KR20120086436A - Cosmetic composition for slimming - Google Patents

Abstract

The present invention is a green tea ( Camellia sinensis L.) seeds, bladder rack ( Fucus vesiculosus L.), Plantago asiatica L), safflower ( Carthamus tinctorius L.) and quinoa ( Chenopodium quinoa Willd.) Relates to a cosmetic composition, the cosmetic composition exhibits an excellent slimming effect by inhibiting triglyceride production in adipocytes.

Description

Slimming cosmetic composition {Cosmetic composition for slimming}

The present invention is a green tea ( Camellia sinensis L.) seeds, bladder rack ( Fucus vesiculosus L.), Plantago asiatica L), safflower ( Carthamus tinctorius L.) and Quinoa ( Chenopodium quinoa Willd.) Relates to a cosmetic composition, the cosmetic composition exhibits an excellent slimming effect by inhibiting triglyceride production in adipocytes.

As life improves, interest in skin care and health is increasing. Obesity is causing a lot of problems. Obesity is usually an abnormal enlargement of subcutaneous fat tissue under the skin layer. Local obesity, especially on the face, arms, thighs, buttocks and abdomen, is a condition in which fat accumulates in a specific area, which is different from obesity caused by systemic metabolic imbalances. It is caused by the greater distribution of receptors (α2-adrenaline receptors) in fat cells in the hips and thighs.

Adipose tissue is classified into white adipose tissue (WAT) and brown adipose tissue (BAT) according to its anatomical location, morphological structure, function and regulation. The process of differentiation of adipocytes is continuously converted from adipocytes to adipocytes throughout life. Differentiation from adipocytes to adipocytes shows distinct morphological and biochemical changes. Pre-established adipocytes or stromal progenitor cells derived from adipose tissue, such as 3T3-L1 cells, have been widely used to observe the process of adipocyte formation in vitro. Differentiation of adipocytes is induced by the combination of adipocyte derived complexes. By the treatment of the adipocyte-complexing cells, the cells begin to change shape, accompany some cell division, and acquire the phenotype of the adipocytes in 5-7 days.

Green tea ( Camellia sinensis In case of L.), the leaflets grown in China or Japan are 2 ~ 3m high in natural condition, and the leaflets of Assam region of India are 15m high, so they should be pruned to 0.5 ~ 1m in height. . Fruits are capsules, round and gathered, and start to grow in the spring of next year and ripen in autumn, so you can see flowers and fruits at the same time. When the fruit ripens, it bursts, resulting in a hard brown seed. Main ingredients include apigenin, astragalin, benzoaldehyde, beta-carotene, beta-nonone, beta-sitosterol, and various ingredients such as calcium, iron, magnesium, manganese, phosphorus, potassium, zinc, and vitamin B1. B2. B3. It contains B5 and C, and is known to have antioxidant, antibacterial and anticancer effects (Food Sci. Biotechnol. Vol. 15, No. 5, pp. 672-676 (2006)).

Blacus rack ( Fucus vesiculosus L.) is a brown algae distributed on the beaches of England and Ireland. Lives about 3 years and grows up to 2m. It has a flat leaf, which makes it easy to distinguish and grows in the middle rather than in deep water. High in iodine and mucus Main ingredients include aluminum, barium, bismuth, boron, calcium, chromium, cobalt, copper, gallium, iodine, iron, lithium, magnesium, manganese, nickel, phosphorus, potassium, silicon, silver, sodium, strontium, sulfur, tin, Titanium, vanadium, vitamin A, vitamin B-complex, vitamins C, E, G, S, K, zinc, zirconium and the like (Rita Berger, Hydrobiologia 514: 243-248, 2004.).

Plantago asiatica L is a perennial plant of the plantain family, and grows on the same vacant land as weeds and is distributed in Japan, Sakhalin, Okinawa, Taiwan, China, East Siberia and Malaysia. Stems are absent and leaves are spread out obliquely. A capsule is about twice as long as a calyx, and when ripened, it splits sideways, opens like a lid, and contains 6 to 8 black seeds. The medicinal herb is oval or irregularly long, slightly flat and about 2 mm long. The surface is yellowish brown or blackish brown with wrinkles, and on one side there is a little bit of grayish white seed material. The taste is sweet and the nature is cold. Contains large amounts of mucus (about 9%) and other plantenolic acid, succinic acid, adenine, choline, and in the outpost, aucubin, catalpol and methylcatalol (methylcatalpol) was isolated.

