indicators related to health. As an example, the sugar component contained in the blood can serve as an important marker in determining whether or not it is diabetes. Particularly, in the case of suffering from a chronic disease, the health condition can be checked by periodically monitoring the components contained in the body fluids.

On the other hand, the body fluid may contain an infectious component such as various microorganisms, and the health condition may be diagnosed by analyzing an infectious component contained in the body fluid. In the case of conventional immunoassays for screening for infectious agents, a body fluid suspected of microbial infection is collected, cultured to make a colony, and then cultured colonies are subjected to biological or biochemical observation The analysis proceeds in the order. However, in the immunological assays described above, only about 1% of the microorganisms that can be cultured are known to be cultivable in the colony, and the incubation time is at least 2 to 3 days, There is a problem that it is difficult to detect and analyze the real-time unit of an infectious component in body fluids.

Thus, there has been a need for analytical techniques for components of body fluids that can increase detection efficiency, while reducing the effort involved in conventional biological or biochemical observations.

An object of the present invention is to provide a body fluid analyzing apparatus and a body fluid analyzing method capable of accurately and quickly diagnosing constituent components of a body fluid by overcoming the time limit, and having user's convenience of operation.

In order to solve the above-mentioned problems, the present invention provides a method for detecting a target antigen, comprising the steps of: preparing a specimen containing particles having an antibody reacting with the target antigen; Providing an analyte body fluid to the specimen and applying ultrasonic vibration to the specimen; Irradiating the specimen with light; And receiving the light emitted through the analyte body fluid using a photodetector.

Providing the analyte fluid to the specimen may comprise generating a reaction between the antigen in the analyte fluid and the antibody attached to the particle, wherein the reaction between the antigen and the antibody The distribution state of the particles can be changed, or a plurality of particles can be collected around the antigen.

The ultrasonic vibration may be applied in regular or irregular pulses.

Wherein at least some of the particles included in the specimen include a substance that reacts to a magnetic field and applying a magnetic field to the specimen before irradiating the specimen with the analyte fluid after providing the analyte fluid to the specimen And applying the magnetic field may include applying a change in the magnetic field.

Preparing the specimen comprises: preparing particles capable of attaching the antibody; Doping the particle with the material in response to a magnetic field; Preparing said antibody to react with said target antigen; And mixing the particles and the antibody in a solvent.

The step of changing the magnetic field may include the step of applying AC power to the electromagnet generating the magnetic field or the step of relatively translating or rotating or translating and rotating the magnet and the magnetic field generating magnet have.

The peak wavelength of the light and the size of the particle are determined by the shape of the scattered light due to no scattering due to the individual radius of the particle before the antigen antibody reaction and the aspect of the scattered light due to the scattering due to the aggregate radius of the particles after the antigen- It is preferable that the difference is selected to be large.

The method may further include calculating the presence or density of the target antigen in the analyte body fluid based on the information of the light received by the photodetector.

In order to solve the above-mentioned problems, the present invention also provides a test sample comprising particles adhering an antibody reacting with a target antigen; An ultrasonic wave generator for applying ultrasonic vibration to the sample; A light source for irradiating the specimen with light; And a photodetector for receiving the light emitted through the specimen.

The specimen includes a base substrate having a channel for receiving body fluids to be analyzed, and the particles may be disposed on at least a portion of the channel.

The light source may include at least one ultraviolet light emitting diode that provides ultraviolet light having a peak wavelength within a range of 200 to 400 nm.

The photodetector receives the scattered light of the light irradiated to the particle and can be installed at a position where the intensity of the scattered light measured by the difference of the non-scattered light before and after the antigen antibody reaction greatly changes.

Wherein at least some of the particles included in the specimen include a material responsive to a magnetic field and applying a magnetic field to the specimen, wherein the magnetic field generator changes the magnetic field applied to the specimen .

The material responsive to the magnetic field may be ferromagnetic.

The material responsive to the magnetic field may be doped to the particles.

The magnetic field generating device may be an electromagnet.

