KR20120087408A - Composition for antioxidation effect comprising sparassis crispa extract - Google Patents

Abstract

PURPOSE: A cosmetic composition containing fermented Sparassis crispa extract is provided to enhance oxidation and anti-wrinkling effects with safety and stability. CONSTITUTION: A fermented Sparassis crispa extract is prepared by fermenting and extracting Sparassis crispa fruit body using fermentation liquid. The fermentation liquid is Lactobacillus, yeast, photosynthetic bacteria, and Actinomycetes. A cosmetic composition contains for skin whitening, anti-wrinkling, and UV protection contains 0.0001-20.0 wt% of the extract. The cosmetic composition is manufactured in the form of a skin toner, astringent, lotion, nutrition cream, massage cream, essence, gel, pack, powder, ointment, patch, suspension, emulsion, or spray.

Description

Flower mushroom fermented extract having antioxidant activity and cosmetic composition containing same {COMPOSITION FOR ANTIOXIDATION EFFECT COMPRISING SPARASSIS CRISPA EXTRACT}

The present invention relates to a cosmetic composition having an antioxidant activity, and more particularly, to provide a cosmetic composition using the antioxidant activity of the flower mushroom fermented extract, which is safe for the skin and has an excellent antioxidant and anti-aging effect with excellent stability of the formulation. It relates to the cosmetic composition of the.

Today, there are at least 10,000 species of mushrooms, of which about 600 are edible. The book, Chinese Medicinal Fungi, describes 117 mushrooms used for medicinal purposes. In this book, not only the mushrooms that have been used for medicinal purposes such as Cordyceps sinensis and Ganoderma lucidum, but also the efficacy of edible mushrooms such as thirsty, spinning top and shimeji are recorded. This is because mushrooms contain rich nutrition such as proteins and minerals, and unique fragrances, and thus, mushrooms are highly likely to be used as favorite foods and health foods.

In particular, the blossom mushrooms were collected in the wild, and in 1997, Fukushima, a teacher at Saitama High School in Japan, developed the sawdust bottle cultivation method for the first time, and began to study cultivation in earnest. Since 1999, research on mass cultivation has paid off, and in the 2000s, full-scale commercial production and product development have been actively conducted. These blossom mushrooms are attracting attention and attention worldwide because they are known to contain 43.6 g of beta-glucan per 100 g of fruiting body and have been recognized for their functionality. This is a medicinal mushroom, which contains beta-glucan more than three times than generalized mushrooms, and is one of rare mushrooms handled by only a few companies in Korea.

Although the patented technology related to zinnia mushrooms is introduced in KR1020050082982, KR1020080091706, KR20050104961, KR1020020024560, KR1020020058355, and KR1020070035261, most of them are patented technologies related to cultivating zinnia mushroom, and similarly to the present invention, the invention using physiological activity of zinnia mushroom Nothing was reported.

In general, the main theories related to aging in the human body include active oxygen theory that is caused by cellular damage caused by free radicals, biological clock theory based on bioinformation in DNA, and restriction of DNA replication and cell death by telomeres. Telomerism is most convincingly discussed in academia, but it is not clear yet. To this end, many researchers have tried to identify the root causes of aging from various perspectives based on various experimental results, and various studies on aging have the purpose of ultimately extending life and healing diseases. The most persuasive argument among these various aging-related theories is that free radical theory that cell damage by free radicals is one of the causes of aging is most persuasive to researchers.

In association with free radicals, aging of the skin can be observed by physical changes such as wrinkles, which is believed to be due to damage of cells in the skin by free radicals. That is, the skin is exposed to air pollutants such as NOx or SOx, or ultraviolet rays, and the oxidative stress in the body is increased, and if the skin is continuously exposed to the oxidative stress environment, the radicals in the body are increased. Various biochemical reactions can be caused to destroy skin tissue and cause skin wrinkles. In other words, when the skin is exposed to oxidative stress such as ultraviolet light, it promotes the expression of collagenase and elastase, enzymes that break down collagen and elastin. The collagen and elastin that make up the tissues are broken down, causing certain parts of the skin tissue to settle down, or skin wrinkles. Therefore, many researchers are focusing on researches on antioxidants that reduce oxidative stress and on the enhancement of collagen or elastin biosynthesis of skin tissue, and various substances have been studied and reported.

