KR102481125B1 - A composition for skin anti-pollution containing a daughter-in-law navel extract - Google Patents

Abstract

The present invention relates to a composition for skin anti-pollution containing a daughter-in-law's navel extract, which contains a natural extract harmless to the human body and can effectively inhibit skin cell death caused by chemicals while alleviating skin irritation. In addition, skin moisturizing and skin regeneration effects can be improved.

Description

Composition for skin anti-pollution containing daughter-in-law's navel extract

The present invention relates to a composition for skin anti-pollution comprising a daughter-in-law's navel extract. More specifically, it relates to a composition containing a daughter-in-law's navel extract that is excellent in inhibiting the death of skin cells caused by chemicals and has excellent skin moisturizing and skin regeneration effects.

Recently, as the quality of life is improved due to the extension of average sleep due to the development of medical technology, the desire for a healthy life increases, and at the same time, interest in skin care is also increasing.

The skin is a body part directly exposed to the external environment, and it not only serves as a protective film to protect important organs of our body, but also controls moisture evaporation and protects the body from external infections. However, no matter how much the skin blocks virus penetration from the outside, stress from the outside, such as excessive ultraviolet rays and a polluted environment, causes skin irritation and eventually leads to skin aging.

As we enter modern society, as environmental pollution gets worse and the concentration of pollutants around the living radius increases, the problem of skin protection from pollutants has emerged as a social problem. Accordingly, various functional cosmetics are being developed, and in particular, investment and research on skin protection cosmetics containing anti-pollution functions are being actively conducted.

Examples of such pollutants include formaldehyde, nitrogen oxides, and benzopyrene, which enter the body and accumulate to cause various diseases.

Specifically, the pollutants cause direct inflammation or necrosis when they come into contact with the skin at high concentrations, and even if they are present in residual amounts in the air, they cause continuous stress on the skin, thus causing skin aging.

Compared to the effects of the above pollutants on the skin, the public's awareness of the risks is still low, but as issues such as fine dust have recently emerged, the risks are gradually attracting attention, and people who are gradually aware of the dangers of pollutants are increasing. The number is growing.

Accordingly, development of cosmetic compositions, detergents, and external preparations having an anti-pollution effect to prevent the above environmental contaminants from being attached to the surface of the skin or penetrating into the body through the skin is being actively developed. However, most of the currently developed products simply remove contaminants attached to or penetrated into the skin, but simply remove contaminants from the skin, and do not fundamentally protect the skin from environmental contaminants. There was a problem that the ability to protect the skin from was insignificant.

In particular, since it contains chemicals as main components, there is a problem of acting as a cause of skin troubles and skin diseases. Accordingly, it is necessary to develop a composition using natural substances that is mild to the skin and has an excellent anti-pollution effect.

On the other hand, as a natural material, Korean Patent Registration No. 10-1571323 uses an herbal composite extract extracted by mixing peppermint, chamomile, rosemary, lemongrass, wild chrysanthemum, tea tree and lavender in equal weight ratios, A cosmetic composition for skin improvement having a lubrication effect is disclosed, and Korean Patent Publication No. 10-2017-0053388 discloses a cosmetic composition having an anti-pollution effect by containing turmeric, apple, and grape extracts. , In the case of the above patent, there was a problem that the anti-pollution effect was insignificant.

On the other hand, as examined above, the skin is in direct contact with the external environment and is in charge of protecting our body from various harmful factors, but it is gradually damaged by the increase in age, the influence of ultraviolet rays, external pollutants and stress, resulting in cell protection and Proliferative ability is lowered, thereby accelerating skin aging.

Specifically, this skin aging phenomenon is mainly due to the cleavage and low-molecularization of hyaluronic acid, the sluggish production of collagen in fibroblasts, etc., which reduces the ability of the stratum corneum and dermis to retain moisture, and elastine fibrous tissue It is manifested by a decrease in skin elasticity due to weakening of the skin.

In general, the moisture content of the skin is about 70% in the dermal layer, but decreases toward the epidermal layer, and becomes about 10 to 30% in the keratinocyte layer. The water supplied from the dermal layer moves to the top of the stratum corneum mainly by passive diffusion and is finally discharged to the outside. This is called transepidermal water loss (TEWL), and it is known that this transepidermal water loss is maintained at an appropriate level by sebum components and epidermal lipids, which are lipid film components of the keratinocyte layer.

In addition, it is known that a substance having a hydrophilic water-retaining ability, called Natural Moisturizing Factor (NMF), exists in the keratinocyte layer, and plays an important role in moisturizing the skin. When the moisture of the keratinocyte layer is maintained at about 10 to 30%, the skin is smooth and soft, and the body protective function is normally exhibited. However, when the moisture content of the keratinocyte layer is less than 10%, the skin becomes rough and loses the body's protective function, resulting in aging.

