KR101119410B1 - Foeniculum vulgar extracts compositions for treating or preventing inflammatory diseases - Google Patents

Abstract

The present invention relates to a composition for treating and preventing inflammatory diseases, which comprises the extract of Feneni (Foeniculum vulgar) as an active ingredient, more specifically, standardized to include a content of caffeine (Caffeine) in a range of fennel extract And standardized and formulated to exhibit excellent inhibitory effects caused by inflammatory changes such as analgesic suppression, acute inflammatory suppression, and acute edema suppression, which can be used as agents useful for treating and preventing diseases caused by inflammatory changes such as arthritis. The present invention relates to a fennel extract.

Description

{Foeniculum vulgar extracts compositions for treating or preventing inflammatory diseases}

The present invention relates to a composition for the treatment and prevention of inflammatory diseases, characterized in that it comprises an extract of Feneni (Foeniculum vulgar) as an active ingredient, more specifically, the content of caffeine (Caffeine) in the fennel extract in a certain range It is standardized, standardized and formulated as much as possible so that the inhibitory effect of inflammatory changes such as analgesic suppression, acute inflammatory suppression and acute edema suppression is excellently expressed, which is useful for treating and preventing diseases caused by inflammatory changes such as arthritis. It relates to a fennel extract that can be used as.

Arthritis is a medical term that collectively refers to the development of inflammatory changes in the joints by some cause, such as bacteria or trauma. Such arthritis is divided into acute and chronic.

Acute arthritis is classified as follows: ① serous arthritis (보 性): Usually caused by trauma (外傷), the cause is unknown, usually occurs in only one joint. ② serous fibrosis (奬 液 纖維素 性) arthritis: Occurs during acute arthritis of arthritis, turbid effusion in the joint cavity (渗出 液) stops. Fibrosis of the stomach membrane (생) is created, even if the inflammation subsides, leaving severe movement disorders. ③ purulent (化膿 性) arthritis: joints in the open window (開放 創) or gonorrhea, typhoid fever, scarlet fever, sepsis (敗血症) is a multiple of infectious diseases. Infants 1 to 2 months of age cause severe bone damage, resulting in incurable dislocation. In adults, periosteal osteomyelitis causes the peas to burst and pus enters the joints. This is called secondary pyogenic arthritis.

Chronic arthritis is classified as follows: ① Specific Inflammation: Tuberculosis, syphilis, or postmenopausal arthritis due to many metabolic disorders of uric acid in middle-aged men. ② Multiple arthritis: Chronic arthritis is caused by a lot of arthritis in acute serous arthritis or (TB) syphilis or gonorrhea in the course of multiple manifestations of sepsis. In addition, it also includes arthritis called Still's disease. ③ deformable osteoarthritis: bone or joint aging or trauma is the cause. ④ hemophilia (血友病 性) arthritis: when hemophilia is caused by bleeding in the joints.

Many drugs have been developed for the purpose of treating diseases caused by inflammatory changes typified by arthritis as described above.

On the other hand, the root of the fennel in the name of the herbal medicine is called fennel. Originating from the Mediterranean coast, the generic name Peniculum comes from the Latin foenum, which means `` hay, '' which originates from the peculiar smell of hay. The height is 1.5 ~ 2m, the stem grows straight and the inside is empty. The leaves are long sheath-shaped, pointed at the end, and divided into three to four branches like feathers. In early summer, small yellow flowers bloom in the shape of umbrellas at the end of branches. Flowers have yellow pollen bags and hydroponics grow a little dry inside. The pistil is very small in two and bends back as it grows. The fruit is split and has a sweet taste and aroma.

The root of the fennel is called fennel muscle, which is used for medicines such as whole body, creeping dog, gutung, dry cow, hookworm, expectorant, nausea, pain, each, swelling, neuralgia, deadly poison, epilepsy, crayfish, arthritis. It contains methyl chavicol, anethole, fencone ((+)-fenchone), sugars, saponins, and amino acids. The leaves contain lactones, phenols, triterpenoids, amino acids and organic acids.

