KR20120005195A - Hair loss prevention and hair growth promoting composition comprising ginseng fruit extract - Google Patents

Abstract

 The present invention relates to a hair loss prevention and hair growth promoting composition comprising a ginseng fruit extract as an active ingredient. The ginseng fruit extract has no stimulation to the skin, and has an effect of providing a composition for preventing hair loss and promoting hair growth through hair growth promotion, hair loss prevention and treatment improvement due to excellent skin cell regeneration effect.

Description

Hair loss prevention and hair growth promoting composition comprising ginseng fruit extract {COMPOSITION INCLUDING GINSENG BERRY EXTRACT FOR PREVENTING HAIR LOSS AND PROMOTING HAIR GROWTH}

The present invention relates to a hair loss prevention and hair growth promoting composition comprising a ginseng fruit extract as an active ingredient.

In recent years, both domestically and globally, the age at which hair loss begins has decreased. In addition, as the human lifespan is extended, more and more people are suffering from hair loss during their lifetime. In line with this trend, interest in hair loss is increasing.

Known causes of hair loss include excessive secretion of the testosterone, a testosterone, decreased blood flow to hair follicles, genetic causes of hair follicle traits, side effects from drugs, overgrowth of bacteria and sebum secretion. Scalp abnormalities, age, and stress. However, the exact cause of hair loss is not yet clearly identified.

Human hair is about 1.3 million or more hair, 100,000 or more hair. These hairs go through three stages: anagen, catagen, and telogen, with each hair working in an independent cycle.

Hair loss refers to a condition in which hair or hair is less or less than normal due to some cause, and is classified into general hair loss phenomenon (telogen effluvium) in which hair falls out of a resting state and anagen effluvium, which is abnormal hair loss. can do. The latter is generally called hair loss.

Alopecia (or alopecia disorders) may include alopecia areata, hereditary androgen alopecia, telogen effluvium, traumatic, tritoti lomania, and pressure, depending on the form, symptoms, or causes Alopecia is classified as growing alopecia, nasal stiffness, alopecia syphlltiac, seborrheic alopecia (Alopecia seborrhecia), symptomatic, scarring, congenital alopecia.

 Hair loss treatment methods include hair follicle transplantation and drug treatment. Surgical methods are not simple and costly, and drug treatments have problems such as drug side effects and prolonged treatment period.

In addition, there are cases of using female hormones, but their effectiveness is not only excellent, but due to skin inflammation, side effects caused by hormone administration, etc. are currently discontinued.

As the type of hair regrowth, preparations based on Minoxidil, Trichosaccharide from Crinos, Italy, etc. are used, but there are no obvious effects and side effects. have.

In addition, Merck said that finasteride, which inhibits the activity of 5α-reductase, an enzyme that acts on male hormone testosterone metabolism in hair follicles, is effective for hair loss, it must be administered continuously. There is a problem that can cause depression.

In addition, crescina and rogaine are used only if the hair roots are alive in normal scalp before the hair loss disease is fully developed. As described above, various types of hair regrowth are generally intended to help hair growth for the purpose of promoting blood pressure and nourishing hair roots, but these are also toxic and side effects are severe and the effect is also insufficient.

Therefore, the development and research on functional products such as cosmetic products, shampoos, hair lotions, hair creams, hair gels, etc. aiming at preventing hair loss or promoting hair growth using natural materials are actively underway in Korea.

Among the ginsengs that are spotlighted as natural products, Panax ginseng is listed as a commodity in new agricultural plants, so it is less toxic and safe, sweet and slightly warm. It is the representative special product of Korea.

Physiologically active substances of Korean ginseng include 30 kinds of ginseng saponins including ginsenoside Rb ₂ with antidiabetic effect, polyacetylene with anticancer effect, phenolic compounds with antioxidant action, and ginseng protein with radiation defense action. And acidic polysaccharides having an immunomodulatory effect.

Ginseng saponin, known as the main pharmacological component of Korean ginseng, is called ginsenoside. Korean ginseng contains about 30 kinds of ginseng saponins, much more than 14 kinds of Panax quinquefolium and 15 kinds of Panax notoginseng .

