KR20050081753A - Cosmetic composition for protecting skin comprising plant extract stabilized in nanoliposome - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin protection comprising a green leaf, baeknyeoncho, licorice and pine needle extract as an active ingredient, and a cosmetic composition for skin protection comprising nanoliposomes stabilized by inclusion of the extract. The cosmetic composition for protecting the skin of the present invention has excellent free radical scavenging activity, inhibiting skin cell death, inhibiting erythema production, anti-inflammatory action, and alleviating skin irritation. Moreover, the cosmetic composition containing the nanoliposomes of the present invention has excellent stability by nanoliposomes the above-mentioned coatings, baeknyeoncho, licorice and pine needle extract, the effective ingredients, excellent skin penetration and excellent effect of the effective ingredients to protect the skin Show efficacy.

Description

Cosmetic composition comprising a plant extract stabilized with nanoliposomes {Cosmetic Composition for Protecting Skin Comprising Plant Extract Stabilized in Nanoliposome}

The present invention relates to a cosmetic comprising a plant extract as an active ingredient, and more particularly, to a cosmetic composition for skin protection comprising a green leaf, baeknyeoncho, licorice and pine needle extract as an active ingredient and nanoliposomes stabilized by inclusion of the extract. It relates to a cosmetic composition for protecting the skin.

Skin, the largest organ that makes up the human body, has various physiological functions such as protecting various organs inside the skin from the outside and barrier function, temperature control function, excretion function and respiratory function to prevent the internal moisture and useful components from leaking out. It is a very important organization in charge. However, with age, the thickness of the epidermis, dermis, and subcutaneous tissue becomes thin, which lowers the skin barrier function and the skin's physiological function decreases due to a drastic decrease in the moisture content of the skin. In addition to aging according to age, modern people are exposed to harmful environment such as air pollution, uneven diet, UV exposure, stress, and disease, so that the skin is sufficiently protected from photoaging and oxidative stress caused by various radicals. It is difficult to do.

In particular, ultraviolet light induces the production of free radicals in the skin, the free radicals destroy the skin's antioxidant system, and continued oxidative stress damages skin cell components (e.g., lipids, proteins and nucleic acids, etc.) Causes skin irritation and skin irritation. Therefore, the development of a safe and effective material that protects the skin from oxidative stress caused by ultraviolet rays and free radicals and external harmful substances has emerged as an important task in the cosmetic field, and many studies have been conducted. In recent years, the use of natural ingredients in cosmetics has been increasing rapidly, and various skin physiological activities on natural plant ingredients have been searched for, and materials having excellent skin beauty effects have been developed.

However, most natural plant components are not only unstable by themselves, but when applied to cosmetics, they have a big problem in the stability of cosmetics such as precipitation of contents, color change, and the like. In order to solve this problem, pharmaceuticals, cosmetics and food fields, such as bioactive components can be delivered stably without destruction, and the liposomes can be contained whether the bioactive components are fat-soluble or water-soluble. Recently, with the development of nano technology, research and development on nano liposomes that can maximize the skin penetration effect is also active.

Accordingly, the present inventors have diligently researched how to find natural ingredients having excellent efficacy in protecting skin from oxidative stress caused by ultraviolet rays and free radicals and external harmful substances and use them more effectively. The pine needle extract has an excellent effect on skin protection, and when the extract is stabilized by inclusion in nanoliposomes, the present invention was confirmed by facilitating skin penetration of these active ingredients and solving stability in cosmetics.

Accordingly, it is an object of the present invention to provide a cosmetic composition for skin protection comprising green leaf, zinnia, licorice and pine needle extract as an active ingredient.

Another object of the present invention is to provide a cosmetic composition for skin protection comprising nanoliposomes stabilized by inclusion of green leaf, baeknyeoncho, licorice and pine needle extract.

Other objects and advantages of the present invention will become apparent from the following examples and claims.

According to an aspect of the present invention, the present invention provides a cosmetic composition for skin protection comprising a green leaf, baeknyeoncho, licorice and pine needle extract as an active ingredient.

The cosmetic composition of the present invention is characterized in that it comprises extracts of green leaf, zinnia, licorice and pine needles.

Foliage is the dried mouth of peach ( Prunus persica L.), and has been widely used in connection with skin diseases since ancient times. The leaf extract of the present invention is not only excellent in the antioxidant effect, but also has a characteristic of preventing the destruction of keratinocytes and fibroblasts inside the human body caused by ultraviolet rays, etc. Activation can be suppressed and the harmful action by ultraviolet irradiation can be prevented.

