KR20080099570A - Composition for prevention and treatment of dermatitis disease containing complex herbal extract as an active ingredient - Google Patents

Abstract

The present invention is for the prevention and treatment of dermatitis diseases containing the complex herbal extracts of Angelica, black bark, raw sulfur, licorice, gold and silver, windproof, Bupyeongcho, Yeongyo, Red Peony, Cheongung, Hyungae, Baekjap, Mint, Seowookak and Gold as active ingredients. In particular, the complex herbal extract of the present invention, when applied to NC / Nga mouse of spontaneous atopic dermatitis induction model, by confirming the effect of inhibiting atopic dermatitis and blocking TARC mRNA production in skin cell lines, dermatitis It can be usefully used as an external skin pharmaceutical composition and cosmetic composition for the prevention and treatment of diseases.

Description

Composition containing mixed herbal extract for preventing and treating dermatitis}

       Figure 1 is a diagram showing the schedule of experiments using NC / Nga mouse, atopic dermatitis induced model,

       Figure 2 is a view showing the back surface of the mouse coated with the composite herbal extract (SP-1),

        Figure 3 is a diagram showing a sample of skin tissue coated with a composite herbal extract (SP-1),

       Figure 4 is a diagram showing the proportion of the skin forming skin according to the administration of the complex herbal extract (SP-1),

       5 is a diagram showing the degree of inflammatory cell infiltration into the dermis following administration of the complex herbal extract (SP-1),

        Figure 6 is a diagram showing the epidermal thickness according to the administration of complex herbal extract (SP-1),

 Figure 7 is an analysis of the effect of the compound herbal extract (SP-1) on TARC mRNA production in skin cell lines by RT-PCR.

      The present invention is for the prevention and treatment of dermatitis diseases containing the complex herbal extracts of Angelica, black bark, raw sulfur, licorice, gold and silver, windproof, Bupyeongcho, Yeongyo, Red Peony, Cheongung, Hyungae, Baekjap, Mint, Seowookak and Gold as active ingredients. It relates to a composition.

      The skin of the human body is an indispensable organ for the maintenance of life, which physically and chemically protects the body from extraterrestrial and performs the biochemical functions necessary for metabolism of the whole body. All abnormalities that appear on human skin (including hair, hands, and toenails) are called skin diseases, or skin diseases. Since the skin covers the surface of the body, there are many opportunities for direct contact with stimuli or various pathogens from the outside, and are strongly influenced by the body. Furthermore, even minor changes in the skin can be seen by eye and touched by hand, and it is easy to examine pathological histologically by extracting a part of the lesion, to examine microorganisms, and to clearly diagnose the diagnosis of individual diseases. In contrast, their types are considerably more numerous than other organs, and their names vary in complexity.

      Relatively common skin diseases include atopic dermatitis, contact dermatitis, seborrheic dermatitis, urticaria, athlete's foot, psoriasis, psoriasis, herpes simplex / shingles, dry skin, housewives and acne. Among them, atopic dermatitis is a recurrent chronic dermatitis with severe pruritus. The cause of the disease is not clearly identified so far, but it is estimated that 10 to 20% of the world's population suffers from this dermatitis.

    In addition, the prevalence of atopic dermatitis continues to increase in recent decades due to the increase in allergens causing airborne allergens due to environmental pollution and the increase in conditions for forming dry skin.

     Atopy has the meaning of “out of place” or in Greek “strange”. Atopic dermatitis is characterized by erythema, swelling, severe pruritus and dryness, leaching and swelling, and in the acute phase forms blisters in the epidermis and chronic thickening of the skin that causes skin thickening. Dermatitis with specific clinical and chronic characteristics that occur is sometimes referred to as allergic eczema, pediatric fever, and neurodegenerative dermatitis.

        A total of 18 children in 100 Korean infants aged 0-4 years have atopic dermatitis, and 63.6% of all atopy patients are 0-9 years old. This suggests that chronic inflammatory diseases, such as atopy, are seriously occurring in children (National Institutes of Health Insurance Review and Evaluation, National Statistical Office; 2000-2003 data). In the recent decades, the prevalence of atopic diseases has increased considerably in developed countries, and in Korea, the number of atopic dermatitis patients has increased with the recent surge of allergic diseases. According to a survey of 43,045 elementary and junior high school students nationwide in 2000 by the Korean Academy of Pediatric Allergy and Respiratory Diseases, 24.9% of elementary school students and 12.8% of middle school students were diagnosed with atopic dermatitis. Is serious. The number of atopic dermatitis cases was 1.15 million nationwide in 2003, which is 2.4% of the nation's citizens, and the social cost is so high that medical expenses can reach 22.9 billion won. . In addition, the social cost is expected to exceed 60 billion won when the costs of oriental medical treatment and private therapy are combined.

