KR19990081362A - Medicinal soap composition containing natural fig isolate - Google Patents

Abstract

The present invention is characterized in that the alkali metal soap base contains 0.05 to 25% by weight of the solvent extract isolate of fig fruit or leaves of Masui Dauphine, Bonsi or Whitezeano species, and 0.1 to 5.0% by weight of stabilizer. It relates to a medicinal soap composition. Medicinal soap composition of the present invention is excellent in the therapeutic effect of skin athlete's foot and fungus and antibacterial without toxic or side effects.

Description

Medicinal soap composition containing natural fig isolate

The present invention relates to a medicinal soap composition having antifungal and antifungal properties containing a natural fig separation component.

Fig is a long-established traditional food that has no pesticides at all and is a completely pollution-free fruit. It is closely related to the prevention of adult diseases. It has excellent food value and contains various antimicrobial active substances such as ficine and minerals. This is known to exist a lot. Figs are also used as athlete's foot and insect repellent, and there are many active substances in fig leaves. However, no technique has been attempted to manufacture soap preparations by extracting fig leaves or berries and separating pharmacologically active substances to measure antimicrobial activity of each active substance and using natural antimicrobial substances directly in the soap base. none.

In particular, chemosynthetic therapies account for a large number of antifungal agents. Among them, ketoconazole and itraconazole, developed by Texen, are widely used for antifungal agents such as ringworm (athlete), seborrheic dermatitis, sensitization, and sensitization caused by Candina and dermatophytes. It is also known to be effective against cardiac fungi and superficial fungi (see US Pat. No. 4,335,125). In addition, pharmaceutical compositions suitable for the treatment of fungal skin diseases by topical administration, in particular, compositions of solutions such as solutions, pastes, ointments, and cosmetics, that is, skin milk croshon, etc. are well known as microscopic application pharmaceutical compositions (Korea Patent Publication No. 90-17590). However, these chemical synthetic agents are difficult to distribute and accumulate in a sufficient amount of drugs because fungal hepatitis occurs in nails and toenails, skin or hair with little or no blood vessel distribution, and in case of heart disease, it is difficult to penetrate deeply. In addition, such synthetic materials cause side effects such as hepatotoxicity and gastrointestinal disorders when orally administered ketoconazole over a long period of time, and many antifungal agents are generally nonpolar and are insoluble in polar water or polar solvents.

On the other hand, as a topical pharmaceutical composition, these pharmaceutical compositions require a large amount of drugs when the outside of the skin is large, and depending on the amount applied, it is difficult to apply topically to a ringworm such as skin disease, causing foreign body feelings and discomfort. In the case of semi-solid high viscosity (for example, ointment, cream) after application, there is a problem that the pharmaceutical composition remaining on the skin clings to the coating, causing discomfort.

In addition, the existing alkali metal soap has a washing action against various foreign substances and bacteria, but the therapeutic effect against various skin diseases due to fungi and antibacterial can hardly be expected. In addition, preparations using fungicides such as ketoconazole and itraconazole, which are known as chemotherapy agents, have been developed, but these may also have side effects on the skin when used for a long time, and are particularly poorly soluble in polar solubility, especially in shampoos and liquid soaps. There may be unpleasant feelings such as foreign substance appearance, and it may not be able to exert its effects sufficiently due to the non-uniform shape. If these formulations are applied to soaps, the compounds are extremely poorly soluble in water, and their efficacy cannot be exceeded due to the aggregation.

Accordingly, the present inventors have made a thorough study to solve the problems of the conventional topical pharmaceutical composition described above, and obtained by extracting and separating strong antifungal and antibacterial ingredients from fig fruits or leaves that can be eaten as natural fruits in conventional alkali metal soaps. The present invention has been completed by discovering that fractional substances can be used to treat skin athlete's foot fungus and fungi and antibacterial effects without toxicity or side effects.

Figure 1 shows the extraction and separation process of the fig fruit.

2 shows the extraction and separation of fig leaves.

The present invention is characterized in that the alkali metal soap base contains 0.05 to 25% by weight of the solvent extract isolate of fig fruit or leaves of Masui Dauphine, Bonsi or Whitezeano species, and 0.1 to 5.0% by weight of stabilizer. It relates to a medicinal soap composition.

The present invention is to prepare a medicated soap by applying the compounds extracted and separated from the fig leaf or fruit using a solvent to the alkali metal soap base. Fig leaf and fruit used in the present invention is the sampling time and the extraction concentration is important. It is preferable to use fruits and leaves of all seasons with figs, and more particularly, to use fig fruits and fig leaves which are all ripe fruits and immature fruits such as June and October. It is preferably concentrated under reduced pressure at a low temperature of 20-70 ℃ range.

Specifically, the fig fruit or leaf which is a natural substance is extracted with methanol, which is an organic solvent, by dipping, pressing, or grinding, to obtain a mixed extract, which is an organic solvent, ethyl acetate, n-butanol, ethyl ether, nucleic acid, Extraction is carried out with methylene chloride or water, respectively, to obtain a fraction extract, which is also carried out in a continuous manner in an acidic solvent having a pH 2-6.5 or an alkaline solvent having a pH of 7.5-10 with saturated butanol at a temperature of 30-90 캜, more preferably. Fractional extraction is performed depending on the type of solvent for 2 to 36 hours at a temperature of 50-70 ℃ to selectively remove proteins and sugars to increase the efficiency of the amount of the composition. In addition, decolorization was carried out by carbon treatment and the solution was neutralized with alkali and acid, and the separated fraction active material was obtained through organic solvents, ethyl acetate, n-butanol, ethyl ether, nucleic acid, chloroform, methylene chloride and water, which were then subjected to column chromatography. Silica gel (SiO ₂ ), alumina oxide (Al ₂ O ₃ ) and developing solvent (chloroform: butanol = 70: 30, methanol, ethyl acetate: chloroform: = 70: 30, n-butanol: chloroform: nucleic acid = 30:60:10, chloroform: methanol = 70: 30, n-butanol: distilled water: chloroform = 10: 5: 80 and chloroform: methanol = 50: 50) And compounds A, B, C, D, E, F, G, a, b, c, d, e, f, g described in FIG. 2 are used as the main raw materials.

According to the present invention, the fig fruit or leaf is extracted by dipping, pressing or pulverizing with an organic solvent, methanol, to obtain a mixed extract, to obtain a fraction extract using each organic solvent, and to obtain a column extract in a continuous method. Using chromatography, the organic solvents, ethyl acetate, n-butanol, ethyl ether, nucleic acid, chloroform, methylene chloride, and water, are separated from each other using the mixed solvent and a single solvent. To prepare a medicated soap composition.

