JP7453815B2 - plant activator - Google Patents

Description

The present invention relates to a plant activator.

Techniques for regulating plant growth have been developed with the aim of improving the supply efficiency of grain plants and garden plants. In addition to measures such as optimizing temperature and sunlight conditions and fertilization, methods have been reported for activating plants using plant activators that have plant growth regulating effects such as promoting growth, suppressing dormancy, and suppressing stress.

Patent Document 1 reports a plant activator characterized by containing an oxo fatty acid derivative or a salt or ester thereof as an active ingredient. The plant activator described in Patent Document 1 has an excellent resistance-inducing effect and growth-promoting effect on plants, but this activation for inducing plant resistance and/or promoting plant growth is difficult. The use of such agents has had a problem in that the effects of the activators may not last long after being applied to plants.

From the perspective of saving labor in agricultural work, etc., the sustainability of the effects of the activating components of plant activators is an issue. It is presumed that the insufficient sustainability of the effect of the plant activator described in Patent Document 1 is due to the possibility that the activator is washed away from the surface of the plant to which it is applied, and therefore does not remain in the plant body at a sufficient concentration. Ru. However, there is no effective means for making the activator remain, and there has been a strong desire for a technique for achieving satisfactory performance in terms of the sustainability of the effect of the activating component.

International Publication No. 2018/168860

Therefore, the present invention aims to provide a plant activator that improves the sustainability of the effect, has a long residual effect in the applied plant, and has an excellent disease resistance inducing effect and growth promoting effect. purpose.

The present invention provides at least one compound selected from oxo fatty acids, derivatives thereof, or salts thereof, hydroxylated fatty acids, derivatives thereof, or salts thereof, and unsaturated fatty acids (excluding oxo fatty acids and hydroxylated fatty acids). Contains a plant activator.

Preferably, the unsaturated fatty acid is an unsaturated fatty acid having 10 to 20 carbon atoms.

A plant activator in which the unsaturated fatty acid is selected from the monounsaturated or polyunsaturated fatty acid group consisting of oleic acid, linoleic acid, α-linolenic acid, γ-linolenic acid, arachidonic acid, etc. is preferred.

A plant activator in which the unsaturated fatty acid is linoleic acid is preferable.

The oxo fatty acid or its derivative or its salt has the following formula:
HOOC-(R ¹ )-CH=CH-C(=O)-R ² (I)
(In the formula,
R ¹ : a straight chain or branched, saturated or unsaturated hydrocarbon group containing 6 to 12 carbon atoms,
^R2 : an alkyl group having 2 to 8 carbon atoms, which may contain one or more branches and/or double bonds)
A plant activator that is an oxo fatty acid, a derivative thereof, or a salt thereof having the structural formula is preferred.

Preferably, the oxo fatty acid is an oxo fatty acid in which the R ¹ hydrocarbon group of the oxo fatty acid has 8 to 10 carbon atoms, and the R ² alkyl group has 4 to 6 carbon atoms.

A plant activator in which the oxo fatty acid contains a double bond in which R ¹ of the oxo fatty acid forms a conjugated double bond with the double bond between the α and β carbons of the carbonyl group in formula (I). is preferred.

The oxo fatty acid is preferably a plant activator in which R ¹ of the oxo fatty acid is a linear or branched hydrocarbon group having 9 carbon atoms, and R ² is an alkyl group having 5 carbon atoms.

A plant activator in which the oxo fatty acid is ketooctadecadienoic acid is preferred.

A plant activator in which the oxo fatty acid is 13-oxo-9,11-octadecadienoic acid is preferred.