Centennial ( Opuntia ficus-indica var. Saboten Makino.) Has long been said to be effective in incurable diseases such as hemorrhoids, coughs, boils, burns, stomachaches, antipyretics and herbal medicines that help blood circulation in the human body. In addition, it is said that the effect is excellent in the treatment of skin diseases and burns. The early hundreds are rich in minerals and vitamin C. Vitamin C acts as a powerful bio-oxidant in skin, blood and other tissues. Vitamin C has a sugarlike structure that accepts two electrons and is easily oxidized in the form of dehydro-L-ascorbic acid, and is highly reactive free radicals and free radicals in vivo. It prevents the oxidization or degeneration of biological components (proteins, nucleic acids and cell membrane lipids) by radicals and peroxides and has an antioxidant effect that blocks the cause of skin aging. At the beginning of the twentieth year, vitamin C has a content of 0.16%, which is about 5 times higher than aloe on a building basis, and shows excellent antioxidant effect, and shows excellent effect when applied to skin beauty.

Licorice ( Glycyrrhiza glabra L.) is a perennial herb of the dicotyledonous plant Rosaceae, which is used to dry the roots of the plant. Licorice extract is known to be effective in relieving skin stimulation because it has excellent skin cell activity and regeneration potency, antioxidant activity, free radical scavenging and inhibitory effect on secretion of inflammatory mediators. The superiority of the stimulation alleviation of licorice extract has already been found, and the contents have been transmitted in the old literature, and the present inventors have already used licorice extensively in cosmetics for the skin irritation relief effect.

Pine needles are young shoots of the pine tree ( Pinus densiflora ), which contain a large amount of flavonoids and have free radical scavenging activity. Activated oxygen 1O ₂ (or OH) initiates a lipid peroxidation reaction and destroys the cell membrane through an automatic oxidation process. In addition, the lipid peroxide formed by 1O ₂ (or OH) may be oxidized or reduced to peroxyradical or alkoxyradical in the presence of metal ions such as Fe ²⁺ . Pine needle extract acts to terminate the chain reaction by reacting with these peroxyradicals involved in the chain reaction of automatic oxidation. In addition, it reacts with DPPH radical (1,1-Diphenyl-2-picrylhydrazyl radical), which is a stable free radical, to remove free radicals.

The extracts of the green leaf, zinnia, licorice and pine needles of the present invention can be obtained using various extraction methods known in the art, and preferably, (a) water, (b) anhydrous or lower water content of 1-4 carbon atoms. Alcohols (methanol, ethanol, propanol, butanol, etc.), (c) a mixed solvent of the lower alcohols with water, (d) acetone, (e) ethyl acetate, (f) chloroform or (g) 1,3-butylene Glycol can be obtained as an extraction solvent.

On the other hand, it will be apparent to those skilled in the art that the extract of the present invention can be obtained not only the above-described extraction solvent but also herbal extracts having substantially the same effect using other extraction solvents.

With reference to the following specific preparation of the present invention, the preparation method of the powder daco is described as follows: dried and then powdered green leaf, baeknyeoncho, licorice and pine needles are put in aqueous ethanol aqueous solution and extracted at a constant temperature. Filter with a filter cloth, aged at low temperature and filtered. The extract is concentrated under reduced pressure and dried.

In addition, the extract of the present invention includes not only the extract by the above-described extraction solvent, but also the extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also included in the herbal extract of the present invention.

The herbal extract of the present invention may be prepared in a powder state by an additional process such as vacuum distillation and freeze drying or spray drying.

According to a preferred embodiment of the present invention, the content of the plant extract is 0.001% to 30%, respectively, based on the total weight of the composition.

Cosmetic composition comprising the green leaf, zinnia, licorice and pine needle extract of the present invention as an active ingredient has the use of skin protection.

As used herein, the term "skin protection" refers to the prevention, alleviation and treatment of irritation such as itching, tingling, burning, etc. and inflammatory stimuli such as erythema and edema appearing on the skin by skin irritants.

Green leaf, zinnia, licorice and pine needle extract of the present invention has an excellent free radical scavenging activity and has the effect of inhibiting the death of skin cells by free radicals. In addition, it inhibits and alleviates erythema, skin inflammation, and skin irritation that may be caused from external harmful substances such as ultraviolet rays, and this fact is specified in the following examples. Thus, skin protection as a use of the present invention includes the use of erythema suppression, skin inflammation improvement and skin irritation relief.

According to another aspect of the present invention, the present invention provides a cosmetic composition for skin protection comprising nanoliposomes stabilized by inclusion of green leaf, baeknyeoncho, licorice and pine needle extract.

The cosmetic of the present invention is characterized in that it comprises nanoliposomes stabilized by inclusion of a plant extract.

In a preferred embodiment of the present invention, the content of the green leaf, zinnia, licorice and pine needle extract is 0.1-20.0% by weight and preferably 1.0-10.0% by weight, respectively.

In a preferred embodiment of the present invention, the content of the nanoliposomes is contained 0.01-20.0% by weight relative to the total weight of the cosmetic composition, preferably 0.1-10.0% by weight.

