KR20070015895A - Recovery device for deoxyribohexane and ribohexane or protein fragments using electrophoresis on agarose gel or polyacrylamide gel - Google Patents

Abstract

After electrophoresis of DNA, RNA or protein in Agarose Gel or Polyacrylamide Gel, separated and purified DNA, RNA or protein is recovered directly on Aarose Gel or on Polyacrylamide Gel.

The present invention improves conventional methods of recovering DNA, RNA or protein fragments in a gel by fragmenting the gel to recover purified DNA, RNA or protein fragments by electrophoresis of DNA, RNA or protein. .

Electrophoresis on Agarose Gel or Polyacrylamide Gel, followed by electrophoresis of DNA, RNA or Protein Fragments, or electrophoresis once more to recover DNA by sending DNA, RNA or Protein to the desired location. Integrates the extraction process of DNA and RNA protein in the direction or the reverse direction, and extracts DNA, RNA or Protein from the electrophoretic system different from the conventional method.

DNA, RNA, Protein, Agarose, Polyacrylamide, extract in gel, DNA and RNA furified, Fragments, Electrophoresis.

Description

Recovery system of DNA and RNA or protein fragments with agarose gel or Polyacrylamide gel} using electrophoresis on agarose gel or polyacrylamide gel

1 is a gel plate of the present invention.

Figure 2a is a form for supplying power to the gel plate of the present invention.

Figure 2b is a rear portion of the electrode for supplying power to the gel plate and there is an electric circuit on the back is a view away from the electrode.

Figure 3 is a gel plate of the present invention.

4 shows a sequence of crossing the wells when the fragments have wells.

5 is an image of the state in which the conductor and the non-conductor is placed during the electrophoresis.

Figures 6a, 6b is the most basic form of Fragments recovery device in the present invention and shows the principle.

7 is a device for recovering the fragments more effectively than in FIG.

8 is a device for recovering more effectively than FIG.

9 illustrates the distance between the Gel surface and the Well and explains the X-axis direction.

Figure 10 shows the principle of not being contaminated from the side lanes when recovering large quantities of fragments at the same time.

Figure 11 shows an overview of an apparatus for simultaneously recovering large quantities of fragments simultaneously without being contaminated from the side lane.

12 is a device capable of recovering Fragments of any size from the same sample in a manner of simultaneously recovering a large number of Fragments.

Figure 13 is a device that can be easily used to make a large amount of recovery device.

<Description of the symbols for the main parts of the drawings>

11: Thin film 12: Slit 13: Gel cast and Gel Pate 14: Gel

16: Well into which the sample to be purified and recovered such as DNA, RNA, protein, and tissue is injected

31,32,41,42,51,52,71,72,81,111,112: current supply electrode or wire

44: Well containing buffer or electrolyte

45: DNA, RNA or Protein Fragments

53: insulator

55: conductor

62,63,66: plate consisting of conductors

65: insulated thin plate

53: insulator

55: conductor

62,63,66: plate consisting of conductors

65: insulated thin plate

74: A thin film well consisting of two plates with some conductors and insulators

92: Thickness of well and gel outer wall into which sample is injected.

103: Thinner than 92 thicknesses to avoid contamination from side lanes

113,114,115: Subdivide the electrophoretic section with the X, Y, and Z axes to facilitate theoretical explanations.

The present invention invents one of the techniques that can be used to separate DNA and RNA or proteins from Agarose gel or Polyacrylamide Gel. The present invention is one of several methods for recovering DNA fragments or RNA fragments or protein fragments present in the gel after electrophoresis in Agarose Gel or Polyacrylamide Gel (hereinafter referred to as Gel).

Electrophoresis in Agarose Gel is mostly for DNA or RNA. In polyacrylamide Gel, electrophoresis is usually done for proteins, but sometimes electrophoresis for DNA or RNA.

In the present invention, after electrophoresis on Agarose Gel and Polyacrylamide Gel, the thin films 11 having a width of DNA, RNA or Protein Fragments are arranged at a narrow interval on the gel. The thin film is made of slit (12) and fills the buffer or electrolyte with the slit, and then places the electrophoretic electrodes 21 (+,-) on the buffer or electrolyte. At this time, the electrode is usually plugged into the + electrode 21, but-depending on the charge of the fragments to be recovered-may be plugged in. Here it is shown to use the electrode 21 attached to the cover of the electrophoretic tank (Fig. 2). The electrode (Fig. 2a) attached to the lid (Fig. 2) is contained in the slits together with a buffer or an electrolyte. After the electrophoresis on the gel (14), the power (31, 32) in the direction in which the Flagments proceeded is cut off and the power in the direction in which the Flagments proceeded to the electrode 21 on the cover (FIG. 2B) to send, Slit ( 12) DNA, RNA or protein fragments flow into the buffer or electrolyte in the compartment. Buffer or electrolytic material is then recovered to recover DNA, RNA or protein fragments.