The hydroxylated fatty acid or its derivative or its salt has the following formula (II) and/or (III):
HOOC-(R ³ )-CH(OH)-CH(OH)-CH=CH-CH(OH)-R ⁴ (II)
HOOC-(R ³ )-CH(OH)-CH=CH-CH(OH)-CH(OH)-R ⁴ (III)
(In the formula,
R ³ is a straight-chain or branched hydrocarbon group having from 4 to 12 carbon atoms, which may contain one or more double bonds and/or OH groups; , the position of the double bond is not limited,
R ⁴ is a straight-chain or branched hydrocarbon group having 2 to 8 carbon atoms, which may contain one or more double bonds and/or OH groups, including double bonds ), the position of the double bond is not limited)
A plant activator that is a hydroxylated fatty acid or a derivative or salt thereof having the structural formula is preferred.

The hydroxylated fatty acid is preferably a plant activator in which the R ³ hydrocarbon group has 6 to 8 carbon atoms and the R ⁴ hydrocarbon group has 4 to 6 carbon atoms.

The hydroxylated fatty acid has a structure in which R ³ is -(CH ₂ ) _n - (n is an integer from 4 to 12), and R ⁴ is C _n H _2n+1 - (n is 2 to 8). A plant activator having a structure of a certain integer) is preferable.

In the hydroxylated fatty acid, R ³ is an alkylene group having 7 carbon atoms (-(CH ₂ ) ₇ --), and R ⁴ is an alkyl group having 5 carbon atoms (CH ₃ CH ₂ CH ₂ CH ₂ CH ₂ -- ) is preferred.

A plant activator in which the hydroxylated fatty acid is hydroxyoctadecaenoic acid is preferable.

A plant activator in which the hydroxylated fatty acid is 9,10,13-trihydroxy-11-octadecaenoic acid is preferred.

A plant activator in which the hydroxylated fatty acid is 9,12,13-trihydroxy-10-octadecaenoic acid is preferred.

In addition, "octadecaenoic acid" is a conventional notation (for example, JP-A-3-14539, etc.), and the above-mentioned "9,10,13-trihydroxy-11-octadecaenoic acid" is "9,10, It is also written as ``13-trihydroxyoctadec-11-enoic acid'' or ``9,10,13-trihydroxy-11-octadecenoic acid.'' Similarly, the above-mentioned "9,12,13-trihydroxy-10-octadecaenoic acid" is replaced by "9,12,13-trihydroxyoctadec-10-enoic acid" or "9,12,13-trihydroxy- It is also written as "10-octadecenoic acid". In addition, in the examples, the manufacturer's description is also written in parentheses. Furthermore, the above explanation applies to all "octadecaenoic acid" used in the present specification, claims, drawings, and abstract.

The structural formula of "9,10,13-trihydroxy-11-octadecaenoic acid" is shown by the following structural formula (1).

The structural formula of "9,12,13-trihydroxy-10-octadecaenoic acid" is shown by the following structural formula (2).

The ratio of the at least one compound selected from the oxo fatty acid, a derivative thereof, or a salt thereof and the hydroxylated fatty acid, a derivative thereof, or a salt thereof to the unsaturated fatty acid is 10% to 90% by weight. A plant activator containing the unsaturated fatty acid is preferable.

Note that as the oxo fatty acid and hydroxylated fatty acid derivatives, esters of oxo fatty acid and hydroxylated fatty acid are desirable, respectively. Further, as the salts of the oxo fatty acids and hydroxylated fatty acids, salts such as sodium salts, potassium salts, and ammonium salts can be used, as will be described later.

It is preferable that the plant activator is a spray or dipping agent that is brought into contact with the leaves or roots of a plant, or a plant activator that is used as a soil irrigation agent.

The plant activator is preferably a plant activator used for plants selected from Brassicaceae, Poaceae, Fabaceae, Solanaceae, Rosaceae, Amaranthaceae, or Malvaceae.

The plant activator of the present invention has an excellent disease resistance-inducing effect and a growth-promoting effect, and since it is effectively prevented from being washed off from the plant body, these effects are highly sustainable.

1 is a graph showing the coexistence effect of unsaturated fatty acids on resistance to wash-off.

