KR20130083570A - A composition comprising an extract of rhus vernicifluastokes for the prevention and treatment of arthritis - Google Patents

Abstract

The present invention relates to a composition for the prevention and treatment of degenerative arthritis containing Rhus verniciflua Stokes extract as an active ingredient, the lacquer hydrothermal extract is effective for the growth and regeneration of activated cells and the generation of cartilage in cells. It provides toxic and antioxidant effects and can be useful as a composition for the prevention and treatment of degenerative arthritis and health functional food containing lacquer extract with excellent cell death suppression effect as an active ingredient.

Description

A composition comprising an extract of Rhus vernicifluaStokes for the prevention and treatment of arthritis}

The present invention relates to a composition for the prevention and treatment of degenerative arthritis containing Rhus verniciflua Stokes extract as an active ingredient, the lacquer extract of the present invention is effective in the growth and regeneration of activated cells and cartilage production in cells. It provides a cytotoxic and antioxidant effect, and provides a composition for the prevention and treatment of degenerative arthritis, which contains the lacquer extract excellent in inhibiting cell death as an active ingredient.

Rhus verniciflua Stokes is a deciduous broad-leaved arborescent belonging to the family Anacardiaceae, and is known for its origin in the Central Asian Highlands and the Himalayas, and is widely distributed in the subtropical and temperate regions of the world. Deciduous or evergreen, mostly arborescent or shrub but partly vine-grown (Barkley Fred Alexander., Ann. Of the Missouri Bot. Garden., 24 (3), pp 265-500, 1937).

Specifically, the stem of the sumac is straight up, the leaves are bizarre, spiny, and many leaves symbiotic with one leaf, including 25 ~ 40cm including the tip and the end of the leaf.

Small leaf is 7-20cm, 3-6cm wide egg shape or round egg shape 9-13. Flowers are male and female, light greenish yellow, pointed, round, flowering, splattered, 15-25cm long. Fruits are spherical, 6-8 mm in diameter, ripen in light yellow in October.

The sap of lacquer tree is called lacquer, and lacquer is used as lacquer for painting and industrial purposes. In oriental medicine, it has been used for insect repellent, abdominal pain, tuberculosis, constipation, fish blood, woman meridian. It can be used as a special exterior paint used for submarine fiber optic cables. Moreover, oriental medicine has been used for a long time, and oriental medicine has been introduced in the treatment of internal tumors and women's menstrual disorders, gastrointestinal drugs, repellents, blood circulation and anti-aging folk remedies (Hota, M. 1989). World Useful Plant Dictionary, Common History (Japan) 910-2). In particular, in Korea, lacquer trees have been used for lacquer chickens, lacquer ducks, etc. (New Testament, Il-hoon Kim, Publisher: Namu, 1986). However, for those who are sensitive to lacquer, dermatitis and itching may be caused by very little contact with lacquer, and most people tend to be reluctant to make contact with lacquer. About 3 to 4 of them.

Even after allergic to lacquer temporarily, the sensitivity is extremely high, and even a small amount of contact can cause symptoms of dermatitis.

The main component of the lacquer sap (Lac lacquer) is urushiol (Urushiol), its main purpose is used in lacquerware, and its appearance is a dark brown liquid, which dissolves well in organic solvents. The chemical structure is mainly composed of 15 to 17 carbon atoms in the side chain, and many unsaturated bonds exist.

The main pharmacological effects of lacquer are known to be anti-cancer, antioxidant, hangover-relieving action (Food Science Journal, 31, 1997, 238-245), gastrointestinal disease, heart disease, arthritis, high blood pressure, diabetes, stroke, chronic fatigue, etc. Known. Despite these various pharmacological actions, it has been known that the commercial use is not due to allergic properties to the human body.

According to the recent research results, lacquer solution is the main ingredient of lacquer liquid and allergic lactate component has strong anti-cancer, antioxidant and antimicrobial activity, and the flavonoids extracted from the bark and the neck of lacquer tree show the inhibitory effect on angiogenesis. The anti-cancer effect of inhibiting the proliferation and metastasis of cancer cells and inducing the differentiation of cancer cells into normal cells was also confirmed. It is a powder made by crushing the lactose into powder and it is used in oriental medicine and private medicine to promote blood, excessive acid, dysmenorrhea, fish blood removal, fundominitis, and insect repellent.

