KR20060007083A - Whitening cosmetic composition containing rambutan extract and rambutan extraction method - Google Patents

Abstract

The present invention relates to a whitening cosmetic composition and a method of extracting rambutan containing rambutan extract ( Nephelium lappaceum L, var. Lappaceum ).

The whitening cosmetic composition containing the rambutan extract of the present invention not only inhibits tyrosinase involved in the skin blackening process, but also inhibits melanin production, and is excellent in whitening efficacy and has no irritation to the skin. The safety is also excellent.

Rambutan, Tyrosinase Inhibition, Melanin Inhibition, Skin Whitening, Skin Blackening

Description

Whitening cosmetic composition containing rambutan extract and method for extracting rambutan {SKIN-WHITENING COSMETIC COMPOSITION CONTAINING EXTRACT OF RAMBUTAN AND METHOD FOR EXTRACTING RAMBUTAN}

The invention rambutan (Nephelium lappaceum L, var. Lappaceum, Rambatan) relates to a whitening cosmetic composition and rambutan extraction containing extract, more particularly, to suppress melanin production and at the same time suppressing the tyrosinase it is excellent in whitening effect rambutan ( Nephelium lappaceum L.) relates to a whitening cosmetic composition containing the extract and rambutan extraction method.

Skin is the body's primary protective film that protects internal organs from changes in temperature and humidity, and from stimuli from the external environment such as ultraviolet rays and pollutants, and plays an important role in maintaining homeostasis such as body temperature control. However, cell damage, such as excessive physical stimuli, chemical stimuli, stress, malnutrition, ultraviolet radiation, environmental pollutants, and sudden changes in temperature caused from outside the cell, induce a variety of biochemical changes in the cell, causing a cell damage response. One such skin problem is skin blackening such as spots, freckles, and blemishes.

Such blackening of the skin, such as blemishes and freckles, is due to melanin. Melanin in the epidermal layer, which is the outer skin of the skin, acts as a sunscreen to protect the skin organs below the dermis and also to protect free radicals generated in the skin, thereby protecting proteins and genes in the skin. . When the skin is exposed to ultraviolet rays, an enzyme called tyrosinase acts on tyrosine, a kind of amino acid. In the melanosomes (melanocyte), a polymer called melanin is synthesized. In addition, the production of free radicals in the skin increases, melanin production increases when there is an inflammatory reaction or ultraviolet rays.

As the causes and mechanisms of skin blackening have been found, substances having an effect of inhibiting tyrosinase, an enzyme involved in skin blackening, or inhibiting melanin polymerization, have been used in cosmetics. Representative materials used for this purpose include a hydroquinone, ascorbic acid, kojic acid, or a thiol-based compound such as glutathione, cysteine, and alpha hydroxy acid. However, most of these ingredients are known to be unstable by themselves or have a weakening whitening effect and cause skin irritation. In addition, natural extracts known to have a whitening effect contain a small amount of active ingredients, and the content of the extract is severe depending on the place of production or the harvest time, making it difficult to have homogeneity of quality. Has the disadvantages.

In order to solve the problems of existing melanin production inhibitors such as weak whitening effect, low stability, skin irritation, the object of the present invention is a whitening cosmetic containing rambutan extract showing excellent whitening effect without skin irritation It is to provide a composition.

Another object of the present invention is to provide a rambutan extraction method that can increase the content of the active ingredient of rambutan extract and increase skin safety.

In order to achieve the above object, the present invention provides a whitening cosmetic composition containing rambutan extract ( Nephelium lappaceum L, var. Lappaceum ) as an active ingredient.

The present invention also provides (a) adding at least one selected from an extract solvent consisting of anhydrous, hydrous lower alcohol, a mixture of water and lower alcohol, acetone and 1,3-butylene glycol having 1 to 4 carbon atoms to the rambutan dried product. Then extracting by heating for 3 to 24 hours at 50 ~ 200 ℃ in a distillation apparatus attached to the cooling capacitor; (b) aging the extract of step (a) for 1 to 10 days at 4 ~ 25 ℃; (c) the aging solution of step (b) is concentrated under reduced pressure at 50-90 ° C. in a distillation apparatus equipped with a cooling capacitor, and at least one time is selected from an extraction solvent consisting of ethyl acetate, diethyl ether and methylene chloride. Extracting; And (d) concentrating and drying the organic layer in the extract of step (c) under reduced pressure. It provides a method for producing a rambutan extract comprising a.

