KR20160013435A - Cosmetic composition comprising Juncus effusus extract for improving elasticity of skin and wrinkle - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin elasticity and wrinkle improvement comprising an extract of mustard seed extract as an active ingredient and is excellent in the effect of inhibiting the activity of elastase and collagenase, And exhibits excellent effects on skin elasticity and wrinkle improvement.

Description

[0001] The present invention relates to a cosmetic composition for improving skin elasticity and wrinkle,

The present invention relates to a cosmetic composition for skin elasticity and wrinkle improvement, which comprises an extract of mustard seed extract.

Skin is the primary protective body of the human body. It protects the internal organs from changes in temperature and humidity, ultraviolet rays, pollutants and other external environment, and plays an important role in the maintenance of body homeostasis such as body temperature control. Like other organs in the human body, skin ages gradually as it gets older, resulting in skin elasticity loss, keratinization, wrinkle formation, and skin atrophy as a result of aging.

Skin aging is divided into physiological aging process and aging process by exogenous factor according to time. Among these, ultraviolet rays (UV, ultraviolet) acting as external factors induce various skin troubles and promote aging phenomena such as stain, freckles and skin pigmentation, and cause skin diseases such as skin cancer. In addition, ultraviolet rays decompose collagen and elastin, which are skin elastic fibers, to reduce skin elasticity, causing wrinkles and roughening the surface.

The elasticity of the skin is maintained by the collagen, elastin, hyaluronic acid, etc. of the dermis tissue. Collagen is a major component of the extracellular matrix and is a collagenous fibrous protein that accounts for 90% of the dermis. It forms most of the organic matter in the skin, tendons, bones, and teeth, and the skin's mechanical rigidity, And tissue binding, and is known to support cell adhesion and induce cell division and differentiation. Such collagen is reduced by aging, and reduction of collagen is known to be closely related to wrinkling of the skin. Elastin is a highly elastic protein that occupies 2 to 3% of the dermis and forms a network structure with collagen to give elasticity to the skin. It has strong refraction and stretchability, which increases the skin's elasticity by 1.5 times. . When the elastin is broken down by aging, it is known that the binding of the network structure of the dermis tissue is broken, causing the skin to sag and wrinkle. Both collagen and elastin are produced by fibroblasts.

The function of fibroblasts is regulated by a variety of growth factors, but is also regulated by cytokines secreted by skin cells such as keratinocytes and melanocytes. TGF-beta (transforming growth factor-beta), TGF-beta receptor and SGad3 released from keratinocytes under normal conditions can be obtained from procollagen expressed from dermal fibroblast, Increased expression of fibrillin-1 and tropoelastin increases the production of collagen and elastin and inhibits the expression of MMPs (martrix metalloproteinases) and elastase, resulting in the degradation of collagen and elastin .

In addition to TGF-β, tumor necrosis factor-α, prostaglandin E2, α-MSH, granulocyte stimulating factor (GSF), IL- 1, interleukin-1, IL-6, IL-8, and IL-10. Inhibition of procollagen and tropoelastin expression by acting on fibroblasts and release of IL-1, TNF-α and the like are inhibited by MMP-1 (martrix metalloproteinase-1, "collagenase"), MMP-2, MMP- And promotes the decomposition of collagen and elastin by increasing the activity of elastase and the like. In addition, even if the fibroblasts are directly injured by ultraviolet rays, the collagen and elastin-related genes are suppressed and the expression of the enzymes involved in degradation thereof is promoted and the wrinkles are increased. Therefore, it is possible to suppress the generation of wrinkles by increasing the proliferation of dermal fibroblasts, increasing the synthesis of collagen and the synthesis of elastin, or inhibiting the enzymes that degrade them.

In recent years, herbal medicines have been widely used regardless of the type and formulation of the product, as a cosmetic material having various functions such as whitening, anti-aging, skin improvement, anti-cancer and antibacterial.