As used herein, the term "nanoliposomes" refers to liposomes having an average particle diameter of 1-100 nm as having the form of conventional liposomes. According to a preferred embodiment of the present invention, the average particle diameter of the nanoliposomes is 30-100 nm. When the average particle diameter of the nanoliposomes exceeds 100 nm, the improvement of skin penetration and the improvement of formulation stability are very weak among the technical effects to be achieved in the present invention.

The nanoliposomes used to encapsulate the green leaf, zinnia, licorice and pine needle extracts of the present invention are prepared by a mixture comprising polyols, oily ingredients, surfactants, phospholipids and water, using monohydric alcohols such as ethanol. It is characterized by not.

The polyol used in the nanoliposomes of the present invention is not particularly limited, preferably propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, methylpropanediol, isopropylene glycol, Pentylene glycol, erythritol, xylitol, sorbitol and mixtures thereof. The amount used is 10-80% by weight, preferably 30-70% by weight based on the total weight of the nanoliposomes.

The oil component used in the preparation of the nanoliposome of the present invention may be various oils known in the art, preferably hydrocarbon-based oils such as hexadecane and paraffin oil; Synthetic synthetic oils; Silicone oils such as dimethicone and cyclomethicone series; Animal and vegetable oils such as sunflower oil, corn oil, soybean oil, avocado oil, sesame oil and fish oil; Ethoxylated alkyl ether oils; Propoxylated alkyl ether oils; Sphingoidoid lipids such as phytosphingosine, sphingosine and sphinginine; Cerebroside; cholesterol; Cytosterol; Cholesteryl sulfate; Cytosteryl sulfate; C10-40 fatty alcohols and mixtures thereof. The amount used is 1.0-30.0% by weight, preferably 3.0-20.0% by weight based on the total weight of nanoliposomes.

Surfactants used in the preparation of the nanoliposomes of the present invention may be any known in the art, for example, anionic surfactants, cationic surfactants, amphoteric surfactants and nonionic surfactants may be used. , Preferably anionic surfactants and nonionic surfactants are used. Specific examples of anionic surfactants include alkylacylglutamate, alkylphosphates, alkyllactylates, dialkylphosphates and trialkylphosphates. Specific examples of nonionic surfactants include alkoxylated alkylethers, alkoxylated alkylesters, alkylpolyglycosides, polyglyceryl esters and sugar esters. Most preferably, polysorbates belonging to the nonionic surfactant are used. The amount of the surfactant to be used is generally 0.1-10% by weight, preferably 0.5-5.0% by weight based on the total weight of the nanoliposomes.

Phospholipids, another component used in the preparation of the nanoliposomes of the present invention, are used as amphoteric lipids, including natural phospholipids (eg, egg yolk lecithin or soy lecithin, sphingomyelin) and synthetic phospholipids (eg dipalmitoyl). Phosphatidylcholine or hydrogenated lecithin), preferably lecithin. More preferably, the lecithin is a naturally occurring unsaturated or saturated lecithin extracted from soybean or egg yolk. Typically, lecithin derived from nature has 23-95% phosphatidylcholine and 20% or less phosphadidylethanolamine. In preparing the nanoliposomes of the present invention, the amount of lecithin used is 0.5-20.0% by weight, and preferably 2.0-8.0% by weight, based on the total weight of the nanoliposomes.

Water used for the preparation of the nanoliposomes of the present invention is generally deionized distilled water, the amount of which is used is 5.0-40% by weight relative to the total weight of nanoliposomes.

Fatty acids may be additionally used in the preparation of the nanoliposomes of the present invention. The fatty acids used in the preparation of the nanoliposomes of the present invention are higher fatty acids, preferably saturated or unsaturated fatty acids of C _12-22 alkyl chains such as lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid and linoleic acid. It includes. The amount used is 0.05-3.0 wt% with respect to the total weight of nanoliposomes, preferably 0.1-1.0 wt%.

The preparation of nanoliposomes can be accomplished through various methods known in the art, but most preferably, a mixture comprising the above components is applied to a high pressure homogenizer. The preparation of nanoliposomes by high pressure homogenizer can be carried out under various conditions (eg pressure, frequency, etc.) depending on the desired particle size, preferably 1-5 times high pressure homogenizer under 600-1200 bar pressure. Nanoliposomes are prepared by passing through.

The components included in the cosmetic composition of the present invention include components conventionally used in cosmetic compositions in addition to nanoliposomes including green leaf, zinnia, licorice and pine needle extract as active ingredients, such as stabilizers, solubilizers, vitamins, pigments and Conventional adjuvants such as perfumes, and carriers.

The skin care cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, containing solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, and surfactants. It may be formulated by cleansing, etc., but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack.

When the formulation of the present invention is a paste, cream or gel, carrier oils include animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silica, talc or zinc oxide. Can be used.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol , Fatty acid esters of 1,3-butylglycol, oils, glycerol aliphatic esters, polyethylene glycols or sorbitan.

