KR20060022369A - Health functional foods to improve skin wrinkles and suppress skin wrinkles - Google Patents

Abstract

The present invention relates to a health functional food exhibiting an effect of improving skin wrinkles and suppressing wrinkle formation upon ingestion, wherein the health functional food for improving skin wrinkles and inhibiting wrinkle formation is pine extract, ascorbic acid, vitamin E and gamma. It is characterized by containing a complex mixed with linoleic acid as an active ingredient. According to the present invention, a health functional food composition containing pine extract, ascorbic acid, vitamin E, and gamma linoleic acid at the same time inhibits collagen degrading enzyme activity when ingested to prevent collagen degradation and also promotes collagen biosynthesis to wrinkle skin. In addition to improving effects and suppressing wrinkle formation, there are no side effects on the human body and are safe.

Dietary supplement, pine extract, ascorbic acid, vitamin E, gamma linoleic acid

Description

Health foods for improving skin wrinkles and suppressing skin wrinkles {Health Foods for the care of skin wrinkle}

The present invention relates to a health functional food composition exhibiting a skin wrinkle improvement effect upon ingestion.

Human skin is changed by a number of internal and external factors as it ages. In other words, intrinsic (endogenous aging or natural aging) secretion of various hormones that regulate metabolism is reduced, the function of immune cells and the activity of cells is reduced, thereby reducing the biosynthesis of immune proteins and biological proteins necessary for the living body, Externally (photoaging) increases the amount of ultraviolet rays in the sun's rays due to the destruction of the ozone layer and increases the free radicals and harmful free radicals as the environmental pollution is intensified. There are many changes, including an increase in blotch.

In general, skin wrinkles are known to be produced by various factors such as skin moisture content, collagen content, ability to act on the external environment, and collagen is one of the biggest influences on skin wrinkle formation.

Collagen is a fibrous tissue that gives skin flexibility and is a major component of about 70% of dermal tissue. Polypeptide chains of collagen fibers are synthesized in the fibroblast granular reticulum with the extended peptides (telopeptide) at both ends, and then secreted out of the cells in the form of procollagen combined into a triple helix structure. The secreted procollagen is digested with an extended peptide by a specific enzyme to produce tropocollagen, which is combined with each other in the extracellular matrix and synthesized into collagen fibers.

Synthesis of the collagen fibers is rapidly reduced as the skin ages, and as the collagen fibers are deformed by the external environment, a lot of wrinkles are generated and deepened in the skin. The collagen is involved in collagenase, which is a collagenase.

Recently, many skin physiological studies have been conducted on the cause and improvement methods of skin wrinkles. As a solution for this, skin wrinkle improvement methods have been proposed by external methods such as cosmetics, but the substances exhibiting skin wrinkle improvement effects by dietary intake. Very little research has been conducted on.

For example, retinol, which is widely used to improve skin wrinkles and pigmentation, is commercialized as a cosmetic composition and is used to improve skin wrinkles.A cosmetic composition containing ascorbic acid, which is essential for collagen synthesis, is used for wrinkle improvement, sun protection, and skin pigmentation. There are reports of effectiveness (US Pat. No. 4,938,969, US Pat. No. 4,772,591, European Patent No. 0533667, etc.).

However, the active ingredients of the skin wrinkle improvement have been used in most external skin prescriptions, such as cosmetic compositions, and when used as a cosmetic composition, there are many problems in the safety and stability, and the effect is also insufficient. .

Therefore, the technical problem to be achieved by the present invention is to solve the above problems, to inhibit the activity of collagen degrading enzyme (collagenase) to prevent the degradation of collagen and also to improve the skin wrinkles by promoting collagen biosynthesis, To provide a health functional food composition for improving skin wrinkles safe to the human body when ingested.

In order to achieve the above object, the skin functional food composition for improving wrinkles of the present invention contains a complex of pine extract, ascorbic acid, vitamin E and gamma linoleic acid as an active ingredient.

Wrinkle improvement activity has a unique mechanism of action, the effect of the various components of the action mechanism can be clearly seen the difference in effect. Therefore, the effect may be enhanced or antagonistic due to the interaction through the combination of appropriate wrinkle improvement ingredients. Pine extract, ascorbic acid, vitamin E and gamma-linoleic acid of the present invention, when ingested four ingredients as a safe substance to the human body at the same time, has a synergistic effect on skin wrinkle improvement by the interaction through a combination, has a strong wrinkle improvement effect Indicates.