 In addition, the traditional medicinal herbs and related documents such as Dongbobom, Hyangjegbang, and light-dose are prescribed as unidirectional herbal medicines. However, these prescriptions are used to distinguish the morphology of fennel muscles and to prepare herbal medicines and fluids. It was just a brief comment on the method. In other words, opinions regarding the active ingredients expressing the efficacy of the components extracted through the above-described method could not be obtained.

In addition, it is difficult to standardize extracts because fennel muscle has a large variation in the content of the indicator components by the place of origin and harvest time, and commercialization is difficult because it is impossible to reproducibly obtain an active fraction with excellent activity such as anti-inflammatory pain, improved blood circulation, and arthritis treatment. It also remains a problem to be solved.

In order to solve the above problems of the inventors of the present invention, research efforts to achieve the standardization of the fennel extract according to the specific components and its content control, as a result of the anti-inflammatory analgesic as a suppression of symptoms by inflammatory changes In addition to the effects of inhibiting immune cell proliferation, acute inflammation inhibition, chronic inflammation inhibition and acute edema, the standardized fennel extract was completed with the present invention.

According to the research results of the present invention, it was found that the fennel extract containing a certain amount of caffeine (Caffeine) of the fennel extract can be easily and reproducibly obtained, and thus commercialized.

Therefore, an object of the present invention is to provide a pharmaceutical composition and a food composition for the treatment and prevention of inflammatory diseases, including fennel muscle extract as an active ingredient.

In addition, an object of the present invention is to provide a baekduong extract containing the content of caffeine (Caffeine) in a specific range as an indicator.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.

In order to achieve the above object, the present invention provides a pharmaceutical composition for treating and preventing inflammatory diseases comprising a caffeine extract as an active ingredient.

In addition, the present invention provides a food composition for treating and preventing inflammatory diseases, including fennel muscle extract as an active ingredient.

In addition, the present invention provides a extract of Foeniculum vulgar containing 0.5 to 5.0% by weight of caffeine (Caffeine) as an indicator.

According to the present invention, the extract of Fennel Root was very effective in inhibiting inflammation in TPA-induced edema, arachidonic acid-induced edema, and carrageenan-induced acute foot edema. , Acute inflammatory inhibitors, chronic inflammatory inhibitors, and acute edema inhibitors were excellent, and standardization could be achieved by controlling the content of caffeine as an indicator substance. Therefore, the extract of fennel muscle according to the present invention is prepared by extracting from fennel muscle and selecting caffeine (Caffeine) as an indicator material and standardizing it by controlling the content of the indicator material to prepare inflammatory disease treatment agents and health functional foods such as arthritis drugs. Can be.

In addition, the composition of the present invention is excellent in safety because it contains a fennel muscle extract has been used as a herbal medicine for a long time as an active ingredient.

Figure 1 shows the HPLC chromatogram of fennel muscle extract.
Figure 2 is a TPA-induced mouse edema inhibition test (TPA-induced mouse ear edema.) Of fennel muscle extract.
Figure 3 is arachidonic acid-induced ear edema inhibition test (Arachidonic acid-induced mouse ear edema assay) of fennel muscle extract.
Figure 4 is an acetic acid-induced writhing response in mice.
5 is a test for treating acute arthritis of fennel muscle extract.

Hereinafter, the present invention will be described in more detail.

The present invention is to standardize and standardize the extract of fennel muscle so that the content of caffeine in a certain range, suppress the symptoms caused by inflammatory changes such as analgesic suppression, acute inflammation inhibitory, chronic inflammation inhibitory, acute edema inhibition and chronic edema inhibitory The present invention relates to a fennel extract which is excellent in expression and useful for the treatment and prevention of inflammatory diseases such as arthritis.

Since the content of the active bioactive substance contained in the raw herbal medicine of fennel muscle may vary greatly depending on the place of origin, the harvesting time, the storage period and the storage state, the composition of the herbal composition may not be limited to the weight ratio of the ingredients used. It is more preferable to limit the content. In addition, according to the present invention shows a significant difference in the biological activity, such as joint protection according to the content of the effective bioactive material.

Therefore, in the present invention, caffeine has not been proposed as a marker for obtaining fennel muscle extract maximizing drug expression. As an active ingredient of the medicament obtained in the present invention, the action of other components other than the above components cannot be excluded.

