KR20110081514A - Cosmetic composition for preventing skin aging containing the extract - Google Patents

Abstract

The present invention relates to an anti-aging cosmetic composition containing the extract of the medicinal herbs, more specifically, free radical scavenging activity, cell protective effect against free radicals and inhibitory effect of elastase and tyrosinase activity It relates to an anti-aging cosmetic composition containing an excellent extract of the extract.

Description

Anti-aging cosmetic composition containing knotgrass extracts}

The present invention relates to a cosmetic composition for preventing skin aging due to an excellent antioxidant action, and more particularly, free radicals and free radicals that promote the aging of the skin by containing the extract extracted from the leaves and stems of dried grass, as an active ingredient. The present invention relates to an anti-aging cosmetic composition having an effect of preventing skin aging by removing and inhibiting the activity of elastase that degrades elastic fibers and at the same time inhibiting the activity of tyrosinase.

In the 2000s, the world is entering an aging society at a very fast pace. According to the data compiled by the National Statistical Office, Korea has already entered the aging society with 7% of the elderly population over 65 years old in 2000, and it is predicted that in 2018, the elderly population over 65 years will reach 14%. Doing. This rapid increase in the elderly population means a change in future social life, and thus the quality of life must be prepared for improvement after 65 years of age. In line with this, various studies on aging are progressing, and the demand and desire of the elderly population for health food and cosmetics are steadily increasing along with improving diet and fitness management through proper exercise.

Aging is a series of phenomena in which mental and physical function declines over time, and research on various theories and factors that cause aging is being published. Among them, the most persuasive aging hypothesis is representative of the free radical theory that aging progresses due to damage of cells and organs in vivo by free radicals such as free radicals. Free radicals are very unstable materials with an odd number of electrons and are highly reactive and powerful oxidants that tend to forcibly take electrons from other materials around them and stabilize themselves. In particular, almost all metabolic processes in vivo are constantly undergoing oxygen-based oxidation-reduction reactions, resulting in the generation of free radicals. To effectively control this, superoxide dismutase (SOD) is used. Reducing systems such as glutathione peroxidase and catalase are at work. However, as aging progresses, the aforementioned antioxidant systems are unable to function. As a result, lipid components and DNA in the cell nucleus are destroyed, resulting in abnormal metabolic processes. In addition, the destruction of the global environment and the destruction of the ozone layer in the atmosphere are accelerated, resulting in the release of more free and higher energy UV rays from the sun, leading to the formation of excess free radicals in the skin as well as substrate metalloproteinases. (MMPs: matrix metalloproteinases) are promoted to increase the destruction of extracellular matrix (ECM) such as collagen and elastin, which make up the network structure of the dermal layer of skin, and hyaluronic acid, which maintains moisture. increase the photo-aging speed. Therefore, if the external environmental factors such as ultraviolet rays, active oxygen, pollutants, and stress that contribute to skin aging can be completely removed or they can be alleviated, exogenous aging can be fundamentally controlled. The same ideal anti-aging method has not been developed, and studies are being actively conducted to find or synthesize substances in nature that can effectively suppress or block these external factors.

A node is a yearly plant belonging to the genus A, and it has no hairs on its whole, its stem is split up, stretches upward, and its nodes are swelled. The height is about 10-40 cm, the leaves are short stalks, alternate, long oval, 2-4 cm long, hairless, and the middle veins are raised. The leaf is membranous, irregularly torn, and has a thin vein. In July-September, a bunch of small green flowers bloom on the underarms. Calyxes 5, elliptical, green or white or pinkish, stamens 6-8, pistils 3, fruits are aquatic, triangular, spreading, glossy. The flowering period is from June to July, young leaves are eaten as herbs, and outposts are used for medicinal purposes. It is widely used all over the world, and because of its various effects, it is used as a private herb in many countries. In particular, it is known that nudity has diuretic effect, blood pressure lowering effect, blood coagulation action, and uterine contraction action. It is known to be used in many other diseases.

The present inventors have tried to find a natural product having an anti-aging effect, as a result of confirming that the extract of the madipule has an antioxidant and inhibitory effect of elastase and tyrosinase activity, and completed the present invention.

Accordingly, it is an object of the present invention to provide a cosmetic composition for preventing skin aging having antioxidant and inhibitory effects on elastase and tyrosinase activity.

In order to achieve the above object, the present invention provides a cosmetic composition for preventing skin aging containing the extract as an active ingredient.

The cosmetic composition according to the present invention contains an extract of safe natural material, as a active ingredient, antioxidation, wrinkle improvement and the like of free radical scavenging action, free radical scavenging action, elastase inhibitory action and tyrosinase activity inhibiting action and the like. Excellent whitening effect and skin anti-aging effect.