Pine extract inhibits the activity of collagenase, prevents the breakdown of collagen, a skin protein, and reduces the elasticity of the skin. Also, it has strong antioxidant power and has the function of regenerating ascorbic acid and vitamin E. . Ascorbic acid and vitamin E have strong anti-oxidant properties and inhibit free wrinkles due to skin aging by removing free radicals and harmful free radicals that cause photoaging. Gamma-linoleic acid metabolizes the skin and converts it into 13-HODEE (13-Hydroxyoctadecadienoic acid) to form ceramides, etc. Give it.

The complex contained in the functional composition for improving skin wrinkles of the present invention is 1 to 45 parts by weight of pine extract, 18 to 75 parts by weight of ascorbic acid, 4 to 35 parts by weight of vitamin E and gamma-linoleic acid based on 100 parts by weight of the complex It is preferable to use those mixed in the proportion of 2 to 45 parts by weight.

Hereinafter, the health functional food composition for improving skin wrinkles according to the present invention will be described in detail.

In the health functional food composition for improving skin wrinkles of the present invention, the pine extract, ascorbic acid, vitamin E and gamma linoleic acid which act as an active ingredient will be briefly described as follows.

Pine extract is extracted from the pine belonging to the genus Pinus, and various papers have been published regarding Pycnogenol, a French pine algae extract. Pycnogenol's effects include circulatory vascular-related improvement (Fitzpatrick et al., 1998), antioxidant effects (Chida et al., 1999; Cossins et al., 1998; Devarj et al., 2002), skin blackening and inflammation inhibition (Saliou et al., 2001; Ni). Et al., 2002) and collagen proteolytic inhibitory effects (Grimm et al., 2002). Pine extract is the main raw material on the food raw materials, the main components are tannins and flavonoids, including proanthocyanidins, and are harmless to humans have been used from whales. Pine extract can be obtained by, for example, cooling or warming in a solvent such as water and / or ethanol to extract the active ingredient, filtering the filtrate, and then concentrating the filtrate under reduced pressure.

In addition, ascorbic acid and vitamin E are vitamins with excellent antioxidant effects and are not harmful to the human body. Ascorbic acid and vitamin E are also known as whitening materials, and there is also a Korean Patent Publication (KR 2004-0040557) which provides anti-aging cosmetic composition and wrinkle improvement using ascorbic acid and its derivatives.

Gamma-linoleic acid is defined as an unsaturated fatty acid with functional properties that promote cholesterol improvement and blood circulation in the current dietary supplement, and is contained in large amounts in evening primrose oil, borage oil, pumpkin seed oil, and burdock oil. Gamma-linoleic acid is converted to 13-HODE (13-Hydroxyoctadecadienoic acid) in the epidermis of the skin and then incorporated in phospholipids to inhibit skin hyperproliferation and also have a skin moisturizing function It is.

The present inventors mainly consumed ascolic acid, which has been used as an external preparation applied directly to the skin, such as cosmetics, and pine extract, vitamin E and gamma linoleic acid, which are known to be excellent in antioxidant effects, as a result of dietary intake, as well as excellent wrinkle improvement effect. The present invention has been completed by discovering that each other exhibits a strong synergistic effect.

In other words, the wrinkle improving activity of the wrinkle improving component has a unique mechanism of action, the effect of these wrinkle improving components due to mutual mixing or may exhibit an antagonistic effect. Pine extract, ascorbic acid, vitamin E and gamma-linoleic acid are safe substances for humans, and when they are ingested at the same time, they have a strong synergistic effect on skin wrinkle improvement.

In the composition for dietary intake of the present invention, the four skin wrinkle improvement ingredients described above are 1 to 45 parts by weight of pine extract, 18 to 75 parts by weight of ascorbic acid, 4 to 35 parts by weight of vitamin E and 2 to 45 parts by weight of gamma linoleic acid. It is preferable to mix in negative ratio. If the amount below the recommended minimum amount of each ingredient is used, it is difficult to expect wrinkle improvement by synergistic effect.If the amount above the recommended maximum amount of each ingredient is used, wrinkle improvement effect is directly proportional to the increase in the content used. It is not increasing and is not commercially suitable because the price of the raw material is too expensive.

Health functional food composition for improving skin wrinkles of the present invention can be prepared in the form of tablets, granules, powders, capsules, liquid solutions such as beverages, pills, etc. according to a known manufacturing method, four components according to the present invention The content of the complex consisting of may be adjusted to 0.2 to 100% by weight based on the total weight of the health functional food composition depending on the formulation. Here pine extract is based on dry weight.