The magnetic field generator comprises: a magnet; And a moving means provided in at least one of the magnets and the specimen for making the configuration relatively movable with respect to other configurations.

According to the present invention, certain components in body fluids can be quickly and easily identified and analyzed.

In addition, according to the present invention, it is possible to facilitate release of an antigen from a bacterium or a virus contained in a body fluid supplied to a specimen by ultrasonic vibration, and additionally a magnetic field can be added to control the antigen-antibody reaction more actively, .

Also, according to the present invention, the size of the particles reacting with the ultraviolet wavelength can be reduced by providing an ultraviolet ray having a shorter wavelength than the visible ray as a light source. Accordingly, since the number of particles bound by the antibody per unit volume of the specimen can be increased, the analytical sensitivity to the target antigen in the body fluid analysis can be enhanced. In addition, since the ultraviolet light can be easily distinguished from visible light, infrared light and the like existing in a natural environment, there is an advantage that a noise factor due to natural light can be reduced.

In the embodiment of the present invention, an ultraviolet light emitting diode can be applied as an ultraviolet light source. The ultraviolet light emitting diode can provide ultraviolet light having a narrow spectrum half width as compared with an ultraviolet light source such as an ultraviolet lamp, which is advantageous in improving analytical reliability.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a flow chart schematically illustrating a method for analyzing body fluids according to an embodiment of the present invention. FIG.
2A to 2D are diagrams schematically showing a method for analyzing body fluid according to an embodiment of the present invention.
3 is a schematic view showing a method for analyzing body fluid according to another embodiment of the present invention.
4A to 4C are diagrams schematically illustrating a method for analyzing body fluid according to another embodiment of the present invention.
5 is a schematic view of a body fluid analysis apparatus according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. However, the technique of the present invention is not limited to the embodiments described herein but may be embodied in other forms. In the drawings, the width, thickness, and the like of the components are enlarged in order to clearly illustrate the components of each device.

Where an element is referred to herein as being located on another element "above" or "below", it is to be understood that the element is directly on the other element "above" or "below" It means that it can be intervened. In this specification, the terms 'upper' and 'lower' are relative concepts set at the observer's viewpoint. When the viewer's viewpoint is changed, 'upper' may mean 'lower', and 'lower' It may mean.

Like numbers refer to like elements throughout the several views. It is to be understood that the singular forms "a", "an", and "the" include plural referents unless the context clearly dictates otherwise, and the terms "comprise" Or combinations thereof, and does not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

 1 is a flowchart schematically showing a body fluid analysis method according to an embodiment of the present invention.

Referring to FIG. 1, a specimen containing an antibody-attached particle reacting with a target antigen is prepared (Step 110). In one embodiment, the step of preparing the specimen may proceed in the following order.

1. Prepare the antibody to react with the target antigen. As an example, the target antigen may be an infectious component that is present in body fluids and causes various lesions. As an example, the target antigen may be a hospital uniform, such as Salmonella.

2. Next, particles having a predetermined size that can be adhered to a predetermined antibody are prepared. The particles may be made of a polymer material such as polystyrene, polyethylene, latex or the like as an example. The particles may have a diameter of several nanometers to several micrometers as an example.

3. The particles and the antibody are then mixed in a solvent. In the mixing process, the antibody can be adhered to the particles. The antigen accepting portion in the antibody may be disposed on the opposite side of the junction with the particle to achieve an antigen-antibody reaction with the external antigen.

4. Also, in one embodiment, the specimen may be manufactured from the base substrate in the following manner. First, a base substrate having a channel for receiving a body fluid to be analyzed is prepared. The channel may be a region having hydrophilicity with the body fluid to be analyzed in the base substrate. As an example, a barrier may be formed in a region excluding the channel using a resist pattern. The particles are then placed on at least a portion of the channel. The particles may be disposed on the channel to be dispersed on the channel.