The major radical components associated with skin aging are mostly reactive oxygen species (ROS), mainly super oxide radicals, hydrogen peroxide (H ₂ O ₂ ), hydroxy radicals, or single There is a single state of oxygen (singlet oxygen). These reactive oxygen species are considered to be the main cause of cancer, tissue damage, and aging. For example, the peroxidation of the membrane lipids, which is a constituent of cells, may be related to the peroxidation of skin cell membranes and the modification of dermal constituent proteins. It is known.

Skin antioxidants known to date have anticipated effects such as improved skin moisturizing activity, antioxidant activity against various free radical species, promotion of skin fibroblast proliferation, inhibition of expression of collagenase and elastase, and promotion of collagen and elastin biosynthesis. It is being developed or studied with. As described above, since free radical type oxygen or reactive oxygen species are highly related to skin aging, conventionally, cosmetics may include superoxide dismutase, vitamin E and derivatives thereof, vitamin C and derivatives thereof, or Although many efforts have been made to prevent skin aging by blending flavonoids, etc., it is difficult to expect substantial effects due to poor safety and stability when blending the above ingredients in cosmetics. In addition, although BHT (buylated hydroxytoluene) and BHA (buylated hydroxyannisole), which are synthetic antioxidants, have strong antioxidant activity, research reports that ingesting more than 50 mg / kg / day of BHT cause cancer. Consumers' rejection is pointed out as the biggest disadvantage.

Therefore, many studies have been conducted to find a substance having antioxidant activity in natural materials such as foods, but most of them have an antioxidant ability that does not meet expectations. The necessity for the development of the clinic continues to be urgently required.

On the other hand, the process of decomposing organic matter by using enzymes of microorganisms is called fermentation.The fermentation process is similar to the decay process, but it is called fermentation when the degradation products are useful in our lives. The distinction between corruption is the difference between general fermentation and corruption. Therefore, when fermentation of natural products using microorganisms, the toxicity of natural products is reduced, low molecular weight is easy to digest, or transdermal absorption when applied to the skin, and the bioavailability is improved is a characteristic of the general fermentation technology. In addition, natural products may exhibit their own physiological activity, but fermentation may further enhance their effects or create harmful components through the metabolism of microorganisms, resulting in new physiological activity that did not exist in natural products.

In the present invention, the antioxidant activity and the efficacy and effect of the cosmetic composition containing the fermented extract of the mushroom mushroom fermentation process for the blossom mushroom was experimentally completed to complete the present invention.

KR 20050082982 10 KR 20080091706 10 KR 20050104961 10 KR 20020024560 10 KR 20020058355 10 KR 20070035261 10 KR 20090133010 10

O. K. Chun, D. O. Kim, C. Y. Lee, "Superoxide radical scavenging activity of the major polyphenols in fresh plums", J. Agric. Food Chem. 2003, 51, pp. 8067 ~ 8072 Basu S., Hazra B. "Evaluation of nitic oxide scavenging activity, in vitro and ex vivo, of selected medicinal plants traditionally used in inflammatory diseases" Phytother. Res. 2006, 20, pp. 869 ~ 900 Hazra B., Biswas S., Mandal N. "Antioxidant and free radical scavenging activity of Spondias pinnata" BMC Complementary and Alternative Medicine 2008, 8 (63), doi: 10.1186 / 1472-6882-8-63

The present invention is to provide a new material that can supplement and improve the safety and stability of the antioxidant components commonly used in the existing cosmetic industry, more specifically, the antioxidant activity of the edible mushroom used for food fermentation Increase through the process to provide a cosmetic composition that is safe for human use and excellent in stability.

In order to solve the above problem

The present invention provides a flower mushroom fermentation extract extracted by fermenting the flower mushroom fruiting body with a fermentation broth as a means for solving the problem.

