KR20160001102A - Hair cosmetic composition comprising the oriental medicin extract of Graviola for improving state of scalp and preventing hair loss - Google Patents

Abstract

The present invention relates to a hair cosmetic composition comprising a graviolan herbal extract. More particularly, the present invention relates to a hair cosmetic composition comprising a graviolan herbal extract having scalp improvement and hair loss prevention effect.
The hair cosmetic composition for preventing hair loss and scalp according to the present invention is a human-friendly natural cosmetic composition containing graviola water having antimicrobial activity, so that it can be easily used for a person having harmless and sensitive skin to human skin. In addition, it acts effectively to promote hair growth, stimulates scalp hair roots to help prevent hair loss, and also has the advantage of effectively growing hair.

Description

[0001] The present invention relates to a hair cosmetic composition comprising a graviolan herbal extract,

The present invention relates to a hair cosmetic composition comprising a graviolan herbal extract. More particularly, the present invention relates to a hair cosmetic composition comprising a graviolan herbal extract having an effect of improving scalp and preventing hair loss.

The hair normally falls off about 80 ~ 100 per day and new hair is formed in the missing spot. The cause of hair loss varies from person to person, but genetic factors, scalp and health condition and stress are the main causes of hair loss. When blood circulation is good, nutrients and oxygen are supplied smoothly to the hair follicles and hair grows healthy. However, if blood circulation is not smooth due to high fat diet or smoking, hair loss is promoted. In modern society, the number of people who are worried about hair loss is increasing and the age group is getting lower.

Among the conventional hair growth stimulating drugs for treating alopecia, there have been approved drugs for treating hair loss from the US Food and Drug Administration (FDA) such as oral finasteride (MERK, USA) and minoxidil, Upjohn, USA) are known. Minoxidil has a capillary vasodilating function, which is effective against hair loss by applying to the scalp. However, it has to be applied continuously to the scalp. When the medicine is kept on the scalp for a certain period of time, the effect appears and side effects such as itching and irritation are appearing. Pinasteride has the ability to inhibit the production of DHT (5α-dihydrotestosterone), a male hormone that causes hair loss, and has a hair restraining effect when taken over a long period of time. In addition, side effects such as loss of sexual desire and erectile dysfunction, such as erectile dysfunction, and women are at risk of birth defects and should not be taken. That is, since the conventional hair-loss preventive agent is made of a chemical substance, it temporarily expands the capillary blood vessels by circulating blood flow and stimulates the hair follicle, so that the effect is not only temporary but also limited in the effect of hair loss by excessive secretion of sebum There is also.

Recent research reports indicate that when chemically induced oxidative stress is applied to the scalp, hair cycle is significantly shortened and hair loss induced by oxidation of sebum occurs. It is also known that various antioxidants are effective in preventing hair loss and promoting hair growth.

Attempts to prevent hair loss and to promote hair growth have been known to be applied to the scalp using an extract of the herbal medicine component. Korean Patent Publication No. 10-780180 (when using Sukjihuang, Sansui, Pharmacopoeia, Acanthopanax senticosus, Bacillus subtilis and Mulberry), Korean Patent Registration No. 10-1103651 (6 kinds of papaya, Many studies have been conducted to solve the above problems, such as the case of using an extract of oriental herbal medicine) and Korean Patent Registration No. 10-1155502 (when using extracts of phytophthora, licorice, nifty ginseng, ginseng, However, various therapeutic agents for preventing hair loss and improving the scalp have been proposed, but it is not appropriate to fundamentally solve the above problems.

Accordingly, the present inventors have made efforts to develop a novel anti-hair-restoring composition that overcomes the limitations of the prior art, and as a result, they have found that the antimicrobial activity of graviola extract containing an antioxidant phytochemicals component and acetogenin component, Confirming the effect of enhancing cell proliferation and confirming the effect of preventing hair loss and improving the scalp of hair shampoos containing graviola extract, extract of herbal medicine material and copper peptides, and completed the present invention.

