KR200303608Y1 - Sample Holders for Cryo Preservating Vessel for Biological materials - Google Patents

Abstract

The present invention relates to a sample holder for a cryogenic cryopreservation vessel of a biological material, and more particularly, to a holder for a biological sample used in a vessel for cryogenic preservation of a biological sample.

The present invention is to be stored in a chamber of a double nitrogen tank (Liquid nitrogen Tank) in which the cryogenic material such as liquid nitrogen is contained therein; It consists of a case having a unit standard and a rack in which a plurality of cases are housed together with the case.

The present invention according to the above configuration is easy to handle a large amount of samples, such as blood bone marrow, and properly stored and stored, and further by specifying the sample to be stored by unit to prevent contamination or difficulty in handling between the samples, in particular It is an effective and useful design to provide a mechanism to prevent damage or contamination during transportation.

Description

Sample Holders for Cryo Preservating Vessel for Biological Materials

The present invention relates to a sample holder for a cryogenic cryopreservation vessel of a biological material, and more particularly, to a holder for a biological sample used in a vessel for cryogenic preservation of a biological sample.

Biological materials such as blood, plasma, lymph, semen, vaginal fluid, tissues or organs or fragments thereof, microbial cells, DNA or RNA, vectors, hybridomas, bone marrow cells, cancer cells, etc. If left unchanged, it changes over time and deteriorates.

The most effective way to stop the degradation process in these biological materials is known to freeze the biological materials and store them at cryogenic temperatures. Such cryopreservation is currently widely used in the biological and biomedical fields.

Cooling and freezing of biological materials is a very complex process involving chemical and physiological changes, and the effects of these changes on biological materials are known to depend on the structural complexity of the biological materials.

Although biological materials can be stored by some other means besides cryopreservation, cryogenic preservation is the only preservation method that can minimize changes in biological materials.

Cryopreservation is a preservation technology for biological materials that was proposed over 50 years ago and has been in use for decades. The most effective and easy way to bring the preservation system into a cold environment is to deposit it in liquid nitrogen to ensure the stability of the biological material. I can make it.

The most important factor in this cryopreservation method is to maintain the cryopreservation system in a constant temperature range below the minimum critical threshold temperature of the biological material, thereby impairing or gradual changes in the properties of the biological material during storage. Occurring or restorative action may be prevented. For example, it is known that the critical temperature of living cells is -130 ° C or lower, and therefore, it is preferable to maintain the cells in a liquid nitrogen freezer at -150 ° C to -196 ° C.

In the cryopreservation method, it is generally a matter of safety, sample integrity, and cross contamination.

However, if the sample is directly stored in liquid nitrogen, if the sample container (vinyl pack or plastic container, etc.) is incompletely sealed, liquid nitrogen will penetrate into the sample and burst upon opening the pack or container, resulting in partial loss or contamination of the sample. There is the potential to cause. Therefore, a carrier containing a large number of samples or samples is usually stored frozen in a carrier contained in liquid nitrogen. The carrier is heavy to lift and the liquid nitrogen must be discharged from the carrier before taking out the sample. Can often lead to splashing of liquid nitrogen, resulting in potential freezing burns.

In addition, cases have been reported in which pathogen transfer occurs between samples stored in liquid nitrogen.

Therefore, proper maintenance manipulation and handling of the biological material in cold storage is absolute for maintaining the stability of the biological material, a typical embodiment for this is a liquid nitrogen (Liquid nitrogen), as shown as an example in FIG. A low temperature material such as liquid nitrogen 41 is contained in the chamber 4 of the tank and submerged in the liquid nitrogen 41; Carrier 5 consisting of a normal body 51 having a certain internal volume and only an upper portion thereof, a bar 52 extending long from an upper end of the body 51, and a lead 53 derived from the bar 52. ; Carrier) is used.

In addition, the carrier 5 distinguishes which samples are stored in the carrier 5 by attaching a colored tag to the lid 53 because the lid 53 is caught and stored on the lid of the tank. However, since many carriers (5) cannot be accommodated due to its structure, each carrier (5) is apt to be kept mixed with various samples, which is inconvenient for use / storage, and leads when taking out the carrier (5) from a tank. While holding the 53 and lifting it a little, the normal body 51 has come out of the liquid nitrogen and is held for a predetermined time (the time at which the liquid nitrogen buried in the normal body 51 completely flows), and then passes through the long bar 52. The carrier 51 should be lifted up to exit the neck of the tank up to the normal body 51. At this time, the handler only needs to lift the lid 53 so that the handling of the carrier 5 is not easy. -196 ℃) means splashing to the handler There was a problem that the enemy never caused injury (such as cooling burns).