A perennial herbaceous plant belonging to the raspberry family, 灯心草 { Juncus effusus There is L. decipiens Buchen . = juncus decipiens ( Buchen .) Nakai refers to the whole grass or stem center of rush and relative plants. It is a lake, an enemy, a sirloin, a jasper, It is also called sirloin, pork loin, and yuzu. It is distributed in Korea, Japan, China, North America, etc. In Korea, it grows in the water and wetlands of the whole country. It has a height of 1.2 ~ 1.5m, a diameter of 2.5mm and has about 10 nodes, and each node has its roots. The epidermis of the stem is a fairytale tissue containing chlorophyll and contains a polysaccharide component. The inner part of the stem contains thick fiber, fatty acid, protein, etc., and it has a long cylindrical shape of noodle shape and outer surface is milky white or pale yellowish white. It has a pressed flat shape and a thin wrinkled shape. The central part of these stems have been used as medicines since ancient times.

The pharmacological actions of rosacea are known as analgesia, diuretic, and hemostatic action. When the urine is not seen in the past, it is known to cause pain, edema caused by pyelonephritis, symptoms of chest tightness and sleepiness, , Sore throat due to pneumonia, and laryngitis.

However, the contents of the skin elasticity and the effect of improving the wrinkles of the sea mustard have not been described or taught.

1. Korean Patent Publication No. 10-2009-0128119 2. Korean Patent Publication No. 10-2014-0047214 3. Korean Patent Publication No. 10-2014-0066844

1. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200. 2. Ficegrini N, Ka R, Yang M, Rice-Evans C. 1999. Screening of diatryl carotenoids-rich fruit extracts for antioxidant activities applying 2,2'-azinobis (3-ethylenebenzeothiazoline-6-sulfonic acid) radical cation decolorization assay . Method Enzymol 299: 379-389. 3. Cannell RJ, Kellam SJ, Owsianka AM, Walker JM. 1988. Results of a large scale screen of microalgae for the production of protease inhibitors. Planta Med 54: 10-14. 4. Wunsch, E., H.G. Heindrich. 1963. Zur quantitativa bestimmung der collagenase. Hoppe Seylers. Z. Physiol. Chem. 333: 149-151. 5. Claude, S., K. Manabu, M. Laura, and P. Lester. 1999. Antioxidants modulate acute solar ultraviolet radiationinduced NKKB activation in a human keratinocyte cell line, Free radical Biol. Med. 26, 174-183. 6. Voegeli, R. 1996. Elastase and typing determination on human skin surface. Cosmetic & Toiletries. 111, 51-58. 7. Jung, H. S., J.H. Ha., Y.K. Kim., S.H. Oh, S.S. Kim., M.H. Jeong., H.Y. Lee. 2009. Effect of Rubus coreanus extracts on ultraviolet-A irradiated cultured human skin fibroblasts. Korean J. Medicinal Crop Sci. 17 (5): 321-327. 8. Gilchrest BA. 1989. Skin aging and photoaging. Journal of the american academy of dermatology. 21: 610-613. 9. Aroca, P., et al. 1993. Melanin biosynthesis patterns of following hormonal stimulation. J. Biol Chem 268, 25650-25655. 10. Chin, J. E., et al. 2005. Effects of Houttuynia cordata extracts on tyrosinase gene expression. J. Korean Soc Food Sci Nutr 34, 1284-1288. 11. Oikarinen A, Karvonen J, Uitto J, Hannuksela M., Connective tissue alterations in skin exposed to natural and therapeutic UV-radiation. Photodermatol. 2 (1), pp. 15-26, Review, 1985. 12. Hirobe T., Role of keratinocyte-derived factors involved in regulating the proliferation and differentiation of mammalian epidermal melanocytes. Pigment Cell Res. 18 (1), pp2-12. Review, 2005. 13. McKay IA, Leigh IM., Epidermal cytokines and their roles in cutaneous wound healing. Br J Dermatol. 124 (6), pp.513-8, Review, 1991. 14. Photoprotective and anti-skin-aging effects of eicosapentaenoic acid in human skin in vivo. Kim, HH, Cho S, Lee S, Kim KH, Cho KH, Eun HC, Chung JH. J Lipid Res. 47 (5), pp.921-30, 2006. 15. Medina A, Ghaffarie, Kilani RT, Ghahary A., The role of stratifin in fibroblast-keratinocyte interaction. Mol Cell Biochem. 305 (1-2), pp. 255-64, Review, 2007. 16. Wang XJ, Han G, Owens P, Siddiqui Y, Li AG., Role of TGF beta-mediated inflammation in cutaneous wound healing. J Investig Dermatol Symp Proc. 11 (1), pp. 112-7, Review, 2006. 17. Traidi-Hoffmann C, MI, Ring J, Behrendt H., Impact of desloratadine and loratadine on the crosstalk between human keratinocytes and leukocytes: Implications for anti-inflammatory activity of antihistamines. Int Arch Allergy Immunol. 140 (4), pp.315-20, 2006.

