JPH0867666A - Carotenoid-containing powder formulation and method for producing the same - Google Patents

Abstract

(57) [Summary] [Objective] To provide a palm oil-extracted carotenoid-containing powder preparation excellent in storage stability without the risk of carotenoid decomposition. This preparation is useful as a colorant for foods and beverages, cosmetics, feeds, etc., a strengthening agent, and a drug such as provitamin A. [Composition] An oil-soluble antioxidant is added to carotenoids extracted from palm oil, and the mixture is heated and dissolved at 40 to 170 ° C. (preferably 100 to 150 ° C.) for about 5 to 30 minutes together with edible fats and oils. 0.5-8% by weight of anti-oxidant
And the water-soluble antioxidant / oil-soluble antioxidant is 1/1
The average particle diameter of the powder is 10 to 200 by spray drying or the like.
A powder formulation containing palm oil extracted from carotenoid having excellent storage stability of carotenoid is obtained by forming a powder of μm.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a carotenoid-containing powder preparation useful as a coloring agent and nutritional enhancer for foods and drinks, pharmaceuticals, quasi drugs, cosmetics, feeds and the like, and a method for producing the same.

[0002]

BACKGROUND OF THE INVENTION Carotenoid is a carotenoid for eliminating singlet oxygen, scavenging free radicals, preventing harmful effects of ultraviolet rays, and provitamin A.
It has been widely used recently as a physiologically active substance having actions and immunity enhancing effects, as a coloring agent for foods and drinks, pharmaceuticals, cosmetics, feeds, etc., and as a raw material for health foods, which is expected to have an antioxidant effect on carotenoids. However, carotenoids are hardly soluble in water, alcohol, etc., and have a relatively high melting point, and their solubility in solvents such as fats and oils is low,
In addition, since it is a substance that is easily oxidized, many proposals have been made regarding a solvent capable of dissolving carotenoids at a higher concentration than in the past, and a production method capable of obtaining stable carotenoid emulsions and dry powders. .

[0003] For example, there is a method for producing a water-dispersible carotenoid preparation which is obtained by supersaturating a carotenoid in edible oil and emulsifying it in an aqueous gelatinous substance to obtain an emulsion (Japanese Patent Publication No. 35-8095). Further, although basically similar to this proposal, as a solvent that replaces the above edible oil, for example, flavor oils such as orange oil, lemon oil, and rose oil, terpenes such as limonene, yonon, and menthone, curcuma oil, and the like. Terpene-containing oils (No. 36-21476), essential oils and / or vegetable oils (No. 37-8532), volatile water-insoluble solvents such as chloroform, methylene chloride, carbon tetrachloride (No. 37-12428) , Acetoglyceride (No. 39-21760), aromatic aldehyde, aromatic alcohol or its derivative (No. 40-18018), castor oil or dehydrated lanolin (No. 40-2450).
No. 8), monoesters and diesters of higher fatty acids having 10 or more carbon atoms and propylene glycol (Id.
6980), hydrogenated castor oil polyoxyethylene ether (degree of polymerization of oxyethylene 60-100).
(Ibid. 44-6986), hydrogenated limonene dimer (D. 45-9220), orange oil or lemon oil (D. 45-24379), vegetable oil, mineral oil,
At least one kind of essential oil and natural rubber (48-136)
No. 87), a triglyceride of a saturated fatty acid having 8 to 10 carbon atoms (No. 52-12207), a homogenate 7 consisting of 1 part of a natural essential oil and 0.01 to 500 parts of a vegetable oil.
To 12 parts (Japanese Patent No. 54-28858), squalene,
Fats and oils containing squalane or squalene (ibid.
431), vitamin E (JP-A-60-10216).
No. 9) has been proposed, and an aqueous protective colloid may be added to the carrier oil (62-240).
No. 364), a carotenoid derived from seaweed (No. 64-43167), or rosin is added to the oily composition (Japanese Patent Laid-Open No. 5-38273), edible fats and oils and emulsifiers on palm oil carotene. Techniques such as adding a dispersant (Japanese Patent Laid-Open No. 6-172170) have also been proposed.

