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KR20120031862A - A liquified mixing method of sulfur and em(effective micro organism) for preventing epidemic and exterminating of insect pests, and thereof a composite - Google Patents

A liquified mixing method of sulfur and em(effective micro organism) for preventing epidemic and exterminating of insect pests, and thereof a composite Download PDF

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KR20120031862A
KR20120031862A KR1020110042295A KR20110042295A KR20120031862A KR 20120031862 A KR20120031862 A KR 20120031862A KR 1020110042295 A KR1020110042295 A KR 1020110042295A KR 20110042295 A KR20110042295 A KR 20110042295A KR 20120031862 A KR20120031862 A KR 20120031862A
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sulfur
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microorganisms
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박경희
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(주)황샘바이오
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Priority to KR1020110117809A priority patent/KR101143903B1/en
Priority to PCT/KR2011/009936 priority patent/WO2012150752A1/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/02Sulfur; Selenium; Tellurium; Compounds thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20Bacteria; Substances produced thereby or obtained therefrom
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Wood Science & Technology (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental Sciences (AREA)
  • Toxicology (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

본 발명의 방제 방역 및 작물 생육용으로 이용하기 위하여 제독되지 않은 유황과 유용미생물군을 액상혼합하는 방법 및 그 조성물은 유용미생물군(EM : Effective Micro organism)과 제독되지 않은 유황분말을 1 : 1 내지 20 : 1의 부피비율로 혼합한 액상혼합액을 아크릴관이나 스텐레스 보호통 안에 60℃정도의 기름이나 물을 채우고 전기가열기를 넣어 간접 가열하여 35℃ ~ 60℃ 온도가 유지된 상태에서 24시간 방치하는 단계; 이 후 7일 ~ 14일간 매일 1 ~ 5회씩 회당 1 ? 20분간 제독되지 않은 유황과 유용미생물군(EM)의 액상혼합액을 나무막대기로 충분히 교반하는 단계와; 상기 액상혼합액 99.8중량%에 구연산 0.2중량% 혼합하는 단계로 이루어져 있어, 물에 용해되지 않는 제독되지 않은 유황을 유용미생물군(EM : Effective Micro organism)과 액상혼합하여 작물의 방제를 위한 효과는 물론 작물의 세포막을 튼튼하게 해주고 영양분의 세포 내 흡수를 원활하게 도와주어 작물을 잘 자라나게 할 뿐만 아니라 강 항산화 작용을 통해 작물의 보존성을 높여주며 증수 효과 및 품질이 향상되고, 산성을 띠는 구연산을 통해 유용미생물군(EM)에 포함되어 있는 유해한 미생물을 사멸시키면서 유용 미생물을 활성화시켜 유용미생물의 역가를 높일 수 있는 방제 방역 및 작물 생육용으로 이용하기 위하여 제독되지 않은 유황과 유용미생물군을 액상혼합하는 방법 및 그 조성물을 제공한다.Method for the liquid-mixing of the toxic decontaminated sulfur and the useful microorganism group for use in the control and crop growth of the present invention and the composition of the effective microorganism (EM) and toxic decontaminated sulfur powder 1: 1 To 20: 1 mixed liquid mixture in a volume ratio of 60 ℃ in the acrylic tube or stainless steel protective container filled with oil or water of about 60 ℃ and indirect heating by placing an electric heater and left for 24 hours in a state maintained at 35 ℃ ~ 60 ℃ temperature Doing; After that, 1 to 5 times per day for 7 to 14 days. Sufficiently stirring the liquid mixture of sulfur and non-toxic microorganism group (EM) for 20 minutes with a wooden stick; It consists of mixing 0.29.8% citric acid in 99.8% by weight of the liquid mixture, the liquid is not dissolved in water in the liquid mixed with an effective microorganism (EM: Effective Micro organism) of course to control the crop of course It strengthens the cell membranes of crops and facilitates the absorption of nutrients into the cells to help grow crops well. It also increases the preservation of crops through strong antioxidant activity, improves the effectiveness and quality of water, and produces citric acid. Liquid-mixing of uncontaminated sulfur and useful microorganisms for use in prevention and prevention and crop growth that can increase the titer of useful microorganisms by killing harmful microorganisms contained in the useful microorganisms (EM) It provides a method and a composition thereof.

Description

방제 방역 및 작물 생육용으로 이용하기 위하여 제독되지 않은 유황과 유용미생물군을 액상혼합하는 방법 및 그 조성물{A liquified mixing method of sulfur and EM(Effective Micro Organism) for preventing epidemic and exterminating of insect pests, and thereof a composite}A liquified mixing method of sulfur and EM (Effective Micro Organism) for preventing epidemic and exterminating of insect pests, and know a composite}