Hereinafter, the present invention will be described in detail.

The present inventors have while trying to overcome the problems of weak bleaching effect, low stability, skin irritation with their conventional bleaching substances without causing the skin side effects research on natural products having excellent skin whitening effect, rambutan (Nephelium lappaceum L ., Rambatan) extract was found to have a whitening effect and developed an extract preparation method for maximizing its safety and efficacy, to complete the present invention based on this.

The whitening cosmetic composition according to the present invention contains a rambutan extract as an active ingredient.

Rambutan (Nephelium lappaceum L, var. Lappaceum , Rambatan) is rambutan (Nephelium lappaceum L.) is. Examples of the plant belonging to sapindaceae (Sapindaceae), plants classified haksang glabrum Cambess N., N. chryseum Blume, N. sufferrugineum Radlk. Has a different name (synonym). Rambutan is 10 ~ 15m tall, leaves are alternate, 5 ~ 7 pairs of idol, and thick, glossy, with hair on the back when young. Flowers bloom in April-June and are biphasic (miscellaneous flowers) or hybrid flowers, and are yellowish green on the inflorescences (圓錐 花序). Fruits are gathered by 10-12 pieces and run as small eggs. They ripen in red in July-August and have meaty processes. Rambutan is one of the most well-known fruits of tropical fruits in Southeast Asia. Cultivated fruits are usually used for reproduction and can be used for cooking syrup as well as canned syrup. The skin of rambutan contains tannin and saponin, which is used as a herbal medicine. In addition, the root is used as a medicine to treat colds. The leaf is used as a poultice, and the bark is used to relieve muscles when the tongue develops. use. However, there is no example of using the extract of rambutan in whitening cosmetics for the purpose of removing blemishes, freckles and various pigmentation.

Rambutan (Nephelium lappaceum L.) extract according to the invention shows an excellent skin whitening effect by suppressing melanin production as well as inhibits the tyrosinase involved in skin blackening process without skin irritation. The cosmetic composition of the present invention containing rambutan extract can be used for improving and preventing skin pigmentation such as blemishes, freckles, blemishes and skin burns.

The whitening cosmetic composition of the present invention is characterized by containing rambutan extract in an amount of 0.001 to 10% by weight, preferably 0.01 to 5% by weight based on the total weight of the cosmetic composition. When the content of rambutan extract is less than 0.001% by weight, the skin whitening effect is insignificant, and when it exceeds 10% by weight, the synergistic effect due to the excessive injection is not economical. Meanwhile, the rambutan extract may use a rambutan fruit extract including the pulp and the rind, and particularly, the rambutan rind extract is preferable in terms of skin whitening effect.

Extraction method for obtaining a rambutan extract of the present invention is as follows.

(a) First extraction step

The rambutane washed with clean water is crushed and dried to obtain a dry product. In the rambutan dry matter, one or more anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms, for example, normal propanol, isopropanol, a mixture of water and a lower alcohol, acetone and 1,3-butylene glycol are selected, and at least one is selected. 20 times by volume is added, and then extracted by heating to 50 ~ 200 ℃ for 3 to 24 hours in a distillation apparatus with a cooling capacitor.

(b) ripening step

After filtering the extract of step (a) with a filter cloth, the filtrate is left to mature for 1 to 10 days at 4 ~ 25 ℃ to extract the active ingredient.

(c) secondary extraction step

After filtering the aging liquid of step (b) and concentrated under reduced pressure at 50 ~ 90 ℃ in a distillation apparatus with a cooling capacitor. The concentrated solution is dispersed in water, and at least one extractant consisting of ethyl acetate, diethyl ether and methylene chloride is selected and placed in a separatory funnel and extracted one or more times. The water layer is placed in a separatory funnel and at least one extraction solvent is selected from an extraction solvent consisting of ethyl acetate, diethyl ether and methylene chloride.