Korean Registered Patent No. 10-780180 Korean Patent Publication No. 10-1103651 Korean Patent Publication No. 10-1155502

Blois MS. 1958, Nature, 26, 1199-1120. Roberta R et al., 1999, Free radical biology & Medicine, 26 (9/10), 1231-1237. Stirpe F et al., 1969, J. Biol. Chem., 244 (14), 3855-3863. Marklund S et al., 1974, Eur J Biochem., 47 (3), 469-474. Carmichael J et al., 1987, Cancer Res., 47 (4), 936-942. Yagie et al., 1986, 3981, 517-519. Hosoi J et al., 1985, Cancer Res., 45 (4), 1474-1478.

Thus, it is an object of the present invention to provide a hair cosmetic composition for hair loss prevention and scalp improvement, which further comprises a herbal extract and a capper tripeptide based on graviola extract, and a process for producing the same.

In order to accomplish the object of the present invention, in one embodiment, the present invention provides a method for preparing a cosmetic composition for preventing hair loss and scalp comprising the steps of: 1) dissolving purified water, carbomer, and disodium di ≪ / RTI > and then filtered; 2) dissolving Saccharic acid, Solanum tuberosum extract, glycerin, dimethicone, sodium lauryl sulfate and cocamidopropyl betaine to 70 ° C and mixing and mixing with the filtrate of step 1); 3) dissolving cocamide diame, iodopropionyl butylcarbamate and glycol distearate by heating to 70 ° C, filtering and mixing the product with the product of step 2), and cooling to 60 ° C; 4) Rumonium chloride, fragrance, triethanolamine, sodium chloride, hydrolyzed keratin, dewatering extract, red ginseng extract, white ginseng extract, hanchikcho extract, angelica extract, pomegranate extract, rhubarb extract, capper tripeptide, graviola ethanol extract, Mixing the sodium laureth ether sulfate and the graviola hot water extract with the product of step 3), cooling to 60 ° C and filtering. In the above-mentioned specific example, 13 to 17 parts by weight of purified water, 0.1 to 0.3 parts by weight of carbonon, 0.05 to 0.1 part by weight of disodium iodide, 0.1 to 0.3 parts by weight of cicric acid, 0.1 to 0.3 part by weight of sour pine fruit extract, 1.5 to 2.0 parts by weight of glycerin, 1.5 to 2.5 parts by weight of dimethicone, 8 to 12 parts by weight of sodium lauryl sulfate, 6 to 8 parts by weight of cocamidopropyl betaine, 6 to 8 parts by weight of cocamide dielite, 0.03 to 0.05 part by weight of neo-butylcarbamate, 0.3 to 0.5 part by weight of glycol distearate, 1.8 to 2.2 parts by weight of settimorium chloride, 0.3 to 0.7 part by weight of fragrance, 0.3 to 0.7 part by weight of triethanolamine, , 0.13 to 0.15 parts by weight of red ginseng extract, 0.13 to 0.15 parts by weight of white ginseng extract, 0.13 to 0.15 parts by weight of the extract, 0.13 to 0.15 parts by weight of Angelica gigantosa extract, 0.13 to 0.15 part by weight of the pomegranate extract, 0.13 to 0.15 part by weight of the rhubarb extract, 0.15 to 0.25 part by weight of the caprate tripeptide, 0.4 to 0.6 part by weight of the graviola ethanol extract 23 to 27 parts by weight of sodium laureth sulfate and 23 to 27 parts by weight of a graviola hot water extract. More specifically, 15 parts by weight of purified water, 0.2 part by weight of carborane, 0.2 part by weight of cyclic acid, 0.2 part by weight of sour pine nut extract, 1.7 parts by weight of glycerin, 2 parts by weight of dimethicone, 10 parts by weight of sodium lauryl sulfate, 7 parts by weight of cocamidopropyl betaine 7 parts by weight of cocamide dieye, 0.04 part by weight of iodopropynyl butylcarbamate, 0.4 part by weight of glycol distearate, 0.5 part by weight of fragrance, 0.5 part by weight of triethanolamine, 0.5 part by weight of sodium chloride, 1 part by weight of hydrolyzed keratin, 0.143 part by weight of Sasao extract, 0.143 part by weight of red ginseng extract, 0.143 part by weight of Bleach extract, 0.143 weight part of Angelica giganta extract, 0.143 weight part of pomegranate extract, 0.143 weight part of rhubarb extract, 0.2 weight part of kappa tripeptide, 0.5 weight part of graviola ethanol extract, 25 weight part of sodium laureth sulfate and 25 weight of graviola hot water extract Lt; / RTI > by weight.

