KR20120117956A - Composition of harmful insect repellant using phytol - Google Patents

Abstract

    The present invention relates to pests, in particular mosquito repellent, of the Phytol component derived from plants. The phytol component is a diterpene, which shows not only the initial repelling force but also the continuous repelling effect. Will be displayed.

Description

Composition of harmful insect Repellant Using Phytol

The present invention relates to a pest repellent composition, and more particularly to a control composition of long-term sustained mosquitoes by mixing mosquito blood and other plant-derived terpenes using phytol components extracted from plants.

Female mosquitoes of mosquitoes need to be supplied with animal protein for maturation of eggs, and thus close to humans or animals to make vampires. Mosquitoes' blood-sucking activity is a vehicle for major diseases such as malaria, dengue hemorrhagic fever, yellow fever, encephalitis, and filariasis.

The main species of this disease-borne species is Culex tritaeniorhynchus ), Culex pipiens pallens , Culex pipiens molestus ), Chinese spotted mosquito ( Anopheles) sinensis ), etc., typically, when pathogenic viruses or bacteria are hidden in the salivary glands of mosquitoes, viruses or bacteria are mediated through saliva during blood-sucking activities.

Due to global warming and environmental changes caused by the El Nino phenomenon on the Korean Peninsula, mosquitoes are frequently appearing and the area of occurrence is increasing, densities are increasing, and people's living standards are increasing, and the outdoor population is increasing. As the exposure time to mosquitoes increases, the importance of control becomes more important. Humankind has made various efforts to control such mosquitoes, but even now, the damage caused by mosquitoes has not been reduced, and the solution is a difficult task. There are many ways to control mosquitoes, and the most commonly used method of mosquitoes invading indoors or outdoors is chemical control through insecticides and evasions. Chemical control is also carried out through various spraying at the city and county level, but in order to control mosquitoes attacking at home or in outdoor activities, the aerosol formulation or the mat electronic mosquito infused with pesticides and wicks are embedded. The bottle contains a liquid treated with pesticides, a liquid electronic mosquito flavor that volatizes the pesticides by heating the wick, and a linear mosquito flavor that burns and smokes the medium carrying the pesticide. However, the application of pesticides in an unrestricted space, such as outdoors, is ineffective for controlling mosquitoes and has a limited insecticide effect because the concentration of the insecticide applied is rapidly diluted in the air unless the insecticides of the mosquitoes exceed the threshold concentration. Therefore, the use of a repellent to prevent the access of mosquitoes in the outdoor space is more efficient.

The most widely used compound for mosquito repellent is DEET ( N , N -diethyl- m -toluamide or N , N -diethyl-3-methylbenzamide), which has been widely used around the world since the 1950s and have been used for almost half a century. It is by far the most widely used and commercially successful ingredient. However, compared to DEET's excellent repelling effect, safety of pregnant women and children with high skin permeability is uncertain, and also has been found to be easily lost by sweat or rain, causing discoloration of plastics and synthetic fibers. Therefore, many studies have been conducted to replace the DEET, and one of such activities is actively researching plant extracts as a candidate candidate for substitute substances.

There have been many researches on natural products for eco-friendly development, and citronella is known as a mosquito repellent as a plant ingredient. In addition, eucalyptus, tea tree, cedar wood extracts have been suggested as repellents of insects. Recently, clove oil mosquitoes with fennel and neem have been suggested. The avoidance effect has been disclosed.

It is an object of the present invention to provide a pest control composition of plant origin showing a high repelling effect as a repellent means for controlling pests, in particular mosquitoes. In addition, another object of the present invention is to prepare a pest repellent composition that can provide a prescription for sustaining the repelling effect with only the repellent component of the plant origin through the release control (Releasing control) by prescribing the rapid volatilization of the existing plant origin repellent component To provide.

In order to accomplish the object of the present invention as described above,

It provides a method for producing a repellent composition against pests, in particular mosquitoes using the phytol extracted from the leather wood as an active ingredient.