The slit 12 is perforated in the lower part 14, where the lower part refers to the upper part 15 of the Agarose Gel or the one side 93 of the Polyacrylamide Gel which is in contact with the Slit. Slit (12) is composed of thin film fragments (FIG. 6), and in order to extract DNA, RNA or protein, it must have at least one surface like a thin film (FIG. 6). A buffer or an electrolytic material is supplied to the back surface 61, and the electrophoretic power supply 62 enters the supplied buffer or electrolytic material.

One side, such as a slit or thin film, is placed on top of the gel or inside the gel. A slit made up of slit, or one slit or more than one slit, allows DNA, RNA For example, let the Potein Flagments gather in the Slit. Therefore, it is different from the conventional method in which DNA, RNA, and Potein Flagments can be recovered only by gel fragmentation.

There are several known ways to extract proteins from DNA and polyacryamide in Agarose Gel. The commonalities between these methods are that the gel must be sectioned after electrophoresis and the electrophoresis must be stopped to section.

Usually, after electrophoresis, DNA, RNA or protein fragments in the gel are cut into gel pieces. Each bend is sectioned to increase the selectivity of the desired flags. There are several ways to recover DNA, RNA or protein from the fragmented gel.

Commonly used method is to place the gel in the Dialysis Tube, fill the appropriate buffer, and then electrophoresis in the electrophoresis tank. Then the Fragments in the Gel are ejected and the contents in the Tube are recovered except the Gel. Recover the fragments to be obtained by separating the recovered buffer solution and fragments.

Alternatively, dissolve the gel by applying the medicine or heat to the sliced gel, and then insert the fine glass binder, magnetic material or resin directly into the fragments. And recover it by recovering it directly by separating the bonded glass Bid or magnetic micro resin.

DNA or RNA fragments are recovered in more ways than proteins. Place the Membrane in the syringe cylinder and place the sliced Agarose Gel in the syringe cylinder. Then push the syringe piston with the appropriate force to crush the Gel. Push the piston to the end in the crushed state and recover the DNA attached to the membrane.

Add one or two drops of Buffer solution onto the sliced gel. Then, with the gel in between, the cathode and anode wires are placed on both sides, and the electric charge is transmitted to the buffer solution and the gel. The DNA in the gel is then pushed into the buffer solution. Thus, the DNA contained in the buffer solution is extracted.

 Drill both sides of the plastic tube with the lid and place a Membrane on it. In this state, place the sliced gel in the tube, close the lid, and let the membrane and the gel line up. The tube is then placed in an electrophoretic tank to allow charge to pass through the membranes on both sides, allowing DNA, RNA or protein in the gel to be extracted. After a certain period of time, when the DNA, RNA or protein is removed from the gel, turn the tube upside down to reverse the DNA, RNA or protein from the flow of charge and remove the DNA, RNA or protein from the membrane. . It takes a buffer in the tube and retrieves the fragments in it.

There are various techniques described above, and the most commonly used technique is a form using a filter column. Place filter paper with glass components in the column. The gel is sliced and the gel is dissolved using a chemical, and the dissolved solution is passed through the filter column. Then, Fragments should be combined with the glass component of the filter paper and washed to recover the DNA. Protein extracts protein using many resin-only methods. This method is the most widely used method in recent years, and the detailed technology of this method is evolving rapidly, which is a big axis of the bioscience industry.

 The present invention is a method for extracting DNA, RNA or protein on the gel, in order to extract the fragments without separating the gel fragments, there must be an auxiliary device of the gel plate or gel plate designed in a special form. In order to use this gel plate, the electrophoretic tank and electric supply device must be designed differently from the existing one. In addition, the function of the electrophoretic tank must be added separately and designed for such functions in order to easily distinguish each fragment and to accomplish the work in one step without stopping the work.