However, the above-mentioned conventional water-soluble carotenoid powder preparations are liable to be discolored or deteriorated by light or heat.

The present invention has been made in view of the above circumstances, and is very stable, has a high residual rate of carotenoid even after long-term storage, and has no problem in taste, smell, etc. even when eaten or eaten. An object of the present invention is to provide a powder preparation and a method for producing the same.

[0006]

MEANS TO SOLVE THE PROBLEMS As a result of intensive studies for achieving the above-mentioned object, the present inventor has found that especially carotenoids are mixed with fats and oils, and the oil phase containing an oil-soluble antioxidant is water-soluble. Emulsifying in an aqueous phase containing antioxidants,
In this case, the water-soluble antioxidant is 0.5 to 8% by weight, and the water-soluble antioxidant / oil-soluble antioxidant is 1/1 in weight ratio.
The average particle size of the oil phase in the emulsion is 100 to 500 nm, and the emulsion is dried and powdered to have an average particle size of 10 to 2
It was found that the powder of 00 μm suppresses the decomposition of carotenoid even when it is stored for a long period of time or added to foods, etc., and a very stable carotenoid-containing preparation can be obtained.

That is, in the carotenoid powder formulation, the present inventor adjusted the water-soluble antioxidant to the above specified optimum range in addition to the carotenoid and the oil and fat in each powder, and specified the water-soluble and oil-soluble antioxidants as described above. Inclusion in a ratio,
By adjusting the average particle size of the emulsion when producing a powder formulation to the above specific optimum range, and by setting the average particle size of the powder formulation obtained by dry powdering this to the above specific optimum range, carotenoids are significantly Stabilization, in contrast, when the water-soluble antioxidant and the oil-soluble antioxidant are not used in combination, when the ratio is out of the above range even when they are used in combination, the average particle size of the powder formulation is further out of the range. in case of,
It was discovered that stabilization of carotenoids was not achieved.

Therefore, the present invention comprises (1) a carotenoid, fats and oils, a water-soluble antioxidant and an oil-soluble antioxidant, wherein the water-soluble antioxidant is 0.5 to 8% by weight. The weight ratio of water-soluble antioxidant and oil-soluble antioxidant is 1: 1.
˜1: 10 and average particle size 10 to 200 μm
A carotenoid-containing powder preparation, which is characterized by comprising the powder of 1., and (2) the above-mentioned preparation for food and drink.

The present invention will be described in more detail below. As described above, the carotenoid-containing powder preparation of the present invention is a powder containing a carotenoid, an oil and fat, a water-soluble antioxidant and an oil-soluble antioxidant. It consists of Further, this preparation is usually obtained by dry-powdering an emulsion obtained by emulsifying an oil phase containing a carotenoid, a fat and oil, and an oil-soluble antioxidant into an aqueous phase containing a water-soluble antioxidant, Since an emulsifier or dispersant is generally used when emulsifying into an aqueous phase, an emulsifier or dispersant is usually contained in each powder of the powder preparation.

Here, the carotenoid is not particularly limited, but carotenoid obtained by extracting palm oil is preferably used. This palm oil extracted carotenoid generally has the following composition. β-carotene: 55-70% (% by weight, the same applies below) α-carotene: 20-40% Total amount of α-carotene and β-carotene: 85-97% Other natural carotenoids and natural pigments such as lycopene: 3-
15% (typically γ-carotene: 3-5%, lycopene: 3-
5%)

By using this carotenoid extracted from palm oil, it is possible to obtain the advantage that when added to foods, it has no offensive odor and is excellent in flavor.