Until now, lacquer tree is mainly in the form of hot water obtained by heating such as chicken, rabbit, duck, etc. In this case, allergy occurs after ingestion due to urushiol, an allergen that is present in the lacquer tree. There has been and has been eating it simply because it is good for the body.

Development of a method that can be ingested without side effects while maintaining the pharmacological effects of sumac has been required for the public's health recovery and health promotion, and some technologies have been developed. The present inventors and applicants continue to push forward the research of the sumac until now.

In general, degenerative arthritis is one of the most common arthritis diseases, in which articular cartilage surrounding the joint surface of the bone is worn and bones under the cartilage are exposed, and inflammation and synovial membrane around the joint cause pain and deformation. to be.

The causes of joint cartilage damage may be caused by overweight, trauma of the joint, disease of the surrounding bone, muscle weakness, nerve damage of the joint, or other genetic predisposition. It also occurs a lot and can also occur on fingers or toes.

As a treatment, acetoaminophen or tramadol may be used as a non-narcotic analgesic to simply relieve pain. Aspirin may be used as a nonsteroidal anti-inflammatory agent in swollen joints. Ointments such as ibuprofen, indomethacin, and naproxine, which are not controlled with acetoaminophen or are used to reduce inflammation of inflammatory degenerative arthritis, are used as ointment analgesic agents or trasts, ketotops, rheumatoids that are applied directly to the skin. It uses patch-type painkillers such as Stop, and intraarticular injection therapy with corticosteroids and hyaluronan for degenerative arthritis that does not respond to medication.

In recent years, cartilage components (glucosamine and chondroitin) have emerged as therapeutic agents. In Europe, they are classified as drugs and used in actual patients.

Physiotherapy, along with medications, is very helpful in relieving your symptoms, and strengthening the muscles around your joints is important not only to relieve your symptoms, but also to prevent exacerbations of osteoarthritis. Therefore, rather than relying solely on medications, you can expect the best effect only with physical therapy. In the early stages, you need to get physical therapy with the help of a specialist and learn how to do it yourself.

If the above methods do not have a great effect, surgery will be considered. Surgery may be done using arthroscopy or osteotomy, which allows bones to be shaped correctly for people who are relatively active. In addition, articular cartilage can be transplanted, but transplantation is performed only when there is an abnormality in the local area. Therefore, this is not the case for degenerative arthritis, in which cartilage wear is widespread. If arthritis is so severe that other options do not heal or are likely to heal, arthroplasty is performed.

Therefore, the present inventors pharmacologically observed the immunosuppressive effect, synovial cell activity suppression effect, anti-inflammatory effect and preventive and therapeutic effects in arthritis animal models of the lacquer extract while studying the efficacy of preventing and treating degenerative arthritis of the lacquer extract. As a result, it was confirmed that the degenerative arthritis prevention and treatment effect is excellent to complete the present invention.

[Related Technical Literature]

1. A pharmaceutical composition for preventing or treating arthritis containing ginseng extract (patent application No. 10-2004-0084427)

In the present invention, the lacquer extract of the present invention is effective in the growth and regeneration of activated cells and cartilage production in cells, provides cytotoxicity and antioxidant effect, and degenerative arthritis containing lacquer extract with excellent cell death suppression effect as an active ingredient. It relates to a composition for the prevention and treatment of.

An object of the present invention is to provide a pharmaceutical composition and health functional food for the prevention and treatment of degenerative arthritis containing the extract of Rhus verniciflua Stokes as an active ingredient.

According to a first aspect of the present invention for achieving the above object, the lacquer extract obtained by dipping C1-4 lower alcohol, ethanol alcohol after hot water extraction and C1-4 lower alcohol, alcohol alcohol, acetone extraction or hot water extraction It provides a composition for the prevention and treatment of degenerative arthritis comprising as an active ingredient.