Drug treatment of atopic dermatitis or rhinitis in both cases uses steroids, antihistamines, antibiotics, and the like. However, in the case of these preparations, the temporary therapeutic effect is seen, but has the disadvantage of recurring soon, and the corticosteroid, which is an corticosteroid, has excellent effects due to its strong anti-inflammatory and immunosuppressive effects, but its side effects are serious. For this reason, the utility of herbal remedies for pediatric chronic inflammatory diseases has attracted great attention. Advantages of herbal treatment include: 1) Clinical evaluation of human efficacy has already been established, and 2) methods and theoretical concepts for reducing toxicity and maximizing efficacy using the abdomen are accumulated clinically. It can be said that it has an effective effect on chronic and refractory diseases known to be difficult to treat. Therefore, attempts to treat pediatric atopic dermatitis using herbal medicines are frequent, and some of the herbal medicines are known to be clinically excellent.

Angelica Angelica gigas Nakai) is found in mountains and valleys of wetlands and is distributed throughout the country. It has the effect of swelling, chemostasis, blood, colloquial, landscaping, soothing. It treats body weakness, headache, dizziness, dysmenorrhea, abdominal pain, vaginal damage and intestinal constipation. , Titanium Valley Peron, Noda kenin, beta-cytokines are components such as are known glycerol (pp, et al., illustrates hyangyak Dictionary, Younglim per jeongboseop 411, 1989; et al ryugyeongsu, study on the coumarin component of Angelica gigas muscle, 11: 33-35, 1967) .

Moutan Cortex Radicis is Paeonia a root bark of suffruticosa) roots par ohnol (paeonol), par Ono side (paeonoside), par Ono fluoride (paeonolide), par sludge may contain Florin (paeoniflorin). (Information, Dohae Hyangjeok Dictionary , Younglimdang, pp.526, 1989)

       Rehmannia glutinosa Libschitz var.purpurea Makino uses fresh roots of perennial herbaceous plants belonging to the family Ginsengaceae as a medicine. Fresh root roots of fresh or seonjihwang, dried dried kohwanghwang, raw sulfur added to Hwangju (黃酒) steamed and sun dried 9 times repeated is also known as sujijihwang.

Licorice (甘草, Glycyrrhizae radix ) is a generally safe herbal medicine frequently applied in many combination prescriptions (Kamei, J., et al., Eur . J. Pharmacol . , 469 , pp 159-163, 2003). Perennial herbaceous root (root) is columnar, main root is very long, rough and large, the outer shell is reddish brown to dark brown. Root is sweet and is used as sweetener and herbal medicine. It is distributed in northeastern China, Siberia and Mongolia. The root and root of licorice contain triterpene system saponin and glycyrrhizin, which are 2-glucuron of glycyrrhizin acid. Acid glycoside, a sweet component of licorice. This glycoside has no blood but glycyrrhetin acid. Among the hydrolyzed constituents of licorice, uralene acid was extracted and it was proved that it was 18α-glycirrhetin acid. Dohaehyang Medicine Dictionary , Younglim History, pp684-687, 1988).

Geum is a bud of Lonicera japonica THUNB., Which contains about 1% of luteolin and inositol, and also saponins and tannins. Dohaehyang Medicine Dictionary , Younglimsa, pp939-940, 1988).

Windbreaks ( Glehnia littoralis ) are the roots of oil herb. The taste is sour and sweet, and the nature is warm. Windproof contains octanal, nonanal, cuparene and β-eudesmol as essential oils. It is also a coumarin and chromium-based compound that contains scopoletin, psoralen, imperatorin, and the like. It is known to have an anti-inflammatory and analgesic effects (jeongboseop et al., Illustrates hyangyak Dictionary, Younglim four, p428, 1998).

Bupyeongcho ( Spirodela polyrrhiza (L.) SCHLEID.) Contains substances such as potassium acetate and potassium chloride, oxo, oval and the like. These polysaccharides are a rich source of d-apiose. Other resin (樹脂), napjil (蠟質), sterols (sterol) flow, which contains the chlorophyll, such as glycoproteins (糖蛋白質), clay, tannins (outer jeongboseop, illustrated hyangyak Dictionary, Younglim four, p277, 1998)

Fellowship ( Forsythia koreana Nakai is the fruit of forsythia, and fruit includes forsythol, sterol compounds, saponins, and flavonol glycosides. ), Meteresinoside, etc., and the rind contains oleanolic acid. Cheongyeon Bridge contains 4.89% of saponin and 0.2% of alkaloid and contains phylligenin, pinoresinol, bisepoxylignan, etc. jeongboseop others, illustrate hyangyak Dictionary, Younglim four, p974-975, 1998).

Peony ( Paeonia The roots of lactiflora PALL contain paeoniflorin, paeonol, paeonin, essential oils, fatty oils, resins, tannins, sugars, starch, mucus, β-sitosterol and triderpenoids. (Informatics et al., Dohae Hyangjeomsa Dictionary , Younglimsa, p523, 1998).

Cnidium The components of officinale MAKINO are known as cnidilide, ligustilide, neocnidilide, butylphthalide, and sedanonic acid. , Dohae Hyangjeok Dictionary , Yeonglimsa, p418, 1998).

