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KR20000061074A - Fungicide composition containing natural perfume materials - Google Patents

Fungicide composition containing natural perfume materials Download PDF

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KR20000061074A
KR20000061074A KR1019990009857A KR19990009857A KR20000061074A KR 20000061074 A KR20000061074 A KR 20000061074A KR 1019990009857 A KR1019990009857 A KR 1019990009857A KR 19990009857 A KR19990009857 A KR 19990009857A KR 20000061074 A KR20000061074 A KR 20000061074A
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oil
bactericidal
composition containing
fungicide composition
natural perfume
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Korean (ko)
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양용석
황경아
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성재갑
주식회사 엘지화학
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/10—Apiaceae or Umbelliferae [Carrot family], e.g. parsley, caraway, dill, lovage, fennel or snakebed
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/28—Myrtaceae [Myrtle family], e.g. teatree or clove
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40—Liliopsida [monocotyledons]
    • A01N65/44—Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N2300/00—Combinations or mixtures of active ingredients covered by classes A01N27/00 - A01N65/48 with other active or formulation relevant ingredients, e.g. specific carrier materials or surfactants, covered by classes A01N25/00 - A01N65/48

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Agronomy & Crop Science (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

본 발명은 천연 향료물질을 함유한 살균제조성물에 관한 것이다. 본 발명의 살균제조성물은 자동차 에어콘에서 서식하는 세균과 곰팡이에 대해 우수한 살균력을 갖는다.The present invention relates to a disinfectant composition containing a natural flavoring substance. The bactericidal composition of the present invention has excellent bactericidal power against bacteria and fungi inhabiting automobile air conditioners.

Description

천연 향료물질을 함유한 살균제 조성물{Fungicide composition containing natural perfume materials}Fungicide composition containing natural perfume materials

본 발명은 천연 향료물질을 함유한 살균제 조성물에 관한 것이다. 더욱 구체적으로 천연 향료물질로서 바질오일, 마조라오일, 레몬그라스오일, 클로버오일, 베이오일 또는 큐민오일을 함유시킴으로써 자동차 에어콘에서 서식하는 세균과 곰팡이에 대해 살균성능을 가진 살균제 조성물에 관한 것이다.The present invention relates to a fungicide composition containing a natural flavoring substance. More specifically, the present invention relates to a bactericide composition having bactericidal properties against bacteria and fungi inhabiting automobile air conditioners by containing basil oil, marjoram oil, lemongrass oil, clover oil, bay oil or cumin oil as natural flavoring substances.

종래의 살균효과가 있는 제품의 경우는 합성 살균제를 주원료로 하고 있어서 , 좁은 자동차내에서 사용할 경우 피부나 호흡기 등을 통해 인체에 흡수가 되고 개인에 따라 정도의 차이는 있지만, 두통, 알레르기, 피부 반점 등의 여러 가지 부작용을 나타내고 있다.In case of products with conventional sterilization effect, synthetic disinfectant is used as the main raw material. When used in a narrow car, it is absorbed by the human body through skin or respiratory system. Various side effects are shown.

본 발명자들은 합성 살균제의 사용에 따른 문제점을 개선하기 위해 연구실험한 결과, 천연 식물에서 추출한 향료 중 바질오일, 마조람오일, 레몬그라스오일, 클로버오일, 베이오일 또는 큐민오일을 선택 사용함으로써 특정 세균이나 곰팡이에 살균력이 우수한 살균제 조성물을 개발하게 되었다.The present inventors have conducted experiments to improve the problems caused by the use of synthetic fungicides. As a result, by selecting and using basil oil, marjoram oil, lemongrass oil, clover oil, bay oil or cumin oil among the flavors extracted from natural plants, To develop a fungicide composition with excellent bactericidal properties.

도면 제1도는 6종의 세균류 및 진균류에 대한 천연향료물질의 살균력(성장저지환)을 보여주는 사진이다.Figure 1 is a photograph showing the bactericidal power (growth-lowering ring) of the natural flavor material for six kinds of bacteria and fungi.

