KR102816565B1 - Liquid sample generating device for airborne viruses - Google Patents

Abstract

The present invention can provide a device for generating an aerosol sample from the air and then eluting the aerosol sample to generate a liquid sample in order to provide a liquid sample for an airborne virus to a virus detection sensor. The present invention can provide a device for efficiently separating particles of various sizes in the air using a cyclone device, and particularly for effectively capturing fine virus particles. The present invention provides a technology that integrates a process of converting a virus captured in the air into a liquid sample by combining it with an eluent, and can provide a device that utilizes a cyclone not only for simple separation of airborne particles but also for the elution step. The present invention can provide a device that detects the concentration of a sample in real time through a particle sensor, and provides a sample of an appropriate concentration to a virus detection sensor by adjusting the ratio of an eluent and deionized water, thereby increasing detection sensitivity.

Description

LIQUID SAMPLE GENERATING DEVICE FOR AIRBORNE VIRUSES

The present invention relates to a device for generating a liquid sample for an airborne virus. Specifically, the present invention relates to a device for generating an aerosol sample from air and then eluting the aerosol sample to generate a liquid sample, so as to provide a liquid sample for an airborne virus to a virus detection sensor.

Airborne viruses are one of the main causes of disease, transmitted through the respiratory tract. In particular, airborne viruses can spread rapidly in confined spaces, so the importance of technology to detect and detect them early is increasing significantly. The existing surface sampling method has limitations, so the need for technology to capture and analyze airborne viruses is increasing.

Currently, the representative technologies for capturing airborne particles include the filtering method and the cyclone method. The filtering method is a method of filtering airborne particles with a filter paper, and physically separates various particles according to size. On the other hand, the cyclone method is a method of separating heavy particles from the air flow using centrifugal force. However, the existing cyclone technology is mainly used to separate relatively large particles, and has limitations in effectively capturing fine particles such as viruses.

Viruses are very small, nanometer-sized particles that exist in the air. While conventional filtering and cyclone methods are suitable for handling relatively large particles, more precise capturing technology is needed to capture very small virus particles. Since the concentration of viruses in the air is low, it is difficult to obtain a sufficient amount of sample after capturing, so combining with concentration technology is essential for detecting them.

The novel method presented in the present invention not only uses cyclone technology to simply capture airborne particles, but also to elute captured viruses. The method of capturing airborne viruses through a cyclone device and converting them into a liquid sample by combining them with an eluent is a novel approach that is differentiated from existing technologies. In particular, in the present invention, the cyclone captures viruses and suspends them in an eluent to create a liquid sample.

A standard method for detecting a virus is to suspend the captured virus in a solvent to convert it into a liquid state, and then provide it to a biosensor or an analysis device. Typically, a solvent such as PBS (Phosphate Buffered Saline) is used to ensure that the captured virus is stably suspended in the liquid. The present invention provides a technology for effectively converting airborne viruses into liquid samples by combining cyclone capture technology with this method.

A sensor for virus detection detects a liquid sample containing captured viruses in real time to confirm the presence of the virus. Since the sensor requires very high sensitivity, it is important to optimize the concentration of the captured sample and deliver it. To this end, a technology is required to accurately deliver a liquid sample of an appropriate concentration by eluting the captured virus to the virus detection sensor. The present invention solves this problem by controlling the concentration of the eluent and providing an optimal sample.

The present invention provides an integrated system that converts airborne viruses into liquid samples by combining an airborne virus capture technology using a cyclone and an elution device. The process of separating particles of various sizes from the air using a cyclone, suspending the captured viruses in an elution solution, and ultimately delivering them to a virus detection sensor is organically performed. In this process, a fluid control technology that adjusts the concentration of the elution solution and optimizes the sample concentration is also applied.

The present invention proposes a new technology that improves the existing cyclone technology to effectively capture even minute virus particles in the air and elute them to convert them into a liquid sample. In particular, the method of utilizing the cyclone not only for simple separation of airborne particles but also for the elution process is a significant innovation of the present invention. The main purpose of the present invention is to increase the detection sensitivity of airborne viruses and implement a system capable of detecting viruses in real time.

Registered Patent No. 10-2630689 (Diagnosis of respiratory diseases using breath and aerosol analysis)

The present invention aims to solve the above-mentioned problems by achieving the following solutions.

The present invention aims to provide a device for generating an aerosol sample from air and then eluting the aerosol sample to generate a liquid sample in order to provide a liquid sample for an airborne virus to a virus detection sensor.

The purpose of the present invention is to provide a device that can efficiently separate particles of various sizes in the air using a cyclone device, and in particular, effectively capture fine virus particles.

The present invention is a technology that integrates a process of converting viruses captured in the air into a liquid sample by combining them with an eluent, and aims to provide a device that utilizes a cyclone not only for simple separation of airborne particles but also in the elution step.

The purpose of the present invention is to provide a device that detects the concentration of a sample in real time through a particle sensor and increases detection sensitivity by adjusting the ratio of a dissolution solution and deionized water to provide a sample of an appropriate concentration to a virus detection sensor.

The present invention aims to provide a device that converts airborne viruses into liquid samples and optimizes sample concentration to maximize detection efficiency by combining a dissolution device, a fluid control system, and a particle sensor.

The present invention aims to provide a device that prevents contamination during continuous processing and provides accurate and reliable samples, including an automated system capable of washing and disinfecting a collection surface with deionized water and ethanol after sample generation.

The problems solved by the present invention are not limited to those mentioned above, and other problems not mentioned can be clearly understood by a person having ordinary skill in the art from the description below.

According to various embodiments of the present invention, a liquid sample generating device for airborne viruses comprises: a cyclone device for removing particles from air to generate an aerosol sample; an elution device for generating a liquid sample from the aerosol sample; and a control device connected to the cyclone device and the elution device, wherein the cyclone device comprises: one or more cyclones for filtering the airborne particles; At least one particle sensor for detecting particle characteristics of particles introduced into the cyclone device and particles filtered by the at least one cyclone; wherein the at least one cyclone is connected in series, and the at least one cyclone is configured to filter particles of different particle sizes, respectively, and the at least one particle sensor includes an inlet particle sensor of an inlet through which air is introduced into the cyclone device and cyclone particle sensors corresponding to each of the at least one cyclones, and the elution device comprises: a collection surface which is a space where the aerosol sample is collected and an elution solution, which is phosphate buffered saline (PBS), is injected; an elution solution injection device for injecting the elution solution into the collection surface; a deionized water injection device for injecting deionized water (DI water) for controlling the concentration of the elution solution injected into the collection surface by diluting the elution solution into the collection surface; an ethanol injection device for injecting ethanol (EtOH) into the collection surface for disinfecting the collection surface; The aerosol sample comprises a filtering device for filtering particles larger than a predetermined size from the eluent or the mixed solution of the eluent and the deionized water; a sample collection unit for collecting the liquid sample of the mixed solution that has passed through the filtering device; a waste collection unit for collecting waste of the mixed solution filtered by the filtering device; a fluid control device for controlling the flow of the eluent, the deionized water, the ethanol, the mixed solution, and the liquid sample; and the control device comprises: a transceiver; a memory; an input device; an output device; and a processor functionally connected to the cyclone device, the eluent device, the transceiver, the memory, the input device, and the output device.

The present invention can provide a device for generating an aerosol sample from air and then eluting the aerosol sample to generate a liquid sample, in order to provide a liquid sample for an airborne virus to a virus detection sensor.

The present invention can provide a device that can efficiently separate particles of various sizes in the air using a cyclone device, and in particular, can effectively capture fine virus particles.

The present invention is a technology that integrates a process of converting viruses captured in the air into a liquid sample by combining them with an eluent, and can provide a device that utilizes a cyclone not only for simple separation of airborne particles but also in the elution step.

The present invention can provide a device that detects the concentration of a sample in real time through a particle sensor and increases detection sensitivity by adjusting the ratio of a dissolution solution and deionized water to provide a sample of an appropriate concentration to a virus detection sensor.

The present invention can provide a device that converts airborne viruses into liquid samples and optimizes sample concentration to maximize detection efficiency by combining a dissolution device, a fluid control system, and a particle sensor.

The present invention can provide a device that prevents contamination during continuous processes and provides accurate and reliable samples, including an automated system capable of washing and disinfecting a collection surface with deionized water and ethanol after sample generation.

The effects of the present invention are not limited to those mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the description below.

FIG. 1 illustrates an example of a detection system for airborne viruses according to an embodiment of the present invention.
FIG. 2 illustrates an example of a configuration of a liquid sample generating device for airborne viruses according to an embodiment of the present invention.
FIG. 3 illustrates an example of an airborne virus detection device implemented according to an embodiment of the present invention.
FIG. 4 illustrates an example of an airborne virus detection device implemented according to an embodiment of the present invention.
FIG. 5 illustrates an example of an airborne virus detection device implemented according to an embodiment of the present invention.
FIG. 6 illustrates an example of an elution device in an airborne virus detection device implemented according to an embodiment of the present invention.

Hereinafter, with reference to the attached drawings, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement the present invention. The present invention may be implemented in various different forms and is not limited to the embodiments described herein.

FIG. 1 illustrates an example of a detection system for airborne viruses according to an embodiment of the present invention.

Referring to FIG. 1, a detection system for airborne viruses according to an embodiment of the present invention consists of a cyclone, an elution unit, and a virus detection unit.