The fennel muscle extract used as a medicament for the treatment of diseases caused by inflammatory changes of the present invention can be selected by extracting the specific active fraction from the extract itself (herbal extract) or fennel extract extracted from the fennel root. In this case, the blending ratio is preferably determined by the content of caffeine, which is an indicator substance, regardless of the content of fennel muscle, which is a crude drug. Caffeine, the indicator substance, is preferably contained at least 0.5% by weight, more preferably 0.5 to 5.0% by weight, more preferably 1.0 to 2.0% by weight of fennel extract. It is most preferable to contain%. If the caffeine content is less than 0.5% by weight, relatively poor results for the treatment of arthritis and the like. There is no particular limitation on the upper limit of the content of caffeine, but the content of caffeine in excess of 5.0% by weight does not increase the effect anymore and is preferable in terms of technical and economical preparation. Tend not to.

Caffeine (Caffeine) selected as the indicator material in the present invention is an important component of the drug for treating diseases caused by inflammatory changes of the present invention, when contained in a certain content range can express a more potent drug effect by expressing a synergistic effect . As an active ingredient of the fennel muscle extract obtained in the present invention, it is presumed to exhibit a therapeutic and prophylactic effect of inflammatory diseases as an action of other components besides caffeine. That is, as an active ingredient of the drug obtained in the present invention, the action of other components other than the above components cannot be excluded.

The present invention provides a fennel extract, characterized in that extracted by selecting one from the crowd consisting of water, alcohol and alcohol aqueous solution.

The extract is,

1) extracting, cooling and filtering by selecting one from a crowd consisting of 5 to 8 times the weight of fennel root protozoa, water, alcohol and aqueous alcohol solution,

2) separating the filtrate obtained above into an aqueous alcohol solution and concentrating under reduced pressure at 50 to 90 ° C., and

3) to the concentrate obtained in the above, it is prepared, characterized in that it comprises the step of centrifuging at 700 ~ 1,200 rpm after the addition of alcohol.

The preparation method of the extract is described in more detail as follows.

1) Extracted, cooled and filtered with an aqueous solution of 6-8 times the weight of the fennel root medicinal herbs, washed, dried and cut, and filtered, and again 5-5 times the water or alcohol aqueous solution of the mixed herbal weight. Filtering by warming and re-extracting, followed by filtration with a previous filtrate; 2) separating the filtrate obtained in 1) into the same amount of an aqueous solution of alcohol and then concentrating under reduced pressure at 50 to 90 ° C., and 3) distilling the alcohol recovered in the concentrate of 2) under reduced pressure. Concentration under reduced pressure, vacuum drying, grinding and sterilizing the filtrate centrifuged at 700 ~ 1,200 rpm after the addition.

To explain this in detail, washing and drying, adding water or alcohol aqueous solution to the fine fennel muscle probiotic, repeated extraction for 2 to 4 hours, 2 to 4 times, slowly cooled to room temperature and then centrifugation Filter and separate from the residue. To the residue is added 6-8 times the amount of water or alcohol aqueous solution of the weight of the mixed herbal medicine, heated and re-extracted, filtered and then mixed with the previous filtrate and filtered. By adding water or an aqueous alcohol solution to the residue as described above, the extraction efficiency can be increased by re-extraction and filtration. At this time, if the amount of the water or alcohol aqueous solution used for extraction is too small, the solubility of the extract is poor, the extraction efficiency is reduced, if the amount is too large, the amount of the aqueous alcohol solution used for layer separation increases, it takes a long time under reduced pressure concentration, etc. May cause economic or handling problems.

In the present invention, the method of re-extracting after the first extraction is adopted. Even in the case of mass production of the herbal extracts, even though the filtration is effective, the loss occurs because the water content of the herbal medicine itself is high, so the extraction efficiency is reduced only by the first extraction. This is to prevent this. In addition, as a result of verifying the extraction efficiency of each step, it was found that about 80 to 90% of the total extraction amount is extracted by the second extraction, and the third or more multistage extraction is not economical.