The method for producing the above palm oil-extracted carotenoid is not particularly limited, but is disclosed in, for example, Japanese Patent Laid-Open No.
Carotenoids collected and concentrated from palm oil by the methods described in JP-A-1-125757, JP-A-63-5073, JP-A-63-5074, etc. are further described, for example, in JP-A-63-132871 and 63-295551.
JP-A No. 1-160953 and JP-A 1-160
Examples thereof include a method of purifying by the method described in Japanese Patent Publication No. 954, etc., and a palm oil-extracted carotenoid purified in this manner is preferable.

The fats and oils are selected according to the intended use of the powder formulation, and when used in foods and drinks, medicines, feeds, etc., edible fats and oils are used. Specifically, soybean oil, rapeseed oil,
Animal and vegetable oils such as corn oil, olive oil, coconut oil, peanut oil, safflower oil, sunflower oil, rice oil, beef tallow, lard, fish oil and processed edible oils and fats such as C _{6 to} C ₁₂ medium chain saturated fatty acid triglycerides and Any mixture of these edible oily materials may be mentioned.

The use ratio of the above carotenoid and oil is
The weight can be arbitrarily selected within the range of 5:95 to 95: 5, but it is more preferably 10:90 to 60:40 from the viewpoint of handling the carotenoid / fat mixture.

As the water-soluble antioxidant, one or more of ascorbic acid and its water-soluble salt, erythorbic acid and its water-soluble salt, catechin, enzyme-treated rutin and the like can be used.

As the oil-soluble antioxidant, tocopherols, ascorbic acid fatty acid ester, spice extract,
One kind or two or more kinds of butylhydroxyanisole, butylhydroxytoluene and the like can be used, but not limited to these, and a known antioxidant can be used.

Here, the content of the water-soluble antioxidant is 0.5 to 8%, preferably 0.5 to 5 based on the total amount of the powder preparation.
%, More preferably 0.8 to 3%, and the mixing ratio of the water-soluble antioxidant and the oil-soluble antioxidant (water-soluble antioxidant / oil-soluble antioxidant) is 1/1 to 1 / It is effective to set it to 10, preferably 1/2 to 1/8. If the content of the water-soluble antioxidant is less than 0.5%, a sufficient antioxidant effect may not be obtained, and if it exceeds 8%, the stability of the emulsion or powder formulation may be deteriorated. Further, the mixing ratio of the water-soluble antioxidant and the oil-soluble antioxidant is 1/1 to 1 /
When it is out of the range of 10, the antioxidant mechanism of the two antioxidants is not well entangled with each other, and the object of the present invention cannot be achieved.

The emulsifier and dispersant used to obtain the emulsion in the present invention are not particularly limited,
What is generally used as an emulsifier and a dispersant can be used, but for example, gelatin, pectin, gum arabic, polyvinylpyrrolidone, tragacanth gum, agar, carboxymethyl cellulose (CMC), dextrin, polyvinyl alcohol, starch, starch succinate. Chemically modified starch such as, methyl cellulose, alginate, sugar, lactose, glucose, saccharides such as fructose and the like are preferable.

The amount of these emulsifiers and dispersants used is such an amount that the oil phase is emulsified in the water phase, and is usually 2-1 to the weight of the oil phase.
It is 0 times the amount.

As a method for obtaining the carotenoid-containing powder preparation of the present invention, first, an oil phase containing the above carotenoid, fats and oils and an oil-soluble antioxidant is emulsified into an aqueous phase containing a water-soluble antioxidant to form an O / W type. Prepare the emulsion.

In this case, the oil phase can be prepared by mixing the carotenoid with the fat and oil, dissolving or suspending it, and adding an oil-soluble antioxidant. In particular, a suspension in which carotenoid is suspended in the above fats and oils is prepared under stirring conditions at about 40 to 160 ° C., preferably 100 to 1
By heating at 40 ° C. for 5 to 30 minutes, the carotenoid can be completely dissolved. At this time, it is preferable to perform it in an atmosphere of any inert gas such as helium, argon, nitrogen or carbon dioxide. Further, the above-mentioned oil-soluble antioxidant can be added when the suspension is heated.