According to a second aspect of the present invention for achieving the above object, Fustine (3,3 ', 4', 7) having C ₁₅ H ₁₂ O ₆ (molecular weight 288) and white crystals fractionated with Sumac hot water extract -tetrahydroxyflavanone), C ₁₅ H ₁₀ O ₆ (molecular weight 286), yellow crystals, fisetin (3,3 ', 4', 7-Tetrahydroxyflavone), C ₁₅ H ₁₀ O ₅ (molecular weight 272), thick Sulphurtin with orange crystals (3 ', 4', 6'-Trihydroxyaurone), C ₁₅ H ₁₂ O ₅ (molecular weight 272) and orange crystals Butane (2 ', 3,4, 4'-Tetrahydroxychalcone), Garbanzol with C ₁₅ H ₁₂ O ₅ (molecular weight 272), Quercetin with C ₁₅ H ₁₀ O ₇ (molecular weight 302), 5-deoxykaempferol with C ₁₅ H ₁₀ O ₅ (molecular weight 270), C ₆ H ₆ O ₂ (molecular weight 110) Resorcino, C ₇ H ₆ O ₄ (molecular weight 154) 3,4-Benzoic acid, C ₇ H ₆ O ₄ (molecular weight 154) 2,4-Benzoic acid, C ₇ H ₆ Gallic acid with O ₅ (molecular weight 170), Caffeic acid with C ₉ H ₈ O ₄ (molecular weight 180), C ₉ H ₁₁ O ₄ N ₅ (molecular weight 253) provides a composition for the prevention and treatment of degenerative arthritis containing a single component of Adenosin, C ₆ H ₅ O ₂ N (molecular weight 123) Niacin.

At this time, according to an additional feature of the present invention, the growth and regeneration of the cell is preferably set to 300ug / ml concentration of the lacquer extract and 100ug / ml concentration of the lacquer extract to control gene expression.

It may also be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, oral formulations of aerosols, external preparations, suppositories and sterile injectable solutions according to conventional methods of preparation.

In addition, health functional foods for improving arthritis, which contains the lacquer extract as an active ingredient, which suppresses the proliferation of immune cells and synovial cells, provides cytotoxic and antioxidant effects, and has the effect of inhibiting cell death as an active ingredient. To provide.

In the composition for the prevention and treatment of degenerative arthritis containing the lacquer extract according to the present invention as an active ingredient, the lacquer extract has an effect on cell growth and regeneration according to the expression of genes related to cartilage production in cells.

In addition, the application of the present invention is not limited to the matters mentioned in the above examples, the lacquer extract powder, granules, tablets, capsules, suspensions, emulsions, syrups, oral formulations of aerosols, external preparations, suppositories and sterilization It can be formulated in various forms in the form of injectable solutions, and can be formulated as a health functional food by mixing with a carrier of a conventional health food.

1 is a flow chart showing a process for producing a powder of the sumac hot water extract according to the present invention.
2a to 2b is a chemical structural formula of the fractionated single substance of the lacquer extract according to the present invention
Figure 3 is a graph showing the results of cytotoxicity of the lacquer extract in the Multiple Range test of chondrocytes determined by MTT assay ANOVA of the lacquer extract according to the present invention.
Figure 4 is a graph showing the effect of protecting or inhibiting chondrocytes from apoptosis induced by H ₂ O ₂ in the sumac extract according to the present invention.
Figure 5 is a graph showing the evaluation of chondrocyte activity of the effect of lacquer hot water extract on the incorporation for intracellular proteoglycan synthesis of isotope labeled sodium sulfate of the lacquer extract of the lacquer tree extract according to the present invention.
6 to 7 are graphs showing that the production of Collagen and Aggrecan increases as a result of performing Real Time PCR of the extract of Sumac according to the present invention.
8 to 9 is a graph showing the reduced state of the MMPs of the lacquer extract according to the present invention MMPs measurement results.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

The sumac extract according to the present invention uses the sumac extract extracted by a conventional extraction method.

