JPH06269276A - Microbial filtration bag - Google Patents

Abstract

(57) [Abstract] [Objective] The present invention is a filtration bag used for preparing a sample, which is mainly used for examining microorganisms in a solid substance such as food or a sample containing the solid substance, and has a filtration rate of The purpose is to provide a filter bag that is fast and has good workability. [Structure] A filtration bag having a stock solution chamber and a test solution chamber in which a filtration film 12 is sandwiched between a front surface film 11 and a back surface film 13 and three sides of a vertical seal 14 and a horizontal seal 15 are sealed. A film having good tearability such as a uniaxially stretched film is used as the surface film of the filtration bag, a non-sealing portion is provided in a part of the vertical sealing portion 14, and a U-shaped notch for opening is further provided in the non-sealing portion. The test chamber is provided so that the test chamber can be easily opened.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a filter bag mainly used for preparing a sample when inspecting microorganisms in a solid substance such as food or a sample containing the solid substance.

[0002]

2. Description of the Related Art Conventionally, in order to prepare a sample for inspecting microorganisms contained in solid substances such as foods, a sample such as
Put raw fish, vegetable salad, etc. in a sterilized plastic bag, add sterilized physiological saline, seal the bag, and put it on a machine such as a stomacher to suspend the microorganisms in the sample by rubbing the sample in physiological saline. Then, the supernatant is collected with a pipette or the like to measure the number of microorganisms. In addition, by sealing a part of the inside of the bag with a filter material, it is divided into a stock solution chamber and a test solution chamber, or a filter film is attached to the side surface of the film constituting the bag to separate the stock solution chamber and the test solution chamber. It is divided into filter bags. When using the filter bag, put the sample and sterilized physiological saline in the stock solution chamber, seal the bag and apply it to a machine such as a stomacher to suspend the microorganisms in the sample by rubbing the sample and suspending the sample in physiological saline. To prepare a suspension of. The suspension is filtered by the filter material, and only the filtrate fills the test chamber. In this case, while being applied to a machine such as a stomacher, the test solution is separated from the solid matter and filled in the test solution chamber, so after stirring the sample for a certain period of time, immediately sample the test solution with a pipette or the like to measure the microorganisms. Therefore, the measurement can be performed with relatively high accuracy.

[0003]

However, since the filtration bag has the same opening in the stock solution chamber and the test solution chamber, the solution in the stock solution chamber does not spill when the test solution is transferred to another container. Therefore, it is inconvenient to handle. Further, since the filter bag is flexible and has poor workability and the opening of the test solution chamber is narrow, it is quite difficult to collect the test solution without spilling the solution in the stock solution chamber.

[0004]

[Means for Solving the Problems] A bag for preparing a sample for microbiological examination, which is made of a plastic film, and is a three-sided bag in which a film capable of filtering solid matter such as a microporous film or a non-woven fabric is sandwiched between plastic films. Was sealed to form a bag. In addition, a film with good tearability is used on at least one side of the bag, and a non-sealing part is provided on a part of the sealing part in the tearing direction of the film on this film, and opening is started in this non-sealing part. V-shaped, U-shaped, etc. cuts are provided.

Next, a more detailed description will be given with reference to the drawings. FIG. 1 is a perspective view of a filter bag according to the present invention, and FIG. 2 is a sectional view of an opening of the filter bag. Surface film 11
The filter film 12 is sandwiched between the back film 13 and the back film 13, and the vertical seal 14 and the horizontal seal 15 are provided to form a three-sided seal filter bag.

A sample for microbiological measurement is put together with sterile physiological saline in a stock solution chamber 16 composed of a back film 13 and a filtration film 12, and the opening is heat-sealed, or
Seal by sandwiching it with a clamp. Next, a microorganism such as a specimen is suspended in physiological saline by rubbing the specimen with a machine such as a stomacher to prepare a suspension of the specimen.
Since liquids and fine particles can move freely through the filtration film 12 while being applied to a machine such as a stomacher, the fine particles containing microorganisms enter the test chamber 17 sandwiched between the surface film 11 and the filtration film 12 together with the liquid. Moving. Since the liquid entering the test chamber is filtered by the filtration film, it can be directly sampled with a pipette or the like to obtain a measurement sample. By vibrating for a certain period of time, microorganisms are separated from the sample, and the concentration of microorganisms in the stock solution chamber and the test solution chamber reaches equilibrium. When the concentration of microorganisms reaches the equilibrium, stop the machine, open the opening and take a sample from the test chamber to measure the microorganisms.