The present invention invents one of the techniques that can be used to separate DNA and RNA or proteins from Agarose gel or Polyacrylamide Gel. The present invention is one of several methods for recovering DNA fragments or RNA fragments or protein fragments present in the gel after electrophoresis in Agarose Gel or Polyacrylamide Gel (hereinafter referred to as Gel).

Electrophoresis in Agarose Gel is mostly for DNA or RNA. In polyacrylamide Gel, electrophoresis is usually done for proteins, but sometimes electrophoresis for DNA or RNA.

In the present invention, after electrophoresis on Agarose Gel and Polyacrylamide Gel, the thin films 11 having a width of DNA, RNA or Protein Fragments are arranged at a narrow interval on the gel. The thin film is made of a slit (12), and then filled with a buffer or electrolyte in the slit, and the electrophoretic electrodes 21 (+,-) are inserted into the buffer or the upper layer of the electrolyte. At this time, the electrode is usually plugged into the + electrode 21, but-depending on the charge of the fragments to be recovered-may be plugged in. Here it is shown to use the electrode 21 attached to the cover of the electrophoretic tank (Fig. 2). The electrode (Fig. 2a) attached to the lid (Fig. 2) is contained in the slits together with a buffer or an electrolyte. After the electrophoresis on the gel (14), the power (31, 32) in the direction in which the Flagments proceeded is cut off and the power in the direction in which the Flagments proceeded to the electrode 21 on the cover (FIG. 2B) to send, Slit ( 12) DNA, RNA or protein fragments flow into the buffer or electrolyte in the compartment. Buffer or electrolytic material is then recovered to recover DNA, RNA or protein fragments.

The slit 12 is perforated in the lower part 14, where the lower part refers to the upper part 15 of the Agarose Gel or the one side 93 of the Polyacrylamide Gel which is in contact with the Slit. Slit (12) is composed of thin film fragments (FIG. 6), and in order to extract DNA, RNA or protein, it must have at least one surface like a thin film (FIG. 6). A buffer or an electrolytic material is supplied to the back surface 61, and the electrophoretic power supply 62 enters the supplied buffer or electrolytic material.

One side, such as a slit or thin film, is placed on top of the gel or inside the gel. A slit made up of slit, or one slit or more than one slit, allows DNA, RNA For example, let the Potein Flagments gather in the Slit. Therefore, it is different from the conventional method in which DNA, RNA, and Potein Flagments can be recovered only by gel fragmentation.

There are several known ways to extract proteins from DNA and polyacryamide in Agarose Gel. The commonalities between these methods are that the gel must be sectioned after electrophoresis and the electrophoresis must be stopped to section.

Usually, after electrophoresis, DNA, RNA or protein fragments in the gel are cut into gel pieces. Each bend is sectioned to increase the selectivity of the desired flags. There are several ways to recover DNA, RNA or protein from the fragmented gel.

Commonly used method is to place the gel in the Dialysis Tube, fill the appropriate buffer, and then electrophoresis in the electrophoresis tank. Then the Fragments in the Gel are ejected and the contents in the Tube are recovered except the Gel. Recover the fragments to be obtained by separating the recovered buffer solution and fragments.

Alternatively, dissolve the gel by applying the medicine or heat to the sliced gel, and then insert the fine glass binder, magnetic material or resin directly into the fragments. And recover it by recovering it directly by separating the bonded glass Bid or magnetic micro resin.

DNA or RNA fragments are recovered in more ways than proteins. Place the Membrane in the syringe cylinder and place the sliced Agarose Gel in the syringe cylinder. Then push the syringe piston with the appropriate force to crush the Gel. Push the piston to the end in the crushed state and recover the DNA attached to the membrane.

Add one or two drops of Buffer solution onto the sliced gel. Then, with the gel in between, the cathode and anode wires are placed on both sides, and the electric charge is transmitted to the buffer solution and the gel. The DNA in the gel is then pushed into the buffer solution. Thus, the DNA contained in the buffer solution is extracted.

 Drill both sides of the plastic tube with the lid and place a Membrane on it. In this state, place the sliced gel in the tube, close the lid, and let the membrane and the gel line up. The tube is then placed in an electrophoretic tank to allow charge to pass through the membranes on both sides, allowing DNA, RNA or protein in the gel to be extracted. After a certain period of time, when the DNA, RNA or protein is removed from the gel, turn the tube upside down to reverse the DNA, RNA or protein from the flow of charge and remove the DNA, RNA or protein from the membrane. . It takes a buffer in the tube and retrieves the fragments in it.