Penguin (荊芥, Schizonepeta tenuifolia var. japonica KITAGAWA uses sun-dried herbs of annual herb, belonging to Labiatae . The oil contains 1.8% of essential oils, and d-menthone, dl-menthone and a small amount of d-limonene are included in the essential oil. It has the effect of Hae-pyo, Geopung, and Bleeding (Information et al., Dohae Hyangjeomsa Dictionary , Younglimsa, pp863-4, 1998).

Albino silkworm ( Bombyx mori L.) was _erected by an infection with Beauveria bassiana V _UILL before sedimentation and _contained 6.34% ash, 67.44% protein, 4.38% fat, and 11.31% moisture. Efficacy in treating ankylosing wind diseases and removing wind fever (Professor of Herbology , Herbology , Younglim History, pp.505-506).

Mint ( Mentha arvensis L. var. piperascens MALINV.) Fresh leaves contain 0.8 to 1% essential oils. The main component of the water-in-oil (油中) contains at about 77 ~ 78% menthol, and then the containing 8-12% by menthone (jeongboseop et al., Illustrates hyangyak Dictionary, Younglim four, pp851-852, 1998).

Right bundle branch number (the number of right bundle branch was used as a substitute for each Nishi (Rhinoceri Cornu) (bakseondong addition, the configuration principle of the Chinese medicine prescription, Younglim four, p.127) as a horn of buffalo, Nishi angle of the efficacy of the hemostatic ryanghyeol, reconciliation decrypting (Professor of Herbology , Herbology , Younglim History, pp.189).

Golden ( Scutellaria The roots of baicalensis GEORGI contain becaine, baicalin, wogonin, wogonoside and neoboicalein and again contain β-sitosterol. , Dohae Hyangjeomsa Dictionary , Younglim History, pp864-865, 1998).

         Therefore, the present inventors utilize the excellent dermatitis inhibitory effect of the extracts of Angelica, Angelica, lichen, licorice, gold and silver, windproof, Bupyeongcho, Yeongyo, Red Peony, Cheongung, Hyeonggae, Baekja sleep, peppermint, Seowoogak and Golden Complex Herbs, In order to alleviate, the present inventors attempted to demonstrate the usefulness of the skin coating method of the complex herbal extract. In addition, in the present invention, even when applied as a topical formulation to apply the complex herbal extracts to the skin, by confirming the distinct treatment of dermatitis in Nc / Nga mice, the present invention was completed.

   An object of the present invention is the prevention of dermatitis diseases containing the extracts of Angelica sinensis, bark skin, saengjihwang, licorice, gold and silver, windproof, Bupyeongcho, Yeongyo, red peony, cheonggung, hyeonggae, Baekja sleep, peppermint, seogeak and gold complex as an active ingredient The present invention provides a therapeutic external skin pharmaceutical composition and a cosmetic composition.

        In order to achieve the above object, the present invention contains the complex herbal extracts of Angelica sinensis, Mokjepi, raw jihwang, licorice, gold and silver, windproof, Bupyeongcho, Yeongyo, Red Peony, Cheongung, Hyeonggae, Baekjajam, Mint, Seoggak and Gold Provided is an external skin pharmaceutical composition for preventing and treating dermatitis diseases.

        In addition, the present invention prevents and improves the dermatitis disease containing the complex herbal extracts of Angelica sinensis, black bark skin, saengjihwang, licorice, gold and silver, windproof, Bupyeongcho, Yeongyo, red peony, cheonggung, hyunggae, Baekja sleep, peppermint, Seowookak and gold as an active ingredient. It provides a cosmetic composition for.

        Extracts as defined herein include water, including purified water, lower alcohols having 1 to 4 carbon atoms or mixed solvents thereof, hexane, propylene glycol, butylene glycol, ethyl acetate, glycerin, preferably extracts soluble from water. .

       As a dry blending weight ratio of the mixed herbal extracts Angelica 15 ~ 50: Mokpipi 15 ~ 50: Saengjihwang 15 ~ 50: Licorice 5 ~ 30: Geumeunhwa 5 ~ 30: Windproof 5 ~ 30: Bupyeongcho 5 ~ 30: Yeongyo 5-30: Jeokjak 5-30: Cheongung 5-30: hyeonggae 5-30: Baekgang sleep 5-30: mint 2-10: Seogak 2-10: weight ratio of 2-10 gold, preferably donkey 15-16: silk skin 15-16 : Saengjihwang 15 ~ 16: Licorice 5 ~ 6: Geumeunhwa 5 ~ 6: Windproof 5 ~ 6: Bupyeongcho 5 ~ 6: Yeonkyo 5 ~ 6: Jeonjak 5 ~ 6: Cheongung 5 ~ 6: Hyunggae 5 ~ 6: Baekgangjam 5 ~ 6: mint 2 ~ 3: wooge 2 ~ 3: includes the weight ratio of 2 ~ 3 gold.

        The dermatitis disease includes at least one disease selected from atopic dermatitis, contact dermatitis, seborrheic dermatitis, skin pruritus, housewife eczema, erythematosis or psoriasis, preferably atopic dermatitis or contact dermatitis.