Safflower ( Carthamus tinctorius L.) is a year-old herb belonging to the Asteraceae family, native to Southeast Asia, but cultivated in India, the Far East, and Northern Africa. Later, Germany, the Netherlands, France, the Soviet Union, the United States, and Canada, followed by the United States and Mexico. Seeds are white and glossy with short tubules. The head is similar to the reddish yellow and thistle, and the muzzle has the shape of leaves, needles, and thorns like thorns. Its main ingredients are fats and oils (39-40%), protein (15%), linoleic acid (78%), oleic acid (13%), stearic acid (3%), palmitic acid (6%), and red pigment cartamine ( Carthamin, Safflor yellow, Safflomin, Luteolin-7-glucoside (leaf), Linoleic acid (50-70%, seed), Tra Celloside (Tracheloside), Mathysirinol-4-O-β-glucopyranoside (Mtciresinol-4-O-β-glucopyranoside) is mentioned.

Quinopodium quinoa Willd. Is slightly smaller than rice, and has been cultivated in the Andes, Ecuador, Peru and Bolivia, for thousands of years. Its main ingredients are rich in minerals such as protein, starch, vitamins, manganese, magnesium, iron, copper, and phosphorus, as well as potassium, vitamin-B1, vitamin-B3, vitamin-B6, vitamin-E and folic acid. It was the grain that fed the soldiers when the Inca Empire was powerful. It is a kind of herb produced in the Andes of South America. Native Americans of South America have crushed and applied it to wounds for healing.

Recently, various components for decomposing fat components accumulated in skin and body have been developed. In particular, women have more subcutaneous fat than men, and especially the arms and legs have a significant distribution, so many women want to reduce fat in certain body parts. However, in order to reckless weight loss, in addition to exercise and diet, commercial obesity programs and medications can cause economic loss or serious complications. Therefore, it is necessary to develop a formulation that can be safely applied to the human body while showing excellent slimming effect by effectively removing triglycerides.

The present invention is a green tea ( Camellia sinensis L.) seeds, bladder rack ( Fucus vesiculosus L.), Plantago asiatica L), safflower ( Carthamus tinctorius L.) and quinoa ( Chenopodium quinoa Willd.) Relates to a cosmetic composition, the cosmetic composition exhibits an excellent slimming effect by inhibiting triglyceride production in adipocytes.

The extract extracted from green tea seed, bladder rack, chajeoncho, safflower and quinoa of the present invention significantly inhibits triglyceride production in adipocytes and shows excellent slimming effect, and also shows excellent elasticity enhancing effect in skin application test.

The present invention is a green tea ( Camellia sinensis L.) seeds, bladder rack ( Fucus vesiculosus L.), Plantago asiatica L), safflower ( Carthamus tinctorius L.) and quinoa ( Chenopodium quinoa Willd.) Provides a cosmetic composition.

Each herb of the present invention includes all herb derived from the same plant.

In a specific embodiment, the composition ratio of each extract in the cosmetic composition of the present invention, namely green tea seed, bladder rack, chajeoncho, safflower, quinoa extract, is specifically 1: 1-10: 1-10: 1-10: 1-10 More specifically, about 1: 1: 5: 1 ~ 5: 1 ~ 5: 1 ~ 5, and more specifically 1: 1: 2: 1 ~ 2: 1 ~ 2: 1 ~ 2, but are not limited thereto. .

In certain embodiments, the extracts comprised in the cosmetic composition of the present invention are C ₁ to C ₄ lower alcohols such as water, ethanol, methanol, propanol, butanol, acetone, ethyl acetate, hexane, ethylene glycol, ethylene glycol, butyl It is characterized in that it is extracted using len glycol, hydrous butylene glycol, propylene glycol, hydrous propylene glycol, glycerin, hydrous glycerin or two or more mixed solvents selected from them.