For example, in the case of dry skin, the cohesion of keratinocytes is weakened, resulting in a scale phenomenon in which keratinocytes peel off white like scales from the skin surface. This is because it is smaller than normal skin. In addition, even if the skin is healthy, moisture deficiency may occur due to harsh external environments, such as wind, cold weather, sunlight, face washing and shaving, and the skin condition may deteriorate. Therefore, it is most important to prevent skin aging to properly maintain the moisture content of the keratinocyte layer of the skin.

To this end, cosmetics have been used by adding components similar to sebum, NMF components, or moisturizers such as polyols.

For example, glycerin and sorbitol, which have three or more hydroxyl groups (-OH groups), have been added as water-soluble polyols, and while these have the advantage of exhibiting excellent moisturizing power, they are sticky and feel uncomfortable when used, and can cause skin irritation depending on the relative humidity. I have a problem with taking my moisture away. In addition, side effects on the skin may occur when propylene glycol and 1,3-butylene glycol having two hydroxyl groups (-OH groups) are added. Additives such as other natural moisturizing factors, such as sodium pyrrolidone carboxylate (PCA-Na) and sodium lactate, have strong electrolytic properties and impair the emulsification stability of cosmetics. It is known that there is, but there is a limit to its moisturizing ability.

Accordingly, the applicant of the present invention has solved the above problems and developed a composition containing daughter-in-law's navel extract, which is a natural extract, as a composition having excellent skin protection effects from chemicals, as well as excellent skin moisturizing and skin regeneration effects.

KR 10-1571323 B1 KR 10-2017-0053388 A

An object of the present invention is to provide a composition for skin anti-pollution containing a daughter-in-law's belly button extract, which contains a daughter-in-law's belly button extract, which is a natural extract without skin irritation, to effectively suppress the death of skin cells caused by chemicals, thereby reducing the skin In addition to protecting, it is to provide a composition capable of improving skin moisturizing and skin regeneration effects.

Another object of the present invention is to provide a cosmetic composition for skin anti-pollution comprising a daughter-in-law's navel extract as an active ingredient.

Another object of the present invention is to provide a lotion containing a daughter-in-law's navel extract as an active ingredient.

In order to achieve the above object, the skin anti-pollution composition comprising the daughter-in-law's belly extract according to an embodiment of the present invention contains the daughter-in-law's belly button extract as an active ingredient, the effect of inhibiting the death of skin cells by chemicals is excellent

The chemical is selected from the group consisting of polycyclic aromatic hydrocarbons, aldehydes and nitrogen oxides.

The composition has excellent skin moisturizing and skin regeneration effects.

The composition further comprises a first natural extract selected from the group consisting of a dume leek extract, a silage tree extract, and a mixture thereof.

The composition further includes other additives, and the other additives are excipients, disintegrants and lubricants.

In the present invention, the extract is extracted using an extraction solvent selected from the group consisting of water, alcohol having 1 to 4 carbon atoms, ether, chloroform, ethyl acetate, dimethyl sulfoxide, and mixtures thereof.

A cosmetic composition for skin anti-pollution according to another embodiment of the present invention includes the composition.

A lotion according to another embodiment of the present invention includes the above composition.

Hereinafter, the present invention will be described in more detail.

The term 'extract' used herein has a meaning commonly used in the art as a crude extract, but also includes fractions obtained by further fractionation of the extract in a broad sense. That is, the plant extract includes not only those obtained using the above-described extraction solvent, but also those obtained by additionally applying a purification process thereto. For example, a fraction obtained by passing the extract through an ultrafiltration membrane having a certain molecular weight cut-off value, separation by various chromatography (made for separation according to size, charge, hydrophobicity or affinity), etc. Fractions obtained through various purification methods are also included in the extract of the present invention.

The extract used in the present invention may be prepared in a powder state by additional processes such as distillation under reduced pressure and freeze drying or spray drying.

As used herein, the term "comprising as an active ingredient" refers to an amount sufficient to affect a desired biological effect, such as beneficial results, including but not limited to prevention, reduction, alleviation or elimination of signs or symptoms of a disease or disorder. , and this amount is referred to as the “effective amount”. Thus, the total amount of each active ingredient in the composition or method is sufficient to yield a significant individual benefit. Thus, the effective amount will depend on the circumstances in which it is being administered. An effective amount can be administered in one or more prophylactic or therapeutic administrations.

The skin anti-pollution composition containing the daughter-in-law's navel extract according to an embodiment of the present invention contains the daughter-in-law's navel extract as an active ingredient, and has an excellent effect of inhibiting the death of skin cells by chemicals.