Next, the analyte fluid is provided to the specimen (Step 120). The analyte may be, for example, blood, urine, semen, saliva, sweat, and the like. In one embodiment, the analyte fluid may be preliminarily received in a given collection vessel. Subsequently, the analyte fluid may be provided to the specimen by immersing the specimen containing the particles in the collection container. At this time, the analyte fluid may be in contact with the particles on the channel. In another embodiment, the body fluids to be analyzed may be supplied directly to the channel region of the specimen in a syringe. In this case, the analyte fluid may be selectively provided to at least a portion of the channel in which the particles are disposed.

As described above, when the analyte body fluid contacts the particle, the target antigen in the analyte fluid may react with the antibody attached to the particle. When the reaction between the antigen and the antibody proceeds, the distribution state of the particles may change. Specifically, the antigen binds to the antibody by an antigen-antibody reaction, whereby a plurality of the particles to which the antibody binds around the antigen can be collected. Regarding the above reaction pattern, it will be described in detail with reference to FIG.

Next, the specimen is irradiated with light (operation 130). In one example, the light may be visible light, infrared light or ultraviolet light. In one embodiment, the light may be ultraviolet light provided by an ultraviolet light emitting diode. In this case, the ultraviolet ray may have a wavelength of about 200 to 400 nm as an example. The light may be irradiated to the specimen while being modulated to have various wavelengths, or may be irradiated to the specimen in an optically polarized state. The light source may irradiate the particles with the light while scanning the particles at an angle within a predetermined range so that the light is irradiated to the particles at various angles.

Next, light emitted through the body fluid to be analyzed is received using a photodetector (step 140). The light received by the photodetector can be generated by the particle being involved in the incident light. As an example, the light received by the photodetector may be scattered light by the particle, fluorescence by a fluorescent material bound to the particle, or reflected light by the particle. The photodetector may also receive two or more combinations of scattered light, fluorescence, and reflected light described above. Based on the various lights received by the photodetector, the degree of absorption of the incident light by the particles can be calculated.

The photodetector may include, for example, a photodiode, an image sensor, and the like. The plurality of light detectors may be arranged to receive the light at various angles corresponding to the light sources. Alternatively, the photodetector may scan around the specimen, corresponding to the light source, to receive the light at various angles.

Although not shown in the flowchart, the method may further include calculating the presence and density of the target antigen in the analyte body fluid based on the information of the light received by the photodetector.

According to one embodiment, the more active the reaction between the target antigen in the analyte body and the antibody on the particle, the more the plurality of particles can be collected around the antigen. The aggregated particles may increase in diameter as an aggregate. At this time, when light is irradiated from the light source, scattering of light by the aggregated particles can be changed so that scattering characteristics are different from each other when compared with scattering of light when the particles are individually present. That is, as an example, the intensity of the scattered light may increase to correspond to the increased diameter. Therefore, by measuring the intensity of the scattered light, the degree of the reaction between the antigen and the antibody can be predicted.

2A to 2D are diagrams schematically showing a method for analyzing body fluid according to an embodiment of the present invention. Specifically, Figure 2a is a schematic representation of an antibody-affixed particle according to an embodiment of the present invention. Figure 2b is a schematic representation of a specimen comprising particles according to an embodiment of the present invention. Figure 2c is a schematic representation of the reaction mechanism according to an embodiment of the present invention. FIG. 2D is a graph showing scattered light measurement results according to an embodiment of the present invention. FIG.

Referring to FIG. 2A, a particle 210 with an antibody 220 capable of reacting with a target antigen is provided. As an example, the target antigen may be an infectious component that is present in the body fluid to be analyzed and causes various lesions. As an example, the target antigen may be a hospital uniform, such as Salmonella. The particles 210 may be made of a polymer material that can be adhered to the antibody 220. The polymer material may include materials such as polystyrene, polyethylene, and latex. The particles may have a diameter of several nanometers to several micrometers as an example. The method of attaching the antibody 220 to the particles 210 can be accomplished by performing step 110 of FIG. The antigen-accepting portion 222 of the antibody 220 may be disposed on the opposite side of the junction with the particle 210 to achieve an antigen-antibody reaction with the external antigen. As shown, a plurality of antibodies 222 may be attached within one particle 210. The number of antibodies 222 shown is for the sake of convenience only and is not necessarily limited to three.

