KR100932836B1 - Snow Cordyceps Sinensis Extract Inhibits the Activity of Kadexin - Google Patents
Snow Cordyceps Sinensis Extract Inhibits the Activity of Kadexin Download PDFInfo
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Abstract
본 발명은 골다공증에 관여하는 효소인 카뎁신 K(Cathepsin K)의 활성을 억제하는 작용을 하는 눈꽃 동충하초 추출물 및 그 추출물의 제조방법에 관한 것으로, 보다 상세하게는 눈꽃 동충하초에 증류수를 첨가하여 90 내지 100℃에서 가열 (끊는 물에 중탕)하고, 중탕된 용액을 원심분리하여 상등액을 취하고, 상기 상등액을 동결건조한 추출물로서 카뎁신 K효소의 활성을 억제하여 골다공증을 예방하고 치료할 수 있는 물질과 이의 제조방법에 관한 것이다.The present invention relates to a snow Cordyceps sinensis extract having a function of inhibiting the activity of an enzyme involved in osteoporosis (Cathepsin K) and a method for preparing the extract, and more specifically, by adding distilled water to Snow Cordyceps sinensis 90 to Subsequently, the supernatant was taken by centrifugation of the bathed solution, and the supernatant was lyophilized to inhibit the activity of the Kadepsin K enzyme and to prepare and treat osteoporosis. It is about a method.
이러한 본 발명은, 눈꽃 동충하초 추출물은 카뎁신 K 효소 활성을 효과적으로 억제하는 효과가 있으며, 상기 눈꽃 동충하초 추출물의 유효성분을 포함하여 약 또는 기능성식품을 만들면 골다공증 예방 및 치료에 효과적으로 이용할 수 있다. 특히, 칼슘제제에 상기 눈꽃 동충하초 추출물의 유효성분을 추가하는 경우 골다공증 예방 및 치료를 위한 의약품을 만들 수 있다.
Such a present invention, the Snow Cordyceps sinensis extract has an effect of effectively inhibiting the activity of the Kadepsin K enzyme, and can be effectively used for the prevention and treatment of osteoporosis by making a medicine or functional food containing the active ingredient of the Snow Cordyceps sinensis extract. In particular, when adding the active ingredient of the snowflake Cordyceps sinensis extract to the calcium preparation can make a medicine for the prevention and treatment of osteoporosis.
눈꽃 동충하초, 카뎁신K, 골다공증Snow Cordyceps sinensis, Kadipsin K, Osteoporosis
Description
도1는 본 발명에 따른 일실시예에 있어서, 눈꽃 동충하초 추출물의 농도별 카뎁신 K 활성을 나타낸 그래프,1 is a graph showing the kadipsin K activity according to the concentration of Snow Cordyceps Sinensis extract in one embodiment according to the present invention;
도2은 본 발명에 따른 일실시예에 있어서, 눈꽃 동충하초 추출방법에 따른 카뎁신 K 활성을 나타낸 그래프,Figure 2 is a graph showing the Kadipsin K activity according to the method of extracting snow Cordyceps sinensis according to an embodiment of the present invention,
도3은 본 발명에 따른 일실시예에 있어서, 눈꽃 동충하초의 음이온교환수지 분획물의 카뎁신 K 활성을 나타낸 그래프.
Figure 3 in one embodiment according to the present invention, a graph showing the Kadipsin K activity of the anion exchange resin fraction of Snow Cordyceps sinensis.
본 발명은 골다공증에 관여하는 효소인 카뎁신 K(Cathepsin K)의 활성을 억제하는 작용을 하는 눈꽃 동충하초 추출물 및 그 추출물의 제조방법에 관한 것으로, 보다 상세하게는 눈꽃 동충하초에 증류수를 첨가하여 90 내지 100℃에서 가열(끊는 물에 중탕)하고, 중탕된 용액을 원심분리하여 상등액을 취하고, 상기 상등액 을 동결건조한 추출물로서 카뎁신 K효소의 활성을 억제하여 골다공증을 예방하고 치료할 수 있는 물질과 이의 제조방법에 관한 것이다.The present invention relates to a snow Cordyceps sinensis extract having a function of inhibiting the activity of an enzyme involved in osteoporosis (Cathepsin K) and a method for preparing the extract, and more specifically, by adding distilled water to Snow Cordyceps sinensis 90 to Subsequently, the supernatant is taken from the heated solution at 100 ° C (collapsed in water) and centrifuged to remove the supernatant, and the supernatant is a freeze-dried extract that inhibits the activity of the kadepsin K enzyme and prepares the same. It is about a method.