Anti-pollution means 'to protect the body from various environmental harmful factors such as fine dust and prevent pollution', and anti-pollution products such as fine dust and sunscreen, KF94 certified masks, air purifiers, and wet mop vacuum cleaners are representative products. It is a product of lution.

On the other hand, the problem of environmental pollution has recently emerged due to adverse effects caused by rapid urbanization, and chemicals contained in various pollutants and fine dust act on the skin, causing various skin diseases. Specifically, when various chemicals act on the skin, it not only causes the death of skin cells, but also develops various skin diseases such as inflammation by inhibiting the proliferation of skin cells.

Throughout the specification of the present invention, "anti-pollution" means improving or preventing skin contamination, skin cell death, or inhibition of skin cell proliferation by chemicals.

On the other hand, the daughter-in-law navel (Persicaria perfoliata) is a kind of node grass whose origin is in Asia. Daughter-in-law's belly button has traditionally been used as an antidote, antipyretic, and diuretic, and has also been used for respiratory diseases such as cough and whooping cough. It is known that daughter-in-law's navel extract contains quercetin, caffeic acid, and the like as major components. The quercetin (quercetin) is a plant antioxidant and belongs to the flavonoid family among phytochemicals. Quercetin plays a role in regulating the immune system's response to external stressors through cell signaling pathways called kinases and phosphatases, two types of enzymes and membrane proteins required for proper cell function.

When the daughter-in-law's belly button extract is included in the composition, the death of skin cells caused by the action of various chemicals on the skin can be effectively inhibited by the action of the active ingredient contained in the daughter-in-law's belly button extract, It has the effect of improving skin moisturization and skin elasticity.

The chemical is selected from the group consisting of polycyclic aromatic hydrocarbons, aldehydes and nitrogen oxides.

Specifically, the polycyclic aromatic hydrocarbon may include benzopyrene, and the aldehydes may include formaldehyde.

In addition, the nitrogen oxides may include nitrogen oxides represented by the chemical formula NO _X , more specifically NO, NO ₂ , N ₂ O, N ₄ O, N ₂ O ₃ , N ₂ O ₄ , N ₂ O ₅ , N ₄ O ₆ or ionized products thereof.

The benzopyrene is produced due to incomplete combustion such as automobile exhaust gas or cigarette smoke, and is a toxic substance that causes cell damage through metabolic conversion to epoxide and reaction with DNA. Recently, as news that it causes atopic dermatitis has been reported, adverse effects on the skin have been revealed, and it is one of the dangerous substances that can cause skin deterioration due to problems such as cell mutation and cell damage.

The formaldehyde is a colorless gas with a strong irritating odor at room temperature and is also called methaneal. The formaldehyde is easily produced when materials containing carbon are incompletely burned, and is synthesized in the air when sunlight and oxygen are applied to hydrocarbons such as methane. The formaldehyde is one of the skin allergens, and may cause problems such as skin rash, skin necrosis, and dermatitis upon contact with the skin.

The nitrogen oxide is a compound composed of nitrogen and oxygen, and includes nitrogen monoxide, nitrogen dioxide, dinitrogen monoxide, dinitrogen trioxide, dinitrogen tetroxide, dinitrogen pentoxide, and the like, and ionized products thereof. In general, nitrogen gas is stable and it is difficult for the atoms constituting it to be split into two, but the bond can be broken when a very high temperature is applied. Often, high temperatures are formed inside the engine of a vehicle, so that the exhaust gas of the vehicle contains a large amount of nitrogen oxides.

The nitrogen oxide may include ammonium or a salt thereof. Nitric acid, another form of nitrogen oxide, reacts with water to form nitric acid and is also responsible for acid rain. The nitric acid combines with ammonium, an alkaline substance in the air, to produce ammonium nitrate, and the ammonium nitrate is a particulate matter in the air, which is secondary fine dust and adversely affects the human body. When the ammonium nitrate comes into contact with the human body, it causes inflammation in the eyes, nasal mucosa and skin.

Specifically, the chemicals may act on the skin and promote the death of skin cells, which causes skin aging.

On the other hand, the composition is excellent in skin moisturizing and skin regeneration effects.

Fundamentally, moisturizing of the skin allows keratinocytes to maintain a well-arranged structure due to intercellular lipids and serves as a passage through which physiologically necessary substances are absorbed to keep the skin moist and healthy. That is, the lamellar liquid crystal structure formed by intercellular lipids can prevent water loss to the outside and retain water in the body well, thus serving as a skin barrier. At this time, when the function as a skin barrier of cellular lipids is damaged, water loss is high and water content is insufficient, so that direct supply of water is required.

