KR20090021947A - Manufacturing method of tree odor extract for reducing odor, according to the tree extract and rug - Google Patents

Abstract

The present invention relates to a method for producing a tree extract used for reducing the odor of the barn, and according to the tree extract and the rug spreading the tree extract, especially the sawdust or leaves of the tree mixed with distilled water and the mixed mixture is filtered Or distillation to obtain wood sawdust extract or leaf extract. The tree extract thus obtained can reduce the ammonia concentration of the barn, inhibit the growth of microorganisms present in the barn, and can be provided with environmentally friendly natural compounds that can remove the odor of the barn because the animal is preferred.

Description

Manufacturing Method For Woody Plant Extract For Reduced Odor Of Stable, Woody Plant Extract and Rug Using The Same}

The present invention relates to a malodor removing agent for removing malodors of livestock inhabiting livestock and a method for producing the same, and particularly, extracts having an odor removal effect from sawdust or leaves of trees, which are environmentally friendly materials.

Recently, as the odor prevention law regulated the odor of pig farms and other barns, interest in the barn odors of pig farms and farms raising livestock has increased. The odor problem of the barn should be improved and solved in order to improve the qualitative growth of the livestock raising industry and increase profits.

For example, odors in pig farms are produced in pig farms. In order to minimize them, the characteristics of odor generation, prevention of concentration increase in air, and movement should be fundamentally performed. The unique smell of pig farms inside or outside the pig house is not a single compound such as ammonia gas or hydrogen sulfide gas, but a mixture of various compounds. These odor compounds are gas, water vapor and dust, and the highest proportion of the gases is ammonia gas and sulfurous acid gas produced during anaerobic decomposition of manure, and volatile fatty acids produced during anaerobic decomposition are the substances which greatly affect odor. .

Figure 1 is a schematic diagram for explaining the internal space of the pig in the odor occurs in the prior art, as shown here, the odor component floats in the air and the weight is increased, the automatic feed down, such as the ventilation duct These continuously decompose and continuously produce gas of odor component.

At this point, deodorants used in livestock farms, including some pig farms, are artificially synthetic chemicals, most of which are artificially synthesized, which can cause progressive harm to livestock in the livestock farms. It causes pollution, and there is a problem that the strength should be further increased in order to enhance the effect.

Accordingly, there is an urgent need for a odor remover made of a natural compound that can increase the effect through the microbial decomposition inhibitory action at the same time to remove the odor.

An object of the present invention is to extract a natural compound having a malodor removal ability from the tree, and to use the extracted extract to remove the malodor of the barn. To this end, the present invention is first to establish a technique for extracting from the trees a substance having a odor removal ability and antibacterial action of the barn.

Another object of the present invention is to remove the odor of the barn while extracting a substance having an antibacterial activity from the sawdust of the tree or the leaves of the tree, to increase the effect through the microbial decomposition inhibitory action at the same time to remove the odor of the barn It is to provide a livestock odor remover by preparing a tree extract consisting of natural compounds.

Still another object of the present invention is to provide a new technique for applying the odor removal tree extract for the barn prepared as described above to the barn. That is, in applying the tree extract made of natural compounds to the barn, instead of spraying it on a conventional sawdust as it is, by newly producing a biodegradable absorbent composite pellet or wood, by spraying the tree extract according to the present invention This is to further enhance the effect.

The method for producing a tree extract according to the present invention for achieving the above object is characterized by first mixing the sawdust and distilled water of the tree, and by separating the extract by filtering the mixed mixture. That is, the present invention is to extract the odor extracting ability from the tree using the sawdust of the tree, for this purpose is to react the sawdust of the tree to be mixed with distilled water. Such a reaction is suitable for extracting an odor removing ability from the sawdust of the tree by reacting at a temperature of 120 ° C. or higher and 1 atm or higher with a general autoclave.