In addition, the composition may further comprise plant-derived terpenes, in this case, the plant-derived terpenes are preferably those having a lower molecular weight or lower boiling point than phytol, wherein the plant-derived terpenes are carbonyl acetate and / or organic You can also use Knol.

In order to avoid mosquitoes according to the present invention, phytol (Phytol (3,7,11,15-Tetramethyl-2-hexadecen-1-ol) extracted from plants) was used as a main component. The phytol component is generally obtained as a chlorophyll component of the plant, and can be derived synthetically from monotelpans (C10 compounds) by semisynthesis or by total synthesis from acetylene, acetone, levulinic acid or isoprene, etc. have.

In the present invention, in order to obtain a phytol from the plant is a perennial wood plant ( Ailanthus altissima ), a year-old cruciferous plant, Brassica , was used to confirm the phytol effect of this common plant origin. campestris Verification).

Pytol (C20H40O) is a diterpene alcohol and has a molecular weight of 296.54 (M.W.) and a boiling point of 335.48 ° C. Therefore, phytol (C20H40O) is a plant-derived terpene.

The present invention also demonstrates the effect on mosquito skin by extracting from plants, such mosquito skin from the raw material company (Sigma-Aldrich Co.) and by using a mixture of known mosquito vegetation active ingredient known from the beginning continuously mosquito avoid It reviewed the desired prescription which may represent a synergistic effect, a mosquito repellent effect of the composition of thymol (thymol) and carbonyl acetate (Bornyl acetate) known as bactericidal, insecticidal effect of the existing insect island thyme (Tymus quinquecostatus var. japonica) It was demonstrated and the mixing effect was also confirmed.

As the active ingredient of the known mosquito repellent plant, the present invention has been proposed as an active ingredient of Clove oil in the Republic of Korea Patent Registration No. 10-0756397 "The Composition for Mosquito repellent" Knoll (Eugenol (C10H12O2, 164.22 MW)) was used.

In addition, vanillin, polyvinyl ether-maleic acid copolymer (PVM / MA copolymer), polyvinyl alcohol, polyacrylic acid, and polyvinyl are used to stabilize oil components of such plant-derived terpenes or to improve skin application stability. Ingredients selected from pyrrolidone (PVP), carbopol, hydroxy propylmethyl cellulose, hydroxy ethyl cellulose, hydroxy propyl cellulose, gelatin, and alginic acid may be used as an adjuvant.

Application formulations of the plant-derived invention ingredients for mosquito repellent include aerosols, pump sprays, spray-type liquid bottles, formulations that are naturally volatilized, such as perfumes, patches that are impregnated with non-woven fabrics, and applied to skin or clothing, etc. Various forms are all possible.

An effect of the present invention is to provide a plant-derived repellent component replacing the existing DEET component by utilizing the mosquito repellent component of the plant-derived phytol component. The phytol terpene component has a characteristic of exhibiting a continuous repelling effect that can solve the problem caused by the rapid volatilization of the repellent component of the plant as well as the excellent self-repelling effect, compared to the conventional plant-derived terpene components.

In addition, because of the continuous repellency sustainability of the phytol component as described above, by using it in combination with terpenes of the plant-derived repelling effect having the initial volatilization characteristics, the plant-derived terpene that can further supplement the initial mosquito avoidance efficiency and maintain mosquitoes You can complete the prescription consisting of the active ingredient only.

1 is a process table for the separation of the active ingredient from the leather.
Figure 2 is a mosquito evasion experiment bioassay photo using the volunteer hand.
Figure 3 is a graph of the GC-EAD response of the adult white mosquito mosquito (A) and small red mosquito (B) for the leather F24 fraction.
4 is a mass spectrum of phytol from leatherwood F24 fractions.
5 is a schematic diagram of avoidance experiment olfactorymetry.
6 is a photograph of a long-term sustained bioassay scene using a cotton cloth in the respiratory test pictograph.

