KR20120000134A - Cosmetic composition containing volcanic stone clusters - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a volcanic stone cluster, and more particularly, contains a volcanic stone cluster as an active ingredient, inhibits sebum secretion excessively secreted from the skin, removes free radicals and promotes the synthesis of collagen. The present invention relates to a cosmetic composition for sebum control, pore reduction and skin trouble suppression, which has an excellent effect of reducing the expression of skin inflammatory factors.

Description

Cosmetic composition containing volcanic stone clusters {Cosmetic composition containing scoria}

The present invention relates to a cosmetic composition containing a volcanic stone cluster, and more particularly, contains a volcanic stone cluster as an active ingredient, inhibits sebum secretion excessively secreted from the skin, removes free radicals and promotes the synthesis of collagen. The present invention relates to a cosmetic composition for sebum control, pore reduction and skin trouble suppression, which has an excellent effect of reducing the expression of skin inflammatory factors.

In general, sebum in the skin including the scalp and face is responsible for maintaining the skin's moisturizing or microorganism invasion, but after puberty will promote acne. In particular, women in their 30s and older may cause problems such as skin swelling, makeup lifting and pore enlargement due to excessive secretion of sebum. There are various causes for the excessive secretion of sebum, but it is known that the sebaceous gland secretion is caused by the activation of the cells of sebaceous gland by the amount of dihydrotestosterone, one of the hormones involved in promoting sebum secretion in the activity of sebaceous glands. have.

5α-Reductase is present in testosterone-reactive tissues such as sebaceous glands, hair follicles, prostate, and epididymis. As an enzyme involved, its conversion requires NDPH (NADPH). Testosterone is involved in skeletal muscle growth and spermatogenesis, and dihydrotestosterone is involved in tissues in acne, sebum and prostatic hyperplasia (Diane et al; J. Invest Dermatol. 775-778, 1995. Bruchovsky, N et al; JBC 243, 2112-2121, 1968). In addition, in the sebaceous glands of the skin, testosterone is converted into dihydrotestosterone by 5α-reductase, binds to a receptor protein in the cytoplasm, enters the nucleus, activates sebaceous gland cells, and promotes differentiation of sebum in sebaceous glands. (Diane et al; J. Invest Dermatol. 105; 209-214).

Active oxygen species act as a causative agent of skin aging in particular. The skin continues to undergo photochemical reactions that produce free radicals when exposed to ultraviolet light. These reactive oxygen species lead to skin cell and tissue damage. They break the antioxidant defenses made up of antioxidant and non-enzymatic antioxidants, shifting the oxidant / antioxidant balance towards oxidation. As a result, continued oxidative stress leads to lipid peroxidation, protein oxidation, activation of proteolytic enzymes that destroy skin components, chain cleavage and abnormal cross-linking of the elastic fibers collagen elastin, cleavage of hyaluronic acid chains, promotion of melanogenesis, DNA Causing damage to biological components such as oxidation. Various aging phenomena appear due to the damage of the biological components. In addition to well-known aging phenomena such as wrinkle formation and reduced elasticity, pores widening also appear as aging phenomena. Many studies have been conducted on aging phenomena such as wrinkle reduction and elasticity reduction, but not much about pores. But the most important cause of pore widening is oxidative stress.

Looking at the structural changes of the skin due to oxidative stress, the thickness of the skin's components epidermis, dermis and subcutaneous tissue becomes thin. In addition, the extracelluar matrix (ECM) component of dermal tissue responsible for skin elasticity and tension is changed. ECM consists of two main components. One is elastic fiber, an elastic fiber that makes up about 2-4% of the total ECM, and the other is collagen, which makes up about 70-80% of the total ECM. Collagen breaks down under the influence of oxidative stress, resulting in a decrease in collagen in the skin. As it ages, the extracellular matrix around the pores decreases, resulting in wider pores.

Therefore, the best way to prevent the pores from widening is to suppress oxidative stress, and the best way to reduce the enlarged pores is to increase the production of collagen around the pores.