When the formulation of the present invention is a suspension, a carrier diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide ether. Sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

According to the present invention, the use of green leaf, zinnia, licorice and pine needle extract in combination with nanoliposomes exhibits more improved skin inflammation and skin irritation effect.

The inclusion of green leaf, zinnia, licorice and pine needle extract in nanoliposomes greatly increases the skin penetration effect and greatly improves the stability of the formulation. Therefore, the cosmetic composition of the present invention comprising nanoliposomes encapsulated with green leaf, zinnia, licorice and pine needle extract provides an excellent effect on the inhibition of erythema production by ultraviolet rays, improving skin inflammation and alleviating skin irritation.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it is to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. Will be self-evident.

Example

Preparation of Extract

After washing with purified water and drying, 1 kg of powdered leaf powder, 1 kg of baeknyeoncho powder, 1 kg of licorice powder and 1 kg of pine needle powder were added to 5 L of 70% ethanol aqueous solution and extracted at 15-40 ° C. for 5 days. Filtration was carried out with a 300 mesh filter cloth, left at 4-15 ° C. for 10 days, and then aged at low temperature, followed by filtration using Whatman No. 2 filter paper. The extract was concentrated under reduced pressure at 70 ° C. in a distillation apparatus with a cooling condenser and dried to obtain 152 g (dry weight), and the extraction yield was 3.8 ± 0.5%.

Experimental Example 1: Free radical scavenging effect

In order to examine the free radical scavenging effect on the plant mixture extract of the present invention, DPPH (1,1-diphenyl-2-picrylhydrazy radical) solution (60M) (Sigma-Aldrich, USA) was prepared. The plant mixture extract was then adjusted by concentration (10, 20, 50 and 100 μg / ml), and DPPH solution was mixed therein at a 1: 1 ratio and then vortexed. After 30 minutes of reaction at 20 ℃ absorbance was measured at 520 nm. The free radical scavenging effect was calculated according to Equation 1, and the results are shown in Table 1 below. The control group used ethanol instead of the sample.

Free radical scavenging effect (%) = 100- (absorbance after extract treatment / control absorbance) x100

Extract concentration (µg / ml) Free radical scavenging effect (%) 10 42.8 20 71.4 50 79.2 100 91.8

Looking at the Table 1, it can be seen that the plant mixture extracts of green leaf, baeknyeoncho, licorice and pine needles show excellent free radical scavenging effect.

Experimental Example 2: H ₂ O ₂ Inhibitory effect of human keratinocyte death by

When ultraviolet rays are irradiated to keratinocytes, active oxygen is secreted into the cells. To induce this artificially, 0.05 mM of H ₂ O ₂ was added to the culture medium to affect keratinocytes (Korea Cell Line Bank). After 4 hours after adding the plant mixture extract (10, 20, 50, 100 ㎍ / ㎖), the cell activity was confirmed by MTT analysis.

Extract concentration (µg / ml) Cell survival rate (%) 0* 12.5 10 46.6 20 57.8 50 66.7 100 77.3

* H ₂ O ₂ 0.05 mM treated cells

Referring to Table 2, the plant mixture extracts of green leaf, zinnia, licorice and pine needles showed an excellent effect of inhibiting apoptosis by hydrogen peroxide in a concentration-dependent manner.

Experimental Example 3: H ₂ O ₂ Inhibitory Effect of Human Fibroblasts by

When ultraviolet rays are irradiated to fibroblasts, free radicals are secreted in the cells. To induce this artificially, 0.05 mM of H ₂ O ₂ was added to the culture medium to affect fibroblasts (Korea Cell Line Bank). After 4 hours after adding the plant mixture extract (10, 20, 50, 100 ㎍ / ㎖), the cell activity was confirmed by MTT analysis.

Extract concentration (µg / ml) Cell survival rate (%) 0* 12.5 10 43.6 20 60.8 50 69.7 100 80.3

* H ₂ O ₂ 0.05 mM treated cells

Looking at the Table 3, the extracts of plants, baeknyeoncho, licorice and pine needles showed an excellent effect of inhibiting cell death by hydrogen peroxide in a concentration-dependent manner.

Experimental Example 4: Preparation of Nanoliposomes Containing Plant Mixture Extract

The nanoliposomes were added to phase A as the composition of Table 4 below, wetted uniformly, and then phase C was added to AB and heated and mixed to 70-77 ° C., followed by uniform stirring. Subsequently, the mixture was passed through a high-pressure homogenizer 1-5 times under conditions of 600-1,200 bar and cooled to prepare nanoliposomes having a particle size of 30-100 nm in diameter.