As described above, the extract obtained by extraction with an aqueous solution of water or alcohol over the first and second stages is filtered and concentrated, and then purified from impurities such as unnecessary proteins, polysaccharides and fatty acids contained in the filtrate, in the present invention, the same amount of alcohol as the filtrate. The impurities are purified by separating the aqueous solution using a solvent two to five times to obtain a solvent fraction.

The alcohol is preferably an alcohol having 1 to 6 carbon atoms, more preferably a 30% aqueous ethanol solution. When the amount of the lower alcohol solution is less than the filtrate, fine particles formed by unnecessary components such as fatty acids are formed. Not only is it difficult to separate, but the extraction content of the active ingredient is lowered, which is not efficient.

The alcohol may be used both aliphatic and aromatic alcohols commonly used in the art, it is preferable to use a lower alcohol having 1 to 6 carbon atoms than aliphatic alcohol.

The layered extract was concentrated under reduced pressure at 50 to 90 ° C. to remove residual solvent. The obtained concentrate was added with alcohol recovered at the time of concentration under reduced pressure, and then the filtrate was centrifuged at 700 to 1,200 rpm. It is cloudy and then concentrated under reduced pressure again.

The concentrate thus obtained is vacuum dried at 0.08 to 0.3 pa at 50 to 90 ° C., and then pulverized and sterilized to 30 to 200 mesh to obtain a powdery fennel extract, which is used for treating and preventing inflammatory diseases such as arthritis. Since it is excellent, the composition containing this extract is expected to be useful as a therapeutic agent for inflammatory diseases such as joint protectants.

The fennel muscle extract according to the present invention is formulated in a conventional manufacturing method to produce tablets, capsules, injections, etc. Among them, the sum of lactose, microcrystalline cellulose, magnesium stearate, and the like, used as a base for preparing tablets, and the fennel muscle When the extract is used in a ratio of 1: 1, a tablet having an activity for treating diseases caused by inflammatory changes such as arthritis can be prepared.

In the preparation of such pharmaceuticals, herbal extracts may be used as such, but they may be mixed with pharmaceutically acceptable carriers, excipients, diluents, etc. to prepare powders, granules, capsules or injections. Is possible. In addition, the extract of fennel muscle according to the present invention has been used for food and medicinal use since ancient times, and there is no particular restriction on its dosage, and the body absorbency, weight, age, sex, health condition, diet, administration time, administration of the patient. It may vary depending on the method, the rate of excretion, the severity of the disease, and the like. In general, the extract of fennel muscle is preferably administered about 0.1 ~ 10 mg per 1kg body weight.

Therefore, the composition containing the active ingredient of the present invention is to be prepared in consideration of the effective amount range, and the unit dosage form formulated in this way according to the judgment of the expert and the needs of the individual to monitor or observe the administration of the drug as needed Specialized medications can be used or administered at regular intervals.

According to another aspect of the present invention, the present invention provides a food for preventing and treating inflammatory diseases, in particular a health functional food, containing fennel muscle extract as an active ingredient.

In the present invention, "health functional food" refers to a food having a bioregulatory function such as prevention and treatment of diseases, biological defense, immunity, recovery from illness, and aging inhibition, and should be harmless to the human body when taken in the long term.

In order to use the fennel muscle extract of the present invention for the prevention and treatment of inflammatory diseases as described above, it can be prepared by a variety of methods known in the food science or pharmaceutical field, or by itself or a food acceptable carrier, excipient, diluent It can be prepared in any food form that can be ingested orally by mixing with the back. Preferably in the form of beverages, pills, granules, tablets or capsules.

The health functional food of the present invention may further include ingredients that are commonly added during food production and are food acceptable. For example, when the beverage is prepared, in addition to the plant extract of the present invention, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, fruit juice, and the like may be further included.

The amount that can be included as an active ingredient of the health functional food according to the present invention may be appropriately selected according to the age, sex, weight, condition, symptoms of the disease of the person who wants to prevent and treat inflammatory diseases, preferably 1 day for adults It is good to include about 0.01g to 10.0g, the anti-inflammatory effect can be obtained by eating a health functional food having such a content.