Ginsenosides are largely divided into the protopanaxadiol group and the protopanaxatriol group. The main component of the ProtoPanaxadiol group is ginsenoside Rb ₁ , which represents the central nervous system inhibitory activity, and the main component of the ProtoPanaxatriol group is the ginsenoside Rg ₁ , which represents central nervous system excitability. They are known to be the major contributors to the activity of adaptogen (adaptogen) of Korean ginseng.

Studies on the prevention of hair loss or promoting hair growth of ginseng have been reported to have a hair conditioning effect of hydrolyzed ginseng saponin quaternary ammonium compounds (Kim et al . Int J Cosmet Sci . , 11 (5), 203-220, 1989) Red ginseng and ginsenosides Rb ₁ and Rg ₃ have been reported for hair growth promoting activity (Matsuda et al ., Phytother Res. 17 (7), 797-800 , 2003).

However, it is not known that ginseng fruit (pulp) active ingredient can be used as a composition for preventing hair loss or promoting hair growth.

Thus, while the present inventors are studying a functional product using ginseng fruit, when the ginseng fruit is extracted with a solvent, a high content of ginsenoside Re is obtained, and thus the composition obtained is effective in preventing hair loss and promoting hair growth. Thus, the present invention has been completed.

It is an object of the present invention to provide a composition for preventing hair loss and promoting hair growth, including ginseng fruit extract which is a safe natural product compared to synthetic chemicals.

Another object of the present invention is to provide a pharmaceutical composition and cosmetic composition comprising a ginseng fruit extract as an active ingredient.

In order to achieve the above object, the present invention provides a composition for preventing hair loss and promoting hair growth comprising ginseng fruit extract as an active ingredient.

The ginseng fruit extract may include 1 to 70% by weight of ginsenosides Re based on the total ginsenoside weight in the ginseng fruit extract.

The ginseng fruit extract may be an extract extracted from one or more selected from the group consisting of ginseng fruit, ginseng flower, ginseng stem and ginseng leaf.

Method for preparing the extract may be used in the conventional extraction methods such as ultrasonic extraction, filtration, hot water extraction and reflux extraction method.

Specifically, the ginseng fruit extract refers to a plant of the genus Panax such as Panax ginseng , Panax quinquefolium , Panax notoginseng , Panax japonicum or Panax vietnamensis . The above-mentioned herbal medicine, for example, ginseng fruit (pulp), ginseng (flower bud), ginseng stem, extracts of ginseng leaves, and may be used in the case of processing them.

The processing at this time may be extraction by a known method as necessary. Processed ginseng extract is extracted with water, lower alcohol (methanol, ethanol, etc.), lower ketone (acetone, methyl ethyl ketone, etc.), supercritical fluid, or a mixed solvent thereof, and then concentrated or concentrated liquid. The dried ginseng extract, ie, ginseng, may be prepared in the form of a soft or dry extract by removing the solvent by drying.

The extractant may be selected from the group consisting of water, C ₁ -C ₄ alcohols, n-hexane, ethyl acetate, and mixed solvents thereof. In addition, the extraction solvent is preferably used an aqueous ethanol or methanol aqueous solution having an ethanol concentration of 70 to 95%.

When the ethanol concentration of the ethanol aqueous solution is less than 70%, less active ingredient having a human physiological activity in the ginseng fruit can be extracted, and when the ethanol concentration is greater than 95%, causing side effects to the skin due to the stimulation of alcohol, ethanol aqueous solution of the above range Preference is given to using. In addition, the methanol aqueous solution may be used having a methanol concentration of 100%.

Extraction using the ethanol aqueous solution and the like is preferably extracted by heating at 40 to 120 ℃ for 0.5 to 4 hours.

If the heating extraction temperature is less than 40 ℃ extraction content of the active ingredient, such as ginsenoside Re in ginseng fruit may be less, and when the extraction temperature is higher than 120 ℃ ginsenoside Re extraction efficiency does not increase any more economical efficiency In consideration of this, it is preferable to extract by heating within the above range.