본 발명은 작물에 발생하는 방제 및 방역을 위해 제독되지 않은 유황과 유용미생물군을 액상혼합하는 방법 및 그 조성물에 관한 것으로, 더욱 상세하게는 물에 용해되지 않는 제독되지 않은 유황을 유용미생물군(EM : Effective Micro organism)과 액상혼합하여 작물의 방제를 위한 효과는 물론 작물의 세포막을 튼튼하게 해주고 영양분의 세포 내 흡수를 원활하게 도와주어 작물을 잘 자라나게 할 뿐만 아니라 강 항산화 작용을 통해 작물의 보존성을 높여주며 증수 효과 및 품질이 향상되고, 산성을 띠는 구연산을 통해 유용미생물군(EM)에 포함되어 있는 유해한 미생물을 사멸시키면서 유용 미생물을 활성화시켜 유용미생물의 역가를 높일 수 있는 방제 방역 및 작물 생육용으로 이용하기 위하여 제독되지 않은 유황과 유용미생물군을 액상혼합하는 방법 및 그 조성물에 관한 것이다.
The present invention relates to a method and a composition for liquid-mixing undetermined sulfur and a useful microorganism group for the control and control of crops. More particularly, the present invention relates to a non-detoxified sulfur which does not dissolve in water. Liquid mixing with EM (Effective Micro organism) not only helps to control crops, but also strengthens the cell membranes of the crops and facilitates the absorption of nutrients into the cells. Control and prevention to increase the titer of useful microorganisms by activating useful microorganisms by killing harmful microorganisms contained in the useful microorganisms (EM) through increased preservation, improved water efficiency and quality, and acidic citric acid. Method for liquid-mixing undetoxified sulfur with useful microorganisms for use in growing crops It relates to the sacred.

유황은 토양의 7대 필수 영양소 중 하나로서 모든 식물의 질소공급원인 동시에 특유의 독성을 지니고 있어 해충과 유해균 방제에도 효과가 탁월하다고 알려져 있으며, 최근 삶의 질이 높아지면서 소비자들이 유기농산물을 많이 찾게 되자 경작자들은 화학비료나 농약을 사용하지 않는 친환경 농산물 생산을 위하여 농약 대신 유황을 해충과 유해균 방제에 유황을 이용하는 농가가 확산되고 있다. Sulfur is one of the seven essential nutrients in the soil and is known to be excellent in controlling pests and harmful bacteria because it is a nitrogen source of all plants and also has unique toxicity.In recent years, the quality of life has increased and consumers are looking for organic products. As a result, farmers are using sulfur instead of pesticides to control pests and harmful bacteria to produce eco-friendly agricultural products that do not use chemical fertilizers or pesticides.

이렇게 농약대신 유황으로 효과적인 약제방제를 하려면 유황을 모든 식물에 효과적으로 도포하기 위하여 액상화가 선행되어야 하나 유황은 물에 용해되지 않기 때문에 분말형태로 식물과 토양에 직접 뿌려줄 수밖에 없는데 이럴 경우 유황분말이 작물에 골고루 점착되지 않아 방제효과가 떨어질 뿐 아니라 분말형태이므로 식물의 흡수율도 떨어져 필요한 시기에 질소공급원으로서의 원활한 기능도 기대할 수가 없는 것이다.In order to effectively control sulfur with sulfur instead of pesticides, liquefaction must be preceded in order to effectively apply sulfur to all plants, but since sulfur is not soluble in water, it can only be sprayed directly on plants and soil in the form of powder. As it is not evenly adhered, the control effect is not only lowered, but also because it is in powder form, the absorption rate of the plant is also lowered, and thus a smooth function as a nitrogen supply source cannot be expected.

아울러, 분말유황은 또한 작업자가 부주의로 특정지역, 특정부위에 적정량을 초과하여 과량 살포할 경우 그 주변의 작물과 토양미생물에 치명적일 수 있으며, 분말 형태의 유황은 광역살포가 가능한 액상에 비하여 작업 능률이 떨어지므로 결국은 생산비용 상승의 요인이 되는 것이다.In addition, powdered sulfur can also be fatal to crops and soil microorganisms around them when the worker inadvertently spreads excess amount to a specific area or region, and powdered sulfur is more efficient than a liquid that can be sprayed in a wide area. This eventually leads to a rise in production costs.

이렇게 유황은 모든 식물의 질소공급원으로서 영양소적 기능과 해충, 유해균을 퇴치, 사멸하는 약제방제의 기능도 가지고 있으나 분말형태의 유황은 액상에 비하여 식물의 원활한 흡수를 기대하기가 어렵고 식물의 잎이나 줄기에 적정 수준까지 유착되지 않아 지속인 영양공급과 약제방제를 수행하기 어려운 문제점이 있었다.As such, sulfur is a nitrogen source of all plants, and it has the function of nutrients and the control of pests and harmful bacteria. However, sulfur in powder form is hard to expect smooth absorption of plants compared to liquids and leaves or stems of plants. There was a problem that it is difficult to carry out continuous nutrition and drug control because they did not coalesce to an appropriate level.

이에 따라, 종래에는 물에 용해되지 않는 유황을 액상화 할 목적으로 석회와 유황을 혼합, 고열로 끓여서 점액질 상태(석회유황합제)로 만들어 20 ? 40cc를 20리터의 물에 풀어서 사용하여 왔다. Thus, lime and sulfur are conventionally mixed and boiled with high heat to make liquefied sulfur (lime sulfur mixture) for the purpose of liquefying sulfur insoluble in water. 40 cc of water has been used in 20 liters of water.

또한, 최근에는 유황분말을 액상화 하기 위하여 세탁비누 액 또는 주방세제 등을 유황분말과 함께 믹서기로 고속 회전하여 물에 풀어지게 하는 콜로이드 화 방법을 쓰기도 하였다. Recently, in order to liquefy sulfur powder, a colloidal method for washing laundry soap solution or dish detergent with sulfur powder at high speed with a blender to be released in water has been used.