Many studies have been conducted on antioxidants that are not directly related to pore widening but can prevent oxidative stress, and many antioxidants have been developed and used, especially vitamin C and vitamin E. to be. Vitamin C is a water soluble antioxidant and vitamin E is a fat soluble antioxidant. However, these antioxidants have strong antioxidant effects, but there are many limitations in using them because they are easily oxidatively degraded when exposed to air due to instability. Another conceivable antioxidant is polyphenols. But polyphenols also have drawbacks that indicate instability.

In urban areas and industrial complexes, yellow dust or external contaminants flying through automobile emissions, industrial zones in eastern China, including fine heavy metal powder, can contaminate people's skin and cause skin aging and skin problems. This is mainly due to heavy metals and fine dust in the pollutants.

The heavy metals refer to all metals having a specific gravity of 4 or more, such as mercury, cadmium, lead, and copper, and generally, when absorbed into a living body, they combine with a living body to form an organic complex that does not decompose well, so that the liver is not quickly discharged out of the body. It is a substance that is strongly accumulated in organs and bones, such as kidneys. Prolonged exposure to small amounts of heavy metals may cause symptoms such as paralysis, abnormal movements, ataxia, pigmentation and keratinization of the skin, atrophy of the toenails or hooves and hair, reproductive dysfunction, malformation, decreased growth, and reduced immune function. appear. In the skin, these harmful heavy metal fine dusts cause chemical reactions in the air to generate additional harmful substances such as nitrogen oxides (NO) and sulfur oxides (SO) to increase cytokines, which are inflammatory substances in the skin, and cause atopic or skin troubles. Can be.

Current methods of removing heavy metals in the body include injecting complex forming agents such as EDTA (ethylene diamine tetraacetate) and BAL (British Anti-Lewisite) to bind heavy metals accumulated in the body to release heavy metals out of the body or vitamin B1, Take vitamin C and vitamin E to prevent the absorption of heavy metals in the body and facilitate the discharge. However, there is no effective and easy way to remove heavy metals that accumulate on the skin surface. Fine dust can be removed to some extent by cleansing, but the finer it is, the stronger the adsorption power, so it is impossible to completely remove fine dust that has penetrated deep into the pores with normal cleansing. It is a necessary time.

Therefore, the inventors of the present invention while trying to solve the above problems and to find a natural product with a high effect of sebum control, pore reduction and skin trouble suppression, volcanic stone clusters confirmed that the sebum control, pore reduction and skin trouble suppression effect is excellent The present invention has been completed.

Therefore, it is an object of the present invention to provide a cosmetic composition which is excellent in sebum control, pore reduction and skin trouble suppressing effect by containing natural products safe to the human body.

In order to achieve the above object, the present invention provides a cosmetic composition for sebum control, pore reduction and skin trouble suppression containing a volcanic stone cluster (Scoria) as an active ingredient.

The cosmetic composition provided by the present invention contains a volcanic stone cluster to suppress sebum that is excessively secreted when applied to the skin, shrinks pores through the removal of free radicals and promotes collagen synthesis, and reduces the expression of inflammatory factors to improve skin The effect of suppressing trouble is excellent. In addition, by using a mixture of cedar extract, rapeseed flower extract, bamboo extract or green tea extract with volcanic stone clusters, it is possible to obtain more improved sebum control, pore reduction and skin trouble suppressing effect.

1 is a graph showing the effect of inhibiting the production of reactive oxygen species of keratinocytes by ultraviolet light of the volcanic stone cluster according to the present invention.

The present invention provides a cosmetic composition for sebum control, pore reduction and skin trouble suppression containing volcanic stone cluster (Scoria) as an active ingredient.

Hereinafter, the present invention will be described more specifically.

The volcanic stone cluster (Scoria) used as an active ingredient in the present invention is a kind of volcanic ash that exists only in Jeju Island in Korea, and is known to be produced when parasitic volcanoes (origin) are formed throughout Jeju, and the reserve in Jeju Island is about 20 billion m ³ It is estimated to be an almost infinite resource. Volcanic stone cluster is a natural material that can combat E. coli of 92 ~ 93% of far infrared ray, 97% of deodorization rate, and 99.9% of antibacterial fungus in its natural state, and it is not comparable to jade, ocher, elvan, germanium which are widely used in recent years. It is a natural material with the best features.