As an example of preparation of the composition, a mixture of pine extract, ascorbic acid, vitamin E and gamma linoleic acid is mixed with soybean oil, beeswax, lecithin and the like, which are commonly used as excipients, and then the mixture is injected into a gelatinous coating. By this, a normal soft capsule can be produced. In addition, in order to prepare the composition of the above various formulations, each component may be used in place of a derivative having the same efficacy. For example, ascorbic acid may be used in place of sodium L-ascorbate, L-ascorbate stearate, potassium ascorbate, calcium ascorbate, palmitate ascorbate, and the like.

Hereinafter, the present invention will be described in detail with reference to experimental examples and examples. However, embodiments according to the present invention can be modified in many different forms, the scope of the present invention should not be construed as limited to the embodiments described below. Embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art.

Experimental Example 1 Comparison of Antioxidant Activity of Pine Extracts

In order to compare the antioxidant power of various pine extracts, Korean pine extract, Chinese pine extract and French seaweed extract (trade name Pycnogenol) were purchased among various pine extracts.

Antioxidant activity was measured by the method using DPPH (1,1-Diphenyl-2-picryl-hydrazyl) radical scavenging effect.

 4 mg of DPPH was dissolved in 100 ml of methanol to make a DPPH solution. The extract sample was diluted in ethanol to an appropriate concentration. After mixing 1 ml of the sample or the control and 1 ml of the DPPH solution, the mixture was allowed to stand at 37 ° C. for 30 minutes and the absorbance was measured at 516 nm. BHT (Butylated Hydroxytoluene) was used as a standard, and ethanol was used as a control.

The antioxidant power by DPPH method was calculated as follows.

Antioxidant power (%) = 100-(absorbance of sample / absorbance of control) X 100

The results of the antioxidant test determined by measuring the absorbance as described above are shown in Table 1 below.

Sample Name density(%) Antioxidant power (%) Domestic pine extract 0.01 95.1 0.001 56.0 0.0001 5.6 Chinese Pine Extract 0.01 89.9 0.001 45.0 0.0001 5.1 Pycnogenol 0.01 94.8 0.001 61.0 0.0001 8.9 BHT 0.01 82.9 0.001 27.6 0.0001 1.1

As shown in Table 1, as a result of comparing the antioxidant power through the radical scavenging power by the DPPH method, all pine extracts showed a significant antioxidant power. In particular, since the antioxidant power of Pycnogenol, a French seaweed extract, was excellent, Pycnogenol was used as a pine extract in subsequent tests.

[Test Example]

Pine extract (Pinogenol), ascorbic acid, vitamin E and gamma linoleic acid were mixed in a total amount of 100 mg according to the content ratio (unit: weight percent) described in Table 2 below, and then the collagenase enzyme collagenase activity Inhibitory effect was tested. In addition, the efficacy was compared with doxycycline, which is used as an inhibitor of collagenase activity in medicines. Pycnogenol was used as a pine extract, and as a gamma linoleic acid, evening primrose oil (containing 59% gamma linoleic acid) was used.

Pine Extract (Pycnogenol) Ascorbic Acid Vitamin E Gamma-linoleic acid Test Example 1 100 0 0 0 Test Example 2 0 100 0 0 Test Example 3 50 50 0 0 Test Example 4 50 0 50 0 Test Example 5 0 50 0 50 Test Example 6 33.3 33.3 33.4 0 Test Example 7 33.3 0 33.3 33.4 Test Example 8 0 33.3 33.3 33.4 Test Example 9 25 25 25 25 Test Example 10 10 40 10 40 Test Example 11 40 20 30 10 Test Example 12 5 50 15 30

Experimental Example 2 Inhibitory Effect on Fibroblast Collagenase (MMP-1)

The inhibitory effect on fibroblast collagenase (MMP-1) in mammals was measured by the following method.

  100 μl of Azocoll solution, 2% red collagen substrate, was added to 17 1.5 ml Eppendorf tubes, and one Eppendorf tube was used as a blank, and the standard activity curve of the enzyme was used. Three tubes were added to 10, 100 and 200 ppm of collagenase enzyme standard solutions purchased from Calbiocam, respectively. Each of the remaining 13 tubes was treated with 10 μl of collagenase enzyme standard solution, Test Examples 1 to 12 and doxycycline at a concentration of 0.005%. Buffer solution (0.05M Tris-HCl, 1nM CaCl2, pH 7.8) was added to make 500µl of total reaction solution and reacted for 18 hours at 37 ℃ thermostat, and then the Eppendorf tube was centrifuged at 10,000g for 5 minutes. Collagen was precipitated. A supernatant solution containing digested collagen was taken to measure absorbance at 540 nm to prepare a standard activity curve, and the enzyme activities of Test Examples 1 to 12 and doxycycline and control were compared by evaluating the activity concentration of enzyme from the standard curve.