If the heating time is less than 0.5 hours, the extraction content of the active ingredient, such as ginsenoside Re in the ginseng fruit may be reduced, and if more than 4 hours, the extraction efficiency of ginsenoside Re does not increase any more economically. In consideration, it is preferable to heat-extract within the said range.

And when the extraction using an ethanol aqueous solution or the like is performed at room temperature, it may be performed for 5 to 10 days, preferably about 7 days. It may also be carried out under shading and / or warming (eg warming) conditions as needed.

In particular, by adding 5 to 15 times the amount of alcohol to the ginseng fruit (pulp), and extracted by heating at 40 to 120 ℃ for 0.5 to 4 hours, an extract containing 1 to 70% by weight of ginsenoside Re can be obtained. In addition, 5-15 times the amount of distilled water is added to the ginseng fruit (pulp) and extracted by heating at 40 to 120 ° C. for 0.5 to 4 hours to obtain an extract containing 1 to 70% by weight of ginsenoside Re.

The extraction process may be performed once or plural times, preferably about three times. The mixed extract may be obtained in a liquid, semi-solid or powder form by a conventional method, for example, by concentrating under reduced pressure or drying under reduced pressure at 20 to 40 ° C. as necessary.

When using such a solvent extraction method there is an advantage that can extract a lot of active ingredients compared to the boiling water extraction method boiled with water. In addition, in order to increase the extraction content of the active ingredient in ginseng fruit, a chromatography fractionation process may be further performed.

The ginseng fruit extract has an effect of promoting the growth of human dermal papilla cells. In addition, due to excellent skin cell regeneration effect can be used to promote hair growth, prevent hair loss and treatment.

The composition of the present invention can be used as a pharmaceutical composition having a formulation selected from the group consisting of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, external preparations and sterile injectable solutions.

More specifically, the composition of the present invention may be in the form of a pharmaceutical composition comprising the ginseng fruit extract and a pharmaceutically acceptable carrier. The pharmaceutical composition may be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, oral formulations, external preparations and sterile injectable solutions according to conventional methods.

The pharmaceutically acceptable carrier is lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline Cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil and the like.

Also included are diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, surfactants, and the like. Oral solid preparations include tablets, pills, powders, granules, capsules and the like, and such solid preparations include at least one excipient such as starch, calcium carbonate, sucrose or lactose. ), Gelatin, and the like, and may include a lubricant such as magnesium stearate, talc, and the like.

Oral liquid preparations include suspensions, solvents, emulsions, syrups, and the like, and may include water, diluents such as liquid paraffin, wetting agents, sweeteners, fragrances, preservatives, and the like. Parenteral preparations include sterile aqueous solutions, non-aqueous solvents, suspending agents, emulsions, and lyophilized preparations. Non-aqueous solvents and suspending agents include propylene glycol, polyethylene glycol, and vegetable oils such as olive oil and ethyl oleate. Injectable esters such as the like and the like.

Meanwhile, the dosage of the ginseng fruit extract contained in the pharmaceutical composition varies depending on the condition and weight of the patient, the degree of the disease, the drug form, the route of administration and the duration, and may be appropriately selected in some cases. For example, the ginseng fruit extract may be administered at a dose of 0.0001 to 100 mg / kg, preferably 0.01 to 100 mg / kg, and the administration may be administered once or several times a day.

In addition, the pharmaceutical composition may comprise 0.001 to 50% by weight of the ginseng fruit extract based on the total weight of the composition. The pharmaceutical compositions can be administered to mammals, such as humans, by various routes, for example by oral, intravenous, intramuscular, or subcutaneous injection.

And the composition of the present invention It may be in the form of a functional cosmetic composition comprising the ginseng fruit extract as an active ingredient. For example, it may be used as a cosmetic composition having a formulation selected from the group consisting of softening cream, converging cream, nourishing cream, nourishing cream, massage cream, essence, hair dye, shampoo and rinse. And the composition of the present invention can be used diluted with a conventional cleansing liquid, astringent liquid and moisturizing liquid, and may include conventional auxiliaries such as stabilizers, solubilizers, vitamins, pigments, and fragrances commonly used in the cosmetic composition field. Can be.