Plant Activator The plant activator of the present invention contains at least one compound selected from oxo fatty acids, derivatives thereof, or salts thereof, hydroxylated fatty acids, derivatives thereof, or salts thereof, and unsaturated fatty acids (however, oxo fatty acids and (excluding hydroxylated fatty acids).

In the present invention, "plant activation" means adjusting the growth activity of a plant in some way to activate or maintain it, and includes growth promotion (including expansion of stems and leaves, promotion of growth of tubers, etc.). This concept encompasses plant growth regulating effects such as suppressing dormancy, inducing and imparting plant resistance to stress (eg, diseases, etc.), and anti-aging.

The plant activator of the present invention contains at least one compound selected from oxo fatty acids, derivatives thereof, or salts thereof, and hydroxylated fatty acids, derivatives thereof, or salts thereof, as an active ingredient for exhibiting such plant activating effects. include. By containing at least one compound selected from such compounds, derivatives of such compounds, or salts thereof, the plant activator of the present invention can be applied to part of the foliage or roots of plants, thereby It can impart a growth promoting effect to plants. In plants inoculated with the plant activator of the present invention, increases in leaf length and leaf weight, which are indicators of plant growth, and promotion of tuber or tuberous root growth are confirmed compared to untreated plants. Therefore, it is considered that the plant activator of the present invention imparts a growth promoting effect to plants. By using the plant activator of the present invention, the growth of plants can be promoted and the yield of plants such as vegetables, grains, and fruits can be increased. The plant activator of the present invention has a very high plant growth promoting effect, and as a result, it can bring about an excellent yield increasing effect and improved harvest efficiency of commercial crops. Furthermore, the plant activator of the present invention can activate the salicylic acid pathway involved in inducing resistance in the plant by applying it to part of the stems, leaves, or roots of the plant, and as a result, the plant can be affected by diseases and other diseases. can induce resistance to

However, as mentioned above, although plant activators containing oxo fatty acid derivatives or their salts or esters as active ingredients have an excellent activating effect, they are not effective before or during the time when the active ingredients can produce a sufficient effect. For example, rain may cause the active ingredient to be washed away from the active site. In many applications involving topical inoculation of plant stimulants, such wash-off of the active ingredients in the plant stimulant by rain, wind erosion and other erosive forces can significantly reduce the potency and duration of action of the plant stimulant. may be reduced.

In addition to the above-mentioned active ingredients, that is, at least one compound selected from oxo fatty acids, derivatives thereof, or salts thereof, and hydroxylated fatty acids, derivatives thereof, or salts thereof, the plant activator of the present invention further contains unsaturated fatty acids. include. Note that the unsaturated fatty acids of the present invention do not include oxo fatty acids and hydroxylated fatty acids. The unsaturated fatty acids contained in the plant activator of the present invention are hydrophobic and have low water solubility. As a result, the unsaturated fatty acid of the present invention can impart water-repellent properties to the plant activator at the inoculation site. That is, the plant activator of the present invention is hardly washed off. Furthermore, the active ingredient oxo fatty acid or its derivative or its salt and/or hydroxylated fatty acid or its derivative or its salt is present so as to be included in the unsaturated fatty acid coexisting in the plant activator. Therefore, the plant activator of the present invention exhibits remarkable resistance to washing off by rain, etc., and the active ingredient adheres more strongly to the surface of the plant body such as leaves, resulting in the activation effect of the plant activator. The duration of is increased.

The oxo fatty acid or its derivative or its salt of the present invention has the following formula:
HOOC-(R ¹ )-CH=CH-C(=O)-R ² (I)
(In the formula,
R ¹ : A straight chain or branched, saturated or unsaturated hydrocarbon group containing 6 to 12 carbon atoms; R ² : One or more alkyl groups having 2 to 8 carbon atoms. (may contain branches and/or double bonds)
An oxo fatty acid having the structural formula or a derivative thereof or a salt thereof can be preferably used.