1 is a graph showing the free radical scavenging activity of the extract Mardipul.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a skin anti-aging cosmetic composition containing the extract as an active ingredient.

The grasshopper extract used in the present invention may be prepared by extracting the grassweed outpost by a conventional plant extraction method, preferably using water, an organic solvent or a mixed solvent thereof. In addition, the organic solvent may be used by mixing at least one solvent selected from methanol, ethanol, glycerin, propylene glycol, butylene glycol, ethyl acetate and hexane.

In the present invention, as an extraction method using the extraction solvent, a general plant extraction method such as hot water extraction or organic solvent extraction may be used, and specifically, the dried grass or dried powder thereof is added to the extraction solvent. It is appropriate to immerse for 0.5 to 30 days at 4 to 30 ° C or to heat for 2 to 72 hours at 30 to 70 ° C with stirring if necessary. The amount of the solvent used for extraction is preferably 5 to 20 times (weight ratio) relative to the extraction raw material.

In addition, the cosmetic composition according to the present invention may contain the extract as the active ingredient as extracted as the active ingredient, the extract is concentrated under reduced pressure completely while recovering the solvent evaporated using a distillation apparatus equipped with a cooling condenser again. It may also contain in the form of the obtained dry powder.

In addition, the cosmetic composition according to the present invention may contain fractions obtained by stepwise treatment of hexane, ethyl acetate, butanol and water in a volume of 1 to 10 times by volume in a dry powder obtained by concentrating the extract of Madipuru under reduced pressure as an active ingredient. . For example, the dried powder obtained by concentrating the extract of Maldiflorum under reduced pressure was dispersed in water, and then fractionated by treating the same amount or 1 to 10 times the volume of n-hexane, and the obtained water layer was treated with n-hexane again. In the same way, ethylacetate and butanol are fractionated. At this time, each of the n-hexane layer, ethyl acetate layer, butanol layer and the finally obtained water layer was concentrated under reduced pressure while recovering the solvent evaporated using a distillation apparatus equipped with a cooling condenser, and then each dried powder was obtained. Can be used as an active ingredient.

The cosmetic composition for preventing skin aging according to the present invention may be prepared by containing 0.01% to 80% by weight based on the total weight of the composition, but preferably 0.01 to 50% by weight. When the knotweed extract is contained in less than 0.01% by weight, it is difficult to obtain the expected effect, when contained in more than 50% by weight may cause skin irritation problems. On the other hand, if the grass extract contains more than 80% by weight may cause problems in formulation stability.

The cosmetic composition for preventing skin aging according to the present invention is characterized by having an anti-aging effect of inhibiting the activity of free radicals, free radicals, elastase and tyrosinase by containing the extract as an active ingredient. In one embodiment of the present invention, the free radicals and free radical scavenging efficacy, elastase and tyrosinase activity inhibitory effect was confirmed by using the extract of the node.

Cosmetic composition for preventing skin aging according to the present invention is added to the additives or additives commonly used in the art in addition to the active ingredient, skin, skin toner, skin softener, skin lotion, astringent, lotion, nutrition lotion, milk Lotion, Moisture Lotion, Nutrition Cream, Massage Cream, Moisture Cream, Hand Cream, Essence, Nutrition Essence, Serum, Concentrate, Pack, Mask, Soap, Cleansing Foam, Cleansing Lotion, Cleansing Cream, Body Cleanser, Body Lotion, Body Oil, It may be formulated as a shampoo, rinse, foundation, makeup base, fact, loose powder, pressed powder, compact, eye shadow, eyeliner or lipstick and the like.

Hereinafter, the present invention will be described more specifically with reference to examples and test examples. These examples are provided only for understanding the contents of the present invention, and the scope of the present invention is not limited to these examples, and modifications, substitutions, and insertions commonly known in the art may be performed. This is also included in the scope of the present invention.

Example 1

300 g of dried dried grass leaves and stems were finely chopped, and then immersed in 3 L of 50% ethanol for 1 week and filtered. The filtrate was dried under a reduced pressure to obtain a dry powder, which was 5.42%.