In addition, the keratinocytes of healthy people have a high concentration of Natural Mosituring Factors (NMF) to help retain moisture in the skin. However, despite these functions of the skin, the skin is exposed to harsh external environments, such as wind, cold weather, sunlight, and polluted air along with face washing, shaving, and indoor heating in winter, and the aging factor is added as we age. Since the skin easily loses moisture and becomes dry, the need for a skin moisturizer is increasing to prevent this. According to this need, various studies are being conducted to show the effect of skin moisturizing by increasing water retention in the stratum corneum. On the other hand, recently, it has been newly discovered that, in addition to simply increasing moisture in the stratum corneum, the differentiation process in which keratinocytes in the basal layer develop into keratinocytes in the stratum corneum must be carried out normally to play a role as a skin moisture barrier. Accordingly, studies are also being conducted to promote the differentiation of keratinocytes and ultimately improve the moisturizing power of the skin.

On the other hand, among the theories of skin aging, the most attention-grabbing theory is aging by active oxygen. About 95% or more of the oxygen ingested by the human body is reduced to water by combining with electrons generated in the metabolic process of cells, but some 2 to 3% of oxygen causes destruction of cells in the free radical process to absorb electrons through incomplete reduction. This is called active oxygen. Active oxygen is generated not only from various factors that threaten our health, such as smoking, stress, excessive exercise, skin disease, viral infection and ultraviolet rays, but also during the process of breathing and digestion, which are essential for maintaining life. Therefore, humans are constantly exposed to the threat of free radicals, and therefore, our bodies have various antioxidant mechanisms in response to damage caused by free radicals to protect cells in various ways.

In particular, active oxygen causes damage to skin cells. Excessive active oxygen destroys the body's antioxidant defense system, and as a result, cellular components such as proteins, lipids, and DNA are damaged, resulting in altered cell functions and ultimately accelerated skin aging. Also, free radicals damage connective tissue. Active oxygen not only directly destroys collagen by acting on collagen metabolism, but also causes aging of skin cells by causing collagen deficiency.

The daughter-in-law navel extract, which is an active ingredient included in the composition for skin anti-pollution of the present invention, is a natural extract harmless to the human body, and removes dead skin cells and other wastes by adsorbing harmful substances present on the skin without irritating the skin surface. This can prevent skin aging.

More specifically, the action of the active ingredient of the daughter-in-law's navel extract promotes the activity of fibroblasts, thereby improving the skin regeneration effect, as well as improving skin moisturizing power by strengthening the skin barrier and increasing the moisture content. .

On the other hand, the skin regeneration is not only skin regeneration, but also wrinkle improvement, skin elasticity improvement, skin aging prevention, skin moisturizing improvement, age spot removal, acne treatment and wound removal, and more preferably skin regeneration, wrinkle improvement, It means improving skin elasticity and preventing skin aging.

The composition further comprises a first natural extract selected from the group consisting of a dume leek extract, a silage tree extract, and a mixture thereof.

The dume leek (Allium senescens), like the commonly known leek, is a perennial monocotyledonous plant belonging to the Liliaceae family and is mainly distributed around Ulleungdo and northern regions. Scaly stems are egg-shaped ovals, and the outer skin is thin and membranous and has no fibers. In addition, Dume leek has thick leaves and rich flesh, so it contains a large amount of moisture and essential oils, and is classified as a plant with a warm nature in oriental medicine. It is known. Recently, as it has been found to contain a large amount of polyphenols known as antioxidants, it is expected that they will be able to exert excellent effects on anti-aging, which is the greatest concern of modern people.

In particular, dume leek contains a large amount of adenosine and saponin components, thereby preventing blood clots from being formed and clearing the blood, which is effective in preventing cerebrovascular disease and high blood pressure. In addition, the mucin component of dume leek helps digestion when ingested, protects the stomach wall, and detoxifies the body by discharging cholesterol. Allicin, a component that gives off the spicy scent of dume leek, has antibacterial and anti-inflammatory effects on the body, which helps with various inflammations such as gastritis, enteritis, and bronchitis, and has the effect of releasing poison accumulated in the liver. However, since it has a warm nature, a person with a high fever may feel a sense of heat if consumed, and excessive intake at once may cause abdominal pain and diarrhea.

The thread tree (Caesalpinia decapetala) is a vine-like deciduous shrub belonging to the dicotyledon rose order legume, and is also called a belt tree and a flesh tree. It grows in sunny places at the foot of mountains and is widely distributed in Korea, Japan, southern China, and India. The length is about 4m, and there are thorns bent all over, and the leaves are alternate, with twice pinnate compound leaves, and there are middle-aged bulges in the axils. Small leaves are 5 to 10 pairs, oval, and about 1 to 2 cm long. Fruits are legumes, flat elliptical, about 9cm long, about 2.7cm wide, and do not open. Silgeori tree is mainly used as a fruit that is effective in headache or muscle pain in the private sector.