Another embodiment of the method for preparing a tree extract according to the present invention may be characterized in that the leaves of the tree are mixed with distilled water, and the extract is separated by distillation of the mixed mixture. This extracts the odor extract from the leaves of the tree, for this purpose is to distill by mixing the leaves of the tree with distilled water. Distillation can be carried out by a general steam distillation extraction apparatus, by distillation of the gaseous material obtained by heating the mixture with such a distillation to obtain a tree extract according to the present invention.

Another embodiment of the method for producing a tree extract according to the present invention is to mix the wood sawdust extract and tree leaf extract obtained from the sawdust of the tree as described above to obtain a new type of tree extract mixture. That is, the present invention comprises the steps of mixing the wood sawdust and distilled water, and filtering the mixed mixture to separate the wood sawdust extract; Mixing the leaves of the tree and distilled water, and distilling the mixed mixture to separate the tree leaf extract; And a step of mixing the separated tree sawdust extract and the tree leaf extract, wherein the step of separating the tree leaf extract is performed first and then separating the tree sawdust extract. It is also possible.

In addition, in the above-described method for producing a tree extract, the tree is not particularly limited, but all may be used as long as sawdust or leaves can be obtained from the tree, and more preferably, conifers, especially pine.

Another embodiment of the present invention is an extract prepared by the manufacturing method as described above, the concentration of the extract may be a odor reduction tree extract is characterized in that the range is 1.0 ~ 10.0%. The tree extract is made of natural compounds that can increase the effect through the microbial decomposition inhibitory action at the same time to remove the odor of the barn. The concentration of the extract may vary depending on the sterilization conditions of the wood sawdust or distillation of the leaves of the above-mentioned trees, but if it is out of the above range may be less odor removal efficiency or the accessibility and preference of livestock in the barn, It is desirable to maintain an appropriate concentration.

Furthermore, another embodiment of the present invention is also possible for the odor reduction rug in which the extract prepared by the manufacturing method as described above is sprayed onto pellets made of paper or pretreated wood. The paper pellet refers to a lump that is constantly dense by stirring with a blender after the paper is crushed into a fibrous form, and the pretreated wood refers to a finely pulverized product dried after the wood lumber is dissociated into a fibrous form by water vapor pressure. . The present invention is applied to the paper dense mass and finely sized wood crushed wood as the above-described wood extract to use as a livestock litter, thereby inducing a decrease in ammonia gas concentration in the livestock stall and a reduction in the number of microorganisms present in the litter. This is a naturally degradable absorption type, which tends to favor cattle barns over conventional sawdust.

Specific details of other embodiments are included in the detailed description and the drawings. Hereinafter, specific embodiments of the present invention will be described in detail.

As described above, according to the present invention, a technology for extracting a substance having an odor removal ability and an antibacterial activity from a barn can be established from trees, and an extract liquid composed of the extracted natural compounds can be used for removing the odor from the barn. The present inventors confirmed that the extract extracted from the leaves and sawdust of the tree has the ability to reduce the odor of the barn, especially pine leaf extract was found that the effect is superior to other extracts.

Through this, the present invention can be removed from the sawdust or leaves of the tree while being able to remove the odor of the barn from the sawdust or leaves of the tree, and at the same time to remove the odor of the barn and increase the effect through the microbial decomposition inhibitory action It is possible to provide a tree extract consisting of natural compounds, through which it is possible to provide a livestock odor removing agent.

In addition, the present invention can provide a novel technique for applying the odor removal tree extract for the barn prepared as described above to the barn. In other words, in applying the tree extract made of natural compounds to the barn, compared to the pine sawdust used in conventional piglet, the naturally degradable absorbent paper pellets and pretreated wood produced according to the present invention are lowered in ammonia gas concentration and litter in the barn. It was possible to induce a decrease in the number of microorganisms present in, and has a great effect on the reduction of odor in the barn.

Hereinafter, one preferred embodiment of the present invention will be described in detail with reference to the accompanying drawings. The invention may be better understood by the following examples, which are intended for purposes of illustration of the invention and are not intended to limit the scope of protection defined by the appended claims.

