KR20080092090A - Skin whitening composition containing lactic acid bacteria crushing liquid - Google Patents

Abstract

The present invention relates to a composition for skin whitening containing lactic acid bacteria lysates as an active ingredient. Since the composition of the present invention inhibits melanin production and suppresses the expression of enzymes involved in melanin production, it may be used in external preparations for skin, cosmetics, pharmaceuticals and / or health foods for skin whitening and improving effects.

Description

Whitening composition containing lactobacillus lysate}

Figure 1 is a schematic of the production method of lactic acid bacteria crushing liquid according to the present invention.

Figure 2 is a picture showing the effect of inhibiting melanin synthesis in the melanocyte lysate of the lactic acid bacteria lysate (LF; Lactobacillus fermentum , EF; Enterococcus ( Enterococcus) faecalis ), LC; Leuconostoc citreum ), LL; Lactococcus lactis ), LS; Lactobacillus sakei ), SM; Streptococus mutans , WC; Weisella cibaria )).

Figure 3 is a picture showing the inhibitory effect of lactic acid bacteria lysate mash mushroom tyrosinase activity according to the present invention.

Figure 4 is a picture showing the inhibitory effect of tyrosinase expression in the melanocyte lysate solution according to the present invention.

5 is a diagram showing the results of the cell stability test of lactic acid bacteria lysate according to the present invention.

The present invention relates to a functional composition containing a lactic acid bacteria lysate. More specifically, the present invention relates to a composition for skin whitening containing lactic acid bacteria lysate.

The composition can be used in external preparations for skin, cosmetics, drugs and / or health foods.

Skin is the body's primary protective layer that protects organs from changes in temperature and humidity, and from stimuli from the external environment such as ultraviolet rays and pollutants. However, with age, the secretion of various hormones that regulate metabolism decreases internally, the function of immune cells and the activity of cells decreases, and the ultraviolet content of the sun increases due to the destruction of the ozone layer. As environmental pollution becomes more severe, excessive physical and chemical irritation and stress caused by the increase of free radicals and active harmful oxygen decrease the normal function of the skin, produce melanin, blemishes, freckles, etc. Promotes damage to the skin.

In order to prevent such a phenomenon and maintain a healthier and more beautiful skin, by using physiologically active substances obtained from various animals, plants, microorganisms, etc. in the past, it is possible to maintain the inherent function of the skin and to activate skin cells by activating skin cells. Efforts have been made to effectively inhibit the deposition of melanin.

For example, hydroquinone, ascorbic acid, kojic acid, arbutin, and various extracts are used as a whitening cosmetic, but there are frequently problems that are degraded or colored, and their efficacy and effects are unclear. In particular, various extracts are very low in stability and are easily decomposed, and their efficacy and effects are unclear, and safety issues are steadily mentioned, and their use is limited.

Meanwhile, the use of microorganisms such as yeast and fungal bacteria in the cosmetic industry is increasing.

Lactobacillus is a bacterium that plays a beneficial role in the human body and produces lactic acid (lactic acid) by fermenting sugars such as carbohydrates. Up to 400 kinds of lactic acid bacteria have been discovered so far, and 18 of them are known to be commercially available. These lactic acid bacteria are known to have a physiological activity of immune enhancement, in particular, gastrointestinal immunity enhancement, and the consumption of fermented milk products is enormous. In particular, according to the recent well-being trend, as a social atmosphere that is good to eat good food is created, various cosmetic materials that are beneficial to the body are developed, and the culture of lactic acid bacteria increases the moisture of the skin and the turnover speed of the stratum corneum. Although the effect of reducing wrinkles has been reported, in many cases, the effect received when using cosmetics was insignificant.

In addition, conventionally, the product produced by fermenting and lactic acid bacteria are included in the cosmetic composition (Korean Patent No. 0030505), or a fermentation broth obtained by fermenting mushrooms, rice, fruit juice, plant extracts, etc. with a specific genus lactic acid bacteria in the cosmetic composition. And the like (Japanese Patent Laid-Open No. 2004-097999, Japanese Laid-Open Patent Publication No. 2005-298489). However, the skin whitening effect related to the fermentation broth of lactic acid bacteria and the lysate of lactic acid bacteria itself rather than the culture medium has not been known until now.