[Example 2]

After the extract dried powder obtained in Example 1 was dispersed in purified water, the same volume of n-hexane was added, shaken and left for 2 to 3 hours at room temperature. The upper and lower layers were separated, and the supernatant (n-hexane layer) was separated. Recovered. The remaining lower layer solution was subjected to n-hexane fractionation by repeating the same procedure three times to remove nonpolar components. Ethyl acetate was added to the lower layer solution (water layer) thus obtained, and the mixture was left to stand at room temperature for 2 to 3 hours, then divided into an upper layer and a lower layer, and the upper layer solution (ethyl acetate layer) was recovered. The remaining lower layer solution was subjected to ethyl acetate fraction by repeating the same procedure three times. The extract, which was combined with the three fractions of the supernatant, was concentrated under reduced pressure to obtain 1.35% of dry powder relative to the weight of the dried pool grass.

Example 3

A part of the dry powder obtained in Example 2 was put in acetone solution in the presence of sulfuric acid and reflux-cooled while heating in a bath for 4 hours. The refluxed solution was neutralized and titrated with methanol solution containing 5% KOH, and then fractionated with ethyl acetate, and the ethyl acetate layer obtained was concentrated under reduced pressure to obtain 0.52% of dry powder by weight of bark grass.

Test Example 1 Free Radical Scavenging Activity Using DPPH Method

Free radical scavenging activity was measured for the extract of the Madipules using the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical. In the experimental method, 1 mL of the solution of 3, 9, 15, 30, 75, and 150 μg / mL of the Madipula extract prepared in Examples 1 to 3 was added to 1 mL of 0.2 mM DPPH solution dissolved in ethanol, respectively, and room temperature. After standing for 10 minutes at, the absorbance was measured at 517 nm using an absorbance spectrometer. At this time, the final concentration of the extract was 1, 3, 5, 10, 25, 50 ㎍ / mL, the free radical scavenging activity (%) for each concentration is shown in FIG.

The size of the free radical scavenging activity was shown as the control group when no sample was added and the inhibitory activity of DPPH was shown as the experimental group with the extract of bark grass. In addition, tocopherol ((+)-α-tocopherol), widely known as an antioxidant, was used as a positive control for comparison with the examples. DPPH scavenging activity was expressed as the concentration of the sample (FSC50, μg / mL) required to reduce the concentration of DPPH by 50%, the results are shown in Table 1 below.

Test substance Free radical scavenging activity
(FSC50, μg / mL) Example 1 19.08 ± 1.18 Example 2 5.25 ± 0.05 Example 3 5.92 ± 0.20 (+)-α-tocopherol 8.98 ± 2.93

In the results of Table 1, it was confirmed that free radical scavenging activity of Examples 2 and 3 among the extract of the node according to the present invention is superior to tocopherol, a positive control group.

Test Example 2 Cytoprotective Effect Using Photohemolysis

Experiments on cell damage and destruction by free radicals of human erythrocytes have many advantages as a model of cell damage by free radicals. Using this, the cytoprotective effect on free radicals was determined for the extracts of Example 1-3.

Erythrocytes were obtained from healthy adult men and women and immediately collected into heparin-added test tubes, centrifuged at 3,000 rpm for 5 minutes to separate erythrocytes and plasma, and the isolated erythrocytes were 0.9% saline phosphate buffer. ) Centrifuged to remove leukocyte layer. Red blood cells washed and separated three times were used while being stored in a refrigerator at 4 ℃, all experiments were carried out within 12 hours after blood collection. Photohemolysis experiments were performed according to already established methods. The erythrocyte suspension used in this experiment had an OD of 0.6 at 700 nm with an erythrocyte count of 1.5 × 10 ⁷ cells / mL.

3.5 mL of a red blood cell suspension was placed in a Pyrex test tube (No. 9820), and the solution of the grass extract of Examples 1-3 and (40), 80, 200, 400, and 800 μg / mL of ((+)-α-tocopherol was dissolved in ethanol. 50 μL of each was added, and the final concentrations of the node extract and tocopherol used were 0.5, 1, 2.5 5, and 10 μg / mL, and the photosensitizer was pre-incubated for 30 minutes in a dark place. 0.5 mL of rose-bengal (12 μM) was added and the entrance was blocked with a Parafilm (Whatman laboratory sealing film, UK) and then irradiated for 15 minutes.

The light irradiation required for light hemolysis was carried out by placing a 20 W fluorescent lamp in a 50 cm × 20 cm × 25 cm black box with a black interior and arranging a Pyrex test tube containing a red blood cell suspension at a distance of 5 cm from the fluorescent lamp to be parallel to the fluorescent lamp. It was then irradiated for 15 minutes. The degree of erythrocyte destruction according to the post-incubation time at 700 nm at the interval of 15 minutes after the light irradiation was calculated by the transmittance (%) of the formula (fraction of radiation transmitted through the solution).

Where P ₀ is the intensity of the incident light beam and P is the intensity of the transmitted light beam.