아울러, 당밀을 계면활성제 역할을 하게 할 목적으로 당밀과 유황분말을 믹서기로 고속 회전하여 점액질화 한 후 물에 풀어서 사용하고 있는데 이 방법은 당밀의 당분에 의해 오히려 유해균과 해충이 군집하는 문제점이 나타나 일부 경작자들은 유해균, 해충의 군집을 예방하기 위하여 당밀에 과산화수소, 염소 따위를 첨가하고 있는 실정이다.
In addition, molasses and sulfur powder is mucogenized by rotating the molasses and sulfur powder at high speed with a mixer for the purpose of acting as a surfactant, and this method is used to release harmful bacteria and pests by molasses sugar. Some farmers are adding hydrogen peroxide and chlorine to molasses to prevent harmful bacteria and pest populations.

하지만, 종래 유황을 가열하여 액상화하는 방법은 유황을 가열하는 과정에서 악취 및 유독가스가 발생, 작업자의 건강과 안전을 위협하는 등 과정이 매우 번거로웠다.However, the conventional method of heating and liquefying sulfur has been very cumbersome, such as odor and toxic gas, which threatens the health and safety of workers.

아울러, 유황분말을 액상화 하기 위하여 콜로이드화 하는 방법은 세탁비누 액이나 주방세제가 토양과 하천에 또 다른 오염원이 될 문제점이 있었으며, 소비자 또한 세탁비누나 주방세제가 묻은 농산물을 기피하게 되어 문제점으로 노출되었다.In addition, the colloidal method for liquefying sulfur powder has a problem that the laundry soap solution or dish detergent become another pollutant in the soil and rivers, and consumers have also been exposed to problems by avoiding the laundry soap or dish detergent. .

또한, 당밀의 당분에 의해 유해균과 해충이 군집하는 문제점을 해결하려고 당밀에 과산화수소, 염소 따위를 첨가해야 하는데 이는 식물의 세포를 손상케 하는 치명적인 물질이기 때문에 이 방법도 바람직하지 않다는 결론에 도달하였다.
In addition, hydrogen peroxide, chlorine, etc. should be added to molasses in order to solve the problem of harmful bacteria and pests gathered by molasses sugar, and this method is not preferable because it is a fatal substance that damages cells of plants.

상기와 같은 문제점을 해결하기 위한 본 발명의 방제 방역 및 작물 생육용으로 이용하기 위하여 제독되지 않은 유황과 유용미생물군을 액상혼합하는 방법 및 그 조성물은 유용미생물군(EM : Effective Micro organism)과 제독되지 않은 유황분말을 1 : 1 내지 20 : 1의 부피비율로 혼합한 액상혼합액을 아크릴관이나 스텐레스 보호통 안에 60℃정도의 기름이나 물을 채우고 전기가열기를 넣어 간접 가열하여 35℃ ~ 60℃ 온도가 유지된 상태에서 24시간 방치하는 단계와; 이 후 7일 ~ 14일간 매일 1 ~ 5회씩 회당 1 ? 20분간 제독되지 않은 유황과 유용미생물군(EM)의 액상혼합액을 나무막대기로 충분히 교반하는 단계와; 상기 액상혼합액 99.8중량%에 구연산 0.2중량% 혼합하는 단계로 이루어진 것을 특징으로 한다.
The method and composition of the liquid-mixed sulfur and the useful microorganisms that are not detoxified for use in the control and crop growth of the present invention for solving the above problems are effective microorganisms (EM) and detoxification Fill the liquid mixture of uncontained sulfur powder in a volume ratio of 1: 1 to 20: 1 by filling oil or water at about 60 ℃ in an acrylic tube or stainless steel protective container, and indirectly heating it by putting an electric heater to heat the temperature between 35 ℃ and 60 ℃. Leaving for 24 hours in a state where it is maintained; After that, 1 to 5 times per day for 7 to 14 days. Sufficiently stirring the liquid mixture of sulfur and non-toxic microorganism group (EM) for 20 minutes with a wooden stick; 99.8% by weight of the liquid mixture is characterized in that the step consisting of mixing 0.2% by weight citric acid.

본 발명은 물에 용해되지 않는 제독되지 않은 유황을 유용미생물군(EM)과 액상혼합하여 작물의 방제를 위한 효과는 물론 작물의 세포막을 튼튼하게 해주고 영양분의 세포 내 흡수를 원활하게 도와주어 작물을 잘 자라나게 할 뿐만 아니라 강 항산화 작용을 통해 작물의 보존성을 높여주며 증수 효과 및 품질 향상이 되는 효과가 있다.The present invention liquid-mixed undetermined sulfur, which is not soluble in water, with a useful microorganism group (EM) to effectively control crops, as well as to strengthen the cell membranes of crops and to facilitate the absorption of nutrients into the cells. Not only does it grow well, it also increases the preservation of crops through strong antioxidant activity, and increases the water effect and quality.

아울러, 산성을 띠는 구연산을 통해 유용미생물군(EM)에 포함되어 있는 유해한 미생물을 사멸시키면서 유용 미생물을 활성화시켜 유용미생물의 역가를 높일 수 있는 유용한 발명이다.
In addition, it is a useful invention to increase the titer of useful microorganisms by activating the useful microorganisms while killing harmful microorganisms contained in the useful microorganism group (EM) through the acidic citric acid.

먼저, 제독되지 않은 유황을 분말형태로 만든 다음 점액질을 가지는 방선균과 제독되지 않은 유황을 먹이로 활동하는 일부 유산균, 효모균, 광합성균 등을 포함하는 유용미생물 군, 즉, 유용미생물군(EM)의 균을 혼합한다.First of all, the microorganisms of the microorganisms, ie, the microorganisms (EM), which are made of powdered non-toxic sulfur and then some of the actinomycetes having mucus and some lactic acid bacteria, yeasts, photosynthetic bacteria, etc., which feed on untoxic sulfur, Mix the bacteria.