Another embodiment of the present invention provides a hair cosmetic composition for preventing hair loss and improving scalp comprising the product prepared in the above embodiment as an active ingredient.

The hair cosmetic composition for prevention of hair loss and scalp according to the present invention is a human-friendly natural cosmetic product, which contains graviola extract having low cytotoxicity and antibacterial activity, and thus can be easily used for a person having harmless and sensitive skin to human skin. In addition, it acts effectively to promote hair growth, stimulates scalp hair roots to help prevent hair loss, and also has the advantage of effectively growing hair.

1 to 4 show the results of measuring antioxidative effects of graviola extract.
FIG. 5 shows the results of measurement of cytotoxicity of graviola extract.
Fig. 6 shows the results of measuring the inhibitory effect of tyrosinase on the graviola extract.
Figure 7 shows the effect of graviola extract on procollagen synthesis.
Fig. 8 shows the results of measuring the antibacterial activity of graviola extract.
Figure 9 shows the effect of graviola extract on the synthesis of antimicrobial peptides.
Fig. 10 shows the results of measurement of the wrinkle-improving effect of gravurea extract by an elastase inhibitor.
Figs. 11 and 12 show the results of measuring anti-inflammatory effects of graviola extract.

Hereinafter, the components and technical features of the present invention will be described in more detail with reference to the following examples. However, the following examples are intended to illustrate the contents of the present invention and are not intended to limit the scope of the invention.

Example

Preparation of extract

1.1 Graviola Ethanol Extract

Graviola leaves and ethanol (70%) were mixed at a weight ratio of 1: 100 to prepare an extract from a 24H rolling extractor. The prepared extract was dried to make powder.

1.2 Graviola hot water extract

Graviola leaves and cosmetics purified water were heated to 100 ° C at a weight ratio of 1: 200 to prepare an extract.

1.3 Single water extract

Ginseng, and ginseng were mixed with butylene glycol and glycerin to prepare an aqueous solution, and the mixture was stirred at 70 ° C to 80 ° C. The mixture was filtered with a 1 mu m filter, allowed to cool, and left at room temperature. After secondary filtration with 0.8 ㎛ and 0.45 ㎛ filters, the preservative was treated and then subjected to tertiary filtration with 0.2 ㎛ filter.

Efficacy test

2.1 Antioxidant effect

1) DPPH radical scavenging ability

DPPH electron donating ability was measured according to the method of Blois (Non-Patent Document 1). To 2 mL of each sample solution containing graviola extract, 1 mL of 0.2 mM 1,1-diphenyl-2-picryl hydrazine (DPPH) was added and stirred. After incubation for 30 minutes, absorbance was measured at 517 nm. The electron donating ability was expressed by the absorbance reduction rate of the sample solution with or without the addition of the sample solution. The DPPH radical scavenging activity of graviola hydrothermal extract and ethanol extract was found to be superior to the positive control vitamin C (Vit-C) at 1,000 ug / ml of graviola ethanol extract (Fig. 1, hot water extract 79% Ethanol extract 91%, vitamin C 83%).

