KR20020015540A - Food materials for preventing dementia and foods using the same - Google Patents

Abstract

The present invention is a medicinal plant of dementia, caustic, jinjin, eoseongcho, ogapi, astragalus, engineering, active in improving concentration and memory in patients with dementia, sulfur, yellow and white, Gaza, central nervous system Sewage, gold and silver, wood peel, kill, non-leaf, cheonggung, gardenia, and hot-water extracts or concentrates, such as circular wind, sand, lotus, cheonjak, bokbunja, which have been found to be anti-aging, have been identified. As a food material for the prevention of dementia-related diseases, it can be used in the manufacture of various foods.In particular, by adding them during the manufacturing process of Korean confectionery, dieting these confections can bring about prevention and improvement of various dementia-related diseases as well as aging in the future. The present invention relates to a method for producing a functional food for preventing dementia-related diseases that can reduce the frequency of dementia patients.

In addition, the present invention is a functional ingredient that promotes brain cell activity by using a hydrothermal concentrate obtained by adding a food material for preventing dementia-related diseases confirmed in the present invention in addition to the herbal medicines Baekboksin, Seokchangpo, Wonji used as a herbal medicine of Dongbobogam Also included is a method of making edible fruit for children.

Description

Food materials for preventing dementia and foods using the same {Food materials for preventing dementia and foods using the same}

The present invention relates to glycine binding site inhibition activity (Glycine binding site inhibition activity) involved in the regulation of motor and sensory function, Muscarine M1 receptor binding inhibition activity involved in concentration and memory, the central nervous system Food ingredients are prepared using various herbal extracts that exhibit acetylcholinesterase inhibition activity and antioxidant activity associated with anti-aging. The present invention relates to a method for producing functional confections that have the effect of preventing and ameliorating dementia-related diseases occurring in an aging society by adding them during the manufacturing process, and intelligent fruits that promote brain cell activity for children.

Dementia is a brain disorder that indicates a general cognitive impairment in a clear state of consciousness, usually caused by chronic or progressive brain disorders, and impaired in many senior cerebral functions such as memory, thinking ability, understanding, calculation, learning, and language judgment. to be. Dementia is the most common disease in which human deaths rank third in the world after coronary artery disease and cancer. Around 25 million people worldwide suffer from this disease.In 1960, out of a total of 25 million people, the elderly population was 720,000, 2.9%, but in 1990, about 2.12 million (5.0%), 1995 It is expected to become a full-fledged aging society as it has increased to about 2.5 million people (5.6%) and from 2000 to 7-8% of the total population. Therefore, the number of patients with dementia increases rapidly with age, with 6-8% of patients over 65 years old and 30% of patients over 85 years old.

Dementia-induced diseases include senile dementia, cerebrovascular dementia, Pick's disease, Creutzfeldt-jakob's disease, and Parkinson's disease. Dementia, dementia, hypothyroidism, normal pressure hydrocephalus, diabetes, drug addiction, anemia and vitamin deficiency. Geriatric dementia accounts for 50 to 60% of adult dementia in the West, with severe diffuse brain atrophy and loss of brain cells, resulting in loss of memory and intelligence, impaired abstract thinking and high cortical function. It is a symptom showing delusion and behavioral disorders, and it is a representative disease showing disorder of higher mental function and desolation of personality. In addition, cerebrovascular dementia caused by extensive brain lesions after stroke or by cerebral atherosclerosis is reported to be relatively higher in the East than in Europe.

In Oriental Medicine, it was the first to refer to the symptoms of dementia in terms of dementia. Since then, it has been referred to as Seokbinbinok, dialectic, and so on in terms of symptoms and treatment. The records were relatively detailed. Before that, dementia was identified by including other symptoms similar to dementia such as mania, forgetfulness, depression, phlegm, and erosion. I think. In terms of treatment, with the exception of some of the aforementioned treatable dementia, senile dementia, pick disease, and cerebrovascular dementia have not been identified. Therefore, dementia may be more effective than preventive treatment.