In one embodiment of the present invention, the hydrocarbon group of R ¹ in the oxo fatty acid has 8 to 10 carbon atoms, and the alkyl group of R ² has 4 to 6 carbon atoms. In another embodiment, R ¹ in the oxo fatty acid contains a double bond that forms a conjugated double bond with the double bond between the α and β carbons of the carbonyl group in formula (I). Furthermore, in another embodiment, R ¹ in the oxo fatty acid is preferably a linear or branched hydrocarbon group having 9 carbon atoms, and R ² is preferably an alkyl group having 5 carbon atoms.

Specifically, the oxo fatty acid of the present invention includes ketooctadecadienoic acid. For example, ketooctadecadienoic acids include, but are not limited to, 9-oxo-10,12-octadecadienoic acid (9-oxoODA), 13-oxo-9,11-octadecadienoic acid (13-oxoODA), 5-oxo-6,8-octadecadienoic acid, 6-oxo-9,12-octadecadienoic acid, 8-oxo-9,12-octadecadienoic acid, 10-oxo-8 , 12-octadecadienoic acid, 11-oxo-9,12-octadecadienoic acid, 12-oxo-9,13-octadecadienoic acid and 14-oxo-9,12-octadecadienoic acid and their isomers Examples include the body.

Note that esters are preferable as the oxo fatty acid derivatives. The esters of oxo fatty acids of the present invention include, but are not limited to, methyl esters, ethyl esters, propyl esters, butyl esters, pentyl esters, isopentyl esters, octyl esters, and the like. Salts of oxo fatty acids include ammonium salts such as alkylammonium salts such as ammonium salts and tetramethylammonium salts, alkaline earth metal salts such as calcium salts and magnesium salts, and sodium salts, lithium salts, and potassium salts. Examples include metal salts such as alkali metal salts, cobalt salts, manganese salts, etc., but the salts are not particularly limited as long as they are one or more agriculturally acceptable salts such as salts contained in fertilizers and the like.

As the hydroxylated fatty acid or its derivative or its salt of the present invention, the following formula (II) and/or (III)
HOOC-(R ³ )-CH(OH)-CH(OH)-CH=CH-CH(OH)-R ⁴ (II)
HOOC-(R ³ )-CH(OH)-CH=CH-CH(OH)-CH(OH)-R ⁴ (III)
(In the formula,
R ³ is a straight-chain or branched hydrocarbon group having 4 to 12 carbon atoms, which may contain one or more double bonds and/or OH groups, including double bonds , the position of the double bond is not limited,
R ⁴ is a straight-chain or branched hydrocarbon group having from 2 to 8 carbon atoms, which may contain one or more double bonds and/or OH groups; ), the position of the double bond is not limited)
A hydroxylated fatty acid having the structural formula or a derivative thereof or a salt thereof may be preferably used.

In one embodiment of the invention, the R ³ hydrocarbon group in the hydroxylated fatty acid has 6 to 8 carbon atoms, and the R ⁴ hydrocarbon group has 4 to 6 carbon atoms. In another embodiment, R ³ in the hydroxylated fatty acid has a structure of -(CH ₂ ) _n - (n is an integer from 4 to 12), and R ⁴ has a structure of C _n H _2n+1 - (n is an integer from 2 to 8). Furthermore, in another embodiment, R ³ in the hydroxylated fatty acid is an alkylene group having 7 carbon atoms (-(CH ₂ ) ₇ --), and R ⁴ is an alkyl group having 5 carbon atoms (CH ₃ CH ₂ CH ₂ CH ₂ CH ₂ -) is preferred.

A specific example of the hydroxylated fatty acid of the present invention is hydroxyoctadecaenoic acid. For example, hydroxyoctadecaenoic acid includes, but is not limited to, 9,10,13-trihydroxy-11-octadecaenoic acid and/or 9,12,13-trihydroxy-10-octadecaenoic acid and These include isomers.