Thus, the present inventors cultured lactic acid bacteria, and in the case of lactic acid bacteria lysates obtained by crushing the lactic acid bacteria as well as the products produced by the lactic acid bacteria, blackening induced by α-MSLAN (α-melanocyte stimulating hormone) It strongly inhibits melanogenesis, inhibits melanin production, strongly inhibits induced tyrosinase expression, and strongly retains the effect of inhibiting the activity of mushroom tyrosinase, which was not expected in the prior art. The present invention was completed by confirming that there is an effect of skin whitening.

The present invention provides a composition for skin whitening containing lactic acid bacteria crushing liquid.

Lactobacillus crushing solution of the present invention refers to a material that can be obtained through the crushing process after obtaining the cells by culturing the lactic acid bacteria.

When the lactic acid bacteria lysate is prepared, the lactic acid bacteria are preferably cultured in the presence of oxygen.

In general, lactic acid bacteria can proliferate under both aerobic and anaerobic conditions, but under anaerobic conditions, their growth is inhibited, and during fermentation, they are affected by the growth of the cells, which reduces the cell mass and inhibits the production of secondary metabolites. . In the case of nasal bacteria, it is only anaerobes that can be grown under anaerobic conditions, and growth is inhibited under oxygen conditions. On the other hand, since the lactic acid bacteria used in the present invention are capable of proliferation under both aerobic and anaerobic conditions (cultivation), cultivation under oxygen conditions is more advantageous than culturing under anaerobic conditions. Thus, the present invention is characterized by culturing in the presence of oxygen using lactic acid bacteria which is advantageously cultured in the presence of oxygen.

The type of lactic acid bacteria in the present invention is not particularly limited, but Lactococcus sp .), Streptococcus sp .), Weisella sp. , Lactobacillus sp .), Enterococcus sp .) and Leuconostoc sp. can be used for lactic acid bacteria selected from the group consisting of. Preferably, Lactobacillus fermentum ), Enterococcus faecalis ), Leuconostoc citreum ), Lactococcus lactis ), Lactobacillus sakei ), Streptococus mutans ), and lactic acid bacteria can be selected from Weisella cibaria .

Lactic acid bacteria crushing solution of the present invention may be included in 0.001 to 30 weight ratio (w / w%), preferably 0.001 to 5 weight ratio (w / w%) based on the total weight of the composition.

According to the present invention, a composition for skin whitening containing lactic acid bacterium crushing liquid may be used in external preparations for skin, cosmetics, pharmaceuticals and / or health foods for skin whitening and improving effects.

Therefore, the composition of the present invention may further include an active substance having a known skin whitening effect as long as it does not deleteriously affect the skin whitening and improving effects other than the lactic acid bacteria crushing liquid.

As a result of performing a cumulative skin irritation test on the skin whitening composition according to the present invention, the lactic acid bacteria lysate was determined to be a safe substance for human skin (Example 4, Fig. 5). Therefore, it is suitable for use as an external preparation for skin. The formulation of the external preparation for skin is not particularly limited. For example, there are formulations such as powder, gel, ointment, cream, liquid, aerosol and the like.

In addition, the composition for skin whitening containing lactic acid bacteria crushing liquid according to the present invention can be used as a cosmetic. When used as the cosmetics, in addition to the lactic acid bacteria crushing liquid according to the present invention may be added a material optionally selected according to the formulation or purpose of use of other cosmetics. For example, purified water, oil, surfactants, moisturizers, higher alcohols, thickeners, chelating agents, pigments, fatty acids, antioxidants, preservatives, waxes, pH adjusters, flavoring agents and the like can be added.

The oils include mineral oil, cyclomethicone, squalane, octyldodecyl myristate, olive oil, macadamia nut oil, glycerol octanoate, castor oil, ethylhexyl isononanoate, dimethicone, cyclopentasiloxane, Sunflower seed oil and the like can be used.