The volcanic stone cluster of the present invention can be obtained by a method conventional in the art, and the production method thereof is not particularly limited. For example, clusters collected in areas not contaminated with pesticides or the like may be obtained by pulverizing various particle sizes according to the use to remove impurities and dry heat sterilizing at a temperature of 150 ° C. or higher.

The cosmetic composition according to the present invention further comprises at least one selected from the group consisting of cedar (Cryptomeria japonica) extract, rape (Brassica camperstris (rapeseed) flower) extract, bamboo (Bambusa vulgaris) extract and green tea (Camellia sinensis leaf) extract It may contain.

Cedar (Cryptomeria japonica) used in the present invention grows well in valleys with an average annual temperature of 12-14 ° C. and rainfall of 3,000 mm and reaches 40 m in height and 1 to 2 m in diameter. The bark is reddish brown, split vertically, with dense branches and leaves, and becomes a cone-shaped tree. Leaves are curved needle-shaped, arranged in a spiral shape, and do not fall off when they dry. Hermaphrodite flowers bloom in March. The male flower is gathered at the end of a small branch, and the female flower is a ball-shaped one at the end of a short branch. Fruits are coniferous, woody, almost round, 2-3cm long. Fruit slices are thick, with sharp processes at the end. Seeds are 2-6 pieces at the bottom of the fruit pieces, long oval, with narrow wings around. Cedar wood has various variations depending on the color and splitting of the bark, tree type, the angle of the branches, the color and form and length of the leaves, the degree of baking, and the place of growth. There are horticultural varieties and afforestation varieties, and because of their long age, there are many varieties with various legends.

The rape flower (Brassica camperstris (rapeseed) flower) used in the present invention is a mustard and biennial herbaceous flowers bloom in March-April in turn and hang on the ends of branches. The height is 80 ~ 130cm and the surface is smooth and green. In the main stem, about 15 primary side branches come out, and 2 to 4 secondary side branches emerge from this branch. The leaves are lanceolate and have a dull tip. The leaves on the lower stem have long petioles and the leaf edges are deeply divided. The leaves on the upper stem do not have petioles, they surround the stem and their ends are thin. The leaves of Western species are thick and leathery (leather-like texture). The common species is light green and the leaves are non-leather. Stems usually have 30 to 50 leaves. Seeds contain 38-45% oil, which is edible oil containing 15-20% soluble nitrogen and 20% protein, which is consumed next to soybean oil and is used as salty feed or fertilizer. Flowers are wheat plants, and fatty oils separated from seeds are used as meat products, oil-based solvents, or machine lubricants.

Bamboo (Bambusa vulgaris) used in the present invention is a generic term for perennial plants belonging to the monocotyledonous rice plant (Paceae), the family Bambusoideae, sometimes referred to as only the tall king genus bamboo. Chinese characters are called bamboo. When bamboo was moved to the north, the name came with the southern sound of China. The southern treble of bamboo bamboo is tek, but the sound of the ending sound is weakened, so it is changed to large in Korea and divided into two syllables in Japan. It is distributed throughout the world but grows mainly in the tropics, especially in the Asian monsoon region. It is widely distributed in south central and Jeju islands in Korea. In the case of tall royal genus, it can grow up to 30m in height and 30cm in diameter. Stems are round, round, and hollow. The growth method is that the stem in the ground extends to the side and grows naturally by removing roots and sprouts from the node. The leaves are narrow and long. Likes wet land and grows fast. It also rarely blooms, but when it blooms, I bloom all at once in a bamboo field. The flower of bamboo is a kind of mutation that is not related to the propagation of bamboo. It is also known as a flowering disease or a natural highland. The flowering period varies from 3 years, 4 years, 30 years, 60 years, 120 years, and so on. Bamboo has a wide variety of uses, ranging from building materials, furniture, fishing rods, plant supports, to basketwork such as baskets, and is also planted for ornamental purposes. Young sprouts are cooked and eaten with herbs.