Note that esters are preferable as the hydroxylated fatty acid derivatives. Esters of hydroxylated fatty acids of the present invention include, but are not limited to, methyl esters, ethyl esters, propyl esters, butyl esters, pentyl esters, isopentyl esters, octyl esters, and the like. Salts of hydroxylated fatty acids include ammonium salts such as alkylammonium salts such as ammonium salts and tetramethylammonium salts, alkaline earth metal salts such as calcium salts and magnesium salts, sodium salts, lithium salts, potassium salts, etc. Examples include alkali metal salts such as salts, metal salts such as cobalt salts, manganese salts, etc., but there is no particular limitation as long as it is one or more types of agriculturally acceptable salts such as salts contained in fertilizers.

The unsaturated fatty acids of the present invention are not particularly limited, but unsaturated fatty acids having about 10 to 20 carbon atoms may be preferable from the viewpoint of low solubility in water. For example, unsaturated fatty acids having 18 carbon atoms are more preferred. Moreover, in the unsaturated fatty acid of the present invention, the number of unsaturated bonds in its molecular structure is not limited, and it may be a monounsaturated fatty acid or a polyunsaturated fatty acid. For example, unsaturated fatty acids include, but are not limited to, oleic acid, linoleic acid, oleic acid, α-linolenic acid, γ-linolenic acid, arachidonic acid, and the like. In addition, the unsaturated fatty acids may be in either the form of free fatty acids or their salts (commonly usable salts, such as the salts listed above), and one or more of these may be used. can be selected. As the unsaturated fatty acid of the present invention, commercially available products may be used, or synthetic products from commercially available compounds may be used. Moreover, one type of unsaturated fatty acid may be used as the unsaturated fatty acid, or two or more types may be used.

In addition, when isomers exist in the compounds exemplified in this specification, all possible isomers can be used in the present invention unless otherwise specified.

The plant activator of the present invention comprises at least one compound selected from at least one oxo fatty acid, a derivative thereof, or a salt thereof, and at least one hydroxylated fatty acid, a derivative thereof, or a salt thereof, and at least one unsaturated fatty acid. including. That is, the unsaturated fatty acids that can be used in the present invention are not limited to compatibility with any particular type of oxo fatty acids or derivatives or salts thereof or hydroxylated fatty acids or derivatives thereof or salts thereof, but can be used in a wide range of ways. A wide range of unsaturated fatty acids can be used in the plant stimulant of the present invention together with oxo fatty acids or derivatives or salts thereof and hydroxylated fatty acids or derivatives or salts thereof. For example, the plant activator of the present invention includes at least one oxo fatty acid selected from 13-oxoODA and 9-oxoODA, a derivative thereof, or a salt thereof, 9,10,13-trihydroxy-11-octadecaenoic acid and/or 9, at least one compound selected from at least one hydroxylated fatty acid selected from 12,13-trihydroxy-10-octadecaenoic acid, a derivative thereof, or a salt thereof, and oleic acid, linoleic acid, α-linolenic acid, γ - May contain at least one unsaturated fatty acid selected from linolenic acid and arachidonic acid. For example, the plant activator of the present invention comprises at least one compound selected from ketooctadecadienoic acid or a derivative thereof or a salt thereof, hydroxyoctadecaenoic acid or a derivative thereof or a salt thereof, and oleic acid or linoleic acid. , α-linolenic acid, γ-linolenic acid, and arachidonic acid. For example, the plant activator of the present invention may contain 13-oxo-9,11-octadecadienoic acid or a derivative thereof or a salt thereof and linoleic acid, and 9,10,13-trihydroxy-11- It may contain octadecaenoic acid and/or 9,12,13-trihydroxy-10-octadecaenoic acid or its derivative or its salt and linoleic acid, and 13-oxo-9,11-octadecadienoic acid and/or It may contain 9,10,13-trihydroxy-11-octadecaenoic acid and/or 9,12,13-trihydroxy-10-octadecaenoic acid or a derivative or salt thereof and linoleic acid.