The surfactants include sorbitan sesquinolate, polysorbate 60, glyceryl stearate, lipophilic glyceryl stearate, sorbitan oleate, sorbitan stearate, die-cetyl phosphate, sorbitan stearate / Sucrose cocoate, glyceryl stearate / polyethylene glycol-100 stearate, ceteareth-6 oleate, arachidyl alcohol / behenyl alcohol / arachidyl glucoside. Polypropylene glycol-26-butes-26 / polyethylene glycol-40 hydrogenated castor oil and the like can be used.

As the moisturizing agent, glycerin, sorbitol, propylene glycol, butylene glycol, hexylene glycol, diglycerin, betaine, glycerin-26, methylgluse-20 and the like may be used.

Cetyl alcohol, stearyl alcohol, octyldodecanol, isostearyl alcohol and the like may be used as the higher alcohol.

Xanthan gum, carbomer, magnesium aluminum silicate, cellulose gum, dextrin palmitate may be used as the thickener.

As the chelating agent, disodium idieti, tetrasodium idieti and the like may be used.

As the dye, Blue No. 1, Red No. 40, Yellow No. 4, Yellow No. 5, etc. may be used.

As the fatty acid, stearic acid, mystic acid, palmitic acid, isostearic acid, lauric acid, or the like may be used.

Tocopheryl acetate, butylated hydroxytoluene, etc. may be used as the antioxidant.

As the preservative, methyl paraben, butyl paraben, propyl paraben, phenoxyethanol, imidazolidinyl urea, chlorphenesin and the like may be used.

Carnauba wax, candenilla wax, beeswax, braze and the like may be used as the wax.

As the pH adjusting agent, triethanolamine, citric acid, or the like may be used.

As the fragrance, natural fragrance, combination fragrance, or the like may be used.

The formulation of the cosmetic prepared with the composition of the present invention is not particularly limited, and may have, for example, a formulation such as a nourishing cream, astringent lotion, a flexible lotion, a lotion, an essence, a nourishing gel or a massage cream.

In addition, the composition containing lactic acid bacteria crushing liquid according to the present invention can be used as a medicament for skin whitening and improvement containing lactic acid bacteria crushing solution that inhibits melanin synthesis as an active ingredient.

The medicament may be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, such as aerosols, external preparations such as powders, gels, ointments, creams, or sterile injectable solutions according to conventional methods. Can be used.

Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and such solid preparations include at least one excipient such as starch, calcium carbonate, sucrose in the composition. ), Or lactose, gelatin and the like can be mixed. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. . Preparations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, and suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate and the like can be used.

When the composition for skin whitening containing lactic acid bacteria crushing liquid according to the present invention is used as a medicament, the composition may further include a carrier, an excipient or a diluent. The carrier, excipient or diluent may include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline Cellulose, polyvinyl pyridone, water, methylhydroxybenzoate, propylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used.

When using the composition for skin whitening containing lactic acid bacterium crushing solution according to the present invention as a medicament, the preferred dosage depends on the condition and weight of the patient, the extent of the disease, the form of the drug, the route of administration and the duration of time, Can be selected. However, for the desired effect, the composition of the present invention is preferably administered at 0.0001 to 100 mg / kg per day. Administration may be administered once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect.

According to the present invention, the pharmaceutical composition for skin whitening containing lactic acid bacterium crushing solution may be administered to various mammals such as mice, mice, livestock, humans, and the like. All modes of administration can be expected, for example by oral, intravenous, intramuscular or subcutaneous injection.

According to the present invention, the pharmaceutical composition for skin whitening containing lactic acid bacteria pulverizing solution may further include a composition having a known skin whitening and improving effect.

In addition, the composition for skin whitening containing lactic acid bacteria crushing liquid according to the present invention can be used as a health food.

Examples of the health food to which the composition for skin whitening containing the lactic acid bacterium crushing liquid according to the present invention can be added include various foods, beverages, gums, teas, vitamin complexes, and health functional foods.

The amount of the skin whitening composition in the health food can be added in 0.01 to 15% by weight of the total food weight, the amount of the skin whitening composition in the beverage is 0.02 to 5 g, preferably 0.3 to 1 g based on 100 ml It can be added at the ratio of.