In order to more effectively solve the above problems, the present invention is a fermentation broth of the flower mushroom, characterized in that the fermentation broth (Lactobacillus), yeast (Yeast), photosynthetic bacteria (Photosynthetic bacteria), actinomycetes (Actinomycetes) Provide as a means for solving the problem.

In another aspect, the present invention is to solve the above problems through a cosmetic composition containing a mushroom mushroom fermentation extract,

The cosmetic composition is characterized in that the content of 0.0001 ~ 20.0000% by weight of the mushroom mushroom fermentation extract based on the total weight of the cosmetic composition.

Blossom mushroom fermented extract having antioxidant activity and cosmetic composition containing the same according to the present invention is safe for the skin and has an excellent anti-aging and antioxidant effect and also excellent in the sustainability of the effect.

1 is the DPPH scavenging activity of the flower mushroom fermented extract.
2 is the superoxide radical scavenging activity of the flower mushroom fermentation extract.
3 is the nitric oxide radical scavenging activity of the cultivated mushroom fermented extract.
4 is the hydroxy radical scavenging activity of the extract of the fermented mushroom mushroom.
5 is an effect of improving wrinkles according to the use of blossom mushroom fermentation extract-containing cosmetic composition.

In order to solve the above technical problems, the present invention was fermented to a mushroom mushroom using lactic acid bacteria, yeast, or photosynthetic bacteria, the cosmetic composition having an antioxidant activity, characterized in that the extract of the mushroom mushroom fermentation extract as an active ingredient The main content is to provide.

Hereinafter, for example, a detailed description of the present invention, the mushroom mushroom fermentation extract having an antioxidant activity and the cosmetic composition containing the same. However, the following Examples and Experimental Examples are only presented to more easily understand the present invention, and the contents of the present invention are not limited or limited thereto.

In order to prepare the blossom mushroom fermentation extract, the mushroom mushroom fruiting body is fermented at room temperature. The fermentation broth used at this time is composed of Lactobacillus, yeast (Yeast), photosynthetic bacteria, Actinomycetes and the like, preferably the fermentation broth is Lactobacillus plantarum, Lactobacillus casei, Streptoccus lactis, Saccharomyces cerevisiae , Candida utilis, Rhodopseudomonas palustrus, Rhodobacter spaeroides, Streptomyces albus, Streptomyces griseus, Aspergillus oryzae, Mucor hiemalis and the like. The population of fermentation microorganisms constituting the fermentation broth comprises at least 1.0 × 10 ⁴ cfu / mL for each group.

The flower mushroom fruiting body used in the present invention refers to the fruiting body grown by the method used for the cultivation of common mushrooms, such as single-cultivation or sawdust cultivation, from the flower mushroom hyphae. The fermented cauliflower mushroom fermented in this way is filtered and then concentrated completely with a rotary evaporator to give a brown syrup. Aqueous solution of ethanol is added thereto, and concentrated and sterilized to obtain a fermented extract of a mushroom of brown syrup appearance.

Example 1 Preparation of Blossom Mushroom Fermented Extract

4 kg of fruit mushroom fruiting bodies contain yeast 4.1 × 10 ⁴ cfu / mL, lactic acid bacteria 7.0 × 10 ⁶ cfu / mL and photosynthetic bacteria 1.2 × 10 ⁶ cfu / mL It was fermented in 18 L and fermented at room temperature for 3 days. The fermented cauliflower mushroom fermented in this way was filtered and then completely concentrated by a rotary evaporator to obtain about 500 g of a brown syrup having a sweet aroma. 70% ethanol aqueous solution was added thereto, followed by complete concentration with a rotary evaporator, which was sterilized with an autoclave to obtain 430 g of a mushroom extract of fermented mushroom of brown syrup.

<Example 2. Flower Mushroom Fermented Extract-Containing Cosmetic Composition>

A cosmetic composition containing the flower mushroom fermentation extract obtained in Example 1 was prepared as follows.