2) ABTS cation radical scavenging ability

The antioxidant activity of ABTS (2,2'-azino-bis (3-ethylbenzthiazoline) -6-sulphonic acid) cation radical was measured by ABTS cation decolorization test (Non-Patent Document 2). (ABTS) and 2.4 mM potassium persulfate were mixed at a final concentration, and the mixture was allowed to stand in a dark place at room temperature for 24 hours to obtain ABTS ⁺ , And diluted with phosphate buffered saline (PBS). After incubation with ABTS ⁺ 100 μL for 1 min, absorbance was measured at 732 nm. As a result of measuring ABTS ⁺ radical scavenging ability of graviola hot water extract and graviola ethanol extract, 500 ㎍ / ㎖ of graviola ethanol extract showed better efficacy than positive control vitamin C (FIG. 2).

3) superoxide anion radical scavenging ability

The superoxide anion radical scavenging activity was measured by NBT (nitrobule tetrazolium) reduction method (Non-Patent Document 3). Add 0.1 mL of each sample solution and 0.4 mL of 0.1 M potassium phosphate buffer solution (pH 7.5) and add 1 mL of a substrate solution containing 0.4 mM of Xanthine and 0.22 mM of NBT. ML) was added to the reaction mixture at 37 ° C for 20 minutes, followed by the addition of 1 mL of 1 N hydrochloric acid to terminate the reaction. The amount of superoxide anion radical produced in the reaction solution was measured by absorbance at 560 nm. As a result of the superoxide anion radical scavenging ability of the graviola hot water extract and graviola ethanol extract, 1,000 ㎍ / ㎖ of graviola hydrothermal extract showed better efficacy than the positive control Vit-C (FIG. 3).

4) superoxide dismutase (SOD) similar bow performance

The SOD-like activity was measured according to the method of Marklund (Non-Patent Document 4). The amount of Tris-HCl buffer solution (50 mM Tris + 10 mM EDTA, pH 8.5) of 2.6 ML and 7.2 mM of pyrogallol was measured at 420 nm in 0.2 mL of each sample solution. SOD - like activity was expressed by the absorbance reduction rate of the experimental solution and the control solution of the sample solution. The efficacies of 10.27% and 22.2% of the extracts of graviola hydrothermal extract and graviola ethanol were observed at 1,000 ug / ml (FIG. 4). The activity of antioxidant enzymes that remove free radicals and toxins in human body was measured and the antioxidant activity was measured as good but the activity of SOD (antioxidant enzyme) was significantly lower than that of vitamin C.

2.2 Cytotoxicity

In the present invention, each cell was cultured in Dulbeco's modified eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) at 37 ° C in a 5% CO2 incubator.

Cell viability was measured according to Camichael's method (Non-Patent Document 5). MTT screening for cell viability is performed using a 96-well plate and an ELISA reader (multiwell microplate reader), which can be used to easily read many samples and is one of the widely used methods for cytotoxicity and cell proliferation detection to be. Each cell melanoma (B16F10), fibroblast (CCRF), and macrophage (Raw264.7) cells were seeded in a 96 well plate with 0.18 mL of 0.6 to 8 × 10 ³ cells / well. , 5% CO ₂ incubator for 24 hours. In the control group, the same amount of sterilized water as the sample was added and the cells were cultured under the same conditions. After adding 0.02 mL of MTT solution (5 mg / mL) for 4 hours, remove the culture medium, add 0.15 mL of DMSO: Ethanol (1: 1) to each well and incubate at room temperature for 30 minutes. Absorbance at 550 nm. The cell viability was measured by the absorbance reduction ratio of the sample solution and the no-added sample solution. MTT assay (B16F10 melanoma) The cell viability was found to be 80% or more at a concentration of 10 ug / ml, and the cell viability of graviola ethanol extract was found to be 80% or more at a concentration of 1 ug / ml (FIG. 5) .

2.3 Whitening effect

1) Insecticidal activity of tyrosinase derived from mushroom

Tyrosinase inhibitory activity was measured according to the method of Yagi et al. (Non-Patent Document 6). To the reaction mixture, 0.2 mL of mushroom tyrosinase (110 U / mL) was added to a mixture of 0.2 mL of the substrate solution in which 10 mM L-DOPA had been dissolved and 0.1 mL of the sample solution in 0.5 mL of 0.175 M sodium phosphate buffer (pH 6.8) For 2 minutes, and the DOPA chrome produced in the reaction solution was measured at 475 nm. The inhibitory effect of tyrosinase was shown by the rate of decrease of the absorbance of the sample solution with and without the sample solution (Fig. 6).