In certain embodiments, the green tea seed, bladder lag, chajeoncho, safflower, and quinoa extracts are dried after all of the green tea seed, bladder rack, chajeoncho, safflower, quinoa, and about 1-20 times the raw material volume, specifically, About 10 to 15 times of water, lower alcohol or a mixed solvent having a mixing ratio of about 1: 0.1 to 1:10, specifically 1: 1 to 1: 3, 1 to 60 ° C, specifically 1 to 40 It is deposited at about 1 to 30 days at about ℃ temperature, specifically about 1 to 15 days, or about 30 minutes to 2 days at an extraction temperature of 10 ~ 100 ℃, specifically 40 ~ 100 ℃, specifically about 1 hour It can be obtained by the extraction method such as ultrasonic extraction, reflux extraction in ~ 1 day.

The extract comprises 0.001 to 10% by weight, preferably 0.1 to 8% by weight, most preferably 0.5 to 5% by weight, based on the total amount of skin oil, based on the total skin composition. More preferred.

Green tea seed, bladder rack, chajeoncho, safflower, quinoa extract obtained by the extraction method confirmed the effect of inhibiting the triglyceride production in fat cells (triglyceride) confirmed that it can be used as a slimming cosmetic composition.

In addition, any of the above-mentioned components may be blended within the range not to impair the objects and effects of the present invention, but it is preferably 0.01 to 5% by weight based on the total weight, More preferably from 0.01 to 3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture or the like.

Ingredients included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the extract as an active ingredient, for example, conventional auxiliary agents such as stabilizers, solubilizers, vitamins, pigments and flavorings. And carriers.

Hereinafter, the present invention will be described by the following Examples, Experimental Examples and Preparation Examples. However, these are provided only to more easily understand the present invention, but the scope of the present invention is not limited thereto.

Manufacturing example  1. According to the compounding ratio Green tea seed , Bladder Rack , Chachocho , Safflower, Quinoa  Preparation of Mixed Extract

Green tea seeds, bladder racks, chajeoncho, safflower, quinoa are mixed in the ratio of the following Table 1, 70% hydrous ethanol (water by weight: ethanol = 3: 7) in 10 times the amount of the raw material and the extractor at room temperature for 5 days After immersion extraction, the filtrate was filtered through a 400 mesh filter cloth, filtered through a filter of 0.45 um, and concentrated in a vacuum condenser with a cooler to prepare Comparative Examples 1 to 5 and Examples 1 to 20.

Raw material (0.1kg) Solvent amount used
(kg) Yield
(g) Green tea seed Bladder Rack Chachocho Safflower Quinoa Comparative Example 1 One - - - - One 0.2 Comparative Example 2 - One - - - One 0.4 Comparative Example 3 - - One - - One 1.1 Comparative Example 4 - - - One - One 1.4 Comparative Example 5 - - - - One One 11 Example 1 One One One One One One 2 Example 2 2 One One One One One 4 Example 3 One 2 One One One One 3 Example 4 One One 2 One One One 5 Example 5 One One One 2 One One 5 Example 6 One One One One 2 One 7 Example 7 2 2 One One One One 9 Example 8 2 One 2 One One One 13 Example 9 2 One One 2 One One 12 Example 10 2 One One One 2 One 9 Example 11 One 2 2 One One One 7 Example 12 One 2 One 2 One One 9 Example 13 One 2 One One 2 One 11 Example 14 2 2 2 One One One 10 Example 15 2 2 One 2 One One 13 Example 16 2 2 One One 2 One 17 Example 17 One 2 2 2 One One 13 Example 18 One 2 One 2 2 One 13 Example 19 One One 2 2 2 One 9 Example 20 One 2 2 2 2 One 10

Manufacturing example  2. Extraction solvent Green tea seed , Bladder Rack , Chachocho , Safflower, Quinoa  Preparation of Mixed Extract

According to the blending ratio of Example 11, 20g green tea seed, bladder rack 40g, chajeoncho 40g, safflower 20g, quinoa 20g was mixed, put into 2.0kg of each solvent of Table 2 and extracted for 5 days at room temperature with an extractor 400 mesh filter cloth Example 21 to 25 were prepared by filtration with a filter of 0.45um, and used as a sample of the following experiment.

menstruum Yield (kg) Solid content (%) Example 21 Purified water 1.8 1.7 Example 22 95% ethanol 1.8 0.2 Example 23 50% hydrous propylene glycol 1.8 0.3 Example 24 50% hydrous ethanol 1.9 0.7 Example 25 50% Water Butylene Glycol 1.7 0.6

Experimental Example  1. Measurement of fat cell toxicity

Adipocytes, 3T3-L1 cells, were cultured in DMEM containing 10% bovine calf serum. When the cell density was about 85-90%, the degree of cell activity was measured after 24 hours of Comparative Examples 1 to 5 and Examples 1 to 25 at a concentration of 100 ug / ml. As shown in Table 3, when the Comparative Examples 1 to 5 and Examples 1 to 25 were treated with the adipocyte line, no cytotoxicity was observed.