There are various techniques described above, and the most commonly used technique is a form using a filter column. Place filter paper with glass components in the column. The gel is sliced and the gel is dissolved using a chemical, and the dissolved solution is passed through the filter column. Then, Fragments should be combined with the glass component of the filter paper and washed to recover the DNA. Protein extracts protein using many resin-only methods. This method is the most widely used method in recent years, and the detailed technology of this method is evolving rapidly, which is a big axis of the bioscience industry.

 The present invention is a method for extracting DNA, RNA or protein on the gel, in order to extract the fragments without separating the gel fragments, there must be an auxiliary device of the gel plate or gel plate designed in a special form. In order to use this gel plate, the electrophoretic tank and electric supply device must be designed differently from the existing one. In addition, the function of the electrophoretic tank must be added separately and designed for such functions in order to easily distinguish each fragment and to accomplish the work in one step without stopping the work.

The ultimate goal of the life science industry is to prolong quality of life. The direction of this goal has evolved in many different fields and forms. Molecular biology, among other things, is taking very slow steps ahead of many easily imaginable assignments. The discovery of the most basic DNA, the more advanced RNA, the protein close to the end point, and the research from the DNA field to the protein are being conducted sequentially and simultaneously.

 This work consists of different fragmentary works. These are different paths of work, and several tasks are selected and combined as needed, resulting in short plot work. Most of these works are done by hand and must be made up of highly educated people due to the nature of the work. For these reasons, the pace of research is much slower than scientists think. Therefore, it would be ideal for scientists to be able to work as fast as their brains. This necessity necessitated a technique called automation, and in the study of DNA automation was done in sequencing. But in the previous stages of sequencing, it still consists of fragmentary works.

With this in mind, this technology has solved the technical solution for automation, which will be a chance to realize time-saving and high reproducibility and to solve technical clues for future automation.

The present invention can be easily understood by contemplating the eight experimental forms that have been performed for the same purpose in order. The following experimental conditions are given in the context of general electrophoresis for the isolation and identification of DNA, RNA and protein. The following conditions were based on Horizontal. Vertical is the same condition, and the same results are achieved just by converting the horizontal to vertical position.

First, when electrophoresis is performed on Agarose Gel or Polyacrylamide Gel (Fig. 4), generally Fragments flow from the-(41) electrode direction to the + electrode 42 direction. In the gel plate (43), create a well (44) in the direction the fragments flow and fill the buffer with a well (44). Electrophoresis of this, if the Fragments (45) of each type touches the well quickly cross (48) across the well. Even if fluorescent materials or dyes are attached to the fragments to emit light, the direct view across the wells (48) cannot be observed with the naked eye. At this time, it can be seen that various fragments 47 disappearing in the −electrode direction with the well 44 therebetween appear in the + electrode direction. At this time, if the current is cut off and the buffer in the well 49 is recovered, a small amount of various fragments can be detected.

Here, it is very efficient to put DNA, RNA or protein fragments into the buffer or electrolyte, that is, fine glass binder, magnetic material or resin, and take them out together.

Second, when electrophoresis is performed on Agarose Gel or Polyacrylamide, if non-conducting material is inserted on Gel (53) between-(51) and + (52) power, the electrophoresis is not performed as much as the width of insulator 53. A section of 54 occurs. At this time, if the conductor 53 which flows very well instead of the non-conductor is plugged in, electrophoresis 57 is performed in the direction of the -electrode from the conductor. Electrophoresis until the fragments reach the + power supply will cause a shadow (57) on the + power side from the conductor.

Thirdly, the contents of the second experiment were combined to form a thin film (65) by combining the conductor and the non-conductor, and forming the conductor (62) on the + electrode side and the non-conductor (61) on the electrode side. Then, the conductor 63 was transferred to the upper portion 66 of the insulator 65 so as to be covered to an arbitrary height of the upper end of the insulator (62). Let's call it "Lamination" by any name. After electrophoresis on the gel, plug the "lamination film" (FIG. 6) into the + electrode direction of the Fragments to be recovered, that is, the + end of the bend at the Fragments bend. When this "lamination" is plugged into the Gel plate, the conductor should be directed towards the + electrode direction and the non-conductor towards the-electrode direction, and the gel should not touch the conductor 62 at the upper end of the insulator of this "lamination film". . Then, the electrolyte or the buffer is dropped in the insulator direction of the "layered film", that is, in the -electrode direction. The electrolyte or buffer solution should not be spread, but only on the bend to be extracted. The flow of charge then starts from the + electrode and through the conductor of the "layer" through the conductor 62 on top of the non-conductor portion of the "layer" via the electrolyte or buffer to the fragments. Fragments proceed in the opposite direction to the flow of charge and stop their movement in the electrolyte or buffer solution. And by pipetting Buffer, various fragments can be easily recovered. This experiment was able to recover the fragments on the gel.