The detection system for airborne viruses according to an embodiment of the present invention comprises a cyclone device, an elution device, a virus detection sensor, and an air exhaust device. A detailed description of each component is as follows:

1. Cyclone Device

The cyclone device serves to separate and capture particles of various sizes in the air. In the present invention, the cyclone device includes one or more cyclones. When the one or more cyclones are multiple cyclones, the multiple cyclones are connected in series, and the multiple cyclones are composed of several cyclones connected in series, and each cyclone effectively separates particles of different sizes.

(1) Particle separation principle: The cyclone device rotates the air with centrifugal force, separating heavy particles (large particles) from the air flow and transferring light particles to the next cyclone. Through this process, even small particles such as viruses can be separated and captured in stages.

(2) Particle sensor: Each cyclone is equipped with a particle sensor that detects the size and concentration of particles in the air in real time. This allows the characteristics of the captured particles to be identified, and the amount and concentration of the appropriate eluent can be adjusted in the subsequent elution step.

2. Elution Device

The elution device is a device that suspends the virus particles captured through the cyclone in the elution solution and converts them into a liquid sample. At this stage, the viruses captured in the air are converted into a liquid state and prepared to be delivered to the virus detection sensor.

(1) Collection Surface: The captured virus particles are collected on the collection surface, and a dissolving solution such as PBS (Phosphate Buffered Saline) is injected onto this surface, so that the virus is suspended in the dissolving solution.

(2) Injection of eluent: Virus particles captured in the air are dissolved in the eluent to create a liquid state that can be analyzed. During this process, deionized water (DI water) is mixed into the eluent to control the concentration of the eluent, and the amount of eluent injected is automatically adjusted according to the particle concentration.

(3) Filtering device: Filters the eluted mixed solution to remove particles larger than a certain size. Unnecessary particles are removed from the liquid sample through a membrane filter or a rotating liquid impactor, and a high-purity liquid sample is produced.

(4) Lysis buffer injection device: A buffer solution injection device is included for mixing lysis buffer with the liquid sample generated through the filtering process. This device automatically mixes the filtered liquid sample with the lysis buffer to efficiently extract viral proteins and nucleic acids (RNA/DNA) in the sample. Through this process, the sample completes chemical treatment to increase the analysis sensitivity before being transmitted to the virus detection sensor.

(5) Washing and disinfection system: After the elution and filtering process is completed, a step is included to wash and disinfect the inside of the elution device using ethanol (EtOH) and deionized water, which prevents sample contamination and maintains reliable analysis.

3. Virus Detection Sensor

The virus detection sensor analyzes the liquid sample generated from the elution device to detect the presence of viruses. The sensor is a highly sensitive, high-sensitivity device that can detect even small amounts of virus particles in real time.

(1) Liquid sample provision: The liquid sample obtained from the elution device is delivered to the virus detection sensor, and a sample of an appropriate concentration is provided to optimize the detection sensitivity. To this end, the amount of the sample may be adjusted or a dilution process may be performed so that the sample particle concentration matches the reference concentration.

(2) Real-time detection: The virus detection sensor analyzes the virus concentration of the provided sample in real time and immediately determines whether the virus is present. This detection sensor is based on antibody or gene detection technology for a specific virus and can detect various viruses.

According to one embodiment of the present invention, a virus detection sensor may include: a reaction membrane for causing a chemical or biological reaction with a virus in the liquid sample; a water-permeable polymer layer of polyvinyl alcohol (PVA) provided on the reaction membrane; and a signal amplification pad positioned on the water-permeable polymer layer. A signal amplification buffer may be injected into the reaction membrane simultaneously with or sequentially from the antigen sample, and the water-permeable polymer layer may delay the release of a signal amplification material included in the signal amplification buffer.

According to one embodiment of the present invention, a virus detection sensor comprises: a sample pad; an absorbent pad; a membrane pad disposed between the sample pad and the absorbent pad; a conjugate pad disposed between the sample pad and the membrane pad; a TMB (Tetramethylbenzidine) pad disposed between the membrane pad and the conjugate pad; And a TMB strengthening pad disposed on the sample pad, wherein a test line of the membrane pad includes an antibody that specifically binds to any one of COVID-19 (CoV), AdV (Adenovirus), Influenza A, and Influenza B, the conjugate pad includes a platinum nanoparticle (Pt NP), the TMB strengthening pad is shorter than the sample pad, the TMB strengthening pad is disposed on one side of the sample pad, the TMB strengthening pad includes any one of citric acid, malic acid, maleic acid, and fumaric acid as an acid substance, and a concentration of the acid substance is 0.01 to 0.5 M, the TMB strengthening pad includes Na ₂ O ₂ , and a concentration of the Na ₂ O ₂ may be 10 to 100 mM.

According to one embodiment of the present invention, a virus detection sensor comprises: a support made of a single length member; a single sample pad disposed at one end of the support; a single absorbent pad disposed at the other end of the support; a first strip pad disposed on the support and positioned between the sample pad and the absorbent pad; a second strip pad disposed on the support and positioned between the sample pad and the absorbent pad, the second strip pad being spaced apart from the first strip pad; a first conjugate pad disposed between the sample pad and the first strip pad; And a second conjugate pad disposed between the sample pad and the second strip pad, wherein the first strip pad includes at least two first test lines, wherein the at least two first test lines include at least two antibodies that specifically bind to any one of COVID-19 (CoV), RSV, Influenza A, and Influenza B, the second strip pad includes at least two second test lines, wherein the second test lines include at least two antibodies that specifically bind to any one of COVID-19 (CoV), RSV, Influenza A, and Influenza B, the first conjugate pad includes at least two metal nanoparticles each bound to a different detection antibody, and the second conjugate pad may include at least two metal nanoparticles each bound to a different detection antibody.

4. Air Exhaust Device

The air exhaust device is designed to purify the air used within the virus detection device and safely exhaust it to the outside. This is designed to prevent potential contamination risks that may arise during the virus capture and elution process and to maintain the safety of the experimental environment.

(1) Air purification filter device: Purifies the air that has passed through the cyclone device and then the elution device. Removes residual particles, viruses, or other harmful substances in the air to create clean air.

(1-1) Composition: Effectively removes fine particles and viruses in the air, including a high-efficiency particulate air (HEPA) filter or an activated carbon filter. Purification efficiency can be maximized with a multi-stage filtering system (e.g., pre-filter + HEPA filter). Additionally, a plasma generation device can be included, and sterilization can be performed using plasma light.

(1-2) How it works: As air passes through the purifying filter, harmful substances are filtered out, and clean air moves to the exhaust line.

(2) Air exhaust port: Safely discharges purified air into the outside environment. Prevents additional contamination during the discharged air discharge process.

(2-1) Composition: The air outlet is designed to maintain smooth air flow and includes a backflow prevention device. A sound-absorbing structure is added around the outlet to minimize noise generated during operation.

(3) System Integration: The air exhaust device operates in integration with other components in the virus detection device. The air generated from the cyclone device passes through the elution device and is then purified through the air purification filter device before being discharged. The flow rate of the exhaust air, filter status, and discharge efficiency can be monitored in real time through the integrated control system.

(4) Summary of operating principles

(4-1) Air purification: Air drawn into the air exhaust device has viruses and harmful substances removed through a purification filter.

(4-2) Air exhaust: Purified air is exhausted to the outside through the exhaust port, preventing backflow and reducing noise during this process.

(4-3) Safety management: By monitoring the condition of the filter and automating the replacement or cleaning process when necessary, continuous purification performance can be maintained.

The operation process of the detection system for airborne viruses according to an embodiment of the present invention is as follows.

(1) Air intake and particle capture: Particles floating in the air are introduced into the cyclone device, and are separated and captured according to size as they pass through each cyclone. Particles of a certain size are captured in a form suitable for sensor analysis.

(2) Elution and filtering: The captured viruses are collected on the collection surface and suspended in the eluent injected here, thereby converting into a liquid sample. Subsequently, through a filtering process, unnecessary particles are filtered out, and a high-purity sample is generated.

(3) Lysis buffer mixing: The liquid sample generated through the filtering process is mixed with lysis buffer. This mixing process extracts viral proteins and nucleic acids (RNA/DNA) in the sample to maximize detection sensitivity.

(4) Virus detection: The generated liquid sample is transmitted to a virus detection sensor and analyzed in real time for the presence of viruses.

(5) Air purification and exhaust: The air used for virus detection is purified through an air purification filter device. The purified air is safely exhausted to the outside through an air outlet, ensuring prevention of contamination and backflow during this process.

A detection system for airborne viruses according to an embodiment of the present invention provides an integrated detection solution capable of capturing and analyzing viruses present in the air in real time, and is designed to effectively detect and respond to the spread of viruses in various environments.

FIG. 2 illustrates an example of a configuration of a liquid sample generating device for airborne viruses according to an embodiment of the present invention.

Referring to FIG. 2, a detection system for airborne viruses according to an embodiment of the present invention includes a liquid sample generating device (1000) and a virus detection sensor (2000).

A liquid sample generating device (1000) for airborne viruses according to an embodiment of the present invention includes a cyclone device (1100), an elution device (1200), a control device (1300), and an air exhaust device (1400).

Air outside the liquid sample generating device (1000) is introduced into the cyclone device (1100). The cyclone device (1100) provides an aerosol sample for airborne viruses to the elution device (1200). The elution device (1200) provides a liquid sample for airborne viruses to the virus detection sensor (2000).