골다공증을 비롯한 골대사 관련 질환은 생활수준의 향상, 노인인구의 증가로 인해 매년 증가될 것으로 예상된다. 영국의 경우 매년 15만명 정도가 골다공증에 의한 골절이 일어나며, 이로인해 매년 7억5천만 파운드가 지출된다고 한다. 미국의 경우 인구의 10%에 해당하는 2천5백만명의 골다공증 환자 중 매년 130만명 이상이 골절로 고생한다고 하며, 이와 관련된 치료비 규모가 200억 달러에 달한다고 한다. 우리나라의 경우 아직 정확한 통계자료가 없으나 골다공증 환자는 약 200만명으로 추정된다.Osteoporosis-related diseases, including osteoporosis, are expected to increase every year due to improved living standards and an increase in the elderly population. In the United Kingdom, about 150,000 people suffer from osteoporosis every year, which costs £ 750 million a year. In the United States, more than 1.3 million of the 25 million osteoporosis patients, or 10 percent of the population, suffer from fractures each year. In Korea, there are no accurate statistics yet, but about 2 million osteoporosis patients are estimated.
초기에는 아무런 증세도 없이 진행되는 골다공증은 45세 여성의 50%에서 진행되며, 75세이상 여성의 90%는 심한 골다공증이 있다고 한다.Osteoporosis, which progresses initially without any symptoms, occurs in 50% of women aged 45 years and 90% of women over 75 years of age have severe osteoporosis.
카뎁신 K는 최근에 발견된 papain계열의 cystein protease로서, 골조직의 재흡수에 관여하는 중요한 단백질이다(Bromme et al., 1995, Bossard et al., 1996). Bone Remodeling에서 재흡수 단계는 뼈의 표면에 부착되어 있는 파골세포에 의해 이루어진다. 파골세포는 낮은 pH를 유지하고 있는 resorption pit에 카뎁신 K와 같은 단백분해효소를 분비한다(D'Alessio et al., 1999). 카뎁신 K는 효과적인 collagenase로서 typeI과 typeII 콜라겐을 절단한다(Bromme et al., 1999). 골다공증은 파골세포에서 뼈의 재흡수 속도가 조골세포에서의 뼈의 생성 속도보다 빠르게 나타나므로 카뎁신 K의 이런 작용은 골다공증을 치료하기 위한 효과적인 drug target으로 생각되고 있다. 그래서 현재 골다공증을 예방, 치료하기 위해서 카뎁 신 K에 대한 억제물질을 찾기 위한 연구가 진행되고 있다.Kadipsin K is a recently discovered papain family cysteine protease and is an important protein involved in bone resorption (Bromme et al., 1995, Bossard et al., 1996). In bone remodeling, the resorption step is caused by osteoclasts attached to the bone surface. Osteoclasts secrete proteolytic enzymes such as kadipsin K into the resorption pit, which maintains low pH (D'Alessio et al., 1999). Kadipsin K is an effective collagenase that cleaves type I and type II collagen (Bromme et al., 1999). Osteoporosis appears to be an effective drug target for the treatment of osteoporosis because osteoporosis occurs in bone resorption faster than in osteoblasts. In order to prevent and treat osteoporosis, research is being conducted to find an inhibitor for kadipsin K.
현재까지 알려진 카뎁신 K의 억제물질은 alkoxymethylketone(Marquis, Bioorg. Med. Chem., 1999, 7, 581-588), NO donors(Percival, Biochemistry, 1999, 38, 13574-13583), 카뎁신 K의 propeptide(Billington, Biochem. Biophy. Res. Comm., 2000, 276, 924-929)등이 알려져 있다.
To date, known inhibitors of cardipine K include alkoxymethylketone (Marquis, Bioorg. Med. Chem., 1999, 7, 581-588), NO donors (Percival, Biochemistry, 1999, 38, 13574-13583), propeptide (Billington, Biochem. Biophy. Res. Comm., 2000, 276, 924-929) and the like.
본 발명은 골다공증에 효과를 나타내는 눈꽃 동충하초 추출물 및 그 제조방법을 제공하며, 상기 눈꽃 동충하초 추출물을 유효성분으로 함유하는 조성물을 제공하는 것을 목적으로 한다.
An object of the present invention is to provide a snow extract Cordyceps sinensis extract and a method for producing the same, and to provide a composition containing the snow extract Cordyceps sinensis extract as an active ingredient.