         Hereinafter, the method of obtaining the complex herbal extract of the present invention will be described in detail.

        Complex herbal extract of the present invention is the herbal medicines Angelica, bark skin, raw jihwang, licorice, gold silver flower, windproof, Bupyeongcho, Yeongyo, Red Peony, Cheongung, Hyeonggae, Baeksijam, peppermint, seowookak and gold after washing the water, dry and cut the above About 2 to 8 times the weight of the herbal material, preferably about 3 to 4 times the volume of water, for about 1 to 1 day, preferably about 3 hours at an extraction temperature of 90 to 100 ℃, preferably 100 ℃ Extraction method of cold sediment extraction, hot water extraction, ultrasonic extraction, reflux cooling extraction, etc., preferably extracted by hot water extraction method, the extract is filtered after cooling and the filtrate is dried with a lyophilizer to obtain the complex herbal extract of the present invention have.

        The present invention provides a skin external pharmaceutical composition for the prevention and treatment of dermatitis diseases containing a complex herbal extract obtained by the above production method as an active ingredient.

        The composition for the prevention and treatment of dermatitis diseases of the present invention comprises the extract in an amount of 0.1 to 50% by weight based on the total weight of the composition.

       The external dermal pharmaceutical composition containing the complex herbal extract of the present invention as an active ingredient may further include appropriate carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions.

       The external skin pharmaceutical composition containing the complex herbal extract according to the present invention as an active ingredient is in the form of external preparations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and sterile injectable solutions according to conventional methods, respectively. It may be used in a formulated formulation, and preferably provides an external skin pharmaceutical composition of a cream, gel, patch, spray, ointment, warning, lotion, linen, pasta or cataplasma.

       Carriers, excipients and diluents that may be included in the composition containing the complex herbal extract of the present invention as an active ingredient include lactose, dextrose, sucrose, oligosaccharide, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber , Alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil have. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and the solid preparations may include at least one excipient such as starch, calcium carbonate, sucrose in the extract. ) Or lactose, gelatin and the like are mixed. In addition to simple excipients, lubricants such as magnesium styrate talc are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups. In addition to commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, fragrances, and preservatives may be included. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

The composition for the prevention and treatment of dermatitis diseases containing the complex herbal extract of the present invention as an active ingredient is a skin external preparation formulation that can be applied to the skin, such as creams, gels, patches, sprays, ointments, warning agents, lotions, lining agents It may be used as a pharmaceutical composition in the form of external preparations for skin, pasta or cataplasma, but is not limited thereto.

  The preferred dosage of the composition containing the complex herbal extract of the present invention as an active ingredient depends on the condition and weight of the patient, the extent of the disease, the form of the drug, the route of administration and the duration, and may be appropriately selected by those skilled in the art. However, for the preferred effect, the composition of the present invention is preferably administered at 0.0001 to 100 mg / kg, preferably 0.001 to 10 mg / kg per day. Topical administration may be administered once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect.

        In addition, the present invention provides a cosmetic composition for the prevention and improvement of dermatitis diseases containing a complex herbal extract obtained by the above production method as an active ingredient.

        Complex herbal extract of the present invention can be used in a variety of cosmetics and facial cleansing and the like having an alleviating effect of dermatitis diseases. Examples of products to which the present composition can be added include cosmetics such as various creams, lotions, skins, and the like, cleansing agents, face washes, soaps, treatments, and essences.

        Cosmetics of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

        The water-soluble vitamins may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. Their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) may also be used in the water-soluble vitamins that can be used in the present invention. Included. The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.

          The oil-soluble vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol), and the like. , Derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherolvitamin E, DL-pantothenyl alcohol, D-pantotenyl alcohol, pantothenyl ethyl) Ethers, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

          The polymer peptide may be any compound as long as it can be incorporated into cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like can be given. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

         The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt, etc. can be normally purified from a mammal or fish.

         The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, phytosphingosine, sphingosaccharide lipid, etc. may be mentioned. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

         The seaweed extract may be any compound as long as it can be blended into cosmetics. Preferably, the seaweed extract may include brown algae extract, red algae extract, green algae extract, and the like. Also, calginine, arginic acid, sodium arginate, Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

          In addition to the said essential component, you may mix | blend the cosmetics of this invention with the other components normally mix | blended with cosmetics as needed.

          Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

         Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

         As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacate, adipine Acid isopropyl, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic penta erythritol Cetyl caprylate, lauric acid decyl, hexyl laurate, decyl myristin, myristin acid myristyl, myritic acid cetyl, stearyl stearate, decyl oleate, rininooleic acid Isostearyl laurate, isotridecyl myristin, isocetyl palmitate, cetyl, octyl stearate, isocetyl stearate, isodecate oleate, octylate dodecyl oleate, octyl dodecyl, isostearic acid isopropyl, 2 -Cetostearyl ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol dicacapate, capric acid Propylene glycol, dicapric acid neopentyl glycol, dioctanoate neopentyl glycol, tricaprylic acid glyceryl, triundecyl glyceryl, triisopalmitinate glyceryl, triisostearate glyceryl, neopentane dodecyl Isostearyl octanoate, octyl isononate, hexyl decyl neodecanoate, octyl dodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, Sothete octylate, polyglycerol oleate, polyglycerine isostearate, triisocetyl citrate, triisoalkyl citrate, triisoctyl citrate, lauryl lactate, myritic lactate, octyl lactate, octyl lactate, tricitric acid Ethyl, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipicate, diisopropyl sebacinate, Dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, physteryl isostearate, phytic oleate, 12-Steloylhydroxystearate isocetyl, 12-Steloylhydroxystearate stearyl, 12-steal One hydroxy stearic acid and the like esters such as cetearyl source.