Hereinafter, the present invention will be described in more detail with reference to preferred embodiments. The following examples may be modified in many different forms and should not be construed as limited to the embodiments set forth below. Embodiments of the invention are provided to more fully describe the present invention to those skilled in the art.

[ Example Plant-derived Phytol  Extraction of Ingredients

Extraction of plant-derived phytol components according to the present invention was performed through the following procedure. First, the leather tree is dried and pulverized, and 50 g of leaves and branches are taken, pour 1 liter of methanol, shaken well, and left in a dark room for 3 days, and then filtered with a filter paper and a rotary vacuum concentrator. The mixture was concentrated under reduced pressure at 45 ° C. to obtain a methanol extract.

20 g of crude extract was taken in order to sequentially partition the main components of the hides according to polarity. This was dissolved in 800 ml of distilled water, poured into a 2000 ml separatory filter, and then the same amount of hexane was added to the sample and the two solvents were mixed well. After standing for about 1 hour, only the hexane layer was decompressed and concentrated in two separated layers, and 800 ml of fresh hexane was poured into the water layer, and the same procedure was repeated to obtain about 3.2 g of hexane layer. 800 ml of chloroform was poured into the remaining water layer, and the same procedure was repeated to obtain 0.42 g of chloroform. g of ethyl-acetate layer were obtained. The remaining water layer was decompressed and concentrated to obtain 14.5 g (Fig. 1).

And hexane (hexane fr.), Chloroform (chloroform fr.), Ethyl-acetate (ethyl-acetate fr.), Fractions as shown in Figure 1 for the separation of the active ingredient of each extract The bioassay was carried out, and the fractions having activity were selected, separated and purified according to the results. After dropping 1125 g of silica gel into hexane using LPPE (Low-and medium-pressured pumped elution (Merch 70-230 mesh, 6 × 60 cm)), the hexane fraction showing the activity was dropped. Loaded. The mobile phase solvent was flowed 10 ml / min in each ratio using hexane and ethyl-acetate. The spot pattern developed on the TLC plate (SILC / UV 254, 0.25 mm, Merck KGaA, Germany) by the thin layer chromatography (TLC) after taking out the flask constant Iodine (I) reaction was confirmed after checking with a UV hand lamp (UVGL-58, UV-254 / 356nm, UVP Inc., USA), and 50% sulfuric acid solution was applied to the TLC plate using a sprayer. Sprinkle and confirm the reaction using a hot plate. In this way, solvent fractions of the Chinese cabbage, which is a cruciferous annual plant, were sequentially carried out according to the polarity through the same procedure. In addition, the same effect was examined.

[ Experimental Example  One]

The bioassay of mosquito repellent was carried out in the present invention using a white line forest mosquito ( Aedes albopictus ) and red mosquito ( Culex pipiens pallens ), using the blood-sucking hieroglyphic device as shown in Figure 2 for the repellent effect experiment of these mosquitoes, The volunteer's hand was used as the vampire medium. The application of the repellent component was evenly sprayed with 2ml of each extracted and separated material by sprayer on one of the hands, and the test insects were stabilized by spinning 50 mosquito female adults 30 minutes before As shown in the figure, both hands were exposed to the graft apparatus for 1 minute to record the blood absorption.

[ Example  One]

The mosquito repelling effect on the white line forest mosquito ( Aedes albopictus ) by the method of Experimental Example 1 was examined. As a result of the repellency test using the blood-sucking device, the repellency of the crude MeOH extract was 72.1%, and the four fractions (hexane fraction (fr.) Chloroform fr., Ethyl-acetate fr., Water fr.) The avoidance rate of 94.2, 50.0, 49.5, 53.9% was shown to be the highest activity in the hexane layer. Therefore, the hexane layer having high repellent activity was separated and purified into 5 fractions from F1 to F5 by low-and medium-pressured pumped elution (LPPE) according to the ratio of hexane and ethyl-acetate. As a result of evaluating the repellent force of adult forest mosquitoes for these fractions, it showed the highest repellent activity in F2 with 46.7, 88.9, 51.1, 54.5, and 49.1% respectively. This was again separated into five fractions (F21-25) in the same way as above, and the bioassay of them showed the highest activity by showing an evasion rate of 86.1% for the fourth fraction (F24) (Table One).