The cyclone device (1100) includes a cyclone (1101) for large particles, a cyclone (1102) for medium-sized particles, a cyclone (1103) for small particles, a particle sensor (1104) for all particles entering the cyclone device (1100), a particle sensor (1105) for particles captured by the cyclone (1101) for large particles, a particle sensor (1106) for particles captured by the cyclone (1102) for medium-sized particles, and a particle sensor (1107) for particles captured by the cyclone (1103) for small particles. The cyclone device (1100) is a key device for separating and capturing particles of various sizes in the air, thereby efficiently separating airborne particles in a virus detection process. In Fig. 2, three cyclones (1101, 1102, 1103) are illustrated, but this is only an example and it is obvious that one or more different numbers of cyclones can be applied. In addition, it is obvious that one or more different numbers of particle sensors (1105, 1106, 1107) for the cyclones (1101, 1102, 1103) can also be applied. A detailed description of each component of the cyclone device (1100) is as follows.

The cyclone (1101) for large particles separates and captures large and heavy particles contained in the air. The cyclone (1101) for large particles removes relatively large particles (e.g., dust, pollen, etc.) from the air to purify the airflow. The large and heavy particles are separated by being pushed to the wall by centrifugal force. The large particle cyclone (1101) is mainly designed to capture particles larger than 10 micrometers (μm). This is the first step in purifying the airflow so that the remaining cyclones can handle smaller particles.

The cyclone (1102) for medium-sized particles processes medium-sized particles that have passed through the first cyclone. The cyclone (1102) for medium-sized particles captures relatively small particles (e.g., bacteria, fine dust, etc.) and prepares to capture small particles more effectively. It is an intermediate step for filtering medium-sized particles that are not removed by the first cyclone. The medium-sized particle cyclone (1102) is designed to capture particles mainly between 2 micrometers (μm) and 10 μm.

The cyclone (1103) for small particles is responsible for capturing the smallest particles in the air. The cyclone (1103) for small particles captures small particles of nanometer units (e.g., virus particles, etc.). It is the final step for detecting and separating fine particles such as viruses in the air. The small particle cyclone (1103) is mainly designed to capture particles of 1 micrometer (μm) or less. It plays an important role in separating small particles in the air.

The particle sensor (1104) for all particles detects all particles in the air flowing into the cyclone device (1100). The particle sensor (1104) for all particles detects and records the characteristics (size, concentration, mass, etc.) of particles in the air flowing into the cyclone device in real time. Through this, the entire distribution of particles flowing into the device can be determined. The particle sensor (1104) for all particles analyzes the size distribution, concentration, etc. of particles in the air in real time, and provides important data for controlling the system and adjusting the amount of solution injected.

The particle sensor (1105) for large particles is a sensor that detects particles captured by the large particle cyclone (1101). The particle sensor (1105) for large particles detects the number, size, and concentration of particles separated from the cyclone (1101) for large particles to determine whether the particles are properly captured. It provides data so that the system can adjust an appropriate amount of eluent depending on the characteristics of the captured particles.

The particle sensor (1106) for medium-sized particles is a sensor that detects particles captured by the medium-sized particle cyclone (1102). The particle sensor (1106) for medium-sized particles detects and records the number, size, and concentration of particles captured for medium-sized particles in real time. This allows the capture efficiency of medium-sized particles to be confirmed and adjustments to the eluent injection and filtering processes to be made.

The particle sensor (1107) for small particles is a sensor that detects fine particles captured by the small particle cyclone (1103). The particle sensor (1107) for small particles detects small particles in the nanometer unit and monitors the size and concentration of captured particles in real time. It is mainly used to determine whether fine particles such as viruses floating in the air have been captured.

When air is introduced into the cyclone device (1100), the particles are gradually separated while passing through cyclones (1101, 1102, 1103) for large particles, medium-sized particles, and small particles. The particle sensors (1105, 1106, 1107) corresponding to each cyclone monitor the characteristics of the captured particles in real time and provide data to the system. This data can be used to control the amount of eluent injected, the filtering efficiency, etc. This configuration plays an important role in effectively capturing particles of various sizes in the air and generating an accurate and reliable sample in the subsequent virus detection process. The cyclone device (1100) is optimized to efficiently separate particles of various sizes existing in the air and generate a liquid sample to be ultimately delivered to a virus detection sensor.

A particle sensor is a device that detects fine particles contained in air or liquid and measures their size, number, concentration, mass, etc. Particle sensors play an important role in various fields such as environmental monitoring, industrial process control, medical diagnosis, and virus detection. Generally, particles are detected using optical, electrical, or mechanical principles.

The particle sensor configuration is as follows.

(1) Light source: It plays a role in emitting light in the path that the particle passes through. Typically, a laser or LED light source is used to shine light on the particle to start the detection process. The light emitted from the light source interacts with the particle and is scattered, absorbed, or reflected.

(2) Detector: Detects the scattered light generated after particles interact with light, and calculates the size and number of particles. Typically, a photodiode, CCD, or CMOS sensor is used to detect changes in light and convert them into electrical signals.

(3) Flow Path or Measurement Chamber: The path through which particles flow, and is the section inside the sensor where particles interact with light. Particles are measured as they pass between the light source and the detector through this flow path. The flow path is designed to ensure a constant flow of particles, and provides an environment in which particles can sufficiently interact with the optical signal.

(4) Electronic circuit unit: A device that processes and analyzes the detected data, amplifies or filters the electrical signal generated by the sensor, and converts it into a digital signal. The detected signal is processed to produce meaningful data such as the size, number, and concentration of particles.

(5) Processor and memory: Processes, stores, or transmits data collected from sensors. The processor analyzes data in real time, and the memory can store data for a certain period of time. The processor analyzes the characteristics of particles through the collected data and generates appropriate control signals based on this.

The working principle of particle sensors is as follows. Particle sensors are generally based on optical principles and operate through the following process.

(1) Light emission and particle passage: A light source emits light, and particles interact with the light as they pass between the light source and the detector through the path. This interaction occurs in the form of light being scattered, reflected, or absorbed by the particles.

(2) Light scattering and detection: When particles scatter or absorb light, the resulting change in light is detected by a detector. Large particles scatter more light, and small particles scatter less. The detector converts this change into an electrical signal.

(3) Analysis of particle size and number: The detector analyzes the size of the particles by measuring the intensity of the scattered light. Large particles generate strong signals, and small particles generate weak signals, and the size and number of particles can be calculated based on this.

(4) Calculation of concentration and mass: Concentration can be measured by calculating how many particles there are per unit time or per unit volume. Also, since the mass of a particle is proportional to its size, the mass of the particle can also be inferred.

(5) Real-time data processing: The electronic circuitry and processor process data in real time to calculate the size, number, concentration, mass, etc. of particles, and transmit or store the results in the system.

The main functions of the particle sensor are as follows:

(1) Particle size detection: The size of particles can be measured by the degree of light scattering or absorption. Since the method of light scattering differs depending on the size, the particle size can be distinguished through this.

(2) Particle count measurement: The number of particles can be measured by analyzing the number or intensity of scattered light when the particle passes through the detection area. This is calculated by recording the frequency or intensity of light scattered by each particle.

(3) Concentration measurement: The concentration of particles can be calculated based on the number of particles detected per unit volume or time. This allows the density of particles in the air or liquid to be determined.

(4) Mass measurement: By combining particle size and concentration, the mass of the entire particle can be calculated. Since size and mass are proportional, the larger the size, the greater the mass.

(5) Real-time monitoring: Particle sensors detect particle characteristics and process data in real time, enabling immediate response and monitoring. This plays an important role in air quality monitoring, virus detection, etc.

The particle sensor works as follows:

(1) Air or liquid inflow: Air or liquid containing particles moves through the sensor's path.

(2) Interaction with light source: Particles interact with light from a light source, scattering or absorbing the light. The change in light that occurs during this process is detected by a detector.

(3) Signal conversion: The detected light change is converted into an electronic signal, which is used to calculate particle size, number, concentration, etc.

(4) Data processing: The processor processes data in real time and stores or transmits the results to the outside. This allows the characteristics of particles to be analyzed.

Application areas of particle sensors include:

(1) Air quality monitoring: Particle sensors are widely used to monitor the concentration of fine dust (PM2.5, PM10) and harmful particles in indoor and outdoor air.

(2) Virus detection: By measuring the size and concentration of virus particles in real time, the presence of viruses in the air or liquid can be determined.

(3) Industrial process control: Used to improve quality control by monitoring the concentration of impurities or fine particles in the manufacturing process.

(4) Medical and pharmaceutical fields: Used to analyze particles in drugs during pharmaceutical processes or to detect particles in medical equipment.

A particle sensor is a device that uses optical principles to detect and analyze microscopic particles in air or liquid. This allows the size, number, concentration, and mass of particles to be measured in real time, and plays an essential role in various industrial fields.

The elution device (1200) includes a collection surface (1201), an elution solution injection device (1202), a deionized water injection device (1203), an ethanol injection device (1204), a filtering device (1205), a sample collection unit (1206), a waste collection unit (1207), a fluid control device (1208), and a buffer solution injection device (1209). The elution device (1200) suspends viruses captured in the air in an elution solution to convert them into a liquid sample, filters the same, and then separates the sample and waste to provide a sample for virus detection. A detailed description of each component of the elution device (1200) is as follows.