All experiments were carried out in a room temperature room temperature 25 ℃, the effect of the extract on the hemolysis of the hemolysis of the post-incubation time (hemolysis) from the graph consisting of 50% of the red blood cells hemolysis time τ ₅₀ was compared.

The control group showed good reproducibility in the experiments in all cases with τ ₅₀ within 31 minutes. Hemolysis did not occur until 120 minutes of cancer reaction in both the case of adding rose bengal and not irradiated with light and without adding rose bengal. All experiments were averaged three times and the results are shown in Table 2 below.

Inhibitory Effects of Radical Extracts on Photohemolysis of Human Red Blood Cells Test substance τ ₅₀ (there is a red blood cell destruction 50% of the time it takes ¹⁾⁾ 1 μg / mL treatment 5 μg / mL treatment 10 μg / mL treatment Example 1 32.44 ± 2.14 50.08 ± 7.84 101.25 ± 5.30 Example 2 43.69 ± 3.86 190.07 ± 10.35 314.70 ± 6.38 Example 3 50.28 ± 0.67 172.37 ± 4.41 264.59 ± 8.42 (+)-α-tocopherol - - 38.00 ± 1.80 ¹⁾ control, τ ₅₀ = 31.00 ± 1.00 min

The time taken for the erythrocyte cells to break down by 50% (τ ₅₀ ) is greater as the cytoprotective effect of the test substance increases. In the results of Table 2, it was confirmed that all of the extracts of Example 1 to 3 according to the present invention inhibits erythrocyte destruction by active oxygen in a concentration-dependent manner, in particular the extracts of Example 2 and 3 are positive control group It was confirmed that there is an excellent cell protective effect than (+)-α-tocopherol used as.

Test Example 3 Measurement of Elastase Inhibitory Activity

In skin aging, especially in the formation of wrinkles, the action of free radicals and the destruction of the extracellular matrix by MMPs (collagenase and elastase, etc.) are considered as the main causes. Therefore, the activity inhibitory ability of elastase, one of the major MMPs, was evaluated for the extracts of Example 2 and 3.

7.5 μL of a solution in which the following test substance was dissolved in ethanol and buffer in 1,300 μL of a buffer containing 1.0 mM of N -succinyl- (Ala) ₃ - p -nitroanilide, an elastase substrate, in 0.12 M of Tris-Cl (pH 8.0). Pre-incubate at 25 ° C. for 10 minutes with 92.5 μL, then incubate at 25 ° C. for 10 minutes with 100 μL of elastase solution (final concentration 0.0025 U / mL) and absorbance at 410 nm Was measured. In the control group, 100 μL of the solvent used as the sample solution was added instead of the node extract. Blank was added 1,300 μL of 0.12 M Tris-Cl buffer instead of N -succinyl- (Ala) ₃ -p -nitroanilide buffer and the concentration was the same as the experimental group.

Inhibitory Effects of Extracts of Radix Fructus on Elastase Activity Test substance IC ₅₀ (μg / mL) Example 2 14.38 ± 1.89 Example 3 13.36 ± 1.08 Oleanolic acid 13.70 ± 0.90

In the results of Table 3, the madipule extract of the present invention can be seen that the IC ₅₀ value is very low, which means that the level of oleanolic acid used as a positive control group is very excellent in inhibiting the activity of elastase.

Test Example 4 Measurement of Tyrosinase Inhibitory Activity

It is well known that tyrosinase acts as a key enzyme in melanin production from L-tyrosine. Therefore, the inhibitory effect of tyrosinase activity was measured for Examples 2 and 3.

1.0 mL of L-tyrosine (0.3 mg / mL), 1.8 mL of potassium phosphate buffer (0.1 M, pH 6.8), 0.1 mL of a solution of the following test substance dissolved in ethanol, followed by incubation at 37 ° C. for 10 minutes. Incubated. 0.1 mL tyrosinase solution (1,250 units / mL) was added to the reaction mixture and incubated at 37 ° C. for 10 minutes, then the reaction mixture was placed in an ice bath to terminate the reaction and the absorbance measured at 475 nm. Inhibition of enzyme activity was calculated as blank without addition of tyrosinase. The magnitude of the activity was expressed as the concentration of the sample (IC ₅₀ , μg / mL) required to reduce the activity of 0.1 mL tyrosinase by 50%, and the results are shown in Table 4 below.

Inhibitory Effects of Radix Extracts on Tyrosinase Activity Test substance IC ₅₀ (μg / mL) Example 2 79.50 ± 1.02 Example 3 30.32 ± 1.65 Arbutin 105.10 ± 3.30

In the results of Table 4, the extracts of Example 2 and 3 according to the present invention showed an excellent inhibitory effect on tyrosinase activity, in particular, arbutin used as a positive control has an excellent inhibitory effect on tyrosinase activity. I could confirm it.