Effect of Mosquitoes on the Fractions of A. altissima on the White Mosquito Mosquito Fraction Yield (%) Repellency (Mean ± SD,%) Methanol 10.8 72.1 ± 6.3 Hexane 16.2 94.2 ± 1.7 Chloroform 2.1 50.0 ± 16.7 Ethyl Acetate 9.4 49.5 ± 6.4 Water 72.2 53.9 ± 2.4 F1 16.3 46.7 ± 17.6 F2 5.9 88.9 ± 2.8 F3 11.2 51.1 ± 1.9 F4 12.5 54.5 ± 7.9 F5 2.1 49.1 ± 1.5 F21 0.74 47.3 ± 6.3 F22 28.6 55.4 ± 20.1 F23 43.1 38.3 ± 16.8 F24 4.1 86.1 ± 3.7 F25 2.9 63.4 ± 19.2

Red house mosquitoes ( Culex) by the method of Experimental Example 1 pipiens The mosquito repellent effects on pallens ) were examined in the same way. As a result of the blood repellency test using blood-sucking device, the repellent activity of MeOH crude extract was 71.3% and 88.9% in the hexane layer. In addition, the repellent activity of 87.0 and 95.2% for F2 and F24, respectively, showed a similar or higher sensitivity to white line mosquitoes for the extract (Table 2).

Effect of Mosquitoes on the Fractions of A. altissima on Red Mosquitoes Fraction Yield (%) Repellency (Mean ± SD,%) Methanol 10.8 71.3 ± 8.8 Hexane 16.2 88.9 ± 9.6 F2 5.9 87.0 ± 11.6 F24 4.1 95.2 ± 8.2

[Experimental Example 2]

The GC / EAD system uses a 1: 1 1: 1 gas chromatography with FID detector (Gas chromatography (GC, Agilent Technologies 6890N)) and EAG (electroantennograph, Syntec stimulus Controller, CS-05, Hilversum, The Netherlands). It is a connected device that connects a computer and an integrating system, and simultaneously displays electrical waveforms when GC analysis and the smell of the smell organs in the antenna of an insect respond to the smell. For the connection of the electrodes, the antennas of the red and white line mosquitoes were cut from the head and attached to the electrode hobo. The antenna detects the volatiles coming out of the GC, and at the same time, the antenna also reacts to the antenna of the insect attached to the hobo. An electric gel (electric gel, Spectra 360, Parker Laboratories, Inc., NJ, USA) was applied between the tactile and electrodes to prevent the blood lymph from drying out and to allow current to flow.

 The principle of GC / EAD is to insert the substance to be analyzed in the injector part, and GC analyzes the substance while passing through the column, and at the same time, the odor source, which is part of the chemical splitting out of the splitter, It is connected to the antenna and draws a waveform through the EAG. At this time, GC and EAG react at the same time, confirming the analysis of the substance and the reaction to the substance.

The tactile conduction reaction through GC-EAD was carried out after the establishment of the mechanical system through pre-test to reduce the error of the mechanical reaction before each experiment. Colum used in GC was HP-5 (ID 0.25mm x 30m length), carrier gas was used as N2 gas, oven temperature was 80 ~ 250 ℃ (10 ℃ / min). . In addition, the inlet temperature was 300 ° C., and the detector temperature was detected under 300 ° C. conditions.

[Example 2]

GC-EAD was used to search for F24 actives. That is, the F24 active material showed a high repellent effect, the experiment was carried out according to the procedure of Experimental Example 2 to confirm and search the antenna response of the mosquitoes.