In the medicinal soap composition of the present invention, the organic solvent extract isolate of the fig fruit or leaf is preferably added in an amount of 0.05 to 25 parts by weight, preferably 0.1 to 12 parts by weight, to the alkali metal soap. When the fig separation component is added at 0.05 parts by weight or less, the effect of treating skin diseases is insignificant, and when it is used at 25 parts by weight or more, it is difficult to form a soap formulation and no antifungal soap effect can be expected.

Alkali metal soap in the medicated soap composition according to the present invention can be mixed up to 40 parts by weight by mixing the calcium salt fatty acid contained in the fig itself. Alkali metal soaps are alkali metal salts of fatty acids having 10 to 25 carbon atoms, from which fatty acid palm oil, fig oil, castor oil, olive oil, palm oil, soybean oil, palm kernel oil and other vegetable oils and fats, oil, pig oil, fish oil, etc. Get Alkali metals neutralize fatty acids either alone or in admixture with sodium or potassium to produce alkali metal soaps.

The medicated soap composition of the present invention may use a stabilizer for the stabilization of the anti-fungal and antimicrobial fig separation component of the active ingredient. Stabilizers include vitamin C, citric acid, sodium citrate, malic acid, sodium carbonate, sodium bicarbonate, sodium thiosulfate, sodium bisulfite, thiourea, tocopherol, tocopherol acetate, sodium ascorbate, anhydrous sodium bisulfite, sodium sulfite, imideurea, Selected from glutathione, polyphenol, butylated hydroxy anisole, butylated hydroxy toluene, ascorbyl palmitate, ascorbyl oleate, polysorbate 80, oleic acid, D.B.A (alkylbenzenesulfonic acid) Use 1 type, or 2 or more types.

The pharmaceutical soap composition of the present invention preferably contains 0.1 to 5.0 parts by weight, preferably 0.1 to 2.0 parts by weight. When the stabilizer is used in an amount of 0.1 parts by weight or less, sufficient stabilization effect cannot be expected, and when used in an amount of 5.0 parts by weight or more, side effects such as skin irritation occur and the stabilization effect is small.

The medicated soap composition of the present invention may also contain carrier components commonly used in cosmetic soaps. For example, a metal ion sequestrant may be added at about 0.2 parts by weight or less, and flavors, dyes, pigments, and the like may be added at a level of about 6.0 parts by weight or less. The medicated soap composition according to the present invention can be prepared according to a general soap manufacturing method. For example, put a makeup soap base containing 8 to 25 parts by weight of water in a constant mixer, add figs containing antifungal and antibacterial ingredients as active ingredients, add fragrances, dyes and other additives, and then stir with a stirrer. The agent may be prepared by mixing one kind or two or more kinds and then extruding, molding and cutting.

Soap base and sodium salt and potassium salt of palm oil (palm oil) which is a commonly used natural fat is used, preferably palm fatty acid sodium and potassium salts having 10 to 25 carbon atoms are used. In the medicated soap composition according to the present invention, about 50 to 98 parts by weight of a soap base (soap base) containing 8.0 to 25.0 parts by weight of water is used.

The medicated soap composition according to the present invention may contain 0.1 to 5 parts by weight of a moisturizing agent such as jojoba oil, glycerin polyethylene glycol (molecular weight 4000), ethylene glycol.

The medicated soap composition according to the present invention is applied to skin diseases caused by fungi three times a day, two days three days, and two or three times three to four days. It has an excellent therapeutic effect on most skin diseases caused.

The medicated soap composition according to the present invention can be used every day as a general cosmetic soap, and in particular, it can simultaneously combine the cleaning effect and the treatment of skin bacteremia and skin fungus, and is easy to use continuously in the affected area with very low one-time use. It is a new form of dandruff and scalp and skin fungal and bacterial treatments that has significantly improved the therapeutic effect and usability.

Medicinal soap composition according to the present invention is a shampoo, liquid soap, rinse, antimicrobial agent, fish disease treatment agent, fish feed additive, food processing additives, pollution-free plant during planting, pesticide substitute, athlete's foot treatment, skin ointment or skin disease treatment It can be usefully used.

Hereinafter, although this invention is demonstrated concretely based on an Example, the technical scope of this invention is not restrict | limited to these Examples.

Example

1) Experimental sample

Fig (Ficus carica Linn) is a cultivar produced in the area near Sanho-myeon, Samho-myeon, Yeongam-gun, Jeollanam-do (Ficus carica Linn.var.hortensis Shinn) and the leaves or berries of Masui Dauphine, Bongsi, and White Genoa species. Each was collected and tested.

2) Fig extraction method and solution fraction using solvent

Fig fig raw fruits and wet leaves are separately or ground based on 10 kg each, and fig raw fruits and fig leaves are extracted by dipping, pressing or grinding for about 3 months at room temperature by adding 10 times the amount of each solvent with methanol or ethanol. The resultant was filtered by suction with a Bchner funnel, and the extracts were mixed and the methanol was removed using a reduced pressure concentrator (Bchi RE 121, Switzerland) at 25 to 50 ° C., which was used as a sample and stored in a refrigerator compartment.

Solvent fractionation was performed based on the methanol extract (fresh fruit) 132g by the above method. That is, extract extract fractions using CHCl ₃ , CH ₂ Cl ₂ , EtOAc, CH ₃ CN, ether three times 1 L each extract and concentrated, and then the aqueous layer was solvent fractionated with saturated butanol and then concentrated 1.2 g of each fraction was obtained, and finally 28.4 g of fractions were obtained as samples and separated by column chromatography. The solvent was fractionated as shown in FIG. 2 based on 132 g of methanol (leaf). That is, the extract extract fractions were extracted and concentrated three times by 1 L each time selectively using MeOH, n-BuOH, CH ₃ CN, CHCl ₃ , EtOAc, CH ₂ Cl ₂ and then the aqueous layer was solvent-fractionated with saturated butanol. And then concentrated to give 5.6 g of fractions which were then separated using column chromatography.

3) Reversed phase chromatography (C18 Lobacolumn) and normal phase chromatography (silicagel 60) were used for column chromatography for separation of pharmacologically active ingredients. 1 shows the extraction and separation process of fig fruit, and FIG. 2 shows the extraction and separation process of fig leaf.

Example 1: Extraction process of [A], [a]

After washing the figs cleanly, remove the water, extract the fig raw fruits and the fig leaves with methanol by adding 1.5-10 times the amount of each solvent, based on 10 kg of the fig raw fruits and the wet leaves. Suction filtration with a Bchner funnel extracts and releases enough fig juice. In order to increase the extraction efficiency was extracted up to about 20-70 days. The extract was separated from the figs and then methanol was removed using a vacuum concentrator (Bchi RE 121, Switzerland) at 30-60 ° C. (fruit-323.5 g, leaf-267.0 g) and dark brown viscous compound in liquid syrup [A] , [a] were obtained, respectively, and used as samples as being stored in the refrigerator compartment.