본 발명은 레몬그라스 오일, 큐민오일, 마조라오일, 바질오일, 클로버오일 및 베이오일 중에서 선택된 1 종 또는 그 이상의 천연향료물질을 함유하는 살균제 조성물에 관한 것이다.The present invention relates to a fungicide composition containing one or more natural perfume substances selected from lemongrass oil, cumin oil, marjoram oil, basil oil, clover oil and bay oil.

본 발명에서 천연 향료물질은 조성물의 총중량을 기준으로 10 내지 100 중량%를 함유할 수 있으나, 배합비율은 개인의 선호도에 따라 다양하게 변할 수 있다.Natural flavoring material in the present invention may contain 10 to 100% by weight based on the total weight of the composition, the blending ratio may vary in accordance with the preference of the individual.

자동차에어콘의 상재균 분리는 다음과 같은 방법으로 실시하였다. 차량용 에어콘을 가동시킨 후 에어샘플러에 한천띠를 삽입한다. 에어샘플러를 작동하여 일정시간 동안 에어콘 송풍구에서 나오는 공기를 포집한다. 한천띠를 빼내어 배양한다. 오염 미생물을 단일 콜로니로 분리하여 순수배양한다. 세균의 경우 비텍시스템을 이용하고 진균의 경우 현미경상에 나타나는 포자 및 균사의 형태를 관찰하여 동정한다. 그 결과 세균류로는 스타필로코쿠우스 아우리쿨라리스, 프세우도머나스 애루기노사 등이 주로 공통적으로 나왔고 진균류로는 페니실리움 종, 아스퍼길리우스 종 등이 주로 나왔다.The fungus isolate of the car air conditioner was carried out in the following manner. After starting the car air conditioner, insert the agar strip into the air sampler. Activate the air sampler to collect air from the air conditioner vent for a period of time. Remove the agar strip and incubate. Contaminating microorganisms are separated into single colonies and purely cultured. For bacteria, identify the spores and hyphae that appear on the microscope using the Vitek system. As a result, Staphylococcus auriculis and Pseudomernas aeruginosa were commonly used as bacteria, and Penicillium species and Aspergillus species were mainly used as fungi.

자동자 상재균중에서 세균류는 포도상구균 (Staphylococcus aureus)을, 진균류로는 흑곰팡이류 (Aspergillus sp.), 푸른곰팡이류 (Penicillium sp.)를 선택하여 실험하였고 또한 일반 주거환경에서 생성될 수 있는 대표적인 세균 중에서 대장균 (E.coli ATCC10536), 녹농균 (Pseudomonas aeruginosa), Aspergillus niger (흑곰팡이)를 대조군으로 실험하였다.Among the autologous flora, bacteria were tested by Staphylococcus aureus, fungi, black fungus (Aspergillus sp.), And fungi (Penicillium sp.). (E. coli ATCC10536), Pseudomonas aeruginosa, Aspergillus niger (black mold) were tested as a control.

시험 방법은 마루젤의 방법에 의한 페이퍼 디스크법을 사용하였다.The test method used the paper disc method by the method of Maruzel.