The pharmaceutical and cosmetic compositions of the present invention are not limited to the above-mentioned forms, and may be prepared in various forms as long as it is used for hair loss prevention and hair growth. That is, the ginseng fruit extract according to the present invention can be utilized in various functional products.

The composition comprising the ginseng extract according to the present invention as an active ingredient, there is no irritation to the skin, and has the effect of preventing hair loss and promoting hair growth.

1 is a HPLC chromatogram showing the results of ginsenoside analysis of Korean ginseng seed extract (KGS) (a: Rg1, b: Re, c: Rb1, d: Rc, e: Rb2, f: Rd).
Figure 2 is an HPLC chromatogram showing the results of ginsenoside analysis of Korean ginseng fruit extract (KGB).
3 is a graph showing the effect of ginseng fruit extract on the growth of human hair dermal papilla cells (HHDPC)
Figure 4 is a photograph showing the effect of ginseng fruit extract on the hair growth of C57BL / 6 mice.
5 is a histological cross-sectional view of hair follicles showing the effect of ginseng fruit extract on the hair growth of C57BL / 6 mice.
Figure 6 is a graph showing the mass of the hair of C57BL / 6 mice to which ginseng fruit extract is applied.

Hereinafter, the structure of the present invention will be described in more detail with reference to examples, but the scope of the present invention is not limited to the following examples.

Example  1: Preparation of Ginseng Fruit Extract (95% Ethanol Extract)

100 g of ginseng fruit (cultivated in Jeonbuk Gimje) was added to 10 times of 95% ethyl alcohol and extracted with two reflux cooling at 80 ° C. for 2 hours, and then concentrated under reduced pressure and lyophilized to obtain a reddish brown ginseng fruit extract.

Example  2: Preparation of Ginseng Fruit Extract (70% Ethanol Extract)

100 g of ginseng fruit (cultivated in Jeonbuk Gimje) was added to 10% of 70% ethyl alcohol, extracted with two reflux cooling at 70 ° C. for 2 hours, concentrated under reduced pressure, and lyophilized to obtain a reddish brown ginseng fruit extract.

Example 3: Preparation of Ginseng Fruit Extract (100% Methanol Extract)

A mixture of 70 g of ginseng fruit and 30 g of ginseng (both grown in Jeonbuk Gimje) was extracted with 10 times of 100% methyl alcohol with two reflux cooling at 80 ° C. for 2 hours, concentrated under reduced pressure and freeze-dried to reddish brown. Ginseng fruit extract was obtained.

Example 4: Preparation of Ginseng Fruit Extract (Hot Water Extract)

100 g of ginseng (cultivated in Jeonbuk Gimje) was added to 10 times distilled water and solvent extracted while cooling under reflux twice at 100 ° C. for 2 hours. The extract was then concentrated under reduced pressure and lyophilized to give a reddish brown ginseng fruit extract.

Experimental Example 1: Ginsenoside analysis of ginseng fruit extract

Ginsenoside analysis of the ginseng fruit extract obtained in Example 1 was performed by HPLC. Ginsenoside analysis was performed with reference to known methods (KO SK et al., Food Sci & Biotechnol 14: 509-513, 2005; KO SK et al., Korean J. Food Sci. Technol . 35: 536-539, 2005; Lee CR et al., Korean J. Food Sci . Technol . 36: 847-850, 2004). Pure ginsenoside standard (99% purity) was purchased from Chromadex (St. Santa Ana, CA, USA.). The HPLC instrument was a Waters 1525 binary HPLC system (Waters, MA, USA) and the column was Gemini 5μC18110A (Phenomenex, 4.6 × 250 mm, CA, USA). The mobile phase was a mixture of acetonitrile (HPLCGrade, Sigma-Aldrich Chem Co., MO, USA) and distilled water (HPLC grade, JT Baker, NJ, USA). Acetonitrile concentrations increased continuously from 17% to 40% (40 min), 40% to 60% (90 min), 60% to 80% (95 min, stay for 105 min), and finally at 80% Adjusted to 17% (115 min, stay for 10 min). The operating temperature was room temperature and the flow rate was 1.0 mL / min. The elution profile of the chromatogram was obtained using a UV / VIS detector (Waters 2487 dual λabsorbance detector, MA, USA) at 203 nm.