The collection surface (1201) is a space where virus particles captured in the cyclone device (1100) gather, and is a surface where a dissolution solution is injected to suspend the virus particles in a liquid state. The collection surface (1201) is a surface where particles (especially viruses) captured in the air gather, and a dissolution solution (PBS) is injected to suspend the viruses in a liquid. This surface is optimized so that the particles can come into contact with the dissolution solution, thereby maximizing the suspension efficiency. The material of the collection surface does not react with the dissolution solution, and is designed to effectively collect the virus particles. It is mainly treated as a smooth surface so that the virus particles can be easily washed away by the dissolution solution.

The material and shape of the collection surface (1201) are important factors that determine the performance of the elution device, and play a key role in efficiently capturing fine particles such as viruses and maximizing contact with the elution solution to properly convert the sample. The material of the collection surface (1201) must be a smooth, inert material. The collection surface (1201) is designed to be a smooth material so that virus particles do not easily attach and are easily washed away by the elution solution. This material must be an inert material so that unnecessary chemical reactions do not occur between the particles and the elution solution. The materials commonly used for the collection surface (1201) include glass, polytetrafluoroethylene (PTFE), stainless steel, etc. Glass is inert and chemically stable, so it is often used for the collection surface. In addition, the smooth and soft surface of glass helps virus particles to be easily removed by the elution solution. PTFE has inert properties and high chemical resistance, so it minimizes reactions with the elution solution and prevents sample contamination. In addition, PTFE has a smooth surface, so viruses do not attach well. Stainless steel is durable and chemically stable, making it suitable for systems requiring high durability. However, stainless steel must have a smooth surface finish.

The collection surface (1201) must be able to withstand repeated elution processes, washing, and disinfection. Since it is repeatedly washed and disinfected with deionized water or ethanol, it must be durable enough not to wear out even after long-term use. The collection surface (1201) must be easy to wash and disinfect with ethanol and deionized water, and must be easy to maintain so that no residue remains.

The shape of the collection surface (1201) may be composed of a flat collection surface, a collection surface having an incline or curve, or a collection surface having micro-grooves.

The planar collection surface (1201) allows the virus particles to be evenly distributed and the eluent to flow easily. The planar collection surface is advantageous in that the eluent evenly covers the surface and effectively suspends the virus particles. The planar collection surface has a simple structure so that the eluent can evenly spread and come into contact with the virus particles when it flows. The planar collection surface can easily wash away the remaining particles after elution, making it easy to clean and disinfect. The planar collection surface is a surface type frequently used in laboratories, and has a structure that makes it easy to control the flow of the eluent.

The collection surface (1201) with a slope or curve helps the eluent to flow down naturally by gravity. The collection surface with a slope or curve makes the eluent better distributed and prevents particles from remaining on the surface, making the elution process more efficient. Since the collection surface with a slope or curve allows the eluent to flow down naturally without being accumulated on the collection surface, the particles can be suspended by contacting the eluent more efficiently. Even during washing, the eluent is easily discharged without remaining.

The collection surface (1201) with micro-grooves is designed to provide a location where particles can be fixed on the surface by adding micro-grooves or patterns to the surface while allowing the eluent to flow easily. This shape prevents the particles from flowing down and increases the contact time with the eluent, enabling efficient suspension. The collection surface with micro-grooves increases contact with the eluent while the micro-grooves temporarily hold the particles. This helps more particles to be suspended in the eluent, contributing to improving the eluent efficiency.

The collection surface (1201) should be designed so that the solution can be evenly spread over the collection surface and maximize contact with the particles. Therefore, the smoothness of the surface, the slope, the shape of the grooves, etc. are important factors that control the flow of the solution. The collection surface (1201) should be designed to have a structure that allows sufficient contact between the virus particles and the solution, and for this purpose, the shape and texture of the surface are important. The collection surface should be designed so that no residue remains after elution and that it can be easily washed and disinfected. This is an important factor that increases the reusability of the system and prevents contamination.

The collection surface (1201) is made of a smooth and inactive material to maximize virus capture and interaction with the eluent, and is designed to have a flat, inclined, or micro-grooved structure to allow the eluent to flow effectively. This design allows efficient elution of airborne virus particles, enabling accurate virus detection.

The eluent injection device (1202) is a device that suspends viruses by injecting eluent into the collection surface (1201). The eluent injection device (1202) injects eluent (PBS) into the collection surface to convert captured virus particles into a liquid state. The amount of eluent injected is adjusted according to the amount and characteristics of the collected particles. The amount and injection speed of the eluent in the eluent injection device (1202) are adjusted based on information obtained from the particle sensor, and the required volume of the eluent is precisely managed to optimize the sample concentration.

The DI water injection device (1203) is a device that injects DI water into the collection surface for adjusting the concentration of the eluent or for washing. The DI water injection device (1203) mixes the DI water with the eluent to adjust the concentration of the collected particle sample and injects it. It also serves to wash the collection surface after the elution to remove residues. In the DI water injection device (1203), DI water is used to dilute the concentration of the eluent and to wash the collection surface to prepare for the next sampling. The washing step prevents sample contamination and increases the reliability of the system.

The ethanol (EtOH) injection device (1204) is a device that injects ethanol to sterilize the system after the elution process. The ethanol (EtOH) injection device (1204) sterilizes the system after the elution process to prevent viruses or other contaminants from remaining. This is an essential process to prevent cross-contamination when reused. In the ethanol (EtOH) injection device (1204), ethanol is a strong disinfectant that completely sterilizes the system after the elution process to remove virus particles or impurities. The disinfection process plays an important role in increasing the reliability of the sample.

The filtering device (1205) filters out particles larger than a certain size from the eluted liquid sample. The filtering device (1205) purifies the sample using a membrane filter or a rotating liquid impactor. The filtering device (1205) filters out large particles (e.g., particles larger than 1 μm) from the eluted mixed liquid to obtain a pure virus sample. Only small particles such as viruses are transmitted to the detection sensor. The filtering device (1205) is used by combining one or both of a membrane filter and a rotating liquid impactor. The membrane filter allows only small-sized particles to pass through, and the rotating liquid impactor separates the particles using centrifugal force.

A Rotating Liquid Impactor is a device that collects fine particles floating in the air into a liquid and separates them according to their size or mass. A Rotating Liquid Impactor uses centrifugal force, or rotating force, to collect particles into a liquid and filter out particles of a desired size. A Rotating Liquid Impactor is used to filter particles in the air or prepare samples for analysis, and is particularly effective in capturing fine particles such as airborne viruses.

The configuration of the Rotating Liquid Impactor is as follows.

(1) Rotating disk: The rotating disk is a core component of the rotating liquid impactor, and is a disk that rotates at high speed. When particles suspended in PBS collide with the surface of the disk, they are separated according to size and mass. The rotating disk separates particles using centrifugal force, and effectively separates large and small particles through contact between the liquid and particles. When particles suspended in PBS collide with the rotating disk, the particles are separated according to size and move along a specific path.

(2) Inlet system: The inlet system is a system that introduces a liquid containing a PBS solution and particles into the rotating disk. The mixture is injected into the rotating disk at a constant speed, and the particles are separated by the rotating disk. The inlet system controls the inlet speed and amount of the liquid so that the mixture reaches the rotating disk at an appropriate ratio. This allows the particles to be properly separated and captured.

(3) Particle separation system: The particle separation system uses centrifugal force to separate particles suspended in PBS according to size and mass. Large particles are pushed outward, and small particles remain in the center and are separated. The size of the particles is separated according to the speed and structure of the rotating disk, and the separated particles move to the sample collection unit and the waste collection unit.

(4) Sample collection unit: The sample collection unit collects liquid containing small particles suspended in PBS solution. The liquid separated by particle size and mass is collected in the sample collection unit and delivered to the detection sensor. The sample collection unit collects a PBS mixture containing only small particles, and the liquid is then sent to the virus detection sensor.

(5) Waste treatment system: Liquid containing large particles is treated as waste. Large particles separated from the front disk and pushed outward flow into the waste collection unit. The waste collection unit collects and processes large particles and some PBS mixture, and discharges them through the waste treatment system when necessary.

The operating principle of the Rotating Liquid Impactor is as follows.

(1) Mixture introduction: A mixture of particles suspended in a PBS solution is introduced into a rotating disk through an introduction system. The liquid spreads over the rotating disk surface, and the particles come into contact with the disk surface.

(2) Particle separation by centrifugal force: Centrifugal force is generated when the rotating disk rotates at high speed. This centrifugal force pushes large particles outward from the disk, and small particles remain near the center. In this process, particles are separated by size.

(3) Particle collection and separation: Particles attached to the disk are separated according to size and mass. Large particles are pushed outward from the disk and moved to the waste collection section, while small particles remain in the center and are collected in the sample collection section. At this time, PBS solution is also distributed.

(4) Sample collection and provision: The PBS mixture containing small particles is delivered to the sample collection unit and then sent to the detection sensor. During this process, the sample collection unit provides a liquid containing only unfiltered small particles.

(5) Waste treatment: The PBS solution mixed with large particles is transferred to the waste collection unit and treated. If necessary, the waste can be discharged or treated for reuse.

Fine control of the Rotating Liquid Impactor is as follows.

(1) Rotation speed control: By controlling the speed of the rotating disk, the particle size can be accurately separated. When rotating quickly, large particles are separated more effectively, and when rotating slowly, small particles are captured better.

(2) Liquid inflow control: The amount and speed of the mixed liquid injected through the liquid inflow system can be controlled. If the injection amount is too large, particles may not be sufficiently captured, so the inflow should be done at an appropriate ratio.