         Hydrocarbon-based fats and oils, such as squalene, a liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, a liquid isoparaffin, polybutene, microcrystal wax, and a vaseline, etc. are mentioned as a hydrocarbon-type fats and oils.

          Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane, methylcetyloxysiloxane copolymer, dimethylsiloxane and methylsteoxysiloxane copolymer, and alkyl. Modified silicone oil, amino modified silicone oil and the like.

          Perfluoro polyether etc. are mentioned as fluorine-based fats and oils.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed oil, Palm oil, Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum oil, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba oil And animal or plant fats and oils such as candeler wax, carnava wax, liquid lanolin and hardened castor oil.

         Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

    Water-soluble low molecular humectants include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), polypropylene Glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

         Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

          Examples of the water-soluble polymer include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be.

          Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

         Examples of the emollient include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.

         As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

         As the nonionic surfactant, self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POE / POP (polyoxyethylene / polyoxypropylene) copolymer, POE / POP alkyl ether, polyether modified silicone, lauric acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

        Anionic surfactants include fatty acid soaps, alpha-acyl sulfonates, alkyl sulfonates, alkyl allyl sulfonates, alkyl naphthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, POE alkylphosphates, and alkylamides. Phosphates, alkyloylalkyltaurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinate salts, sodium alkyl sulfo acetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. have.

         As cationic surfactant, alkyl trimethylammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium bromide, behenyl trimethyl ammonium chloride, chloride Benzalkonium, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

        Examples of amphoteric surfactants include the carboxybetaine type, the amide betain type, the sulfobetain type, the hydroxysulfobetain type, the amide sulfobetain type, the phosphobetaine type, the aminocarboxylate type, the imidazoline derivative type, and the amideamine type. An amphoteric surfactant etc. are mentioned.

        Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine and composites thereof; Polyamide, Polyester, Polypropylene, Polystyrene, Polyurethane, Vinyl Resin, Urea Resin, Phenolic Resin, Fluorine Resin, Silicon Resin, Acrylic Resin, Melamine Resin, Epoxy Resin, Polycarbonate Resin, Divinylbenzene / Styrene Copolymer And organic pigments such as silk powder, cellulose, CI pigment yellow, and CI pigment orange, and composite pigments of these inorganic pigments and organic pigments.

         As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-epsilon-lauroyl-L-lysine, N-epsilon-palmitolyzine, N-alpha-paratoylol nitin, N-alpha-lauroyl arginine, N-alpha-cured fatty acid acyl arginine -Acyl basic amino acid; N-acyl polypeptides, such as N-lauroyl glycyl glycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylic acid and benzyl cinnamic acid. , Paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono-2-ethylhexane glyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, Urocanoic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenonedisulfonic acid sodium salt, dihydroxybenzo Phenone, tetrahydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino- p- (carbo-2'-ethylhexyl-1'-oxy)- 1,3,5-triazine, 2- (2-hi And the like can be mentioned hydroxy-5-methylphenyl) benzotriazole.

          As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitroguicol, undecylenic acid, and the like.

      Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

           Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

            Examples of the alcohol include higher alcohols such as cetyl alcohol.

          Moreover, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5% weight percentage with respect to a total weight, More preferably 0.01-3% by weight.

          The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

          Ingredients included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the compound as an active ingredient, for example, conventional auxiliary agents such as stabilizers, solubilizers, vitamins, pigments and flavorings. And carriers.

          The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, and includes, for example, milky lotion, cream, lotion, pack, foundation, lotion, essence, hair cosmetic, and the like.

         Specifically, the cosmetic composition of the present invention skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, Formulations of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

         When the formulation of the present invention is a paste, cream or gel, animal carriers, vegetable fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be.

         When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

         When the formulation of the present invention is a solution or emulsion, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

         When the dosage form of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

          When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

          Hereinafter, the present invention will be described in detail by the following Examples and Experimental Examples.

   However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following Examples and Experimental Examples.