In addition to the ginseng fruit extract (KGB) obtained in Example 1, Korean ginseng seed extract (KGS, purchased from Jeonbuk Gimje), Korean ginseng root extract (KGR, purchased from Jeonbuk Gimje) and US ginseng seed extract (AGS, Wausau ( WI, USA)) ginsenoside content was also analyzed using the above-mentioned HPLC analysis method, the ginsenoside analysis results are shown in Table 1, Figures 1 and 2. The extraction method for obtaining Korean ginseng seed extract, Korean ginseng root extract and US ginseng seed extract was used in the method described in Example 1. As shown in Figure 1, 2 and Table 1, it can be seen that the ginseng extract according to the present invention has a high content of Re.

As can be seen through Table 1, the ginseng fruit extract according to the present invention can be seen that the content of Re in ginsenosides is high.

Experimental Example  2: ginseng fruit extract is human Breast milk  cell( HHDPC ) Assessment of impact on growth

In order to investigate the effect of the ginseng fruit extracts obtained in Examples 1 to 4 on cell growth of human dermal papilla cells, human hair dermal papilla cells (HHDPC) (Sciencell Co., Cat.No. 2400) were used. Evaluated.

In Mesenchymal Stem Cell (MSC) medium, a dedicated medium containing 10% fetal calf serum (FBS, Cambrex, USA), make the dermal papilla cells 0.25 x 10 ⁴ cells / ml and inoculate 96-well plates. For hours, incubated under 37 ° C., 5% CO ₂ wet conditions.

The culture solution of the ginseng fruit extract obtained in Examples 1 to 4 at concentrations of 0.4, 20, 100, and 500 ug / ml, respectively, was cultured for 24, 48 and 72 hours, and then 5 mg / ml of MTT (3- 20 [mu] l of (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide) reagent was added to the culture solution and incubated for 4 hours under 3 ° C and 5% CO ₂ wet conditions. As a control, a culture of cells not treated with ginseng fruit extract was used.

In culture, purple crystals form on living cells. After incubation for 4 hours, the medium was removed, 100 μl of DMSO was added, the crystals were dissolved in a shaking incubator for 10 minutes, and the absorbance was measured at 540 nm to measure the extent of cell growth. The result is shown in FIG. 3, it can be seen that ginseng fruit extract promotes the growth of dermal papilla cells.

When the ginseng fruit extract was treated with 0.4-500 ug / ml of Human Hair Dermal Papilla Cell, there was no significant cytotoxicity at all concentrations. The ginseng fruit extract was found to promote cell growth from 121% to 135% of human dermal papilla cells in a concentration-dependent manner.

Therefore, based on the growth of the dermal papilla cells of the ginseng berry extract, the ginseng fruit extract is thought to exhibit hair growth prevention and hair growth promoting effect.

Experimental Example 3: Hair Growth Effect in C57BL / 6 Mice by Ginseng Fruit Extract

To measure the hair growth effect in C57BL / 6 mice by ginseng fruit extract, After 7 weeks of age C57BL / 6 mice were anesthetized, the back hair was completely removed from the hair follicle cells using waxing resin. Then, the mice with the back hair removed were divided into four groups of 10 mice each. As shown in Figure 4, the hair on the back portion was removed with an electric hair remover and no treatment (normal) was prepared separately. Group A was applied as a control, 70% ethanol once daily and observed changes for a total of 21 days. Group B applied minoxidil once daily for 21 days. Group C and D were dissolved in 50% ethanol in the ginseng fruit extract obtained in Example 1 and applied to the skin such as mice once daily for 21 days at doses of 1 mg / ml and 10 mg / ml, respectively, for a total of 21 days Observed. After these treatments, changes in the mice were observed until day 21 and are shown in FIG. 4.

In addition, in order to observe changes in hair follicle, skin thickness, and tissue in the skin, one of the mice in each group was killed on the 14th and 21st days, and then the tissue of the hair growth site of the dorsal skin was removed. Extracted and fixed with 4% formaldehyde solution. After fixation, the cells were embedded with paraffin through washing and dehydration, and 7 μm sections were prepared and stained with H & E (Hematoxylin & Eosin). The visual and histological changes are shown in FIG. 5. .