[Formulation Example 1 and Comparative Formulation Example 1]

A nutritious cream comprising the ingredients listed in Table 5 below was prepared by a general O / W emulsion preparation method (unit: wt%).

Looking more closely, stearic acid, cetostearyl alcohol, polysorbate 60, sorbitassesquioleate, cetylethylhexanoate and pentaerythrityl tetraethylhexanoate as oils are dissolved by heating at 75 ° C, The remaining components were warmed to 75 ° C. to prepare an aqueous phase. The oil phase was slowly added to the aqueous phase and homomixed at 7,000 rpm for 3 minutes, and then bubbles were removed and cooled to 30 ° C. to obtain a cream formulation. Formulation Example 1 is the addition of 10% by weight of Example 3 dissolved in butylene glycol in 1% by weight, Comparative Example 1 is not added to the same formulation in the same formulation.

ingredient Formulation Example 1 Comparative Formulation Example 1 Purified water Balance Balance Stearic acid 1.0 1.0 Cetostearyl alcohol 2.0 2.0 Polysorbate 60 1.0 1.0 Sorbitan sesquioleate 0.4 0.4 Cetylethylhexanoate 5.0 5.0 Pentaerythritol tetraethylhexanoate 5.0 5.0 Butylene Glycol Containing 0.2% Example 3 10.0 - glycerin 10.0 10.0 Spices a very small amount a very small amount antiseptic a very small amount a very small amount Polyacrylamide * C13-C14 Isoparaffin * Laures-7 2.5 2.5

Test Example 5 Skin Elasticity Increase Effect

In order to measure the change in skin elasticity when Formulation Example 1 and Comparative Formulation Example 1 were applied to the skin, a sample of 20 to 40-year-old female owners of 44 skin owners in a normal state of Formulation Example 1 On the other side, Comparative Formulation Example 1 was applied twice a day after washing the morning and evening to be used continuously for 8 weeks. The skin elasticity of the 44 patients was measured under specific measurement conditions (2 mm-opening size probe, 450 mbar-negative pressure, no presuction, mode 1) using a cutometer (Cutometer® (C + K, Germany)). The skin elasticity at 4 weeks (T ₄ ) and 8 weeks (T ₈ ) after the initial skin elasticity (T ₀ ) and the cosmetic cream of the subjects were measured, and the results are shown in Table 6 below.

Skin elasticity change Elasticity Formulation Example 1 Comparative Formulation Example 1 T ₀ T ₄ T ₈ T ₀ T ₄ T ₈ R2 0.52 0.67 0.78 0.50 0.59 0.65 R7 0.45 0.59 0.70 0.46 0.52 0.58

R2: skin's ability to deform = total elasticity

R7: percent elasticity compared to a complete standard

In the results of Table 6, it was confirmed that the cosmetic composition of Formulation Example 1 containing the node extract according to the present invention significantly increased the elasticity of the skin compared to Comparative Formulation Example 1.

Claims (6)

Cosmetic composition for preventing skin aging containing the extract as an active ingredient. The cosmetic composition for preventing skin aging according to claim 1, wherein the knapweed extract is extracted with water, an organic solvent or a mixed solvent thereof. The composition of claim 1, wherein the extract of the knotweed is extracted by dipping at 0.5 to 30 days at 4 to 30 ° C or by heating at 30 to 70 ° C for 2 to 72 hours. The cosmetic composition for preventing skin aging according to claim 1, wherein the extract is contained in an amount of 0.01 to 50% by weight based on the total weight of the composition. The cosmetic composition for preventing skin aging according to claim 1, wherein the composition is a composition for removing free radicals, scavenging free radicals, inhibiting elastase activity or inhibiting tyrosinase activity. According to claim 1, wherein the composition skin, skin toner, skin softener, skin lotion, astringent, lotion, nutrition lotion, milk lotion, moisturizing lotion, nutrition cream, massage cream, moisturizing cream, hand cream, essence, nutrition essence , Serum, Concentrate, Pack, Mask, Soap, Cleansing Foam, Cleansing Lotion, Cleansing Cream, Body Cleanser, Body Lotion, Body Oil, Shampoo, Rinse, Foundation, Makeup Base, Pact, Loose Powder, Pressed Powder, Compact, Eye Shadow , Cosmetic composition for preventing skin aging, characterized in that it is formulated with at least one selected from the group consisting of eyeliner and lipstick.

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