Collagenase enzyme inhibition rate was calculated as follows.

Enzyme inhibition rate (%) = 100-(Experiment enzyme activity / control enzyme activity X 100)

The results are shown in Table 3.

sample Collagenase enzyme inhibition rate (%) Doxycycline 47 Test Example 1 28 Test Example 2 27 Test Example 3 45 Test Example 4 45 Test Example 5 46 Test Example 6 68 Test Example 7 70 Test Example 8 68 Test Example 9 90 Test Example 10 85 Test Example 11 86 Test Example 12 84

Through the results in Table 3, pine extracts (Pinogenol), ascolic acid, vitamin E and gamma-linoleic acid for the collagenase enzyme when all four types were used than when used alone or in combination of two or three It turned out that it shows a strong suppression rate. In other words, when all the components were used, the synergistic effect between the two showed an excellent rate of enzyme activity inhibition. In addition, it showed much higher enzyme inhibition rate than doxycycline.

Experimental Example 3 Collagen Biosynthesis Effect

Pine extract (Pinogenol), ascorbic acid, vitamin E and gamma linoleic acid were mixed in a total amount of 100 mg according to the content ratio (unit: weight%) described in Table 2 above, and then in the culture medium of human-derived fibroblasts Collagen biosynthesis effects were tested.

Human-derived fibroblasts were dispensed into 24-well plates for cell culture, incubated for 24 hours under 10% FBS, and washed twice with PBS. The cultures were terminated after 24 hours of incubation by adding 0.001% of Test Groups 1 to 13 to a medium containing 1% FBS. After the incubation, the cells and the culture medium were divided into half and treated with collagenase in only one fraction, followed by precipitating the protein with trichloroacetic acid (TCA) to measure collagen biosynthesis promoting effect from the difference in radioactivity of the two fractions. , The control group was not added to the test group to 100% the results are shown in Table 4.

Collagen production rate (%) = Collagen production amount of test group / Collagen production amount of control group X 100

sample Collagen production rate (%) Control 100 Test Example 1 106 Test Example 2 107 Test Example 3 115 Test Example 4 115 Test Example 5 115 Test Example 6 134 Test Example 7 133 Test Example 8 133 Test Example 9 176 Test Example 10 173 Test Example 11 174 Test Example 12 174

As can be seen from the results in Table 4, the collagen biosynthesis rate increased rapidly when all four species were used compared to the pine extract (Pinogenol), ascorbic acid, vitamin E and gamma-linoleic acid alone or in combination of two to three species. It can be seen that this is due to the synergistic effect of each component.

Experimental Example 4 Wrinkle Formation Inhibitory Effect

In order to confirm the anti-wrinkle effect of the mixed samples of pine extract (Pinogenol), ascorbic acid, vitamin E and gamma linoleic acid, hairless mice were tested for anti-wrinkle formation.

Ten hairless mice of 6 weeks old were divided into test groups and control groups, and hairless mice were irradiated with 2MED for 3 days and 10 days a week using a solar stimulator to form wrinkles. The diet was freely fed the normal hairless mouse feed, and the control sample and the test group sample were forcibly administered for 10 weeks from the day of ultraviolet irradiation using the zonal band, 30 minutes before the ultraviolet irradiation on the day of ultraviolet irradiation. Determination of the degree of wrinkle formation inhibition was visually determined by visual inspection and photographing of the sample treatment area, and the criterion was no inhibition (0 point), slightly compared to the control group (0 point). The number of animals was determined in four stages: suppression (1 point), moderate suppression (2 points), and significant suppression (3 points). The test sample was 60 mg / kg and 600 mg / kg of pine extract (Pinogenol), ascorbic acid, vitamin E and gamma linoleic acid, respectively, based on the results of Experiments 1 and 2 and the daily intake of raw materials allowed by the dietary supplement. , 70 mg / kg and 240 mg / kg. Water was used as a control.

Experimental group Wrinkle suppression (mari) 0 points 1 point 2 points 3 points Test group 0 One One 8 Control 10 0 0 0

As can be seen from the results in Table 5, the inhibition of wrinkle formation in hairless mice by the test group ingested pine extract (picinogenol), ascorbic acid, vitamin E and gamma linoleic acid showed an effect of inhibiting moderate to moderate wrinkle formation at 80% or more. It was confirmed that it has an excellent effect on suppressing wrinkle formation.