Many drugs have been studied for the treatment of dementia, but no drug has yet been proven effective. However, in addition to the hypothesis that cognitive dysfunction is mainly caused by damage to choline neurons at the base of the cerebral base, choline drugs with various mechanisms have been developed, and some have proven somewhat effective. There is a bar. Recently, donepezil, which has high receptor specificity, long half-life, and simple administration method, has been developed and used in Europe and the United States. In addition, possible methods for the next generation of therapies include a combination therapy of drugs acting on various neurotransmitters, nerve growth factors, and genetic engineering treatments. In other words, in addition to choline neurons, various neurotransmitter abnormalities and cerebral cortical atrophy have been reported, so the pathology of dementia is not limited to one neurotransmitter or part of the brain, but is specific to a wide range of brain regions and nerves. Combination therapy of choline / serotonin, choline / somatostatin-containing drugs with delivery agents is expected. Considering the low regenerative capacity of neurons, it has recently been recognized that clinical methods are needed to delay the degenerative process. One of these roles is the nerve growth factor, which has been shown to protect the degeneration of choline neurons that occur after surgery in animal experiments and to the growth, regeneration and maintenance of choline neurons. It is an essential protein. There is still a problem in the method to be administered directly to the brain, but if this problem is solved, it is expected to attract attention as a new treatment method. Recently, it has been confirmed that a gene causing dementia exists on chromosome 21, and studies to identify genes of beta-amyloid protein, one of the important neurological lesions, have been actively conducted to determine the possibility of gene therapy for the disease. I have suggested.

Therefore, the most reasonable new material for preventing and improving dementia should be a drug that delays the destruction and aging of brain cells, restores cognitive function, protects brain cells and promotes the regeneration of brain cells. There is no current. Therefore, it is necessary to appropriately select the drug according to the symptoms and causes of the patient, but the reality is that it is left to the doctor's skill. Currently used drugs include antipsychotics (Antipsychotics), antidepressants (Annticepressant), antianxiety (Antianxiety), anticonvulsant (Anticonvulsant), brain stimulants (Nootropics), enzyme blockers involved in the metabolism of neurotransmitters (Enzyme blocker), Calcium blockers (Ca-blocker), etc. are used, and the present comparatively definite therapeutic effects are acetylcholinesterase inhibitors among choline nervous system agents. Memory impairment is an early symptom of Alzheimer's disease and is a representative symptom. This memory impairment is known to be due to the decrease of acetylcholine, and after data collected through brain image study of dementia patients, severe degeneration of acetylcholine nervous system These are drugs that have been developed because of the therapeutic process that is focused on.

As described above, one of the rational methods for preventing and improving senile dementia-related diseases in the development of a medicinal treatment for dementia is preventing and improving food materials, concentration and memory disorders that prevent and improve motor, sensory, and mental disability. To develop a composition using a food material, and a food material that is active in preventing the degeneration of the central nervous system, and then added to the food to take effect by ingesting a certain amount, therefore the present invention is the above element, that is, exercise, The present invention relates to a method for producing traditional Korean foods, in which a new food material with active sensation, persistence, concentration, memory, and prevention of central nervous system is developed, the composition is prepared in combination with the material, and the overall activity of the above factors is exerted. .

The purpose of the present invention is to extract the hydrothermal extracts of herbal medicines that are active in the regulation of motor and sensory functions, herbal medicines that have been found to be active in concentration and memory loss, herbal medicines that are effective in preventing central nervous system degeneration, and antioxidative activity. To provide a food material used.

In addition, an object of the present invention is to prepare a composition in which the food material is mixed again to exhibit a comprehensive function on dementia-related diseases.

In addition, an object of the present invention is to provide a method for producing dementia preventive and improved functional Korean using a food material that is recognized for the dementia-related diseases.

And it is an object of the present invention to provide a food material and a method of manufacturing a children's intelligent fruit using the same food composition is added to the composition for promoting brain cell activity to the food material.

The present invention is effective in preventing the degeneration of cough, yellow white, Gaza, and central nervous system, which have been found to be active in the control of motor and sensory functions, such as aging, mountain medicine, jinjin, eoseongcho, ogapi, astragalus, and construction workers. Methods for manufacturing food materials using certified sewage, gold and silver, bark skin, killing, non-leafed leaves, celestial leaves, gardenia, and hot-water extracts such as wind, sand, yeonjak, sambaekcho, and bokbunja whose antioxidant activity has been identified, and the dementia-related diseases It relates to a method for producing a composition in which the food material is mixed again so as to exhibit a comprehensive function.

In another aspect, the present invention by adding the food material during the manufacturing process of the Chinese medicine using the herbal food material that is recognized as active in dementia-related diseases, functional herbal manufacture and various foods for dementia prevention and improvement exhibiting an effect on the prevention and improvement of dementia It relates to a method for producing a functional food exhibiting an effect on the prevention and improvement of dementia by adding the food material at the time of manufacture.