Preferably, the composition may include 15 to 20 parts by weight of Dume leek extract and 15 to 20 parts by weight of Silgeoli tree extract, based on 100 parts by weight of daughter-in-law's navel extract, and in the case of the above parts by weight range, skin caused by chemicals Not only can the anti-pollution effect on the skin be improved by more effectively inhibiting cell death, but also the skin moisturizing and skin regeneration effects can be further increased.

More preferably, the composition may further include a second natural extract consisting of a mixture of a tea tree extract and a pheasant tree extract.

The sweet tree (Raphiolepis indica var. umbellata (Thunb.) Ohashi) is an evergreen shrub growing at the foot of a coastal mountain, reaching a height of 2 to 4 m and having twigs. Leaves grow alternately, but seem to grow at the ends of branches, are long oval or obovate, obtuse, and have dull sawtooth on the edge. Flowers are white and run in panicle in April to June. The fruit is a pome, round and black when ripe.

The fruit of the sweet tree has the effect of dilating blood vessels and improving blood flow, so it is used for heart diseases such as irregular heartbeat, high blood pressure, and chest pain.

The lesser pheasant tree (Viburnum erosum Thunb.) is a deciduous broad-leaved shrub belonging to the genus Indong and lesser pheasant, and is a tree commonly found in low mountains in the south of Gyeonggi-do. The height is 2 to 3m, and it is often planted as a park tree because of its beautiful white flowers in spring and red fruits in autumn. The elliptical leaves are characterized by running facing each other, and the hairs on the front and back give a soft feeling when touched. The stem is divided into several pieces and grows by forming a branch. Flowers bloom in April or May, forming a round shape with white bisexual flowers at the end of newly emerging branches. The small red bean-like fruits ripen red in September or October and are good food for birds. It grows in a forest with moderate sunlight, likes soil with good moisture and drainage, and is very cold-resistant and grows well in both sunny and shaded areas. On the other hand, the name Leolpheasant Tree seems to be related to pheasants, and it is assumed that it was called Leelpheasant Tree, which means that pheasants in the field have a favorite fruit, and then became Leorpheasant Tree. Young leaves can be eaten as herbs, and old leaves and stems are used as medicine for stomatitis or itching in oriental medicine.

When the composition further includes the second natural extract of the sweet tree extract and the pheasant tree extract, through the mixing action of the navel extract and the first natural extract, the death of skin cells caused by chemicals can be more effectively inhibited In addition, it is possible to regenerate the skin and further improve skin elasticity by enhancing the cellular activity of fibroblasts. In addition, it is possible to maximize the skin moisturizing effect by increasing the moisture content in the skin.

More preferably, with respect to 100 parts by weight of daughter-in-law's navel extract, 15 to 20 parts by weight of Dume leek extract, 15 to 20 parts by weight of Silgeoli tree extract, 5 to 10 parts by weight of Datonggeum tree extract, and 5 to 10 parts by weight of Chuckus japonica extract. can

In the case of the weight range, not only can the effect of inhibiting the death of the skin cells be maximized, but also skin moisturizing and skin regeneration effects can be further improved, and when the weight is less than or exceeds the above range, the effect is significantly I have a falling problem.

The composition further includes other additives, and the other additives are excipients, disintegrants and lubricants.

The excipient is selected from the group consisting of Prosolve, starch, microcrystalline cellulose, mannitol, lactose, low-substituted hydroxypropyl cellulose, sucralose and mixtures thereof, specifically Prosolve (Prosolv SMCC90), starch (Starch 1500 ), microcrystalline cellulose (microcrystalline cellulose PH102) and mannitol (Mannitol 160C), but is not limited to the above examples.

The disintegrant is selected from the group consisting of crospovidone, sodium starch gluconicate, croscarmellose sodium, polysorbate, sodium laryl sulfate, and mixtures thereof, and more specifically crospovidone (crospovidone CL-SF), It is not limited to the above examples.

The lubricant is selected from the group consisting of magnesium stearate, light anhydrous silicic acid, talc, glyceryl behenate, and mixtures thereof, and more specifically, magnesium stearate, and is not limited to the above examples.

In the present invention, the extract is extracted using an extraction solvent selected from the group consisting of water, alcohol having 1 to 4 carbon atoms, ether, chloroform, ethyl acetate, dimethyl sulfoxide, and mixtures thereof. The 'daughter-in-law's navel extract' means an extract obtained by extracting from the daughter-in-law's navel.

