KR20010064389A - A cosmetic composition for the sensitive skin - Google Patents

Abstract

The present invention God (Magnolia flos) Extract, AstragalusAstragalus membranaceus) Extract, celestial (Cnidium officinale) Extract, rutin and gamma-orizanol as active ingredients, controlling the production of cytokines, hypersensitivity reactions of sensitive skin such as contact irritation or allergy It relates to a cosmetic composition for sensitive skin that can alleviate.

Description

A cosmetic composition for sensitive skin

The present invention relates to a cosmetic composition for sensitive skin containing sini extract, Astragalus extract, Cheongung extract, rutin, gamma-orizanol and the like.

It is well known that certain skin types are more sensitive than others, and skin scientists define sensitive skin as being more likely to exhibit contact irritation or allergic reactions than the average population.

In general, sensitive skin holders easily show hypersensitivity reactions such as erythema, skin drying, skin roughness, skin irritation, and allergic reactions to cosmetics and external influences such as stress and air pollution. The substance is said to be involved [J. Appl. Toxicol., Vol 16, p 65-70, 1996]. Therefore, the skin sensitization can be divided into two cases according to the pathogenesis of the inflammatory path of contact irritation and the case of allergic reaction. It is reported that erythema response is characteristic when following the inflammatory path of irritation, and interleukin-1 (IL-1) and tumor necrosis factor-α (TNF-α) are involved [M] . Lindberg, Contact Dermatitis, vol 35, p 355-360, 1996], in contrast, eczema is characteristic of following allergic reactions, and interleukin 6 (IL-6) and interleukin 8 (IL-8) are involved. Is being reported [MR Holiday, Am. J. Contact Dermat., Vol 8, 158-164, 1997; M. Lindberg Contact Dermatitis, vol 35, p 355-360, 1996].

In the past, in order to prevent the occurrence of such hypersensitivity reactions, only the components that may cause such hypersensitivity reactions were removed from the cosmetic components. However, due to environmental and psychological factors, it is also necessary for sensitive skin holders to use cosmetics that contain ingredients that are likely to cause the above irritation, rather than simply not using ingredients that may cause hypersensitivity reactions. It is necessary to alleviate hypersensitivity by controlling cytokines and inflammatory mediators, which are the root causes of hypersensitivity reactions. Furthermore, by controlling these cytokines or inflammatory mediators, it is possible to improve and normalize sensitive skin. Development of cosmetics is required, and such research is being actively conducted.

As a specific example, a cosmetic composition for sensitive skin containing a bradykinin antagonist (US Pat. No. 5,849,312), Tumor necrosis factor-α (TNF-α), which is one of cytokines Cosmetic compositions for sensitive skin containing inhibitors (US Pat. No. 5,851,556).

However, since they contain antagonists or cytokine inhibitors as active ingredients, they have many limitations in terms of safety and stability.

Therefore, the present inventors can improve the sensitive skin by alleviating the skin hypersensitivity reaction by controlling the inflammatory mediator or the cytokine which is the root cause of the skin hypersensitivity reaction, and can solve the safety problem in the cosmetic which can be normalized. As a result of searching for a new ingredient, the present inventors have found that god, which is already proved to be safe in cosmetics and medicines, has a very good cytokine regulating ability. .

Accordingly, an object of the present invention is to provide a safe and sensitive skin cosmetic composition that can alleviate skin hypersensitivity reactions such as inflammatory dermatitis and allergic reactions by skin irritation by controlling cytokines that cause skin hypersensitivity in sensitive skin. To provide.

In order to achieve the above object, the cosmetic composition for sensitive skin according to the present invention is one or more selected from the group consisting of shinyi extract, Astragalus extract, Cheongung extract, rutin (gamma) and gamma-orizanol (total composition) It is characterized by including in an amount of 0.001 to 10.0% by weight relative to.

Hereinafter, the present invention will be described in detail.

Magnolia flos has been used for headaches, toothaches and intestinal tracts in motion therapy, and has been used as a drug for sinusitis. In recent years, the anti-allergic and anti-inflammatory effects of the gods have been scientifically confirmed, demonstrating this empirical effect.