In addition, the present invention includes a food material prepared by adding the extract of herbal medicine Baekboksin, Seokchangpo, Wonji, which is used for Chongmyeongtang, which promotes memory and brain cell activity, to the food material, and a method for preparing a children's smart fruit using the same. do.

The herbal extracts of the present invention can be added to the food production or health food material or functional food material by a known method using a food hygiene method or pharmacologically acceptable prosthetics.

The herbal food material used in the present invention may be added in various formulations (extract, juice, hot water extract, powder, granules, grinding) according to the purpose.

The Chinese family referred to in the present invention includes all types of traditional family currently available on the market.

Food material for preventing dementia-related diseases of the present invention and a method of manufacturing a confectionery using the same Example 1 below: Method of manufacturing food materials for preventing and improving dementia, Example 2: Functional Chinese medicine manufacturing method for preventing and improving dementia Example 3: Illustrated by the intelligent and the manufacturing method for children, this embodiment should not be regarded as limiting the present invention.

Example 1. Method of manufacturing food materials for preventing and improving dementia

<The first process>

Medicinal materials of active and sensory functions such as aging, mountain chemicals, injin, eoseongcho, ogapi, astragalus, construction workers, concentration and memory active materials such as yellow lotus, yellow white, gaza, central nervous system active material sewage, gold silver flower, bark skin, killing, non-leaf leaf, 100g of the above materials of Cheongung, Gardenia, and anti-oxidative active materials, such as far wind, sand, lotus, baekseoncho, and bokbun, were thoroughly washed with water, and then 1500ml of water were extracted for 5 hours or longer at 95-100 ° C.

<The second process>

The hot water extract was filtered through a filter paper (whatman No. 3) to obtain 800 ml of pure herbal hot water extract, each of which was freed from debris.

<The third process>

Each filtered herbal extract is concentrated completely at 60 ° C. using a vacuum concentrator to obtain a powder.

<The fourth process>

The powder is diluted to a concentration of 0.005, 0.05, 0.5, 1.0 mg / ml with distilled water, and the activity of each herbal material mentioned in the first step is measured to determine whether dementia-related activity is detected. The results of various physiological activities for each herbal material show excellent dementia related activity as shown in Tables 1, 2 and 3.

<The fifth process>

Based on the results of the dementia-related physiological activity in the fourth step, aging, potions, injin, eoseongcho, ogapi, astragalus, 40g each of construction workers, 80g of rhubarb, 50g each of Gaza, sewage, gold silver flower, bark skin, kill, non-leaf leaves 10 liters of water was added to 910 g of the Chinese herbal medicine material, cheongung, gardenia gardenia, each 40g, and 50g each of the original wind, sagan, lotus root, three hundred seconds, and bokbunja, followed by hot water extraction at 95 ~ 100 ℃ for 5 hours. The concentrated solution 1200ml (40Brix) is obtained through the 3rd process.

<The sixth process>

The concentrated solution prepared in the fifth step is partially lyophilized and used for investigation of dementia-related activities, and the rest is used for the manufacture of functional Chinese medicine for preventing and improving dementia. The lyophilized product prepared as described above was found to have high related activity as shown in Table 4 when the dementia related activity was measured by diluting to 0.005, 0.05, 0.5, 1.0 mg / ml with distilled water.

Table 1. Glycine binding site inhibition activity for each herbal extract

Medicine Concentration (mg / ml) % Inhibition Heraldic 0.0050.050.51.0 5586100100 Acid pills 0.0050.050.51.0 5795100100 Jin 0.0050.050.51.0 4173100100 Air castle candles 0.0050.050.51.0 4071100100 Cuckold with feet 0.0050.050.51.0 4271100100 Yellow 0.0050.050.51.0 317894100 Public sign 0.0050.050.51.0 3473100100

Table 2. Muscarin M1 receptor binding inhibition activity for each herbal extract

Medicine Concentration (mg / ml) % Inhibition Yellow lotus 0.0050.050.51.0 4193100100 Sulfur bag 0.0050.050.51.0 01391100 Go 0.0050.050.51.0 03467100