Accordingly, the inventors of the present invention have completed the present invention by confirming that the extract of Rumex root is superior in the activity of inhibiting elastase and collagenase which reduce the elasticity of skin and promote the generation of wrinkles.

In order to achieve the above object, the present invention provides a cosmetic composition for skin elasticity and wrinkle improvement, which comprises an extract of Seaweed extract as an active ingredient.

It is preferable that the rumen extract is extracted with a solvent selected from water, C ₁ to C ₄ lower alcohols and mixed solvents thereof.

In addition, it is preferable that the composition contains 0.1 to 50% by weight of the above-mentioned Seaweed extract based on the total weight of the cosmetic composition.

The water lanceolate extract of the present invention is excellent in the effect of inhibiting the activity of elastase and collagenase, which decompose elastin and collagen, which are components that maintain elasticity of skin, to reduce elasticity of skin and produce wrinkles. Accordingly, it is possible to suppress the reduction of skin elasticity and the generation of wrinkles caused by aging, and thus it can be effectively used as a cosmetic composition for improving skin elasticity and wrinkles.

FIG. 1 is a graph showing the DPPH radical scavenging activity of the Rumex root extract. FIG.
FIG. 2 is a graph showing the ABTS radical scavenging activity of the water rumen extract.
Fig. 3 is a graph showing the elastase inhibitory activity of the raspberry extract.
Fig. 4 is a graph showing the collagenase inhibitory activity of the raspberry extract.

Hereinafter, the present invention will be described in detail.

One. rush  Preparation of extract

The extraction solvent is added in an amount of about 1 to 5 times, preferably 2 to 4 times the weight of the raw material to the raw beef which is a raw material in a dry state at a temperature of 5 to 100 캜, preferably 20 to 90 캜, more preferably room temperature to 80 캜 For 10 to 30 hours, preferably about 18 to 25 hours, followed by filtration and concentration under reduced pressure to obtain an extract of Seabream extract.

As the extraction solvent, a solvent selected from water, C ₁ to C ₄ lower alcohols and mixed solvents thereof is used, and a mixed solvent of water and ethanol is more preferably used. Examples of the extraction method include a method commonly used in the art such as cold extraction, hot water extraction, ultrasonic extraction, reflux cooling extraction and heat extraction, and it is more preferable to perform cold extraction.

2. For skin elasticity and wrinkle improvement Cosmetics  Preparation of composition

The cosmetic composition for improving skin elasticity and wrinkles of the present invention contains the above sea mustard extract as an active ingredient.

It is preferable that the watercress root extract is contained in an amount of 0.1 to 50% by weight based on the total weight of the cosmetic composition.

The cosmetic composition of the present invention can be prepared in various formulations conventionally produced in the art and examples of the formulations include solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oils, powder foundations, emulsion foundations, wax foundations and sprays, but are not limited thereto. More specifically, the present invention relates to a cosmetic lotion, a lotion, a moisture cream, a nutritional cream, a massage cream, an eye cream, a hand cream, an essence, a pack, a shampoo, a rinse, a soap, a cleansing lotion, a cleansing cream, a cleansing foam, Cleansers, sprays, powders, and the like.

The cosmetic composition may contain, in addition to the above extracts, conventional auxiliaries and carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances.

When the formulation of the present invention is a paste, a cream or a gel, an animal oil, vegetable oil, wax, paraffin, starch, tracer, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide can be used as a carrier component have.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or a polyamide powder can be used as a carrier component. In the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Or propellants such as dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 1,3-butyl glycol, glycerol aliphatic esters, polyethylene glycols or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, the carrier component may include water, a liquid diluent such as ethanol and propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar, tracant and the like may be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, ethoxylated glycerol fatty acid esters, and the like.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples. The following Examples and Experimental Examples illustrate the present invention, and the scope of the present invention is not limited thereto.