Example  1: Preparation of tree extract

Example  1-1: Preparation of Wood Sawdust Extract

3L of distilled water and 500 g of pine sawdust (20 mesh through 80 mesh jam, total weight basis) were added to a mixed triangle flask and stirred, and then reacted for 1 hour at 121 ° C and 1.2 atm using an autoclave. The mixture was allowed to stand at room temperature, cooled, filtered under reduced pressure using a G1 glass filter, and separated into a filtrate and a residue. The filtrate thus separated was used as a wood sawdust extract.

Example  1-2: Preparation of Tree Leaf Extract

Figure 2 is a cross-sectional view showing an example of the steam distillation extraction apparatus for distilling the tree leaves for the manufacture of the tree leaf extract according to the present invention, the present inventors distilled pine leaves using this to prepare a tree leaf extract. That is, 3L of distilled water and 500 g of pine leaves (based on the total weight) were mixed and introduced into the extracting unit of the steam distillation extracting apparatus as shown in FIG. 2, and volatilized steam was obtained from the collecting unit. It was two hours. In addition, the pine leaves to be used at this time were used as the pine leaves removed twigs. As an example of the steam distillation extraction apparatus, a method and apparatus for extracting an effective ingredient of a plant using steam treatment according to Patent Registration No. 10-476629 may be used.

Example  1-3: Mixed Extract Preparation of Wood Sawdust and Leaves

In another embodiment of the present invention, the pine sawdust extract prepared in Example 1-1 and the pine leaf extract prepared according to Example 1-2 were mixed in a 1: 1 (v / v) tree extract mixture Was prepared.

Example  1-4: Yield and concentration of tree extract

Figure 3 is a photograph showing the tree extract prepared according to Examples 1-1, 1-2 and 1-3. As shown here, the wood sawdust extract according to Example 1-1 was light brown, and the tree leaf extract and the tree extract mixture according to Examples 1-2 and 1-3 were almost colorless and transparent. It was a liquid.

On the other hand, the yield and concentration of the tree extract prepared according to the above example was measured, the results are as shown in Table 1, the extraction yield of pine sawdust extract was 12.1 ± 1.3%, the extract concentration was 7.5%. In addition, the extraction yield of pine leaf extract was 5.5 ± 0.9%, the extract concentration was 2.0%.

Table 1: Yield and concentration of tree extracts

Example  2: rug (paper Pellet  And pretreatment wood)

Example  2-1: Paper Pellet  Produce

1 kg of newspaper was mixed with 20 liters of tap water and allowed to stand at room temperature for 5 hours, followed by stirring until the paper was dissociated into fiber in water using a stirrer attached with a propeller (approximately 30 minutes), and this was put into a plastic filter net. And dehydrated for 20 minutes using a domestic dehydrator. The dehydrated newspaper fibers were prepared in pellet form by stirring for 30 minutes-1 hour using a Hobart mixer (Kitchen Aid, Model 5K5SS, USA), which was dried at room temperature for 12 hours and then dried for 8 hours in a 105 ° C dryer to remove moisture. Paper pellets were prepared by complete removal. The length of the pellets thus prepared was about 1.0-2.0 cm.

Example  2-2: Preparation of Pretreated Wood

The oak chips (2 × 3 × 0.5cm) were put into a steam explosion (ju, Hilim Hitec, Daegu) device, and water vapor was added to the sealed reaction section to adjust the water vapor pressure to 25 Kg / cm2, and then 10 After reacting for a minute, it was rapidly released to atmospheric pressure. The chip released was almost dark brown and dissociated into fibrous form. This was dried for 24 hours in a dryer at 105 ℃ to remove the water, and then pulverized using a sawdust maker to collect only 20mesh passing through, used as a pretreated wood according to the present invention.

Example  2-3: Rug (Paper Pellet  And pretreated wood)

4 is a paper pellet (FIG. 4B) and pretreated wood (FIG. 4B) prepared according to Examples 2-1 and 2-2 together with pine sawdust (A in FIG. 4) used as a conventional barn rug. It is a photograph showing an example of C).