In addition, the green tea (Camellia sinensis leaf) used in the present invention inactivates the enzyme by treating the oxidase present in the tea leaves with heat or steam using the shoots or leaves of Camellia sininsis of Camellia family. It is made by. Green tea has not only been consumed as a symbol tea since BC, but as the pharmacological mechanism of various ingredients contained in green tea has recently been revealed, its value has been recognized. It contains a large amount of active ingredients such as polyphenols, caffeine, amino acids, and vitamin C. It has been found to be effective in hangover and nicotine detoxification, fatigue recovery, cardiovascular action, and skin care. Antioxidant, anticancer, blood cholesterol lowering, anti-aging, heavy metal detoxification, caries prevention and bad breath removal effect has been attracting much attention.

Cedar extract, rapeseed extract, bamboo extract and green tea extract of the present invention can be prepared by extraction by a method known in the art, the method is not particularly limited. Preferably, the extraction may be performed using an extraction solvent in which water and 1,3-butylene glycol are mixed in a weight ratio of 1: 1. At this time, the extraction temperature is preferably 40 ℃, it can be extracted for 6 to 24 hours. If the extraction temperature and the extraction time is out of the extraction efficiency may be lowered or a change of components may occur.

The cosmetic composition according to the present invention contains volcanic stone clusters, cedar extract, rape blossom extract, bamboo extract and green tea extract in an amount of 0.0001 to 80.0% by weight, more preferably 0.01 to 50.0% by weight, based on the total weight of the composition. If the content is less than 0.0001% by weight, the effects of sebum control, pore reduction and skin trouble suppression by the above-mentioned ingredients are insignificant, and when the content is more than 80.0% by weight, the increase of the effect is not large compared to the increase in content, which is rather inefficient.

To date, volcanic stone clusters, cedar, rapeseed, bamboo, and green tea extracts have been widely used for different purposes, but when used alone or in combination, sebum control, pore reduction, and skin trouble suppressing efficacy are not disclosed.

The cosmetic composition for sebum control, pore reduction and skin trouble suppression provided by the present invention contains volcanic stone clusters to suppress excessive secretion of sebum when applied to the skin, promote free radicals and collagen synthesis to shrink pores. In addition, the effect of suppressing skin problems is excellent by reducing the expression of inflammatory factors. In addition, in the case of using the cedar extract, rapeseed extract, bamboo extract, green tea extract or a mixture thereof with volcanic stone clusters, the synergy of these sebum control, pore reduction, trouble suppression effect is further improved. Therefore, the cosmetic composition of the present invention may be provided as a cosmetic composition for sebum control, pore reduction or skin trouble suppression.

Cosmetic compositions according to the invention may be formulated containing a cosmetically or dermatologically acceptable medium or base. These are all formulations suitable for topical application, for example emulsions, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes) obtained by dispersing an oil phase in solutions, gels, solids, pasty anhydrous products, aqueous phases and It may be provided in the form of a nonionic vesicle dispersant or in the form of a cream, skin, lotion, powder, ointment, spray, pack, or cone stick. It may also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant. These compositions may be prepared according to conventional methods in the art.

In addition, the cosmetic composition according to the present invention is a fatty substance, an organic solvent, a dissolving agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, water, an ionic type or Nonionic emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredients commonly used in cosmetics It may contain adjuvants conventionally used in the cosmetic or dermatology field. Such adjuvants are introduced in amounts generally used in the cosmetic or dermatological arts.

Hereinafter, the configuration and effects of the present invention will be described in more detail with reference to test examples and examples. However, these test examples and examples are provided only for the purpose of illustration in order to help the understanding of the present invention is not limited to the scope and scope of the present invention.

[Test Example 1] 5α-reductase activity inhibitory effect

Mature Sprogue-Dawley male rats (7-8 weeks old) were killed with diethyl ether, and the abdominal prostate was removed to remove connective tissue and buffered (0.32 M sucrose, 0.1 mM dithiothreitol, 20 mM). sodium acetate) was added to the finely chopped and suspended in a grinder. The suspension was centrifuged to obtain supernatant, partially purified 5α-reductase, and the activity inhibition experiment of this enzyme was performed.