When using the composition for skin whitening containing lactic acid bacterium crushing liquid according to the present invention as a health food, there are no special limitations to other ingredients except for containing the composition as an essential ingredient in the indicated ratios, and various conventional flavoring agents or natural carbohydrates. Etc. can be contained as an additional component.

Examples of the natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of natural carbohydrates is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and its Salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. The compositions of the present invention may also contain pulp for the production of natural fruit juices and fruit juice beverages and vegetable beverages. These components can be used independently or in combination. The proportion of such additives is not so critical but it is generally chosen that the composition of the invention is selected in the range of 0 to about 20 parts by weight per 100 parts by weight.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, the following examples are illustrative of the present invention, and the contents of the present invention are not limited by the examples.

[ Production Example  1] Lactic acid bacteria crushing liquid manufacturing method

In order to prepare a culture solution, 5 ml of Lactobacillus MRS (Difco Co., Ltd.) liquid medium, a lactic acid bacteria growth medium, was placed in a 15 ml glass test tube, and then sterilized for 15 minutes at 121 ° C. Cultured in MRS agar medium to sterile medium seven kinds of lactic acid bacteria, Lactobacillus momentum spread (Lactobacillus of fermentum ), Enterococcus faecalis ), Leuconostoc citreum ), Lactococcus lactis ), Lactobacillus sakei , Streptococus mutans ) and Weisella cibaria were inoculated in colony form, respectively. The solution incubated for 24 hours in a 30 ℃ thermostat in the presence of oxygen was inoculated 2% (v / v) in the same sterile medium, and then incubated in the same conditions as the previous step. Finally, 50 ml of Lactobacillus MRS liquid medium was placed in a 100 ml Erlenmeyer flask to sterilize under the same conditions as above, and inoculated at 2% (v / v) in the medium, in the presence of oxygen. Culture was obtained by incubating for 48 hours at 30 ℃ thermostat.

To be plated thereof lactic acid bacteria in the decimal dilution method Lactobacillus bacteria (Lactobacillus) MRS agar medium check result, the seven types of lactic acid bacteria was ^{2 ~ 4 X 10 9 CFU /} ㎖ 30 bungan centrifuged at 8,000rpm at 4 ℃ the culture 50㎖ The cells were separated to obtain cells.

After washing twice with sterile water. 10 ml of sterile water was added and the cells were disrupted under conditions of 40% amplitude, pulse on 20 sec, off 10 sec, 15 ° C., and 25 minutes (Vibracell, SONICS).

The debris obtained here was centrifuged once again in the same manner as above to remove cell debris and the supernatant was obtained as a final lactic acid bacteria debris.

At this time, the components of the cell precipitate are mostly hydrophobic cell wall components, the components of the supernatant are components of the lactic acid bacteria cells, peptidoglycan, cell membrane components, intracellular proteins, various kinds of intracellular enzymes and the like.

[ Example  1] Inhibitory effect of melanin synthesis in melanocyte tumor cells

In order to determine the melanin synthesis inhibitory effect in the melanocyte tumor cells of the lactic acid bacteria lysate of the present invention was performed as follows. First, the mouse-derived malignant melanin tumor cell line B16F1 (KCLB 8007) was added to DMEM (Dulbecco's Modified Eagle's Medium) medium containing penicillin (100 IU / mL), streptomycin (100 g / mL), and 10% FBS (fetal bovine serum). In 37 ℃ and maintained in the incubator containing 5% carbon dioxide. The obtained melanin tumor cells were seeded in 1 × 10 ⁴ cells / well in a 24-well plate and cultured for 1 day. After incubation, the medium was removed, and then, in order to induce melanin synthesis, melanin synthesis was induced by adding 50 nM of alpha-MSH (melanocyte stimulating hormone) except for the non-treated group, and simultaneously crushing 7 kinds of lactic acid bacteria. Liquid ( Lactobacillus fermentum ), Enterococcus faecalis ), Leuconostoc citreum ), Lactococcus lactis , Lactobacillus sakei ), Streptococus mutans ) and Weisella cibaria were incubated for 3 days with DMEM medium added 0.5% of kojic acid (positive control) 800uM, respectively. The amount of synthesized melanin secreted by melanin tumor cells by each treatment group was visually confirmed, and the change was photographed by a digital camera, and is shown in FIG. 2.