Referring to FIG. 2B, the specimen 200 has a channel region 230 and a barrier region 240 on a base substrate. The channel region 230 is a region in which a plurality of particles 210 having the antibody 222 of FIG. The channel region 210 may be made of a material having hydrophilicity for the analyte and adhesion to the plurality of particles 210. The barrier region 240 is disposed so as to surround the channel region 210 so that the analyte in the channel region 210 is not released to the outside. As an example, the channel region 210 may be made of a paper material, and the barrier region 240 may be made of a resist material.

Referring to FIG. 2B, the specimen 200 may have a channel inlet 231 through which body fluid is supplied from the collecting container from which the analyte fluid is collected. By immersing the channel inlet 211 in the collection vessel, the body fluid diffuses along the channel and moves into the channel reaction zone 232 where the particles 210 are distributed. An antigen-antibody reaction is performed in the channel reaction region 232 and becomes an analysis target region of the particles 210 due to light emitted from an external light source. An absorption pad 233 is disposed above the channel reaction region 232 to absorb body fluids that are excessively diffused. FIG. 2B shows the shape of the test piece 200 when the test piece 200 is immersed in the collecting container to perform the analyte fluid, but the present invention is not limited thereto. As described above, various other modifications are possible as long as the channel region 230 satisfies the condition capable of accommodating the plurality of particles 210 having the analyte body fluid and the antibody 222. [

As another example, the analyte fluid may be provided to the channel reaction region 232 of the specimen 200 using a syringe. In this case, in the specimen 200, the channel inlet 231 may be omitted.

2C, when the analyte fluid is provided to the channel reaction region 232 in which the particles 210 are distributed, the target antigen 250 in the analyte fluid is separated from the antibody 220 attached to the particle 210 Lt; / RTI > At this time, one target antigen 250 can react with a plurality of antibodies 220. For convenience, the figure shows that one target antigen 250 binds to the antigen-receiving portion 222 of the three antibodies 220. Due to the antigen-antibody reaction, the three particles 210 to which the antibody 220 is attached are collected around the target antigen 250. As described above, the plurality of collected particles can behave as a kind of aggregate having a predetermined radius R2. At this time, the radius R2 as the aggregate may be larger than the radius R1 of the individual particles 210 present.

The graph of FIG. 2 (d) is a result of measuring the scattered light detected through the specimen after irradiating the specimen with a forward scattering angle with respect to the specimen. The first graph 21 in FIG. 2D is a graph when the target antigen is not present in the analyte body fluid, and the second graph 22 is a graph when the target antigen is present in the analyte body fluid. As a specific example, the particle size is about 920 nm, and the experimental result when the wavelength of the light irradiated to the specimen is about 650 nm. In the case of the second graph 22, the aggregation of the particles around the target antigen can follow the form in which three particles are aggregated around one target antigen, as shown in Fig. 2C.

The scattered light thus measured can be scattered by Mie scattering. Non-scattering is electromagnetic scattering due to particles of a generally round shape, which can mean scattering occurring when the particle size is approximately equal to or greater than the wavelength of the incident light. Therefore, only particles having a predetermined size corresponding to the wavelength of incident light can cause non-scattering. As an example, the smaller the wavelength of the incident light is in the ultraviolet region, the smaller the particle size causing non-scattering. The first graph 21 in FIG. 2D is a result of measurement of underexcited light by the single particle 210, and the second graph 22 is a result of measurement of underscore light by the aggregate of three single particles 210 . It is preferable that the difference in the aspect of the scattered light due to no scattering due to the individual radius of the particles before the antigen antibody reaction and the difference in the scattered light due to the scattered scattered light due to the aggregate radius of the particles after the antigen antibody reaction are large.

