KR102685911B1 - Sensor cartridge for diagnostics and diagnostic method using the same - Google Patents

Abstract

The present invention relates to a diagnostic sensor cartridge for sensing a sample for diagnosis and a diagnostic method using the same.
A sensor cartridge for diagnosis is provided with an inlet through which a sample, a buffer solution, or water is injected, and the inlet has an upper part where a sensor for sensing a target material is provided, a distribution channel provided at the bottom of the upper part, and connected to the inlet. It is provided with a middle plate through which the sample or the buffer solution or the water injected through the injection port through the distribution channel flows, and a lower plate provided at the bottom of the middle plate and provided with a discharge chamber connected to the distribution channel. Includes.

Description

Sensor cartridge for diagnosis and diagnosis method using the same {SENSOR CARTRIDGE FOR DIAGNOSTICS AND DIAGNOSTIC METHOD USING THE SAME}

The present invention relates to a diagnostic sensor cartridge for sensing a sample for diagnosis and a diagnostic method using the same.

A sensor cartridge for diagnosis detects a target substance by roughly sensing a sample that has been pre-processed through a cartridge for sample preparation for diagnosis through a sensor.

In addition, in a sensor cartridge for diagnosis, the processes from sample injection to sensing, washing, and channel cleaning must be performed sequentially in an appropriate order, and an inlet, sensor, channel, outlet, etc. must be provided to perform the above processes.

Therefore, there is a need to develop a sensor cartridge for diagnosis that can efficiently perform the above processes and increase diagnostic accuracy and throughput.

The matters described as background technology above are only for the purpose of improving understanding of the background of the present invention, and should not be taken as recognition that they correspond to prior art already known to those skilled in the art.

KR 10-2019-0179960 A

The present invention was proposed to solve this problem, in which a pretreated sample is injected, a structure for accommodating a sensor chip is provided, fluid flows at the bottom of the sensor, and the positive and negative pressures are lowered in the chamber to form a channel. The purpose is to provide a sensor cartridge for diagnosis that can control fluid flow and a diagnosis method using the same.

A sensor cartridge for diagnosis according to the present invention to achieve the above object includes an upper plate provided with an inlet through which a sample, a buffer solution, or water is injected, and a sensor for sensing a target material is provided in the inlet; A middle plate portion provided at the bottom of the upper plate portion and provided with a distribution channel connected to the injection port, through which the sample or the buffer solution or the water injected through the injection port is distributed; and a lower plate portion provided at the bottom of the middle plate portion and provided with a discharge chamber connected to the distribution channel.

Positive or negative pressure is applied to the discharge chamber connected to the distribution channel, thereby allowing the sample, buffer solution, or water injected through the inlet to flow.

The sensor can incubate the target material present in the sample or the buffer solution and detect the target material fixed on the surface.

An injection channel connected to the injection port is provided at the top of the middle plate, and the injection channel may be formed as a groove formed along the upper surface of the middle plate.

The upper plate portion is provided with a connection channel at a point spaced apart from the injection port, and both ends of the connection channel are coupled to the injection channel and the distribution channel, respectively, thereby connecting the injection channel and the distribution channel.

When the sample, the buffer solution, or the water flows into the distribution channel, the sample, the buffer solution, or the water injected through the injection port rises through the injection channel and falls through the connection channel, It may flow into the above distribution channels.

The distribution channel is provided at the bottom of the middle plate and may be composed of a groove formed to repeat extension and bending along the lower surface of the middle plate.

The sample, buffer solution or water distributed in the distribution channel can clean the sensor by flowing back and forth between one side and the other side of the middle plate along the groove.

The upper plate portion is provided with a discharge channel at a point spaced apart from the inlet, and both ends of the discharge channel are coupled to discharge holes formed in the distribution channel and the discharge chamber, respectively, so that the distribution channel and the discharge chamber can be connected. .

When the sample, the buffer solution, or the water flows into the discharge chamber, the sample, the buffer solution, or the water rises through the distribution channel, descends through the discharge channel, and passes through the discharge hole. It may flow into the discharge chamber.

A plurality of first coupling grooves spaced apart along an edge are formed on the upper plate portion, and a plurality of coupling protrusions are formed on the lower plate portion at positions corresponding to the plurality of first coupling grooves. The upper plate portion and the lower plate portion can be coupled by being inserted into the plurality of first coupling grooves.