Example 2: Extraction process of [A], [a]

After washing the figs, the water is removed, and the fig raw fruits and fig leaves are submerged at room temperature for about 2 months by adding 1.5-10 times the amount of each solvent with n-butanol at room temperature based on 10 kg of fig raw fruits and wet leaves. Extraction by means of the method and suction filtration with Bchner funnel sufficiently extracts and releases the fig juice. In order to increase the extraction efficiency was extracted up to about 20-70 days. The extract is separated from the figs, and 400 ml of distilled water is added and extracted at room temperature for about 3 hours. After filtering, butanol was removed using a vacuum concentrator (Bchi RE 121, Switzerland) (fruit-220g, leaf-210g) to obtain a dark brown viscous compound [A] and [a] in liquid syrup. Each was used while storing in a refrigerator.

Example 3: Extraction process of [A], [a]

After washing the figs cleanly, remove the water, and apply the fresh raw figs and fig leaves 1.5-10 times of each solvent with methanol based on 10 kg of fig raw fruits and wet leaves separately, and force them for 6 hours at 40-50 ℃. Extraction and suction filtration with Bchner funnel extract and release the fig juice. In order to increase the extraction efficiency, it is preferable to extract about 10 hours or more. The mixture was cooled, the extract was separated from the fig, and the methanol was removed using a vacuum concentrator (Bchi RE 121, Switzerland) at 30-60 ° C. (fruit-351 g, leaf-310 g). A dark brown viscous compound in liquid syrup [A ], [a] were obtained and used as samples as the samples were stored in the refrigerator compartment.

Example 4: Extraction process of [A], [a]

After washing the figs cleanly, remove the water, add 1.5-2 times the amount of figs with normal butanol, and extract the fresh fruits with normal butanol at room temperature, compress them with solvent, suction filtration with Bchner funnel, and extract juice. After separating from the fig and using a depressurizer (Bchi RE 121, Switzerland) at 30-60 ℃ to remove the normal butanol, 312g of a liquid syrup-like dark brown viscous compound [A], [a] was obtained. Each was used while storing in the refrigerator compartment.

Example 5: Extraction process of [A]

After washing the figs cleanly, remove the water, add 1.5-2 times the amount of each fig of the fig raw fruit with ethanol, and juice it with the solvent by annealing at room temperature, suction filtration with Bchner funnel, and extract the juice. After separation with an atomized solution, normal butanol was removed using a vacuum concentrator (Bchi RE 121, Switzerland) at 30-60 ° C., and 312 g of a dark brown viscous compound [A] was obtained as a sample. Used while storing in.

Example 6: Extraction process of [A]

After washing the figs cleanly, remove water and add 1.5-2 times the amount of each fig fruit to ethanol based on the fig raw and 10 kg, extract organic materials at room temperature for 20 days or more by dipping at room temperature, and use Bchner funnel. The resultant was filtered by suction, the juice was separated from the fig, and the ethanol was removed using a vacuum concentrator (Bchi RE 121, Switzerland) at 30-40 ° C., whereby 325 g of a dark brown viscous compound [A] was obtained. Each sample was used while storing in a refrigerator compartment.

Example 7: Extraction process of [B]

The solvent was fractionated as shown in FIG. 1 based on 132 g of the methanol extract (fresh fruit). That is, extract 3 times 1 L each of CHCl ₃ from the fig extract fraction, decolorized using 2.2 g of activated charcoal, and filtered. The filtrate was concentrated and column chromatography (developing solvent n-butanol: distilled water: chloroform = 10: 5: 80 and Chloroform: methanol = 50:50) was carried out to obtain 0.15 g of solid compound [B].

TLC data (developing solvent: chloroform: butanol: n-hexane = 85%: 10%: 5%): Rf = 0.89

IR spectral data IR (KBr), Cm-1: 1724 (CO), 1653, 1450 (C = C)

¹ H-NMR spectral data δ: 6.5 (1H, d, J = 9.6 Hz, H-3), 6.9 (1H, d, J = 3.2 Hz, H-3 '), 7.5 (1H, s, H -8), 7.8 (6H, s, H-5), 7.6 (1H, d, J = 1.7 Hz, H-2 ') 7.8 (H, d, J = 1.7 Hz, H-4)

Mass spectra showed ion peaks at m / z186, base peaks at m / z 131, and fragment ion peaks at m / z168.9, 158,147, and 118.9.

Example 8: Extraction process of [C]

Methanol extract (fresh fruit) based on 132g solvent fractionation as shown in Figure 1. That is, extract 3 times 1 L each of CHCl ₃ from the fig extract fraction, and then decolorized and filtered using 2.1 g, and the filtrate was concentrated and column chromatography (developing solvent normal butanol: distilled water: chloroform = 10: 5: 80 and chloroform: Methanol = 50:50) to obtain 0.21 g of compound [C].

TLC data (developing solvent: chloroform: butanol: n-hexane = 85%: 10%: 5%): Rf = 0.44

IR spectral data (KBr), Cm-1: 3013, 1679, 1416, 1324, 1200

Example 9: Extraction process of [D]

Solvent fractionation based on the methanol extract (fresh fruit) 132g by the method according to Example 1 as shown in FIG. That is, the extract was extracted with filtrate in 400 ml of chloroform and extracted and concentrated under reduced pressure to obtain 12 g. The mixture was added to 300 ml of ether again, and extracted with 1.2 g of activated carbon. The mixture was stirred at 40 ° C. for about 10 minutes and filtered. The filtrate was concentrated under reduced pressure, and extracted again with 300 ml of acetonitrile. Then, 1.2 g of the activated carbon was dissolved in the dissolved compound at 40 ° C. for about 10 minutes and filtered. The filtrate was concentrated and dissolved to perform column chromatography (developing solvent normal butanol: distilled water: chloroform = 10: 5: 80 and chloroform: methanol = 50:50) to obtain 0.28 g of crystalline compound [D].

TLC (developing solvent: coloform: butanol: n-hexane = 85%: 10%: 5%): Rf = 0.68

IR Spectrum Data IR (KBr), Cm-1: 3500 (OH), 2983 (CH), 1650, 1400 (C = C)

¹ H-NMR spectral data δ: 1.0 (mH, m), 3.3 (1H, m, H-3), 5.3 (1H, m, H-6)

Mass spectra showed ion peaks at m / z 414 and fragment ion peaks at m / z 303, 254, 213, 159, 146, 107 and 95.