Prize ingredient Content (% by weight) Production Example-1 Preparation Example-2 Preparation Example-3 Preparation Example-4 Preparation Example-5 Preparation Example-6 Preparation Example-7 Preparation Example-8 Preparation Example-9 Preparation Example-10 A glycerin 60.0 0.0 0.0 0.0 0.0 30.0 0.0 30.0 0.0 0.0 1,3-butylene glycol 0.0 60.0 0.0 60.0 0.0 30.0 30.0 0.0 60.0 0.0 Propylene glycol 0.0 0.0 60.0 0.0 60.0 0.0 30.0 30.0 0.0 60.0 B lecithin 5.0 5.0 5.0 7.0 0.0 0.0 0.0 6.0 3.0 3.0 Immersion Lecithin 0.0 0.0 0.0 0.0 6.0 6.0 6.0 0.0 3.0 3.0 cholesterol 0.0 0.0 2.0 1.0 1.0 0.0 0.0 0.0 1.0 1.0 Ceramide 0.0 2.0 0.0 1.0 1.0 0.0 2.0 2.0 1.0 1.0 Stearic acid 2.0 2.0 2.0 1.0 0.0 0.0 2.0 2.0 2.0 1.0 Palmitic Oleic Acid 0.0 0.0 0.0 1.0 2.0 2.0 0.0 0.0 1.0 2.0 Dimethicone 5.0 5.0 5.0 5.0 0.0 0.0 5.0 5.0 5.0 8.0 Jojoba oil 0.0 0.0 0.0 0.0 0.0 2.0 0.0 5.0 0.0 0.0 Squalane 0.0 0.0 0.0 0.0 10.0 0.0 5.0 0.0 0.0 0.0 Alkyl phosphate 0.5 1.0 0.5 0.5 0.5 0.0 1.5 0.0 0.0 0.5 Alkyl lactylates 0.5 0.0 1.0 0.5 0.0 1.5 0.0 0.0 1.0 0.5 Dialkyl Phosphate 0.5 0.5 0.0 0.0 1.5 0.0 0.0 1.5 0.0 0.0 C Plant mix extract 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 Potassium hydroxide Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity Purified water To.100 To.100 To.100 To.100 To.100 To.100 To.100 To.100 To.100 To.100

Examples and Comparative Examples

Compositions containing nanoliposomes stabilized by inclusion of the green leaf, zinnia, licorice and pine needle extract of the present invention (Example) and compositions without nanoliposomes (Comparative Example) were prepared in the following compositions.

ingredient Example 1 Example 2 Comparative Example 1 Content (% by weight) Content (% by weight) Content (% by weight) Nanoliposomes (Production Example-6) 10.0 - - Plant mix extract - 1.0 - A Cetostearyl alcohol 2.0 2.0 2.0 Glyceryl Stearate 1.5 1.5 1.5 Microcrystalline Wax 0.7 0.7 0.7 Squalane 5.0 5.0 5.0 Liquid paraffin 3.0 3.0 3.0 Trioctanoine 5.0 5.0 5.0 Polysorbate 60 1.2 1.2 1.2 Sorbitan stearate 0.5 0.5 0.5 Tocopheryl Acetate 0.2 0.2 0.2 Cyclomethicone 3.0 3.0 3.0 BHT 0.05 0.05 0.05 B glycerin 4.0 4.0 4.0 1,3-butylene glycol 2.0 2.0 2.0 EDTA-2Na 0.05 0.05 0.05 Purified water To 100 To 100 To 100 Incense, preservative Quantity Quantity Quantity

Experimental Example 5: Inhibition of erythema formation upon ultraviolet irradiation

In order to determine the inhibitory effect of erythema formation upon ultraviolet irradiation, cosmetics containing nanoliposomes stabilized by inclusion of the green leaf, zinnia herb, licorice and pine needle extract of the present invention using a color difference meter (manufactured by Minolta, Chromameter CR 300) The value was calculated.

Thirty healthy women (mean age 29.5 years old) were divided into three groups of 10 people indoors, and UV light was irradiated to the upper arm of the subject at 0.88 J / ㎠, three times per day. Samples according to Examples 1, 2 and Comparative Example 1 were applied to each group twice a day for 30 days, and the Δa value which is the difference between the a value before application and the value of a with time after application. Was calculated according to Equation 2 below to compare the erythema production inhibitory effect, the results are shown in Table 6.

Efficacy inhibitory effect (%) = (a value before application-a value after application) / a value before application x 100

division Inhibition of erythema production (%) Example 1 92.0 Example 2 73.0 Comparative Example 1 4.0

As can be seen in Table 6, Example 2 containing a mixed extract of the green leaf, baeknyeoncho, licorice and pine needles of the present invention showed an excellent effect of inhibiting erythema formation by ultraviolet light compared to Comparative Example 1.

On the other hand, Example 1 containing the nano-liposomes stabilized by inclusion of the mixed extract was 19% increased erythema suppression effect compared to Example 2. Therefore, it can be seen that when the green leaf, baeknyeoncho, licorice and pine needle extract according to the present invention are included in the nanoliposome and stabilized, the erythema formation inhibitory effect is significantly improved.