Table 3. Acetylcholinesterase inhibition actibity for each herbal extract

Medicine Concentration (mg / ml) % Inhibition Sewage 0.0050.050.51.0 1352100100 Gold silver coins 0.0050.050.51.0 8528897 Throat 0.0050.050.51.0 01585100 Kill 0.0050.050.51.0 0147698 Non-cotyledon 0.0050.050.51.0 04279100 Celestial palace 0.0050.050.51.0 07087100 Gardenia 0.0050.050.51.0 034100100

Table 4. Dementia related activities of the herbal extract composition of the sixth step of Example 1

activation Concentration (mg / ml) % Inhibition Glycine binding inhibitory activity 0.0050.050.51.0 56100100100 Muscarinic M1 receptor binding inhibitory activity 0.0050.050.51.0 4887100100 Acetylcholinesterase Inhibitory Activity 0.0050.050.51.0 6491100100

The results of Table 1 and Table 2 study the effect of each drug on the glycine binding site inhibition (Mlycarine M1 receptor binding inhibition) activity and the relationship between the receptor-ligand In order to react with the receptor using a ligand attached to the radioisotope, the resultant is filtered through a glass fiber filter to remove the unbound extra ligand and then remains on the washed filter disc. The amount of the isotope was measured to quantify the binding reaction of the ligand to the receptor and to determine the effect of the drug.

Receptor sources for the present invention include human recombinant muscarin receptor subtypes 1 and 2 expressed in CHO cells to exclude interactions with various subtypes of each drug. M ₁ , M ₂ ) and glycine binding site (glycine binding site) receptor was used as a fraction of the receptor isolated from the rat brain. Cell fractions containing large amounts of these receptors were stored frozen and diluted to the optimal concentration.

Acetylcholinesterase inhibitory activity of Table 3 is obtained by reacting the product obtained by reacting thiocholine released from acetylcholine with DTNB (5,5'-dithio bis-2-nitrobenzoate). The activity was calculated by measuring.

In other words, glycine binding site inhibition and Muscarine M1 receptor binding inhibition activity were suspended in the buffer cell fractions stored at -70 ℃ in buffer for each experiment and the protein content was reduced. After confirming with the Bio-Rad DC Protein Assay Kit, it was adjusted to the optimal concentration for each receptor. At this time, the protein concentration represented by the content of the receptor is determined through the basic experiment. Samples of all tests were replicated and each buffer was used as the buffer required for the experiment. The final volume of the reaction was 0.25 ml, which contained 50 μl of hot-ligand and 10 μl of test drug. The reaction was started for 30-60 minutes at 27 ° C. by adding 100 μl of receptor suspension. In the first step drug search, the affinity of the drug for receptors was searched for two concentrations (1 μM and 10 μM). After incubation, the reaction was terminated by filtering with cold Tris buffer using an Inotech cell harvester system using a Wallac glass fiber filtermat GF / C. The isotope bound to the receptor was separated, washed, and the amount of isotope remaining in the filtermat was measured by a MicroBeta-Counter.

In the acetylcholinesterase inhibitory activity experiment, enzyme activity was produced by reacting thiocholine, which is released by hydrolysis of acetylcholine, which is a substrate, according to Elman's method, with DTNB. Enzymatic activity was measured by UV detection of dithio compounds and thio anions of nitrobenzoic acid. At this time, all reactions were fixed at 25 ° C. in a 1 ml cuvette to measure initial rates for 30 seconds and 60 seconds, and enzyme inhibition activity was calculated in terms of the initial rate ratio of the inhibition reaction to the reference reaction.

Example 2. A functional Korean confectionery for preventing and improving dementia

<The first process>

Selected glutinous rice (65%) is naturally fermented in water for about 2 weeks, washed well and ground to fine powder.

<The second process>

Jeongjong (1%), ginger juice (0.9%), refined salt (0.1%) is added to the glutinous rice kneaded well and steamed at 100 ℃.

<The third process>

Put steamed beans in the second process and add soy flour (19%) while striking to reduce the dough.

<The fourth process>

The raw material kneaded in the third step is put into a molding machine and then molded, and dried to 27% moisture at 27 ° C.

<The fifth process>

The dried raw material is fried in soybean oil (2%).

<The sixth process>

The confectionery fried in the fifth step is immersed in about 30 seconds to 1 minute in the syrup added to the herbal composition concentrated composition prepared in the fifth step of Example 1 at a concentration of 3 to 15%.

<The seventh process>

In the sixth step, coat the Korean confectionery coated with the herbal composition concentrated with white rice, and then dry it and package it.