(d) drying step

The organic layers in the extract of step (c) were combined, dried over anhydrous sodium sulfate as a drying agent, and filtered and concentrated under reduced pressure. The concentrate is lyophilized or spray dried to obtain a rambutan extract.

Rambutane was first extracted with anhydrous or hydrous lower alcohol and acetone as primary extraction solvent, and then ethyl acetate, dietel ether and methylene chloride, which are solvents of lower polarity than the primary extraction solvent, were used as secondary extraction solvent. By extracting the tea, it is possible to increase the content of the active ingredient, remove impurities, improve stability, and maximize the whitening effect of the rambutan extract.

The whitening cosmetic composition of the present invention contains a cosmetically and dermatologically acceptable medium and / or base. These are all formulations suitable for topical application, for example emulsions, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes) obtained by dispersing an oil phase in a solution, gel or solid or pasty anhydrous product, aqueous phase and / or Or in the form of a nonionic vesicle dispersant, or in the form of a cream, skin, lotion, powder, ointment, essence, spray or cone stick. The composition according to the invention can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

Compositions of the present invention include fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers. Cosmetics such as fillers, metal-ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or any other ingredients commonly used in cosmetics or It may contain adjuvants commonly used in the field of dermatology. These adjuvants are introduced in amounts commonly used in the cosmetic or dermatological field.

Hereinafter, preferred embodiments of the present invention will be described. However, the following examples are only one preferred embodiment of the present invention and the present invention is not limited to the following examples.

(Example)

(Example 1)

Wash the rambutan ( Nephelium lappaceum L.) with purified water, crush it, dry it, and then put 50 g of dried rambutan ( Nephelium lappaceum L.) in 1 L of 50% ethanol aqueous solution and boil it at 100 ° C for 12 hours in an extractor equipped with a cooling condenser. After extraction, the mixture was filtered through a 300 mesh filter cloth. The filtrate was left at 10 ° C. for 7 days to mature and filtered. The filtrate was concentrated under reduced pressure at 60 ° C. using a distillation apparatus equipped with a cooling condenser, and the concentrate was dispersed in 50 ml of water, and then extracted with 100 liters of ethyl acetate. The water layer was put back into the separating funnel and extracted twice more with ethyl acetate. The separated organic layers (ethyl acetate layer) were combined, dried over anhydrous sodium sulfate, filtered, the filtrate was concentrated under reduced pressure, and the concentrated solution was lyophilized to obtain a dry weight of 5.4 g.

Example 2

After washing rambutan ( Nephelium lappaceum L.) with purified water, crushing and drying, 50 g of dried rambutan ( Nephelium lappaceum L.) dried in a 50% ethanol aqueous solution 2L and boiled at 100 ℃ in an extractor with a cooling condenser for 12 hours After extraction it was filtered. The filtrate was left at 10 ° C. for 7 days to mature and filtered. The filtrate was concentrated under reduced pressure using a distillation apparatus equipped with a cooling condenser at 80 ° C., the concentrate was dispersed in 200 ml of water, and placed in a 3 L separatory funnel, 1.5 L of methylene chloride was added, shaken well, and then completely separated into two layers. After the methylene chloride layer was taken. The water layer was put back into the separating funnel and extracted twice more with methylene chloride. The combined organic layers (methylene chloride layer) were combined, dried over anhydrous sodium sulfate, filtered and the filtrate was concentrated under reduced pressure and dried to obtain 4.7 g of dry weight.

(Example 3)

Wash the skin of rambutan ( Nephelium lappaceum L.) with purified water, crush it, dry it, and then put 50 g of dried rambutan ( Nephelium lappaceum L.) in 2 l of 50% ethanol water solution to 100 ° C in the extractor with cooling condenser. Boil for 12 hours, extract and filter. The filtrate is left at 10 ° C. for 7 days to mature and then filtered. The filtrate was concentrated under reduced pressure using a distillation apparatus equipped with a cooling condenser at 80 ° C., the concentrate was dispersed in 200 ml of water, placed in a 3 L separatory funnel, 1.5 L of diethyl ether was added, shaken well, and then completely separated into two layers. After taking the diethyl ether layer. The water layer was put back into the separating funnel and extracted twice more with diethyl ether. The combined organic layers (diethyl ether layer) were combined, dried over anhydrous sodium sulfate, dried and filtered. The filtrate was concentrated under reduced pressure and dried to obtain 4.5 g of dry weight.