본 발명에 의한 눈꽃 동충하초 추출물은, 눈꽃 동충하초로부터 추출되며 카뎁신 K효소의 활성을 저해하는 것을 특징으로 한다.Snowflake Cordyceps sinensis extract according to the present invention is extracted from Snowflower Cordyceps sinensis and is characterized in that it inhibits the activity of the Kadepsin K enzyme.
본 발명에 의한 골다공증 예방 및 치료용 조성물과 기능성 식품은 상기 눈꽃 동충하초 추출물을 유효성분으로 포함한다.The composition and functional food for preventing and treating osteoporosis according to the present invention comprises the snow cauliflower Cordyceps sinensis extract as an active ingredient.
한편, 본 발명에 의한 눈꽃 동충하초 추출물의 제조방법은, 눈꽃 동충하초에 증류수를 첨가하여 90 내지 100℃에서 가열하고, 가열된 용액을 원심분리하여 상등액을 취하고, 상기 상등액을 동결건조하는 것을 특징으로 한다.On the other hand, the method for producing a snowflake Cordyceps sinensis extract according to the present invention, by adding distilled water to the snowflake Cordyceps sinensis, heated at 90 to 100 ℃, by centrifuging the heated solution to take a supernatant, characterized in that the supernatant is lyophilized. .
또한, 본 발명에 의한 눈꽃 동충하초 추출물은, 눈꽃 동충하초에 에탄올을 첨가하여 60℃에서 가열하고, 가열된 용액을 원심분리하여 상등액을 취하고, 상기 상등액을 동결건조하여 제조할 수 있다.In addition, the snow Cordyceps sinensis extract according to the present invention can be prepared by adding ethanol to Snow Cordyceps sinensis and heating at 60 ° C., taking the supernatant by centrifuging the heated solution, and freezing-drying the supernatant.
본 발명에서 카텝신 K 억제 효과가 있는 조성물을 제조하는데 이용되는 눈꽃 동충하초(Peacilomyces japonica)는 가을에 숲 속 낙엽 속의 인시류의 번데기 등에 기생하여 성장하고, 한국·일본·네팔 등지에 분포한다. 눈꽃 동충하초 (Peacilomyces japonica)는 높이 1~4cm로 산호형의 자실체를 갖는데, 자실체는 황갈색의 분생자병속과 산호형의 두부로 이루어진다. 그 두부는 흰 눈꽃이 만발한 듯한 부말상의 백색 분생자로 덮여 있다(박완희, 한국의 버섯, 2002, 교학사). Peacilomyces japonica used to prepare a composition having a cathepsin K inhibitory effect in the present invention is parasitic in the pupa of the deciduous species in the forest in autumn and grows, and is distributed in Korea, Japan, Nepal and the like. Snowflake Cordyceps ( Peacilomyces japonica ) has a height of 1 ~ 4cm and has a coral-like fruiting body, which is composed of a yellow-brown conidia and coral-like tofu. The tofu is covered with white condensed white conifers that seem to be in full bloom of white snow (Park Wan-hee, Korean mushroom, 2002, Kyohaksa).
동충하초는 여러 가지 질병에 대한 약리적 효능을 갖기 때문에 많은 관심과 연구의 대상이 되고 있다. 동충하초는 동의치료에서 폐와 신장을 돕고 피를 멈추며 가래를 삭인다고 하기 때문에 강장, 진정, 기침멎이로 사용되며, 또한, 몸이 허약해진 경우로 기침, 각혈, 폐결핵, 땀흘리기, 빈혈, 양위, 유정, 허리아픔에도 사용된다(약초의 성분과 이용, 1999, 일월서각).Cordyceps sinensis has been a subject of much interest and research because of its pharmacological effects on various diseases. Cordyceps sinensis is used for tonic, soothing and coughing because it helps the lungs and kidneys, stops blood and cuts phlegm in motion therapy. It is also used for coughing, bleeding, pulmonary tuberculosis, sweating, anemia, and stomach. It is also used in oil wells and back pain (components and uses of herbs, 1999, January writing).
본 발명자는 식용 식물 추출물 중 카뎁신 K의 활성을 억제하는 추출물에 대해 연구하는 과정에서 눈꽃 동충하초의 열수 및 에탄올 추출물이 카뎁신 K를 강하게 억제하는 것을 발견하였다.The present inventors found that the hot water and ethanol extracts of Snow Cordyceps sinensis strongly inhibited Kadipsin K in the course of studying the extracts that inhibit the activity of Kadipsin K in the edible plant extracts.