Raw material Cell survival rate (%) Raw material Cell survival rate (%) Raw material Cell survival rate (%) Comparative Example 1 100 ± 0.8 Example 6 99 ± 0.4 Example 16 103 ± 0.5 Comparative Example 2 98 ± 0.3 Example 7 101 ± 0.5 Example 17 103 ± 0.2 Comparative Example 3 99 ± 0.5 Example 8 97 ± 0.5 Example 18 99 ± 0.2 Comparative Example 4 101 ± 0.4 Example 9 107 ± 0.6 Example 19 96 ± 0.5 Comparative Example 5 101 ± 0.4 Example 10 95 ± 0.6 Example 20 96 ± 0.5 Example 1 102 ± 0.5 Example 11 105 ± 0.8 Example 21 102 ± 0.1 Example 2 101 ± 0.6 Example 12 98 ± 0.7 Example 22 99 ± 0.7 Example 3 102 ± 0.9 Example 13 99 ± 0.7 Example 23 103 ± 1.2 Example 4 101 ± 0.3 Example 14 100 ± 0.2 Example 24 103 ± 0.9 Example 5 105 ± 1.1 Example 15 106 ± 0.2 Example 25 103 ± 0.9

Experimental Example  2. Triglycerides in adipocytes triglyceride Evaluation of inhibitory effect on production

Differentiated adipocytes were incubated for 24 hours in 12-well plates at 5 × 10 ⁴ cells / well, washed once with HBSS and exchanged with medium without FBS, followed by Comparative Examples 1-5 and Examples 1-25. Samples prepared at a concentration of 100 ug / ml were added and reacted for 24 hours. The cells were washed three times with PBS and then harvested with extraction buffer (20 mM Tris, 1 mM EDTA and 1 mM 2-mercaptoethanol). The collected cells were passed six times with G26 needles on ice, then centrifuged at 500 × g, 4 ° C. for 3 minutes, and only the supernatant was taken and used for the experiment. The triglyceride (TG) was developed using a GPO-TRINDER kit purchased from Sigma (St. Louis, MO, USA), and the absorbance was measured at 540 nm with an ELISA reader. The results are shown in Table 4 below.

extract TG Production (%) Comparative Example 1 98 ± 0.2 Comparative Example 2 78 ± 0.7 Comparative Example 3 102 ± 0.4 Comparative Example 4 86 ± 0.9 Comparative Example 5 88 ± 0.5 Example 1 76 ± 0.2 Example 2 55 ± 0.4 Example 3 67 ± 0.4 Example 4 54 ± 0.2 Example 5 56 ± 1.3 Example 6 67 ± 0.7 Example 7 50 ± 1.4 Example 8 50 ± 1.0 Example 9 47 ± 0.2 Example 10 42 ± 0.9 Example 11 27 ± 0.6 Example 12 38 ± 0.2 Example 13 46 ± 0.2 Example 14 35 ± 0.6 Example 15 48 ± 1.0 Example 16 34 ± 0.9 Example 17 51 ± 1.0 Example 18 44 ± 0.4 Example 19 53 ± 0.7 Example 20 45 ± 0.7 Example 21 29 ± 0.2 Example 22 25 ± 0.4 Example 23 28 ± 1.0 Example 24 20 ± 0.4 Example 25 18 ± 0.7 Control group 100 ± 0.8

As shown in Table 4, the extract of the green tea seed, bladder rack, chajeoncho, safflower, and quinoa mixed extract 1: 2: 2: 1: 1 was significantly reduced in the concentration of triglycerides compared to the control.

Manufacturing example  3. Preparation of Skin Lotion

3-1. Manufacture (1)

2, 3, 4, and 8 were added to 11 in Table 5 in order, followed by stirring and dissolving. Then, 5 was heated to 60 ° C. for dissolution and 10 times were added thereto, followed by stirring. Finally, 1, 6, 7 and 9 were added and stirred sufficiently, and then passed through a microfluidizer and aged. Green tea seed, bladder rack, chajeoncho, safflower, quinoa mixed extract of the raw material 1 used the extract of Example 25 as a sample.