상기와 같이 유용미생물군(EM)과 제독되지 않은 유황분말을 혼합할 때 유용미생물군(EM)과 제독되지 않은 유황분말의 비율은 1 : 1 내지 20 : 1의 부피비율로 혼합한다. When the useful microorganism group (EM) and the non-detoxified sulfur powder are mixed as described above, the ratio of the useful microorganism group (EM) and the non-detoxified sulfur powder is mixed at a volume ratio of 1: 1 to 20: 1.

상기의 비율로 액상혼합된 상태에서 5℃ ~ 60℃ 온도가 유지되도록 하면서 24시간을 방치하면, 제독되지 않은 유황이 유용미생물군(EM) 활성액과 닿는 아래 부분부터 유용미생물군(EM)의 점성과 제독되지 않은 유황을 먹이로 활동하는 광합성균 등으로 인하여 서서히 가라앉기 시작한다. When left for 24 hours while maintaining a temperature of 5 ° C. to 60 ° C. in a liquid-mixed state at the above ratio, the viscosity of the useful microorganism group (EM) starts from the lower portion where the undetoxified sulfur comes into contact with the useful microorganism group (EM) active liquid. It begins to sink slowly due to photosynthetic bacteria, which act as food for the larvae and undetoxified sulfur.

이 후 2일째부터는 7일 ~ 14일간 매일 1 ~ 5회씩 회당 1 ? 20분간 제독되지 않은 유황과 유용미생물군(EM)의 액상혼합액을 나무막대기로 충분히 저어주는데 이는, 유용미생물군(EM)의 균 중 호기성 미생물의 활성화를 위하여 저어줄 때 적정량의 공기가 들어가게 하여 활성을 유도함과 동시에 유용미생물군(EM) 속의 제독되지 않은 유황을 서로 뒤집어 줌으로써 제독되지 않은 유황이 유용미생물군(EM)과 빨리 혼합되게 하기 위한 것이다. From the second day onwards, 1 to 5 times per day for 7 to 14 days. Stir the liquid mixture of uncontaminated sulfur and useful microorganisms (EM) for 20 minutes with a wooden stick. When stirring for the activation of aerobic microorganisms among the bacteria of the useful microorganisms (EM), an appropriate amount of air enters By inverting the uncontaminated sulfur in the useful microorganisms (EM) and inverting each other, the non-detoxified sulfur is quickly mixed with the useful microorganisms (EM).

유용미생물군(EM)의 균 중 식물의 광합성 활동에 유용하고 식물을 단단하고 건강하게 해주는 미생물인 광합성 균은 35℃~60℃에서 활성도가 가장 높고 온도가 내려갈수록 활성이 둔화되다가 5℃이하에서 생명활동이 거의 정지하므로 항상 35℃~60℃를 유지하는 것이 바람직하다.Among the microorganisms of the useful microorganism (EM), photosynthetic bacteria, which are useful for photosynthetic activity of plants and make plants hard and healthy, have the highest activity at 35 ℃ ~ 60 ℃ and slow down as temperature decreases. It is desirable to maintain 35 ~ 60 ℃ at all times because life activity is almost stopped.

물론 유산균, 효모 등은 60℃ 이상에서도 활성도가 높기는 하나 식물의 생장, 발육을 위하여 광합성균이 꼭 필요하므로 유용미생물군(EM) 활성액의 최고온도는 60℃를 초과하지 말아야 하는 것이다. Of course, lactic acid bacteria, yeast, etc., even if the activity is higher than 60 ℃, photosynthetic bacteria are necessary for the growth and growth of plants, so the maximum temperature of the useful microbial group (EM) active liquid should not exceed 60 ℃.

이와 같이 유용미생물군(EM) 활성액의 적정온도를 유지하기 위하여 온도조절장치가 부착된 전기 가열기를 이용하면 작업자가 현장을 떠나있어도 원하는 온도를 계속 유지할 수가 있어 편리하다.Thus, using an electric heater equipped with a temperature control device to maintain the proper temperature of the useful microbial group (EM) active liquid is convenient to maintain the desired temperature even if the worker leaves the site.

이때, 전기 가열기를 유용미생물군(EM) 활성액에 직접투입, 가열하게 되면 전기 가열기에 근접한 미생물이 고온으로 인하여 사멸할 우려가 있으므로 아크릴관이나 스텐레스 보호통 안에 60℃정도의 기름이나 물을 채우고 전기가열기를 넣어 간접 가열하는 것이 바람직하다.At this time, if the electric heater is directly injected into the useful microorganism group (EM) active liquid and heated, the microorganisms close to the electric heater may be killed due to the high temperature, so the oil or water of about 60 ° C. is filled in the acrylic tube or the stainless steel protective container. It is preferable to add hot air and indirect heating.

여기서, 상기 유용미생물군(EM) 중 유산균 종류들은 pH 4 근처의 산성을 띨때 활동성이 가장 강하게 작용하게 되는데, 이러한 유용한 유산균들은 유해한 미생물들의 생육을 억제하거나 혹은 먹이 활동을 저해하므로 유해미생물을 사멸시키는 역할을 하게 된다.Here, the lactic acid bacteria species of the useful microorganism group (EM) is the most active when the acidity near the pH 4, these useful lactic acid bacteria to kill harmful microorganisms by inhibiting the growth of harmful microorganisms or feeding activity It will play a role.