A plurality of second coupling grooves spaced apart along an edge are formed in the middle plate portion, and a plurality of coupling protrusions are formed in the lower plate portion at positions corresponding to the plurality of second coupling grooves, and the plurality of coupling protrusions are formed at positions corresponding to the plurality of second coupling grooves. By being inserted into the plurality of second coupling grooves, the middle plate portion and the lower plate portion can be coupled.

A diagnostic method using a sensor cartridge for diagnosis according to the present invention to achieve the above object includes the steps of injecting a sample, buffer solution, or water through an injection port; Incubating the sample or the target material in the buffer solution on the sensor provided in the injection port; cleaning the sensor by distributing the sample, the buffer solution, or the water to a distribution channel connected to the injection port; Discharging the sample, the buffer solution, or the water into a discharge chamber connected to the distribution channel; and detecting the target material fixed on the sensor.

In the step of cleaning the sensor, positive or negative pressure may be applied to the discharge chamber to allow the sample, buffer solution, or water to flow back and forth between the sensor and the distribution channel.

In the step of detecting the target material, the fixed target material can be detected by exchanging optical, electrical, or electrochemical signals with the sensor.

According to the sensor cartridge for diagnosis of the present invention and the diagnostic method using the same, a pretreated sample is injected, a structure for accommodating a sensor chip is provided, fluid flows at the bottom of the sensor, and positive and negative pressures are reduced in the chamber. By doing this, the fluid flow in the channel is controlled, and through this, sensing of pre-processed samples for diagnosis can be performed quickly and accurately.

1 is a diagram showing the overall shape of a sensor cartridge for diagnosis according to an embodiment of the present invention.
Figure 2 is a perspective view showing the upper part of a sensor cartridge for diagnosis according to an embodiment of the present invention.
Figure 3 is a plan view showing the upper part of a sensor cartridge for diagnosis according to an embodiment of the present invention.
Figure 4 is a bottom view showing the upper plate of a sensor cartridge for diagnosis according to an embodiment of the present invention.
Figure 5 is a perspective view showing the middle plate of a sensor cartridge for diagnosis according to an embodiment of the present invention.
Figure 6 is a plan view showing the middle plate of a sensor cartridge for diagnosis according to an embodiment of the present invention.
Figure 7 is a bottom view showing the middle plate of a sensor cartridge for diagnosis according to an embodiment of the present invention.
Figure 8 is a cross-sectional view of the upper plate and middle plate of the sensor cartridge for diagnosis according to an embodiment of the present invention in a combined state.
Figure 9 is a perspective view showing the lower plate of a sensor cartridge for diagnosis according to an embodiment of the present invention.
Figure 10 is a plan view showing the lower plate of a sensor cartridge for diagnosis according to an embodiment of the present invention.
Figure 11 is a bottom view showing the lower plate of a sensor cartridge for diagnosis according to an embodiment of the present invention.
Figure 12 is a flowchart of a diagnostic method using a sensor cartridge for diagnosis according to an embodiment of the present invention.

The embodiments described herein may be modified in various ways. Specific embodiments may be depicted in the drawings and described in detail in the detailed description. However, the specific embodiments disclosed in the attached drawings are only intended to facilitate understanding of the various embodiments. Accordingly, the technical idea is not limited to the specific embodiments disclosed in the attached drawings, and should be understood to include all equivalents or substitutes included in the spirit and technical scope of the invention.

Terms containing ordinal numbers, such as first, second, etc., may be used to describe various components, but these components are not limited by the above-mentioned terms. The above-mentioned terms are used only for the purpose of distinguishing one component from another.

In this specification, terms such as “comprise” or “have” are intended to designate the presence of features, numbers, steps, operations, components, parts, or combinations thereof described in the specification, but are not intended to indicate the presence of one or more other features. It should be understood that it does not exclude in advance the possibility of the existence or addition of elements, numbers, steps, operations, components, parts, or combinations thereof. When a component is said to be "connected" or "connected" to another component, it is understood that it may be directly connected to or connected to the other component, but that other components may exist in between. It should be. On the other hand, when it is mentioned that a component is “directly connected” or “directly connected” to another component, it should be understood that there are no other components in between.

Meanwhile, a “module” or “unit” for a component used in this specification performs at least one function or operation. And, the “module” or “unit” may perform a function or operation by hardware, software, or a combination of hardware and software. Additionally, a plurality of “modules” or a plurality of “units” excluding a “module” or “unit” that must be performed on specific hardware or performed on at least one processor may be integrated into at least one module. Singular expressions include plural expressions unless the context clearly dictates otherwise.