(Example 4)

Washed and dried rambutan (Nephralium lappaceum L.) with purified water, and dried 50 g of dried rambutan ( Nephelium lappaceum L.) in 1 l of 80% ethanol, extracted by boiling at 90 ° C for 12 hours in an extractor equipped with a cooling condenser. It was. The filtrate was left at 10 ° C. for 7 days to mature and filtered. The filtrate was concentrated under reduced pressure and dried to obtain a dry weight of 5.8 g.

(Example 5)

Rambutane ( Nephelium lappaceum L.) was washed with purified water, dried, and then 100 g of dried rambutan ( Nephelium lappaceum L.) dried in 5 L of 80% methanol, which was deposited at room temperature for 24 hours, and filtered. The residue was extracted once more in the same way. Each extract was combined and the insoluble matters were removed, concentrated under reduced pressure and dried to give a dry weight of 11.2 g.

       Hereinafter, the following experiment was performed to determine the skin safety and skin whitening effect of the rambutan extract according to the present invention.

Experimental Example

Experimental Example 1

(Safety of the Extract of Ramphetan ( Nephelium lappaceum L.)

Since raw materials for cosmetics are used in the human body, safety for the human body is important above all. Therefore, the following experiment was performed to determine the toxicity and irritation of the rambutan dried extract to the human body. The rambutan extract of Example 1 was performed with a solution made at 10% concentration in propylene glycol / ethanol (8/2).

1) Acute oral toxicity test in mice: When 1 ml / Kg of test substance was administered to 10 rats of 5 males and 5 females, no dead animals were observed and there was a significant difference in body weight before and after administration. Not observed.

2) Primary skin irritation test: Twelve rabbits were removed 24 hours before application of the test substance and observed by applying 0.1 ml each for 2.5 hours at 2.5 cm × 2.5 cm width. Observation determined that there was no stimulus.

3) Eye irritation test (Eye irritation test): 9 rabbits were diluted in saline at a concentration of 2% by weight and administered to the eye 0.1 ml per animal. Experimental results showed no specific eye mucosal irritation to cornea, iris and conjunctiva.

Skin sensitization test: Skin sensitization test was performed in three of each of the three male and female gunea pigs according to Magnusson's and Kligman's test methods.

Human patch test: Thirty healthy women aged 20 to 28 years were tested according to CTFA Guideline (The Cosmetic Toiletry and Fragrance Association, INC, Washington, D. C. 20036, 1991). As a result, skin primary irritation did not occur.

6) Repeat Insult Human Patch Test: The cumulative stimulus response and sensitization response were not observed in human patch test subjects according to CTFA Guidelines.

In the toxicity and skin safety experiments, it was confirmed that rambutan extract was a safe substance for external application due to almost no skin irritation.

Experimental Example 2

(Inhibitory effect on skin pigment cell tyrosinase activity)

Of DMEM with a 10% FBS containing the culture medium by two ^{6-well plate 2 × 10 5} cell / well, 96-well plate to put the 2 × 10 ⁵ cell / well in B16 / F10 melanoma cell 37 ^o C to It was maintained for 24 hours. Here, Examples 1 to 4 and arbutin were treated and cultured for 5 days. When the incubation was completed, washed twice with Phosphate buffered saline (PBS), harvested cells and centrifuged to form a cell precipitate. The precipitate was treated with lysis buffer (50 mM sodium phosphate buffer (pH 6.8) containing 0.1 mM PMSF and 1% Triton X-100) to destroy cells, and centrifuged to obtain supernatant. 100mM sodium phosphate buffer (pH 6.5), 1.5mM L-tyrosine solution and the supernatant were mixed with the supernatant, and the absorbance was measured at 490nm after maintaining at 37 ° C for 30 minutes. After culturing B16 / F10 melanoma cells that were not treated with the sample as a control, the inhibition rate of tyrosinase activity for each sample concentration was determined according to the following equation, and the results are shown in Table 1 below.