A rotary liquid impactor is a device suitable for handling a mixture of particles suspended in PBS, and separates the particles by size through centrifugal force. This sends the PBS mixture containing only small particles to the sample collection section, and disposes of large particles as waste. This device is effective for handling not only airborne particles but also particles suspended in liquid.

The sample collection unit (1206) collects filtered liquid samples and delivers them to the virus detection sensor. The sample collection unit (1206) collects liquid samples that have been purified cleanly through a filtering process and is then provided to the virus detection sensor. During this process, the concentration of the sample must be optimized, and a sufficient amount required for detection is collected. The sample collection unit (1206) includes a device that can control the volume and concentration of the liquid sample, thereby providing the sample required for virus detection in an optimal state.

The waste collection unit (1207) is a component that collects waste remaining after filtering, that is, large particles or impurities. The waste collection unit (1207) separately collects and processes large particles or impurities remaining after filtering. This waste is appropriately processed so as not to interfere with the analysis. The waste collection unit (1207) is designed to collect and dispose of used eluent and impurities, and is managed so as not to cause cross contamination.

The fluid control device (1208) controls the flow of the solution, deionized water, ethanol, and sample liquid, and plays a key control role in the system. The fluid control device (1208) controls the injection amount of the solution, deionized water, ethanol, etc., and controls the flow of the liquid distributed to the sample collection unit and the waste collection unit. This allows the concentration of the sample to be accurately controlled, and provides the optimal sample required for detection. The fluid control device (1208) receives real-time data from the sensor, adjusts the flow of the solution and sample, and ensures that each liquid is injected at an accurate ratio. This allows the quality and concentration of the liquid sample to be consistently maintained.

The fluid control device (1208) is a key device that precisely controls the flow of various liquids such as the solution, deionized water, and ethanol in the elution device to ensure that the system performs optimally. This device regulates the flow of liquid, distributes liquid to the sample collection unit and the waste collection unit as needed, and controls the amount and ratio of liquid required at each injection stage.

The fluid control device (1208) is composed of various elements, which enable precise control of the flow of liquid within the system. The basic components are as follows.

(1) Fluid pump: A pump is used to deliver each liquid (solution, deionized water, ethanol, etc.) to the collection surface or filtering device. The pump controls the flow rate of the liquid and is precisely designed to provide various injection speeds.

(2) Valve system: Valves used to block or open the flow of fluid control the flow of liquid at various locations. This is used to control the distribution of liquid to the sample collection unit, waste collection unit, or to control the mixing ratio of the solution and deionized water.

(3) Flow meter: A flow meter is a device that measures the speed or amount of each liquid flowing. This allows it to detect in real time whether the liquid is flowing exactly as set and provides feedback.

(4) Sensor system: The fluid control device includes various sensors (particle sensors, pressure sensors, flow sensors, etc.) to monitor the flow of each liquid and its influence in real time. Based on this data, the processor can precisely control the liquid flow.

The operating principle of the fluid control device (1208) is basically to control the liquid flow through a processor and a sensor, and to adjust the eluent, deionized water, ethanol, etc. at the required ratio. The operating process is as follows.

(1) Data collection from sensors: Sensors such as particle sensors and flow meters detect liquid flow and particle concentration, size, etc. at each stage. Through this data, the fluid control device determines the amount and flow rate of each liquid.

(2) Processor control command: Based on the collected sensor data, the processor issues a command to adjust the flow of the liquid. For example, if the concentration of the sample particles is lower than the reference, a command is issued to increase the injection amount of the eluent or adjust the ratio of deionized water.

(3) Pump and valve operation: According to the command of the processor, the fluid pump injects the required amount of liquid, and the valve system adjusts the liquid to flow in the correct path. The pump controls the flow rate, and the valve opens or closes the flow of liquid to distribute the liquid to the sample collection unit and the waste collection unit.

(4) Real-time feedback control: By receiving real-time feedback from flow meters and sensors, the processor can continuously check and adjust whether the flow is as expected. If the solution is injected too fast or too slow, the pump speed is finely adjusted to compensate.

The fluid control device (1208) utilizes real-time communication and feedback loops between sensors and processors to control very precise liquid flow. This allows for fine adjustments to the liquid flow as follows:

(1) Flow control: The combination of a pump and a flow meter can be used to very precisely control the flow rate of the fluid. The flow meter measures the amount and speed of the liquid injected by the pump in real time and provides feedback to the processor so that it can adjust it. This feedback is repeated in very short cycles, so even small errors are immediately corrected.

(2) Mixing ratio control: The ratio of the solution and deionized water is controlled by the fluid control device, which can vary depending on the particle concentration or sample condition. Based on the particle size and concentration detected by the sensor, the processor adjusts the pump speed and valve opening degree to mix the two liquids at the desired ratio.

(3) Pressure and flow balance control: It is also important to balance the pressure within the system so that the fluid flows smoothly. Each valve and pump operates in synchronization to maintain the set pressure and flow rate, and the pressure sensor detects if this balance is broken and takes immediate action.

A specific operation example of the fluid control device (1208) is as follows.

(1) Sample collection and waste distribution: Depending on the size and concentration of the sample particles, the fluid control device controls the amount of liquid sample. When the sensor detects that the concentration of the sample particles is high, more eluent is sent to the sample collection unit and less liquid is sent to the waste collection unit. Conversely, when the sample concentration is low, more deionized water is injected to dilute it and then some of it is discharged to the waste collection unit.

(2) Washing and disinfection process: In the washing and disinfection step, the fluid control device precisely controls the flow of deionized water and ethanol to clean and disinfect the collection surface. This prevents cross-contamination and increases the reusability of the system.

The fluid control unit (1208) is a key device that precisely controls the flow of liquids such as eluent, deionized water, and ethanol through a real-time feedback loop of sensors and processors. This system, consisting of a pump, a valve, and a flow meter, efficiently controls the amount and ratio of liquids to maintain the virus sample captured from the air in an optimized state and increase detection sensitivity.

The buffer solution injection device (1209) is a device for accurately and uniformly mixing a lysis buffer into a liquid sample generated through a filtering process. This device precisely controls the mixing ratio of the sample and the buffer solution to maximize the virus detection sensitivity, and automates the chemical treatment process of the sample. The buffer solution injection device includes a fluid control system such as a quantitative pump to adjust the injection amount and flow rate according to the concentration of the sample and the analysis conditions. This allows efficient extraction of proteins and nucleic acids (RNA/DNA) of viruses in the sample, so that they can be prepared in an optimized state before being delivered to the virus detection sensor.

The operation of the elution device (1200) is as follows. The particles captured in the cyclone device (1100) are collected on the collection surface (1201) of the elution device (1200) and are suspended in the elution solution injected through the elution solution injection device (1202) and converted into a liquid sample. Thereafter, large particles are sent to the waste collection unit (1207) through the filtering device (1205) and small particles are collected in the sample collection unit (1206). The liquid sample collected in the sample collection unit (1206) is transferred to a virus detection sensor and analyzed. In this process, a lysis buffer is mixed into the sample through the buffer solution injection device (1209) to extract virus proteins and nucleic acids and maximize the detection sensitivity. After the work is completed, the collection surface is washed through the deionized water injection device (1203) and the system is disinfected through the ethanol injection device (1204) to prevent cross-contamination. The elution device (1200) is designed as a high-efficiency system that effectively captures airborne viruses and converts them into optimized liquid samples.

The control device (1300) includes a transceiver (1301), a memory (1302), a processor (1303), an input device (1304), and an output device (1305). The control device (1300) controls the cyclone device (1100), the elution device (1200), and the air exhaust device (1400), and plays an important role in coordinating the operation of the entire system, such as liquid flow, filtering, and sample distribution.

The transceiver (1301) is responsible for transmitting and receiving data between the control device and an external system or internal component. The transceiver (1301) receives data from various sensors and external devices, and transmits commands processed by the processor to other components. The transceiver (1301) collects real-time data from the sensor and transmits it to the processor. In addition, it transmits commands processed by the processor (1303) to a solution injection device, a fluid control device, a particle sensor, etc., and plays an important role in controlling the liquid sample generation device (1000). Through the transceiver (1301), the control device (1300) receives particle size or concentration from the sensor in real time, and issues commands to adjust the solution injection amount, buffer injection amount, etc.

The memory (1302) is a device that stores data and commands processed by the control device (1300). It temporarily stores data collected in real time, or records commands and setting values that can be preserved in the long term. The memory (1302) stores data necessary for the liquid sample generation device (1000) to operate normally, and allows the processor (1303) to access the necessary data at any time. It records operational data such as particle size, concentration data, and injection speed and ratio of each liquid collected in real time, so that the processor (13030) can analyze and process them whenever necessary. The memory (1302) stores set reference values (e.g., injection ratio of eluent, reference value for particle size, injection amount of buffer solution, etc.), and compares and processes data detected in real time.

The processor (1303) acts as the brain of the control device, and plays a central role in processing and analyzing collected data and issuing various control commands. It analyzes data transmitted from sensors to command necessary adjustments or performs system control according to user input. The processor (1303) controls the operation of the liquid sample generation device (1000) based on sensor data and controls the amount of solution injected, particle filtering, amount of buffer injected, washing and disinfection processes, etc. in real time. The processor (1303) also compares real-time data with reference values stored in the memory to control the concentration of the solution or manages the efficient operation of the liquid sample generation device (1000). When the particle concentration is higher than the set reference, the processor (1303) increases the amount of solution injected or issues a command to send more liquid to the sample collection unit.