Fourth, another test method is to make the "layer film" differently. In the "lamination film", a non-conductive thin film (73) is added to the side where the buffer solution or the electrolyte contacts, and a form in which the buffer solution or the electrolyte is securely confined is trapped (FIG. 7B and 7C). The "thin film" made in this way is called "layer thin film well", between the insulator thin film and the "layer thin film" is called "Slit" and this "Slit" also called "Well". After the electrophoresis, the "Slit" is raised to the upper portion 74 of the Fragments Band to be extracted. Insert "Layer Thin Film Well" (Figure 7b, Figure 7c) on the Gel and put the buffer solution or electrolytic material in the "Well" and electrophoresis again. The DNA or RNA fragments can then be recovered from the electrolyte or buffer solution in "Slit".

At this time, the lower portion of the "layered film" of the "layered film well" is inserted into the gel without cutting into the gel, and cut into the portion to be inserted (Fig. 8b). And remove only the conductor portion of the + electrode side and directly connect the + power supply 81 to the upper portion. Shut off the electrophoresis + power supply and supply current to the electrophoresis-power supply and the + power supply connected to the top of the "double layer well". DNA, RNA or Protein Fragments can then be collected in "Slit" with a Buffer or Electrolyte solution.

Fifth, as shown in Fig. 10, the conductors are changed in position to form a "layer thin film well". And the leg 101 is made in the lower portion, the partition 101 such as curtains are also made in the leg. Then place a "laminar membrane well" (FIG. 11) on the lane of the sample to be separated during electrophoresis.

If the fragments to be extracted on the gel are found just below the slit, stop the electrophoresis that is in progress and supply power to the power of the conductor connection and the correlated power. Then, the fragments flowing in the direction from-(111) to + (112) in the direction of the arbitrary X axis 113 are simultaneously moved in the directions of the arbitrary Y axis 114 and the arbitrary Z axis 115. At this time, the Y 114 axis refers to the direction perpendicular to the horizontal plane of the X (113) axis, and when the X axis and the Y axis in the plane, the vertical direction is Z (115) axis. Fragments that have been displaced are included in the buffer solution or electrolyte in this slit or well.

Here, in the "layer thin film well", the lower portion 116 in the Y-axis direction descends more than the lower portion in the X-117 axis direction. This prevents mixing from the fragments of the side lanes when electrophoresed on multiple lanes. This should be a height 92 from the well bottom 91 of the gel to the bottom of the gel, higher than the height of the bottom 103 of the Y axis 102 of the “lamination well”.

Sixth, the results of the above experiment shows that fragments such as DNA, RNA or protein can be partially recovered when electrophoresed. When electrophoresis to several lanes, it is necessary to have a device that can recover the fragments of the various bands needed at the same time. Thus, they are stacked in layers by adding them horizontally and vertically. The conductor of the thin film well is connected directly to the + power supply or the conductor is completely removed from the "layer thin film well", and the "pin" of the + electrode is directly immersed in the buffer solution or the electrolyte. When making an agarose or polyacrylamide gel, the well (94) position in the gel is in the direction of the X (95) axis coinciding with the thin film well or another name, Slit, and the bottom height (94) of the well (94) in the gel. Alternatively, the bottom position of the well 92 in the gel should be higher than the lower height in the direction of the Y axis 102 among the lower portions of the "layer thin film well". This eliminates the possibility of the accompanying lane's fragments coming along when extracting each fragment. Thus, the fragments are extracted by adding a buffer solution or an electrolyte to the slit and flowing a + current in the slit and a-current on the same axis.

Seventh, when recovering the Fragmenets after the electrophoresis, "layer thin film wells" are placed on the Agarose Gel (Fig. 9a) or Polyacrylamide Gel (93) in an overlapped form (Fig. 12). At this time, the "layer thin film well" should have a conductor inside the + electrode direction. This creates a "layer thin film well" in the state shown in Figure 8b, which is cut enough to plug into the Gel, which was done in the fourth experiment.