2) Inhibition of melanin biosynthesis in B16F10

Measurement of melanin biosynthesis inhibitory activity was carried out according to the method of Hosoi (non-patent reference 7). The melanoma cells cultured in DMEM medium were divided into 2 × 10 ⁶ cells / dish in a 100 mm culture dish. After culturing for 24 hours, 2 mL of the sample was prepared and the cells were washed with PBS (pH 7.4) 48 hours later . The cells were then desalted with 0.25 M trypsin-EDTA solution and harvested. The cells were treated with 1 mL of 5% TCA per 1 × 10 ⁶ cells, centrifuged twice at 2,500 rpm, and the isolated melanin was washed with PBS Then, 1 mL of ether: ethanol (1: 3) was added, and the mixture was centrifuged two times, washed with 1 mL of ether and dried. 1 mL of 1 N NaOH was added to the dried melanin, reacted at 80 ° C for 1 hour, and the absorbance was measured at 405 nm using a spectrophotometer. The inhibition of melanin biosynthesis was expressed by the absorbance reduction rate of the addition group of the sample solution and the no addition group.

3) Pro-collagen synthesis rate

The effect of graviola extract on procollagen synthesis was measured (Fig. 7).

2.4 Antimicrobial activity

The nutrient broth (NB) was used as a liquid medium for Staphylococcus epidermidis (Escherichia coli) and Escherichia coli (E. coli) as a liquid medium for pre-culture and main cultivation, tryptic Soy broth (TSB) was used, and Propionibacterium acnes (P. acnes) used Gifu anaerobic medium (GAM) medium. S. epidermidis and E. coli were cultured using nutrient agar (NA), S. aureus was cultured using tryptic soy agar (TSA), and P. acnes was cultured in Gifu anaerobic medium (GAM ) Agar was added to the medium and used in this experiment. P. acnes was incubated at 37 ° C in a 5% CO 2 incubator, and all other strains were incubated at 37 ° C in a BOD incubator.

The antibacterial activity of the graviola hot water extract was tested. Regular purified water was used as a control. Staphylococcus aureus and E. coli were cultivated in a medium containing graviola water and general purified water for 4 weeks to confirm the antibacterial activity. The antimicrobial activity was measured by paper disc method. Each strain cultivated in a single colony on a plate medium was inoculated in 10 mL of a liquid culture medium for 0.1 to 3 mL for 3 to 6 hours, The cells were inoculated at a density of about 1 x 10 < 7 > cells per plate medium and homogeneously plated with a sterilized cotton swab. The sterilized filter paper discs (8 mm, Whatman, Japan) were placed on a solid plate medium and then absorbed at a concentration of 50 μL / disc and incubated at 37 ° C for 18-24 hours to obtain clear zone (mm) (Fig. 8).

2.5 Expression of Antimicrobial Peptides

After treatment of graviola extract with human keratinocyte, the expression of antimicrobial peptides CAMP (Cathelicidin antimicrobial peptide) and LL-37 expressed in skin cells themselves was confirmed. Cell line (B16F10, CCRF, Raw246.7) was cultured in tissue culture dish for 24 hours to confirm MITF, TRP-1, TRP-2, MMP-1, iNOS and COX- Stabilized. After the medium was removed, the sample was incubated in a concentration-treated medium for 24 to 48 hours. Then, the medium was removed again and washed twice with PBS. To 10 ml of RIPA buffer, complete mini 1 tab was added and dissolved in 100 μl, and centrifuged at 4 ° C and 12,000 rpm for 20 minutes. The proteins obtained by centrifugation were quantified by Bradford assay using bovine serum albumin (BSA) as a standard and electrophoresis at 120 V for 1 hour using 10% SDS-polyacrylamide gel. After loading, polyvinylidenedifluoriden (PVDF) was used and transferred at 30 ~ 40 V for more than 2 hours. At the end of the transfer, the pancreas was immersed in ponceau S and the band was confirmed. After washing twice with TBST, the skin was overlaid with 5% skin milk to remove the background. After washing three times, the primary antibody (1: 1000) was stuck for 1 hour, and then the secondary antibody (1: 1000) was added and transferred to a film using an ECL kit (Amersham Pharmacia, England). The band density was confirmed by Gel doc (Bio-rad, America) (Fig. 9).