Taking a portion of the supernatant obtained above was placed in a buffer containing 0.2M monobasic acid and 0.2M dibasic acid, is reacted with isotope substrate (testosterone) marked with ³ H measure the amount of dihydrotestosterone product It was. The reaction solution was prepared by adding 1 mM dithiothreitol, 40 mM sodium phosphate (pH 6.5), 50 μM NADPH, [1,2,6,7-3H] testosterone / testosterone (2.2X10 ⁹ mol), and enzyme suspension (0.8 mg). The volume was brought to 565 μl.

The sample was dissolved in 10% ethanol to 0.1%, and 5.65 μl of the above reaction solution was added so that the final concentration was 0.001%. The same volume of solvent was used as a control, and riboflavin was used as a positive control.

The reaction was started with the addition of the enzyme suspension and proceeded at 37 ° C. for 30 minutes, followed by extraction with 1 ml of ethyl acetate. 100 µl of ethyl acetate was extracted from silica plastic sheet kiesel gel 60 F 254 (Silica plastic sheet kieselgel). The developing solvent system on F254) was developed with acetate-cyclohexane (1: 1).

After drying the plastic sample in air, a bath system was used to measure the isotope amount. The dried plastic sheet and the x-ray film were put together in a bath cassette to allow isotopes of testosterone and dihydrotestosterone remaining on the film after one week. The quantification was measured. The results are shown in Table 1 below.

The volcanic stone cluster used in this experiment (trade name: Volcanic stone cluster, manufacturer: Bioland Co., Ltd.) used raw materials processed in powder form, and cedar extract, bamboo extract, green tea extract, and rape extract (manufacturer: Bioland Co., Ltd.) 100 g of the dry weight of each sample was placed in each flask, and extracted by chilling with 3 L of distilled water for 1 day, followed by filtration to remove debris.

sample T (dpm) DHT (dpm) % Conversion Inhibition rate (%) Volcanic Stone Cluster 8750 2873 22.4 53.3 Volcanic Stone Cluster + Cedar Extract (1: 1) 9150 2890 21.1 56.04 Volcanic Stone Cluster + Rape Blossom Extract (1: 1) 9320 2930 19.7 58.9 Volcanic Stone Cluster + Bamboo Extract (1: 1) 9280 2910 19.2 60.0 Volcanic Stone Cluster + Green Tea Extract (1: 1) 9420 2940 20.2 57.9 Volcanic Stone Cluster + Cedar Extract + Rape Flower Extract + Bamboo Extract + Green Tea Extract (1: 1: 1: 1: 1) 9830 2970 18.7 61.0 Control group 7430 5935 48.0 - Positive control group 7500 2530 29.7 38.1

(1) T: 3H Radio activity in the testosterone region

(2) DHT: 3H radioactivity in the dihydrotestosterone region

(3) Conversion rate: radioactivity / total radiation to the DHT region

(4) Inhibition rate: 100 * (control conversion-sample conversion) / control conversion

From the results of Table 1, the volcanic stone cluster of the present invention converts testosterone to dihydrotestosterone, binds to a receptor protein in the cytoplasm, enters the nucleus, activates sebaceous gland cells and promotes differentiation, thereby over secreting sebum in sebaceous glands. It was found that blocking the conversion of testosterone to dihydrotestosterone by effectively inhibiting the ductase activity.

In addition, it was confirmed that the use of volcanic stone clusters and cedar extracts, rape blossom extracts, bamboo extracts, green tea extracts, or mixtures thereof showed a higher inhibitory effect of 5α-reductase activity than volcanic stone clusters alone.

Therefore, the mixture of volcanic stone cluster and volcanic stone cluster and cedar extract, rape blossom extract, bamboo extract or green tea extract of the present invention has an excellent effect of inhibiting the activity of 5α-reductase, which is effective in suppressing the excessive secretion of sebum.

[Examples 1-6 and Comparative Example 1]

According to the composition described in Table 1 below, Examples 1 to 6 and Comparative Example 1 of the lotion formulation were prepared (unit: wt%).