The daughter-in-law's navel extract is obtained by pulverizing the dried daughter-in-law's navel, water, alcohol having 1 to 4 carbon atoms such as ethanol and methanol, polar solvent such as dimethyl sulfoxide, or a mixing ratio of 1:0.1 to 1:10 of alcohol and water It can be eluted with a mixed solvent having, and more specifically, ethanol can be used as an extraction solvent. At this time, the extraction temperature may be 10 ℃ to 100 ℃, preferably the extract extracted at 25 ℃.

The extraction solvent may be used in an amount of 2 to 50 times, preferably 2 to 20 times, based on the weight of the sample. For extraction, the sample may be left for leaching in the extraction solvent for 1 to 72 hours, specifically 1 to 24 hours.

It may be a product obtained by concentrating the filtered extract under reduced pressure with a vacuum rotary evaporator, but is limited thereto as long as it is a daughter-in-law's navel extract capable of inhibiting the death of skin cells by the chemicals of the present invention and exhibiting skin moisturizing and skin regeneration effects It does not, and includes all extracts, dilutions or concentrates of extracts, dried products obtained by drying extracts, or refined or purified products thereof.

The above dume leek extract, Silgeori tree extract, Dajeongkeom tree extract, and Leol pheasant tree extract can also be prepared using the same method as the daughter-in-law's navel extract.

A cosmetic composition for skin anti-pollution according to another embodiment of the present invention includes the composition.

The cosmetic composition of the present invention may further include components such as water, oil, surfactants, moisturizers, thickeners, lower alcohols, pigments, preservatives, and fragrances, which are ingredients formulated in general skin cosmetics.

In addition, the cosmetic composition may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, It may be formulated as oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it may be prepared in the form of softening lotion, nutrient lotion, nutrient cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the cosmetic of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or oxide as a carrier component Zinc and the like may be used.

In addition, when the cosmetic formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydro It may contain a propellant such as carbon, propane/butane or dimethyl ether.

When the formulation of the cosmetic of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, There are propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the cosmetic of the present invention is a suspension, water as a carrier component, liquid diluents such as ethane or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters such as A suspending agent such as microcrystalline cellulose aluminum metahydroxide bentonite or tracanth may be used.

When the cosmetic formulation of the present invention is surfactant-containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative methyl taurate, and sarcosyl Nate, fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivatives or ethoxylated glyceryl fatty acid esters may be used.

Ingredients included in the cosmetic composition of the present invention include ingredients commonly used in cosmetic compositions, in addition to peptides and carrier components as active ingredients, for example, antioxidants, stabilizers, solubilizers, vitamins, pigments, and fragrances. May contain adjuvants.

A lotion according to another embodiment of the present invention includes the above composition.

According to the composition for skin anti-pollution containing the daughter-in-law's navel extract of the present invention, the effect of suppressing the death of skin cells caused by chemicals while relieving skin irritation using a natural extract harmless to the human body is excellent. In addition, it is possible to provide a composition for skin anti-pollution with excellent skin moisturizing and skin regeneration effects. Furthermore, it is possible to provide a cosmetic composition and lotion having the above effects.

1 is an experimental result of the apoptosis inhibitory effect of skin keratinocytes (HaCaT Cell) of a composition for skin anti-pollution according to an embodiment of the present invention.
Figure 2 is a fibroblast activity test result of the skin anti-pollution composition according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement the present invention. However, the present invention may be embodied in many different forms and is not limited to the embodiments described herein.

[Preparation Example 1: Preparation of natural extract]

The daughter-in-law's navel leaves were pulverized in a blender and then made into powder. The powder sample was completely immersed in 50% ethanol as an extraction solvent at a ratio of 1:10 (w;v), and extracted three times for 3 hours at 80 °C while refluxing. Extract was Whatman No. Filtered with 2 filter paper. The filtrate was concentrated under reduced pressure at 60°C to prepare a daughter-in-law's belly button extract (PE).

Dume leek extract (AE), Silgeoli tree extract (CE), Dajeongkeom tree extract (RE), and Less pheasant tree extract (VE) were prepared in the same way as the method for preparing the daughter-in-law's belly button extract (PE).

[Preparation Example 2: Preparation of Mixed Composition]

Prosolve SMCC90, starch Starch1500, microcrystalline cellulose PH102, mannitol 160C, and crospovidone CL-SF used as a disintegrant were put into a 30 mesh sieve and appled to prepare a mixture, which is a lubricant in the mixture A mixed composition was prepared by adding magnesium stearate and mixing and tableting.

The content of the natural extract included in the mixed composition is shown in Table 1 below.