Astragalus membranaceus ( Astragalus membranaceus ) is a herbal medicine that has been recognized in China since ancient times, has an antioxidant and anti-inflammatory action, and is also used as a blood pressure lowering agent.

Cnidium officinale is said to have vasodilation and anti-inflammatory, antipyretic and analgesic treatments have been reported, and it is used for the treatment of blood, tonic, soothing and analgesic in oriental medicine.

Rutin is a flavonoid glycoside that is widely found in natural products. It is called vitamin P. It has the function of normalizing capillary permeability and thus protects against skin injuries caused by allergic reactions. It has been used in formulations for stimulating remission for strengthening (WO 98-02138). In addition, it has been used in cosmetics as an ultraviolet absorber, a whitening agent, a yellow pigment, etc. as an antioxidant function.

Gamma-oryzanol (γ-oyzanol) is a substance extracted from rice, which is found in rice bran oil (rice bran oil), has an antioxidant function similar to SOD (superoxide dismutase), and also lowers cholesterol in the blood (cholesterol).

The present invention has been found that the god, which has such efficacy, can regulate the extracts and rutin and gamma oryzanole cytokines of Astragalus and Cnidium, and the herbal extracts are usually obtained by a method used to extract an active ingredient from a plant. Can be. For example, water, methanol, ethanol, acetone, or a mixture of water or a mixture thereof is added to the herbal powders by about 2 to 20 times the amount of the herbal powders, and the temperature is reduced to 50 to 100 ° C in an extractor with a cooling capacitor. It can be heated for ˜24 hours to obtain an extract, which is then evaporated to give a dried product which can be blended into cosmetics. Rutin and gamma oryzanol can use commercially available products, but it is preferable to use a compound having a purity of 95% or more.

In order to obtain the desired effect in the present invention, the at least one component selected from the group consisting of the extract of the god Shin, Astragalus extract, Cheongung extract, rutin, gamma oryzanol is contained in an amount of 0.001 to 10.0% by weight relative to the total weight of the composition .

On the other hand, the cosmetic composition for sensitive skin according to the present invention, in addition to the above-mentioned active ingredient, functional ingredients such as retinol, AHA, sunscreen, and other preservatives, which exhibit various effects on the skin but cannot be used as a raw material for sensitive skin due to irritation. Can be used with

The cosmetics for sensitive skin of the present invention are not particularly limited in the formulation, but for example, supple cosmetics, astringent cosmetics, nourishing cosmetics, nutrition cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing It may have a formulation such as water, pack, powder, body lotion, body cream, body oil, body essence.

In addition, in the cosmetic composition for sensitive skin of each formulation, other ingredients other than the above-mentioned active ingredients can be appropriately selected and blended by those skilled in the art without difficulty according to the formulation or purpose of use of other cosmetics.

Hereinafter, the present invention will be described in detail with reference to Examples and Test Examples, but the following Examples and Test Examples are provided to explain the present invention in more detail, and are not intended to limit the present invention to the Examples or Test Examples.

<Reference Example>

Preparation of Herbal Extracts

After grinding 1 kg of each natural product (Shinyi, Astragalus, Cheongung), each 10 kg of 50% ethanol was added to the pulverized product, and heated at a temperature of 50 to 100 ° C. for 3 hours in an extractor equipped with a cooling capacitor. The filtrate filtered by Whatman No. 2 was concentrated under reduced pressure to obtain a natural product extract having a dry weight of 16.7 g, respectively.

Routine used the product of Sigma, and gamma-orizanol used the product of the hardening | curing property (TCI).

<Test Example 1>

Cytokine Regulation in Treating Contact Dermatitis Inflammatory Stimulator (SLS) in Animals

1) Stimulation source treatment

Sodium lauryl sulfate (SLS), an inflammatory stimulant of contact dermatitis, is well known as a stimulant. SLS alone or SLS and the sample of the reference example was treated with 100 μl at a concentration of 0.1% in the ears of the Balb / c mouse for a predetermined time (0, 6, 12 hours) (Table 1 1). At this time, the concentration of SLS was set at 0.1%, which was a positive reaction in the local lymph node assay (LLNA) test. After a predetermined time, the RNA was separated using a TRIZOL reagent and electrophoresed, so that only intact RNA was used for the RT-PCR test. RT-PCR was reverse transcribed using MuLV, a type of reverse transcriptase, and then subjected to PCR reaction (gene specific amplification reaction) for TNF-α and IL-1, respectively.