Example  1.Combined herbal extract ( SP -1) Preparation

Purchased from Daewon Pharmaceutical Co., Ltd. (Daegu) 240g, dried and finely pulverized donkey 240g, raw paprika 240g, licorice 80g, gold and silver flower 80g, windproof 80g, Bupyeongcho 80g, Yeongyo 80g, red pepper 80g, Cheongung 80g, 80g, Baekgangjam 80g, peppermint 4,000 ml of water was added to 40 g, 40 g of Seogwak and 40 g of gold, and hot water was extracted for 3 hours at 100 ° C. After filtration using filter paper (3M), the filtrate was concentrated under reduced pressure using a lyophilizer (EYELA FDU-1100) and dried to obtain 232.6 g of the crude herbal extract, which was used as a sample of the following experimental example (hereinafter, “SP Named -1 ").

Reference Example  One. NC Of Nga  Fabrication of Dermatitis Mice

NC / Nga mice are well known as models for spontaneous atopic dermatitis. The cause of this was reported to be a result of IgE production due to the overactivity of JAK3 kinase, but the exact mechanism is still unknown. In spite of this mechanism ambiguity, it is the experimental model that best reflects atopic dermatitis in humans and is widely used in pharmacological experiments (Matsuda et al., Development of atopic dermatitis-like skin lesion with IgE hyperproduction in NC / Nga mice. Int Immunol , 9 , pp. 461-6, 1997). Despite these advantages, the rate of spontaneous dermatitis induction is low and the intensity of induced dermatitis is very different, making it a poor model for evaluation. In order to overcome this problem, Japan has solved this problem by artificially inducing dermatitis using TNCB (1-chloro 2,4,6-trinitro benzene), a hapten forming material. However, in the case of TNCB, domestic import is prohibited and cannot be used. In the present invention, DNCB (1-chloro 2,4-dinitrobenzene, Aldrich, USA), which is a structural analogue with TNCB, was used as the experiment schedule shown in FIG. 1. (See FIG. 1), a relatively constant atopic dermatitis was induced. NC / Nga mice used in the present invention were purchased from a central laboratory animal, and 5 week old male mice were used.

Reference Example  2. Complex herbal extract ( SP -1) Preparation of skin applicator containing

After freeze-drying the composite herbal extract (SP-1) prepared in Example 1 at -80 ° C, 1g, 5g and 10gdml SP-1 was diluted with dipropylen glycol (1: 1) for skin application. 1), 5%, and 10%, respectively, were dissolved in 100 ml of solvent, and 500 microl of each was applied to the NC / Nga mouse of Reference Example 1 for 4 weeks, and the therapeutic effect was observed in the following experimental example.

Experimental Example  1.Combined herbal extract ( SP -1) of atopic dermatitis inhibitory effect

Clinically used in atopic dermatitis in order to measure the inhibitory effect of atopic dermatitis after applying the SP-1-containing skin external preparation prepared in Reference Example 2 to the NC / Nga mouse prepared in Reference Example 1 according to the concentration. The experiment was performed as follows using the visual evaluation method.

       The evaluation items were erythema, pruritus & dry skin, edema & escoriation, erosion, and lichenification. ), Weakness (1), severity (2), and severeness (3), and scores of at least 0 and at most 15 are scored.

         The visual evaluation results were calculated as the total sum of the average scores after evaluating the five items in each animal, and the experimental results are shown in Table 1 and FIGS. 2 to 3.

Experimental group Erythema Itching & Dry Skin Edema & Hematoma Bark Taesun total NC / Nga DNCB 1.83 1.67 0.83 0 0.17 4.5 SP-1 (1%) 0.8 0.6 0.4 0 0 1.8 SP-1 (5%) 0.5 0.5 0.33 0 0 1.33 SP-1 (10%) 0.8 0.8 0.4 0 0.2 2.4 * None (0), weak (1), severity (2), severe (3)

      As a result, as shown in Table 1, the total score of SP-1 5% was the lowest as 1.33, it was confirmed that the atopic dermatitis inhibitory effect (see Fig. 2).

 In addition, the skin tissue was cut in a 1.5 X 1.5 cm 2 area around the affected area around the back and fixed in 10% formaldehyde solution and stored at 4 ℃ until tissue staining. The skin tissues were observed mainly on the overall condition of the skin, migration of inflammatory cells, and epidermal thickness. To this end, tissues were stained using hematoxylin-eosin staining, and observed and evaluated by a pathologist at a magnification of 100 times on an optical microscope, and a sample of skin tissue is shown in FIG. 3. It was. As shown in FIG. 3, it was confirmed that the skin formation and epidermal thickness in the epidermis were significantly inhibited when the SP-1 5% and 10% extracts were administered (see FIG. 3).

Experimental Example  2. Complex herbal extract using dermatitis pathology indicator SP -1) of atopic dermatitis inhibitory effect

In order to measure the inhibitory effect of atopic dermatitis upon concentration-specific treatment of the complex herbal extract (SP-1) using the dermatitis pathology indicator, the experiment was performed as follows.

  4 to 6 shows changes in the dermatitis index when blinding the skin of the experimental group to a pathologist (Department of Pathology, Chosun University College of Medicine). The indicators of skin formation (see FIG. 4), the degree of inflammatory cell migration into the dermis (see FIG. 5) and epidermal thickness (see FIG. 6) were increased by DNCB application, and combined herbal extracts (SP-1) 1, 5 and 10% Significant inhibition was observed upon treatment. However, the differential effects of concentration were not recognized. Each figure is expressed as mean ± standard error.