3-2. Comparative Manufacturing (1)

A skin lotion was prepared except for the first raw material of Table 5 in the same manufacturing method as in Example 25.

number Raw material Manufacturing (1) Comparative Manufacturing (1) One Extract of Example 25 5.0 - 2 glycerin 3.0 3.0 3 Butylene Glycol 2.0 2.0 4 Propylene glycol 2.0 2.0 5 Polyoxyethylene (60) Curing  Castor oil 1.00 1.0 6 ethanol 10.0 10.0 7 Triethanolamine 0.1 0.1 8 antiseptic a very small amount a very small amount 9 Pigment a very small amount a very small amount 10 Spices a very small amount a very small amount 11 Purified water Balance Balance

Manufacturing example  4. Preparation of Nutritional Lotion

4-1.Manufacturing (2)

The raw materials 2, 3, 4, 5 and 6 of Table 6 were homogenized at a constant temperature (hereinafter referred to as "nonionic amphiphilic lipids") and the raw materials 1, 7, 8 and 14 were mixed, and then Homogenized at and passed through the microfluidizer, and then the raw material 9 was slowly added and homogenized at a constant temperature, and then passed through the microfluidizer again. Thereafter, 10, 11, 12 and 13 were added to disperse to stabilize and mature. The extract of Example 25 was used as a sample.

4-2. Comparative Manufacturing (2)

Nutritional lotion was prepared by the same preparation method as Preparation Example 3-1, except for ingredient No. 1 in Table 6.

number Raw material Manufacturing (2) Comparative Manufacturing (2) One Extract of Example 25 5.0 - 2 Sitosterol 1.70 1.70 3 Polyglyceryl 2-oleate 1.50 1.50 4 Ceramide 0.7 0.7 5 Setares-4 1.2 1.2 6 cholesterol 1.5 1.5 7 Dicetyl phosphate 0.4 0.4 8 Concentrated glycerin 5.0 5.0 9 Sunflower oil 10.0 10.0 10 Carboxyvinyl polymer 0.2 0.2 11 Xanthan gum 0.3 0.3 12 antiseptic a very small amount a very small amount 13 Spices a very small amount a very small amount 14 Purified water Balance Balance

Manufacturing example  4. Preparation of Nutritional Cream

4-1. Manufacture (3)

The raw materials 2, 3, 4, 5 and 6 of Table 7 were homogenized at a constant temperature (hereinafter referred to as "nonionic amphiphilic lipids"), and the raw materials 1, 7, 8 and 14 were mixed and fixed at a constant temperature. Homogenized at and passed through a microfluidizer, and the raw material 9 was gradually added at a constant temperature to homogenize, and then passed through the microfluidizer again. Thereafter, 10, 11, 12 and 13 were added to disperse to stabilize and mature.

4-2. Comparative Manufacturing (3)

Nutritional creams were prepared except for the first raw material of Table 7 according to the same method as in Preparation Example 4-1.

number Raw material Manufacture (3) Comparative Manufacturing (3) One Extract of Example 25 5.0 - 2 Cytostolol 4.0 4.0 3 Polyglyceryl 2-oleate 3.0 3.0 4 Ceramide 0.7 0.7 5 Setares-4 2.0 2.0 6 cholesterol 3.0 3.0 7 Dicetyl phosphate 0.4 0.4 8 Concentrated glycerin 5.0 5.0 9 Sunflower oil 22.0 22.0 10 Carboxyvinyl polymer 0.5 0.5 11 Triethanolamine 0.5 0.5 12 antiseptic a very small amount a very small amount 13 Spices a very small amount a very small amount 14 Purified water Balance Balance

Experimental Example  4. Skin elasticity effect measurement test

The skin elasticity effect was measured by applying the cream of Preparation (1) and Comparative Preparation (1) of Preparation Example 3 to the skin twice daily for 30 days. The skin elasticity before sample application was measured using the cutometer SAM-474, and 25 mL / cm <2> of each sample was apply | coated to the upper arm part. Skin elasticity after 10 days, 20 days, and 30 days after application was measured. It was carried out in a constant temperature, constant humidity room controlled to a temperature of 20 ℃, 50% humidity. The test subjects were 20 males and 20s.