The aqueous phase can be prepared by dissolving a water-soluble antioxidant, an emulsifier and a dispersant in water.

When the oil phase is emulsified in the water phase to obtain an O / W type emulsion (emulsion), the oil phase and the water phase can be used in an appropriate ratio, and the weight ratio is 95: 5 to 5. : 9
It can be used in a ratio of 5, especially 20:80 to 5:95. This mixing ratio can be appropriately selected to obtain the desired carotenoid content.

In the production method of the present invention, when the oil phase is emulsified into the aqueous phase, it is emulsified by a known means using a usual stirring emulsifying machine, high pressure homogenizer, ultrasonic emulsifying machine, ultra mixer, colloid mill or the like. Can be processed.

In this case, in the manufacturing method of the present invention,
It is necessary that the average particle size of the oil phase (oil particles) is 100 to 500 nm, particularly 150 to 300 nm. If the average particle size is smaller than 100 nm, the color tone becomes transparent and the desired color tone cannot be obtained. When it is larger than 500 nm, sufficient stability of carotenoid cannot be obtained.

As a method for adjusting the average particle size of the carotenoid-containing emulsion to 100 to 500 nm,
A known method for adjusting the particle size of an emulsion can be adopted, such as adjusting the emulsification conditions such as stirring speed and stirring time, and adjusting the addition amounts of the emulsifier and the dispersant.

Next, in the present invention, the above emulsion is dried and powdered. As this method, for example, a vacuum drying method,
It can be pulverized by any method such as a spray drying method and a fluidized bed drying method, but the spray drying method is particularly preferable.

The carotenoid-containing powder preparation of the present invention thus obtained must have an average particle size of 10 to 200 μm, preferably 20 to 150 μm, more preferably 40 to 100 μm. If it is less than 10 μm or exceeds 200 μm, sufficient stability of the powder formulation cannot be obtained.

The carotenoid-containing powder preparations of the present invention include frozen desserts, noodles, confectionery, protein foods, processed fats and oils, health foods, in addition to pharmaceuticals, quasi drugs, cosmetics, feeds, etc.
It can be added as a coloring agent for foods and drinks such as seasonings and a nutritional enhancer. More specifically, the food and drink described above,
Examples of frozen desserts include ice cream, sorbet, shaved ice and the like. As the noodles, buckwheat, udon, harusame, dumpling skin, dumpling skin and other wheat flour, buckwheat flour, raw noodles made by adding water to starch, dried noodles, boiled noodles, Chinese noodles, steamed noodles, Examples thereof include noodles and instant noodles. Examples of confectionery include hard candy, sand cream, jam, jelly, cream, baked confectionery, and the like, and as proteinaceous foods,
Kamaboko, ham, sausage, other processed livestock products,
Milk, fermented milk, other dairy products, processed oils and fats, margarine, mayonnaise, shortening, whipped cream, dressing, bread, etc., healthy foods, soft capsules, tablets, various other dosage forms, etc. Examples of the seasoning include sauce, sauce, soup and the like.

[0030]

The carotenoid-containing powder preparation of the present invention comprises:
It is a very stable formulation that does not easily discolor or deteriorate even when exposed to light or heat, and since it has no problem with taste and smell when eaten and drink, it has a singlet oxygen scavenging action, a free radical scavenging action, and an ultraviolet ray. It is extremely effective as a preparation containing physiologically active substances such as prevention against harmful effects of, provitamin A effect, immunity-enhancing effect, etc., and as a coloring agent for food and drink, cosmetics, feed, etc. .

According to the production method of the present invention, such a carotenoid-containing powder preparation can be produced easily and reliably.

[0032]

EXAMPLES The present invention will be specifically described below by showing Examples and Comparative Examples, but the present invention is not limited to the following Examples. In the following examples,% means% by weight.