Raw material name Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Comparative Example 1 1.Cetearyl Alcohol 1.0 1.0 1.0 1.0 1.0 1.0 1.0 2.lipophilic glyceryl stearate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 3. Glyceryl Stearate SE 1.5 1.5 1.5 1.5 1.5 1.5 1.5 4.phytoscualan 3 3 3 3 3 3 3 5.hydrogenated polydecene 2 2 2 2 2 2 2 6.Dimethicone 0.5 0.5 0.5 0.5 0.5 0.5 0.5 7.Polysorbate 60 One One One One One One One 8.Sorbitan sesquioleate 0.4 0.4 0.4 0.4 0.4 0.4 0.4 9.Methylparaben 0.1 0.1 0.1 0.1 0.1 0.1 0.1 10.propylparaben 0.05 0.05 0.05 0.05 0.05 0.05 0.05 11.purified water To 100 To 100 To 100 To 100 To 100 To 100 To 100 12.butylene glycol 5 5 5 5 5 5 5 13.Polyacrylate-13 * Polyisobutene * Polysorbate 20 0.5 0.5 0.5 0.5 0.5 0.5 0.5 14.Volcanic Rock Cluster One One One One One One - 15 Cedar Extract One - One - - - - 16.Rape Flower Extract One - - One - - - 17.Bamboo Extract One - - - One - - 18.Green Tea Extract One - - - - One -

<Production method of Examples 1 to 6 and Comparative Example 1>

1) The 11-18 components were uniformly mixed while heating to 70 degreeC, and the water phase part was produced.

2) The 1-10 components were uniformly mixed while heating to 70 degreeC, and the oil phase part was produced.

3) The oily part of 2) was added to the aqueous part of 1) and homomixed at 7,200 rpm for 6 minutes.

4) The mixture of 3) was cooled to room temperature.

[Test Example 2] sebum secretion inhibitory effect

In order to determine the sebum secretion inhibitory effect of Examples 1 to 6 and Comparative Example 1 was evaluated as follows. Ten subject men and women who felt sebum secretion were selected and the lotions of Examples 1 to 6 and Comparative Example 1 were applied to the designated sites every day for four weeks. The determination of the effect of sebum reduction was measured using a sebum meter, and the results are shown in Table 3 below.

Sebum secretion inhibitory effect Subject Average sebum reduction rate after 2 weeks Average sebum reduction rate after 4 weeks Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Comparative Example 1 Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Comparative Example 1 One 22 18 19.1 19.2 19.5 20.2 10 24 20 22 21.7 23 21.5 8 2 24 21 22.2 21.7 22.5 21.6 7 29 23 25.2 26.1 24.8 25.8 7 3 21 15 18.7 16.2 17.1 18.3 8 25 18 21.3 23 24.1 21.7 7 4 19 17 18.1 16.0 17.2 18.5 5 23 20 21.2 21.7 22 22.1 5 5 21 17 20.3 17.3 17.9 18.3 7 26 22 24 23.7 24.1 25.2 10 6 22 20 21 20.1 20.2 20.5 6 27 20 22.6 23 25 23 5 7 18 14 17.8 14.3 16.1 15.7 6 20 14 17.1 18.2 16.3 17.5 5 8 25 18 20.2 19.2 18.5 19.5 10 27 20 22.5 23.8 24.5 23.7 6 9 20 18 18.2 18.3 18.7 19.0 8 24 20 22.5 21.8 23.1 22.9 7 10 23 20 20.4 21.0 20.7 21.1 11 27 22 24.6 25.1 26.1 22.9 7 medium 21.5 17.8 19.6 18.3 18.8 19.3 7.8 25.2 19.9 22.3 22.8 23.3 22.6 6.7

From the results in Table 3, it can be seen that the cosmetic composition containing the volcanic stone cluster of the present invention as an active ingredient can effectively suppress sebum secreted in excess than Comparative Example 1 containing no volcanic stone cluster.

In addition, it was confirmed that the cosmetic composition containing both volcanic stone clusters and cedar extracts, rape blossom extracts, bamboo extracts, green tea extracts, or mixtures thereof exhibited a higher sebum secretion effect than Example 2 containing volcanic stone clusters alone. .

Therefore, the cosmetic composition containing the volcanic stone cluster of the present invention and the cosmetic composition containing the volcanic stone cluster and cedar extract, rape blossom extract, bamboo extract, green tea extract or a mixture thereof have an excellent sebum secretion inhibitory effect.