Referring to the graph, it can be seen that the first graph 21 and the second graph 22 show a tendency that the intensity values of unscattered light are differentiated from each other when the potential scattering angle is between about 40 ° and about 58 ° have. It can be observed that the unscattered intensity value that makes the first graph 21 and the second graph 22 distinguishable from each other can be observed at about 45 °. Thus, the presence or the density of the target antigen can be confirmed in the analyte body fluid through the scattered light measurement result. For example, if the difference in the value of the non-scattering intensity is significant, the density of the target antigen is high, and if the difference in the non-scattering intensity value is weak, the density of the target antigen can be judged to be low.

As a result of the experiment, the inventors confirmed that the antigens contained in the viruses and bacteria contained in the body fluids were accelerated when the ultrasonic vibration was applied to viruses and bacteria.

3 is a diagram schematically showing a body fluid analysis method according to an embodiment of the present invention. Referring to FIG. 3, in comparison with FIG. 2B, another embodiment of the present invention further includes an ultrasonic generator 700 for applying ultrasonic vibration to the test piece 200.

In order to smoothly and smoothly induce the reaction between the antigen and the antibody necessary for the detection of the antigen in the present invention, it is preferable that the antigen is liberated from the virus or bacteria. For this purpose, in the present invention, ultrasonic vibration may be applied to the specimen 200 provided with a body fluid to promote release of the antigen from viruses or bacteria.

Such ultrasonic vibration may be applied continuously to the specimen or intermittently. In addition, even when the vibration is applied in an intermittent pulse shape, the pulse may be regular or irregular. This can also be controlled depending on the manufacturing environment, the manufacturing method, and the state of the base substrate or channel reaction area.

Therefore, when the ultrasonic vibration is applied to the specimen provided with the body fluid as shown in FIG. 3, the antigen-antibody reaction can occur more rapidly because the antigen can be promoted in the channel reaction region.

On the other hand, as a result of experiments and observations made by the inventors, the movement of the antibody attached to the particle caused by an antigen liberated from a virus or a bacterium is caused by a specific Brownian motion, (Fe, Co, Ni, etc.), it is confirmed that the cohesion can be induced more by promoting the Brownian motion by applying a magnetic field from the outside if the material contains a substance which acts sensitively by the magnetic field.

4A to 4C are diagrams schematically showing a body fluid analysis method according to an embodiment of the present invention. Specifically, FIG. 4A is a view schematically showing a state in which all particles having an antibody attached thereto according to an embodiment of the present invention include a substance which is caused to behave by a magnetic field. FIG. 4B is a view schematically showing a state in which a part of particles having an antibody attached thereto according to an embodiment of the present invention includes a substance which is caused to move by a magnetic field. FIG. 4C is a view showing a specimen and a magnetic field generating device including an antibody-adhering particle according to an embodiment of the present invention.

Referring to FIG. 4A, a particle 210 with an antibody 220 capable of reacting with a target antigen is provided. The particles 210 all include a material 212 that reacts to a magnetic field, such as a ferromagnetic material. The ferromagnetic material is added to the particles at a level that is doped into the particles. That is, the ferromagnetic material is added to the particles in a trace amount or a trace amount. This is to prevent the ferromagnet from overcoming the antigen-antibody reaction and being aligned along the magnetic field due to the magnetic field described later. Here, the concentration of doping on the particles of the material sensitive to the magnetic field can be appropriately adjusted depending on the sensitivity of the material 212 to the magnetic field and the degree of Brownian motion of the particles, The manufacturing method, and the state of the base substrate or channel reaction region.

On the other hand, as shown in FIG. 4B, a portion of the particles 210 may be doped with a material 212 reactive to a magnetic field. Even if some particles are doped with a material 212 that reacts with a magnetic field, such particles may act sensitively due to the influence of a magnetic field, so that the material 212 may collide with other undoped particles to induce a reaction .

Referring to FIG. 4C, the specimen 200 has a channel region 230 and a barrier region 240 on a base substrate. The channel region 230 is a region in which the plurality of particles 210 of FIG. 4A are dispersed and arranged or the plurality of particles 210 of FIG. 4B are dispersed and arranged. The channel region 210 may be made of a material having hydrophilicity for the analyte and adhesion to the plurality of particles 210. The barrier region 240 is disposed so as to surround the channel region 210 so that the analyte in the channel region 210 is not released to the outside. As an example, the channel region 210 may be made of a paper material, and the barrier region 240 may be made of a resist material.