In the photograph of FIG. 2, the medium color is increased to brown according to the degree of melanin synthesis. In the alpha-MSH alone group, the melanin synthesis is significantly increased by alpha-MSH treatment, and the medium color is higher than the non-treated group. The color became dark brown, and the medium color of the lactic acid bacteria lysate treated group treated with alpha-MSH was similar to that of the non-treated group. As a result, it was confirmed that the lactic acid bacteria lysate acts strongly to inhibit melanin synthesis induced by alpha-MSH.

[ Example  2] mushroom Tyrosinase  Activity inhibitory effect

In order to determine the inhibitory effect of the mushroom tyrosinase activity of the lactic acid bacteria lysate of the present invention, the following experiment was performed.

First, mushroom tyrosinase (Sigma; T3824) was prepared by dissolving at 10ug / ml concentration in 10mM Sodium phosphate buffer (SPB). In addition, L-DOPA (3,4-dihydroxy-L-phenylalanine, Sigma; D9628-5G) was prepared by melting 10mM SPB in 10mM concentration. 100 μl of 10 mM SPB was added to a 1.5 ml Eppendorf tube, and samples were added at a multiple of concentration up to 0.5-16 % of lactic acid bacteria lysate of 200 uM kojic acid and Lactococcus lactis . Then, 10 ug / ml mushroom tyrosinase was added to each tube by 80ul, and 10mM L-DOPA was added to each tube by 20ul and shaked to mix gently. A 96-well plate was prepared and 90 ul of the reaction solution in the tube was added to each well to change the color, and the absorbance was measured by 475 nm.

Figure 3 shows the change in absorbance according to the color change of the degree of inhibition of mushroom tyrosinase activity by lactic acid bacteria lysate of Lactococcus lactis, Lactobacillus lysate showed significant inhibitory activity of mushroom tyrosinase activity It was confirmed that it has.

[ Example  3] Tyrosinase  Expression inhibitory effect

In order to determine the inhibitory effect of tyrosinase expression of the lactic acid bacteria lysate of the present invention, the following experiment was performed.

First, the mouse-derived malignant melanin tumor cell line B16F1 (KCLB 8007) was converted to DMEM (Dulbecco's Modified) containing penicillin (100 IU / mL), streptomycin (100 g / mL; Hyclone), and 10% FBS (fetal bovine serum; Hyclone). Eagle's Medium (Hyclone) medium was maintained in 37 ℃ and incubated in an incubator containing 5% carbon dioxide. The obtained melanin tumor cells were seeded in 1 × 10 ⁵ cells / well in 6 well plates and cultured for 1 day. After incubation, the medium was removed, and then, in order to induce melanin synthesis, melanin synthesis was induced by adding 50 nM of alpha-MSH (melanocyte stimulating hormone) except for the non-treated group, and simultaneously crushing 7 kinds of lactic acid bacteria. Liquid ( Lactobacillus fermentum ), Enterococcus faecalis ), Leuconostoc citreum ), Lactococcus lactis , Lactobacillus sakei ), Streptococus mutans ) and Weisella cibaria were incubated for one day with the addition of 0.5% and 1% DMEM medium, respectively. After collecting the cells of each treatment group, lysates were obtained by lysing the cells with SDS cell lysis buffer (addition of 2% SDS), and total protein amount was quantified by Bradford method. Western sample was prepared by correcting the protein amount. The prepared samples were separated according to size in 10% SDS-PAGE, and then the proteins transferred to PVDF membranes were visualized on an X-ray film using an anti-tyrosinase antibody as a primary antibody.

Figure 4 confirmed that the degree of tyrosinase expression induced by alpha-MSH was inhibited by lactic acid bacteria lysate, it was confirmed that the lactic acid bacteria lysate strongly inhibited tyrosinase expression.