T1 T2 T3 T4 T5 T6 T7 T8 T9 T10 T11 T12 T13 T14 T15 PE 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 AE - 17 - 13 15 17 20 22 17 17 17 17 17 17 17 CE - - 17 13 15 17 20 22 17 17 17 17 17 17 17 RE - - - - - - - - 7 - 3 5 7 10 12 VE - - - - - - - - - 7 3 5 7 10 12

(unit: parts by weight)

[Experimental Example 1: Skin Cell Apoptosis Inhibitory Effect Experiment]

In general, the degree of cell damage by benzopyrene (2.5ppm) was measured using skin keratinocytes that come into direct contact with pollutants (control group), and T1 to T15 of Preparation Example 2 were treated to benzopyrene (2.5ppm). The degree of cell damage was measured, and by comparing it, the effect of inhibiting skin cell death of the composition was confirmed.

Specifically, 200 μl of dermal keratinocyte HaCaT was dispensed into a 96-well plate at a concentration of 1×10 ⁴ cells/ml and cultured for 24 hours at 37° C. in a CO ₂ incubator. At this time, IMDM medium containing 10% of bovine serum and 1% of antibiotics was used as the medium.

After culturing, the medium was replaced with IMDM medium excluding bovine serum and antibiotics, and then treated with benzopyrene and then treated with T1 to T15 to further culture for 21 hours. Thereafter, a 5 mg/ml MTT solution was added and incubated for 3 hours, then the medium was removed, 150ul of dimethyl sulfoxide was added to each well, shaken for 10 minutes, and absorbance was measured at 570 nm. The results are shown in Table 2 and FIG. 1 below.

Cell death rate (%) Control (treated with benzopyrene only) 60 T1 55 T2 53 T3 56 T4 55 T5 44 T6 40 T7 43 T8 55 T9 54 T10 49 T11 48 T12 35 T13 31 T14 33 T15 49

Based on Table 2, when the compositions of T1 to T15 were treated, it was confirmed that there was an effect of inhibiting the death of keratinocytes in the skin compared to the control group, effectively protecting the skin from chemicals contained in fine dust, etc. It can be seen that it works.

In particular, when the composition of T5 to T7 and the composition of T12 to T14 are treated, it can be seen that the effect of inhibiting the death of the skin keratinocytes is maximized, according to the weight range of the natural extract contained in the composition , it can be seen that the anti-pollution effect is the most excellent.

[Experimental Example 2: Skin moisturizing effect test]

In order to test the skin moisturizing effect of the composition of the present invention, 100 men and women aged 22 to 33 years old (50 men, 50 women) without skin diseases that could affect the test results were tested, and each subject Before applying each composition, the test site was washed with water and allowed to adapt to the external environment for 30 minutes.

Experimental environmental conditions were conducted in the same place where the room temperature was maintained at 22 to 24 ° C and the room humidity was maintained at 45 to 50%. The compositions of T1 to T15 were applied to the cheeks at 2Х2cm ² by 20μL each, and the skin surface conductivity of each application site was measured at 30-minute intervals (0, 30, and 60 minutes) with a Vapometer (Delfin Co.) It is shown in Table 3 below.

For comparison of the moisturizing effect, a 1% aqueous solution of hyaluronic acid known to have an excellent moisturizing effect was used.

0 minutes 30 minutes 60 minutes Control (untreated) 7.0 2.8 1.3 T1 6.8 3.5 2.4 T2 7.1 3.4 2.1 T3 7.0 3.0 2.2 T4 6.9 2.9 2.3 T5 7.0 3.8 3.6 T6 6.8 4.0 3.7 T7 6.9 3.9 3.5 T8 7.1 3.3 2.1 T9 7.2 3.0 2.9 T10 6.7 3.1 2.9 T11 7.0 3.0 2.8 T12 7.1 5.5 4.5 T13 7.0 5.6 4.9 T14 6.9 5.2 4.6 T15 7.2 2.9 2.7

(Unit: g/m ² hr)

Based on Table 3, it can be seen that when the compositions of T1 to T15 are treated, the moisturizing effect is equal to or higher than that of hyaluronic acid.

In particular, when the composition of T5 to T7 and the composition of T12 to T14 are treated, it can be seen that the moisturizing effect is maximized, and when the weight range of the natural extract included in the composition, the skin barrier strengthening and moisture content It can be confirmed that the skin moisturizing power is maximized by increasing

[Experimental Example 3: Skin regeneration effect test]

Cell activity was measured using MTT (3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetra-zolium bromide) reagent to confirm the effect of promoting skin regeneration of the T1 to T15 compositions.