3 and 4 show the case where the filter bag is provided with a notch for opening. A film having good tearability such as a uniaxially stretched film is used as the surface film 11 constituting the filtration bag shown in FIG. 3, the film for filtration 12 is sandwiched in the middle and heat-sealed together with the back film 13, and the film shown in FIG. Make a bag. A surface film 1 which is formed into a bag so that the stretching direction of the uniaxially stretched film is in the lateral direction of the bag and which constitutes the bag.
1 makes it easy to tear in the lateral direction.

When manufacturing the above-mentioned filter bag, as shown in FIG. 3, a non-sealing portion 22 is provided in a part of the heat-sealing portion, that is, the vertical sealing portion 14 in the tearing direction of the stretched film. A notch 23 such as a V-shape or a U-shape for opening the opening is provided to facilitate the opening.

As a method of using the filtration bag in the previous period, a sample and sterile physiological saline are put together in a stock solution chamber sandwiched between a back film 13 and a filtration film 12 of the filtration bag, and an opening is made together with the front film 11. Is heat-sealed, and a seal portion 24 is formed as shown in FIG. Next, a microorganism such as a specimen is suspended in physiological saline by rubbing the specimen with a machine such as a stomacher to prepare a suspension of the specimen. The test solution for microbiological test is moved to the test solution chamber 17 sandwiched between the surface film 11 using the stretched film and the filtration film 12 by vibrating for a certain period of time.

The test liquid is taken out from the U for starting the opening in FIG.
When the film 21 is torn laterally by using the character notch 23, the film 21 is torn, and as shown in FIG.
Thus, the test liquid outlet 26 is formed. The test liquid can be sampled from the outlet 26 with a pipette or the like and applied to the measurement of microorganisms. It is also possible to use this outlet and transfer it to another container. In both cases, the stock solution chamber is sealed by heat sealing, so there is no risk of the solution overflowing, and the size of the outlet can be freely adjusted as needed, so workability is good and contamination from microorganisms from the outside is also possible. The number of microorganisms becomes extremely small, and highly accurate measurement of microorganisms becomes possible.

[0011]

Since the filtration bag constructed according to the present invention has a large area for the filtration film, the filtration speed is fast and the concentration of the microorganisms in the stock solution chamber and the test solution chamber separated by the filtration film can easily reach equilibrium, and Since the test solution chamber can be easily opened in a state where the stock solution chamber is sealed, workability is improved, contamination of microorganisms from the outside is reduced, and highly accurate measurement of microorganisms is possible.

[0012]

The present invention will be described in detail below with reference to the drawings based on embodiments. A first embodiment will be described with reference to FIG. As a plastic film, a laminated film was manufactured by dry laminating a stretched nylon (hereinafter referred to as ON) film 15 μm and a low density polyethylene (hereinafter referred to as LDPE) film 60 μm. A low-density polyethylene microporous film 60 [mu] m (pore diameter 10 to 20 [mu] m) as a filtration film is sandwiched between two films having the LDPE surface inside of the above laminated film and heat sealed on three sides to show in FIG. I made a filter bag like this. FIG. 5 is a cross-sectional view of the opening of the bag, showing the film structure. The size of the bag is 300 × 180 mm, vertical,
The lateral seal width was 12 mm.

As a plastic film constituting the filter bag, a linear low density polyethylene (hereinafter referred to as L-LDP) is used.
E), polypropylene (hereinafter PP), ON
/ L-LDPE, ON / LDPE / L-LDPE, polyethylene terephthalate (hereinafter PET) / LDP
E, PET / L-LDPE, etc. can be used. When forming a bag with these films, generally, the same film is used on both sides and a filtration film is sandwiched between them to form a bag, but it is also possible to use different types of films on both sides to form a bag. it can. As the filtering material, microporous LDPE, L
-LDPE, PP, a film or sheet of ethylene / vinyl acetate copolymer or the like, a synthetic resin non-woven fabric, a fiber product or the like can be used.