[ Example  4] Cell safety test of lactic acid bacteria lysate

In order to determine the cell stability of the lactic acid bacteria lysate of the present invention, the following experiment was performed.

First, the mouse-derived malignant melanin tumor cell line B16F1 (KCLB 8007) was converted to DMEM (Dulbecco's Modified) containing penicillin (100 IU / mL), streptomycin (100 g / mL; Hyclone), and 10% FBS (fetal bovine serum; Hyclone). Eagle's Medium (Hyclone) medium was maintained in 37 ℃ and incubated in an incubator containing 5% carbon dioxide. The obtained melanin tumor cells were seeded in 1 × 10 ⁴ cells / well in 96 well plates and cultured for 1 day. After cultivation, the medium was removed, and then lactic acid bacteria lysate was added to the DMEM medium containing 0.1% and 0.5%, respectively, and cultured for 1 day. 50 μl of MTT (3- [4,5-dimethylthiazol-2-yl] -2,5-diphenyl-tetrazolium bromide, Sigma) solution dissolved in 1xPBS at 1mg / ml concentration was added to each treatment group. Incubated for 2 hours. Thereafter, the contained MTT solution was removed, and formazan crystals were formed by adding 100ul of DMSO, respectively, and the absorbance at 570nm was measured using a microplatereader machine.

5 is confirmed by the MTT assay whether the lactic acid bacteria lysate is safe for use in cells, the cell viability shown in the non-treated group and the cell viability shown in each lactic acid bacteria treated group is about 100%, There was no cell damage. By the above experiment, it was found that the lactic acid bacteria lysate was a safe substance for the cells.

[ Production Example  1] containing lactic acid bacteria lysate Cosmetics

A. Cream containing lactic acid bacteria crushed liquid

The preparation example of the nutrient cream containing lactic acid bacteria crushing liquid is shown in Table 1 below. The aqueous phase purified water, triethanol amine and propylene glycol were heated and dissolved at 70 degreeC, and the oily fatty acid, an oily component, an emulsifier, and the preservative were heated to 70 degreeC, and the liquid which melt | dissolved was added and emulsified. After emulsification was completed, the solution was cooled to 45 ° C., lactic acid bacteria crushed solution and flavor were added, dispersed, and cooled to 30 ° C.

ingredient Content (% by weight) Lactobacillus crushed liquid 0.1% or 0.5 or 1.0% Jojoba oil 5.0 Floating paraffin 7.0 Cetylaryl alcohol 2.0 Polyglyceryl-3 Methyl Glucose Disteare 2.0 Glyceryl Stearate 0.5 Squalane 3.0 Propylene glycol 4.0 glycerin 5.0 Triethanol amine 0.3 Carboxy Vinyl Polymer 0.3 Tocopheryl acetate 0.2 Preservative, incense a very small amount Purified water Remaining amount Sum 100

B. Lotion with Lactobacillus Crushing Liquid

a) flexible lotion

Examples of the preparation of the flexible lotion containing lactic acid bacteria crushing liquid are shown in Table 2 below. Purified water was added to the aqueous component and dissolved at room temperature to prepare an aqueous phase. The lactic acid bacteria crushing liquid, oily component, emulsifier, preservative, and fragrance were dissolved at room temperature, added to the aqueous phase, mixed, and filtered.

ingredient Content (% by weight) Lactobacillus crushed liquid 0.10 EDTA 0.05 Fiji 1500 4.00 Carbomer 0.16 1,3 Butylene Glycol 3.00 Polyoxyethylene Hydrogenated Soap Castor Oil 0.45 Triethanolamine 0.12 Preservative, fragrance, coloring a very small amount Purified water Remaining amount Sum 100

b) converging lotion

A preparation example of a convergent lotion containing lactic acid bacteria crushing liquid is shown in Table 3 below. Purified water was added to the aqueous component and dissolved at room temperature to prepare an aqueous phase. Ethanol was added to the lactic acid bacteria pulverizing solution, oil phase component, emulsifier, preservative, and fragrance to dissolve at room temperature to prepare an alcohol phase, which was then added to the aqueous phase, mixed, and filtered.