Hereinafter, the present invention examines the effects on chondrocytes isolated from male SD rats using the lacquer hydrothermal extract as a sample in two aspects: 1) cell growth and regeneration, and 2) expression of genes involved in cartilage production in cells. The cell growth and regeneration were selected to be effective at 300ug / ml concentration of the lacquer hot water extract, and the effect of the extract was adjusted to the conditions at 100ug / ml concentration to control gene expression. I would like to.

1. Experimental Method

1.1 Separation of substances from sumac

(Step 1) Preparation of Sumac Extract Powder

As shown in Figure 1, in order to obtain the lacquer extract powder, after cutting the wood part of the lacquer and dried for about one week to three months in the shade, it is cut into 5cm to 15cm length and used to produce wood chips or sawdust. Put drinking water of 10 times the weight of sumac and extract twice at 80 to 120 ℃ for 4 hours.

Alternatively, methanol, spirit alcohol and the like are added in the same amount as a solvent and extracted at a temperature of 20 to 60 ° C. The same amount of acetone may be added and left for 1 to 10 days in a water bath at 45 ° C. at 45 ° C. or for 10 to 30 days in a room to obtain a yellow extract.

The alcohol obtained by immersing the C1-4 lower alcohol and alcohol alcohol in the extract obtained by extracting the lacquer as described above is dried by hot air drying, freeze drying or vacuum drying.

In this case, the C1-4 lower alcohol may be used as methanol, ethanol in the form of alcohol, or alcohol alcohol.

In the extraction process of the lacquer extract powder, the wood part of the lacquer is cut in the extraction process and dried for about 1 month in the shade, or it may be cut and used for 10 cm in length. have.

In addition, the same amount of acetone may be left for 5 days in a water bath at 40 ℃ or left for 10 to 30 days in the room to obtain a yellow extract.

(Step 2) Material Separation Process of Sumac

The extract of the lacquer tree obtained in the above (step 1) is partitioned with hexane to separate the nonpolar portion, and the remaining aqueous layer is partitioned with ethyl acetate and concentrated under reduced pressure. The hexane layer, ethyl acetate layer, and water layer partitioned by polarity were separated into three fractions by silica gel, and then separated by effective column chromatography using several column chromatography.

Separation of fractions and materials was carried out using various chromatographic methods such as sephadex LH-20, ODS-A, diol, preparative MPLC, or preparative HPLC as well as silica gel.

The lacquer hot water extract according to the present invention is divided into three fractions, and the extract of the lacquer tree is partitioned according to the polarity, and each fraction is separated by using various columns including silica gel, using 1D NMR and 2D NMR methods. Was identified and structural confirmation of 14 single materials was completed using modern instruments such as LC / MS, UV, IR.

The 14 monomaterials fractionated by the above procedure have the chemical formulas shown in FIGS. 2A-2B and each molecular formula is C ₁₅ H ₁₂ O ₆ (molecular weight 288), with fustine having white crystals ( 3,3 ', 4', 7-tetrahydroxyflavanone), C ₁₅ H ₁₀ O ₆ (molecular weight 286) and yellow crystals of fiscetin (Fisetin; 3,3 ', 4', 7-Tetrahydroxyflavone), C ₁₅ H Sulphurtin (3 ', 4', 6'-Trihydroxyaurone) with ₁₀ O ₅ (molecular weight 272) and dark orange crystals, butane with C ₁₅ H ₁₂ O ₅ (molecular weight 272) and orange crystals Butein; 2 ', 3,4,4'-Tetrahydroxychalcone), Garbanzol with C ₁₅ H ₁₂ O ₅ (molecular weight 272), Quercetin with C ₁₅ H ₁₀ O ₇ (molecular weight 302), C ₁₅ H ₁₀ O ₅ (molecular weight 270 ) 5-deoxykaempferol, C ₆ H ₆ O ₂ (molecular weight 110), C ₇ H ₆ O ₄ (molecular weight 154) 3,4-Benzoic acid, C ₇ H ₆ O ₄ (molecular weight 154) 2, 4-Benzoic acid, C ₇ H ₆ O ₅ (molecular weight 170) Gallic acid, C ₉ H ₈ O ₄ (molecular weight 180) affeic acid, Adenosin with C ₉ H ₁₁ O ₄ N ₅ (molecular weight 253) and Niacin with C ₆ H ₅ O ₂ N (molecular weight 123).