본 발명의 눈꽃 동충하초 추출물 제조방법으로는 공지의 방법을 사용하는 것이 가능하다. 즉, 눈꽃 동충하초만을 채취하여 그대로 또는 건조하여 잘게 자른 후 물, 에탄올, 메탄올 등의 극성 용매 및 에테르, 헥산 등의 비극성 용매를 가하여 추출할 수 있지만, 극성 용매를 사용하는 것이 바람직하다. It is possible to use a well-known method as a method for producing snow Cordyceps sinensis extract of the present invention. That is, only snowflake Cordyceps sinensis can be taken and dried as it is, or finely chopped, and then extracted by adding a polar solvent such as water, ethanol and methanol, and a nonpolar solvent such as ether and hexane, but it is preferable to use a polar solvent.
본 발명의 추출물은 일반 식품과 건강보조식품에 첨가하여 사용될 수 있으며, 약제학적 분야에서 공지된 방법에 의해 제조하여 약제학적 제제로 제형화하여 이용할 수도 있다. 또한, 필요에 따라 눈꽃 동충하초 추출물 이외에 골다공증을 예방 또는 치료하는데 사용하는 공지물질 또는 새로운 물질을 추가로 포함하여 상승작용을 발휘하도록 할 수도 있다.
Extract of the present invention can be used in addition to general foods and dietary supplements, it may be prepared by a method known in the pharmaceutical field and formulated into pharmaceutical preparations. In addition, if necessary, in addition to the extract of Cordyceps sinensis, known substances or new substances used for preventing or treating osteoporosis may be further included to exert synergism.
이하 실시예를 통하여 본 발명을 상세하게 설명한다. 그러나, 이들 실시예는 예시적인 목적일 뿐 본 발명이 이에 한정되는 것은 아니다.
The present invention will be described in detail through the following examples. However, these examples are for illustrative purposes only and the present invention is not limited thereto.
[실시예1] 눈꽃 동충하초로부터 열수 추출물 제조Example 1 Preparation of Hot Water Extract from Snow Cordyceps sinensis
우리나라에서 채집한 눈꽃 동충하초를 50g씩 파쇄하여 취하고 증류수 500ml를 첨가하고, 100℃에서 4시간 추출(뚜껑을 덮고 중탕하였으므로 4시간 가열후에 남은 용액도 처음과 거의 동일)한 후, 원심분리하여 상등액을 얻었다.
50g of snowflake cordyceps collected from Korea were crushed, and 500ml of distilled water was added and extracted at 100 ° C for 4 hours (the solution remaining after heating for 4 hours was almost the same as the first time after heating for 4 hours), followed by centrifugation. Got it.
[실시예2] 눈꽃 동충하초로부터 에탄올 추출물 제조Example 2 Preparation of Ethanol Extract from Snow Cordyceps Sinensis
우리나라에서 채집한 눈꽃 동충하초를 50g씩 파쇄하여 취하고 에탄올 500ml를 첨가하고, 60℃에서 15시간 추출(뚜껑을 덮고 중탕하였으므로 15시간 가열후에 남은 용액도 처음과 거의 동일)한 후, 원심분리하여 상등액을 얻었다.
Take 50g of snowflake cordyceps collected from Korea, add 500ml of ethanol, extract it for 15 hours at 60 ℃ (the solution remaining after heating for 15 hours is almost the same as the first time after heating for 15 hours), and then centrifuge the supernatant. Got it.