한편, 상기와 같이 혼합된 액상혼합액 99.8중량%에 구연산 0.2중량%를 혼합하여 교반한다.Meanwhile, 0.29.8% citric acid is mixed with 99.8% by weight of the liquid mixture mixed as described above, followed by stirring.

여기서, 상기 구연산은 미농무성, 호주농무성, 우리나라의 국립수의과학검역원에서 살균효과와 살바이러스(구제역 등) 효과를 인증한 제품으로 산성을 띠게 되어 유용미생물군(EM) 중에 포함되어 있는 유해미생물을 사멸시키는 작용을 하게 되고, 이와 동시에 제독되지 않은 유황은 유용미생물군(EM)에 포함되어 있는 미생물들의 활동을 도와주는 역할을 함으로 인해 유용미생물의 역가를 높여주는 작용을 하게 된다.Here, the citric acid is a product certified by the USDA, USDA, the National Veterinary Research and Quarantine Service of Korea, the bactericidal effect and the killer virus (foot-and-mouth disease, etc.) effect is acidic to contain harmful microorganisms contained in the useful microorganism group (EM) At the same time, the non-detoxified sulfur acts to help the microorganisms contained in the useful microorganism (EM), thereby increasing the potency of the useful microorganism.

상기와 같은 방법으로 제조된 본 발명의 방제 방역 및 작물 생육용으로 이용하기 위하여 제독되지 않은 유황과 유용미생물군(EM)을 액상혼합 방법에 의한 조성물은 일반 물에 잘 희석이 되어 기존의 작물에 직접 살포할 수 있으며, 본원의 제독되지 않은 유황과 유용미생물군(EM)의 액상혼합액이 살포된 작물은 해충 중 진딧물의 경우 3일 후 76.0%, 7일 후 82.9%의 방제효과가 나타났다.In order to use for the control and crop growth of the present invention prepared by the above method, the composition by the liquid mixing method of sulfur and useful microorganisms (EM) that have not been detoxified is well diluted in ordinary water to The crops that can be directly sprayed and sprayed with the liquid mixture of the uncontaminated sulfur and the useful microorganism group (EM) of the present application showed control effects of 76.0% after 3 days and 82.9% after 7 days for aphids among pests.

아울러, 유해균 중 흰가루병의 경우 작물 살포 7일 후 85.9%의 방제효과가 나타나는 것으로 관찰되었고, 별도의 유용미생물(EM)을 통해 영양공급을 할 수 있어 작물의 세포막을 튼튼하게 해주고 영양분의 세포 내 흡수를 원활하게 도와주어 작물을 잘 자라나게 할 뿐만 아니라 강 항산화 작용을 통해 생산 작물의 보존성을 높여주며 증수 효과 및 품질이 향상되는 효과가 나타났다.In addition, in the case of powdery mildew among harmful bacteria, 85.9% of control effect was observed after 7 days of crop spraying, and it is possible to supply nutrition through a separate useful microorganism (EM) to strengthen the cell membrane of the crop and absorb nutrients into the cells. In addition to helping the crops to grow well, strong antioxidants have been shown to increase the preservation of the produced crops, as well as increase the yield and quality.

이하, 본 발명에 따르는 해충과 유해균의 방제를 위해 제독되지 않은 유황과 유용미생물군을 액상혼합 방법 및 이에 따른 액상혼합액의 실시예를 설명하면 다음과 같다.Hereinafter, the sulfur and the useful microorganisms which are not detoxified for the control of pests and harmful bacteria according to the present invention will be described with examples of liquid mixing method and liquid mixture according to the embodiment.

<실시예 1>&Lt; Example 1 >

고추의 Pepper 목화진딧물Cotton Aphid 방제시험 Control test

본 실험은 공주대학교 산업과학대학에 의뢰하여 나타난 자료를 토대로 작성하였다.This experiment was prepared based on the data presented by Gongju University.

1. 시험목적 : 우수한 제제를 선발하여 작물해충 관리용 자재로 활용코자 함.1. Purpose of Test: To select excellent formulations and use them as materials for crop pest management.

2. 시험방법 2. Test method

가. 시험작물(품종) : 고추(녹광)end. Test crops (variety): red pepper (green mine)

나. 대상해충 : 고추 목화진딧물(Aphis gossypii)I. Pests: Aphis gossypii

다. 대상해충 발생상황 : 약제처리 전 무처리구 발생밀도가 구당 평균 82.5마리로 약제간의 방제효과를 검토하기에 충분하였음.All. Pest incidence: The mean incidence of untreated group before treatment was 82.5 animals per group, which was sufficient to examine the control effect between drugs.

라. 경종개요 : 일반 농가 관행재배법에 준하였음. 다른 해충의 방제를 위한 약제 살포는 없었음.la. Seedling overview: In accordance with the common farming practices. There was no pharmaceutical spray for the control of other pests.