Example 10 Extraction of [E]

Solvent fractionation based on the methanol extract (fresh fruit) 132g by the method according to Example 1 as shown in FIG. That is, the extract was put into 400 ml of dichloromethane and extracted from the filtrate fraction. The insoluble compound was added to 300 ml of ethyl acetate, followed by extraction. The layers were separated, dried over 0.12 g of magnesium sulfate, and concentrated under reduced pressure to obtain 0.1 g of syrup compound [E].

TLC data (developing solvent: chloroform: butanol: n-hexane = 85%: 10%: 5%): Rf = 0.08

¹ H-NMR spectral data δ: 2.35 (1H, t), 1.65 (1H, m), 1.25 (13H, m), 0.88 (2H, t)

IR spectral data IR (KBr), Cm-1: 3008, 2289 (C-H), 1652 (C = C), 1109, 1423, 1245

Example 11: Extraction process of [F]

Solvent fractionation based on the methanol extract (fresh fruit) 132g by the method according to Example 1 as shown in FIG. That is, the extract was extracted by adding 400 mL of dichloromethane in the fractional filter extract, and the insoluble compound was extracted by adding 300 mL of ethyl acetate. At this time, the insoluble compounds were combined and concentrated under reduced pressure, followed by column chromatography (developing solvent chloroform: methanol = 90: 10) to obtain 0.52 g of crystalline compound [F].

TLC data (developing solvent: chloroform: butanol: n-hexane = 85%: 10%: 5%): Rf = 0.03

IR Spectrum Data IR (KBr), Cm-1: 3010, 2990 (C-H), 1650, 1470 (C = C), 1710 (C = O)

Example 12 Extraction of [G]

After washing the figs cleanly, remove the water, add 1.5-10 times the amount of fig raw fruits to methanol based on 10kg of figs, extract them by immersion method at room temperature for about 2 months, and filter them by suction filtration with Bchner funnel. Is extracted, it was extracted up to about 20-70 days in order to increase the extraction efficiency. The extract was separated from the figs, and then methanol was removed using a vacuum concentrator (Bchi RE 121, Switzerland) at 30-60 ° C. to obtain fruit-323.5g and leaves-267.0g. As a sample, the solvent was fractionated as shown in FIG. That is, 200 ml of distilled water was added from the filtrate fraction of the fig extract, and extracted three times with 1 L of CHCl _{3. The} insoluble compound and the aqueous layer were extracted with saturated sodium chloride or saturated calcium chloride solution at room temperature with 300 mlX5 of saturated butanol, and the layers were separated. 1.0 g of magnesium sulfate was added thereto, the mixture was stirred well at 40 ° C. for 10 minutes, and the organic solvent was concentrated under reduced pressure. Finally, 28.4 g of a concentrate in the form of a liquid syrup was obtained. This was again performed by column chromatography (developing solvent = normal butanol: distilled water: chloroform = 10: 5: 80 and chloroform: methanol = 50:50) to give 10.3 g of colorless needles (G). This was measured by HPLC column and UV, and the absorption wavelength was 233 nm and melting point was 258-259 ° C.

TLC data (developing solvent: chloroform: butanol: n-hexane = 85%: 10%: 5%): Rf = 0.01

IR Spectrum Data IR (KBr), Cm-1: 2925, 28259 (-CH), 1721 (C = O), 1679 (C = CC = O), 1642 (C = C), 1542, 1549, 1387, 1262 , 1071, 771

Example 13: Extraction process of [G]

After washing the figs cleanly, the water was removed, and the figs were extracted by compressing them in the same manner as in Example 2 by adding 1.5-10 times the amount of each solvent with n-butanol, and filtering with Bchner funnel. After removing normal butanol, fruit-220g and leaf-212g were obtained. As a sample, the solvent was fractionated as shown in FIG. 1 based on 132 g of butanol raw fruit extract. That is, 200 ml of distilled water was added from the filtrate fraction of the fig extract, and extracted three times with 1 L of CHCl ₃ , and the insoluble compound and the aqueous layer were extracted with saturated sodium chloride or saturated calcium chloride solution at room temperature with 300 mlX5 of saturated butanol, and the layers were separated. 1.0 g of magnesium sulfate was added thereto, the mixture was stirred well at 40 ° C. for 10 minutes, and the organic solvent was concentrated under reduced pressure to obtain 28.4 g of a liquid syrup form. It was then subjected to column chromatography (developing solvent = normal butanol: distilled water: chloroform = 10: 5: 80 and chloroform: methanol = 50: 50) to give a solid compound [G].

TLC data (developing solvent: chloroform: butanol: n-hexane = 85%: 10%: 5%): Rf = 0.01

IR Spectrum Data IR (KBr), Cm-1: 2925, 28259 (-CH), 1721 (C = O), 1679 (C = CC = O), 1642 (C = C), 1542, 1549, 1387, 1262 , 1071, 771

Example 14 Extraction of [a]

After washing the figs cleanly, water was removed, and the fig leaves were extracted with the method of Example 1 by dipping at about room temperature for about 2 months by adding 1.5-10 times the amount of the solvent with methanol based on 10 kg of fig wet leaves. After removal, 100 g of the extract was taken and the solution was fractionated as shown in FIG. 2. That is, 200 ml of distilled water was added from the fig extract fraction filter, 150 ml of ethyl acetate was extracted five times, and the layers were separated. The aqueous layer was added with saturated NaCl solution and the pH of the aqueous layer was adjusted to 2.0. Then, 150 ml of ethyl acetate was extracted five times, and the layers were separated, dehydrated with anhydrous sodium sulfate, and the collected organic solvents were combined and concentrated under reduced pressure. Then, the mixture was extracted four times with 150 ml of CHCl _{3, and} concentrated under reduced pressure, followed by column chromatography (developing solvent = normal-butanol: distilled water: chloroform = 10: 5: 80 and chloroform: methanol = 50: 50) to give a solid compound [a]. 1.43 g was obtained.

TLC data (developing solvent: chloroform: butanol: n-hexane = 85%: 10%: 5%): Rf = 0.94

Example 15: Extraction process of [b]

By the method of Example 11 based on 100g extract was fractionated as shown in FIG. That is, 200 ml of distilled water was added from the extract extract filtrate, 150 ml of ethyl acetate was extracted five times, and the layers were separated. The aqueous layer was added with saturated NaCl solution and the pH of the aqueous layer was adjusted to 2.0. Then, 150 ml of ethyl acetate was extracted five times, and the layers were separated, decolorized with anhydrous sodium sulfate, and the organic solvents collected by filtration were concentrated under reduced pressure. It was extracted 4 times with 150 ml of CHCl ₃ again and concentrated under reduced pressure. Then, column chromatography (developing solvent = normal butanol: distilled water: chloroform = 10: 5: 80 and chloroform: methanol = 50:50) was performed to obtain 1.43 g of compound [b].