GC-EAD was used to confirm the antenna responses of adult white mosquitoes and adult cicadas. F24, the highest activity, was diluted in hexane in an appropriate amount and 1 µl was injected through GC, and the antenna response of each insect was observed. As a result, the reaction of the white line mosquito was able to confirm through the wavelength of the charge flow as shown in FIG. The white line mosquito showed an antenna response to the peak at retention time (RT) 645s in the GC analysis of F24. In addition, it was confirmed that the same material acts on the reaction between the forest mosquito and the collecting mosquito by showing the antenna response to the peak of the same retention time.

[Experimental Example 3]

For peaks in the mosquito's antenna response to the material in the GC / EAD assay, the active material was identified using GC / MS. The collum used for the analysis of Gas chromatography-mass spectrometry (GC / MS, Agilent Technologies 7890A / 5975C) is HP-5 (ID 0.25 mm x 30 m length), and the carrier gas is helium (He) gas was used, the oven temperature was set to 80 ~ 250 ℃ (10 ℃ / min). In addition, the inlet temperature was 300 ° C., and the detector temperature was detected under 300 ° C. conditions.

[Example 3]

In GC-EAD, the spectrum, molecular weight and pattern of the active substance were determined using GC / MS to identify a substance with a retention time of 645 s, which showed the same antenna response between the white-row mosquito and the red-cold mosquito. It confirmed on condition of 3.

As a result, the molecular weight of the active material extracted from the perennial plant leather and the annual cabbage was 296, and it was confirmed that the same material as the phytol C ₂₀ H ₄₀ O by comparing the molecular weight and spectrum in conjunction with the database ( 4).

[Experimental Example 4]

In order to test the avoidance effect using the olfactory system, an olfactory meter (ID: 2.8 cm; arm length: 26.3 cm; stem length: 6.3 cm; angle between arms: 180 °) as shown in FIG. 5 was used. The olfactory meter uses a vacuum pump (Thomas medipump ^? ) To flow air at a constant flow rate (100 ml / min) from both arms to the outside of the olfactory meter so that insects are not affected by the smell at the beginning of inoculation. Treatment of the material on the side cancers caused mosquitoes to fly, so it was designed to choose one of the two arms. The air flowing through each arm was filtered through activated charcoal, molecular sieve, and silica gel blue to allow fresh air to flow. At the distal end of one arm was placed a filter paper, or a seedling (leather) port, that treated a certain amount of sample using a micro applicator. After inoculation of the adult mosquitoes at the distal end of the stem, it was recorded which of the two cancers was selected within 5 minutes. After the 10 repetitions were performed in consideration of the influence of the direction, 10 repetitions were experimented by rotating 180 °. The olfactory meter used once was washed with ethanol and distilled water, and then sufficiently dried in an oven at 100 ° C. for use in the next experiment.

Experimental conditions were maintained at 25 ± 2 ℃, relative humidity 50 ~ 60%, photoperiod 16L: 8D. White streaks and red mosquitoes were used in the experiment.

Example 4

Phytol, an active substance identified by GC / EAD and GC / MS, was purchased from Sigma-Aldrich Co. (97%, USA). Was performed.

Phytol was diluted to three concentrations of 7.5, 0.75, and 0.075% and sprayed on one hand to test the repellency of two mosquitoes. As a result, both the white line moss and the red mosquito showed 100% avoidance at 7.5% concentration, and the red mosquito showed high susceptibility above 95% even at the concentration of 0.75%. In addition, even at the lowest concentration of 0.075%, two mosquitoes showed an evasion rate of 70% or more (Table 3).