Table 2 below summarizes the physicochemical properties of the three types of mat as shown in FIG. According to the results, the water absorption rate of the paper pellets was almost similar to that of the control pine sawdust, the pH was 9.0 in the alkaline region, and the inorganic content was 7.6% higher than that of the other two samples.

Table 2: Characteristics of Rug

Example  3: application to extracts and rugs

5 is a schematic diagram for explaining a state in which the tree extract according to the present invention is applied to the barn. As shown here, pine extracts prepared according to Examples 1-1, 1-2 and 1-3 according to a certain area, using a manual sprayer on the bottom of the place where the conventional sawdust (pine) rug is present And evenly sprayed in an amount of 750 mL / m ² . In addition, an existing sawdust rug not sprayed with the extract according to the present invention was used as a control.

6 is a schematic diagram for explaining a state in which the paper pellet and the pretreated wood according to the present invention is applied to the barn. As shown here, the present inventors used a conventional pine tree which is a control with paper pellets (1500 g / m ² ) and pretreated wood (3500 g / m ² ) prepared according to Examples 2-1 and 2-2 above. Sawdust was applied to the bottom of the loin along certain areas. Then, the pine leaf extract prepared according to Example 1-2 was evenly sprayed on the bottom of the piglet.

Example  4: extract and rug Odor reduction  Efficacy Evaluation Method

In order to evaluate the odor removal efficacy of the tree extract and the rug according to the present invention, pH, ammonia gas concentration, animal habit, and microbial population were analyzed by the following method.

1. pH measurement

The pH was measured using a pH meter (HI-8418, Hanna instruments) by taking 10 g of the bottom of the piglet, mixing with 100 mL of distilled water, stirring for 10 minutes, separating the supernatant.

2. Ammonia Gas Concentration

Ammonia gas concentrations were measured using a portable combined gas meter (QRAE +, RAE Systems, USA) at a 50 cm vertical position on the floor of the pig.

3. Animal Behavior Analysis

Using a digital camera, pigs were photographed before and after application for 12 hours, 24 hours, and 48 hours to analyze their preferences for the samples.

4. Microbial Population Analysis

After the plated litter sample with ^{10 5 cfu / g, 2 ~} - it was used for dilution flatness malbeop for microbial populations analyzed, to follow each of the microbial species was used as the medium shown in Table 3, 10 ^second on each medium Colonies formed by incubation for 3 days (mold is cultured for 6-8 days) were counted.

Table 3: Culture Medium and Medium Composition According to Microbial Species

Microbial species Culture medium Badge composition Bacillus sp. MYP agar Beef extract 1g, Peptone 10g, D-mannitol 10g, Sodium chloride 10g, Phenol red 0.025g, Agar 15g Distilled water 1L Lactobacillus sp. Lactobacill MRS agar Proteose peptone No.3 10g, Beef extract 10g, Yeast extract 5g, dextrose 20g, Polysorbate 80 1g, Ammonium citrate 2g, Sodium acetate 5g, Magnesium sulfate 0.1g, Manganese sulfate 0.05g, Dipotassium phosphate 2g, Agar 15g Distilled water 1L Yeast sp. Sabroud dextrose agar Bacto Neopeptone 10g, Bacto Dextrose 40g, Bacto agar 15g Distilled water 1L Bacteria Pseudomonas agar Peptone 1.4g, Magnesium chloride 10g, Potassium sulfate 10g, Agar 15g Distilled water 1L Mold Potato dextrose agar Diced potatoes 300. 0 g Glucose 20. 0 g Agar 15. 0 g Distilled water 1.0 L

Example  5: of extract Odor reduction  efficacy

7 is a result of spraying the pine sawdust extract, pine leaf extract and pine extract mixture according to the present invention on a sawdust rug used in general pigs, and the pH change of the rug over time. As shown here, the pH of the rug sprayed with sawdust extract, leaf extract and extract mixture according to the present invention was 7.1 to pH 7.8, which was not different from the control (no spray of the tree extract).