4, the back of the mouse treated with ginseng fruit extract (C and D group) compared to the group not treated with the ginseng fruit extract can be confirmed that the skin color is black in a short time and the hair grew quickly.

In Figure 5 through the actual skin tissue, ginseng fruit extract treatment group (C and D group) in the low concentration (100 ug / horse) of the number of hair follicles compared to the control group 300 days compared to the negative control group 14 days, high concentration (10 mg / Horse) increased by 188%, and on day 21, it increased by 123% compared to the negative control group and 108% (16/26) at higher concentrations (10 mg / horse). In this case, the positive control group showed an increase rate of 325% and 123%, respectively. In particular, it was observed that the hair follicles were promoted by morphological changes of hair follicles in the high concentration group rather than the number of hair follicles.

Figure 6, after taking ginseng fruit extract 21 days after taking the skin tissue of the dorsal 1 cm x 3 cm area of the group C mice, the weight of the newly grown hair is measured, and compared with the control (A and B) It is shown. 6, in the case of the group treated with ginseng fruit extract, it can be confirmed that the hair growth effect is excellent compared to the negative control.

Therefore, ginseng fruit extract according to the present invention is composed of a safe substance without skin irritation, it can be seen that it can prevent hair loss and promote hair growth.

Preparation Example 1: Preparation of external preparation for skin containing ginseng fruit extract

1-1: Massage Cream with Ginseng Fruit Extract

The preparation example of the massage cream containing the ginseng fruit extract of the present invention is as shown in Table 2 below. The aqueous phase purified water, triethanolamine and glycol (1,3-Propanediol) are heated and dissolved at 70 ° C., and the oily fatty acid, oily component, emulsifier and preservative are heated to 70 ° C. and dissolved to add the emulsified product. After emulsification is completed, the solution is cooled to 45 ° C., the ginseng fruit extract and flavor are added and dispersed, and then cooled to 30 ° C.

1-2: Tablets containing Ginseng Fruit Extract

Preparation of tablets containing ginseng fruit extract is shown in Table 3 below.

Simple modifications or changes of the present invention can be easily carried out by those skilled in the art, and all such modifications or changes can be seen to be included in the scope of the present invention.

Claims (8)

Contains ginseng fruit extract as an active ingredient, hair loss prevention and hair growth promoting composition.
The composition for preventing hair loss and promoting hair growth according to claim 1, wherein the ginseng fruit extract comprises 1 to 70% by weight of ginsenoside Re based on the total ginsenoside weight in the ginseng fruit extract.
According to claim 1, The ginseng fruit extract is characterized in that the extract is extracted from one or more selected from the group consisting of ginseng fruit, ginseng, ginseng stem and ginseng leaves, hair loss prevention and hair growth promoting composition.
According to claim 1, wherein the ginseng fruit extract, characterized in that it has a growth promoting activity of human dermal papilla cells, hair loss prevention and hair growth promoting composition.
The method of claim 1, wherein the extract is any solvent selected from the group consisting of water, C ₁ -C ₄ alcohol, n-hexane, ethyl acetate and a mixed solvent thereof, 0.5 to 4 at 40 to 120 ℃ A composition for preventing hair loss and promoting hair growth, which is obtained by heating for a time.
The pharmaceutical composition of claim 1, wherein the composition is formulated in one form selected from the group consisting of powders, granules, tablets, capsules, suspensions, emulsions, syrups, oral formulations of aerosols, external preparations and sterile injectable solutions. A composition for preventing hair loss and promoting hair growth, characterized in that the composition.
The composition for preventing hair loss and promoting hair growth according to claim 1, wherein the composition is a cosmetic composition having a formulation selected from the group consisting of massage creams, hair essences, hair dyes, shampoos and rinses.
According to claim 1, The ginseng fruit extract is characterized in that it comprises 0.001 to 50% by weight based on the total weight of the composition, hair loss prevention and hair growth promoting composition.

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