Since the plant activator of the present invention contains an unsaturated fatty acid, washing of the plant activator from the plant after inoculation can be minimized. Moreover, in the plant activator of the present invention, the coexistence of unsaturated fatty acids allows for the activation of at least one active ingredient selected from oxo fatty acids, derivatives thereof, or salts thereof, and hydroxylated fatty acids, derivatives thereof, or salts thereof. The excellent disease resistance-inducing effect and growth-promoting effect of the compound does not decrease or disappear. Moreover, unsaturated fatty acids do not have a negative effect on the plants to which they are applied. As a result, enhanced efficacy as a plant stimulant and its longevity can be achieved. Therefore, the plant activator of the present invention can reduce the frequency of application of the plant activator to plants, and also reduce the amount of the plant activator applied.

As mentioned above, the plant activator of the present invention contains at least one compound selected from naturally occurring oxo fatty acids, derivatives thereof, or salts thereof, hydroxylated fatty acids, derivatives thereof, or salts thereof, and natural inorganic compounds. It is a plant activator containing saturated fatty acids. Therefore, the plant activator of the present invention is also very excellent in that it has less environmental impact.

In the plant activator of the present invention, the unsaturated fatty acid and at least one compound selected from the active ingredient for the activating effect, that is, oxo fatty acid or its derivative or its salt, and hydroxylated fatty acid or its derivative or its salt, are, for example The ratio of at least one compound selected from oxo fatty acids, derivatives thereof, or salts thereof and hydroxylated fatty acids, derivatives thereof, or salts thereof to unsaturated fatty acids is preferably about 90% or less by weight. . When the ratio of at least one compound selected from oxo fatty acids, derivatives thereof, or salts thereof and hydroxylated fatty acids, derivatives thereof, or salts thereof to unsaturated fatty acids is greater than 90%, the hydrophobic component in the plant activator is Since the content is low, there is a possibility that washing-off may not be effectively prevented. Further, the unsaturated fatty acid and at least one compound selected from the active ingredient for activation effect, ie, oxo fatty acid or its derivative or its salt, and hydroxylated fatty acid or its derivative or its salt, are, for example, The proportion of at least one compound selected from oxo fatty acids, derivatives thereof, or salts thereof and hydroxylated fatty acids, derivatives thereof, or salts thereof is preferably about 10% or more by weight. If the ratio of at least one compound selected from oxo fatty acids or derivatives thereof or salts thereof and hydroxylated fatty acids or derivatives thereof or salts thereof to unsaturated fatty acids is less than 10%, the activating effect in the plant activator Since the amount of active ingredients is small, the plant activation effect may be insufficient. For example, the ratio of at least one compound selected from oxo fatty acids, derivatives thereof, or salts thereof and hydroxylated fatty acids, derivatives thereof, or salts thereof to unsaturated fatty acids in the plant activator is about 10% or more by weight, It is about 90% or less. By containing the active ingredient for the activating effect and the unsaturated fatty acid in such a ratio in the plant activator, a good activating effect and resistance to washing off can be obtained.

The plant activator of the present invention may contain a diluent or carrier suitable for use as a plant activator, if necessary. These additive components are not particularly limited as long as they are agriculturally acceptable agents. In addition, components other than the diluent and carrier that are commonly used in agricultural chemical formulations may be further contained.

The stabilized plant activator of the present invention can be applied to plants by any method. For example, it can be used as a spray or immersion agent for contacting the leaves or roots of plants, or as a soil irrigation agent. The specific application method can be selected as appropriate depending on the cultivated plants to be applied and the form of use, but examples include ground liquid spraying, ground solid spraying, aerial liquid spraying, aerial solid spraying, liquid surface spraying, in-facility application, and soil mixing. Examples include application, soil irrigation application, surface treatment such as coating treatment, seedling raising box application, single flower treatment, and plant base treatment. In the plants to which it is applied, the plant activator of the present invention imparts a plant growth promoting effect and resistance to stresses such as diseases. Furthermore, the plant activator of the present invention has high resistance to washing off, and therefore, among the above application methods, it may be particularly preferable for application to treat the foliage of cultivated plants.