Figure 8 is a graph showing the amount of ammonia gas generated over time the three pine extract and the mixed solution sprayed in accordance with the present invention. As shown here, after 6 hours and 12 hours, the amount of ammonia gas generated was significant at 5% level of all three extracts, and the pine leaf extract showed the lowest ammonia gas concentration. After 24 hours and 48 hours, the pine ammonia extract showed the lowest ammonia gas concentration and was significant at the 5% level. That is, as a result, when the pine sawdust extract and the leaf extract was sprayed on the pig rug, the ammonia gas concentration could be reduced. Therefore, the pine sawdust extract and the leaf extract according to the present invention can confirm that the odor removal ability is possible. there was.

9 is a graph showing the number of microorganisms present in the rug after spraying the pine extract according to the present invention on the rug in the pig room, 12 hours. As shown here, most of the microorganisms present in the rug was Bacillus and bacteria, the microbial population was the lowest in all kinds of microorganisms when the pine extract mixture according to Example 1-3 is applied, Significance was found at the 5% level. Accordingly, when the pine extract mixture according to the present invention is applied to the pig rug, the number of microorganisms present in the rug is the least, and the pine extract has the ability to inhibit microbial growth, especially when the sawdust extract and the leaf extract are mixed. The effect was judged to be even better.

Figure 10 is a schematic diagram showing the distribution of pigs after a certain time (12 hours or 24 hours) after spraying the pine extract according to the present invention on the mat in the pig room, Figure 11 is a photograph thereof. Many experimental pigs were lying on a rug sprayed with pine leaf extract and pine extract mixture, and some were lying or sitting on a rug with sawdust extract and a control (sprayless). In other words, pigs do not completely avoid the tree extract according to the present invention, and among them, it can be seen that the pine leaf extract and the pine extract mixture according to the present invention are most preferred. These results can be confirmed that even if the pine extract according to the present invention applied to the pig rug, it will not significantly affect the movement of the pigs.

Example  6: rug (paper Pellet  And pretreated wood) Odor reduction  efficacy

12 is a graph showing the results of measuring the pH change of the rug over time of paper pellets and pretreated wood according to the present invention according to Example 3 applied as a rug in a barn. As shown here, the rug applied to the tree extract according to the present invention was neutral or weakly acidic before application, but after 6 hours, it showed weak alkali at pH 8.0 to 8.7 regardless of the type of rug. That is, it was confirmed that there is no pH change according to the type of rug.

FIG. 13 is a graph showing the amount of ammonia gas generated over time of a piglet in which a paper pellet and pretreated wood according to the present invention are applied as a rug in a barn. As shown here, up to 12 hours the ammonia concentration of the litter was lower than the control pine sawdust. Especially when paper pellets were applied, the ammonia gas concentration was lower than that of other rugs. However, after 24 hours, there was little difference in ammonia gas concentration by type of rug. Among them, when using the paper pellets according to the present invention as a rug, it was confirmed that the ammonia gas absorption capacity is much higher than the control pine sawdust.

14 is a graph showing the population of microorganisms present in the rug after 12 hours after applying the rug according to the present invention, the microorganism is mainly Bacillus sp. And Bacteria accounted for the majority. The smallest number of microorganisms was present in the paper pellet, followed by pretreated wood and control (pine sawdust). As a result, it was confirmed that when the paper pellets are used as a litter pad, the microbial growth was suppressed better than that of the control pine sawdust.

Fig. 15 is a schematic diagram showing the distribution of pigs when the rug according to the present invention is applied to a pig room, and Fig. 16 is a photograph thereof. As applied here, at 12 and 24 hours after application of the rug, pigs were distributed in paper pellets and pretreated wood rugs. Accordingly, even when pigs use paper pellets and pretreated wood as rugs, it was judged that there was no repellent symptoms. The paper pellets according to the present invention are considered to be superior to conventional pine sawdust as a rug material.