시험향료는 본발명의 향료물질로서 바질, 마조람, 레몬그라스, 클로버, 베이 및 큐민을 선택사용하고, 대조군으로서 무향, 타임, 퍼넬, 오크모스, 페루발삼, 그레이프후르트, 카모마일, 블랙페퍼, 벤조인 및 유칼립투스를 선택사용하였다. 먼저, 샤아레 (직경 80미리미터)에 세균 현탁액 또는 검은곰팡이의 포자현탁액을 1미리리터를 넣는다. 배지 10미리리터와 혼합하여 정치하여 배지를 만든다. 여기에 시험향료를 넣은 원형여지 (직경 6 미리미터, 두께 0.68 미리미터)를 배지 중앙에 놓는다. 곰팡이는 30 ℃에서만 48 시간, 세균은 37 ℃에서 24 시간을 배양한다. 각각의 향료에 대해서 곰팡이는 48 시간, 세균은 24 시간의 성장저지환의 직경을 측정한다. 위의 살균력 시험결과는 별첨한 도면에 나타내었다. 도면에서 성장저지환의 크기(mm)는 다음과 같이 평가한다.Test fragrances use basil, marjoram, lemongrass, clover, bay and cumin as the fragrance materials of the present invention, and as a control, fragrance free, thyme, funnel, oak moss, peru balsam, grapefruit, chamomile, black pepper, benzoin And eucalyptus were used. First, 1 milliliter of spore suspension of bacterial suspension or black mold is added to the saare (80 millimeters in diameter). Mix with 10 milliliters of medium and leave to form a medium. A circular filter (6 mm in diameter and 0.68 mm in thickness) with test perfume is placed in the center of the medium. Fungi are incubated for 48 hours only at 30 ° C and bacteria for 24 hours at 37 ° C. For each perfume, the diameter of growth-lowering ring was measured for 48 hours for mold and 24 hours for bacteria. The bactericidal test results are shown in the accompanying drawings. In the figure, the size of the growth-lowering ring (mm) is evaluated as follows.

(1) ＞6 : 성장저지대가 6센티미터이상이나 구분이 명확하지 않은 상태(1) ＞ 6: Growth zone is more than 6 centimeters but the division is not clear

(2) ＞8 : 샤아레의 크기가 8센티미터여서 더 이상의 측정이 불가능한 상태로 실제의 성장 저지대의 지름은 더 클 가능성이 많음.(2) ＞ 8: The size of the chairé is 8 centimeters, so that no further measurement is possible, and the diameter of the actual growth zone is likely to be larger.

(3) 사진 속의 성장 저지환의 크기와 밑의 수치에 약간의 차이가 있는 견본이 있으나 이는 성장 저지환의 크기 측정시기와 사진을 찍은 시기에 시간 차가 있었던 견본으로, 성장저지환의 크기의 수치가 기준이 되고 사진은 이후의 추이를 나타낸다.(3) There are samples with slight differences in the size of the growth inhibitory ring in the photograph and the values below, but this is a sample with a time difference between the measurement of the growth ring and the time when the photograph was taken. And the picture shows the subsequent trend.

위의 시험결과에 따라 균에 따른 각 천연향료의 살균력을 살펴보면 다음과 같다.According to the above test results, the bactericidal power of each natural flavor according to bacteria is as follows.

세균류:Bacteria:

대장균에서는 타임, 클로버, 베이 및 바질이 가장 우수했고, 마조람, 레몬그라스, 오크모스, 그레이프후르트 및 유칼립투스가 약간의 살균력을 보였고, 퍼넬 및 큐민은 미미한 살균력을 보였고, 카모마일, 블랙페퍼, 벤조인 및 페루발삼은 효과가 없었다.In Escherichia coli, thyme, clover, bay and basil were the best, marjoram, lemongrass, oak moss, grapefruit and eucalyptus showed slight bactericidal activity, funnel and cumin showed slight bactericidal activity, chamomile, black pepper, benzoin and Peruvian balsam did not work.

자동차에서 추출한 포도상구균에서는 타임 및 레몬그라스가 가장 우수했고, 바질, 베이 및 클로버가 중간 정도의 살균력을 보였고, 마조람 및 큐민이 미미한 살균력을 보였고, 블랙페퍼, 오크모스, 그레이프후르트, 카모마일 및 퍼넬이 약간의 살균력을 보였고, 유칼립투스, 벤조인 및 페루발삼은 효과가 없었다.Thyme and lemongrass were the best in car-derived staphylococci, with medium bactericidal activity with basil, bay and clover, with minimal disinfection with marjoram and cumin, black pepper, oak moss, grapefruit, chamomile and funnel. There was some bactericidal activity, and eucalyptus, benzoin and Peruvian balsam were ineffective.