The input device (1304) is an interface that allows a user to input commands to the system or change setting values. The user can control various operations of the system or set the injection ratio of the solution, the washing cycle, the disinfection cycle, etc. through the input device. The input device (1304) is configured in the form of a keypad, a touch screen, or a button, and the user can control the system or set parameters through this. The values set by the user are transmitted to the processor and used to adjust the system operation. The user can manually set the injection ratio of the solution or change the filtering cycle through the input device (1304). In addition, the user can adjust the washing and disinfection cycle according to specific sample conditions through the input device (1304).

The output device (1305) is a device that displays the status of the liquid sample generation device (1000), sensor data, processing results of the processor, etc. to the user. It allows the user to visually check the current status of the liquid sample generation device (1000) and also serves to convey warning or error messages. The output device (1305) is configured in the form of an LED display, an LCD panel, or a warning light, and displays the status or result data of the liquid sample generation device (1000) in real time. Through this, the user can check whether the liquid sample generation device (1000) is operating properly or whether a problem has occurred. The output device (1305) shows the current particle concentration, solution injection amount, buffer solution injection amount, and system warnings (e.g., filter clogging, solution shortage, etc.) to the user in real time. In addition, data that can confirm whether the liquid sample generation device (1000) is operating properly is output.

The control device (1300) collects real-time data from the sensor, and based on the data, the processor determines the optimal liquid flow, solution concentration, washing and disinfection cycle, etc., to control the liquid sample generation device (1000). Data is exchanged with an external device through a transceiver, and it is confirmed whether the liquid sample generation device (1000) is operating normally by comparing it with a reference value stored in a memory. The user can control the liquid sample generation device (1000) or change the setting value through the input device, and can check the status of the liquid sample generation device (1000) through the output device.

The operation process of the control device (1300) is as follows.

(1) Data collection: Data collected from particle sensors and flow meters, etc. are transmitted to the processor via a transmitter/receiver.

(2) Data analysis and processing: The processor compares the collected data with the reference values in the memory and issues appropriate control commands.

(3) Liquid flow control: The solution injection device and fluid control device control the liquid flow according to the command of the processor.

(4) Real-time feedback: Transmits real-time data from the system to an output device so that the user can check the status of the system.

(5) User control: Users can control the operation of the system or set specific values through input devices.

The control device (1300) plays an essential role in stably and precisely managing the entire liquid sample generation device (1000), and each component interacts with each other to optimize the performance of the airborne virus detection system.

The air exhaust device (1400) includes an air exhaust port (1401) and an air purification filter device (1402). The air exhaust device (1400) purifies the air used inside the virus detection system and safely discharges it to the outside. This prevents fine particles, residual viruses, or other harmful substances that are not captured in the air flow inside the system from leaking out, and ensures the safety of the working environment and the reliability of the system. The air exhaust device is composed of an air purification filter device (1402) that removes particles in the air and an air exhaust port (1401) that smoothly discharges purified air to the outside, thereby efficiently integrating the purification and discharge processes.

The air exhaust port (1401) is designed as a passage for safely discharging purified air to the outside. This component controls the direction and flow of the air discharge, and includes a backflow prevention device to prevent contaminated air from flowing back. The exhaust port is designed to optimize the speed and direction of the air flow to increase the discharge efficiency, and to ensure that the discharge to the outside environment is uniform. In addition, a sound-absorbing structure can be additionally applied to reduce noise during the discharge process, thereby improving the convenience of the working environment.

The air purification filter device (1402) is a key component that purifies the air in the virus detection system. The air purification filter device (1402) removes fine particles, viruses, and harmful substances in the air, including a high-efficiency particulate air (HEPA) filter and an activated carbon filter. The HEPA filter provides a performance capable of filtering out more than 99.97% of fine particles of nanometer size, and the activated carbon filter improves air quality by adsorbing harmful gases. The purification filter is designed in multiple stages to perform particle removal and chemical purification processes simultaneously, and is designed to be easy to replace and maintain periodically. The air purification filter device (1402) may further include a plasma air purification device that purifies the air by generating plasma.

According to various embodiments of the present invention, a liquid sample generating device for airborne viruses includes a cyclone device for removing particles from air to generate an aerosol sample; an elution device for generating a liquid sample from the aerosol sample; an air exhaust device for exhausting air that has passed through the elution device after being introduced through the cyclone device to the outside; and a control device connected to the cyclone device, the elution device, and the air exhaust device.

According to various embodiments of the present invention, the cyclone device includes: one or more cyclones for filtering particles in the air; one or more particle sensors for detecting particle characteristics of particles introduced into the cyclone device and particles filtered by the one or more cyclones. The one or more particle sensors include an inlet particle sensor at an inlet through which air is introduced into the cyclone device and one or more cyclone particle sensors corresponding to each of the one or more cyclones. When the one or more cyclones are a plurality of cyclones, the plurality of cyclones are connected in series. The plurality of cyclones are configured to filter particles having different particle sizes, respectively.

According to various embodiments of the present invention, the elution device includes: a collection surface, which is a space where the aerosol sample is collected and an elution solution, which is phosphate buffered saline (PBS), is injected; an elution solution injection device for injecting the elution solution into the collection surface; a deionized water injection device for injecting deionized water (DI water) for diluting the elution solution into the collection surface to adjust the concentration of the elution solution injected into the collection surface; an ethanol injection device for injecting ethanol (EtOH) into the collection surface for disinfecting the collection surface; a filtering device for filtering particles having a predetermined size or larger from a mixture in which the aerosol sample is eluted with the elution solution or the elution solution and the deionized water; a sample collection unit for collecting a liquid sample that has passed through the filtering unit among the mixture; a waste collection unit for collecting waste filtered by the filtering unit among the mixture; A buffer solution injection device for mixing a buffer solution, which is a lysis buffer, with the liquid sample; a fluid control device for controlling the flow of the eluent, the deionized water, the ethanol, the mixed solution, the liquid sample, and the buffer solution;

According to various embodiments of the present invention, the air exhaust device includes: an air purification filter device for purifying the air that has passed through the elution device after being introduced through the cyclone device; and an air outlet for discharging the air that has passed through the air purification filter to the outside.

According to various embodiments of the present invention, the control device includes: a transceiver; a memory; an input device; an output device; and a processor functionally connected to the cyclone device, the elution device, the air exhaust device, the transceiver, the memory, the input device and the output device.

According to various embodiments of the present invention, the processor may be configured to: determine a ratio and amount of the solution and the deionized water to be injected into the collection surface according to the particle characteristics detected by the one or more particle sensors; and inject the solution, or the solution and the deionized water, into the collection surface based on the determined ratio and amount by the solution injection device and the deionized water injection device.

According to various embodiments of the present invention, the particle characteristics may include one or more of particle size, particle concentration, particle mass, particle number, and particle collection efficiency. The particle concentration may be the number of particles per unit volume. The particle mass may be the total mass of particles captured by the one or more cyclones. The particle number may be the total number of particles captured by the one or more cyclones. The particle collection efficiency may be the ratio of the number of particles captured by the one or more cyclones to the total number of particles entering the plurality of cyclones.

According to various embodiments of the present invention, based on the particle characteristics, the processor: if the number of particles larger than a reference size among the particles captured by the one or more cyclones is greater than the number of particles smaller than the reference size, the ratio of the eluent is increased and the ratio of the deionized water is lowered; if the particle concentration of the particles captured by the one or more cyclones is higher than the reference concentration, the amount of the eluent is increased, the ratio of the eluent is increased and the ratio of the deionized water is lowered; if the total mass of the particles captured by the one or more cyclones is higher than the reference mass, the amount of the eluent is increased, the ratio of the eluent is increased and the ratio of the deionized water is lowered; if the total number of the particles captured by the one or more cyclones is higher than the reference number, the amount of the eluent is increased, the ratio of the eluent is increased and the ratio of the deionized water is lowered; Alternatively, if the particle collection efficiency is higher than the reference efficiency, the method may be further configured to increase the amount of the eluent, increase the ratio of the eluent, and decrease the ratio of the deionized water.

According to various embodiments of the present invention, the filtering device may be configured with one of a rotating liquid impactor or a membrane filter, or a combination of the rotating liquid impactor and the membrane filter. The rotating liquid impactor may be configured to rotate the mixed liquid so that, depending on the size of the particles, large particles are captured in the liquid and small particles are discharged. The membrane filter may be configured so that large particles are captured in the membrane filter and small particles are discharged through the pores of the membrane filter. When the filtering device is configured with a combination of the rotating liquid impactor and the membrane filter, the filtering device may be configured so that the rotating liquid impactor filters particles that are relatively larger than the membrane filter and then the membrane filter filters particles that are relatively smaller than the rotating liquid impactor.

According to various embodiments of the present invention, the mixture may be composed of a solid component and a liquid component. The solid component may include sample particles having a size smaller than a reference size that pass through the filtering device and are sent to the sample collection unit, and waste particles having a size larger than the reference size that are filtered by the filtering device and are sent to the waste collection unit. The liquid component may include the eluent injected into the collection surface, or the eluent and the deionized water. The liquid component may be distributed to the sample collection unit and the waste collection unit through a fluid distribution valve by the fluid control device based on a set distribution ratio.