Example 3 Smart Child Confectionery

<The first process>

Put 100g of sewage and 100g of sesame into 100g of Baeksin, 100g of Seokchangpo, 100g of raw paper, which is the herbal medicine of Donggibogam, and wash it well with water. Obtain 2600 ml of pure herbal hydrothermal extract.

<The second process>

The hydrothermal extract obtained in the first step is concentrated with a reduced pressure concentrator to obtain 680 ml (40 Brix) of concentrate.

<The third process>

Selected glutinous rice (65%) is naturally fermented in water for about 2 weeks, washed well and ground to fine powder.

<The fourth process>

Jeongjong (1%), ginger juice (0.9%), refined salt (0.1%) is added to the glutinous rice kneaded well and steamed at 100 ℃.

<The fifth process>

Add steamed steam from the 4th process to the lanyard and beat soybean paste (19%) and knead again.

<The sixth process>

The raw material kneaded in the fifth step is put into a molding machine and then molded, and dried to 27% moisture at 27 ° C.

<The seventh process>

The dried raw material is fried in soybean oil (2%).

<The eighth process>

The confections fried in step 7 are dipped in starch syrup added with 3 to 20% of the herbal composition prepared in step 1 for about 30 seconds to 1 minute.

<The ninth process>

In step 8, the Hanja coated with the herbal composition concentrate is dressed with white rice and rice, then dried naturally and packed.

The present invention is a medicinal plant of dementia, caustic, jinjin, eoseongcho, ogapi, astragalus, engineering, active in improving concentration and memory in patients with dementia, sulfur, yellow and white, Gaza, central nervous system Either alone or a mixture of hot water extracts or concentrates such as sewage, gold and silver, wood peel, dead, non-leafed leaves, celestial leaves, gardenia, and garden winds, soybeans, yeonjak, sambaekcho, and bokbunja, which have been certified to prevent degeneration, As a food material for the prevention of dementia-related diseases, it can be used in the manufacture of various foods, and in particular, by adding these foods in the manufacturing process of Korean confectionery, dieting these foods or confections can bring about prevention and improvement of various dementia-related diseases. Rather, it may reduce the incidence of dementia patients in an aging age.

In addition, the present invention is a functional food that promotes brain cell activity by using the hydrothermal concentrate obtained by the addition of sewage and acid powder confirmed in the present invention in addition to the herbal medicines Baekboksin, Seokchangpo, Wonji, which is the herbal medicine used in Dongbobogam. Also included is a method of making intelligent fruits.

Claims (9)

Extract water at 95-100 ° C. for at least 5 hours by adding water to a herbal medicine selected from the group consisting of lingering herb, medicinal herb, injin, eoseongcho, ogapi, astragalus and construction worker; An extract or a mixture of extracts prepared by concentrating the extract under reduced pressure; Food material to enhance the glycine binding inhibitory activity, characterized in that. Adding water to a herbal medicine selected from the group consisting of rhubarb, yellowish white and Gaza and extracting at 95-100 ° C. for at least 5 hours; An extract or a mixture of extracts prepared by concentrating the extract under reduced pressure; Food material to enhance the muscarinic M1 receptor binding inhibitory activity, characterized in that. Sewage, gold and silver, bark skin, kill, add water to the herbal medicine selected from the group consisting of non-leaf, cheongung, gardenia and extract at 95-100 ℃ for 5 hours or more; An extract or a mixture of extracts prepared by concentrating the extract under reduced pressure; Food material to enhance the acetylcholinesterase inhibitory activity, characterized in that. Extracting at 95-100 ° C. for at least 5 hours by adding water to a herbal medicine selected from the group consisting of worms, loaves, lotus roots, three hundred seconds, and bokbunja; An extract or a mixture of extracts prepared by concentrating the extract under reduced pressure; Food material to enhance the antioxidant activity, characterized in that. A functional food material for preventing and improving dementia, characterized in that at least two food materials selected from the group consisting of the food materials of claim 1 are mixed. In claim 5, Food for preventing and improving dementia, wherein the food material is added to Baek-sin and Seokchang-po and raw paper, followed by extracting at 95 to 100 ° C. for at least 5 hours, and further adding a concentrated food material obtained by cooling and filtering. Material. Functional food for the prevention and improvement of dementia, characterized in that using a food material selected from the group consisting of the food material of claim 1 to claim 4. Functional food for the prevention and improvement of dementia, characterized by using the food material of claim 5. Functional food for the prevention and improvement of dementia, characterized by using the food material of claim 6.