ingredient Content (% by weight) Lactobacillus crushed liquid 0.10 glycerin 2.00 1,3 butylene glycol 3.00 EDTA 0.05 ethanol 7.00 Polyoxyethylene Hydrogenated Castor Oil 0.40 Preservative, fragrance, coloring a very small amount Purified water Remaining amount Sum 100

C. Lotion

The preparation example of the lotion containing the lactic acid bacteria crushing liquid is shown in Table 4 below. The aqueous phase purified water, triethanol amine, and butylene glycol were dissolved by heating to 70 占 폚, and the oily fatty acid, oily component, emulsifier, and preservative were heated to 70 占 폚 to dissolve, and then emulsified. After the emulsification was completed, a 2% solution of xanthan gum, a hydrophilic thickener, was added to adjust the amount to 0.05% by weight. After cooling the solution to 45 ℃, lactic acid bacteria crushing liquid, flavor and pigment was added and mixed and then cooled to 30 ℃.

ingredient Content (% by weight) Lactobacillus crushed liquid 0.10 glycerin 3.00 Carbomer 0.10 Xanthan Gum 0.05 1,3 Butylene Glycol 3.00 Polyglycerin-3 Methylglucose Distearate 1.50 Glycerin Stearate 0.50 Cetearyl Alcohol 0.30 Jojoba oil 3.00 Floating paraffin 2.00 Squalane 3.00 Dimethicone 0.50 Tocopheryl Acetate 0.20 Triethanolamine 0.10 Preservative, fragrance, coloring a very small amount Purified water Remaining amount Sum 100

D. gel

Preparation of the gel containing the lactic acid bacteria crushing liquid is shown in Table 5 below.

ingredient Content (% by weight) Lactobacillus crushed liquid 0.10 EDTA 0.02 1,3 Butylene Glycol 4.00 Carbomer 0.60 glycerin 5.00 Hydroxyethyl cellulose 0.15 Triethanol amine 0.50 Triclosan 0.20 ethanol 5.00 Polyoxy Ethylene Hydrogenated Castor Oil 4.00 Preservative, fragrance, coloring a very small amount Purified water a very small amount Sum 100

E. Essence

Preparation of the essence containing the lactic acid bacteria crushing liquid is shown in Table 6 below. Thickener was added to the purified water with slow stirring to disperse uniformly. The aqueous phase was mixed to prepare the aqueous phase. Ethanol was added to the emulsifier, skin softener, fragrance, and preservative to dissolve. The alcohol phase was added to the aqueous phase with stirring and an alkali agent was added. Finally, lactic acid bacteria crushing liquid and a pigment were added.

ingredient Content (% by weight) Lactobacillus crushed liquid 0.10 EDTA 0.05 Potassium-cetylphosphate 0.30 glycerin 5.00 1,3 Butylene Glycol 4.00 Glyceryl Stearate / Pig-100 Stearate 0.50 Sorbitan stearate 0.30 Jojoba oil 0.50 Macadamia Nut Oil 0.50 Tocopheryl Acetate 0.50 Butylated hydroxy toluene 0.05 Dimethicone 3.00 Polyacrylamide / C13-14 Isoparaffin / Laureth-7 1.50 Glyceryl Polymethacrylate / Propylene Glycol 5.00 ethanol 3.00 Preservative, fragrance, coloring a very small amount Purified water Remaining amount Sum 100

[compare Production Example  One] Purified water  Cream containing

Cream was prepared by the same method and formulation as in Preparation Example 1-A, except that lactic acid bacteria lysate was added and purified water was replaced.

[ Production Example  2] of health food containing lactic acid bacteria crushed liquid Production Example

The health food composition containing the lactic acid bacteria crushing liquid was prepared by a conventional liquid preparation method with the same composition as the respective preparation examples. The final volume is 100 ml in each liquid. This formulation example can be prepared by a conventional production method as well as liquid tablets, powders, granules and the like.

ingredient content Lactobacillus crushed liquid 100 mg honey 1500 mg Vitamin c 50 mg Vitamin B ₆ 10 mg Nicotinic acid amide 10 mg Royal jelly 80 mg Preservative, fragrance, coloring a very small amount Purified water Remaining amount Sum 100 mg

[ Production Example  3] Preparation of Lactic Acid Bacteria Shredding Solution-Containing Drug

The lactic acid bacterium crushing solution of the present invention is mixed with excipients or adjuvants commonly used in pharmacy daily, and formulated into pharmaceutical preparations which can be administered orally or parenterally by conventional pharmaceutical methods to be used as pharmaceuticals. Can be. Next, the preparation example of a preparation is illustrated as a preparation example.