On the other hand, while the present invention has been shown and described with respect to certain preferred embodiments, the invention is variously modified and modified without departing from the technical features or fields of the invention provided by the claims below It will be apparent to those skilled in the art that such changes can be made.

The present invention can provide a tree extract made of a natural compound that can increase the effect through the microbial decomposition inhibitory action at the same time to remove the odor of the barn, it can provide a odor removing agent for livestock. In addition, the tree extract may be applied to a naturally degradable absorbent paper pellets and pre-treated wood may be provided as a livestock rug with better effectiveness than conventional sawdust.

1 is a schematic diagram for explaining an internal space state of a pig house where a bad smell occurs in the prior art,

2 is a cross-sectional view showing an example of a steam distillation extraction apparatus for distilling tree leaves for producing a tree leaf extract according to the present invention,

3 is a photograph showing an example of the tree extract prepared according to the present invention,

4 is a photograph showing an example of paper pellets (B of FIG. 4) and pretreated wood (C of FIG. 4) prepared according to the present invention, together with pine sawdust (A of FIG. 4) used as a conventional barn rug. ,

5 is a schematic diagram for explaining a state in which the tree extract according to the present invention is applied in the barn,

Figure 6 is a schematic diagram for explaining the state of applying the paper pellets and pretreated wood according to the present invention in the barn,

7 is a graph showing the results of measuring the change in the pH of the rug after spraying the pine sawdust extract, pine leaf extract and pine extract mixture according to the present invention on a sawdust rug used in general pigs

FIG. 8 is a graph showing the amount of ammonia gas generated over time when three pine extracts and a mixed solution are sprayed according to the present invention.

9 is a graph showing the microbial population present in the rug after spraying the pine extract according to the present invention on the rug in the pig room, 12 hours,

10 is a schematic diagram showing the distribution of pigs after a predetermined time (12 hours or 24 hours) after spraying the pine extract according to the present invention on the mat in the pig room,

11 is a photograph showing a pig distribution of FIG.

12 is a graph showing the result of measuring the pH change of the rug over time of paper pellets and pretreated wood according to the present invention applied as a rug in a barn,

FIG. 13 is a graph showing the amount of ammonia gas generated over time of a piglet in which a paper pellet and pretreated wood according to the present invention are applied as a rug in a barn,

14 is a graph showing the population of microorganisms present in the rug after 12 hours after applying the rug according to the present invention,

15 is a schematic diagram showing the distribution of pigs when the rug according to the present invention is applied to a pig room,

FIG. 16 is a photograph showing a pig distribution of FIG. 15.

Claims (7)

Sawdust of wood and distilled water is mixed, and the mixed liquid mixture is filtered to separate the extract from the barn odor reduction wood extract manufacturing method. A method of producing a tree extract for reducing odors of a barn, characterized in that the leaves of trees and distilled water are mixed, and the mixed solution is distilled to separate the extract. Mixing wood sawdust and distilled water, and filtering the mixed solution to separate the wood sawdust extract; Mixing the leaves of the tree and distilled water, and distilling the mixed mixture to separate the tree leaf extract; And Mixing the separated wood sawdust extract and the tree leaf extract solution; odor reduction wood extract manufacturing method comprising a. Mixing the leaves of the tree and distilled water, and distilling the mixed mixture to separate the tree leaf extract; And Mixing wood sawdust and distilled water, and filtering the mixed solution to separate the wood sawdust extract; Mixing the separated tree leaf extract and the wood sawdust extract solution; odor reduction wood extract manufacturing method comprising a. The method according to any one of claims 1 to 4, wherein the trees are pine trees. An extract prepared by the manufacturing method according to any one of claims 1 to 4, wherein the concentration of the extract is in the range of 1.0 ~ 10.0% odor reduction wood extracts characterized in that the barn. The odor reduction rug of a barn, characterized in that the extract prepared by the manufacturing method according to any one of claims 1 to 4 is sprayed onto pellets made of paper or pretreated wood.

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