Referring to FIG. 4C, a magnetic field generator 600 is additionally positioned adjacent to the specimen 200 as compared to FIG. When the body fluid is supplied to the channel inlet 231 and the body fluid diffuses along the channel to move to the channel reaction region 232 where the particles 210 are distributed, when the antigen-antibody reaction is performed in the channel reaction region 232, When a magnetic field is generated in the generating apparatus 600, some or all of the particles in the channel reaction region are affected by the magnetic field to promote the antigen-antibody reaction.

At this time, it is desirable to give the magnetic field more variably than giving it constantly. In one example, the magnetic field generating apparatus may be an electromagnet, and the electromagnet may be provided with a pulsed direct current power supply or alternatively, a magnetic field may be provided in a volatile manner.

In another example, the magnetic field generating device may be an electromagnet or a permanent magnet, and the magnetic field generating device may be reciprocated in a predetermined section to cause a variation in the magnetic field. Of course, such motion may operate on a magnetic field generator or on a specimen, the specimen may reciprocate and the magnetic field generator may be rotated. In other words, various modifications may be made within a range in which a relative motion is generated in the specimen and the magnetic field generator to cause a change in the magnetic field.

Referring to FIG. 4C, both the ultrasonic generator 700 and the magnetic field generator 600 can operate on the test piece 200. Thus, after the body fluid is supplied to the specimen, both the ultrasonic generator and the magnetic field generator can act on the specimen.

At this time, magnetic field and ultrasonic vibration can be applied to the specimen at the same time. Magnetic fields and ultrasonic vibrations may also be applied alternately to the specimen. Ultrasonic vibration excites the antigen from bacteria and the like, and the magnetic field actively induces the antigen-antibody reaction by further promoting the Brownian motion of the particles attached to the antibody. Therefore, the ultrasonic vibration and the magnetic field are applied to the specimen. It can be determined in an optimal form depending on the kind.

5 is a schematic view of a body fluid analysis apparatus according to an embodiment of the present invention. 5, the body fluid analyzer 400 includes a specimen 410, a light source 420 for irradiating the specimen 410 with light, and a photodetector 430 for receiving light emitted through the specimen 410. [ . The body fluid analyzer 400 also includes a magnetic field generator 600 and an ultrasonic generator 700.

The specimen 410 contains particles attached to an antibody that receives the analyte fluid and reacts with the target antigen in the analyte fluid. The specimen 410 may be the same as the specimen 200 in the above-described embodiment.

The light source 420 may provide the specimen 410 with light having wavelengths in the visible, infrared and ultraviolet regions. In one specific embodiment, the light source 420 may provide ultraviolet light having a wavelength of about 200 to 400 nm. Ultraviolet rays are shorter in wavelength than visible light and ultraviolet rays, and provide greater energy. Accordingly, in the scattered light analysis, it is possible to reduce the size of the particles causing non-scattering and to increase the density of the particles disposed in the channel reaction region. In addition, when ultraviolet rays are used, it is easy to distinguish from visible light and infrared rays in a natural environment at the time of the experiment, thereby generating lower noise.

The light source may also be an ultraviolet light emitting diode. The light emitting diode can be designed to emit light having a narrow half width, which is particularly advantageous when a specific wavelength band is intensively required.

The light source 420 may modulate the generated light to have various wavelengths or change the light to a polarized state. The light source 420 may irradiate the specimen 410 while scanning the specimen 410 at an angle within a predetermined range so as to irradiate the specimen 410 with the light at various angles. Or a plurality of light sources 420 may be disposed so that the emitted light may be irradiated to the specimen 410 at different wavelengths at the same time.

The photodetector 420 may include, by way of example, a photodiode, an image sensor, or the like. The plurality of light detectors may be arranged to receive the light at various angles corresponding to the light sources. Alternatively, the photodetector may scan around the specimen, corresponding to the light source, to receive the light at various angles.