According to various embodiments of the present invention, the distribution ratio can be based on the purity of the liquid sample or the efficient use of the eluent. When the distribution ratio is based on the purity of the liquid sample, the distribution ratio can be set such that the ratio of the liquid distributed to the sample collection unit is higher than a reference sample liquid ratio and the ratio of the liquid distributed to the waste collection unit is lower than a reference waste liquid ratio. When the distribution ratio is based on the efficient use of the eluent, the distribution ratio can be set such that the ratio of the liquid distributed to the sample collection unit is lower than the reference sample liquid ratio and the ratio of the liquid distributed to the waste collection unit is higher than a reference waste liquid ratio.

According to various embodiments of the present invention, the processor may be further configured to: wash the collection surface by injecting the deionized water into the collection surface by the deionized water injection device after the distribution of the mixed solution to the sample collection unit and the waste collection unit is completed; and disinfect the collection surface by injecting the ethanol into the collection surface by the ethanol injection device after the collection surface is washed.

According to various embodiments of the present invention, the processor may be further configured to: after distribution of the mixed liquid to the sample collection unit and the waste collection unit is completed, discharge a set amount of the provided sample among the liquid samples in the sample collection unit to a virus detection sensor outside the liquid sample generating device by the fluid control device.

According to various embodiments of the present invention, the sample collection unit may include a liquid particle sensor. The liquid particle sensor may be configured to detect one or more of the number or concentration of the sample particles in the liquid sample within the sample collection unit. The concentration of the particles may be based on the volume of the liquid sample within the sample collection unit and the number of the sample particles. The volume of the liquid sample may be based on the volume dispensed to the sample collection unit of the liquid composition. The amount of the provided sample may be determined by the processor based on the concentration of the sample particles. Based on whether the concentration of the sample particles is within a set reference sample concentration range, is higher than the reference sample concentration range, or is lower than the reference sample concentration range, the amount of the provided sample may be set to the reference sample amount, is set to be higher than the reference sample amount, or is set to be lower than the reference sample amount. The extent to which the provided sample amount is set higher or lower than the reference sample amount may be based on a ratio of the concentration of the sample particles to a median value of the reference sample concentration range.

Liquid particle sensors are devices that detect the size, number, and concentration of particles in liquids in real time, and are used to accurately detect and analyze fine particles in particular. These sensors can measure the characteristics of particles contained in liquids in various environments, and play an important role in virus detection systems, pharmaceuticals, life sciences, and industrial processes.

The configuration of the liquid particle sensor is as follows.

(1) Light source: An optical method is used to detect particles in a liquid, and light is generally emitted into the liquid using a light source such as a laser or LED. The light from the light source passes through the liquid and hits the particles, and the size or concentration of the particles is measured by utilizing the characteristics of being scattered or absorbed by the particles.

(2) Detector: A device that detects the reflected or scattered signal after the light emitted by the light source interacts with the particle. The detector converts the optical signal into an electrical signal and analyzes the size and number of particles based on the degree to which the particle scatters or absorbs light. High-sensitivity devices such as photodiodes or CCD sensors can be used.

(3) Flow Cell: A passage through which liquid flows, designed to allow the liquid to pass between a light source and a detector. While the liquid passes through this passage, particles are irradiated by the light source and detected by the detector. The flow cell is made of a transparent material so that the particles can interact accurately with the light, and is designed to allow the particles to flow freely. Since the liquid must flow regularly in a fixed flow cell, a pump or valve that controls the flow of the liquid may also be used.

(4) Electronic circuit unit: This is a device that analyzes the signal received from the detector and calculates the size, number, and concentration of particles through this. It converts the electric signal according to the scattering intensity, size, and concentration of the particles into a digital signal and processes it. A highly sensitive circuit that can detect very small voltages or currents is required, and processes this data to provide the final analysis results. This circuit unit may also include a memory and communication device that are responsible for storing and transmitting data.

(5) Processor and Software: The central processing unit (CPU) and its associated software are used to process and interpret the detected data. The data is processed in real time, and the size distribution, number, and concentration of particles are calculated to provide the user with the results. The software visually expresses the data through a graphical interface so that the user can easily understand it.

The working principle of the liquid particle sensor is as follows.

(1) Light scattering and particle detection: When particles in a liquid interact with light emitted from a light source, the light is scattered or partially absorbed by the particles. This light scattering varies depending on the size, number, and concentration of the particles. Large particles scatter more light, and small particles scatter relatively less. The detector measures the amount of this scattered light to analyze the size and concentration of the particles.

(2) Particle size analysis: The detector measures the intensity of the scattered light, and estimates the particle size from this. It works in such a way that large particles generate strong signals, and small particles generate weak signals. Based on this, the sensor calculates the particle size distribution in the liquid. If there are particles of various sizes, the scattered signal is different for each size, and this is used to obtain the distribution.

(3) Measurement of the number and concentration of particles: Based on the frequency and intensity of the detected signal, the number of particles present in the liquid can be calculated. If the intensity of the scattered light is generated from particles of a certain size, the number of particles of that size can be estimated by measuring the frequency of the signal. Concentration is defined as the number of particles per unit volume, and the concentration can be calculated by comparing the volume of liquid passing through the passage with the number of particles.

(4) Real-time data processing: The electronic circuitry and processor process the detection data in real time to calculate the size, number, and concentration of particles in the liquid and provide the results to the user. If necessary, the data can be stored or transmitted to an external system via a communication device.

The working process of the liquid particle sensor is as follows.

(1) Liquid flow inflow: Liquid flows through the channel, and particles flowing within the channel are irradiated by a light source.

(2) Light scattering: When particles contained in a liquid interact with light, scattered light is generated, and the detector detects this in real time.

(3) Signal conversion: The scattered light is converted into an electronic signal, and the size and number of particles are measured based on the intensity and frequency.

(4) Data processing: The electronic circuitry and processor process signals in real time to calculate the size, number, and concentration of particles and provide the data to the user.

Liquid particle sensors can be used in a variety of fields. For example:

(1) Virus detection: Used to measure the size and concentration of virus particles suspended in PBS in real time in a virus detection system.

(2) Pharmaceutical industry: It plays an important role in detecting and controlling impurities in liquids in pharmaceutical processes.

(3) Life science: Used to analyze biological samples such as cells, viruses, and protein particles.

(4) Water quality monitoring: Used to monitor water quality and detect minute impurities.

Liquid particle sensors are devices that can precisely measure the size, number, and concentration of particles contained in a liquid, and analyze the characteristics of particles in real time using a light source and detector. They play an important role in various fields such as virus detection, pharmaceutical process management, and water quality monitoring, and are particularly useful for accurately detecting and analyzing fine particles.

FIG. 3 illustrates an example of an airborne virus detection device implemented according to an embodiment of the present invention.

Figure 3 illustrates a side view of an airborne virus detection device of the present invention, showing the main components of the device. A cyclone device separates and captures airborne particles by size, and the captured particles are transferred to an elution device.

The elution device serves to convert the virus particles transferred from the cyclone device into a liquid sample. The device includes a collection surface and an elution solution injection device, and maximizes the sensitivity of virus detection through the purification of the liquid sample.

The collection surface is a key component where virus particles gather, and the virus is eluted through interaction with the eluent. The liquid sample generated through this process is transferred to the sample collection unit to complete the detection preparation.

The air outlet of the virus detection device discharges purified air to the outside, preventing airborne contaminants from leaking out. The air outlet is designed to maintain a smooth airflow.

FIG. 4 illustrates an example of an airborne virus detection device implemented according to an embodiment of the present invention.

Figure 4 illustrates another side view of the airborne virus detection device, highlighting the system integration design including the control unit. The control unit manages all components of the device in an integrated manner.

The cyclone device gradually separates particles of different sizes in the air and passes each separated particle to the elution device. This enables efficient handling of various particle sizes in the air.

At the top of the elution device, there are several injection devices, which support sample preparation and device sterilization through injection of eluent, deionized water, ethanol, etc., respectively. This process contributes to preventing cross-contamination and maintaining sample quality.

The control unit interacts with the user via a touch screen display, allowing real-time monitoring and manipulation of the status of each component, thereby increasing the efficiency and usability of the device.

FIG. 5 illustrates an example of an airborne virus detection device implemented according to an embodiment of the present invention.

Figure 5 illustrates a top view of the airborne virus detection device of the present invention, showing the physical arrangement of all components at a glance. The interconnection of the cyclone device, the elution device, and the air exhaust port is clearly shown.

The cyclone device is a multi-stage centrifugal force-based system for separating and capturing airborne particles, with particle size being captured at each stage. This plays a key role in increasing detection sensitivity.

The upper part of the elution device is equipped with various injection devices, each of which supports the elution process and the washing and disinfection process. This ensures the continuous performance of the elution device.

The air outlet discharges purified air to the outside together with the air purification filter device, maintaining the safety and environmental friendliness of the virus detection device. The outlet smoothly regulates the flow of purified air, providing stability to the entire system.

FIG. 6 illustrates an example of an elution device in an airborne virus detection device implemented according to an embodiment of the present invention.

FIG. 6 is an example illustrating the main components of an elution device in an airborne virus detection device according to an embodiment of the present invention. The elution device serves to convert viruses captured in the air into a liquid sample, and includes various injection and collection devices for purifying the sample and preparing it for analysis.

The eluent injection device is a core component of the eluent device, which injects the eluent (PBS) onto the collection surface to suspend the captured virus particles in the liquid. This causes the virus to be converted to a liquid state, ensuring uniform distribution and high precision of the sample.