1.2 Materials and Methods

Name Company Catalog Number One FBS Hyclone, USA SH30396.03 2 Penisilin Streptomycin Hyclone, USA SV30010 3 L-glutamine Hyclone, USA SH30034.01 4 Trypsin Hyclone, USA SH30042.01 5 DMEM Hyclone, USA SH30243.01 6 Gentamycin sulfate Lonza, USA 17-518Z 7 DPBS Hyclone, USA SH30028.03 8 Thiazolyl Blue Tetrazolium Bromide Sigma, USA M5655 9 Dimethyl sulfoxide Sigma, USA D4540 10 Collagenase Type I Sigma, USA C0130 11 Ethylenediaminetetraacetic acid (Disodium Salt: Dihydrate) Sigma, USA E5134 12 Recombinant RNasin Ribonulease Inhibitor Promega, USA N2515 13 AccessQuick RT-PCR System Promega, USA A1702 14 IQ SUBR Green Supermix BIO-RAD 170-8880 15 iScript Select cDNA Synthesis Kit BIO-RAD 170-8897

Table 1. Key Reagents and Materials

1.3 Isolation and Culture of Chondrocytes

The experimental chondrocytes were obtained from rat primary culture, and the primary culture proceeded as follows.

The experimental animals for cartilage were collected by disinfecting the skin with 70% alcohol after cervical dislocation after male Sprague-Dawley rats weighing about 180-200g weighing 4 to 6 weeks. .

The collected cartilage tissue was stored in phosphate buffer saline (calcium & magnesium free, Hyclone SH30256.01) solution, transferred to a clean bench, and incubated twice with 0.1% EDTA-CDMF (calcium magnesium free, Sigma E5134) solution for 30 minutes. 37 ° C., 5% CO 2). Incubation with 0.25% trypsin for 1 hour (37 ° C., 5% CO 2) was followed by overnignt with shaking at 2 mg / ml collagenase type I (Sigma, C0130). The cell suspension thus obtained was filtered through a 100 μm pore size cell strainer (BD Falcon, 352360, USA) and centrifuged at 1,600 rpm for 10 minutes to wash the centrifuged cells three times with Hank's balanced salt solution (Hyclone SH30031.01). After that, chondrocytes were obtained. The cells obtained were 10% FBS (Fetal Bovine Serum, Hyclone, SH30396.03) and 1% penicillin-Streptomycin (Hyclone, SV30010) and 1% L-glutamine (Hyclone, SH30034.01) in DMEM (Hyclone, SH30243.01). And 0.1% Gentamycin sulfate (Lonza 17-518Z, USA) was added to incubate in a 75T flask.

1.4 Evaluation of chondrocyte activity in vitro

1) Cell Viability Measurement

Chondrocytes obtained from primary cultures were stabilized for 4 hours by dispensing cells in DMEM culture at a concentration of 1x10 ⁴ cells / well in 96well plate, and then treated with lacquer extract for 72 hours (toxicity test) or 4 hours (H Incubated for ₂ 0 ₂ ).

20 μl of 3- (4,5-methylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) reagent was incubated in a 37 ° C. incubator for up to 4 hours, then the medium and MTT reagent were removed. Μl was added and measured using an ELISA reader (VERSAMAXSL-20 Molecular Devices, Korea) at 560 nm. Three measurements yielded the mean and standard error.

2) Evaluation of chondrocyte activity-sodium sulfate incorporation

Sodium sulfate acts as a component of the biosynthesis of proteoglycans.

In general, many methods have been used to confirm the degree of biosynthesis by treating embryonic tissues that are easily absorbed into cells. However, in this experiment, chondrocytes that are still in the process of growth are separated from rats at 4 weeks of age. I did it.