[ Example  One]

rush  Preparation of extract

(150 g) purchased from Omni Hub Co., Ltd. (http://www.omniherb.com/) was immersed in 15,000 ml of 75% ethanol at 60 ° C for 24 hours, and then an extract was obtained at room temperature. Then, 15,000 ml of 75% And the extract was further extracted two more times. 10.8 g (yield: 7.2%) of R. cauliflower extract was obtained through concentration and drying at a reduced pressure of evaporating the solvent with a vacuum distillator (Vaccum rotary evaporator, VR-205c, Nihon Seiko, Japan) Respectively.

[ Experimental Example  One]

DPPH Radical  Scavenging activity DPPH radical scavenging activity ) Measure

DPPH is a relatively stable free radical with a deep purple color and exhibits a high absorbance value at 517 nm. When it reacts with a substance having antioxidative effect, it becomes pale yellow and decreases the absorbance at 517 nm. Therefore, it is widely used to search for antioxidants from various materials .

The DPPH radical scavenging activity was determined by using Blois (Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200).

1 ml of 0.2 mM 1,1-diphenyl-2-picrylhydrazyl (DPPH) was added to 2 ml of the sample solution, and the mixture was allowed to stand for 30 minutes, and the absorbance was measured at 517 nm Respectively. For comparison, vitamin C (ascorbic acid) was used in the same experiment.

The DPPH radical scavenging activity was expressed by the absorbance decreasing rate of the sample addition group to which the sample solution was added and the no addition group to which the sample solution was not added as shown in the following Equation 1, and the results are shown in Table 1 and FIG. In Figure 1, JEE represents the jujube extract of the present invention ( Juncus effusus extract) and Vit. C represents vitamin C.

Treated group Concentration (mg / ml) DPPH radical scavenging activity (%) Seaweed extract
0.2 9.96 + 0.10 5 24.50 ± 2.37 Vitamin C 5 76.21 + - 6.05

As can be seen from the results shown in Table 1, the watercress extract of the present invention showed a DPPH radical scavenging activity at a concentration of 5 mg / ml though it was lower than that of vitamin C.

[ Experimental Example  2]

ABTS + Cation Radical  Scavenging activity ABTS + cation radical scavenging activity ) Measure

ABTS is a dark blue-green free radical with a high absorbance value at 732 nm. When it reacts with an antioxidant substance, the absorbance at 732 nm is reduced to near-transparent white, so that antioxidants are detected from various materials It is widely used.

Antioxidant activity using ABTS radicals was measured by ABTS + cation decolorization assay (Fellegrini N, Ka R, Yang M, Rice-Evans C. 1999. Screening of diatry carotenoids-rich fruit extracts for antioxidant activities 2,2'-azinobis (3-ethylenebenzeothiazoline-6-sulfonic acid) radical cation decolorization assay. Method Enzymol 299: 379-389).

A solution of 7 mM 2,2-azino-bis (3-ethyl-benzothiazoline-6-sulfonic acid) {2,2-azino-bis persulfate were mixed and allowed to stand at room temperature for 24 hours to form ABTS + ㆍ. After diluting with ethanol, 100 μl of ABTS + ㆍ 100 μl of sample solution was added and left for 1 minute, and the absorbance was measured at 732 nm. Vitamin C was used for comparison for the same experiment.

The ABTS cation radical scavenging activity was expressed by the absorbance decreasing rate of the sample addition group to which the sample solution was added and the non-addition group to which the sample solution was not added, as shown in the following Equation 2, and the results are shown in Table 2 and FIG. In FIG. 2, JEE represents the Rumex root extract of the present invention and Vit. C represents vitamin C.