Avoidance Effect of Pitol in Concentrations of White Line Forest Mosquito and Red Mosquito Insect Conc. (%) Repellency (Mean ± SD,%) Ae. albopictus 7.5 100.0 ± 0.0 0.75 72.0 ± 12.5 0.075 74.9 ± 23.9 Cx. pipiens pallens 7.5 100.0 ± 0.0 0.75 94.4 ± 9.6 0.075 70.3 ± 10.2

[ Example  5]

Isle thyme ( Tymus quinquecostatus var. Japonica ) is known to kill insects and microorganisms of existing insects, but the repellent effect on insects is unknown. In particular, it was confirmed in the present invention that there is a repellent effect on mosquitoes, the main component was confirmed using GC-MS, and the repellent effect of the mosquitoes for each component was confirmed.

A white algae mosquito ( Ae. Albopictus ) was used as a test insect, and as a result of examining the repellent effect on island thyme by the method of Experimental Example 1 (Table 4), thymol (Chymol (C ₂₇₎ constituting the main component of the island thyme H ₃₀ O ₅ S, 466.59 MW)) was found to show a very high content and to show the repellent action of mosquitoes at a high content, but it is a bonyl acetate (C ₁₂ H ₂₀ O ₂ , 196.29 MW)) was very high and excellent.

Repellent Effect of Major Components of Island Thyme ( Tymus quinquecostatus var. Japonica ) against White Line Mosquito Peak
Number Major components Dose (μl) Repellency (Mean ± SD,%) One a-Pinene 0.578
0.289 49.0 ± 2.9
43.6 ± 2.8 2 Camphene 0.270
0.135 54.6 ± 1.2
52.0 ± 4.4 3 Myrcene 0.996
0.498 55.5 ± 2.1
51.7 ± 3.5 4 a-Terpinece 1.070
0.535 52.9 ± 5.9
51.3 ± 3.4 5 Limonene 1.070
0.535 46.1 ± 1.4
- 6 γ-Terpinene 7.100
3.550 52.4 ± 4.8
47.4 ± 2.4 7 p-Cymene 1.776
0.888 51.9 ± 4.0
47.0 ± 5.7 8 Bornyl acetate 0.868
0.434 100. ± 0.0
91.3 ± 5.9 9 Caryophyl lene 0.868
0.434 53.9 ± 1.0
50.3 ± 2.6 10 Borneol 1.684
0.842 95.2 ± 8.2
59.9 ± 3.8 11 Thymol 7.360
1.609 100. ± 0.0
81.7 ± 1.7 12 Carvacrol 0.268
0.134 52.4 ± 2.3
-

Therefore, in order to find out the mosquito repellency over time for the phytol found to be the main mosquito repellent component derived from plants in the present invention, eugenol presented in carbonyl acetate (97%, Sigma-Aldrich Co.) and Korean Patent Registration No. 10-0756397 (98%, Sigma-Aldrich Co.) components were compared or included and examined.

[ Experimental Example  5]

The repellency test over time was applied up to 10 minutes, 30 minutes, 1 hour, 2 hours, 4 hours after each sample treatment, wherein the experimental method using the pictograph shown in Example 1, Figure 6 Similarly, using a rubber glove cut into the back of the hand with a diameter of 5.5 cm, the evasion effect was evaluated by placing a shirt piece treated with each repellent component on the rubber gloves. At this time, the cotton cloth (diameter 5.5cm) is a sample in which 5% concentration is previously dissolved in ethanol solvent and uniformly treated 1 ml of repellent component, and then volatilized the main component of repellent at room temperature without direct sunlight for each hour. It is the same size as a hole (5.5 cm in diameter) made in cotton. About 50 animals were applied to each of the white line mosquitoes and red house mosquitoes by experiment. At this time, the number of mosquitoes sitting on the cotton cloth (landing No.) and the number of vampires (bites No.) were both counted as behavior (behavior). At this time, the landing (landing) on the cotton cloth was considered to be a valid landing when staying in the landing state for more than 3 seconds.