[Example 1] Palm oil extracted carotenoid was prepared into a 30% suspension with vegetable oil, and 30 g of this suspension was prepared.
And 12 g of natural tocopherol under nitrogen stream 140
The mixture was heated at 5 ° C for 5 minutes to obtain an oily solution in which the carotenoid was uniformly dissolved. The oily solution was cooled to about 80 ° C.
3 g of ascorbic acid, 30 g of saccharose and 50% processed starch (trade name: Capsules; made by National Starch)
Aqueous solution obtained by mixing 420 g of the aqueous solution and 20 g of water was mixed with 80
The oily solution was poured into the aqueous solution while being heated to ℃, and the aqueous solution was stirred, and emulsified with a TK homogenizer at 10,000 rpm for 10 minutes to obtain an emulsion (ascorbic acid / tocopherol). = 1/4).

Next, the above emulsion was spray-dried using a rotary disc spray dryer (manufactured by Okawara Kakoki) at a disc rotation speed of 20000 rpm, a hot air inlet temperature of 150 ° C., and an exhaust air temperature of 80 ° C. to obtain a palm oil-extracted carotenoid-containing powder. 280 g of formulation was obtained. The carotenoid content in this preparation was quantified by the following method in accordance with "[beta] -carotene purity test (Food Additive Official Specification 5th Edition, p.187, 1986)".

Method for quantitatively determining carotenoid amount About 100 mg of a sample is precisely weighed and diluted with distilled water to make exactly 100 ml. Accurately measure 5 ml of this diluted solution, and add acetone to make exactly 100 ml. This solution was filtered through non-aqueous Millipore (0.5 μm), the filtrate was measured for absorbance at the maximum absorption wavelength near a wavelength of 450 nm using a spectrophotometer, and the carotenoid content was determined by the following formula.

[0036]

[Equation 1]

As a result of quantification by the above-mentioned method, the carotenoid content in the preparation obtained in this Example was 3.
It was 27%.

The average particle size of the emulsion in this example was 250 nm as a result of measurement with a super dynamic light scattering photometer DLS-700 manufactured by Otsuka Electronics Co., Ltd.
Met. The average particle size of the powder preparation in this example was 50 μm as a result of measurement with a microscope.

Comparative Example 1 Similar to Example 1 except that the rotation speed of the TK homogenizer during emulsification was 5000 rpm and the disk rotation speed of the spray dryer during dry powdering was 5000 rpm. Then, the same amount of carotenoid-containing powder preparation as in Example 1 was obtained. When the carotenoid content was quantified in the same manner as in Example 1, the carotenoid content in the obtained preparation was 3.25%. Moreover, as a result of measuring the average particle diameter in the same manner as in Example 1, the average particle diameter of the emulsion was 600 n.
The average particle diameter of the powder preparation was 300 μm.

Comparative Example 2 In Example 1, the amount of natural tocopherol added was 0.3 g and the amount of ascorbic acid added was 1 g (ascorbic acid / tocopherol = 3 /
272 g of a carotenoid-containing powder preparation was obtained in the same manner as in Example 1 except for 1). For this powder formulation,
When the carotenoid content was quantified in the same manner as in Example 1, the carotenoid content in the obtained preparation was 3.18.
%Met. Further, the average particle size was measured in the same manner as in Example 1, and as a result, the average particle size of the emulsion was 250 nm and the average particle size of the powder preparation was 50 μm.

The following experiments were carried out on the powder formulations obtained in the above Examples and Comparative Examples. Experiment 1 After filling each retort pouch with each powder formulation, 40 ° C
It was stored in an incubator for 1 month, and after 1 month and 6 months, the residual rate of carotenoid in each preparation was measured. The results are shown in Table 1.

[0042]

[Table 1]

From the results shown in Table 1, it was confirmed that the powder formulation according to the present invention was extremely stable even after storage at 40 ° C. for 6 months with almost no decomposition of carotenoid, and the storage stability of carotenoid was improved. To be On the other hand, when the average particle size of the emulsion and powder is outside the range of the present invention (Comparative Example 1), or the ratio of the water-soluble antioxidant to the oil-soluble antioxidant and the weight of the water-soluble antioxidant. When% is outside the range of the present invention (Comparative Example 2), it is recognized that the storage stability is poor.