Hereinafter, the present invention will be described in more detail with reference to the following examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

Example  : Fennel  Preparation of Extract

After washing with water and drying well, the fennel muscle was added with 30% ethanol aqueous solution, and the hot water was extracted twice every 2 hours while stirring well. The extract was slowly cooled to room temperature and then centrifuged to remove debris, and the filtrates were combined and concentrated under reduced pressure at 50 to 90 ° C.

After the ethanol recovered through the ethanol fraction was added to the concentrate, the concentrate was sufficiently suspended, and the filtrate was centrifuged at 5000 rpm. The filtrate was concentrated under reduced pressure and vacuum dried at 60 ° C. and 0.08 pa. Sterilization was performed through a grinding process. The caffeine content in the fennel extract extracted and purified by the above method was 1.55% by weight. In addition, the fennel muscle extract obtained by the above method was analyzed by HPLC under the following conditions.

   1) Solvent:

   20: 1: 79 (Methanol 100%: Acetic acid: Water)

   2) Column: C18 reverse phase (Agilent Eclipse XDB-C18 / 1.75㎛, 2.1 * 50mm)

   3) flow rate: 1 ml / min

   4) Column temperature: 35 ℃

   5) Detector: UV 280 nm

Experimental Example  One : TPA  Judo Edema  Suppression test ( TPA - induced mouse ear edema .)

TPA-induced mouse edema inhibition test (TPA-induced mouse ear edema.) Was performed on the fennel muscle extract prepared by the above example, and the results are shown in Table 1 and FIG. 2.

Experimental Method 1

Seven-week-old ICR mice were isolated for each experimental group, followed by TPA (1 hour after oral administration of 200 mg / Kg of joining tablets (SK Chemical, Joins-tab.) And 100, 200, 400 mg / Kg of fennel extract. 2.5 μg / 20 μl) was dissolved in acetone and induced edema in the ears of rats. In inducing edema, the experimenter completely secured the subject from behind and the second experimenter stimulated the edema causing ear in the ear by using a micro pipet. Four hours later, ear edema was measured for each experimental group. The measurement was carried out using the tibial decay method to induce a precise measurement after culling the specimen.

process Thickness of the ear Inhibition degree of inflammation (%) Non-treatment 0.38 ± 0.09 0 % Joining tablets (200mg / kg) 0.30 ± 0.02 20.87% Fennel Muscle Extract (100mg / kg) 0.30 ± 0.01 20.87% Fennel Extract (200mg / kg) 0.31 ± 0.02 18.26% Fennel Muscle Extract (400mg / kg) 0.30 ± 0.03 20.87% 1.Data represent the mean of difference in ear thickness (mm) ± SEM (n = 12)
2. No treatment: No treatment of drug after inducing edema

As shown in Table 1, when the fennel muscle extract prepared by the present invention was used, the TPA-induced ear edema inhibitory effect was excellent. It can be seen that it is preferable.

Experimental Example  2 : Arachidonic acid ( Arachidonic acid ) Induced ear edema suppression test ( Arachidonic acid - induced mouse ear edema assay )

Arachidonic acid-induced mouse ear edema assay was performed on the fennel muscle extract prepared by the above example, and the results are shown in Table 2 and FIG. 3.

Experimental Method 2

Seven-week-old ICR mice were isolated for each experimental group, followed by oral administration of 200 mg / Kg of joining tablets (SK Chemical, Joins-tab.) And 100, 200, 400 mg / Kg of fennel muscle extracts. It was dissolved in acetone (2 mg / 20 μl) to cause edema in the ear. In inducing edema, the experimenter completely secured the subject from behind and the second experimenter stimulated the edema causing material in the ear by using a micropipette. After 1 hour, ear edema was measured for each experimental group. The measurement was carried out using the tibial decay method to induce a precise measurement after culling the specimen.

process Thickness of the ear Inhibition degree of inflammation (%) Non-treatment 0.30 ± 0.01 0 % Joining tablets (200mg / kg) 0.27 ± 0.01 8.89% Fennel Muscle Extract (100mg / kg) 0.26 ± 0.04 13.33% Fennel Extract (200mg / kg) 0.26 ± 0.01 13.33% Fennel Muscle Extract (400mg / kg) 0.28 ± 0.01 6.67% 1.Data represent the mean of difference in ear thickness (mm) ± SEM (n = 12)
2. No treatment: No treatment of drug after inducing edema

As shown in Table 2, when the fennel muscle extract prepared according to the present invention is used, the arachidonic acid-induced ear edema suppression effect is excellent, and in particular, when the concentration of the fennel extract 200 mg / kg, the arachidonic acid-induced ear edema inflammation inhibition rate is most It can be seen that the preferred.