Cytokine regulation experiments in these animals are described in Clin. Exp. It carried out by the method described in Immunol., Vol 107, p 213-223.

3) results

When the SLS and the samples of Reference Examples 1 and 2 (non-treated group) are not blank and the SLS as the stimulus source is treated, and the SLS and the sample of the Reference Example are treated together, The degree of expression was compared. The expression rate was calculated by the following Equation 1.

In the above equation, the average value of the untreated group (blank) value when no test substance was included was 100. In this case, the error of IL-1 expression rate was ± 2.31, and the error of TNF-α expression rate was ± 3.56. . After PCR, each was subjected to agarose (agarose) -electrophoresis, which was then corrected by densitometry to the value of GAPDH (glyceraldehyde 3 phosphate dehydrogenase; triglyceride aldehyde dehydrogenase).

The results are shown in Table 1.

Cytokine Regulation in SLS Stimulator Treatment in Animals Test Quality IL-1 expression rate in stimulus treatment (%) TNF-α expression rate (%) during stimulus treatment SLS (0.1%) 169.4 ± 5.23 149.6 ± 4.23 SLS (0.1%) + Astragalus (0.5%) 118.7 ± 2.33 110.3 ± 3.78 SLS (0.1%) + Archery (0.5%) 115.8 ± 3.59 116.8 ± 5.23 SLS (0.1%) + God (0.5%) 125.6 ± 3.79 123.9 ± 4.21 SLS (0.1%) + routine (0.1%) 125.5 ± 7.65 120.3 ± 3.31 SLS (0.1%) + gamma-orizanol (0.1%) 138.1 ± 4.91 121.7 ± 5.61 * Expression of IL-1 in the blank (blank) 100 ± 2.31 * Expression of TNF-α in the blank (blank) 100 ± 3.56

As shown in Table 1, the expression of IL-1, TNF-a is increased by the stimulation of SLS, but it can be seen that their expression decreases as a result of co-treatment with Astragalus, Cheongung, Shin, Rutin or Gamma-orizanol. . In particular, it can be seen that Astragalus and Cervix inhibit the expression of TNF-a and IL-1 most effectively.

<Test Example 2>

Cytokine Regulation in Treating Allergens in Animals

It was performed in the same manner as in Test Example 1 except that PCR was performed on IL-6 and IL-8 using DNCB (dinitrochlorobenzene (Dinitro chlorobenzene)), which is a well-known allergen, as an irritant. In Test Example 2, when the average of the untreated group (blank) value was 100, the error of IL-6 expression rate was ± 2.9, and the error of IL-8 expression rate was ± 4.92.

The results are shown in Table 2.

Cytokine Regulation When Treating Allergens in Animals Test Quality IL-6 expression rate when allergens are treated IL-8 expression rate when treated with allergens (%) DNCB (0.01%) 178.5 ± 5.54 155.9 ± 4.52 DNCB (0.01%) + Astragalus (0.5%) 108.6 ± 3.21 113.9 ± 2.87 DNCB (0.01%) + Archery (0.5%) 113.8 ± 4.18 118.4 ± 2.48 DNCB (0.01%) + God (0.5%) 125.8 ± 2.29 119.7 ± 3.39 DNCB (0.01%) + routine (0.1%) 123.8 ± 4.51 120.8 ± 3.95 DNCB (0.01%) + Gamma Orizanol (0.1%) 120.9 ± 5.21 119.4 ± 8.92 * Expression of IL-6 in untreated group 100 ± 2.9 * Expression of IL-8 in untreated group 100 ± 4.92

As shown in Table 2, the expression of IL-6 and IL-8 is increased to 178% and 156% by DNCB treatment, respectively, but by the treatment of Astragalus extract, Cervix extract, Sinyi extract, Rutin, or gamma-orizanol It can be seen that their expression is inhibited. In particular, it can be seen that their expression is most effectively inhibited by the treatment of Astragalus.