Formulation Example 1 Solubilized Formulation Raw material name Content (% by weight) Remarks Formulation Example 1 Comparative Formulation Example 1 Butylene Glycol 7.00 7.00 glycerin 4.00 4.00 Olive Oil Pages-7 Esters 2.00 2.00 Panthenol 1.00 1.00 Cetearyl Olate 0.50 0.50 Sorbitan olivate 0.50 0.50 Dimethicone 0.50 0.50 Sodium carbomer 0.30 0.30 Sodium Hyaluronic Acid 0.03 0.03 Allantoin 0.10 0.10 Other additives 3.00 3.00 Plant extract 3.00 3.00 Blossom Mushroom Fermented Extract 1.00 - Preservative Suitable amount Suitable amount Combination spices Suitable amount Suitable amount Purified water Balance Balance

In Formulation Example 1 and Comparative Formulation Example 1 described above, each component is precisely weighed in a balance and then dissolved in warming at 75 ° C., followed by uniform mixing. When all components are completely dissolved and mixed uniformly, it is cooled to room temperature to complete Formulation Example 1 and Comparative Formulation Example 1.

 Formulation Example 2 Emulsion Formulation Raw material name Content (% by weight) Remarks Formulation Example 2 Comparative Formulation Example 2 Butylene Glycol 5.00 5.00 glycerin 4.00 4.00 Macadamia Seed Oil 4.00 4.00 Dimethicone 3.00 3.00 Cetearyl Olate 1.50 1.50 Sorbitan olivate 1.50 1.50 Panthenol 1.00 1.00 Cyclopentasiloxane 0.50 0.50 Cyclohexasiloxane 0.50 0.50 Cetearyl alcohol 0.50 0.50 Tocopheryl acetate 0.40 0.40 Cetyl palmitate 0.40 0.40 Sorbitan palmitate 0.30 0.30 Sorbitan olivate 0.30 0.30 Sodium carbomer 0.20 0.20 Other additives 3.00 3.00 Plant extract 3.00 3.00 Blossom Mushroom Fermented Extract 1.00 - Preservative Suitable amount Suitable amount Combination spices Suitable amount Suitable amount Purified water Balance Balance

The components of Formulation Example 2 were divided into water-soluble components and fat-soluble components and weighed, and then dissolved by heating each to 75 ° C. After dissolving the two components completely, adding a mixture of fat-soluble components to the water-soluble component, emulsification at 3,000 rpm, and when the emulsion particles are homogenized, cooled to room temperature to complete Formulation Example 2 and Comparative Formulation Example 2.

The components of Formulation Example 1 and Formulation Example 2 above may be added in consideration of the stability and palatability of the formulation, or may be excluded from the components, one of the cosmetic compositions presented to help the understanding of the present invention It is intended as an example. The cosmetic composition shown in Example 2 of the present invention is a blended application of the usual ingredients to the general cosmetics as needed, the content of the oily component of the cosmetic composition containing the mushroom mushroom fermentation extract according to the present invention reinforces the emulsifying capacity And it is selected in consideration of economics and the like, as the oil which can be mainly used as an oil phase component may be used at least one selected from vegetable oil, mineral oil, silicone oil and synthetic oil. In addition, 0.1 to 5% by weight of a surfactant, a higher alcohol, etc. may be added to reinforce the emulsifying ability. As such surfactants, conventional surfactants such as nonionic surfactants, phospholipids, and the like may be used. As the higher alcohols, alcohols having 12 to 20 carbon atoms may be used alone or in combination of one or more thereof. In addition, in the cosmetic composition, the aqueous phase component may further add 0.001 to 5% by weight of one or more thickeners such as carbomer, xanthan gum, bentonite and the like to adjust the viscosity or hardness of the aqueous phase. In addition, the composition of the present invention may further be added with physiologically active ingredients such as arbutin, adenosine and vitamins, as well as ingredients used in conventional cosmetics such as sunscreens, antioxidants, preservatives, fragrances, colorants and pH adjusters.

In addition, Comparative Example 1 and Comparative Example 2 of Formulation Example 1 and Formulation Example 2 were prepared to compare the physical / chemical effects that the mushroom mushroom fermentation extract can have on the formulation.