Experimental Example 5 Wrinkle Improvement Effect

In order to confirm the wrinkle improvement effect of the mixed samples of pine extract (Pinogenol), ascorbic acid, vitamin E and gamma linoleic acid, hairless mice were tested for wrinkle improvement effect.

Ten hairless mice of 6 weeks old were divided into test groups and control groups, and hairless mice were irradiated with 2MED for 3 days and 10 days a week using a solar stimulator to form wrinkles. The diet was freely fed normal hairless mouse feed, and control and test samples were forced to use for 6 weeks using the zonal. Judgment of the degree of wrinkle improvement was visually determined by visual inspection and photographing of the sample treatment area, and the criterion was no improvement (0 point), slight improvement (1 point), compared to the test group treatment group and the control group before the sample treatment. The number of animals was determined in four stages of moderate improvement (2 points) and significant improvement (3 points), and is shown in Table 6. The test sample was 60 mg / kg and 600 mg / kg of pine extract (Pinogenol), ascorbic acid, vitamin E and gamma linoleic acid, respectively, based on the results of Experiments 1 and 2 and the daily intake of raw materials allowed by the dietary supplement. , 70 mg / kg and 240 mg / kg. Water was used as a control.

Experimental group Wrinkle Improvement (Marie) 0 points 1 point 2 points 3 points Test group 0 One 2 7 Control 9 One 0 0

As can be seen from the results of Table 6, the test group ingested pine extract (Pinogenol), ascorbic acid, vitamin E and gamma-linoleic acid had an effect of improving moderate to moderate wrinkles at 80% or more for the wrinkles generated by the light of hairless mice. It was confirmed that.

[Production Example 1]

The above-mentioned pine extract, ascorbic acid, vitamin E and gamma-linoleic acid as a main component to prepare a soft capsule in the content shown in Table 7 below. The pine extract used Pycnogenol, a French pine algae extract, used vitamin E oil containing 70% by weight of D-α-tocopherol, and gamma-linoleic acid was used as evening primrose oil containing 59% by weight of gamma-linoleic acid. .

Soybean oil, beeswax and lecithin were used as excipients.

Raw material Content (mg) Content ratio (%) Mixed contents (total amount: 360mg) Pine Extract (Pycnogenol) 15.001 4.167 Ascorbic Acid 150.001 41.667 Vitamin E (70% by weight) 25.002 6.945 Evening primrose oil (containing 59% gamma-linoleic acid) 100.001 27.778 Soybean oil 51.995 14.443 Beeswax 14.400 4.000 lecithin 3.600 1.000 Soft capsule base (total amount: 185mg) gelatin 185 68.850 glycerin 21.862 Manatol 7070 8.197 Ethylvanillin 0.164 Titanium dioxide 0.344 Food coloring Blue No. 1 0.004 Food coloring Blue No. 3 0.028 Food coloring Red 40 0.551

As shown in Table 7, the mixed contents were mixed and mixed in a stirrer, and then injected into the soft capsule base to prepare a soft capsule.

The preparation example is not a limitation of the formulation of the present invention as a conventional preparation for producing a health functional food for improving the skin wrinkles and inhibiting wrinkle formation.

As described above, the health functional food composition of pine extract, ascorbic acid, vitamin E and gamma linoleic acid according to the present invention has a strong wrinkle improvement synergistic effect, functional food showing the skin wrinkle improvement and wrinkle production inhibitory effect It can be usefully used.

Claims (5)

A health functional food for improving skin wrinkles and inhibiting skin wrinkles, comprising a complex of pine extract, ascorbic acid, vitamin E and gamma-linoleic acid as an active ingredient. The skin of claim 1, wherein the complex is mixed in a ratio of 1 to 45 parts by weight of pine extract, 18 to 75 parts by weight of ascorbic acid, 4 to 35 parts by weight of vitamin E, and 2 to 45 parts by weight of gamma linoleic acid. Health functional food to improve wrinkles and suppress skin wrinkles. According to claim 1 or 2, wherein the content of the complex is a functional food for skin wrinkle improvement and skin wrinkle production, characterized in that 0.2 to 100% by weight based on the total weight of the health functional food. According to claim 1, wherein the pine extract is a health functional food for improving skin wrinkles and inhibiting the production of skin wrinkles, characterized in that the extract from France. According to claim 1, wherein the health functional food for improving skin wrinkles and inhibiting skin wrinkles, characterized in that it is made of any one formulation selected from the group consisting of tablets, granules, powders, capsules, liquid solutions and pills. Health functional food.