<Test Example 3>

Stimulation relaxation test in human body

Thirty women in their twenties and thirties with sensitive skin with frequent skin troubles were prepared. A cosmetic composition containing Balsam of Peru as an irritant was made and irritated against human skin.

That is, by adding 0.1% by weight of Balsam Peru, which is a source of stimulation, to the compositions of Examples 1 to 25 and Comparative Example 1 of Table 4, after making and applying a patch for skin irritation test, the patch was applied after 4 days. The skin was removed and the skin reaction was visually examined and the test criteria were in accordance with the regulations of the International Contact Dermatitis Research Group (ICDRG).

On the other hand, Table 3 shows the composition ratio in the case of adding Balsam Peru as a stimulating source to the cosmetic composition in the form of nutrient cosmetics, Table 4 shows the composition ratio of the natural extract component shown as a test substance in Table 3 Example 1 It corresponds to -30 and the comparative example 1.

ingredient Composition ratio (%) Glyceryl stearate 1.50 Squalane 7.00 Mineral oil 7.00 Cetearyl Alcohol 1.20 Sorbitan stearate 0.30 Polysorbate 60 1.00 Carbomer 0.12 Glycerin 10.00 Test substance (natural extract component) 0 to 2.0 Irritation Source (Valsam Peru) 0.1 DI-water balance system 100.0

In the following Table 4, the case of not including any natural extract component was made as Comparative Example 1, and divided into Examples 1 to 30 according to the amount of the natural extract component. In this case, if the nutritional cosmetics formulation is to be implemented as a product, it should be made into a product except Valsamperu, which is an irritant.

Amount of test substance (natural extract component) in Table 3 (unit: weight%) Test substance Astragalus Extract Cheongung Extract Sinyi Extract Routine Gamma-Orizanol Example 1 0.5 - 0.5 - - Example 2 0.5 - - 0.1 - Example 3 0.5 - 0.5 - 0.1 Example 4 0.5 - - 0.1 0.1 Example 5 0.5 0.5 0.5 - - Example 6 0.5 0.5 - 0.1 = Example 7 0.5 0.5 0.5 - 0.1 Example 8 0.5 0.5 - 0.1 0.1 Example 9 0.5 0.5 0.5 0.1 - Example 10 0.5 0.5 0.5 0.1 0.1 Example 11 0.5 0.5 0.5 - 0.1 Example 12 - 0.5 0.5 - - Example 13 - 0.5 0.5 0.1 - Example 14 - 0.5 0.5 - 0.1 Example 15 - 0.5 0.5 0.1 0.1 Example 16 0.5 - 0.5 0.1 0.1 Example 17 0.5 - - - 0.1 Example 18 - 0.5 - 0.1 0.1 Example 19 - 0.5 - - 0.1 Example 20 - - 0.5 0.1 0.1 Example 21 - - 0.5 - 0.1 Example 22 - - - 0.1 0.1 Example 23 - - 0.5 0.1 0.1 Example 24 0.5 0.5 - 0.1 0.1 Example 25 - 0.5 0.5 - - Example 26 0.5 0.5 0.5 0.5 0.5 Example 27 0.8 0.8 0.8 0.8 0.8 Example 28 1.0 1.0 1.0 1.0 1.0 Example 29 1.5 1.5 1.5 1.5 1.5 Example 30 2.0 2.0 2.0 2.0 2.0 Comparative Example 1 - - - - -

The average value of the stimulus in this test was calculated according to the following equation (2).

In the following equation, the number of respondents is the number of people who showed stimulus response, the response intensity is the stimulus value determined according to the grade in the International Contact Dermatitis Research Group, the maximum response intensity is the highest value in the grade, and (4) is the highest value. Indicates.

As a result of the experiment, the average value of the stimulus and the decrease rate of stimulation are shown in Table 5. On the other hand, in Table 5, the stimulus reduction rate is based on Comparative Example 1, the degree of reduction in the average value of the stimulus than the value of Comparative Example 1 is expressed as a percentage.