Test Example 3 Inhibition Effect of Reactive Oxygen Species Production

The keratinocytes (keratinocyte) isolated from the skin tissue of the person into a 5 × 10 ⁴ are in each well of a 24 well (well) type cell culture plate was attached for 24 hours. After 16 hours, 1% of volcanic stone clusters, cedar extracts, rapeseed extracts, bamboo extracts, and green tea extracts were treated. After 2 hours, the culture solution was removed, and 100 µl of phosphate buffered saline (PBS) was added to each well. The keratinocytes were irradiated with UV 30 mJ / cm 2 using an ultraviolet B (UV B) lamp (Model: F15T8, UV B 15W, Sankyo Dennki, Japan), and then the PBS was removed and each cell was cultured with keratinocytes. 200 μl was added. Here, volcanic ash, cedar extract, rapeseed flower extract, bamboo extract and green tea extract were retreated and the amount of reactive oxygen species (ROS) increased by UV stimulation at certain time periods was quantified. The amount of ROS was quantified by referring to Tan's method for measuring the fluorescence of DCF-DA (dichlorofluorescin diacetate) oxidized by ROS (Tan et al., 1998, J. Cell Biol. Vol. 141, pp1423-1432). The results are shown in FIG. In Figure 1, the ratio of the control ROS after 6 hours has been described as a numerical value.

In Figure 1, the mixture of volcanic stone cluster and volcanic stone cluster and cedar extract, rape blossom extract, bamboo extract or green tea extract effectively inhibited the generation of ROS known to cause skin cell damage by ultraviolet rays, and thus it was found to have excellent antioxidant efficacy.

 Test Example 4 Collagen Biosynthesis Promotion

The collagen biosynthesis-promoting effect of the mixture of volcanic and clustered ashes and cedar extracts, rape blossoms, bamboo extracts and green tea extracts was measured in comparison with tocopherol and EGCG.

First, fibroblasts were seeded at 10 ⁵ per hole in 24 wells and cultured until 90% growth. After culturing with serum-free DMEM medium for 24 hours, the volcanic rock cluster, cedar extract, rape blossom extract, bamboo extract, green tea extract, tocopherol, and EGCG dissolved in serum-free medium were treated at 10 ^-4 molarity. CO ₂ for hours Cultured in the incubator. These supernatants were removed and procollagen increased or decreased using procollagen type I ELISA kit (procollagen type (I)). The results are shown in Table 4, and the synthetic ability of collagen is compared with the non-treated group as 100.

division Collagen Synthesis (%) Untreated group 100 Tocopherol 113 EGCG 120 Volcanic Stone Cluster 125 Volcanic Stone Cluster + Cedar Extract (1: 1) 129 Volcanic Stone Cluster + Rape Blossom Extract (1: 1) 126.5 Volcanic Stone Cluster + Bamboo Extract (1: 1) 127 Volcanic Stone Cluster + Green Tea Extract (1: 1) 128 Volcanic Stone Cluster + Cedar Extract + Rape Flower Extract + Bamboo Extract + Green Tea Extract (1: 1: 1: 1: 1) 131

From the results of Table 4, the volcanic stone cluster according to the present invention was found to have a higher collagen synthesis than the positive control tocopherol and EGCG, volcanic stone cluster and cedar extract, rape blossom extract, bamboo extract, green tea extract or When using the mixture together it was confirmed that the improved collagen synthesis ability.

Therefore, the mixture of volcanic stone cluster and volcanic stone cluster of the present invention and cedar extract, rape blossom extract, bamboo extract or green tea extract can reduce the enlarged pores by increasing the amount of collagen production around the pores.

Test Example 5 Pore Shrinkage Effect Test in Lino Mouse

Using 46 lino mice, 0.5 ml of Examples 1 to 6 and Comparative Example 1 were applied. The material was treated for 1 week and the dorsal part was biopsyed 24 hours after the last treatment. Epidermis is separated, soaked in 0.5% acetic acid, fixed in 10% formalin, and cut vertically to 6 mM. Stained with hematoxylin and eosin. The size of the pores was measured using a mechanical eyepiece micrometer.

matter Pore size (mm) Untreated group 67 Example 1 56 Example 2 61 Example 3 57 Example 4 59 Example 5 58 Example 6 58 Comparative Example 1 68

From the results of Table 5, it can be seen that the volcanic stone cluster according to the present invention has an excellent effect of reducing the size of the pores, the volcanic stone cluster and cedar extract, rape blossom extract, bamboo extract, green tea extract or a mixture thereof In this case, it was confirmed that the effect of reducing the pore size was improved.