The present invention solves the above-mentioned problems, so that a large amount of samples, such as blood bone marrow, can be easily and properly stored and stored, and further, by specifying the samples to be stored in units to prevent contamination or difficulty in handling between the samples. In particular, it is to provide a mechanism for preventing damage or contamination during transportation.

In order to solve the above problems, the present invention is to be stored in a chamber of a double sealed tank (Liquid nitrogen Tank) in which the cryogenic material such as liquid nitrogen is contained therein; It consists of a case having a unit standard and a rack in which a plurality of cases are housed together with the case.

1 is a configuration diagram of the present invention,

2 is an exploded view of the present invention configuration state,

3 is another configuration of the present invention,

4 is a state diagram used in the present invention,

Figure 5 is an illustration of a state of use of the prior art.

* Explanation of symbols for the main parts of the drawings

One; case

11; Body 111; Hinge grooves 112; Jam

12; Cover 121; Garder 122; Hole 123; Hinge

2; Rack

21; Frame 22; Partition 221; Latch

23; Handle 24; latch

3; Base

31; septum

4; Chamber

41; Liquid nitrogen

5; carrier

51; Generic 52; Bar 53; lead

Hereinafter, the structure of the present invention with reference to the drawings in detail.

1 is a schematic view of the subject innovation, and FIG. 2 is a development view of the subject innovation configuration.

The present invention is to be stored in a chamber 4 of a double nitrogen tank (Liquid nitrogen Tank) in which a cryogenic material such as liquid nitrogen 41 is contained therein, as shown in the drawing; A body 11 having a hinge hole 111 on a side end and a locking jaw 112 at an upper end thereof, and accommodating predetermined contents; And a head 121 formed at both sides to guide the side of the body 11 to the inside, a hole 122 bored at the center, and a hinge groove 111 of the body 11. A cover 12 having a hinge 123 and covering the body 11; It is characterized by consisting of a case (1) to be.

3 is another configuration of the present invention.

In the above configuration, the frame 21 is formed along the corner so that one side is opened as a long hexahedron; A partition 22 partitioning the elongated hexahedron to a height at which the case 1 can be slid; And a handle 23 attached to the upper surface of the elongated hexahedron.

In the above configuration, the clasp grooves 221 which are respectively drilled in the partition 31 and the opening of the upper surface of the rack 2, and the clasps through the respective clasp grooves (221, 221, ...) at the same time ( It is more preferable if it is characterized by including 24).

4 is a state diagram used in the present invention.

Effects of each component of the present invention made as described above will be described in detail with reference to FIG. 4.

According to one configuration of the present invention, there is a case (1) stored in a chamber (4) of a liquid nitrogen tank in which a cryogenic material such as liquid nitrogen (41) is contained therein. As will be described later, this is a unit unit, and the base 3 on the plate is installed so as to be exposed on the surface of the liquid nitrogen 41 contained in the chamber 4 of the tank, and a plurality of bases are placed on the base 3, In such a structure, the average temperature in the chamber 4 is about -196 ° C., so that the sample can be titrated for storage of the sample, and the case 1 has a structure as follows.

That is, the case 1 is a cube formed of a body 11 and a cover 12. First, the body 11 is provided with a hinge hole 111 having a long hole at a side end, and a locking jaw 112 at a top end thereof. The cover 12 includes a guide 121 formed at both sides to guide the side of the body 11 to the inside, a hole 122 drilled at a central portion thereof, and a cover of the body 11. The hinge groove 111 is provided with a hinge 123 to which its head is hung, and covers the body 11, so that the guards 121 formed on both sides of the cover 12 have a quantity of the body 11. Wrap the outer side, the locking jaw 112 of the body 11 is pressed so as not to open the cover 12, storing the sample inside the body 11 and close the cover 12, made of a hard material such as metal Since the sample is completely covered and stored inside the case 1 to be manufactured, the case 1 remains closed even when dropped or impacted during transportation / storage. It will not affect the sample at all.

And, although the cover 12 is pressed and supported by the locking step 112, the hinge groove 111 of the body 11 is a long hole and the hinge 123 protruding from both sides of the cover 12 is the hinge groove 111 Since the hinge 123 is rotated while sliding in the hinge groove 111, the hinge 123 slides a predetermined distance and rotates when the cover 12 comes out of the latching jaw 112 so that the hinge 123 is rotated at all. There is no problem in this case, since the distance between the two midpoints of the long hole forming the hinge groove 111 becomes the slide distance of the cover 12, so that there is no problem during operation when it is longer than the protruding length of the locking jaw 112, the protrusion of the hinge 123 The head is larger than the hinge groove 111 will not be eliminated.