Sample Number Mean of stimulus Stimulus reduction rate (%) Example 1 13.22 20.70 Example 2 14.98 10.14 Example 3 12.23 26.63 Example 4 15.65 6.12 Example 5 11.00 34.01 Example 6 9.26 44.45 Example 7 9.56 42.65 Example 8 9.43 43.43 Example 9 8.33 50.03 Example 10 6.68 60.05 Example 11 8.76 47.45 Example 12 11.28 32.33 Example 13 12.67 24.00 Example 14 11.99 28.07 Example 15 10.66 36.05 Example 16 13.48 19.14 Example 17 12.72 23.70 Example 18 13.21 20.76 Example 19 11.52 30.89 Example 20 11.18 32.93 Example 21 10.29 38.27 Example 22 11.31 32.15 Example 23 10.87 34.79 Example 24 9.21 44.75 Example 25 11.80 29.21 Example 26 8.2 50.8 Example 27 7.5 55.0 Example 28 7.0 58.1 Example 29 6.2 62.8 Example 30 5.6 66.4 Comparative Example 1 16.67 -

As shown in Table 5, in the case of Examples 10, 29 or 30, the stimulation caused by the stimulus material was reduced by 60% or more, which showed the most ideal efficacy. In addition, other embodiments are weaker than Example 10, but can see the stimulation relaxation function. They may be responsible for the normalization of sensitive skin by actually reducing the visible stimuli by the regulation of cytokines that cause sensitive skin.

Hereinafter, on the basis of the results of the above test example, by containing the extract from the god Shin, Astragalus extract, Cheongung extract, rutin, gamma oryzanol, by forming a cosmetic composition of various formulations that can alleviate the irritation of the sensitive skin and improve the skin present. However, the composition of the present invention is not limited only to the following examples, in particular, the extract of natural extracts, Astragalus extract, Cheongung extract, rutin, gamma oryzanol is easily carried out by those skilled in the art to adjust the various content ratio in the range of 0.001 to 10% can do.

<Formulation Example 1> Nutrition Cream

ingredient Composition ratio (% by weight) Beeswax 10.0 Polysorbate 60 1.5 Sebum 60 Cured Castor Oil 2.0 Sorbitan sesquioleate 0.5 Liquid paraffin 10.0 Squalane 5.0 Caprylic / Capricglycerides 5.0 glycerin 5.0 Butylene glycol 3.0 Astragalus Extract 2.0 Celestial extract 2.0 God extract 2.0 Routine 2.0 Gamma Orizanol 2.0 Purified water to 100

<Formulation Example 2> Massage Cream

ingredient Composition ratio (% by weight) Beeswax 10.0 Polysorbate 60 1.5 Liquid paraffin 40.0 Squalane 5.0 Caprylic / Capric Triglycerides 4.0 glycerin 5.0 Astragalus Extract 2.0 Celestial extract 2.0 God extract 2.0 Routine 2.0 Gamma Orizanol 2.0 Purified water to 100

<Formulation Example 3> Gel

ingredient Composition ratio (% by weight) Ethylenediamine Sodium Acetate 0.05% glycerin 5.0% Carboxy Vinyl Polymer 0.3% ethanol 5.0% Fiji 60 Cured Castor Oil 0.5% Triethanolamine 0.3% Astragalus Extract 2.0 Celestial extract 2.0 God extract 2.0 Routine 2.0 Gamma Orizanol 2.0 Purified water to 100

As described above, Astragalus extract, Cheongung extract, Sinyi extract, rutin and gamma-orizanol protect the skin from external stimuli and can be used as a substance for normalizing sensitive skin by regulating cytokines, a signaling substance for skin stimulation. Can be. Therefore, it can be used in conjunction with the functional raw material of concern other irritants, it can be used safely in cosmetics for sensitive skin.

Claims (1)

At least one selected from the group consisting of Magnolia flos extract, Astragalus membranaceus extract, Cnidium officinale extract, rutin and gamma-orizanol, based on the total weight of the composition, from 0.001 to 10.0. A cosmetic composition for sensitive skin, characterized in that it comprises in an amount of% by weight.