Experimental Example  3: acetic acid induction stretching inhibition test ( Acetic acid - induced writhing response in mice .)

The acetic acid-induced stretching inhibitory test (Acetic acid-induced writhing response in mice) was performed on the fennel muscle extract prepared by the above example, and the results are shown in Table 3 and FIG. 4.

Experimental Method 3

In this experiment, 7-week-old ICR mice were isolated for each herbal candidate by referring to the method of "Siegmund", and then 200 mg / Kg of joining tablets (SK Chemicals, Joins-tab.), Fennel extract 100, 200, 400 Inflammation of the organ was induced by intraperitoneal administration of acetic acid (0.075%) (0.1 ml / 10 g) 1 hour after oral administration of mg / Kg. After 10 minutes, the mice were stretched for 10 minutes because the swelling causes itching, which can be used to estimate the degree of pain.

process Number of twists
(Writhing number) Pain control degree (%) Non-treatment 28.67 ± 4.73 0 % Joining tablets (200mg / kg) 4.67 ± 4.20 83.72% Fennel Muscle Extract (100mg / kg) 18.33 ± 3.06 36.05% Fennel Extract (200mg / kg) 13.33 ± 7.23 53.49% Fennel Muscle Extract (400mg / kg) 12.67 ± 3.79 55.81% 1.Data represent the mean of difference in ear thickness (mm) ± SEM (n = 12)
2.No treatment: After treatment of stretching, no treatment treatment

As shown in Table 3, the use of the fennel muscle extract prepared according to the present invention is excellent in acetic acid-induced stretching inhibitory effect, especially when the concentration of fennel extract 400 mg / kg is the most preferred acetic acid-induced stretching inhibitory effect Can be.

Experimental Example  4: Acute Arthritis Treatment Effect Test

Acute arthritis treatment effect assay was performed on the fennel muscle extract prepared by the above example, and the results are shown in Table 4 and FIG. 5.

Experimental Method 4

Male SD rats (about 200 g) were orally administered with 200 mg / Kg of joining tablets (SK Chemical, Joins-tab.), 1 ml of fennel muscle extract 100, 200, 400 mg / Kg and 100% of 1% carrageenan saline solution. Was injected into the left plantar to induce acute foot edema. Edema was measured 4 hours later using a plethysmometer (LE 7500).

process Edema growth rate (%) Edema inhibition degree (%) Non-treatment 43.29 0 Joins (200mg / kg) 2.70 20.03 Fennel Muscle Extract (100mg / kg) 92.69 6.30 Fennel Extract (200mg / kg) 37.57 17.77 Fennel Muscle Extract (400mg / kg) 22.48 23.42 1.Data represent the mean of difference in ear thickness (mm) ± SEM (n = 12)
2.Treatment-free: No treatment of drug after induction of edema

As shown in Table 4, when the fennel muscle extract prepared according to the present invention is used, the acute arthritis therapeutic effect is excellent, and the acute arthritis therapeutic effect is particularly preferable when the fennel muscle extract concentration is 400 mg / kg. .

Experimental Example  5: NO  Impact on

Acute arthritis therapeutic effect assay was performed on the fennel muscle extract prepared by the above example.

Experimental Method 5

After 24 hours of treatment with the raw 264.7 cell line derived from mouse macrophages with LPS (1 ㎍ / ml), the culture medium was collected and nitric oxide, an inflammation-inducing molecule in the body, using enzyme and radical chromatography. , The effect of fennel extract on the production of NO) was investigated. For measurement of NO, 50 μl of the culture solution was taken, and 25 μl of Greases solution 1 and 2 were mixed to measure absorbance at 540 nm. Absorbance values were measured using an ELISA kit (R & D systems, USA).