TLC data (developing solvent: chloroform: butanol: n-hexane = 85%: 10%: 5%): Rf = 0.89

IR spectral data IR (KBr), Cm-1: 1724 (CO), 1653, 1450 (C = C)

¹ H-NMR spectral data δ: 6.5 (1H, d, J = 9.6 Hz, H-3), 6.9 (1H, d, J = 3.2 Hz, H-3 '), 7.5 (1H, s, H -8), 7.8 (6H, s, H-5), 7.6 (1H, d, J = 1.7 Hz, H-2 ') 7.8 (H, d, J = 1.7 Hz, H-4)

Mass spectra showed ion peaks at m / z186, base peaks at m / z 131, and fragment ion peaks at m / z168.9, 158,147, and 118.9.

Example 16: Extraction process of [c]

By the method of Example 11 based on 100g extract was fractionated as shown in FIG. That is, 200 ml of distilled water was added from the fig extract fraction filter, 150 ml of ethyl acetate was extracted five times, and the layers were separated. The aqueous layer was added with saturated NaCl solution and the pH of the aqueous layer was adjusted to 2.0. Then, 150 ml of ethyl acetate was extracted five times, and the layers were separated, decolorized with anhydrous sodium sulfate, filtered, and the recovered organic solvents were combined and concentrated under reduced pressure. The mixture was extracted four times at room temperature and extracted with 150 ml of CHCl ₃ four times. Butanol: distilled water: chloroform = 10: 5: 80 and chloroform: methanol = 50: 50) was performed to obtain 0.1 g of the compound [c].

TLC data (developing solvent: chloroform: butanol: n-hexane = 85%: 10%: 5%): Rf = 1

IR spectrum analysis shows IR (KBr), Cm-1: 1732 (C = C), 1628, 1452 (C = C)

¹ H-NMR spectral data δ: 4.29 (3H, s, OCH ₃ ), 6.28 (1H, d, J = 9.8 Hz, H-3 '), 7.0 (1H, d, J = 2.3 Hz, H- 3 '), 7.3 (1H, s, H-8), 7.6 (1H, d, J = 2.4 Hz, H-2'), 8.17 (1H, d, J = 9.7 Hz, H-4)

Mass spectra showed ion peaks at m / z 216, base peaks at m / z 173.1, and fragment ion peaks at m / z 201.1, 188.1, 158.2, 145.2, and 131.1.

Example 17: Extraction process of [d]

By the method of Example 11 based on 100g extract was fractionated as shown in FIG. That is, 200 ml of distilled water was added from the fig extract fraction filter, 150 ml of ethyl acetate was extracted five times, and the layers were separated. The aqueous layer was added with saturated NaCl solution and the pH of the aqueous layer was adjusted to 2.0. Then, 150 ml of ethyl acetate was extracted five times, and the layers were separated, decolorized with anhydrous sodium sulfate, filtered, and the recovered organic solvents were combined and concentrated under reduced pressure. Again, 150 ml of CHCl ₃ was extracted four times, and the insoluble matter was adjusted to pH 2, and 250 ml of dichloromethane was extracted four times. The free organic layer was dehydrated by adding 1 g of anhydrous magnesium sulfate, and then concentrated under reduced pressure, followed by column chromatography (developing solvent). = Normal butanol: distilled water: chloroform = 10: 5: 80 and chloroform: methanol = 50: 50), and 0.1 g of compound [d] was obtained.

TLC data (developing solvent: chloroform: butanol: n-hexane = 85%: 10%: 5%): Rf = 0.68

IR Spectrum Data IR (KBr), Cm-1: 3500 (OH), 2983 (CH), 1650, 1400 (C = C)

¹ H-NMR spectral data δ: 1.0 (mH, m), 3.3 (1H, m, H-3), 5.3 (1H, m, H-6)

Mass spectra showed ion peaks at m / z 414 and fragment ion peaks at m / z 303, 254, 213, 159, 146, 107 and 95.

Example 18 Extraction of [e, f]

By the method of Example 11 based on 100g extract was fractionated as shown in FIG. That is, 200 ml of distilled water was added from the fig extract fraction filter, 150 ml of ethyl acetate was extracted five times, and the layers were separated. The aqueous layer was added with saturated NaCl solution and the pH of the aqueous layer was adjusted to 2.0. Then, 150 ml of ethyl acetate was extracted five times, and the layers were separated, decolorized with anhydrous sodium sulfate, filtered, and the recovered organic solvents were combined and concentrated under reduced pressure. The mixture was extracted four times with 150 ml each of CHCl _3, and the insoluble substance was adjusted to pH 2, and extracted again with 250 ml of dichloromethane four times. The insoluble compounds were separated by column chromatography (developing solvent = normal butanol: distilled water: chloroform = 10: 5). : 80 and chloroform: methanol = 50:50) to obtain 0.04 g of compound [e] and 0.12 g of compound [f], respectively.

TLC data (developing solvent: chloroform: butanol: n-hexane = 85%: 10%: 5%): Rf = 0.13

IR spectral data IR (KBr), Cm-1: 3300 (-OH), 1723 (CO), 1690 (C = O), 1650, 1450 (aromatic C = C)

¹ H-NMR spectral data δ: 6.2 (1H, d, J = 9.5, H-3), 6.8 (1H, s, H-8), 6.8 (1H, s, H-6), 7.7 (1H, d , J = 8.5, H-5), 7.87 (1H, d, J = 9.7 Hｚ, H-4), 10.5 (1H, s, OH)

Example 19: Extraction process of [g]

After washing the fig leaves, the water is removed and the fig leaves are extracted with methanol by adding 1.5-10 times the amount of solvent, based on 10 kg of fig wet leaves, to remove methanol using the method of Example 12, and then 100 g of the extract is extracted. The solution was fractionated as shown in FIG. That is, 200 ml of distilled water was added from the fig extract fraction filter, 150 ml of ethyl acetate was extracted five times, and the layers were separated. The aqueous layer was added with saturated NaCl solution and the pH of the aqueous layer was adjusted to 2.0. Then, 150 ml of ethyl acetate was extracted five times for 5 hours. The insoluble compounds and the aqueous layer were extracted with saturated sodium chloride or saturated calcium chloride solution at 300 mlX5 of saturated butanol at room temperature, and the layers were separated. Then, 1.0 g of magnesium sulfate was added and 40 Stir well at 10 ° C. for 10 min, filter it, and concentrate the organic solvent under reduced pressure to obtain 5.6 g of liquid syrup. This was further subjected to column chromatography (developing solvent = normal butanol: distilled water: chloroform = 10: 5:80 and chloroform: methanol = 50: 50) to obtain a compound [g].