마. 시험구 배치 및 면적 : 난괴법 3반복hemp. Test Arrangement and Area: 3 times of ingot method

구분 처리수 반복수 종구수 구당면적 소요면적 총소요면적Classification Number of treatments Number of repeats Number of balls Number of squares required area Total area required 약효 3 3 9 20㎡ 180㎡
225㎡
약해 3 3 9 5㎡ 45㎡
Efficacy 3 3 9 20㎡ 180㎡
225㎡
Weak 3 3 9 5㎡ 45㎡

바. 처리내용bar. Processing contents

주성분
시험약제 함 량
(%)

제독되지 않은유황 유황20
(황+유용미생물+ 미생물79.8
구연산) 구연산 0.2

치아클로프리드 thiacloprid10
액상수황제(대조)

무처리
chief ingredient
Test drug content
(%)

Un-Admired Sulfur Sulfur20
(Sulfur + Useful Microorganism + Microorganism 79.8
Citric acid) citric acid 0.2

Chia clofried thiacloprid10
Liquid Sulfur Detergent (Control)

No treatment
약효시험 약해시험
Drug Test Drug Test

의뢰기관

(주)황샘바이오

Client

Hwang Saem Bio Co., Ltd.
희석배수 처리시기 기준량 배량
및 사용량 및 방법

1,000배 발생기 1회 1,000배 500배
경엽처리 (6월15일)(6월15일) (6월15일)

2,000배


Dilution rate treatment time
And usage and methods

1,000 times 500 times of generators 1,000 times
Foliage Treatment (June 15) (June 15) (June 15)

2,000 times


사. 약제살포 전후 기상상황four. Weather situation before and after drug spraying

약효에 영향을 미칠만한 특별한 기상상황은 없었음.There were no special weather conditions that could affect the efficacy.

3. 조사방법3. Survey Method

구분 조사항목 조사횟수 조사일자 조사방법Classification Survey Items Survey Frequency Survey Date Survey Method 약효시험 생충율 3회 6월15일 약제처리 전 및 약제처리 3, 7일 후
18일, 22일 구당 10주에 대한 생충수 조사
약해시험 약해유무 3회 상동 외관상 약해유무 달관조사(0-5)
Drug efficacy test 3 times before June 15 and after 3 and 7 days of drug treatment
Investigation of viable numbers for 10 weeks per day on 18 and 22 days
Test for weakness 3 times of weakness Test of investigation on the appearance of weakness in Sangdong (0-5)

4. 시험성적4. Test score

가. 약효시험end. Drug test

- 고추 목화진딧물에 대한 약제방제 효과(약제처리 3일 후)-Pharmaceutical control effect on red pepper aphid (3 days after drug treatment)

처리전
시험약제 밀도
(마리/구)
Before treatment
Test Agent Density
(Mari / ward)
생충율(%)            % Viability
유의차-5% 방제가
(DMRT) (%)

Significant difference-5% control
(DMRT) (%)
1반복 2반복 3반복 평균1 Repeat 2 Repeat 3 Repeat Average 제독되지 않은유황
(황+유용미생물+ 82.7 24.8 25.8 21.4 24.0 a 76.0
구연산)
치아클로프리드
액상수화제(대조) 83.3 12.6 8.5 10.4 10.5 a 89.5
무처리 82.5 97.6 105.4 98.4 100.5 b -
Un-Admired Sulfur
(Sulfur + useful microorganisms + 82.7 24.8 25.8 21.4 24.0 a 76.0
Citric acid)
Chicloprid
Liquid Hydrating Agent (Control) 83.3 12.6 8.5 10.4 10.5 a 89.5
Untreated 82.5 97.6 105.4 98.4 100.5 b-

- 고추 목화진딧물에 대한 약제방제 효과(약제처리 7일 후)-Pharmaceutical control effect on red pepper aphid (7 days after drug treatment)

처리전
시험약제 밀도
(마리/구)
Before treatment
Test Agent Density
(Mari / ward)
생충율(%)            % Viability
유의차-5% 방제가
(DMRT) (%)

Significant difference-5% control
(DMRT) (%)
1반복 2반복 3반복 평균1 Repeat 2 Repeat 3 Repeat Average 제독되지 않은 유황
(황+유용미생물+ 82.7 18.7 16.3 16.4 17.1 a 82.9
구연산)
치아클로프리드
액상수화제(대조) 83.3 12.5 10.4 13.2 12.0 a 88.0
무처리 82.5 101.4 109.8 104.9 105.4 b -
Un-Admired Sulfur
(Sulfur + useful microorganisms + 82.7 18.7 16.3 16.4 17.1 a 82.9
Citric acid)
Chicloprid
Liquid Hydrating Agent (Control) 83.3 12.5 10.4 13.2 12.0 a 88.0
Untreated 82.5 101.4 109.8 104.9 105.4 b-

나. 약해시험I. Weakness test

-약제처리 후 3, 5, 7일 후 조사3, 5, 7 days after drug treatment

시험약제 시험작물
(품종)
Test drug
(kind)
약해정도(0-5)      Weak degree (0-5) 비 고
Remarks
기준량 배량Baseline 제독되지 않은유황 고 추
(황+유용미생물+ (녹광)
구연산)
Unadmired Sulfur Pepper
(Sulfur + Useful Microorganism + (Green)
Citric acid)
0 0    0 0 약해없음No weakness

5. 결과 요약5. Summary of Results

가. 약효end. Medicinal effect

고추 목화진딧물에 대한 시험약제인 제독되지 않은 유황(황+유용미생물+구연산)은 약제처리 3일 후 76.0%, 약제처리 7일 후 82.9%의 방제효과를 보였음.Non-detoxified sulfur (sulfur + useful microorganism + citric acid), a test drug for red pepper aphid, showed 76.0% after 3 days of treatment and 82.9% after 7 days of treatment.