녹농균에서는 모든 향료가 큰 효과를 보이지 못했는데 그중에서는 오크모스, 베이, 타임 및 클로버 정도가 약간의 살균력을 보였고, 유칼립투스, 바질, 마조람, 그레이프후르트, 블랙페퍼, 벤조인 및 큐민이 미미한 효과를 보였으며, 퍼넬, 카모마일, 레몬그라스 및 페루발삼은 살균력이 없었다.In Pseudomonas aeruginosa, all the fragrances did not show a significant effect, among which oak moss, bay, thyme and clover were slightly bactericidal, and eucalyptus, basil, marjoram, grapefruit, black pepper, benzoin and cumin had a slight effect. , Funnel, chamomile, lemongrass and Peruvian balsam were not bactericidal.

진균류:fungi:

흑곰팡이에서는 큐민, 레몬그라스, 타임 및 바질이 강력한 살균력을 나타내었고, 베이, 클로버, 퍼넬 및 오크모스가 높은 살균력을 보였고, 페루발삼, 벤조인 및 그레이프후루트가 약간의 살균력을 나타내었고, 유칼립투스, 마조람, 카모마일 및 블랙페퍼는 효과가 없었다.In black mold, cumin, lemongrass, thyme and basil showed strong bactericidal activity, bay, clover, funnel and oak moth showed high bactericidal activity, Peruvian balsam, benzoin and grapefruit showed some bactericidal activity, eucalyptus, Marjoram, chamomile and black pepper had no effect.

자동차에서 추출한 흑곰팡이류는 실험실에서 배양하는 종과는 약간 다른 종으로 각 천연향료의 살균력은 유사하였으나 바질의 살균력은 약간 저하하였고, 클로버 및 베이의 살균력은 약간 더 높은 경향을 보였다.Black molds extracted from automobiles were slightly different from those cultured in the laboratory. The bactericidal power of each natural flavor was similar, but the bactericidal power of basil was slightly decreased, and the bactericidal power of clover and bay was slightly higher.

자동차에서 추출한 푸른 곰팡이류는 바질, 레몬그라스 및 큐민이 강력한 살균력을 나타내었고, 타임이 이보다 약간 낮으나 강한 살균력을 보였고, 마조람 및 클로버가 우수한 살균력을 나타내었고, 페넬, 페루발삼, 오크모스, 벤조인 및 그레이프후르트가 우수한 살균력을 가지고 있었고, 유칼립투스, 카모마일 및 블랙페퍼는 효과가 약하거나 거의 없었다.Blue fungi extracted from automobiles showed strong sterilization of basil, lemongrass and cumin, slightly lower thyme but strong sterilization, and marjoram and clover with excellent sterilization, Fennel, Peruvian balsam, oak moss, benzoin and grapefruit. Fruit had good bactericidal power, and eucalyptus, chamomile and black pepper had little or no effect.

위의 시험결과로 볼 때, 본 발명에서 사용된 천연향료물질이 선택적인 살균력이 있음이 증명되었으며, 전체적인 살균력이 강한 향과 선택적으로 살균력이 강한 향을 실험을 통해 찾을 수 있었다.From the above test results, it was proved that the natural flavoring material used in the present invention has a selective bactericidal power, the overall sterilizing power and the selective sterilizing power was found through experiments.

위와같은 시험결과를 바탕으로 하기 표 1에 나타낸 천연향료 및 조성비율을 사용하여 살균제 조성물을 제조하였다.Based on the above test results, a fungicide composition was prepared using the natural flavor and composition ratio shown in Table 1 below.

성분ingredient 실시예 1Example 1 실시예 2Example 2 실시예 3Example 3 비교예 1Comparative Example 1 비교예 2Comparative Example 2 바질basil 10 %10% 20 %20% 30 %30% -- -- 마조람Marjoram 10 %10% -- 10 %10% -- -- 레몬그라스Lemongrass 50 %50% -- 30 %30% -- -- 클로버clover 10 %10% -- 10 %10% -- -- 베이Bay 20%20% 20%20% 20 %20% -- -- 큐민Cumin -- 60 %60% -- -- -- 타임time -- -- -- 50 %50% -- 퍼넬Funnel -- -- -- -- 10 %10% 오크모스Oak moss -- -- -- -- 10 %10% 페루발삼Peru Balsam -- -- -- 10 %10% 10 %10% 그레이프후르트Grapefruit -- -- -- 10 %10% 10 %10% 카바모일Cabamo -- -- -- 10 %10% 10 %10% 블랙페퍼Black Pepper -- -- -- 10 %10% 10 %10% 벤조인Benzoin -- -- -- 10 %10% 20 %20% 오크모스Oak moss -- -- -- -- 20 %20%