Preparation Example 1

(Parts by weight)

Fig isolate component (A) 3.0 with antifungal and antibacterial properties

Glycerin 0.2

Polyethylene Glycol 4000 0.8

Soap Base 96.25

Tocopherol Acetate 0.3

Jojoba Oil 0.3

Cetanol0.15

The components were mixed well in a mixer and then extruded in a soap making machine, cut into a predetermined size, and molded to prepare a cosmetic soap composition in solid form.

Preparation Example 2

(Parts by weight)

Fig. Isolate A 13.0 with antifungal and antibacterial properties

Jojoba Oil 0.3

Soap Base 84.3

Tocopherol Acetate 0.3

Glycerin 0.2

Polyethylene Glycol 4000 0.8

Cetanol0.15

The components were mixed well in a mixer and then extruded in a soap making machine, cut into a predetermined size, and molded to prepare a cosmetic soap composition in solid form.

Preparation Example 3

(Parts by weight)

Fig. Isolate A 24.0 with antifungal and antibacterial properties

Glycerin 0.2

Polyethylene Glycol 4000 0.8

Jojoba Oil 0.3

Cetanol0.15

Soap Base 72.3

Tocopherol Acetate 0.2

The components were mixed well in a mixer, extruded in a soap making machine, cut and shaped into a recognized size to prepare a cosmetic soap composition in solid form.

Preparation Example 4

(Parts by weight)

Fig isolate ingredient with antifungal and antibacterial ingredients G 12.0

Polyethylene Glycol 4000 0.8

Jojoba Oil 0.3

Cetanol0.15

Soap Base 86.3

Butylated hydroxytoluene 0.05

Glycerin 0.2

The components were mixed in a mixer to prepare a cosmetic soap composition in the same manner as in Example 1.

Preparation Example 5

(Parts by weight)

Fig isolate with antifungal and antimicrobial ingredientsa 10.0

Butylated hydroxytoluene (B. H. T) 0.05

Glycerin 0.2

Polyethylene Glycol 4000 0.8

Soap Base 94.5

Tocopherol Acetate 2.0

Jojoba Oil 0.3

Cetanol0.15

The components were mixed in a mixer to prepare a cosmetic soap composition in the same manner as in Example 1.

Preparation Example 6

(Parts by weight)

Fig isolate containing antifungal and antibacterial ingredients a 3.0

Polyphenol 0.3

Glycerin 0.2

Polyethylene Glycol 4000 0.8

Soap Base 95.6

Tocopherol Acetate 0.7

Jojoba Oil 0.3

Cetanol0.15

The components were mixed in a mixer to prepare a cosmetic soap composition in the same manner as in Example 1.

Preparation Example 7

(Parts by weight)

Fig. Isolate A 20 with antifungal and antibacterial properties

Soap Base 5.0

Jojoba Oil 0.3

Cetanol0.15

Glutathione 1.0

Butylated hydroxytoluene 0.05

Tocopherol Acetate 0.3

Glycerin 0.2

D.B.A (alkylbenzene sulfonic acid) 0.4

The components were mixed in a mixer to prepare a shampoo composition in liquid form in the same manner as in Example 1.

Preparation Example 8

(Parts by weight)

Fig. Isolate A 15.0 with antifungal and antibacterial properties

Tocopherol Acetate 0.3

Glycerin 0.2

D.B.A (alkylbenzene sulfonic acid) 0.4

Soap Base 10

Oleic Acid 0.1

Cetanol 5

The components were mixed in a mixer and the water soap composition in liquid form was prepared in the same manner as in Example 1.

Preparation Example 9

(Parts by weight)

Fig. Isolates with antifungal and antibacterial ingredients (G, A) 5.0

Triamcinolone Acetite 0.1

Glycerin 0.2

Polyethylene Glycol 4000 0.8

Soap Base 91.3

Tocopherol Acetate 0.3

Jojoba Oil 0.3

Cetanol0.15

The components were mixed in a mixer to prepare a cosmetic soap composition in the same manner as in Example 1.

Preparation Example 10

(Parts by weight)

Fig separation component with antifungal and antibacterial ingredients (A) 20

Cetanol0.15

Tocopherol Acetate 0.3

Glycerin 0.2

Polyethylene Glycol 4000 0.8

The above ingredients were mixed in a mixer to prepare a liquid detergent in the same manner as in Example 1.

Preparation Example 11

(Parts by weight)

Fig leaf separation ingredient with antifungal and antibacterial ingredient G 5.0

Jojoba Oil 0.3

Cetanol0.15

Tocopherol Acetate 0.3

Glycerin 0.2

Polyethylene Glycol 4000 0.8

The above ingredients were mixed in a mixer to prepare a liquid rinse composition in the same manner as in Example 1.

Preparation Example 12

(Parts by weight)

Fig leaf separation ingredient with antifungal and antibacterial properties G, A 10.0

Jojoba Oil 0.3

Cetanol0.15

Tocopherol Acetate 0.3

Glycerin 0.2

Polyethylene Glycol 4000 0.8

The above ingredients were mixed in a mixer to prepare a liquid shampoo composition in the same manner as in Example 1.

Preparation Example 13

(Parts by weight)

Fig leaf separation ingredient G 4.0 with antifungal and antibacterial properties

Soap Base 10

Jojoba Oil 0.3

Cetanol0.15

Tocopherol Acetate 0.3

Glycerin 0.2

Polyethylene Glycol 4000 0.8

The above ingredients were mixed in a mixer to prepare a liquid shampoo composition in the same manner as in Example 1.

Preparation Example 14

(Parts by weight)

Fig leaf with antifungal and antibacterial ingredients

Soap Base 96.4

Jojoba Oil 0.3

Cetanol0.15

Tocopherol Acetate 0.3

Glycerin 0.2

Polyethylene Glycol 4000 0.8

The above ingredients were mixed in a mixer to prepare a cosmetic soap as a solid soap composition in the same manner as in Example 1.

Preparation Example 15

(Parts by weight)

Fig leaf with antifungal and antibacterial ingredients, fruit separation mixed ingredient (G, A) 20.0

Soap Base 78.4

Jojoba Oil 0.3

Cetanol0.15

Tocopherol Acetate 0.3

Glycerin 0.2

Polyethylene Glycol 4000 0.6

The above ingredients were mixed in a mixer to prepare a cosmetic soap formulation in the same manner as in Example 1.

Preparation Example 16

(Parts by weight)

Fig leaf with antifungal and antibacterial ingredients, separate component of fruit (G) 23.0

Soap Base 78

Jojoba Oil 0.3

Cetanol0.15

Tocopherol Acetate 0.3

Glycerin 0.2

Polyethylene Glycol 4000 0.8

The above ingredients were mixed in a mixer to prepare a cosmetic soap formulation in the same manner as in Example 1.