In addition, when describing the present invention, if it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the gist of the present invention, the detailed description thereof is abbreviated or omitted.

Hereinafter, various embodiments will be described in more detail with reference to the attached drawings.

1 is a diagram showing the overall shape of a sensor cartridge for diagnosis according to an embodiment of the present invention. Figure 2 is a perspective view showing the upper part of a sensor cartridge for diagnosis according to an embodiment of the present invention. Figure 3 is a plan view showing the upper part of a sensor cartridge for diagnosis according to an embodiment of the present invention. Figure 4 is a bottom view showing the upper plate of a sensor cartridge for diagnosis according to an embodiment of the present invention. Figure 5 is a perspective view showing the middle plate of a sensor cartridge for diagnosis according to an embodiment of the present invention. Figure 6 is a plan view showing the middle plate of a sensor cartridge for diagnosis according to an embodiment of the present invention. Figure 7 is a bottom view showing the middle plate of a sensor cartridge for diagnosis according to an embodiment of the present invention. Figure 8 is a cross-sectional view of the upper plate and middle plate of the sensor cartridge for diagnosis according to an embodiment of the present invention in a combined state. Figure 9 is a perspective view showing the lower plate of a sensor cartridge for diagnosis according to an embodiment of the present invention. Figure 10 is a plan view showing the lower plate of a sensor cartridge for diagnosis according to an embodiment of the present invention. Figure 11 is a bottom view showing the lower plate of a sensor cartridge for diagnosis according to an embodiment of the present invention. Figure 12 is a flowchart of a diagnostic method using a sensor cartridge for diagnosis according to an embodiment of the present invention.

A sensor cartridge for diagnosis according to an embodiment of the present invention is provided with an inlet 110 through which a sample, buffer solution, or water is injected, and a sensor 115 for sensing a target material is provided in the inlet 110, Upper part 100; A distribution channel 220 is provided at the bottom of the upper plate 100 and connected to the injection port 110, and the sample or the buffer is injected through the injection port 110 through the distribution channel 220. A middle plate portion 200 through which the solution or the water is distributed; and a lower plate portion 300 provided at the lower end of the middle plate portion 200 and provided with a discharge chamber 315 connected to the distribution channel 220.

In addition, in the sensor cartridge for diagnosis according to an embodiment of the present invention, the sensor 115 incubates the target material present in the sample or the buffer solution and detects the target material fixed on the surface. can do.

Specifically, a sample for which pretreatment has been completed is injected into the sensor cartridge for diagnosis according to an embodiment of the present invention, and the sample contains magnetic particles and a labeling material. It exists in a combined state, and the target material is detected through the sensor 115. Here, the target substance is a separated protein or nucleic acid, which is detected by the sensor 115, and molecular diagnosis, immunodiagnosis, etc. are performed using the sensor cartridge for diagnosis according to an embodiment of the present invention.

Meanwhile, in the sensor cartridge for diagnosis according to an embodiment of the present invention, positive or negative pressure is applied to the discharge chamber 315 connected to the distribution channel 220, so that the sample injected through the inlet 110 or the The buffer solution or the water can be allowed to flow.

Specifically, referring to FIG. 2, the injection port 110 is formed in a recessed shape on the upper surface of the upper plate 100, thereby guiding the injected fluid to collect and incubate at the upper part of the central sensor, As the inlet 110, the distribution channel 220, and the discharge chamber 315 are all connected, the fluid flow is controlled by controlling the pressure of the discharge chamber 315, thereby cleaning the sensor 115, etc. is to carry out.

For example, the sample (sample pretreated through a diagnostic cartridge), the buffer solution (PBS), or the water (ultrapure water) injected through the injection port 110 is subjected to negative pressure in the discharge chamber 315. In this case, it will flow from the injection port 110 side through the distribution channel 220 toward the discharge chamber 315, and when positive pressure is applied to the discharge chamber 315, the discharge chamber 315 side It will flow through the distribution channel 220 toward the injection port 110.

In addition, in the sensor cartridge for diagnosis according to an embodiment of the present invention, the sensor 115 is located in the inlet 110 provided in the upper plate 100, and the inlet 110 supports the sensor 115. A cross-shaped support structure (6 x 6 x 0.7 mm) is formed, and the sensor 115 is seated on the support structure to sense the target material in the sample.