Experimental group  Rate of skin shaping (%) Control  9.3 DNCB  17.0 SP-1 (1%)  6.2 SP-1 (5%)  2.5 SP-1 (10%)  1.2

Experimental group  Inflammatory cell infiltration into the dermis Control  One DNCB  2.5 SP-1 (1%)  0.88 SP-1 (5%)  0.58 SP-1 (10%)  0.7 standard: (i) In the absence of inflammatory cells in the dermis: 0 (ii) In the presence of inflammatory cells in the dermis, limited to a few or very few observations: 1+ Multiple and commonly observed: 2+ In severe inflammatory cell infiltration: 3+

Experimental group  Epidermal thickness (mm) Control  0.10 DNCB  0.18 SP-1 (1%)  0.10 SP-1 (5%)  0.11 SP-1 (10%)  0.13

Experimental Example  3. HaCaT  human In skin cell lines  Complex herbal extract ( SP By -1) TARC mRNA  Measure generation inhibition

One of the representative ligands of CC chemokine receptor 4 (CCR4) is thymus- and activation-regulated chemokine (TARC). TARC affects the infiltration of Th2 cells via the CCR4 regulates T cell activity (Imai T, et al., The T cell-directed CC chemokine TARC is a highly specific biological ligand for CC chemokine receptor 4. J Biol Chem , 272 , pp. 1503642,1997).

TARC is produced in cutaneous keratinocytes and is known to be involved in dermatitis progression and exacerbation by promoting infiltration of Th2 cells (Vestergaard C, et al., A Th2 Chemokine, TARC, produced by keratinocytes may recruit CLA + CCR4 + lymphocytes into lesional atopic dermatitis skin. J Invest Dermatol, 115, pp.640-6, 2000).

       Therefore, as a possible mechanism of the atopic dermatitis inhibitory effect of the complex herbal extract (SP-1), the experimental process to quantify by the RT-PCR assay to measure the effect of the complex herbal extract (SP-1) on the production of TARC mRNA in skin cell lines Performed

       1.0 mg total RNA was reverse transcribed using oligo (dT) 20mer as a primer to synthesize cDNA, and the primers and reaction conditions are shown in Tables 5 and 6 below.

Human TARC mRNA Primer Sense (SEQ ID NO: 1) 5'-ACTGCTCCAGGGATGCCATCGTTTTT-3 '; 270 bp Antisense (SEQ ID NO: 2) 5'-ACAAGGGGATGGGATCTCCCTCACTG-3 '; 270 bp Reaction conditions  35 cycles of total dehydration at 98 ℃ for 10 seconds, annealing at 60 ℃ for 30 seconds, elongation at 72 ℃ for 1 minute

S16 ribosomal protein gene (control gene) Sense (SEQ ID NO: 3) 5'-TCCAAGGGTCCGCTGCAGTC-3 '; 152 bp Antisense (SEQ ID NO: 4) 5'-CGTTCACCTTGATGAGCCCATT-3 '; 152 bp Reaction conditions  35 cycles of total dehydration at 98 ℃ for 10 seconds, annealing at 60 ℃ for 30 seconds, elongation at 72 ℃ for 1 minute

       After electrophoresis on the agarose gel, the band was confirmed after staining with ethidium bromide (see FIG. 7).

  As shown in FIG. 7, TNF-alpha / IFN-gamma (20 ng / ml each) is an inflammatory source when the compound herbal extract (SP-1) was treated at 300 and 1000 microg / ml concentrations for 10 minutes before treatment. TARC mRNA production was completely blocked in HaCaT cells, a human skin cell line. This confirmed that the atopy blocking effect of the complex herbal extract (SP-1) is partially related to the chemokine production process.

         Examples of the formulation of the composition comprising the extract of the present invention will be described, but the present invention is not intended to limit it, but is intended to explain in detail only.

Formulation example  1. Flexible Lotion

Number Raw material Unit (wt%) One SP-1 4.0 2 glycerin 3.0 3 Butylene glycol 2.0 4 Polyoxyethylene (60) Cured Castor Oil 0.5 5 ethanol 6.0 6 Flavoring, preservative, coloring Quantity 7 Purified water Remaining amount Sum 100.0

Formulation example  2. Nutrition Lotion

number Raw material Unit (wt%) One SP-1 10.0 2 Squalane 4.0 3 Liquid paraffin 3.0 4 Caprylic / Capric Glyceride 4.0 5 Stearic acid 1.0 6 Cetyl alcohol 1.0 7 Glyceryl Monostearate 0.5 8 Polysorbate 60 1.5 9 Sorbitan sesquioleate 0.5 10 Glyceryl Stearate / Pig-100 Stearate 1.0 11 glycerin 3.0 12 Butylene glycol 2.0 13 Carbomer 0.1 14 Triethanolamine 0.1 15 Preservative, coloring, flavoring Quantity 16 Purified water Remaining amount Sum 100.0