In addition, since both sides of the body 11 are covered by the guard 121 of the cover 12, the sides of the body 11 can be easily opened and closed by lifting and rotating while holding and sliding both sides, but for more convenient handling, the cover ( The hole 122 is drilled in the center portion of the center 12 to allow the user to put the finger to the above-described operation so that the opening and closing of the cover 12 can be made easier. In addition, although the case 1 is completely sealed but not sealed, the outside air (cooled air in the chamber 4 by the liquid nitrogen 41) is sufficiently communicated, and the case 1 is made of, for example, stainless steel or SUS. Since the temperature of the cover 12 is almost the same as the temperature of the chamber 4 because it is made of a metal having good thermal conductivity, such as 40, the hole 122 allows direct communication between the chamber 4 and the inside of the case 1. The temperature difference between them And by, in addition to a sample to be resolved (BB) it is contained in the case (1) with the naked eye thus providing a greater convenience in handling.

According to another constitution of the present invention, in addition to the case 1, the case 1 further comprises a rack 2 in which a plurality of cases are accommodated, and the rack 2 is a long hexahedron so that one side is opened at an edge thereof. Frame 21 formed along; A partition 22 partitioning the elongated hexahedron to a height at which the case 1 can be slid; And a handle 23 attached to the upper surface of the elongated hexahedron; the case 1 is pushed in from the open side of the frame 21, and the case 21 is divided into the frame 21 and the partition 22, respectively. 1) It is convenient because it can be stored and handled as a unit, and even if the case is stored in the rack 2, the frame 21 constituting the rack 2 has only a corner portion and the front, rear and side surfaces are opened. Since the upper and lower sides have partitions 22, it is difficult to contact the outside air. However, if the partition 22 is doubled and the partition 22 is also punched in the center, contact with the outside can be maximized. } There is no big problem in contact with the outside air, and there is no big difference with the temperature in the chamber 4. This is also due to the point of maximizing heat transfer to the hole 122 and the metal material, as described above.

And, the handle 23 is to be attached to the upper surface side, which is easy to lift while the rack (2) is located at the nearest distance to the inlet of the chamber (4) by storing the rack (2) in a vertical position usually. If the structure of the chamber 4 is changed or if the storage method of the rack 2 is adjusted, the positional shape of the handle 23 can be changed as much as possible.

In addition, by providing an accommodation portion that becomes the partition wall 31 on the base 3 on the plate, it is possible to maximize the utility of the present invention by convenient to build up a plurality of racks (2) in this storage portion, and this additional configuration Even if it does not deviate from the technical idea of the present invention, it is obvious that it is within the scope of the present invention.

According to another configuration of the present invention, in addition to the above-described configuration, the latch grooves 221 which are respectively drilled on the partition 31 and the opening of the upper surface of the rack 2, and the respective latch grooves 221, 221, Has a latch 24 penetrating through the same at the same time, which holds the rack 2 in which the plurality of cases 1 are housed, even if it is sideways during transport (open side down). ..., the clasp 24 penetrating through ... is provided as a stopper for preventing the case 1 from being undesirably released from the rack 2 by blocking the opening.

The present invention according to the above configuration is easy to handle a large amount of samples, such as blood bone marrow, and properly stored and stored, and further by specifying the sample to be stored by unit to prevent contamination or difficulty in handling between the samples, in particular It is an effective and useful design to provide a mechanism to prevent damage or contamination during transportation.

Claims (3)

To be stored in a chamber 4 of a liquid nitrogen tank in which a cryogenic material such as liquid nitrogen 41 is contained therein; A body 11 having a hinge hole 111 having a long hole at a side end, and a locking jaw 112 at an upper end thereof, and accommodating predetermined contents; And A guard 121 formed to guide the side portion of the body 11 to both sides, a hole 122 drilled in the center portion, and a hinge to which the head is caught in the hinge groove 111 of the body 11. A cover (12) having a cover (123) to cover the body (11); Cryogenic cryopreservation vessel sample holder of a biological material, characterized in that consisting of a case (1). The method of claim 1, Frame 21 is formed along the corner to open one side as a long cube; A partition 22 partitioning the elongated hexahedron to a height at which the case 1 can be slid; And Ultrasonic cryopreservation vessel sample holder for biological material, characterized in that it further comprises a rack (2) consisting of a handle (23) attached to the upper surface of the elongated hexahedron. The method according to claim 1 or 2, Has a latch groove 221 perforated on the partition 31 and the opening of the upper surface of the rack 2, and the latch 24 penetrates through the respective latch grooves 221, 221, ... at the same time. A sample holder for a cryogenic cryopreservation vessel of a biological material.