나. 약해I. Weak

상기 시험약제의 기준량 및 배량을 각각 경엽 처리한 후 3회에 걸쳐 약해유무를 달관 조사한 결과 약제처리에서 증상이 관찰되지 않았음.As a result of investigating whether the base drug and the dose of the test drug were treated with leaves three times after each leaf treatment, no symptoms were observed in the drug treatment.

6. 시험담당자 의견6. Examiner Comments

시험약제가 고추 목화진딧물에 대한 방제약제로 실용성이 있다고 판단됨.
The test drug is considered to be practical as a control agent for pepper cotton aphids.

<실시예 2><Example 2>

딸기 흰가루병 방제시험Strawberry powdery disease control test

본 실험은 공주대학교 산업과학대학에 의뢰하여 나타난 자료를 토대로 작성하였다.This experiment was prepared based on the data presented by Gongju University.

1. 시험목적 : 우수한 제제를 선발하여 작물병해 관리용 자재로 활용코자 함.1. Purpose of the Test: To select a good formulation and use it as a material for crop disease management.

2. 시험방법2. Test method

가. 시험작물(품종) : 딸기(매향)end. Test crops (varieties): Strawberry

나. 대상병해 : 딸기 흰가루병(Sphaerotheca fuliginea)I. Target disease: Strawberry powdery mildew (Sphaerotheca fuliginea)

다. 대상병해 발생상황 : 약제 처리 전 무처리구 딸기 흰가루병의 발병도는 구당 46.5로 약제간의 방제효과를 검토하기에 충분하였음.All. Condition of disease: The incidence of strawberry powdery mildew was 46.5 per ward before treatment.

라. 경종 개요 : 일반농가 관행재배법에 준하였음. 다른 병해의 방제를 위한 약제 살포는 없었음.la. Seedling Overview: Compliant with the common farming practices. There was no pharmaceutical spray for the control of other diseases.

마. 시험구 배치 및 면적 : 난괴법 3반복hemp. Test Arrangement and Area: 3 times of ingot method

구분 처리수 반복수 총구수 구당면적 소요면적 총소요면적Classification Number of treatments Number of repetitions Total number of spheres Area required Area Total area required 약효 3 3 9 20㎡ 180㎡
225㎡
약해 3 3 9 5㎡ 45㎡
Efficacy 3 3 9 20㎡ 180㎡
225㎡
Weak 3 3 9 5㎡ 45㎡

바. 처리내용bar. Processing contents

주성분
시험약제 함 량
(%)

제독되지 않은유황 유황20
(황+유용미생물+ 미생물79.8
구연산) 구연산0.2


훼나리유제 fenarimol 12.5(대조)

무처리
chief ingredient
Test drug content
(%)

Un-Admired Sulfur Sulfur20
(Sulfur + Useful Microorganism + Microorganism 79.8
Citric Acid) Citric Acid0.2


Fenarimol 12.5 (control)

No treatment
약효시험 약해시험
Drug Test Drug Test

의뢰기관

(주)황샘바이오

Client

Hwang Saem Bio Co., Ltd.
희석배수 처리시기 기준량 배량
및 사용량 및 방법

1,000배 발생기 1회 1,000배 500배
경엽처리 (6월14일)(6월14일) (6월14일)

3,000배


Dilution rate treatment time
And usage and methods

1,000 times 500 times of generators 1,000 times
Foliage Treatment (June 14) (June 14) (June 14)

3,000 times


사. 약제살포 전후 기상상황four. Weather situation before and after drug spraying

약효에 영향을 미칠만한 특별한 기상상황은 없었음.There were no special weather conditions that could affect the efficacy.

3. 조사방법3. Survey Method

구분 조사항목 조사횟수 조사일자 조사방법Classification Survey Items Survey Frequency Survey Date Survey Method 약효시험 발병도 2회 6월14일, 이병엽을 병반면적율로 조사
24일
약해시험 약해유무 3회 6월17일,
19일, 21일 외관상 약해유무 달관조사(0-5)
Two outbreaks of medicinal efficacy test
24 days
Test for weakness 3 times of weakness June 17,
Observation of the apparent weakness on the 19th and 21st (0-5)

4. 시험성적4. Test score

가. 약효시험end. Drug test

- 딸기 흰가루병에 대한 약제방제 효과(약제처리 7일 후)-Effect of pharmaceutical control on strawberry powdery mildew (7 days after pharmaceutical treatment)

처리전
시험약제 발병도
Before treatment
Test drug incidence
발병도            Onset
유의차-5% 방제가
(DMRT) (%)

Significant difference-5% control
(DMRT) (%)
1반복 2반복 3반복 평균1 Repeat 2 Repeat 3 Repeat Average 제독되지 않은 유황
(황+유용미생물+ 47.9 6.8 6.2 7.3 6.8 a 85.9
구연산)
훼나리 유제(대조) 45.8 3.7 3.1 4.8 3.9 a 91.6
무처리 46.5 47.5 51.4 48.6 49.2 b -
Un-Admired Sulfur
(Sulfur + useful microorganisms + 47.9 6.8 6.2 7.3 6.8 a 85.9
Citric acid)
Fenix emulsion (control) 45.8 3.7 3.1 4.8 3.9 a 91.6
Untreated 46.5 47.5 51.4 48.6 49.2 b-

나. 약해시험I. Weakness test

-약제처리 후 3, 5, 7일 후 조사3, 5, 7 days after drug treatment

시험약제 시험작물
(품종)
Test drug
(kind)
약해정도(0-5)      Weak degree (0-5) 비 고
Remarks
기준량 배량Baseline 제독되지 않은 유황 딸기
(황+유용미생물+구연산) (매향)
Uncontaminated Sulfur Strawberry
(Sulfur + useful microorganism + citric acid)
0 0    0 0 약해없음No weakness

5. 결과 요약5. Summary of Results

가. 약효end. Medicinal effect

딸기 흰가루병에 대한 시험약제인 제독되지 않은 유황(황+유용미생물+구연산)은 약제처리 7일 후 85.9%의 방제효과를 보였음.Unadulterated sulfur (sulfur + useful microorganism + citric acid), a test drug for strawberry powdery mildew, showed 85.9% control after 7 days of treatment.