Meanwhile, in the sensor cartridge for diagnosis according to an embodiment of the present invention, an injection channel 210 connected to the injection port 110 is provided at the top of the middle plate 200, and the injection channel 210 is connected to the injection port 110. It may be composed of a groove formed along the upper surface of the middle plate portion 200.

In addition, in the sensor cartridge for diagnosis according to an embodiment of the present invention, the upper plate portion 100 is provided with a connection channel 120 at a point spaced apart from the injection port 110, and the connection channel 120 has two The end portions are coupled to the injection channel 210 and the distribution channel 220, respectively, so that the injection channel 210 and the distribution channel 220 can be connected.

Specifically, referring to FIG. 2, the connection channel 120 is a groove formed along the upper surface of the upper plate portion 100, and both ends penetrate the lower side of the upper plate portion 100 to penetrate the middle plate portion 200. It is connected to the injection channel 210 and the distribution channel 220. In addition, a laminating film is attached to the upper surface of the upper plate 100, so that the groove formed along the upper surface of the upper plate 100 is closed by the laminating film, thereby forming the connection channel 120. That is, by forming grooves in the upper plate portion 100 and the middle plate portion 200 and then forming channels through laminating, the manufacturing process can be simplified and manufacturing costs can be reduced.

Therefore, in the sensor cartridge for diagnosis according to an embodiment of the present invention, when the sample, the buffer solution, or the water flows into the distribution channel 220, the sample injected through the inlet 110 or The buffer solution or the water may rise through the injection channel 210, fall through the connection channel 120, and flow into the distribution channel 220.

In this way, the structure is configured so that the fluid repeats its rise and fall through the connection channel 120 and the discharge channel 130 of the upper plate 100, and as negative pressure is applied to the discharge chamber 315, the injection hole From (110), this is to prevent the siphon effect in which all the fluid in the channel escapes temporarily due to negative pressure. Ultimately, this structure allows the flow of fluid to be controlled more precisely at the desired flow rate.

In addition, referring to FIG. 4, the lower surface of the upper plate part 100 is provided with O-rings at the inlet and outlet portions of the inlet 110, the connection channel 120, and the discharge channel 130 to form the middle plate part 200 and the middle plate part 200. It can prevent fluid from leaking from each connected part.

Meanwhile, in the sensor cartridge for diagnosis according to an embodiment of the present invention, the distribution channel 220 is provided at the bottom of the middle plate 200, and extends and bends repeatedly along the lower surface of the middle plate 200. It may be composed of a groove formed to do so.

In this case as well, a laminating film is attached to the lower surface of the middle plate 200, and the distribution channel 220 is formed by the laminating film closing the groove formed on the lower surface of the middle plate 200. As a result, the middle plate portion 200, like the upper plate portion 100, can simplify the manufacturing process and reduce manufacturing costs.

Meanwhile, in the sensor cartridge for diagnosis according to an embodiment of the present invention, the sample, the buffer solution, or the water distributed in the distribution channel 220 is distributed along the groove on one side and the other side of the middle plate portion 200. The sensor 115 can be cleaned by flowing back and forth.

That is, by flowing the sample, the buffer solution, or the water back and forth between the sensor and the distribution channel, impurities remaining on the sensor 115 and substances that have non-specifically reacted are washed.

In addition, as the distribution channel 220 is formed as a flow path with a large cross-sectional area along the surface of the middle plate 200, when the sample is filled in the distribution channel 220, the middle plate 200 The accuracy and sensing speed of sensing by the provided sensor 115 can be improved.

Meanwhile, in the sensor cartridge for diagnosis according to an embodiment of the present invention, the upper plate portion 100 is provided with a discharge channel 130 at a point spaced apart from the injection port 110, and the discharge channel 130 is located at both ends. By being coupled to the discharge hole 310 formed in the distribution channel 220 and the discharge chamber 315, respectively, the distribution channel 220 and the discharge chamber 315 can be connected.

In the case of the discharge channel 130, since it has a similar principle and effect as the connection channel 120, in the sensor cartridge for diagnosis according to an embodiment of the present invention, the sample or the When the buffer solution or the water flows in, the sample, the buffer solution, or the water rises through the distribution channel 220, descends through the discharge channel 130, and passes through the discharge hole 310. It may flow into the discharge chamber 315.