The cosmetic composition of the present invention is a product that is absorbed through the skin, softening cream (skin toner), astringent makeup, nutrition cream (lotion), nutrition cream, massage cream, essence, gel, pack, powder, skin ointment, patch It may be prepared in the form of conventional cosmetics such as, suspensions, emulsions, sprays, or cosmetic liquids, and may include various conventional carriers and additives suitable for each of these formulations and well known in the art.

Experimental Example 1. Antioxidant Activity of Fermented Extracts of Blossom Mushroom

Using the mushroom mushroom fermentation extract of Example 1, the following antioxidant activity experiment was carried out.

1. Measurement of DPPH Antioxidant Capacity

In order to determine the antioxidant capacity of the flower mushroom fermentation extract of Example 1 according to the present invention was carried out the following experiment using the DPPH method. The DPPH (1,1-diphenyl-2-picrylhydrazyl) method is a general test method that can directly confirm the radical removal ability of a sample by measuring absorbance as radicals colored according to the oxidizing / reducing conditions. The flower mushroom fermentation extract prepared in Example 1 was dissolved in a suitable solvent at different concentrations (10, 100, 500, 1,000 ppm), and then 100 μl of the solution was mixed with 900 μl of 100 μM DPPH solution. After careful mixing, the mixture was left at room temperature for 10 minutes and absorbance at 540 nm was measured. Distilled water or a solvent was used as a blank for the blank test, and a sample consisting of only DPPH and distilled water or a solvent was used as a comparative group. Free radical scavenging activity was determined by calculating the following equation from the measured absorbance. The results are shown in FIG.

(1)

% Free radical scavenging activity = [1- (absorbance of sample / absorbance of comparative group)] × 100

As shown in Figure 1, the blossom mushroom fermented extract according to the present invention significantly increased the antioxidant activity by DPPH depending on the concentration. In addition, the IC ₅₀ of the DPPH free radical scavenging activity of the fermented extract of the mushroom mushroom was found to be 71 ppm, so that the DPPH free radical scavenging activity of the fermented extract of the mushroom mushroom was very high considering that the mixture of the mixture of various compounds It can be seen that excellent.

2. Superoxide Radical Scavenging Activity

The superoxide radical scavenging activity of the flower mushroom fermentation extract of Example 1 was described in Chun et al . (OK Chun, DO Kim, CY Lee, "Superoxide radical scavenging activity of the major polyphenols in fresh plums", J. Agric. Food Chem . 2003, 51 , pp. 8067-8072). Samples were prepared for each concentration of the mushroom mushroom fermentation extract and the superoxide radical scavenging activity was measured according to the method of Chun et al ., And the results are shown in FIG. 2.

As shown in FIG. 2, the superoxide radical scavenging activity of the flower mushroom fermentation extract was significantly increased in proportion to the concentration, and the IC ₅₀ was about 550 ppm.

3. Nitric oxide radical scavenging activity

In order to confirm the nitric oxide radical scavenging activity of the flower mushroom fermentation extract, the following experiment was performed.

The fermented extract of the zinnia mushroom of Example 1 was prepared by concentration, followed by Basu S. and Hazra B. (Basu S., Hazra B. "Evaluation of nitic oxide scavenging activity, in vitro and ex vivo, of selected medicinal plants traditionally used in inflammatory diseases " Phytother. Res . 2006, 20 , pp. 869-900). The results are shown in Fig.

As shown in FIG. 3, it was confirmed that the nitric oxide radical scavenging activity of the flower mushroom fermentation extract increased in proportion to the concentration, and the IC ₅₀ was about 350 ppm.

4. Hydroxy radical scavenging activity

The following experiment was performed to confirm the hydroxy radical scavenging activity of the flower mushroom fermentation extract of Example 1.

The mushroom mushroom fermentation extract of Example 1 was prepared for each concentration, followed by Hazra et al . (Hazra B., Biswas S., Mandal N. "Antioxidant and free radical scavenging activity of Spondias pinnata" B MC Complementary and Alternative Medicine 2008, 8 (63), doi: 10.1186 / 1472-6882-8-63) Hydroxy radical scavenging activity was measured according to the method, and the results are shown in FIG. 4.