나. 약해I. Weak

상기 시험약제의 기준량 및 배량을 각각 경엽 처리한 후 3회에 걸쳐 약해 유무를 달관 조사한 결과 약제처리에서 약해 증상이 관찰되지 않았음.Three doses of the test drug and the standard dose of the test drug were treated with leaves, respectively, and the results of the dilemma were examined.

6. 시험담당자 의견6. Examiner Comments

시험약제가 비교적 높은 방제효과를 보여 딸기 흰가루병에 대한 방제약제로 실용성이 있다고 판단됨.The test drug showed a relatively high control effect, which is considered to be practical as a control agent for strawberry powdery mildew.

상기와 같이 본원의 제독되지 않은 유황과 유용미생물군(EM) 및 구연산의 액상혼합액이 살포된 고추는 진딧물의 경우 3일 후 76.0%, 7일 후 82.9%의 방제효과가 나타났으며, 딸기 흰가루병의 경우 7일 후 85.9%의 방제효과가 나타나는 것으로 관찰되었다.
As described above, red pepper sprayed with the liquid mixture of sulfur and the useful microorganism group (EM) and citric acid of the present application showed control effects of 76.0% after 3 days and 82.9% after 7 days for aphids, and strawberry powdery mildew. In case of, 85.9% of control effect was observed after 7 days.

Claims (2)

유용미생물군(EM : Effective Micro organism)과 제독되지 않은 유황분말을 1 : 1 내지 20 : 1의 부피비율로 혼합한 액상 혼합액을 아크릴관이나 스텐레스 보호통 안에 60℃정도의 기름이나 물을 채우고 전기가열기를 넣어 간접 가열하여 35℃ ~ 60℃ 온도가 유지된 상태에서 24시간 방치하는 단계; 이 후 7일 ~ 14일간 매일 1 ~ 5회씩 회당 1 ? 20분간 제독되지 않은 유황과 유용미생물군(EM)의 액상혼합액을 나무막대기로 충분히 교반하는 단계와; 상기 액상혼합액 99.8중량%에 구연산 0.2중량% 혼합하는 단계로 이루어진 것에 특징이 있는 방제 방역 및 작물 생육용으로 이용하기 위하여 제독되지 않은 유황과 유용미생물군을 액상혼합하는 방법.
Fill the liquid mixture with the effective micro organism (EM) and the non-toxic sulfur powder in a volume ratio of 1: 1 to 20: 1 and fill it with oil or water at around 60 ℃ in an acrylic tube or stainless steel protective container. Indirect heating by placing hot air and leaving it at a temperature of 35 ° C. to 60 ° C. for 24 hours; After that, 1 to 5 times per day for 7 to 14 days. Sufficiently stirring the liquid mixture of sulfur and non-toxic microorganism group (EM) for 20 minutes with a wooden stick; 9. The method for liquid-mixing sulfur and the useful microorganisms, which are not detoxified for use in controlling and controlling crop growth, characterized in that the mixture of 99.8% by weight of citric acid and 0.2% by weight of the liquid mixture.
상기 청구항 1의 방제 방역 및 작물 생육용으로 이용하기 위하여 제독되지 않은 유황과 유용미생물군을 액상혼합하는 방법에 의해 조성된 조성물.
Composition prepared by a method of liquid-mixing sulfur and non-detoxified sulfur and the useful microorganism group for use in the control and prevention of crop growth of claim 1.
KR1020110042295A 2010-09-27 2011-05-04 A liquified mixing method of sulfur and em(effective micro organism) for preventing epidemic and exterminating of insect pests, and thereof a composite Pending KR20120031862A (en)

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KR101298676B1 (en) 2012-10-26 2013-08-26 (주)황샘바이오 Method for solid culturing sulfur and em(effective micro organism) and thereof a composite
KR101365273B1 (en) * 2013-10-24 2014-02-19 문성구 Fermentation fertilizer
KR101579969B1 (en) * 2013-11-21 2015-12-23 정해수 Method of manufacturing low toxic fermented sulfur disinfectant
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KR20090005729A (en) * 2007-07-10 2009-01-14 유희민 Animal feed and manufacturing method for producing functional and non-antibiotic livestock products using natural plant ingredients and useful microorganisms
KR100981780B1 (en) 2010-04-29 2010-09-10 (주)황샘 A liquefied making method for eliminated sulfur with em and thereof liquefied sulfur

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KR101334157B1 (en) * 2013-03-12 2013-11-29 농업회사법인 주식회사 엘바이오텍 Surfur removing poison produced using effective microorganism and uses thereof
KR20230028936A (en) * 2021-08-23 2023-03-03 주식회사 한국인삼공사 Control composition for bryophytes

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