Formulation example  3. Nutrition Cream

number Raw material Unit (wt%) One SP-1 10.0 2 Squalane 5.0 3 Liquid paraffin 3.5 4 Caprylic / Capric Glyceride 4.5 5 Stearic acid 1.5 6 Cetyl alcohol 2.0 7 Beeswax 1.0 8 vaseline 2.0 9 Glyceryl Monostearate 1.0 10 Polysorbate 60 1.5 11 Sorbitan sesquioleate 0.5 12 Glyceryl Stearate / Pig-100 Stearate 1.5 13 glycerin 5.0 14 Butylene glycol 5.0 15 Carbomer 0.2 16 Triethanolamine 0.2 17 Preservative, coloring, flavoring Quantity 18 Purified water Remaining amount Sum 100.0

Formulation example  4. Essence

number Raw material Content (% by weight) One  SP-1 0.5 2 Cytostolol 1.70 3 Polyglyceryl 2-oleate 1.50 4 Ceramide 0.7 5 Ceteares-4 1.2 6 cholesterol 1.5 7 Dicetylphosphate 0.4 8 Concentrated glycerin 5.0 9 Sunflower oil 15.0 10 Carboxy Vinyl Polymer 0.2 11 Xanthan Gum 0.2 12 antiseptic a very small amount 13 Spices a very small amount 14 Purified water Remaining amount system 100.0

Formulation example  5. Hydrophilic Ointment

number Raw material Content (% by weight) Content (% by weight) One   SP-1 0.5 - 2   SP-1 - 0.5 3   White vaseline 250 250 4   Stearyl alcohol 220 220 5   Ethyl (or methyl) p-oxybenzoate 0.25 0.25 6   Propylene glycol 120 120 7   Sodium lauryl sulfate 15 15 8   Propyl p-oxybenzoate 0.15 0.15

As described above, the composition containing the extracts of Angelica sinensis, bark skin, saenghwanghwang, licorice, gold and silver, windproof, Bupyeongcho, Yeongyo, Red Peony, Cheongung, Hyeonggae, Baekjap, Mint, Seogak and Gold as an active ingredient When applied to NC / Nga mice, a spontaneous atopic dermatitis induction model, by confirming the effect of inhibiting atopic dermatitis and blocking TARC mRNA production in skin cell lines, it is useful as an external skin pharmaceutical composition and cosmetic composition for the prevention and treatment of dermatitis diseases Can be used.

<110> chosun university <120> Composition comprising mixed herbal extract for preventing and          treating dermatitis <160> 4 <170> KopatentIn 1.71 <210> 1 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> TARC for sense primer <400> 1 actgctccag ggatgccatc gttttt 26 <210> 2 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> TARC for anti-sense primer <400> 2 acaaggggat gggatctccc tcactg 26 <210> 3 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> S16 for sense primer <400> 3 tccaagggtc cgctgcagtc 20 <210> 4 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> S16 for anti-sense primer <400> 4 cgttcacctt gatgagccca tt 22  

Claims (7)

Skin external pharmaceutics for the prevention and treatment of dermatitis diseases containing extracts of complex herbal extracts of Angelica, Peony, Saenghwang, Licorice, Geumgeunhwa, Windproof, Bupyeongcho, Yeongyo, Red Peony, Cheongung, Hyungae, Baekjajam, Peppermint, Seogak Composition. The method of claim 1, wherein the extract is soluble in a solvent selected from the group consisting of water including purified water, lower alcohols having 1 to 4 carbon atoms or a mixed solvent thereof, hexane, propylene glycol, butylene glycol, ethyl acetate, and glycerin. Skin external pharmaceutical composition as an extract.  According to claim 1, wherein the dry blending weight ratio of the crude herbal medicine is Angelica 15-50: Bark skin 15-50: Saenghwanghwang 15-50: Licorice 5-30: Geumeunhwa 5-30: Windproof 5-30: Bupyeongcho 5-30: Yeonkyo 5 ~ 30: Jeakpak 5 ~ 30: Cheongung 5 ~ 30: Hunggae 5 ~ 30: Baekgangjam 5 ~ 30: Mint 2 ~ 10: Seowoogak 2 ~ 10: Golden 2 ~ 10 weight ratio of the skin external pharmaceutical composition.  According to claim 1, wherein the dermatitis disease is atopic dermatitis, contact dermatitis, seborrheic dermatitis, skin pruritus, housewives, eczema or psoriasis pharmaceutical composition for external skin. The composition for external application for skin according to claim 1, wherein the composition is a cream, gel, patch, spray, ointment, warning, lotion, linen, pasta or cataplasma formulation. Cosmetic composition for the prevention and improvement of dermatitis diseases, containing the complex herbal extracts of Angelica, black bark, raw sulfur, licorice, gold and silver, windproof, Bupyeongcho, Yeongyo, Red Peony, Cheongung, Hyeonggae, Baekjap, Mint, Seogwi, and Gold.  According to claim 6, wherein the composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, Cosmetic composition, characterized in that the formulation selected from the group consisting of pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.

Images