In the second embodiment, a case where a stretched film is used on one side of a plastic film forming a filter bag to facilitate opening and improve workability will be described. Uniaxially stretched PP film 25μ as a tearable film
It was manufactured by laminating an LDPE film of 30 μm on m by an extrusion method. Also, a configuration of PET 12μ / uniaxially stretched PE 25μ / LDPE 30μ can be used. Next, the film and the L-LDPE film 60μ
A microporous LDPE film 60 [mu] m (pore size 10 to 20 [mu] m) as a filtration film was sandwiched between m and heat-sealed to produce a filtration bag. The bag size was the same as in the first embodiment.

As shown in FIG. 6, a uniaxially stretched PP film 25 μm / LDPE film 30 μm laminated film 3
In No. 4, the microporous LDPE film 33 was sandwiched between the L-LDPE film 35 and the microporous LDPE film 33 so that the stretching direction was in the lateral direction of the bag, and the bag was made to be easily torn laterally. A non-sealing portion 22 was provided in a part of the vertical sealing portion 14 of the filter bag, and a U-shaped notch 23 was further provided in the non-sealing portion. The portion of the filter bag surrounded by the L-LDPE film 35 and the microporous LDPE film 33 is a stock solution chamber, and the laminated film 34 and the microporous LDPE film 33 are formed.
The part surrounded by is the test chamber.

When the above-mentioned filtration bag is used, the specimen and sterilized physiological saline are put in a stock solution chamber and heat-sealed, and a machine such as a stomacher is used to disperse the microorganisms in the specimen sufficiently in physiological saline. The suspension is transferred to a test solution chamber to prepare a test solution for measurement. The filter bag is removed from the machine and brought to a clean bench or the like, and the surface of the bag is wiped with a gauze or the like soaked with a disinfectant solution or a sterilizing solution to remove bacteria. Next, when the film is pulled laterally using the U-shaped notch 23, the laminated film 34 is torn as shown in FIG. 4, and the test solution outlet 26 is formed. Microorganisms can be measured by collecting the test liquid from the test liquid outlet 26 with a pipette or the like. Further, it can be transferred from the test liquid outlet 26 to another container.

[0017]

EFFECTS OF THE INVENTION The filtration bag constructed according to the present invention has a large area for the filtration film, so that the filtration rate is fast and the concentration of the microorganisms in the stock solution chamber and the test solution chamber, which are separated by the filtration film, easily reach equilibrium. The efficiency of sample preparation can be improved. Also,
Since the test solution chamber can be easily opened in a state where the stock solution chamber is sealed, the workability is good, the contamination of microorganisms from the outside is reduced, and the accuracy of microorganism measurement can be improved.

[Brief description of drawings]

FIG. 1 is a perspective view of a filter bag according to the present invention.

FIG. 2 is a sectional view of an opening of a filter bag according to the present invention.

FIG. 3 is a view in which a tear initiation portion is provided on a filter bag using a film having good tearability.

FIG. 4 is a view showing a state in which a tearable film is used and the tear opening portion is opened.

FIG. 5 is a cross-sectional view of the opening of the filtration bag manufactured in the first embodiment.

FIG. 6 is a perspective view of a filtration bag manufactured in the second embodiment.

[Explanation of symbols]

 1 Filtration Bag 11 Surface Film of Filtration Bag 12 Film for Filtration 13 Back Film of Filtration Bag 14 Vertical Sealing Part 15 Horizontal Sealing Part 16 Stock Solution Chamber 17 Test Solution Chamber 21 Surface Film Laminated with Stretched Film 22 Unsealing Part for Opening 23 Unsealing U-shaped notch for use 24 Seal portion of opening 25 Tear piece 31 ON film 32 LDPE film 33 Microporous LDPE film 34 Uniaxially stretched PP / LDPE laminated film 35 L-LDPE film

Claims (2)

[Claims] 1. A bag for preparing a sample for microbiological examination, which comprises a plastic film, characterized in that a microporous filtering film is sandwiched between plastic films and sealed on three sides to form a bag. bag. 2. A film having good tearability is used on at least one surface constituting a bag, and a non-sealing part is provided on a part of a sealing part in the tearing direction of the film on the film. The filter bag according to claim 1, wherein a notch for opening the opening is provided in the opening.