They are electrophoresed on an arbitrary X-axis line, and-current is left as it is. Connect the power to the conductor of the "thin film well" and supply-current at the time of electrophoresis and + current to the conductor of the thin film well. After enough time for the fragments to gather in the slit, the power was turned off and the buffer or electrolyte in the slit was recovered.

Another final method is to make Agarose or Polyacrylamide Gel Plate as shown in Figure 13a. The "layer thin film well" is composed of the conductor 131 and the non-conductor 132 in the form as shown in Figs. 13B and 13C. Place it on the Gel Plate and either preload it when gelling Agarose or Polyacrylamide, or slice the Gel on the coagulated Gel Plate and place it there. And use insulator as slit. At this time, put "Lamination Film Well" in Gel and harden Gel so that liquid Gel does not enter between Slits. After electrophoresis, confirm the separation of fragments, fill the buffer or electrolyte with slits, and send the flow of charge from the Slits (132) to send the DNA, RNA or Protein Fragments (133) to the Slits (132). Flow towards Fragments (133). Slits 134 then contain DNA, RNA or Protein Fragments 133.

If there are several lanes to be electrophoresed, place several pins between each lane and perform electrophoresis in the same order as above.

The above eight <Experimental Examples> may achieve faster results under the following basic conditions.

 There must be an electrophoretic tank that transmits visible light through UV light. Transparent electrophoretic tanks have already come in many varieties and forms. However, for clearer detection, only the part where the Gel Plate is placed should be transparent and the rest should be an electrophoretic tank that absorbs light. Thus, the present invention is made of only the transparent part of the gel plate, the remaining part is made of a black-based opaque electrophoretic tank.

This is because the gap between the slit of the thin film well is very narrow, and the sharper the shape of the fragments, the more efficient the work can be done without error. In addition, the higher the ISO (ASA) value is, the better the cost will be. Electrophoretic tanks are therefore also useful tools of the present invention.

In the above-mentioned invention, in one step with time saving of the work by integrating the extraction made after the identification step of the next step, RNA, DNA or Protein Fragments in the existing electrophoresis work as a separate and different work into one It was possible to achieve a series of manual tasks that were carried out step by step. In addition, in order to separate and confirm the electrophoretic operation, a task for quantification and purification was added.

In addition, although electrophoretic technology is a part of electrolysis, there was no way to recover the electrolyzed one, but it did not play a role. Epochs have been established.

In particular, by increasing the reproducibility of the work will be a basic technology that can automate each of these manual work, which will be one of the useful inventions in the life sciences.

Claims (21)