Meanwhile, in the sensor cartridge for diagnosis according to an embodiment of the present invention, a plurality of first coupling grooves 140 spaced apart along the edge are formed in the upper plate portion 100, and the plurality of first coupling grooves 140 are formed in the lower plate portion 300. A plurality of coupling protrusions 320 are formed at positions corresponding to the first coupling grooves 140, and the plurality of coupling protrusions 320 are inserted into the plurality of first coupling grooves 140 to form the upper plate portion 100. ) and the lower plate portion 300 can be combined.

In addition, in the sensor cartridge for diagnosis according to an embodiment of the present invention, a plurality of second coupling grooves 230 spaced apart along the edge are formed in the middle plate portion 200, and the lower plate portion 300 is formed in the middle plate portion 200. A plurality of coupling protrusions 320 are formed at positions corresponding to the plurality of second coupling grooves 230, and the plurality of coupling protrusions 320 are inserted into the plurality of second coupling grooves 230 to form the middle plate portion. (200) and the lower plate portion 300 can be combined.

Specifically, referring to FIG. 9, a total of eight coupling protrusions 320 are formed along the edge of the lower plate portion 300, and the plurality of coupling protrusions 320 are connected to the second coupling groove 230 and the It is inserted and coupled into the first coupling groove 140. In addition, the plurality of coupling protrusions 320 are formed in an 'ㄱ' shape at the top, and are rigidly coupled to the jaw of the first coupling groove 140, thereby forming the upper plate portion 100, the middle plate portion 200, and the upper plate portion 100. The coupled state of the lower plate portion 300 can be firmly maintained.

A diagnostic method using a sensor cartridge for diagnosis according to an embodiment of the present invention includes the steps of injecting a sample, buffer solution, or water through the injection port 110 (S100); Incubating the sample or the target material in the buffer solution on the sensor 115 provided in the injection port 110 (S200); Cleaning the sensor 115 by distributing the sample, the buffer solution, or the water through the distribution channel 220 connected to the injection port 110 (S300); Discharging the sample, the buffer solution, or the water into the discharge chamber 315 connected to the distribution channel 220 (S400); and detecting the target material fixed on the sensor 115 (S500).

In addition, in the step (S300) of cleaning the sensor 115 in the diagnostic method using a sensor cartridge for diagnosis according to an embodiment of the present invention, positive or negative pressure is applied to the discharge chamber 315 to determine whether the sample or The buffer solution or the water may flow back and forth between the sensor 115 and the distribution channel 220.

Meanwhile, in the step (S500) of detecting the target material in the diagnostic method using a sensor cartridge for diagnosis according to an embodiment of the present invention, optical, electrical, or electrochemical signals are exchanged with the sensor 115. This makes it possible to detect fixed target materials.

Specifically, in the sample sensing step (S300), the surface of the sensor and the sample (50 ul) injected by the pipette are reacted at a specific temperature for about 1 hour, and then the discharge chamber 315 is Create a negative pressure and discharge the sample for about 20 seconds.

Afterwards, the tip of the pipette is replaced, and a cleaning operation is performed with the pipette with the replaced tip. During the washing operation, washing buffer (PBS, 200ul) and ultrapure water (DI water, 200ul) are injected from the buffer module using the pipette (20 seconds) into the inlet 110, and the discharge chamber 315 is maintained at positive and negative pressure. By repeated application, the injection port 110, the connection channel 120, the discharge channel 130, and the distribution channel 220 are cleaned (25 minutes), and when the cleaning is completed, the discharge chamber 315 is subjected to negative pressure. is applied to discharge the washing buffer and the ultrapure water into the discharge chamber 315 (10 seconds).

Although embodiments of the present invention have been described in more detail with reference to the accompanying drawings, the present invention is not necessarily limited to these embodiments, and various modifications may be made without departing from the technical spirit of the present invention. . Accordingly, the embodiments disclosed in the present invention are not intended to limit the technical idea of the present invention but are for illustrative purposes, and the scope of the technical idea of the present invention is not limited by these embodiments. Therefore, the embodiments described above should be understood in all respects as illustrative and not restrictive. The scope of protection of the present invention should be interpreted in accordance with the claims below, and all technical ideas within the equivalent scope should be construed as being included in the scope of rights of the present invention.