Fennel
extract
(占 퐂 / ml)
0
0.5
5
50
500 Result Contents Absorbance % Inhibition Absorbance % Inhibition Absorbance Suppression rate
(%) Absorbance Suppression rate
(%) Absorbance Suppression rate
(%) shame 0.579 ± 0.011 0 0.410 ± 0.028 29.14 0.437 ± 0.023 24.48 0.414 ± 0.006 28.45 0.382 ± 0.019 33.98

After treatment with LPS (1㎍ / ml) and fennel muscle extract (0, 0.5, 5, 50, 500 ㎍ / ml) on Raw 264.7 cell line, a mouse-derived macrophage, the cultures were collected and enzymatically analyzed. As a result of quantifying NO, which is an inflammation-inducing molecule in the body, LPS effectively inhibited NO production of macrophages by LPS in cell lines.

Manufacturing example  1: preparation of tablets

Using the fennel muscle extract prepared according to the embodiment of the present invention, the tablet for oral administration was prepared using the wet granulation method and the dry granulation method with the following composition.

[Furtherance]

Fennel extract 200 mg, hard silicic acid 10 mg, magnesium stearate 2 mg, microcrystalline cellulose 50 mg, starch glycolate 25 mg, lactose 101 mg, povidone 12 mg, ethanol anhydrous amount.

Manufacturing example  2: Preparation of Ointment

Using the fennel muscle extract prepared according to the embodiment of the present invention was prepared an ointment with the following composition.

[Furtherance]

Fennel root extract 5 g, cetyl palmitate 20 g, cetanol 40 g, stearyl alcohol 40 g, myristan isopropyl 80 g, monostearic acid sorbitan 20 g, polysorbate 60 g, paraoxybenzoic acid propyl 1 g, 1 g of methyl paraoxybenzoate, phosphoric acid and purified water.

Manufacturing example  3: Preparation of Injection

Using the fennel muscle extract prepared according to the embodiment of the present invention was prepared an injection with the following composition.

[Furtherance]

Fennel root extract 100 mg, mannitol 180 mg, monohydrogen phosphate 25 mg, purified water for injection 2974 mg

Manufacturing example  4 : Transdermal  Produce

Using a fennel muscle extract prepared according to an embodiment of the present invention was prepared a transdermal agent with the following composition.

[Furtherance]

0.4 g of fennel extract, 1.3 g of sodium polyacrylate, 3.6 g of glycerin, 0.004 g of aluminum hydroxide, 0.2 g of methyl paraben, 14 g of water.

Manufacturing example  5: preparation of beverages

Using the fennel muscle extract prepared according to an embodiment of the present invention to prepare a beverage as follows. Purified water is mixed with 200 mg of fennel root extract, 75 mg of vitamin C, 4 mg of vitamin B2, 3 mg of vitamin B6, dietary fiber 1,000 mg, liquid fructose 20000 mg, emulsifier 100 mg, and 50 mg of fragrance. The final mixture was clarified by passing the mixed solution through a filter of 2 ~ 3㎛ before sterilization at 90 ~ 93 ℃ for 15 ~ 20 seconds, filled into 50ml bottle and sterilized after 15 ~ 20 minutes at 80 ~ 85 ℃. Completed.

Claims (14)

delete delete delete delete delete delete delete 1) extracting, cooling and filtering by selecting one from a crowd consisting of 5 to 8 times the weight of fennel root protozoa, water, alcohol and aqueous alcohol solution,
2) The filtrate obtained above was separated with an aqueous solution of alcohol and concentrated under reduced pressure at 50 to 90 ° C.
Concentrating the caffeine containing 0.5 to 5.0% by weight with caffeine (Caffeine) as an indicator, and
3) A method of producing a extract of Feneni (Foeniculum vulgar) using a caffeine (Caffeine) as an indicator, comprising the step of centrifuging at 700 ~ 1,200 rpm after the addition of alcohol to the concentrate obtained above.
delete delete delete delete delete delete

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