[Examples 6 to 10]

According to the composition shown in Table 5, phytol and carbonyl acetate, eugenol, respectively, and a mixed repellent composition was obtained to obtain a repellency efficiency results over time.

division Terpenes Dose
(5%) Evasion rate according to (Houre after Treatment) 10 min 30min 1h 2h 4h 6h Example
6 Phytol
(P) 1 ml 97.3 ± 2.3 98.2 ± 2.0 96.7 ± 1.2 94.0 ± 2.0 91.3 ± 1.2 86.0 ± 5.3 Example
7 Bornyl
acetate
(b) 1 ml 100. ± 0.0 98.0 ± 2.0 87.3 ± 6.1 78.7 ± 7.0 69.3 ± 8.3 62.7 ± 3.1 Example
8 Eugenol
(E) 1 ml 100. ± 0.0 98.7 ± 2.3 94.7 ± 3.1 85.3 ± 5.8 74.7 ± 9.5 68.7 ± 2.3 Example
9 P + B 0.5ml + 0.5ml 100. ± 0.0 99.3 ± 1.2 99.3 ± 1.2 97.3 ± 2.3 96.0 ± 2.0 92.7 ± 3.1 Example
10 P + E 0.5ml + 0.5ml 100. ± 0.0 100. ± 0.0 98.7 ± 2.3 99.3 ± 1.2 96.7 ± 1.2 94.7 ± 4.2

* Evasion rate = (number of applied mosquitoes-vampire or landing) / number of applied mosquitoes * 100

Experimental results showed that terpenes such as carbonyl acetate and eugenol had excellent initial repelling power, but the repelling efficiency decreased rapidly with time, and phytol continued to repellent, especially phytol and initial repelling power with excellent repelling persistence. When terpenes exhibiting a mixed treatment, it was confirmed that both the initial repellency and the repellency persistence.

This effect is similar to Experiment 5 with the same prescription of phytol (5%, 1ml), eugenol (5%, 1ml), phytol (5%, 0.5ml) + eugenol (5%, 0.5ml). Repetitive experiments were carried out on the red mosquito and confirmed with almost similar results.

It is assumed that the phytol component has a high boiling point (335.48 ℃) tendency with a high molecular weight (296.54), and in the present invention, the phyto component of the plant origin shows a continuous mosquito repelling force and at the same time the initial repelling force (by rapid volatilization) It was confirmed that mosquito repellent effect with early and sustained efficacy was shown only with plant origin ingredients through high general terpenes and mixed prescription. The repellent effect of phytol in the present invention is limited only to mosquitoes, but can be applied to other general pests, and since the action point is different from the insecticidal effect and the bactericidal effect of microorganisms, continuous examination can be made.

In addition, in the present invention, carbonyl and eugenol mixed with phytol are generally presented as examples of plant-derived terpenes having high initial volatility, and general plant essential oils have various terpenes having such a rapid initial volatilization. It is composed of such plant essential oils. Therefore, the plant-derived terpenes having high initial volatility in the present invention are not limited to the practice of the present invention.

Claims (8)

A pest repellent composition comprising phytol as an active ingredient. The pest repellent composition of claim 1, wherein the phytol is extracted from a plant. 3. The pest repellent composition according to claim 2, wherein the plant is a leather tree. The pest repellent composition of claim 1, wherein the pest is a mosquito. According to claim 1, wherein the composition is vanillin, polyvinyl ether-maleic acid copolymer (PVM / MA copolymer), polyvinyl alcohol, polyacrylic acid, polyvinylpyrrolidone (PVP), carbopol (carbopol), hydroxy propyl A pest repellent composition comprising at least one selected from methyl cellulose, hydroxy ethyl cellulose, hydroxy propyl cellulose, gelatin, and alginic acid. The pest repellent composition according to claim 1, wherein the composition further comprises plant-derived terpenes. 7. The pest repellent composition according to claim 6, wherein the plant-derived terpenes have a lower molecular weight or lower boiling point than phytol. 8. The pest repellent composition according to any one of claims 6 or 7, wherein the plant-derived terpenes are carbonyl acetate and / or eugenol.

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