100: upper plate 110: injection port
115: sensor 120: connection channel
130: discharge channel 140: first coupling groove
200: middle plate 210: injection channel
220: Distribution channel 230: Second combining home
300: lower plate 310: discharge hole
315: discharge chamber 320: coupling protrusion

Claims (15)

An upper plate portion provided with an injection port through which a sample, a buffer solution, or water is injected, and a sensor for sensing a target material is provided at the injection port;
A middle plate portion provided at the bottom of the upper plate portion and provided with a distribution channel connected to the inlet, through which the sample or the buffer solution or the water injected through the inlet is distributed; and
A lower plate portion provided at the bottom of the middle plate portion and provided with a discharge chamber connected to the distribution channel,
The sensor is a sensor cartridge for diagnosis, characterized in that the target material present in the sample or the buffer solution is incubated and the target material fixed on the surface is detected. In claim 1,
A sensor cartridge for diagnosis, wherein positive pressure or negative pressure is applied to the discharge chamber connected to the distribution channel to cause the sample, buffer solution, or water injected through the injection port to flow. delete In claim 1,
A sensor cartridge for diagnosis, characterized in that an injection channel connected to the injection port is provided at the top of the middle plate, and the injection channel is composed of a groove formed along the upper surface of the middle plate. In claim 4,
The upper plate is provided with a connection channel at a point spaced apart from the injection port, and both ends of the connection channel are coupled to the injection channel and the distribution channel, respectively, thereby connecting the injection channel and the distribution channel. sensor cartridge for In claim 5,
When the sample, the buffer solution, or the water flows into the distribution channel, the sample, the buffer solution, or the water injected through the injection port rises through the injection channel and falls through the connection channel, A sensor cartridge for diagnosis, characterized in that it flows into the distribution channel. In claim 1,
The distribution channel is provided at the bottom of the middle plate and consists of a groove formed to repeat extension and bending along the lower surface of the middle plate. In claim 7,
A sensor cartridge for diagnosis, wherein the sample, the buffer solution, or the water distributed in the distribution channel cleans the sensor by flowing back and forth between one side and the other side of the middle plate along the groove. In claim 1,
The upper plate portion is provided with a discharge channel at a point spaced apart from the inlet, and both ends of the discharge channel are coupled to discharge holes formed in the distribution channel and the discharge chamber, respectively, thereby connecting the distribution channel and the discharge chamber. Featured sensor cartridge for diagnostics. In claim 9,
When the sample, the buffer solution, or the water flows into the discharge chamber, the sample, the buffer solution, or the water rises through the distribution channel, descends through the discharge channel, and passes through the discharge hole. A sensor cartridge for diagnosis, characterized in that it flows into the discharge chamber. In claim 1,
A plurality of first coupling grooves spaced apart along an edge are formed on the upper plate portion, and a plurality of coupling protrusions are formed on the lower plate portion at positions corresponding to the plurality of first coupling grooves. A sensor cartridge for diagnosis, characterized in that the upper plate portion and the lower plate portion are coupled by being inserted into a plurality of first coupling grooves. In claim 1,
A plurality of second coupling grooves spaced apart along an edge are formed in the middle plate portion, and a plurality of coupling protrusions are formed in the lower plate portion at positions corresponding to the plurality of second coupling grooves, and the plurality of coupling protrusions are formed at positions corresponding to the plurality of second coupling grooves. A sensor cartridge for diagnosis, characterized in that the middle plate portion and the lower plate portion are coupled by being inserted into the plurality of second coupling grooves. Injecting a sample, buffer solution, or water through an injection port;
Incubating the sample or the target material in the buffer solution on the sensor provided in the injection port;
cleaning the sensor by distributing the sample, the buffer solution, or the water to a distribution channel connected to the injection port;
Discharging the sample, the buffer solution, or the water into a discharge chamber connected to the distribution channel; and
A diagnostic method using a sensor cartridge for diagnosis, comprising: detecting the target material fixed on the sensor. In claim 13,
In the step of cleaning the sensor, a sensor cartridge for diagnosis is used to cause the sample, the buffer solution, or the water to flow back and forth between the sensor and the distribution channel by applying positive or negative pressure to the discharge chamber. Diagnosis method used. In claim 13,
In the step of detecting the target material, a diagnostic method using a sensor cartridge for diagnosis, characterized in that the fixed target material is detected by exchanging optical, electrical, or electrochemical signals with the sensor.

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