After culturing human-derived fibroblasts and inoculating them in a 96-well plate incubator at a concentration of 1 X 10 ⁵ cells/well, the T1 to T15 compositions were applied for about 24 hours, and then an MTT test was performed to determine the effect of promoting cell activity. The skin regeneration effect was confirmed.

Cell activity was evaluated by comparing the sample with diluted vehicle (DMEM containing 1% FBS serum). As the medium of Comparative Example, DMEM containing 1% FBS serum was used. The results of cell activity were selected according to Equation 1 below, and the test was repeated three times. The results are shown in Table 4 and FIG. 2 below.

[Equation 1]

Cell activity (%) = (reaction absorbance of treatment group - reaction absorbance of control group / reaction absorbance of control group) X 100

Cell activity (%) Control (untreated) 0 T1 15 T2 16 T3 14 T4 19 T5 27 T6 29 T7 28 T8 20 T9 21 T10 22 T11 25 T12 35 T13 38 T14 36 T15 25

Based on Table 4, it can be seen that treatment of the compositions of T1 to T15 has the effect of regenerating the skin by promoting the cellular activity of fibroblasts.

In particular, when the composition of T5 to T7 and the composition of T12 to T14 are treated, it can be seen that the cell activity of fibroblasts is maximized, and when the weight range of the natural extract included in the composition, the skin regeneration effect is It can be seen that the best

[Experimental Example 4: Skin irritation test]

50 males and females aged 20 to 40 were selected, divided into 5 groups of 20 each, and after using lotion containing the T1 to T15 composition, a survey was conducted on whether or not skin irritation occurred, The results are shown in Table 5 below.

[Evaluation standard]

◎: no irritation at all

○: almost no irritation

△: It is slightly irritating, so it is not recommended to use it on sensitive skin

Х: Cannot be used due to severe irritation

group 1 group 2 group 3 group 4 group 5 T1 ○ ○ △ △ ○ T2 ○ △ ○ △ ○ T3 ○ △ △ ○ ○ T4 ○ ○ △ △ △ T5 ◎ ○ ◎ ○ ◎ T6 ◎ ○ ◎ ○ ◎ T7 ◎ ○ ○ ◎ ◎ T8 ○ △ △ △ ○ T9 ◎ ◎ ○ ○ ○ T10 ○ ○ ◎ ◎ ○ T11 ○ ○ ◎ ○ ○ T12 ◎ ○ ◎ ◎ ◎ T13 ◎ ◎ ◎ ○ ◎ T14 ○ ◎ ◎ ◎ ◎ T15 ○ △ ◎ ○ ○

According to Table 5, the lotions containing the compositions of T1 to T15 were generally evaluated as having no severe skin irritation.

In particular, in the case of lotions containing compositions of T5 to T7 and T12 to T14, skin irritation was evaluated as the least, and based on this, the composition of the present invention uses natural extracts that are harmless to the human body, so people with sensitive skin It can be confirmed that it is also effective.

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements of those skilled in the art using the basic concept of the present invention defined in the following claims are also made according to the present invention. falls within the scope of the rights of

Claims (8)

Contains daughter-in-law navel extract as an active ingredient,
To further contain the dume leek extract, the silage tree extract, the sweet tree extract and the lesser pheasant tree extract,
With respect to 100 parts by weight of daughter-in-law's navel extract, 15 to 20 parts by weight of Dume leek extract, 15 to 20 parts by weight of Silgeori tree extract, 5 to 10 parts by weight of Dajeongneum tree extract and 5 to 10 parts by weight of pheasant tree extract
Excellent in inhibiting the death of skin cells by chemicals
A composition for skin anti-pollution comprising a daughter-in-law's navel extract. According to claim 1,
The chemical is
It is selected from the group consisting of polycyclic aromatic hydrocarbons, aldehydes and nitrogen oxides
A composition for skin anti-pollution comprising a daughter-in-law's navel extract. According to claim 1,
The composition has excellent skin moisturizing and skin regeneration effects
A composition for skin anti-pollution comprising a daughter-in-law's navel extract. delete According to claim 1,
The composition further comprises other additives,
The other additives are excipients, disintegrants and lubricants.
A composition for skin anti-pollution comprising a daughter-in-law's navel extract. According to claim 1,
The extract is extracted using an extraction solvent selected from the group consisting of water, alcohol having 1 to 4 carbon atoms, ether, chloroform, ethyl acetate, dimethyl sulfoxide and mixtures thereof
A composition for skin anti-pollution comprising a daughter-in-law's navel extract. A cosmetic composition for skin anti-pollution comprising the composition according to any one of claims 1 to 3, 5 and 6. A lotion comprising a composition according to any one of claims 1 to 3, 5 and 6.

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