Preparation Example 17 (Comparative Example)

(Parts by weight)

Jojoba Oil 0.3

Soap Base 96.2

Glycerin 0.2

Polyethylene Glycol 4000 0.8

Cetanol0.15

The above ingredients were mixed in a mixer to prepare a soap composition in the same manner as in Example 1.

Preparation Example 18

(Parts by weight)

Fig. Isolate component (G) with antifungal and antibacterial properties 23

Soap Base 10.0

Oleic Acid 0.1

Cetanol0.15

Glutathione 1.0

Butylated hydroxytoluene 0.05

Tocopherol Acetate 0.3

Glycerin 0.2

D.B.A (alkylbenzene sulfonic acid) 0.4

The components were mixed in a mixer to prepare a shampoo composition in liquid form in the same manner as in Example 1.

Preparation Example 19

(Parts by weight)

Fig isolate ingredient with antifungal and antibacterial ingredients (G) 20

Oleic Acid 0.1

Cetanol0.15

Glutathione 1.0

Butylated hydroxytoluene 0.05

Tocopherol Acetate 0.3

Glycerin 0.2

The above ingredients were mixed in a mixer to prepare a cleaning composition in liquid form in the same manner as in Example 1.

Preparation Example 20

(Parts by weight)

Fig. Isolate component (A) with antifungal and antibacterial properties 25

Glutathione 1.0

Tocopherol Acetate 0.3

Glycerin 0.2

The ingredients were mixed in a mixer and an ointment composition in the form of a cream was prepared in the same manner as in Example 1.

Preparation Example 21

(Parts by weight)

Fig isolate ingredient with antifungal and antibacterial ingredients (G) 10

Glutathione 1.0

Tocopherol Acetate 0.3

Glycerin 0.2

The ingredients were mixed in a mixer and an ointment composition in the form of a cream was prepared in the same manner as in Example 1.

In order to confirm the fungal and antimicrobial therapeutic effects of the soap compositions prepared in Examples and Comparative Examples, an antimicrobial activity test and a clinical test to confirm the dandruff therapeutic effect were conducted.

Experimental Example 1: Determination of minimum fresh jersey concentration (MIC in vitro) for extracts of figs and fig leaves

1) Use strain

The microorganisms used in this experiment were G (-) bacteria, Escherichia coli ATCC 10536, Pseudomonas aeruginosa ATCC 9027, and G (+) bacteria Staphylococcus aureus ATCC 25923, Bacillus subtilis subtilis) ATCC 6633, Candida utilis, Candida albicans ATCC 10231, Saccharomyces cerevisiae ATCC 9763, Aspergillus niger 9029, Candida Candida pseudotropicalis, Tourlopsis glabrata 89-5 were used.

2) Medium used

Bacterial media used in this experiment: Mueller Hinton broth, Mueller Hinton agar and fungal media: Sabouraud Dextrose Broth, Sabouraud Dextrose agar.

3) Preparation of each fraction extract and separation substance, preparation of assay medium and measurement of antimicrobial activity:

① In order to make fungal diluent, pre-culture should be diluted to concentration of 104-106 CFU / ml.

② Dissolve each sample in a suitable solvent and dilute with 2 times dilution with medium.

③ Inoculate the bacteria solution in each test tube.

④ bacteria 37 degrees 24 hours incubation fungi 36-48 hours.

⑤ Shake the test tube after each incubation to check the turbidity of the bacteria. After checking the turbidity, each was inoculated in Mueller Hinton ager and Sabouraud Dextrose agar medium.

⑥ After confirming the growth of bacteria, determine the lowest test tube concentration at which growth is not recognized.

Minimum developmental inhibition concentrations (MIC, mg / in vitro for isolated compounds (A, B, C, D, E, F, G and a, b, c, d, e, f)) The results of measuring ml) are shown in Table 1 below.

Table 1.

Unit: mg / ml Strain G B C D E F A a b c d e K ^* Gram-positive bacteria Bacillus subtilis ATCC 6633 > 5.42 9 5 36 8 - 31 21 9 11.1 37 15 1.5 Staphylococcus aureus ATCC 25923 2.79 9.6 11 18.5 - - 11.5 11.3 9 5.56 18.5 15 7.5 Gram negative bacteria Escherichia coli ATCC 10536 > 2.79 4.5 13 13 5 10 13 11.3 4 5.56 18.5 15 1.87 Pseudomonas aeruginosa ATCC 9027 1.35 12.3 11 18 - - 20 20 12 2.28 18.5 15 1.87 Candida utilis 0.675 5 3.3 4.6 24.6 > 282 5 2.28 4.62 7.5 1.87 Candida albicans ATCC 10231 > 0.675 4 2.79 2.7 2.7 10 12.7 282 4 > 2.28 > 4.6 7.5 1.87 Saccharomyces cerevisiae ATCC 9763 2.79 4 7 18.5 - - 11.5 11.3 4 5.56 18.5 > 15 1.875 Aspergillus niger 9029 2.79 11.1 11 37 5 - 13.7 122.6 10 11.1 37 15 1.875

Note) K ^* represents ketoconazole.

The results of measuring the antibacterial activity of the medicinal soap composition of the present invention and the control soap or ketoconazole are shown in Table 2 below.

Table 2. Minimum Inhibition Concentration of Microorganisms

(Unit: μg / ml) Strain Preparation Example 7 (shampoo) Preparation Example 11 (rinse) Preparation Example 14 Preparation Example 17 (Control Soap) Ketoconazole Raw Material Escherichia coli ATCC 10536, 29 59 12 56 27.9 Pseudomonas aeruginosa ATCC 9027 15 25 10 28 13.5 Staphylococcus aureus as G (+) 35 37 15 60 27.9 Bacillus subtilis ATCC 6633, fungus 84 89 20 108 54.2 Candida utilis 6.0 5.0 1.5 14 6.75 Candida albicans ATCC 10231 3.5 4.5 2.0 14 6.75 Saccharomyces cerevisiae ATCC 9763 30 36 7 56 27.9 Aspergillus niger 9029 70 87 10 160 80 Candida pseudotropicalis 100 95 40 160 160 Tourlopsis glabrata 89-5 74 74 10 160 160

Note 1) The concentration of the control soap is based on the concentration of the fig separation component having antifungal and antibacterial ingredients at each dilution step of Example 7 shampoo, Example 11 ointment, and Example 14 soap.

Note 2) The dissolution of the raw material of fig isolate with antifungal and antibacterial ingredients is water.