Test Example 6 Effect of Reduced Expression of Skin Inflammatory Factor

Enzyme Linked ImmunoSorbent Assay (ELISA) was performed to determine the effect of the volcanic stone cluster, cedar extract, rape blossom extract, bamboo extract and green tea extract on inhibiting the expression of skin inflammatory factors MMP-1 and PGE-2. (SE Dunsmore, et al., J Biol Chem, 271: 24576-24582, 1996).

Cells were incubated for 24 hours with yellow dust (0.1ppm) in a previously dispensed medium, and then exchanged with the medium, and further incubated for 24 hours by treating volcanic stone clusters, cedar extracts, rape blossom extracts, bamboo extracts, and green tea extracts, and recovering the media. To a 96-hole plate incubator. The primary antibody (monoclonal antibody) was treated and reacted at 37 ° C. for 90 minutes. The secondary antibody, anti-mouse immunoglobulin (alkaline phosphatase conjugated anti-mouse IgG) was reacted for another 90 minutes, washed with a buffer solution, and then washed with an alkaline phosphatase substrate solution (1 mg / ml p in diethanolamine buffer solution). -Nitrophenyl phosphate) was reacted at room temperature for 30 minutes and absorbance was measured at 405 nm using an absorbance photometer. Absorbance of the cell culture solution not treated with the active ingredient according to the present invention was used as a control. Expression inhibition effect of MMP-1 and PGE-2 was calculated by the following equation 1, the results are shown in Table 6 below.

Test substance MMP-1 expression inhibition rate (%) PGE-2 expression inhibition rate (%) Control group - - Volcanic Stone Cluster 59 63 Volcanic Stone Cluster + Cedar Extract (1: 1) 64 65 Volcanic Stone Cluster + Rape Blossom Extract (1: 1) 63 67 Volcanic Stone Cluster + Bamboo Extract (1: 1) 63 66 Volcanic Stone Cluster + Green Tea Extract (1: 1) 65 67 Volcanic Stone Cluster + Cedar Extract + Rape Flower Extract + Bamboo Extract + Green Tea Extract (1: 1: 1: 1: 1) 68 70

As can be seen in Table 6, the volcanic stone cluster of the present invention inhibited the expression of inflammatory factors MMP-1 and PGE-2 of the skin, the mixture of volcanic stone cluster and cedar extract, rape blossom extract, bamboo extract or green tea extract It was confirmed that the expression of skin inflammatory factors was more effectively suppressed than when the volcanic stone cluster was used alone. In addition, the mixture of volcanic stone cluster, cedar extract, rape blossom extract, bamboo extract and green tea extract had the highest inhibitory effect on the expression of skin inflammatory factors.

Therefore, it was found that the mixture of volcanic stone cluster and volcanic stone cluster and cedar extract, rape blossom extract, bamboo extract or green tea extract of the present invention is excellent in preventing skin inflammation and trouble by inhibiting the expression of skin inflammatory factors.

Claims (5)

Sebum control cosmetic composition containing volcanic stone cluster (Scoria) as an active ingredient. A cosmetic composition for reducing pores containing volcanic stone cluster (Scoria) as an active ingredient. Cosmetic composition for inhibiting skin problems containing volcanic stone cluster (Scoria) as an active ingredient. The composition according to any one of claims 1 to 3, wherein the composition is Cryptomeria japonica extract, Rassica camperstris (rapeseed) flower extract, Bamboo (Bambusa vulgaris) extract, and Camellia sinensis leaf extract. Cosmetic composition, characterized in that it further contains one or more selected from the group consisting of. The cosmetic composition according to claim 4, wherein the volcanic stone cluster, cedar extract, rapeseed extract, bamboo extract and green tea extract are contained in an amount of 0.0001 to 80.0 wt% based on the total weight of the composition.

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