Experimental Example 6: Anti-inflammatory effect evaluation method-Carrageenan foot edema

In order to confirm whether the cosmetic of the present invention exhibits an excellent anti-inflammatory effect, 1 hour after administering 10-100 mg / kg of Example 1, Example 2 and Comparative Example 1 to the mouse abdominal cavity, 0.1% carrageenan solution ( 0.5 ml of Sigma-Aldrich, USA) was injected into the hind paws of the experimental animals to cause inflammation. Inhibition rate was calculated according to the following Equation 3 by measuring the change in the volume of the mouse immediately after carrageenan injection and 4 hours after the injection, and the results are shown in Table 7.

% Inhibition = (1-ΔV treated group / ΔV control) x 100 (ΔV: change in foot volume)

Dose (mg) % Inhibition Rate Example 1 Example 2 Comparative Example 1 10 21.9 10.6 2.6 30 31.2 19.7 5.8 50 48.2 36.6 9.3 100 57.9 41.4 10.2

As can be seen in Table 7, Example 2 containing the mixed extract of the green leaf, baeknyeoncho, licorice and pine needles of the present invention showed an excellent anti-inflammatory effect compared to Comparative Example 1.

On the other hand, in the case of Example 1 containing the nano-liposomes stabilized by inclusion of the mixed extract was significantly inhibited compared to Example 2. Accordingly, it can be seen that the inclusion of green leaf, zinnia, licorice, and pine needle extract according to the present invention in the nanoliposomes and stabilizing the extract has an improved anti-inflammatory effect.

Experimental Example 7: Human skin patch test

In the past, the skin irritation effect was confirmed in human skin patch test of 20 women in their 20s and 30s who had no hypersensitivity reaction to skin irritation and have no skin disease or skin allergy symptoms. Example 1, Example 2, and Comparative Example 1 were used in which 5.0% of lactic acid, a skin irritant, was added as an irritant. First the test site was wiped with 70% ethanol and dried. 15 μg of the prepared test substance was dropped in a fin chamber (Finn chamber, 100 × 10, EPITEST, Finland), and then sealed in an inner portion of the subject's forearm. The test site was marked with a marking pen after patching for 24 hours and removing the patch. 1 hour and 24 hours after marking, the test site was observed using a magnifying glass (8MC-150, DAZOR, United States of America) to observe erythema and edema. Skin reactions were determined according to the regulations of the International Contact Dermatitis Research Group (ICDRG) and the results are shown in Table 8.

* Evaluation criteria and score of skin reaction

Symbol Score Criteria

  -0 no response

  ± 0.5 faint or light erythema

  + 1 clear but weak erythema, edema and papules

 ++ 2 distinct erythema, papules and vesicles

 +++ 3 Severe erythema and alveolar, scleroderma

Mean response rate = (score x responses x 100 x 1/2) / [3 (maximum score) x total number of subjects (n)]

Test substance After 1 hour After 24 hours % Reactivity (n = 20)  ± + ++ ± + ++ Example 1 + 5% Lactic Acid Example 2 + 5% Lactic Acid Comparative Example 1 + 5% Lactic Acid 6 0 -7 1 -9 3- 1 0 -2 0 -2 1- 2.924.587.92

As can be seen in Table 8, Example 2 containing a mixed extract of the green leaf, baeknyeoncho, licorice and pine needles of the present invention was found to have an excellent skin irritation-releasing effect compared to Comparative Example 1.

On the other hand, Example 1 containing the nano-liposomes stabilized by inclusion of the mixed extract was found to have a significantly improved skin irritation relief effect compared to Example 2.

Experimental Example 8: Comparison of skin irritation relaxing effect in human application

20 women in their 20s and 30s were allowed to use the cosmetic preparations of Examples 1, 2 and Comparative Example 1 containing 5.0% of the skin irritant lactic acid for 10 days. Each morning and evening were used, and then questionnaires were marked for irritation and intensity. The test results were evaluated once a day, and divided into subjective and objective stimuli, and the results are shown in Table 9 below.

division Subjective stimulus (% relaxation) Objective stimulation (% relaxation) Average (% relaxation) Example 1 49 62 55 Example 2 24 29 26 Comparative Example 1 7 11 9

As can be seen in Table 9, Example 2 containing a mixed extract of the green leaf, baeknyeoncho, licorice and pine needles of the present invention showed an excellent effect in both subjective and objective skin irritation relief compared to Comparative Example 1.

On the other hand, Example 1 containing the nano-liposomes stabilized by inclusion of the mixed extract was found to have a significantly improved skin stimulating effect compared to Example 2.

Experimental Example 9: Skin Penetration Effect

The skin penetration effect on the cosmetic containing nanoliposomes stabilized by inclusion of the green leaf, baeknyeoncho, licorice and pine needle extract of the present invention was measured.