1.5 Expression of Collagen and MMPs in Vitro

After culturing chondrocyte cells collected from rat cartilage, based on cytotoxicity test, after treatment with samples and H ₂ O ₂ , mRNA was extracted using Quiagen RNeasy mini kit (USA, 74106) and the gene expression level was measured at the cellular level. It was. Collagen and MMPs were measured by Real Time PCR. (Applied Biosystem, Step One Real-Time PCR System, USA)

2. Experimental Results 1

In vitro Cell Death Inhibitory Effects of Fractions Obtained from Sumac Extracts

  In the present invention, the extract of the lacquer tree was investigated for the inhibition of in vitro cell death in primary cultured chondrocytes.

 Prior to performing in vivo experiments, it was decided to first select possible concentrations through in vitro experiments. Cytotoxicity in the lacquer hot water extract fraction was not shown as shown in FIG. 3.

That is, as a result of confirming the significance of the cytotoxicity of the lacquer extract at p <0.05 value by the Multiple Range test of chondrocytes determined by MTT assay ANOVA in FIG. 1, there was no cytotoxicity.

In addition, as shown in FIG. 4, the effect of protecting or inhibiting chondrocytes from H ₂ O ₂ induced apoptosis in the lacquer hot water extract was the highest at the sample concentration of 300 μg / ml.

2.2 Assessment of chondrocyte activity-sodium sulfate incorporation

The results of the experiment showed that the influx into the cell was generally observed, but there was no significant change between the experimental treatment groups.

However, the highest cell influx was observed at the concentration of 200 ug / ml of the lacquer hot water extract, and the overall effect of the sample was found.

That is, as shown in FIG. 5, the effect of the lacquer hot water extract on the incorporation for intracellular proteoglycan synthesis of isotope labeled sodium sulfate (Na235SO4) of articular cells was confirmed to be significant at p <0.05 value by ANOVA after Multiple Range test. Can be.

2.3 In Vitro Potency Test of Fractions Obtained from Hot Water Extract of Sumac (Real Time PCR)

As a result of performing Real Time PCR in FIGS. 6 to 7, the positive factors, Type 1 Collagen, Type 2 Collagen, and Aggrecan, seem to increase the production of collagen and aggrecan at a concentration of 100 ug / ml. Was found to appear better.

In addition, the results of the measurement of MMPs, which are Negative factors in FIGS. 8 to 9, also showed a decrease in MMPs in the group treated with Sumac hot water extract samples compared to NC (Negative Control) treated with H ₂ O ₂ only. In particular, the tendency of MMP3 and MMP7 to decrease at a concentration of 100ug / ml was obvious.

3. Experiment Result 2

The present invention is to perform the in vitro efficacy evaluation on the inhibition of chondrocyte death through the fraction of the lacquer hot water extract can isolate and identify the bioactive substances effective for arthritis.

First, the appropriate dose to be used in the experiment was determined to 300 μg / ml all by MTT cytotocixity assay and treated to cells, and cytotoxicity was induced using H ₂ O ₂ .

It was found that there was no cell toxicity in the lacquer hot water extract fraction, and there was a significant killing effect after H ₂ O ₂ treatment and it was most effective at 300 μg / ml concentration.

  According to the results of the chondrocyte activity evaluation, the inflow into the cells appeared generally, and the highest cell inflow was confirmed at the concentration of 200 ug / ml of the lacquer hot water extract.

In addition, the expression of collagen and MMPs, which are indicators of osteoarthritis, was shown to be most effective at 100 ug / ml of lacquer extract.

This is slightly lower than the concentration of 300 ug / ml, which was the best in the previous cytotoxicity test and the antioxidant protective effect test.

However, this result can be expected to have a positive effect at a slightly lower concentration than in the direct cell experiment, considering the expression level at the gene level rather than the death of the cell itself.

In conclusion, the experimental results at the cellular level confirmed that the lacquer extract fractions were effective in strengthening bone mineral density through bone formation and provide a positive effect on osteoarthritis.