As shown in Figure 4 it was confirmed that the hydroxy radical scavenging activity of the flower mushroom fermentation extract increased in proportion to the concentration, the IC ₅₀ was about 2,500 ppm.

The conclusions from the above antioxidant test results indicate that the fermented mushroom extract is a natural product and a mixture of various compounds, and about 10 times higher than the antioxidant activity of the blossom mushroom extract reported so far. In the sense that the mushroom mushroom fermented extract has a very good antioxidant activity and can be said to have a high industrial applicability.

Experimental Example 2 Skin Wrinkle Improvement Effect of the Flowering Mushroom Fermented Extract Composition

Using Formulation Example 1 and Formulation Example 2 of Example 2, the skin wrinkle improvement effect was measured as follows.

By using Formulation Example 1 and Formulation Example 2 of Example 2, five women in their 30s and 40s were discontinued, and the cosmetic compositions of Formulation Examples 1 and 2 were twice a day. It is to be used in the morning and before bedtime. Confirmation of the wrinkle-improving effect was evaluated by comparison before and after using the above formulation example 4 weeks after use. More specifically, the test subjects were prepared before and after using replicas using silicone adhesives (Provil, Germany), and the produced replicas were image-pro plus (Adept Electronic Solution Pty Ltd., a computer image analysis program). USA). The results are shown in Fig.

As shown in Figure 5, when using Formulation Example 1 and Formulation Example 2 was confirmed that both the depth and height of the wrinkles reduced and smoothed, the maximum reduction rate of the wrinkles was about 30%, the average reduction rate was about 16% It became. From the above experimental results it was confirmed that the cosmetic composition containing the mushroom mushroom fermentation extract has a very excellent effect in the improvement and prevention of wrinkles.

Experimental Example 3. Safety Experiment of Cosmetic Composition Containing Blossom Mushroom Fermentation>

In order to confirm the safety of the cosmetic composition of Formulation Example 1 and Formulation Example 2 of Example 2 according to the present invention was carried out a patch test of the upper arm using a Haye's Test Chamber as follows. However, those who have experience with psoriasis, eczema, acne, and other skin allergies or who are pregnant, nursing mothers, or contraceptives and antihistamines are excluded from this experiment. After wiping the test site with alcohol and drying, the cosmetic compositions of Formulation Example 1 and Formulation Example 2 were added dropwise to the chamber (15 ㎍), respectively, and then fixed on the upper arm, which is the test site. The patch time was applied for 24 hours, and after removing the patch, the test sites were marked with a marking pen (marking pen), and the test sites were observed after 24 hours, respectively, to visually confirm the presence of allergy. As a result, it was confirmed that Formulation Example 1 and Formulation Example 2 are very safe cosmetic compositions without irritation to the skin.

Experimental Example 4. Stability Test of Cosmetic Composition Containing Blossom Mushroom Fermentation>

In order to determine the stability over time with respect to Formulation Example 2 of Example 2 according to the present invention, the following experiment was performed.

Formulation Example 2 The cosmetic composition of Example 2 was evaluated for thermal stability on a weekly basis while being stored at 0 ° C., 25 ° C., 40 ° C., a cycle thermostat, and the results are shown in the following table. Indicated.

※ Evaluation Criteria (○: Good, △: Unstable, ×: Separate) Storage condition

term 0 ℃ 25 ℃ 40 ℃ Circulating thermostat Formulation Example 2 Comparative Example 2 Formulation Example 2 Comparative Example 2 Formulation Example 2 Comparative Example 2 Formulation Example 2 Comparative Example 2 1 week ○ ○ ○ ○ ○ ○ ○ ○ 2 weeks ○ ○ ○ ○ ○ ○ ○ ○ 3 weeks ○ ○ ○ ○ ○ ○ ○ ○ 4 weeks ○ ○ ○ ○ ○ ○ ○ ○ 6 weeks ○ ○ ○ ○ ○ ○ ○ ○ 10 Weeks ○ ○ ○ ○ ○ ○ ○ ○ 12 Weeks ○ ○ ○ ○ ○ ○ ○ ○

As shown in the above table, the cosmetic composition of Preparation Example 2 and Comparative Preparation Example 2 according to the present invention was confirmed to have excellent thermal stability as a whole. In addition, it is confirmed that the mushroom mushroom fermentation extract does not affect the stability of the cosmetic composition formulation, the physical properties of the cosmetic composition to which the blossom mushroom fermentation extract is applied can be evaluated very stably.