Although not shown, in some other embodiments, the apparatus may further comprise a processor for calculating the presence and density of the target antigen in the analyte based on the information of the light received by the photodetector.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be taken by way of example, It will be understood that the present invention can be changed.

200: The Psalms
210: particles
212: ferromagnet
220: antibody
222: antigen receptor
230: Channel area
231: Channel entrance
232: channel response area
233: Absorption pad
240: barrier area
250: target antigen
400: body fluid analyzer
410: The Psalms
420: Light source
430: photodetector
600: magnetic field generator
700: Ultrasonic generator

Claims (18)

Preparing a specimen comprising particles attached to the antibody that react with the target antigen;
Providing an analyte body fluid to the specimen and applying ultrasonic vibration to the specimen;
Irradiating the specimen with light; And
And receiving light emitted through the analyte body fluid using a photodetector.
The method according to claim 1,
Providing the analyte body fluid to the specimen comprises:
Generating a reaction between the antigen in the analyte body fluid and the antibody attached to the particle,
Wherein the reaction between the antigen and the antibody changes the distribution state of the particles or aggregates a plurality of particles around the antigen.
The method according to claim 1,
Wherein the ultrasonic vibration is applied with regular or irregular pulses.
The method according to claim 1,
Wherein at least some of the particles contained in the specimen comprise a material responsive to a magnetic field,
Further comprising the step of applying a magnetic field to the specimen before irradiating the specimen with the light after irradiating the specimen with the body fluid to be analyzed,
Wherein applying the magnetic field comprises changing the magnetic field.
The method of claim 4,
The step of preparing the specimen includes:
Preparing particles capable of attaching the antibody;
Doping the particle with the material in response to a magnetic field;
Preparing said antibody to react with said target antigen; And
And mixing the particles and the antibody in a solvent.
The method of claim 4,
Wherein the step of applying a change in the magnetic field comprises applying an alternating current power to the electromagnet generating the magnetic field.
The method of claim 4,
The step of imparting a change in the magnetic field comprises relatively translating, rotating, translating, and rotating the specimen with the magnet generating the magnetic field.
The method according to claim 1,
The peak wavelength of the light and the size of the particle are,
Wherein the difference between the pattern of the scattered light due to the non-scattering due to the individual radius of the particle before the antigen-antibody reaction and the pattern of the scattered light due to the scattering due to the aggregate radius of the particle after the antigen-antibody reaction is large.
The method according to claim 1,
Based on information of the light received by the photodetector,
And calculating the presence or density of the target antigen in the analyte body fluid.
A specimen containing particles attached to an antibody that reacts with a target antigen;
An ultrasonic wave generator for applying ultrasonic vibration to the specimen;
A light source for irradiating the specimen with light; And
And a photodetector for receiving light emitted through the specimen.
The method of claim 10,
Wherein the specimen comprises a base substrate having a channel for receiving body fluids to be analyzed, the particles being disposed on at least a portion of the channel.
The method of claim 10,
Wherein the light source comprises at least one ultraviolet light emitting diode that provides ultraviolet light having a peak wavelength within a range of 200 to 400 nm.
The method of claim 10,
Wherein the photodetector receives scattered light of the light irradiated to the particle,
Wherein the intensity of the scattered light measured by the difference in scattering before and after the antigen-antibody reaction is greatly changed.
The method of claim 10,
Wherein at least some of the particles contained in the specimen comprise a material responsive to a magnetic field,
Further comprising a magnetic field generating device for applying a magnetic field to the specimen,
Wherein the magnetic field generating device changes the magnetic field applied to the specimen.
15. The method of claim 14,
Wherein the substance responsive to the magnetic field is a ferromagnetic substance.
15. The method of claim 14,
Wherein the material responsive to the magnetic field is doped to the particle.
15. The method of claim 14,
Wherein the magnetic field generator is an electromagnet.
15. The method of claim 14,
The magnetic field generator comprises:
magnet; And
And a moving means provided in at least one of the magnet and the specimen for making the configuration relatively movable with respect to another configuration.

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