Experimental Example 2: Confirmation of dandruff treatment effect and skin itching and skin nitriding such as athlete's foot and eczema

In order to measure the clinical effects of dandruff treatment and skin diseases such as itching and athlete's foot and eczema, 20 subjects in their 20s and 40s with high dermatitis such as dandruff and itching and athlete's foot and eczema (10 in experimental group and 10 in control group) ) Was selected as a test subject, and the soap composition of Preparation Example 1 (Experimental Group) and Control Soap (Preparation Example 7) of the present invention was used for scalp once a day for 2 weeks for dandruff and itching and athlete's foot eczema. The effects of back skin diseases were evaluated.

After determining the grade, the visual observation confirmed the reduction of dandruff, itching of the scalp, the itching of the skin, and the elimination of dermatitis such as athlete's foot and eczema.

Criteria are shown in Table 3 below.

Table 3.

Rating condition 0 1 2 3 4 The scalp does not completely itch and dandruff Slightly disappears the scalp itch and dandruff Significantly eliminates the itching and dandruff of the scalp The scalp virtually eliminates the itching and dandruff The scalp completely disappears

If the grade for the dandruff treatment of the clinical trial site shows a reduction rate of 3 to 4, the effect is excellent. The experimental results are shown in Table 4 below.

Table 4. Clinical trial results confirming dandruff treatment effect

Excellent therapeutic effect Treatment effect No therapeutic effect system Manufacture Example 1 Manufacture Example 11 Manufacture Example 13 Manufacture Example 17 (Comparative Example) 8 (90%) 9 (90%) 7 (90%) 4 (10%) 1 (10%) 3 (10%) 1 (10%) 000 9 (90%) Ten (100%) Ten (100%) Ten (100%) Ten (100%)

As shown in the table, the soap containing the anti-fungal and antibacterial fig separation component [A] was found to have a much better dandruff treatment effect than the soap does not contain it.

Experimental Example 3: Clinical trial of skin itching and dermatitis such as athlete's foot and eczema

Table 5. Criteria

Rating condition 1234 Itching of the skin and dermatitis such as athlete's foot and eczema are not completely disappeared.Itching of the skin and dermatitis such as athlete's foot and eczema. Dermatitis such as athlete's foot and eczema disappear completely

It is determined that the grade of skin itch, athlete's foot, eczema, and dandruff treatment of clinical trial site shows excellent effect when the reduction rate is 3 to 4, and the effect is medium when the reduction rate is 1 to 2, and no effect when the reduction rate is not shown. The experimental results are shown in the following table.

Table 6.

Excellent therapeutic effect Treatment effect No treatment effect system (Example 1) 8 (90%) 2 (10%) 0 10 people (100%) (Comparative Example 1) 0 1 (10%) 9 (90%) 10 people (100%)

As shown in the table, the ointment containing the anti-fungal and antimicrobial component of the fig separation component was found to have a much better skin healing effect such as itching and athlete's foot and eczema than the control ointment containing no.

The medicinal soap-free composition according to the present invention has excellent therapeutic effects such as skin athlete's foot and fungus and antibacterial without toxicity or side effects.

Claims (10)

Medicinal soap, characterized in that the alkali metal soap base contains 0.05 to 25% by weight of solvent extract isolates of fig fruit or leaves of Masui Dauphine, Bongsi or White Genoa Composition. The method of claim 1, wherein the extract of the fig fruit is extracted and separated fractions [A], [B], [C], [D], [E], [F] as shown in FIG. Or [G] medicinal soap composition. The extract of claim 1, wherein the extract isolate of the fig leaf is extracted and separated fractions [a], [b], [c], [d], [e], [f] as shown in FIG. Or [g]. A medicinal soap composition. The method of claim 1, wherein the solvent extract separation extract of the fig fruit or leaf by dipping, pressing or grinding method using a methanol solvent to obtain a mixed extract, which is an organic solvent ethyl acetate, n-butanol, ethyl ether , Extract with nucleic acid, chloroform, methylene chloride or water, respectively, to obtain a fraction extract, and also in a continuous method in an acidic solvent of pH 2-6.5 with saturated butanol or an alkaline solvent of pH 7.5-10 at a temperature of 30-90 ℃ The medicinal soap-free composition which is fractionated according to the type of solvent for 36 hours. The method of claim 1, wherein the extract separation of the fig fruit is decolorized by carbonization and the solution is neutralized with alkali and acid, which is an organic solvent, ethyl acetate, n-butanol, ethyl ether, nucleic acid, chloroform, methylene chloride or water. Separation was carried out to obtain a fractional active material, which was purified by silica gel (SiO ₂ ), alumina oxide (Al ₂ O ₃ ) and developing solvent (chloroform: butanol = 70: 30, methanol, acetonitrile, Ethyl acetate: chloroform: = 70: 30, n-butanol: chloroform: nucleic acid = 30: 60: 10, chloroform: methanol = 70: 30, n-butanol: distilled water: chloroform = 10: 5: 80 and chloroform: methanol = 50:50, chloroform: methanol = 90: 10, chloroform: methanol: ammonia water = 40: 40: 5). The extract of fig fruit or leaf is extracted with fig fruit by dipping the ripe fruit, immature fruit and leaf directly into n-butanol or methanol, extracting and concentrating under reduced pressure at a temperature of 20-70 ° C., and direct column chromatography A medicinal soapless composition which is separated into a mixture or a monocomponent compound by chromatography. 2. The stabilizer according to claim 1, wherein the stabilizer is vitamin C, tocopherol, glutathione, polyphenol, butylated hydroxyanisole, butylated hydroxytoluene, ascorbyl palmitate, ascorbyl oleate, sodium ascorbate, anhydrous sodium Bisulfite, anhydrous sodium sulfite, citric acid, sodium citrate, nitric acid, sodium carbonate, sodium bicarbonate, sodium thiosulfate, sodium bisulfite, thiourea, imideurea, polysorbate 80, polyoxymethylenenonylphenyl ether, oleic acid and D, B, A medicinal soap-free composition characterized in that one or two or more selected from A (alkylbenzenesulfonic acid). The medicinal soap composition according to claim 1, wherein the alkali metal base contains 7.0 to 28% by weight of water as an alkali metal salt of a fatty acid having 10 to 20 carbon atoms. The medicinal product according to claim 20, wherein the fatty acid is obtained from palm oil, palm oil, soybean oil, castor oil, olive oil, palm kernel oil, tallow, sunny, lard, or fish oil, and the alkali metal is neutralized by solely or mixing with sodium or potassium. Soap composition. The composition according to any one of claims 1, 2 and 3, wherein the composition is a shampoo, a liquid soap, a rinse, an antimicrobial agent, a fish disease treatment agent, a fish feed additive, a food processing additive, a pesticide substitute, an athlete's foot treatment agent, a skin ointment when planting a pollution-free plant. Or medicinal soap composition characterized in that the treatment for skin diseases.