2.6 Wrinkle-reducing effect

The wrinkle-reducing effect of the graviola extract was measured by measuring the elastase inhibitory activity. Elastase inhibition assay was performed according to Cannell et al. (Non-patent reference 7). The amount of p-nitroanilide produced from the substrate for 20 min at 37 ° C was measured at 405 nm using N-succinyl- (L-Ala) 3-p-nitroanilide as a substrate. 0.5 mL of porcine pancreas elastase (2.5 U / mL) dissolved in 50 mM tris-HCl buffer (pH 8.6) was added to each test solution, and 50 mM N-succinyl- (L-Ala) 3-p-nitroanilide (0.5 mg / mL) dissolved in tris-HCl buffer (pH 8.6) was added and reacted for 20 minutes. The elastase inhibitory activity was expressed by the absorbance decreasing rate of the sample solution added group and the no-added group (FIG. 10).

2.7 Anti inflammatory effect

Nitric oxide (NO) measurement was carried out by measuring the amount of NO in the supernatant of the cell as nitrite and nitrate (Non-Patent Document 8). Griess reagent (Sigma, USA), a safe form after nitrite reduction to nitrite, was used. In a 6-well plate, 2 × 106 cells were washed twice with PBS at 80% confluence, (LPS) (10 μg / mL) was added to all wells except the control group for stimulation. After 2 hours, the samples were treated by concentration. The amount of NO produced after 24 hours was measured by absorbance at 540 nm after collecting the supernatant and reacting for 10 minutes with griess regent (FIG. 11). In addition, the inhibitory effect of graviola extract on iNOS expression was measured (Fig. 12).

Hair Cosmetics  Preparation of composition

1) Carbomer (0.2 part by weight) and disodium iodide (0.07 part by weight) were added to 15 parts by weight of purified water and heated to 90 占 폚 to dissolve the hair cosmetic composition according to 100 parts by weight of the hair cosmetic composition according to the present invention. The liquor was filtered; 2) 0.2 part by weight of citric acid, 0.2 part by weight of sour pine nut extract, 1.7 parts by weight of glycerin, 2 parts by weight of dimethicone, 10 parts by weight of sodium lauryl sulfate and 7 parts by weight of cocamidopropyl betaine were mixed, Warmed to dissolve and mixed with the filtrate of step 1); 3) 7 parts by weight of cocamide diad, 0.04 parts by weight of iodopropynyl butylcarbamate and 0.4 parts by weight of glycol distearate were dissolved by heating to 70 ° C, filtered and mixed with the product of step 2) Gt; 60 C < / RTI > 4) Tweezers of settimonium chloride, 0.5 parts by weight of fragrance, 0.5 parts by weight of triethanolamine, 0.5 parts by weight of sodium chloride, 1 part by weight of hydrolyzed keratin, 0.143 parts by weight of Sasao extract, 0.143 parts by weight of red ginseng extract, 0.143 part by weight of the myrtle extract, 0.143 part by weight of the myrtle extract, 0.143 part by weight of the prawn extract, 0.143 part by weight of the prawn extract, 0.143 part by weight of the rhododendron extract, 0.2 part by weight of the caplet tripeptide, 0.5 part by weight of graviola extract, And 25 parts by weight of purified water substitute (graviola water) were mixed and stirred with the mixture cooled to 60 ° C in step 3), cooled to 35 ° C, and filtered to prepare a hair cosmetic composition.