Dermal Equivalent (DE)-Human Normal Fibroblasts 1x10 ⁵ cells / ml (Korea Cell Line Bank) in a gel structure similar to the fibrous tissue that forms the connective tissue of the human body 3 mg / ml: 5X DMEM: 2: 2 0.05 N sodium hydroxide containing sodium bicarbonate and 200 mM Hepes buffer was inoculated in a medium mixed with 7: 2: 1 and incubated at 5% CO ₂ , 37 ° C. for 7 days. The culture was placed inside a plate separated from the inside and the outside by a membrane made of 3 μm porous polycarbonate, and epidermal keratinocyte 1X10 ⁵ cell on the skin equivalent (DE) surface on which fibroblasts were cultured. / Ml inoculated with K-SFM medium (Sigma-Aldrich, USA) containing EGF and BPE inside and outside were incubated for 7 days. Subsequently, discard the medium inside the plate so that the air contacts the surface of the cultured cells, and incubate for 2 weeks in a medium mixed with the same amount of K-SFM containing 10% FBS and DMEM without EGF. Artificial skin with epidermis and dermis was obtained. Example 1 and Example 2 of the present invention was applied to the artificial skin tissue of the top layer, followed by 4 hours of incubation in a medium in which K-SFM containing 10% FBS and DMEM without EGF were mixed in the same amount. The amount of extract that penetrated the epidermis and dermis of artificial skin was analyzed by HPLC. The experimental results are shown in Table 10 below.

Amount of Plant Mixture Extract in Medium (µg / mL) Example 1 Example 2 39.6 3.8

As can be seen in Table 10, the cosmetic containing nanoliposomes stabilized by inclusion of the green leaf, baeknyeoncho, licorice and pine needle extract of the present invention 10 times or more compared to Example 2 using the green leaf, baeknyeoncho, licorice and pine needle extract as it is It was found that the skin penetration effect is excellent. Increasing the skin penetration can maximize the efficacy of the active ingredient, in Experimental Example 1 and Experimental Example 2, it can be seen that Example 1 shows an excellent effect than Example 2.

Experimental Example 10: Stability Test of the Formulation

The stability test for the cosmetics containing the nanoliposomes stabilized by inclusion of the green leaf, baeknyeoncho, licorice and pine needle extract of the present invention was carried out by the following method. In order to test the stability of the cosmetic Example 1 and Example 2 and Comparative Example 1 in a opaque glass container kept in a constant temperature maintained at 45 ℃ constant storage for 12 weeks and also kept completely at 4 ℃ After storing for 12 weeks in an opaque glass jar in the refrigerator, the discoloration, odor and separation of the product was measured. The results are shown in Table 11 below. At this time, the degree of discoloration, odor and separation of the product was evaluated by classifying into the following six grades.

* Standards for evaluation of discoloration, odor

0: No change 1: Very slight discoloration, odor, separation

2: a little discoloration, discoloration, separation 3: little discoloration, discoloration, separation

4: severely discolored, discolored, separated 5: extremely severely discolored, discolored, separated

division Product stability Example 1 Example 2 Comparative Example 2 45 ℃ 4 ℃ 45 ℃ 4 ℃ 45 ℃ 4 ℃ discoloration 0.6 0 3.1 0 0 0 Deodorant 0.2 0 2.6 0 0 0 detach 0.8 0 3.7 0 0 0

As can be seen from Table 11, Example 1 and Comparative Example 1 was stable at 45 ℃ without discoloration or separation symptoms, but Example 2 used directly in the formulation without stabilizing the green leaf, zinnia, licorice and pine needle extract of the present invention It can be seen that the extract is unstable due to severely separated and discolored at 45 ℃.

Hereinafter, based on the results of the above experimental example, to present a cosmetic composition containing a nanoliposome stabilized by inclusion of the green leaf, baeknyeoncho, licorice and pine needle extract of the present invention. However, the composition of the present invention is not intended to be limited to the following formulation examples.

Formulation Example 1: Softener (Skin Lotion)

As shown in Table 12 below, the flexible makeup was prepared according to a conventional method.

Compounding ingredient Content (% by weight) Nano liposome 3.0 1.3-butylene glycol 6.0 glycerin 4.0 EDTA-2Na 0.02 Sodium Hiruronate 2.0 Fiji-40 Hydrogenated Castor Oil 0.5 ethanol 15.0 Benzophenone-9 0.05 Incense, preservative a very small amount Purified water to 100

Formulation Example 2: Nutrients (Milk Lotion)

As shown in Table 13, nutrient lotion was prepared according to a conventional method.

Compounding ingredient Content (% by weight) Nano liposome 5.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 1.2 Tocophenyl Acetate 3.0 Liquid paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxyvinyl Polymer 0.3 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water to 100

Formulation Example 3: Nutrition Cream

As shown in Table 14, nutrition cream was prepared according to a conventional method.