[실시예3] 눈꽃 동충하초의 열수 추출물의 농도에 따른 카뎁신 K 저해 활성 Example 3 Kadepsin K Inhibitory Activity According to the Concentration of Hot Water Extract of Snow Cordyceps Sinensis
실시예1에서 얻는 동충하초의 열수 추출물을 동결건조한 후, 증류수에 농도별로 다시 녹인 용액을 시료 용액으로 사용하였다. 카뎁신 K의 활성은 형광성 기질인 Z-Leu-Arg-AMC(Carbobenzoxy-L-Leucyl-L- Arginine-4-Methyl-Coumaryl-7-Amide)를 pH5.5인 50mM MES(2-[N-Morpholino]ethane -sulfonic acid), 2.5mM 에틸렌디아민테트라아세트산(EDTA), 10%디메틸설포옥사이드(dimethyl sulfoide, DMSO)가 함유된 완충용액에 10μM이 되도록 녹인 후, 96 well plate에 200㎕ 첨가하고, 시료 용액 20㎕와 카뎁신 K 효소액 20㎕를 첨가한 후, 25℃에서 10분간 반응시켰다. Synergy HT(Bio-Tek산, Fluorescence, Absorbance, Luminescence 측정 가능한 기기)를 사용하여 여기(excitation)파장과 방사(emission)파장이 각각 360/40nm, 460/40nm인 조건에서 생성되는 형광을 측정한 결과, 표1 및 도2와 같이 나타났다. 표1 및 도2을 참고하면, 눈꽃 동충하초 5mg/ml의 농도에서 카뎁신 K는 거의 완전히 억제되는 것으로 나타났으며, 0.5mg/ml의 농도에서 약 60%정도로 억제되는 것으로 나타났다.After freeze-drying the hot water extract of Cordyceps sinensis obtained in Example 1, the solution was re-dissolved in distilled water for each concentration was used as a sample solution. The activity of cardioxin K was determined by using a fluorescent substrate, Z-Leu-Arg-AMC (Carbobenzoxy-L-Leucyl-L-Arginine-4-Methyl-Coumaryl-7-Amide), which had a pH of 5.5 mM MES (2- [N- Morpholino] ethane -sulfonic acid), 2.5mM ethylenediaminetetraacetic acid (EDTA), 10% dimethyl sulfoide (DMSO) dissolved in a buffer solution containing 10μM, 200μL was added to a 96 well plate, After 20 µl of the sample solution and 20 µl of the capidine K enzyme solution were added, the mixture was reacted at 25 ° C for 10 minutes. Synergy HT (Bio-Tek Acid, Fluorescence, Absorbance, Luminescence) can be used to measure the fluorescence generated under the conditions of excitation and emission wavelengths of 360 / 40nm and 460 / 40nm, respectively. , Table 1 and shown in FIG. Referring to Table 1 and FIG. 2, it was found that kadipsin K was almost completely suppressed at the concentration of 5 mg / ml of the snow cordyceps, and about 60% at the concentration of 0.5 mg / ml.
<표 1> 눈꽃 동충하초 열수 추출물의 농도에 따른 카뎁신 K의 활성량(460/40nm에서의 형광) <Table 1> Activity of Kadipsin K (fluorescence at 460 / 40nm) according to the concentration of Snow Cordyceps Sinensis Extract
단위 : FUUnit: FU
[실시예4] 눈꽃 동충하초의 추출방법에 따른 카뎁신 K 저해 활성 측정Example 4 Determination of the Kadepsin K Inhibitory Activity According to the Extraction Method of Snow Cordyceps Sinensis
실시예1와 실시예2에서 얻은 상등액을 동결건조한 후, 0.5mg/ml의 농도가 되도록 증류수로 다시 녹인 후 시료 용액으로 사용하였다. 카뎁신 K의 억제활성의 측정은 실시예3과 동일하게 실시하였다. 그 결과 카뎁신 K의 저해 활성은 표2 및 도2와 같이 나타났으며, 열수 추출물과 에탄올 추출물의 억제활성이 거의 비슷였으나, 에탄올 추출물의 억제활성이 좀 더 높았다.After freeze-drying the supernatant obtained in Examples 1 and 2, it was dissolved again in distilled water to a concentration of 0.5mg / ml and used as a sample solution. Measurement of the inhibitory activity of kadipsin K was carried out in the same manner as in Example 3. As a result, the inhibitory activity of kadipsin K was shown in Table 2 and Figure 2, the inhibitory activity of the hot water extract and ethanol extract was almost the same, but the inhibitory activity of the ethanol extract was higher.