Blossom mushroom fermentation extract according to the present invention is able to provide a safe and stable cosmetic composition with excellent antioxidant activity can be used in the cosmetics industry, and also applicable to the food and pharmaceutical industry, its industrial applicability is very large. can do. In addition, the applicability to the cosmetic composition can be stably applied to both the solubilized formulation and the emulsion formulation of the cosmetic composition can be said that the industrial applicability to the cosmetic industry using the cosmetic composition is very large.

Claims (12)

Blossom mushroom fermentation extract extracted by fermenting the fruiting body of the blossom mushroom. The method of claim 1, wherein
The fermentation broth is Lactobacillus, yeast (Yeast), photosynthetic bacteria (Photosynthetic bacteria), actinomycetes (Actinomycetes), characterized in that the composition of the mushroom mushroom fermentation extract. The method according to claim 2, wherein
Lactobacillus, yeast (Yeast), photosynthetic bacteria, Actinomycetes constituting the fermentation broth are Lactobacillus plantarum, Lactobacillus casei, Streptoccus lactis, Saccharomyces cerevisiae, Candida utilis, Streptorus repseudomonas palust A flower mushroom fermentation extract, characterized in that 1 to 5 species are selected from species such as albus, Streptomyces griseus, Aspergillus oryzae, Mucor hiemalis . 4. The method according to any one of claims 1 to 3,
Blossom mushroom fermentation extract, characterized in that the population of the fermentation microorganisms constituting the fermentation broth comprises 1.0 × 10 ⁴ cfu / mL or more for each species. Cosmetic composition containing a mushroom mushroom fermented extract. The method of claim 5
The cosmetic composition is a cosmetic composition, characterized in that 0.0001 ~ 20.0000% by weight of the mushroom mushroom fermentation extract of claim 1 based on the total weight of the cosmetic composition. The method of claim 5
The cosmetic composition is a soft cosmetic (skin toner), astringent cosmetic, nourishing cosmetic (lotion), nourishing cream, massage cream, essence, gel, pack, powder, external skin ointment, patch, suspension, emulsion, spray, Or cosmetic liquids. The method of claim 5
The cosmetic composition is a cosmetic composition, characterized in that the functional cosmetic composition, such as whitening, wrinkle improvement and sun protection. Putting the fruit mushroom body into the fermentation broth for 2 to 5 days at room temperature;
Filtering the fermented flowering mushroom fruiting body and completely concentrating with a rotary evaporator to obtain a sweet syrupy brown syrup;
Adding an aqueous 60-80% ethanol solution to the brown syrup obtained;
Concentrating the brown syrup to which the ethanol aqueous solution is added in a rotary evaporator;
Method for producing a flower mushroom fermentation extract consisting of sterilizing the concentrated brown syrup by autoclave The method of claim 9,
The fermentation broth (Lactobacillus), yeast (Yeast), photosynthetic bacteria (Photosynthetic bacteria), actinomycetes (Actinomycetes) method for producing a mushroom mushroom fermentation extract, characterized in that consisting of. The method of claim 10
Lactobacillus, yeast (Yeast), photosynthetic bacteria, Actinomycetes constituting the fermentation broth are Lactobacillus plantarum, Lactobacillus casei, Streptoccus lactis, Saccharomyces cerevisiae, Candida utilis, Streptorus repseudomonas palust A method for producing a fermented mushroom extract, characterized in that 1 to 5 species are selected from species such as albus, Streptomyces griseus, Aspergillus oryzae, Mucor hiemalis . 12. The method according to any one of claims 9 to 11,
The population of fermentation microorganisms constituting the fermentation broth is a method for producing a mushroom mushroom fermentation extract, characterized in that it comprises at least 1.0 × 10 ⁴ cfu / mL for each species.

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