Compounding ingredient Content (% by weight) Nano liposome 15.0 Cetostearyl alcohol 2.5 Glyceryl Stearate 1.5 Trioctanoine 5.0 Polysorbate 60 1.2 Sorbitan stearate 0.5 Squalane 5.0 Floating paraffin 3.0 Cyclomethicone 3.0 BH 0.05 Delta-tocopherol 0.2 Concentrated glycerin 4.0 1,3-butylene glycol 2.0 Santa sword 0.2 EDTA-2Na 0.05 Incense, preservative a very small amount Purified water to 100

Formulation Example 4: Massage Cream

Massage creams were prepared according to a conventional method as shown in Table 15 below.

Compounding ingredient Content (% by weight) Nano liposome 5.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Beeswax 2.0 Tocopheryl Acetate 0.1 Polysorbate 60 3.0 Sorbitan sesquioleate 2.5 Cetearyl Alcohol 2.0 Liquid paraffin 30.0 Carboxy Vinyl Polymer 0.5 Incense, preservative a very small amount Purified water to 100

Formulation Example 5: Pack

Packs were prepared according to conventional methods as shown in Table 16 below.

Compounding ingredient Content (% by weight) Nano liposome 2.0 Propylene glycol 2.0 glycerin 4.0 Carboxyvinyl Polymer 0.3 ethanol 7.0 Fiji-40 Hydrogenated Castor Oil 0.8 Triethanolamine 0.3 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water to 100

As described in detail above, the present invention relates to a skin protective cosmetic composition comprising a green leaf, baeknyeoncho, licorice and pine needle extract as an active ingredient and a skin protective cosmetic composition comprising a nanoliposome stabilized by inclusion of the extract. The cosmetic composition for protecting the skin of the present invention has excellent free radical scavenging activity, inhibiting skin cell death, inhibiting erythema production, anti-inflammatory action, and alleviating skin irritation. Furthermore, the cosmetic composition containing the nanoliposomes of the present invention has excellent stability by nanoliposomes the above-mentioned green leaf, baeknyeoncho, licorice and pine needle extract, and excellent skin penetration, so the effect of the active ingredient is maximized, very excellent skin protection Show efficacy.

Claims (14)

Cosmetic composition for skin protection, including green leaf, baeknyeoncho, licorice and pine needle extract as an active ingredient. According to claim 1, wherein the extract is a cosmetic composition for skin protection, characterized in that contained 0.001-30% by weight relative to the total weight of the composition. Cosmetic composition for skin protection comprising nanoliposomes stabilized by inclusion of the green leaf, baeknyeoncho, licorice and pine needle extract. According to claim 3, wherein the extract is a skin protective cosmetic composition, characterized in that contained 0.1-20.0% by weight relative to the total weight of the nanoliposomes. The cosmetic composition for protecting skin of claim 3, wherein the content of the nanoliposome is contained in an amount of 0.01-20.0 wt% based on the total weight of the composition. The cosmetic composition for skin protection according to claim 3, wherein the average particle diameter of the nanoliposomes is 30-100 nm or less. The cosmetic composition for protecting skin according to claim 3, wherein the nanoliposomes are prepared by a mixture comprising a polyol, an oily component, a surfactant, a phospholipid, and water. The method of claim 7, wherein the polyol is selected from the group consisting of propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, methylpropanediol, isoprene glycol, pentylene glycol, erythritol, ziitol and sorbitol The content of the cosmetic composition for skin protection, characterized in that 10.0-80.0% by weight based on the total weight of nanoliposomes. The method of claim 7, wherein the oil component is a hydrocarbon-based oil; Synthetic synthetic oils; Silicone oils; Animal and vegetable oils; Ethoxylated alkyl ether oils; Propoxylated alkyl ether oils; Sphingoidoid lipids; Cerebroside; cholesterol; Cytosterol; Cholesteryl sulfate; Cytosteryl sulfate; C ₁₀ - ₄₀ fatty alcohols, and is selected from the group consisting of mixtures thereof, in amounts of protective skin cosmetic composition, characterized in that 1.0 to 30.0 wt% based on the total weight nanoliposome. The method of claim 7, wherein the surfactant is an anionic surfactant or alkoxylated alkyl ether, alkoxylated alkyl ester selected from the group consisting of alkylacyl glutamate, alkyl phosphate, alkyl lactylate, dialkyl phosphate and trialkyl phosphate, A nonionic surfactant selected from the group consisting of alkyl polyglycosides, polyglyceryl esters and sugar esters, the content of which is 0.1-10.0% by weight based on the total weight of nanoliposomes. According to claim 7, wherein the phospholipid is a natural phospholipid or synthetic phospholipid, the content of the cosmetic composition for protecting the skin, characterized in that 0.5-20.0% by weight relative to the total weight of the nanoliposomes. The cosmetic composition for skin protection according to claim 11, wherein the phospholipid is a naturally derived unsaturated or saturated lecithin. According to claim 3, wherein the nano liposome is a cosmetic composition for protecting the skin, characterized in that the mixture is prepared by applying a high pressure homogenizer. 3. The cosmetic composition according to claim 1 or 2, wherein the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation And a formulation selected from the group consisting of sprays.