앞서 기술한 시험 방법과 동일하게 실시예 1, 2, 3 및 비교예 1, 2 의 살균제조성물을 시험하였다. 그 시험결과는 하기 표2에 나타내었다.The bactericidal compositions of Examples 1, 2 and 3 and Comparative Examples 1 and 2 were tested in the same manner as the test methods described above. The test results are shown in Table 2 below.

대장균(E.coliATCC10536)Escherichia coli (E. coliATCC10536) 포도상구균(Staphylococcusaureus)Staphylococcusaureus 녹농균(Pseudomonasaeruginosa)Pseudomonas aeruginosa 흑곰팡이(Aspergillusniger)Black fungus (Aspergillusniger) 흑곰팡이류(Aspergillus sp.)Black fungus (Aspergillus sp.) 푸른곰팡이류(Penicillium sp.)Blue mold (Penicillium sp.) 실시예 1Example 1 1.51.5 2.82.8 1.01.0 ＞8** ＞ 8 ** ＞8** ＞ 8 ** ＞8** ＞ 8 ** 실시예 2Example 2 1.51.5 2.52.5 0.80.8 ＞8** ＞ 8 ** ＞8** ＞ 8 ** ＞8** ＞ 8 ** 실시예 3Example 3 1.41.4 2.62.6 1.01.0 77 77 77 비교예 1Comparative Example 1 1.51.5 2.72.7 0.80.8 66 66 ＞6* ＞ 6 * 비교예 2Comparative Example 2 1.11.1 0.80.8 -- 0.80.8 0.80.8 1.61.6

* ＞6 : 성장저지대가 6센티미터이상이나 구분이 명확하지 않은 상태* ＞ 6: Growth base is more than 6 centimeters but the division is not clear

** ＞8 : 샤아레의 크기가 8센티미터여서 더 이상의 측정이 불가능한 상태로 실제의 성장 저지대의 지름은 더 클 가능성이 많음.** ＞ 8: The size of the chairé is 8 centimeters, so no further measurements are possible.

상기의 실험결과 본 발명에서 적용한 향료 살균성분은 기존의 문헌에 살균력이 가장 우수하다고 알려진 타임에 비해서 거의 대등하거나 우수한 살균력을 나타내었다.As a result of the above experiment, the fragrance sterilizing component applied in the present invention showed almost equivalent or superior sterilizing power compared to the time known to be excellent in the conventional literature.

본 발명의 살균제조성물은 자동차 에어콘에서 서식하는 세균과 곰팡이에 대해 우수한 살균력을 가지며 또한 합성살균제를 사용하지 않으므로 인체에 부작용이 없다.The bactericidal composition of the present invention has excellent bactericidal power against bacteria and fungi inhabiting automobile air conditioners, and also does not use synthetic bactericides, and thus has no side effects on the human body.

Claims (2)

레몬그라스 오일, 큐민오일, 마조라오일, 바질오일, 클로버오일 및 베이오일 중에서 선택된 1 종 또는 그 이상의 천연향료물질을 함유하는 살균제 조성물.A fungicide composition containing one or more natural perfume substances selected from lemongrass oil, cumin oil, marjoram oil, basil oil, clover oil and bay oil. 제1 항에 있어서, 천연향료물질을 10 내지 100 중량% 함유하는 살균제 조성물.The fungicide composition according to claim 1, which contains 10 to 100% by weight of natural perfume substance.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20000024484A (en) * 2000-02-16 2000-05-06 성재갑 Antifungal composition and method to be fumigated with heating

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20000024484A (en) * 2000-02-16 2000-05-06 성재갑 Antifungal composition and method to be fumigated with heating

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