TABLE 1 Tyrosinase Inhibitory Activity

Inhibitory Activity (%) Remarks 50 ppm 100 ppm Example 1 43.20 71.39 Secondary extract Example 2 45.23 72.88 Secondary extract Example 3 44.35 77.20 2nd extract (peel) Example 4 35.14 39.94 Primary extract Arbutin 10 23 Whitening cosmetic raw materials

As shown in Table 1, Examples 1 to 4 of the rambutan extract showed an inhibitory effect on tyrosinase activity and showed superior inhibitory activity compared to arbutin used as a raw material of a whitening cosmetic. In addition, Examples 1 to 3 extracted by the extraction method according to the present invention showed a superior tyrosinase inhibitory activity compared to Example 4 which is the primary extract, and Example 1 to Rambutan fruit extract, Examples 1 to 3, which were rambutan fruit extracts. Better tyrosinase inhibition accuracy than Example 2.

Experimental Example 3

(Melanin production inhibitory effect)

Using DMEM containing 10% FBS as a culture medium, B16 / F10 melanoma cells were added at 2 × 10 ⁵ cell / well in two 6-well plates and 2 × 10 ⁵ cell / well in 96-well plate and maintained at 37 ° C. Incubated for 24 hours. Here, Examples 1 to 4 and arbutin were treated and cultured for 5 days. When the incubation was completed, washed twice with Phosphate buffered saline (PBS), harvested cells and centrifuged to form a cell precipitate. This precipitate was treated with 1N NaOH to lyse intracellular melanin. The solution was measured for absorbance at 405 nm, and cultured B16 / F10 melanoma cells, which were not treated with the sample, were used as a control to determine the amount of melanin produced in the sample, and the results are shown in Table 2.

[Table 2] Melanin production inhibitory effect

sample % Inhibition Remarks 10 ppm 50 ppm Example 1 21.69 47.25 Secondary extract Example 2 25.25 49.81 Secondary extract Example 3 26.22 55.13 2nd extract (peel) Example 4 19.67 39.42 Primary extract Arbutin 10.02 31.31 Whitening cosmetic raw materials

As can be seen in Table 2, in the cell culture of melanoma cells that are melanin-producing cells, it can be seen that the rambutan extract according to the present invention has an excellent melanin production inhibitory effect. In addition, it showed superior inhibitory activity compared to arbutin used as a raw material of the whitening cosmetics, Examples 1 to 3 extracted by the extraction method according to the present invention compared to Example 4, the primary extract has excellent melanin inhibitory activity Seemed. On the other hand, Example 3, a rambutan fruit extract, exhibited better melanin inhibitory activity than Examples 1 to 2, a rambutan fruit extract.

Experimental Example 3

(Whitening efficacy test by clinical experiment)

In order to test the clinical whitening efficacy of the whitening cosmetic composition of the present invention, Examples 1 to 5 and known to the 100 subjects (20 people per sample) who suffer from pigmentation such as blemishes, freckles on the face Arbutin, known as a whitening agent, was dissolved in a vehicle of propylene glycol / ethanol (8/2) at a concentration of 1% and applied to the spots of the face twice daily for three months, once every morning and evening.

After use, the degree of improvement of pigmentation was determined according to the following criteria, and the results are shown in Table 3.

* Evaluation criteria for whitening efficacy

 Excellent effect: Pigmentation is hardly noticeable.

With effect: Pigmentation is very soft.

Slightly effective: Pigmentation is softened.

No effect: no change.

TABLE 3

Example 1 Example 2 Example 3 Example 4 Example 5  Arbutin Whitening effect ◎ ◎ ◎ ○ ○ △  (Double-circle): The ratio which shows the effect excellent or the effect in a subject is 80% or more. ○: The ratio which shows the effect excellent or the effect in a subject is 50%-80%. (Triangle | delta): The ratio which shows the effect excellent or the effect in a subject is 30%- 50% ×: less than 30% of the subjects show excellent or effective effects

       As can be seen in Table 3, the rambutan extract of the present invention is more excellent in clinical whitening efficacy than arbutin, and the second extract by the extraction method according to the present invention than the primary extract Examples 4 to 5 It can be seen that 1 to 3 is more excellent clinical whitening efficacy.