Duration (days) Before application 10 days later 20 days later 30 days later Manufacturing (1) application 0.922㎲ 1.009㎲ 1.122㎲ 1.101㎲ Comparative Manufacturing (1) Coating 0.920㎲ 0.922㎲ 0.923㎲ 0.924㎲

As can be seen from the results of Table 8, it was confirmed that the skin elasticity of the preparation (1) including green tea seed, bladder rack, chajeoncho, safflower, and quinoa extract was superior to that of the comparative preparation (1). None of the subjects showed unusual adverse reactions.

Claims (17)

Green tea (Camellia sinensis L.) seeds, bladder deoraek (Fucus vesiculosus L.), Plantago asiatica L), safflower ( Carthamus tinctorius L.) and Quinoa ( Chenopodium quinoa Willd.) A cosmetic composition comprising an extract extracted from the active ingredient.
According to claim 1, wherein the composition ratio of the extract extracted from green tea seed, bladder rack, chajeoncho, safflower and quinoa is 1: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10 Cosmetic composition.
According to claim 1, wherein the composition ratio of the extract extracted from green tea seed, bladder rack, chajeoncho, safflower and quinoa is 1: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5. Cosmetic composition.
According to claim 1, wherein the composition ratio of the extract extracted from green tea seeds, bladder racks, chajeoncho, safflower and quinoa is characterized in that 1: 1 to 2: 1 to 2: 1 to 2: 1 to 2: 1 to 2. Cosmetic composition.
The cosmetic composition according to claim 1, wherein the composition ratio of the extract extracted from green tea seed, bladder rack, chajeoncho, safflower and quinoa is 1: 2: 2: 1: 1.
The method of claim 1, wherein the extract is water, C _{1 -4} lower alcohols, acetone, ethyl acetate, hexane, ethylene glycol, function glycol, butylene glycol, function-butylene glycol, propylene glycol, and containing propylene glycol, glycerin, and Cosmetic composition, characterized in that extracted using one solvent or two or more mixed solvents selected from the group consisting of hydrous glycerin.
The method of claim 1, wherein the extract is a water and a C _{1 -4} lower alcohol 1: A cosmetic composition characterized in that the extraction with a solvent mixture mixed at a ratio of 0.1 to 10.
The method of claim 1, wherein the extract is a water and a C _{1 -4} lower alcohol 1: A cosmetic composition characterized in that the extraction with a solvent mixture mixed at a ratio of 1 to 3.
The cosmetic composition according to claim 1, comprising 0.001 to 10% by weight of the extract based on the total weight of the cosmetic composition.
The cosmetic composition according to claim 1, comprising 0.1 to 8% by weight of the extract based on the total weight of the cosmetic composition.
The cosmetic composition according to claim 1, comprising 0.5 to 5% by weight of the extract based on the total weight of the cosmetic composition.
Slimming cosmetic comprising the cosmetic composition of any one of claims 1 to 11 as an active ingredient.
A cosmetic for enhancing skin elasticity, comprising the cosmetic composition of any one of claims 1 to 11 as an active ingredient.
Add green tea seed, bladder rack, chajeoncho, safflower and quinoa dry samples to 1-20 times the total weight of the extraction solvent,
To be deposited under temperature conditions of 1 to 60 ° C; or
Method for producing a cosmetic composition, characterized in that the extraction under the temperature conditions of 10 to 100 ℃.
15. The method of claim 14 wherein the extraction solvent from water, C _{1 -4} lower alcohols, acetone, ethyl acetate, hexane, butylene glycol, function-butylene glycol, propylene glycol, and containing propylene glycol, glycerin and the function groups of glycerol Method of producing a cosmetic composition, characterized in that the selected solvent or two or more mixed solvents.
15. The method of claim 14 wherein the extraction solvent is water and the lower alcohol C _{1 -4} 1: Preparation of a cosmetic composition, characterized in that a mixed solvent were mixed in a ratio of from 0.1 to 10.
15. The method of claim 14 wherein the extraction solvent is water, a lower alcohol and a C _{1 -4} 1: Preparation of a cosmetic composition, characterized in that a mixed solvent were mixed in a ratio of 1 to 3.