Experiment 2 Next, citric acid 0.2%, sodium citrate 0.02
%, L-ascorbic acid was added to 0.2% in an aqueous solution, or an acid solution without any additive was prepared, and the formulations of Examples or Comparative Examples were prepared to have a concentration of 0.1%. After filling these in a transparent glass bottle, sterilize,
A sample solution was used. One week after each was exposed to sunlight, the carotenoid residual rate of each sample solution was measured. Table 2 shows the results
Shown in

[0045]

[Table 2]

From the results shown in Table 2, the carotenoid contained in the powder preparation of the present invention is extremely stable even when stored as an acidic solution under exposure to sunlight, and the storage stability of the carotenoid in the powder preparation of the present invention is extremely high. On the other hand, the carotenoids contained in the powder formulations of Comparative Examples 1 and 2 are extremely unstable, and it is recognized that the carotenoids hardly remain particularly after 2 weeks in the case of using the ascorbic acid-added solution.

Experiment 3 In Experiment 2, the same experiment as in Experiment 2 was carried out except that each sample solution was stored in a constant temperature bath at 35 ° C. and the carotenoid residual rate in each sample solution was measured after 2 weeks. The results are shown in Table 3.

[0048]

[Table 3]

From the results shown in Table 3, it is recognized that the carotenoid contained in the powder preparation according to the present invention is particularly stable when stored as an ascorbic acid-added solution.

Experiment 4 McIlvain at pH 3, 5, and 7 with or without addition of L-ascorbic acid to a final concentration of 0.2%.
In a buffer solution, each carotenoid-containing powder preparation of Example or Comparative Example was dissolved to a concentration of 0.1% was filled in a glass bottle, which was then sealed and sterilized to obtain a sample solution. Each sample solution was stored in a constant temperature bath at 35 ° C., and two weeks later, the carotenoid residual rate in each sample solution was measured.
The results are shown in Table 4.

[0051]

[Table 4]

From the results shown in Table 4, the powder preparation according to the present invention has a p value when ascorbic acid is added or not added.
It is recognized that the storage stability of carotenoid is improved as compared with the comparative example in all cases where the solutions of H3, 5, and 7 were used.

Experiment 5 In Experiment 3, all sample solutions were L-ascorbic acid-added acid solutions, and sodium chloride was added at 0%, 2.5%, 10%.
%, 15% solution was added, and the same experiment as in Experiment 3 was performed. The results are shown in Table 5.

[0054]

[Table 5]

From the results shown in Table 5, it is recognized that the powder preparation according to the present invention has excellent carotenoid storage stability even under the condition that salt is added.

Experiment 6 Each of the preparations of Examples and Comparative Examples was added to ion-exchanged water to which L-ascorbic acid was added so as to be 0.2% or not to be added so as to have a concentration of 0.1% solution. After dissolution, each solution was filled in a pressure-resistant heat-resistant bottle, heated at 121 ° C. for 1 hour, and the carotenoid residual rate in each solution was measured. The results are shown in Table 6.

[0057]

[Table 6]

From the results shown in Table 6, it is recognized that the carotenoid contained in the powder preparation of the present invention has improved stability under the above conditions.

Experiment 7 15 parts of sugar, 13 parts of whole milk powder, 7 parts of unsalted butter, 5 parts of powdered starch syrup, 0.3 part of stabilizer, 0.1 part of food flavor, carotenoid-containing powder preparation of Examples or Comparative Examples An ice cream mix was prepared by mixing 0.01 part (containing 3% carotenoid) and 59.5 parts of water. After each ice cream mix was sterilized and cooled, it was put into a soft cream maker, taken out at 80% overrun, filled in a cup and then frozen to obtain an ice cream. Each was left at −20 ° C. for 6 months and the residual rate of carotenoid was measured. The results are shown in Table 7.
Shown in

[0060]

[Table 7]

Experiment 8 100 parts of semolina and 0.02 part of the carotenoid-containing powder formulation (containing 3% carotenoid) of the Examples or Comparative Examples were mixed well, and water was added so that the water content was 31%.
Mix for 10-20 minutes at 40 ° C to make dough. Pass each dough through high pressure kneading, remove air bubbles, then extrude, cut,
It was dried to obtain spaghetti. 1 at 25 ° C each
It was stored for a year and the residual rate of carotenoid was measured. Table 8 shows the results.