The invention being thus described, it will be obvious that the same way may be varied in many ways. Such modifications are intended to be within the spirit and scope of the invention as defined by the appended claims.

Claims (4)

A composition for the prevention and treatment of degenerative arthritis containing lacquer extract obtained by dipping C1-4 lower alcohol, alcohol alcohol, acetone extract or hot water extraction of lacquer tree with C1-4 lower alcohol and alcohol alcohol.
The method of claim 1,
C ₁₅ H ₁₂ O ₆ (molecular weight 288), fractionated from P. lacquer extract, Fustine (3,3 ', 4', 7-tetrahydroxyflavanone) with white crystals, C ₁₅ H ₁₀ O ₆ (molecular weight 286), yellow Fisetin (3,3 ', 4', 7-Tetrahydroxyflavone) with crystals of C ₁₅ H ₁₀ O ₅ (molecular weight 272) and dark orange crystals Sulfuretine (3 ', 4', 6'-Trihydroxyaurone), C ₁₅ H ₁₂ O ₅ (molecular weight 272) and orange crystals Butene (2 ', 3,4,4'-Tetrahydroxychalcone), C ₁₅ H ₁₂ O ₅ (molecular weight 272) Garbanzol, C ₁₅ H ₁₀ O ₇ (molecular weight 302) Quercetin, C ₁₅ H ₁₀ O ₅ (molecular weight 270) 5-deoxykaempferol, C ₆ H ₆ O ₂ (molecular weight 110) Resorcino, C ₇ H ₆ O ₄ (molecular weight 154) in _{3,4-Benzoic acid, C 7 H} 6 O 4 ( molecular weight of 154) of _{2,4-Benzoic acid, C 7 H} 6 O 5 ( molecular weight 170) of _{Gallic acid, C 9 H 8 O} 4 Single component of Caffeic acid (molecular weight 180), Adenosin with C ₉ H ₁₁ O ₄ N ₅ (molecular weight 253) or Niacin with C ₆ H ₅ O ₂ N (molecular weight 123) A composition for the prevention and treatment of degenerative arthritis containing sumac extract, comprising the vagina
The method of claim 1,
The composition may be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, oral formulations of aerosols, external preparations, suppositories, and sterile injectable solutions according to conventional preparation methods. Composition for the prevention and treatment of degenerative arthritis containing extract
Fustine (3,3 ', 4', 7-tetrahydroxyflavanone) and C ₁₅ H ₁₀ O ₆ (C ₁₅ H ₁₂ O ₆ (molecular weight 288) fractionated from the lacquer hydrothermal extract according to claim 1) Fistin (3,3 ', 4', 7-Tetrahydroxyflavone) with yellow crystals, molecular weight 286), C ₁₅ H ₁₀ O ₅ (molecular weight 272) and Sulfurintin with dark orange crystals (3) ', 4', 6'-Trihydroxyaurone), C ₁₅ H ₁₂ O ₅ (molecular weight 272) and orange crystals Butene (2 ', 3,4,4'-Tetrahydroxychalcone), C ₁₅ H ₁₂ O Garbanzol with ₅ (molecular weight 272), Quercetin with C ₁₅ H ₁₀ O ₇ (molecular weight 302), 5-deoxykaempferol with C ₁₅ H ₁₀ O ₅ (molecular weight 270), Resorcino, C ₆ H ₆ O ₂ (molecular weight 110) ₇ H ₆ O ₄ (molecular weight of 154) of _{3,4-Benzoic acid, C 7 H} 6 O 4 ( molecular weight of 154) of _{2,4-Benzoic acid, C 7 H} 6 O 5 ( molecular weight 170) of Gallic acid, C ₉ H ₈ O ₄ (molecular weight 180) of _{Caffeic acid, C 9 H 11 O} 4 N 5 of the (molecular weight 253) of _{Adenosin, C 6 H 5 O 2} N ( molecular weight 123) Niacin One for the improvement of osteoarthritis containing sumac extract, characterized in that the active ingredient of the component material obtained by mixing and allowed acceptable carrier by an ordinary health food as the active ingredient dietary supplement

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