Formulation Example  A: Preparation of Tablet

Lactobacillus crushed solution 5mg

Lactose 20mg

Starch 20mg

Magnesium stearate proper amount

The above ingredients are mixed and compressed into 50 mg tablets according to a conventional method for producing tablets.

Formulation Example  B: Preparation of Capsule

Lactobacillus crushed solution 5mg

Lactose 20mg

Starch 19mg

Talc 1mg

Magnesium stearate proper amount

The above ingredients are mixed and filled into 50 mg of gelatin capsules according to a conventional method for producing a capsule.

Formulation Example  C: Preparation of Injection

Lactobacillus crushed solution 5mg

Melting aid amount

Appropriate sterile distilled water for injection

The above ingredients are usually filled in 2 ml ampoules according to the preparation method of the injection, sealed and sterilized to prepare the injection.

Formulation Example  D: Preparation of Syrup

Lactobacillus crushed solution 5mg

틘 80 drops

5 g of sugar

5g of isomerized sugar

Purified water

50 ml total

The above ingredients are added to purified water, stirred well, and then filled into 50 ml glass bottles and sterilized to prepare a syrup.

Formulation Example  E: Preparation of Ointment

Lactobacillus crushed solution 5mg

Diethanolamine 1.5g

5 g of polyvinylpyrrolidone

Propylene Glycol 30g

Add 100 ml of distilled water

The ointment is prepared according to the above-described method for producing an ointment.

As described above, the composition containing the lactic acid bacteria lysate of the present invention inhibits melanin synthesis, and has a whitening or improving effect on the skin, and thus may be used as a new cosmetic, pharmaceutical and health food.

Claims (12)

Skin whitening composition containing lactic acid bacteria crushing liquid. The method of claim 1, wherein the lactic acid bacteria lysate is Lactococcus sp . ), Streptococcus sp . ), Weisella sp . ), Lactobacillus sp . ), Enterococcus sp . ) And Leuconostoc sp .) After obtaining the cells by culturing the lactic acid bacteria selected from the group consisting of the composition for skin whitening. The composition for skin whitening according to claim 2, wherein the lactic acid bacteria is cultured in the presence of oxygen. According to claim 2, The lactic acid bacteria Lactobacillus fermentum ( Lactobacillus fermentum ), Enterococcus faecalis ( Enterococcus) faecalis ), Leuconostoc citreum ), Lactococcus lactis ), Lactobacillus sakei ), Streptococus mutans ), Weisella cibaria ( Weisella cibaria ) is selected from the group consisting of skin whitening composition. The composition for skin whitening according to claim 1, wherein the lactic acid bacteria lysate is included in an amount of 0.1 to 1.0% by weight based on the total weight of the composition. The composition for skin whitening according to claim 1, wherein the lactic acid bacterium crushing solution inhibits melanin synthesis. The composition for skin whitening according to any one of claims 1 to 6, which is used as an external preparation for skin. The composition of claim 7, wherein the external preparation for skin has a formulation selected from the group consisting of powder, gel, ointment, cream, liquid, and aerosol. The composition for skin whitening according to any one of claims 1 to 6, which is used as a cosmetic. The method of claim 9, wherein the cosmetic cream has a formulation selected from the group consisting of a nourishing cream, astringent lotion, a flexible lotion, lotion, essence, nutrition gel, lipstick, foundation, eye shadow, patch, mast sheet and massage cream Skin whitening composition. The skin whitening composition according to any one of claims 1 to 6, which is used as an active ingredient of a skin whitening agent. The composition for skin whitening according to any one of claims 1 to 6, which is used as a health food.

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