[0062]

[Table 8]

Experiment 9 235 parts of granulated sugar, 290 parts of starch syrup (75%), 1 part of water
80 parts, pectin 9.7 parts, stabilizer 1.8 parts, citric acid 2 parts, sodium citrate 0.75 parts were heated and dissolved, and the carotenoid-containing powder preparation (containing 3% carotenoid) of Example or Comparative Example 0 After addition of 0.07 part (pH 3.
8) Then, each was poured into a mold and allowed to stand overnight to prepare pectin jelly. Each was preserve | saved for 6 months at 5 degreeC, and the residual rate of the carotenoid was measured. The results are shown in Table 9.

[0064]

[Table 9]

Experiment 10 To 60 parts of unsalted margarine, 30 parts of upper sucrose and 50 parts of whole egg were added for creaming, and then 22.5 parts of water was added little by little, and further creaming was performed to prepare a cream part. 100 parts soft flour, 0.8 parts baking powder and the carotenoid-containing powder formulation of the Example or Comparative Example (3%
A mixture of 0.5 parts of carotenoid (containing carotenoid) in advance was added to each of the above cream parts and mixed well, and each was molded and baked at 160 ° C. for 25 minutes to obtain a cookie. Each cookie was stored at 25 ° C. for 1 year, and the residual rate of carotenoid was measured. The results are shown in Table 10.

[0066]

[Table 10]

Experiment 11 1.5 parts of salt, 0.3 parts of MSG, white pepper 0.
2 parts, liquid sugar 10 parts, vinegar 25 parts, soy sauce 30 parts, water 7.8
Parts, carotenoid-containing powder formulations of Examples or Comparative Examples (3
% Of carotenoids) and 25 parts of corn salad oil were mixed to obtain soy sauce dressings. Each was preserve | saved for 12 months in a 25 degreeC thermostat, and the residual rate of the carotenoid was measured. The results are shown in Table 11.

[0068]

[Table 11]

Experiment 12 To 90 parts of milk was added 10 parts of sugar, 0.1 part of flavor, 0.02 part of the carotenoid-containing powder preparation (containing 3% carotenoid) of the Examples or Comparative Examples, and sterilized at 90 ° C. for 20 minutes. After cooling, lactic acid bacteria were added and fermented at 35 ° C. for 2 days to obtain yogurt. Each was preserve | saved for 6 months at 5 degreeC, and the residual rate of the carotenoid was measured. The results are shown in Table 12
Shown in

[0070]

[Table 12]

Experiment 13 Reduced lactose 57.8 parts, honey powder 10 parts, carotenoid-containing powder formulation (containing 3% carotenoid) of Example or Comparative Example 5 parts, tocopherol powder 15 parts, ascorbic acid 10 parts, powder fragrance 1. 5 parts and 0.7 part of an emulsifier were mixed, respectively, and tablets were obtained by a reverse arm type of φ18 mm. Each was preserve | saved at 25 degreeC for 1 year, and the residual rate of the carotenoid was measured. The results are shown in Table 13.

[0072]

[Table 13]

Experiment 14 Granulated sugar 44 parts, salt 17 parts, tomato powder 14
Parts, wheat flour 8 parts, powder soy sauce 4 parts, MSG 4 parts, HAP powder 3 parts, malic acid 1 part, black pepper 1 part, garlic powder 1 part, carotenoid-containing powder preparation of Example or Comparative Example (containing 3% carotenoid) 3 parts were mixed respectively to obtain a powder seasoning for spaghetti. 2 each
It preserve | saved at 5 degreeC for 1 year, and measured the residual rate of the carotenoid. The results are shown in Table 14.