Fragments flow together in the direction of the X axis through which current flows on the Agarose Gel. In this case, creating a Well in the direction of the X axis that the Fragments will move, To recover the fragments in the well using a fragment adsorbent, Recovering the buffer solution or the electrolyte solution, How to move on to other tasks using Fragments left in the Well. Fragments flow together in the direction of the X axis through which current flows on the polyacrylamide gel. In this case, creating a Well in the direction of the X axis that the Fragments will move, To recover the fragments in the well using a fragment adsorbent, Recovering the buffer solution or the electrolyte solution, A device that allows you to move to the next step using Fragments left in the Well. On the 2-Dimensions Polyacrylamide Gel, the fragments flow together in the direction of the X-axis, and when the current flows in the Y-axis, the fragments also flow. At this time, the wells are made in the direction of the Y axis Using a fragment adsorbent to recover the fragments in the well, Recovering the buffer solution or the electrolyte solution,  A device that allows you to move to the next step using Fragments left in the Well. By breaking the current from the X axis on the horizontal plane where the current flows on the Agarose Gel and flowing the current in the direction of the Z axis on the vertical line to move the Fragments vertically to the recovery device, To move the Fragments vertically by flowing in the Z-axis for recovery purposes, A device that moves to the Z-axis and moves from the recovery device to the next working stage, not for recovery purposes. By moving the current from the plane X axis on the polyacrylamide gel and flowing the current in the direction of the Z axis on the vertical line to move the fragments vertically to the recovery device, To move the Fragments vertically by flowing in the Z-axis for recovery purposes, A device that moves to the Z-axis and moves from the recovery device to the next working stage, not for recovery purposes. On the 2-Dimensions Polyacrylamide Gel, the current flows from the X-axis on the plane line and another Y-axis on the plane line, and flows the current in the Z-axis direction on the vertical line from the two lines. Moving it vertically,  To move the Fragments vertically by flowing a current in the Z-axis for recovery purposes, A device that moves the Fragments in the Z direction so that it can be moved from the recovery unit to the next working step, rather than for recovery purposes. Moving the Fragments to the recovery device by flowing the current from the direction of the X axis on the horizontal plane in which the current flows on the Agarose Gel in the direction in agreement with the vertical Z axis, Moving the Fragments to the place where the recovery device is located by flowing a current in the direction that is in agreement with the Y axis on the horizontal plane line such as the X axis and the Z axis perpendicular to the horizontal plane, Moving the fragments to the recovery device by flowing electric current in the direction of the Z and the Z axis in either the starting point on the plane with the X and Y axes as the planes, Moving the Fragments for recovery purposes, Arrangements for the transfer from the recovery unit to the next working stage, not for recovery purposes. By moving current from the direction of the X axis on the horizontal plane in which the current flows on the Polyacrylamide Gel to the direction of the summation with the vertical Z axis, Moving the Fragments to the place where the recovery device is located by flowing a current in the direction that is in agreement with the Y axis on the horizontal plane line such as the X axis and the Z axis perpendicular to the horizontal plane, Moving the fragments to the recovery device by flowing electric current in the direction of the Z and the Z axis in either the starting point on the plane with the X and Y axes as the planes, Moving the Fragments for recovery purposes, Arrangements for the transfer from the recovery unit to the next working stage, not for recovery purposes. On the 2-Dimensions Polyacrylamide Gel, the direction of the X axis through which the primary current flows and the direction of the Y axis through which the secondary current flows are viewed as planes, and the vertical direction is set as the Z axis from them. At this time, the electric current flows in the direction of Z axis along with the origin of Z on the plane, and moves the Fragments to the place where the recovery device is located. For the purpose of retrieving fragments, Arrangements for returning to the next working stage, not for recovery purposes. In Agarose Gel electrophoresis, the flow of current flows from the X axis where the current is electrophoresed in the direction of the Y axis on the horizontal plane to move the fragments to the recovery device. Moving the fragments in the Y-axis direction for recovery purposes, A device that can move from the recovery device to the next working stage without moving to the Y axis. In the case of polyacrylamide gel electrophoresis, the flow of current flows from the X-axis where the direction of current is electrophoresis in the direction of the Y-axis on the horizontal plane to move the fragments to the recovery device. Moving the fragments in the Y-axis direction for recovery purposes, A device that can move from the recovery device to the next working stage without moving to the Y axis. Moving the fragments to the recovery device by flowing a current from the 2-Dimensions Polyacrylamide Gel electrophoresis to the recovery device. For the purpose of retrieving fragments, Arrangements for the transfer from the recovery unit to the next working stage, not for recovery purposes. An electrode that can flow current into a container or slit where a buffer or electrolyte is trapped to recover the fragments, The length of the electrode falling into the buffer or electrolyte is 0.01mm or more and 90mm or less The width of the electrode falling into the buffer or electrolyte is 0.01mm or more and 90mm or less. Part of the conductor or slit in which the buffer or electrolyte is trapped to recover the fragments is coated with only a part of the conductor as shown in FIGS. 6A and 6C. A thin film in a container or a container that traps buffers or electrolytes to recover fragments, in which only a part of the conductor is coated as shown in FIG. The thickness of the wall of the container or the slit or the wall where the electrolyte is trapped to recover the fragments from the electrophoresis device is 0.01mm or more and 40mm or less. The distance (84) from the bottom of the well to the bottom of the gel in the recovery device is short, and the length (85) (68) from the bottom bottom of the Y axis in the recovery device to the bottom of the gel is short. In recovery The length of the slit (79) is from 0.4mm to less than 40mm, The length of the slit 79 is not less than 40 times less than 1/5 of the length of the well 80; Slit width is 1/10 or more and 10 times or less of well width. In the Agarose electrophoretic tank, when the direction of current flow for electrophoresis is the X axis, the electrophoresis is performed in which the current flow direction is changed in the direction of the Y axis corresponding to the X axis. Tank. In the electrophoretic tank, a device in which a <-> <+> current flows in the direction of the Z axis in the direction of the X axis or the Y axis. The part where Gel or Gel Plate is placed in electrophoretic tank is made of transparent material to transmit light, and the rest part is made of black or black color to absorb some light.

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