The deionized water injection unit and the ethanol injection unit are the main components that support the cleaning and disinfection process of the elution device. The deionized water is used to clean the collection surfaces and the interior of the system, and the ethanol is used as a disinfectant to prevent contamination within the device and minimize cross-contamination.

The waste collection unit safely collects and processes filtered large particles or used eluent, while the sample collection unit collects purified liquid samples and delivers them to the virus detection sensor. This design is configured to efficiently support waste processing while maintaining sample quality.

The embodiments described above are combinations of components and features of the present invention in a given form. Each component or feature should be considered optional unless explicitly stated otherwise. Each component or feature may be implemented in a form that is not combined with other components or features. In addition, it is also possible to form an embodiment of the present invention by combining some components and/or features. The order of operations described in the embodiments of the invention may be changed. Some components or features of one embodiment may be included in another embodiment, or may be replaced with corresponding components or features of another embodiment. It is obvious that claims that do not have an explicit citation relationship in the scope of the patent claims may be combined to form an embodiment or may be included as a new claim by post-application amendment.

It will be apparent to those skilled in the art that the present invention can be embodied in other forms without departing from the technical idea and essential characteristics of the present invention. Accordingly, the above embodiments should be considered in all respects as illustrative rather than restrictive. The scope of the present invention should be determined by a reasonable interpretation of the appended claims and all possible changes within the equivalent scope of the present invention.

1000: Liquid sample generator 1101: Cyclone
1102: Cyclone 1103: Cyclone
1104: Particle Sensor 1105: Particle Sensor
1106: Particle Sensor 1107: Particle Sensor
1200: Dissolution device 1201: Collection surface
1202: Solution injection device 1203: Deionized water injection device
1204: Ethanol injection device 1205: Filtering device
1206: Sample collection section 1207: Waste collection section
1208: Fluid control device 1300: Control device
1301: Transmitter/Receiver 1302: Memory
1303: Processor 1304: Input Device
1305: Output Device 2000: Virus Detection Sensor

Claims (10)

In a liquid sample generating device for airborne viruses,
A cyclone device for removing particles from the air to produce an aerosol sample;
A dissolution device for producing a liquid sample from the above aerosol sample;
An air exhaust device for exhausting the air that has passed through the elution device after being introduced through the cyclone device to the outside;
A control device connected to the above cyclone device, the above elution device, and the above air exhaust device,
The cyclone device comprises: one or more cyclones for filtering particles in the air; a plurality of particle sensors for detecting particle characteristics of particles introduced into the cyclone device and particles filtered by the one or more cyclones; wherein the plurality of particle sensors comprises (i) an inlet particle sensor at an inlet through which air is introduced into the cyclone device and (ii) one or more cyclone particle sensors inside each of the one or more cyclones, and when the one or more cyclones are a plurality of cyclones, the plurality of cyclones are connected in series, and the plurality of cyclones are configured to filter particles having different particle sizes, respectively.
The above elution device comprises: a collection surface, which is a space where the aerosol sample is collected and an elution solution, which is phosphate buffered saline (PBS), is injected; an elution solution injection device for injecting the elution solution into the collection surface; a deionized water injection device for injecting deionized water (DI water) for diluting the elution solution into the collection surface to adjust the concentration of the elution solution injected into the collection surface; an ethanol injection device for injecting ethanol (EtOH) into the collection surface for disinfecting the collection surface; a filtering device for filtering particles larger than a predetermined size from the elution solution or a mixture obtained by eluting the aerosol sample with the elution solution and the deionized water; a sample collection unit for collecting the liquid sample that has passed through the filtering unit among the mixture; a waste collection unit for collecting waste filtered by the filtering unit among the mixture; a buffer solution injection unit for mixing a buffer solution, which is a lysis buffer, with the liquid sample; A fluid control device for controlling the flow of the above-mentioned eluent, the deionized water, the ethanol, the mixed solution, the liquid sample, and the buffer solution;
The air exhaust device comprises: an air purification filter device for purifying the air that has passed through the elution device after being introduced through the cyclone device; and an air outlet for discharging the air that has passed through the air purification filter to the outside.
The control device comprises: a transceiver; a memory; an input device; an output device; a processor functionally connected to the cyclone device, the elution device, the air discharge device, the transceiver, the memory, the input device and the output device;
Liquid sample generation device.
In the first paragraph,
The above processor:
The ratio and amount of the eluent and the deionized water injected into the collection surface are determined according to the particle characteristics detected by the plurality of particle sensors;
Based on the above-determined ratio and amount, the eluent, or the eluent and the deionized water are configured to be injected into the collecting surface by the eluent injection device and the deionized water injection device.
Liquid sample generation device.
In the second paragraph,
The above particle characteristics include one or more of particle size, particle concentration, particle mass, particle number, and particle capture efficiency,
The above particle concentration is the number of particles per unit volume,
The above particle mass is the total mass of particles captured by the one or more cyclones,
The above particle number is the total number of particles captured by the one or more cyclones,
The above particle collection efficiency is the ratio of the number of particles captured by one or more cyclones to the total number of particles entering the plurality of cyclones.
Liquid sample generation device.
In the third paragraph,
Based on the above particle characteristics, the processor:
If the number of particles larger than a standard size among the particles captured by the one or more cyclones is greater than the number of particles smaller than the standard size, the ratio of the eluent is increased and the ratio of the deionized water is decreased;
If the particle concentration of the particles captured by the one or more cyclones is higher than the reference concentration, the amount of the eluent is increased, the ratio of the eluent is increased, and the ratio of the deionized water is decreased;
If the total mass of particles captured by the one or more cyclones is higher than the reference mass, the amount of the eluent is increased, the ratio of the eluent is increased, and the ratio of the deionized water is decreased;
If the total number of particles captured by the above one or more cyclones is higher than the reference number, the amount of the eluent is increased, the ratio of the eluent is increased, and the ratio of the deionized water is decreased; or,
If the particle collection efficiency is higher than the reference efficiency, the amount of the eluent is increased, the ratio of the eluent is increased, and the ratio of the deionized water is further configured to be lowered.
Liquid sample generation device.
In the first paragraph,
The above filtering device is composed of one of a rotating liquid impactor or a membrane filter, or a combination of the rotating liquid impactor and the membrane filter,
The above rotating liquid impactor is configured to rotate the mixture so that large particles are captured in the liquid and small particles are discharged according to the size of the particles.
The above membrane filter is configured to capture large particles through the pores of the membrane filter and discharge small particles.
When the filtering device is composed of a combination of the rotating liquid impactor and the membrane filter, the filtering device is configured so that the rotating liquid impactor filters particles that are relatively larger than the membrane filter and then the membrane filter filters particles that are relatively smaller than the rotating liquid impactor.
Liquid sample generation device.
In the first paragraph,
The above mixture is composed of a solid component and a liquid component,
The above solid composition comprises sample particles smaller than a reference size that pass through the filtering device and are sent to the sample collection unit, and waste particles larger than the reference size that are filtered by the filtering device and sent to the waste collection unit.
The above liquid composition comprises the above eluent injected into the above collecting surface, or the above eluent and the above deionized water,
The above liquid composition is distributed through the fluid distribution valve by the fluid control device to the sample collection unit and the waste collection unit based on the set distribution ratio.
Liquid sample generation device.
In Article 6,
The above distribution ratio is based on the purity of the liquid sample or the efficient use of the eluent.
When the above distribution ratio is based on the purity of the liquid sample, the distribution ratio is set so that the ratio at which the liquid is distributed to the sample collection unit is higher than the reference sample liquid ratio and the ratio at which the liquid is distributed to the waste collection unit is lower than the reference waste liquid ratio.
If the above distribution ratio is based on efficient use of the above solvent, the distribution ratio is set so that the ratio of the liquid distributed to the sample collection unit is lower than the reference sample liquid ratio and the ratio of the liquid distributed to the waste collection unit is higher than the reference waste liquid ratio.
Liquid sample generation device.
In Article 6,
The above processor:
After the distribution of the above mixture to the sample collection unit and the waste collection unit is completed, the collection surface is washed by injecting the deionized water into the collection surface by the deionized water injection device;
After the collection surface is washed, the collection surface is further configured to be disinfected by injecting the ethanol into the collection surface by the ethanol injection device.
Liquid sample generation device.
In Article 6,
The above processor:
After the distribution of the above mixture to the sample collection unit and the waste collection unit is completed, the set amount of the provided sample among the liquid samples in the sample collection unit is further configured to be discharged to the virus detection sensor outside the liquid sample generation device by the fluid control device.
Liquid sample generation device.
In Article 9,
The above sample collection unit includes a liquid particle sensor,
The above liquid particle sensor is configured to detect at least one of the number or concentration of the sample particles in the liquid sample within the sample collection unit,
The concentration of said particles is based on the volume of said liquid sample within said sample collection unit and the number of said sample particles,
The volume of said liquid sample is based on the volume dispensed into said sample collection portion of said liquid composition,
The amount of the provided sample is determined by the processor based on the concentration of the sample particles,
Based on whether the concentration of the sample particles is within a set reference sample concentration range, higher than the reference sample concentration range, or lower than the reference sample concentration range, the provided sample amount is set to the reference sample amount, higher than the reference sample amount, or lower than the reference sample amount,
The extent to which the provided sample amount is set higher or lower than the reference sample amount is based on the ratio of the concentration of the sample particles to the median value of the reference sample concentration range.
Liquid sample generation device.

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