In summation of the above Experimental Examples 1 to 3, it can be seen that the rambutan extract of the present invention reduces the melanin production by inhibiting the activity of tyrosinase involved in the skin process and at the same time inhibiting the melanin production process. In addition, it can be seen that the rambutan extract extracted by the extraction method according to the present invention is more excellent in inhibiting tyrosinase activity and inhibiting melanin production.

Based on the results of the above experimental examples, solubilized formulations and emulsified formulations which can provide the skin whitening effect by containing the rambutan extract of Example 1 are presented. However, the composition of the present invention is not limited only to the following preparation examples.

(Production example)

(Production Examples 1 to 3)

(Emulsification formulation)

TABLE 4

Raw material name Preparation Example 1 Preparation Example 2 Preparation Example 3 1. Stearic acid 0.1 0.1 0.1 2. Stealiaryl Alcohol Alcohol 0.4 0.4 0.4 3. Glyceryl Monostearate 1.2 1.2 1.2 4. Beeswax 0.5 0.5 0.5 5. Polyoxyethylene sorbitan monolauric acid ester 2.5 2.5 2.5 6. Methyl Paraoxybenzoate 0.1 0.1 0.1 7. Paraoxybenzoic Acid Profiles 0.05 0.05 0.05 8. Cetylethylhexanoate 2 2 2 9. Triglycerides 5 5 5 10. Cyclomethicone 5 5 5 11. Distilled Water to 100 to 100 to 100 12. Thickened glycerin 5 5 5 13. Triethanolamine 0.15 0.15 0.15 14. Polyacrylic Acid Polymer 0.12 0.12 0.12 15. Pigment 0.0001 0.0001 0.0001 16. Incense 0.1 0.1 0.1 17. Example 1 Extract 0.0001 One 10

 The manufacturing method of an emulsifier type is as follows.

1) The mixture of the raw materials of 1-9 was heated at 65-70 degreeC.

2) 10 raw materials were added to the mixture of 1) above.

3) The mixture of the raw materials of 11-13 was heated at 65-70 degreeC, and was completely dissolved.

4) The mixture of 2) was emulsified at 8,000 rpm for 2 to 3 minutes while the mixture of 2) was slowly added to the mixture of 3) while heating at 65 to 70 ° C.

5) The raw material of 14 was dissolved in a small amount of water, and then added to the mixture of 4) and emulsified for 2 minutes.

6) The raw materials of 15 to 17 were weighed, and then put into the mixture of 5) and emulsified for 30 seconds.

7) After the mixture of 6) was emulsified and degassed and cooled to 25 ~ 35 ℃, an emulsion was prepared.

(Production Examples 4 to 6)

Solubilized Formulation

TABLE 5

Raw material name  Preparation Example 4  Preparation Example 5  Preparation Example 6 1. Purified water  to 100  to 100  to 100 2. Concentrated glycerin  3  3  3 3. 1,3-butylene glycol  3  3  3 4.EDTA-2Na  0.01  0.01  0.01 5. Pigment  0.0002  0.0002  0.0002 6. Example 1 Extract  0.001  One  10 7. Alcohol (95%)  10  10 10 8. Methyl Paraoxybenzoate  0.1  0.1  0.1 9. Polyoxyethylene Hydrogenide Ester  0.2  0.2  0.2 10. Incense  0.1  0.1  0.1 11.cyclomethicone   -   -  0.1

The preparation of the solubilized formulation is as follows.

1) The raw materials of 2 to 6 were put into purified water of 1 above and dissolved using a still mixer.

2) The raw materials of 8 to 11 were put in the alcohol of 7 and completely dissolved.

3) The mixture of 2) was solubilized with slow addition to the mixture of 1).

 Experimental Example 4

(Stability test of formulation)

The storage stability as a cosmetic was evaluated for the formulations of Preparation Examples 1 to 6 prepared above. In order to confirm the storage stability, the stability of the temperature change was investigated, and the results are shown in Table 6.