[0074]

[Table 14]

From the results of Tables 7 to 14, when the carotenoid-containing powder preparation of the present invention was used by adding it to frozen desserts, noodles, confectionery, processed oil and fat foods, protein foods, health foods and seasonings. It is recognized that the storage stability of carotenoid in the food composition is also very excellent.

Experiment 15 Beeswax 3 parts, Cetanol 3 parts, Paraffin wax 5
Parts, hardened beef tallow oil 10 parts, isostearyl palmitate 1
0 parts, 10 parts of squalene, 5 parts of glycerin, 3 parts of decaglycerin monolaurate, 1 part of glycerin monostearate were heated at 80 ° C. and dissolved, and then the carotenoid-containing powder preparation of Example or Comparative Example (containing 3% carotenoid) 1 part and 49 parts of water were added and emulsified to obtain a cosmetic cream. Each was stored at 25 ° C. for 1 year, and the residual rate of carotenoid was measured. The results are shown in Table 15.

[0077]

[Table 15]

Experiment 16 Ten parts of phenobarbital are weighed in a mortar and well ground. Then, 1 part of the carotenoid-containing powder preparation (containing 3% carotenoid) of Example or Comparative Example is added, and the mixture is thoroughly mixed and ground. Next, 89 parts of lactose was added little by little to obtain a light pink uniform phenobarbital powder. 1 at 25 ° C each
It was stored for a year and the residual rate of carotenoid was measured. The results are shown in Table 16.

[0079]

[Table 16]

Experiment 17 Non-fat dry milk 74.8 parts, animal fats and oils 19 parts, fish solubles 4 parts, vitamin mixture 0.5 parts, mineral mixture 0.7 parts, carotenoid-containing powder preparation of Example or Comparative Example (3% (Containing carotenoid) was mixed with 1 part to obtain a feed for cattle breeding. Each was stored at 25 ° C. for 1 year, and the residual rate of carotenoid was measured. The results are shown in Table 17.

[0081]

[Table 17]

From the results shown in Tables 15 to 17, the carotenoid-containing powder preparation of the present invention has excellent storage stability of carotenoid in the composition even when added to cosmetics, pharmaceuticals, feeds, etc. It is recognized that

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Claims (5)

[Claims] 1. A carotenoid, a fat and oil, a water-soluble antioxidant and an oil-soluble antioxidant, wherein the water-soluble antioxidant is 0.5 to 8% by weight, and the water-soluble antioxidant is used. A carotenoid-containing powder preparation characterized by comprising a powder having a weight ratio of 1: 1 to 1:10 with an oil-soluble antioxidant and an average particle diameter of 10 to 200 μm. 2. A carotenoid is obtained by extracting palm oil, and β-carotene is 55 to 70% by weight, α-carotene is 20 to 40% by weight, and the total amount of β-carotene and α-carotene is 85 to 85% by weight. The formulation according to claim 1, which is a carotenoid containing 97% by weight. 3. The preparation according to claim 1, which is for food and drink. 4. A carotenoid is mixed with fats and oils, and
An aqueous phase containing an oil phase to which an oil-soluble antioxidant is added, the water-soluble antioxidant being contained so that the weight ratio of the water-soluble antioxidant to the oil-soluble antioxidant is 1: 1 to 1:10. To obtain an O / W type emulsion having an average particle size of 100 to 500 nm, which is dried and powdered to have an average particle size of 10 to 200 μm.
A method for producing a carotenoid-containing powder formulation, which comprises obtaining a powder of m. 5. A carotenoid is obtained by extracting palm oil. 55-70% by weight of β-carotene, 20-40% by weight of α-carotene, and 85-97 as the total amount of β-carotene and α-carotene. The production method according to claim 4, which is a carotenoid containing 50% by weight.