<표 2> 눈꽃 동충하초의 추출방법에 따른 카뎁신 K 활성량 (460/40nm에서의 형광) <Table 2> Kadipsin K Activity according to the Extraction Method of Snowflake Cordyceps (Fluorescence at 460 / 40nm)
단위:FUUnit: FU
[실시예5] 눈꽃 동충하초 열수 추출물의 정제 분획물의 카뎁신 K 저해 활성Example 5 Kadicecin K Inhibitory Activity of Purified Fraction of Snow Cordyceps Sinensis Extract
실시예1에서 얻은 눈꽃 동충하초 열수 추출물의 상등액을 음이온 교환 수지인 Q-200 컬럼(Bio-Rad사)을 사용하여 정제한 후, 각각의 분획물에 대하여 카뎁신 K 저해 활성을 측정하였다(도3참조). 이때 이동상(移動相)으로는 50mM Tris·HCl완충용액(pH 7.5)과 1M NaCl이 첨가된 50mM Tris·HCl완충용액(pH 7.5)을 사용하였으며, 유속 3ml/min인 조건에서 정제를 실시하였다. 그 실험 결과, 도3의 그래프 중 점선으로 그려진 그래프에서 볼 수 있듯이, 시료를 주입한 후로부터 약 14.5분에서 활성억제률은 약 90%로 최대가 되었고. 또한, 도3의 그래프 중에 실선으로 그려진 그래프에서 볼 수 있듯이, 시료를 주입한 후로부터 약 27분에 OD214nm는 약 1.7로 최대가 되었다. 따라서, Q-200 컬럼(Bio-Rad사)을 이용하여 상기와 같은 조건으로 본 발명에 따른 눈꽃 동충하초 추출물을 정제할 때는, 시료주입 후 14분 ~ 15분 사 이에 정제된 추출물에 카뎁신 K의 활성을 억제하는 유효성분이 가장 많음을 알 수 있었고, 또한, 시료주입 후 약 27분에서는 OD214nm가 최대로 되지만 카뎁신 K의 억제활성률이 높지 않으므로, 카텝신 K의 활성을 억제하는 유효성분은 214nm의 자외선에서 흡광하지 않는 것으로 추측할 수 있다.
After purifying the supernatant of the snow Cordyceps sinensis hot water extract obtained in Example 1 using a Q-200 column (Bio-Rad) which is an anion exchange resin, the capsidsin K inhibitory activity of each fraction was measured (see FIG. 3). ). At this time, 50mM Tris-HCl buffer solution (pH 7.5) and 50mM Tris-HCl buffer solution (pH 7.5) to which 1M NaCl was added were used as a mobile phase, and purification was carried out under the condition of a flow rate of 3ml / min. As a result of the experiment, as shown in the graph drawn by the dotted line in the graph of Figure 3, the activity inhibition rate was maximized to about 90% at about 14.5 minutes after the sample was injected. As shown in the graph drawn by the solid line in the graph of Fig. 3, the OD 214 nm reached a maximum of about 1.7 at about 27 minutes after the sample was injected. Therefore, when purifying the snow Cordyceps sinensis extract according to the present invention using the Q-200 column (Bio-Rad Co., Ltd.) under the same conditions as above, 14 minutes to 15 minutes after sample injection, the purified extract of kadipsin K was to find out effective component the plenty of inhibiting activity, and, after a sample injection of about 27 minutes from the active ingredient to OD 214nm is a maximum, but because the inhibition rate of the car depsin K high, suppression of cathepsin K activity It can be assumed that it does not absorb in 214 nm ultraviolet-ray.
이상과 같이 본 발명에 의하면, 눈꽃 동충하초 추출물은 카뎁신 K 효소 활성을 효과적으로 억제하는 효과가 있으며, 상기 눈꽃 동충하초 추출물의 유효성분을 포함하여 약 또는 기능성식품을 만들면 골다공증 예방 및 치료에 효과적으로 이용할 수 있다. 특히, 칼슘제제에 상기 눈꽃 동충하초 추출물의 유효성분을 추가하는 경우 골다공증 예방 및 치료를 위한 의약품을 만들 수 있다.
According to the present invention as described above, snow gingiva Cordyceps sinensis extract has an effect of effectively inhibiting the activity of the Kadepsin K enzyme, and can be effectively used for the prevention and treatment of osteoporosis by making a medicine or functional food containing the active ingredient of the snow gingiva Cordyceps sinensis extract. . In particular, when adding the active ingredient of the snowflake Cordyceps sinensis extract to the calcium preparation can make a medicine for the prevention and treatment of osteoporosis.
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| JP2004315454A (en) | 2003-04-17 | 2004-11-11 | Yamato Scient Co Ltd | Osteoporosis therapeutic agent and osteoclast formation inhibitor |
| KR100845463B1 (en) | 2006-12-21 | 2008-07-10 | 성균관대학교산학협력단 | Method for producing mushroom extract, mushroom extract obtained thereby and osteoporosis prevention and treatment agent using the same |
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| JP2004315454A (en) | 2003-04-17 | 2004-11-11 | Yamato Scient Co Ltd | Osteoporosis therapeutic agent and osteoclast formation inhibitor |
| KR100845463B1 (en) | 2006-12-21 | 2008-07-10 | 성균관대학교산학협력단 | Method for producing mushroom extract, mushroom extract obtained thereby and osteoporosis prevention and treatment agent using the same |
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