[Table 6] Storage Stability by Temperature

Temperature ( ^o C) Preparation Example 1 Preparation Example 2 Preparation Example 3 Preparation Example 4 Preparation Example 5 Preparation Example 6  -5  1 week 1 month 3 months     ◎ ◎ ◎     ◎ ◎ ◎     ◎ ◎ ◎     ◎ ◎ ◎     ◎ ◎ ◎     ◎ ◎ ◎   5  1 week 1 month 3 months     ◎ ◎ ◎     ◎ ◎ ◎     ◎ ◎ ◎     ◎ ◎ ◎     ◎ ◎ ○     ◎ ◎ ◎  30  1 week 1 month 3 months     ◎ ◎ ◎     ◎ ◎ ◎     ◎ ◎ ◎     ◎ ◎ ○     ◎ ◎ ◎     ◎ ◎ ◎  37  1 week 1 month 3 months     ◎ ◎ ◎     ◎ ◎ ◎     ◎ ◎ ◎     ◎ ◎ ○     ◎ ◎ ○     ◎ ◎ ○  45  1 week 1 month 3 months     ◎ ◎ ◎     ◎ ◎ ○     ◎ ◎ ○     ◎ ◎ ○     ◎ ◎ ○     ◎ ◎ ○ O Very good: Very good,: Good, X: Separation

      From the results of Table 6, it can be seen that the formulation according to the present invention is excellent in storage stability.

  As described above, the whitening cosmetic composition containing the rambutan extract of the present invention is excellent in skin whitening effect by inhibiting tyrosinase involved in the skin blackening process without skin irritation and at the same time inhibiting melanin production.

In addition, the whitening cosmetic composition containing the rambutan extract of the present invention can be provided as an emulsified cosmetic formulation using water-soluble cosmetics or emulsification technology, such as softening longevity using solubilization technology, it is also excellent storage stability when formulated.

In addition, according to the production method of rambutan extract of the present invention can increase the content and safety of the active ingredient of the extract.

Claims (9)

      Whitening cosmetic composition containing a rambutan extract (Nephelium lappaceum L, var. Lappaceum). The content of the rambutan extract in claim 1, Whitening cosmetic composition, characterized in that 0.001 to 10% by weight based on the total weight of the cosmetic composition. The content of the rambutan extract in claim 1, Whitening cosmetic composition, characterized in that 0.01 to 5% by weight based on the total weight of the cosmetic composition. According to claim 1, The rambutan extract is a whitening cosmetic composition, characterized in that the rambutan rind extract. According to claim 1, The formulation of the cosmetic composition, Whitening cosmetic composition, characterized in that the flexible cosmetics, astringent cosmetics, nourishing cosmetics, eye cream, nourishing cream, massage cream, powder, essence, pack, ointment, spray, essence, consil stick.      (a) adding at least one of an extraction solvent consisting of anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms, a mixture of water and lower alcohols, acetone and 1,3-butylene glycol, to the rambutan dried product, and then Extracting by heating for 3 to 24 hours at 50 ~ 200 ℃ in the attached distillation apparatus; (b) aging the extract of step (a) for 1 to 10 days at 4 ~ 25 ℃; (c) the aging solution of step (b) is concentrated under reduced pressure at 50-90 ° C. in a distillation apparatus equipped with a cooling capacitor, and at least one selected from an extraction solvent consisting of ethyl acetate, diethyl ether and methylene chloride. Extracting; And, (d) concentrating and drying the organic layer in the extract of step (c) under reduced pressure; Rambutan extraction method comprising a. The method of claim 6, wherein step (d) Rambutan extraction method characterized in that the drying by using anhydrous sodium sulfate as a drying agent, concentrated under reduced pressure, lyophilization or spray drying. (a) adding at least one of an extraction solvent consisting of anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms, a mixture of water and lower alcohols, acetone and 1,3-butylene glycol, to the rambutan dried product, and then Extracting by heating to 50 ~ 200 ℃ for 3 to 24 hours in the attached distillation apparatus; (b) aging the extract of step (a) for 1 to 10 days at 4 ~ 25 ℃; (c) the aging solution of step (b) is concentrated under reduced pressure at 50-90 ° C. in a distillation apparatus equipped with a cooling capacitor, and at least one selected from an extraction solvent consisting of ethyl acetate, diethyl ether and methylene chloride. Extracting; And, (d) concentrating and drying the organic layer in the extract of step (c) under reduced pressure; Rambutan extract extracted by the rambutan extraction method comprising a. A whitening cosmetic composition containing the rambutan extract of claim 8.