Treated group Concentration (mg / ml) ABTS radical scavenging activity (%) Seaweed extract
0.2 18.92 + 0.35 5 96.73 + - 0.84 Vitamin C 5 85.58 ± 1.75

As can be seen from the results of Table 2, the extract of R. codon according to the present invention exhibited a significant ABTS radical scavenging activity even at a concentration of 0.2 mg / ml, and a very high ABTS radical scavenging activity of 96.73% at a concentration of 5 mg / The ABTS radical scavenging activity was much better than that of vitamin C which showed 85.58% scavenging activity.

[ Experimental Example  3]

Elastase ( Elastase ) Inhibition activity measurement

Elastase inhibitory activity Cannell etc. (Cannell RJ, SJ Kellam, Owsianka AM, Walker JM. 1988. Results of a large scale screen of microalgae for the production of protease inhibitors. Planta Med 54: 10-14).

Substrate include N- succinyl - (L-Ala) ₃ -p- nitro not ride {N-succinyl- (L-Ala ) 3 -p-nitroanilide} was used, p is generated from the substrates 20 minutes at 37 ℃ The amount of p-nitroanilide produced was measured at 445 nm.

That is, each test solution was prepared so as to have a constant concentration, 0.5 ml of each test solution was taken in a test tube, and porcine pancreas elastase (2.5 U / ml) dissolved in 50 mM tris-HCl buffer (pH 8.6) (L-Ala) ₃ -p-nitroanilide (0.5 mg / ml) dissolved in 50 mM Tris-HCl buffer (pH 8.6) Respectively. For comparison, the same experiment was carried out using Epigallocatechin gallate (EGCG).

The inhibitory activity of elastase was expressed by the absorbance decreasing rate of the sample addition group to which the sample solution was added and the non-addition group to which the sample solution was not added, as shown in the following Equation 3, and the results are shown in Table 3 and FIG. In Fig. 3, JEE represents the sirloin of the present invention.

Treated group Concentration (/ / ml) Elastase inhibitory activity (%) Seaweed extract 200 104.18 ± 3.48 EGCG 200 52.09 + - 3.66

As can be seen from the results shown in Table 3, the extract of R. codon according to the present invention exhibited an elastase inhibitory activity of 104.18% at a concentration of 200 μg / ml, and an inhibitory activity of elastase which was two times higher than that of EGCG.

[ Experimental Example  4]

Collagenase (collagenase) inhibitory activity is measured

Collagenase inhibitory activity was measured according to the method of Wunsch et al. (Wunsch, E., H. G. Heindrich, 1963. Zur quantitativen bestimmung der collagenase.Hopepe Seylers.Z.Physiol.Chem. 333: 149-151).

That is, the reaction mixture was prepared by adding 4 mM CaCl ₂ to 0.1 M Tris-HCl buffer (pH 7.5), adding 4-phenylazo benzyloxycarbonyl-Pro-Leu 0.15 ml of collagenase (0.5 mg / ml) was added to a mixture of 0.25 ml of the substrate solution and 0.1 ml of the sample solution, and the mixture was allowed to stand at room temperature for 20 minutes After adding 0.5 ml of 6% citric acid, the reaction was stopped and 3 ml of ethyl acetate was added to measure the absorbance at 320 nm. For comparison, the same experiment was carried out using EGCG.

The collagenase inhibitory activity was expressed by the absorbance decreasing rate of the sample addition group to which the sample solution was added and the non-addition group to which the sample solution was not added, as shown in the following Equation 4, and the results are shown in Table 4 and FIG. In FIG. 4, JEE represents the sirloin of the present invention.

Treated group Concentration (/ / ml) Collagenase inhibitory activity (%) Seaweed extract 200 70.38 + - 0.46 EGCG 200 41.89 + - 4.49

As can be seen from the results of Table 4, the extract of R. codon according to the present invention showed a high inhibitory activity of collagenase inhibitory activity of 70.38% at a concentration of 200 μg / ml, which is almost twice that of EGCG.

Claims (3)

A cosmetic composition for improving skin elasticity and wrinkles, comprising an extract of rosemary extract as an active ingredient. The method according to claim 1,
Characterized in that the rumen extract is extracted with a solvent selected from water, C ₁ to C ₄ lower alcohols and mixed solvents thereof. 3. The method according to claim 1 or 2,
Wherein the composition comprises from 0.1 to 50% by weight, based on the total weight of the cosmetic composition.

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