Claims (4)

A method for producing a cosmetic composition for hair loss prevention and scalp comprising the steps of:
1) dissolving in purified water, carbomer and disodium edithio by heating to 90 ° C and then filtering;
2) dissolving Saccharic acid, Solanum tuberosum extract, glycerin, dimethicone, sodium lauryl sulfate and cocamidopropyl betaine to 70 ° C and mixing and mixing with the filtrate of step 1);
3) dissolving cocamide diame, iodopropionyl butylcarbamate and glycol distearate by heating to 70 ° C, filtering and mixing the product with the product of step 2), and cooling to 60 ° C;
4) Rumonium chloride, fragrance, triethanolamine, sodium chloride, hydrolyzed keratin, dewatering extract, red ginseng extract, white ginseng extract, hanchikcho extract, angelica extract, pomegranate extract, rhubarb extract, Sodium laureth ether sulfate and graviola hot water extract are mixed with the product of step 3), cooled to 60 ° C, and filtered.
The method according to claim 1,
0.1 to 0.3 parts by weight of purified water, 0.1 to 0.3 parts by weight of carborane, 0.05 to 0.1 part by weight of disodium dithiae, 0.1 to 0.3 part by weight of cicric acid, 0.1 to 0.3 part by weight of sour pine fruit extract, 1.5 to 2.5 parts by weight of dimethicone, 8 to 12 parts by weight of sodium lauryl sulfate, 6 to 8 parts by weight of cocamidopropyl betaine, 6 to 8 parts by weight of cocamide diisocyanate, 0.03 of iodopropynyl butyl carbamate 0.3 to 0.7 parts by weight of triethanolamine, 0.3 to 0.7 part by weight of sodium chloride, 0.3 to 0.7 parts by weight of sodium chloride, 0.3 to 0.5 parts by weight of glycerol distearate, 1.8 to 2.2 parts by weight of settimorium chloride, 0.3 to 0.7 parts by weight of fragrance, 0.13 to 0.15 parts by weight of a red ginseng extract, 0.13 to 0.15 parts by weight of a white ginseng extract, 0.13 to 0.15 parts by weight of a ginseng extract, 0.13 to 0.15 parts by weight of a red ginseng extract, 0.13 to 0.15 part by weight of the Angelica giganta extract, 0.13 to 0.15 part by weight of the Angelica giganta extract, 0.13 to 0.15 part by weight of the pomegranate extract, 0.13 to 0.15 part by weight of the rhubarb extract, 0.15 to 0.25 part by weight of the kappa tripeptide, 0.4 to 0.6 part by weight of the graviola ethanol extract, 23 to 27 parts by weight of ether sulfate and 23 to 27 parts by weight of graviola hot water extract.
The method according to claim 1,
15 parts by weight of purified water, 0.2 part by weight of carbonara, 0.07 part by weight of disodium diteary, 0.2 part by weight of cyclic acid, 0.2 part by weight of sour pine nut extract, 1.7 parts by weight of glycerin, 2 parts by weight of dimethicone, 10 parts by weight, cocamidopropyl betaine 7 parts by weight, cocamide dieye 7 parts by weight, iodopropynyl butyl carbamate 0.04 part by weight, glycol distearate 0.4 part by weight, settimorium chloride 2 parts by weight 0.5 part by weight of fragrance, 0.5 part by weight of triethanolamine, 0.5 part by weight of sodium chloride, 1 part by weight of hydrolyzed keratin, 0.143 part by weight of sulfoisophthalate extract, 0.143 part by weight of red ginseng extract, 0.143 part by weight of blanched extract, 0.143 weight part of Angelica giganta extract, 0.143 weight part of pomegranate extract, 0.143 weight part of rhubarb extract, 0.2 weight part of capper tripeptide, 0.5 weight part of graviola ethanol extract, 25 parts by weight of leucose and 25 parts by weight of graviola hot water extract.
A hair cosmetic composition for hair loss prevention and scalp, which is produced by the method according to any one of claims 1 to 3.

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