Plants to which the plant activator of the present invention can be applied are not particularly limited, and it can be successfully used for plants in general. For example, it can be suitably applied to plants of the family Brassicaceae, Poaceae, Fabaceae, Solanaceae, Rosaceae, Amaranthaceae, or Malvaceae. Moreover, the plants to be applied are not limited to wild-type plants, and may be, for example, mutants or transformants. Moreover, the variety of each plant is not particularly limited.

Although the present invention will be explained based on examples, the present invention is not limited to the examples only.

<Preparation of plant activator>
Example 1
13-oxo-9,11-octadecadienoic acid (13-oxo-9,11-octadecadienoic acid (13 -oxoODA) ((9Z,11E)-13-oxo-9,11-octadecadienoic acid, manufactured by Cayman Chemical Co., Ltd., 100 μg/100 μL ethanol solution) was used. In addition, linoleic acid (primary linoleic acid manufactured by Fujifilm Wako Pure Chemical Industries, Ltd.) was used as the unsaturated fatty acid. 5 μL (5 μg) of 13-oxoODA was added to 50 μg of linoleic acid and mixed. This was used as the test plant activator of Example 1 (ratio of oxo fatty acid to linoleic acid = 10%).
Example 2
As in Example 1, 13-oxoODA was used as the oxo fatty acid and linoleic acid was used as the unsaturated fatty acid. 45 μL (45 μg) of 13-oxoODA was added to 50 μg of linoleic acid and mixed. This was used as the test plant activator of Example 2 (ratio of oxo fatty acid to linoleic acid = 90%).
Example 3
9,10,13-trihydroxy-11-octadecaenoic acid, which is hydroxyoctadecaenoic acid, as at least one compound selected from oxo fatty acids, derivatives thereof, or salts thereof, and hydroxylated fatty acids, derivatives thereof, or salts thereof; (Manufactured by Lalaudan Fine Chemicals, 9(S), 10(S), 13(S)-trihydroxy-11(E) -octadecenoic acid (9(S), 10(S), 13 in English) (S)-trihydroxy-11(E)-octadecenoic acid), 200 mg/L ethanol solution), and 9,12,13-trihydroxy-10-octadecenoic acid (manufactured by Lalaudan Fine Chemicals, 9(S), 12 (S),13(S)-trihydroxy-10(E) -octadecenoic acid (9(S),12(S),13(S)-trihydroxy-10(E)-octadecenoic acid) ) and 200 mg/L ethanol solution) were mixed at a ratio of 2:1. First, 200 μL (40 μg) of 9(S), 10(S), 13(S)-trihydroxy-11(E) -octadecenoic acid (200 mg/L ethanol solution) and 9(S), 12( 100 μL (20 μg) of S),13(S)-trihydroxy-10(E) -octadecenoic acid (200 mg/L ethanol solution) was mixed to prepare 300 μL of a hydroxylated fatty acid mixture. In addition, linoleic acid (primary linoleic acid manufactured by Fujifilm Wako Pure Chemical Industries, Ltd.) was used as the unsaturated fatty acid. 25 μL of this hydroxylated fatty acid mixture (5 μg of hydroxylated fatty acid mixture) was added to 50 μg of linoleic acid and mixed. This was used as the test plant activator of Example 3 (ratio of hydroxylated fatty acid to linoleic acid = 10%).
Example 4
Similarly to Example 3, 9(S), 10(S), 13(S)-trihydroxy-11(E) -octadecenoic acid and 9(S), 12(S), 13 were used as hydroxylated fatty acids. A 2:1 hydroxylated fatty acid mixture of (S)-trihydroxy-10(E) -octadecenoic acid and linoleic acid were used as the unsaturated fatty acid. 225 μL of this hydroxylated fatty acid mixture (45 μg of hydroxylated fatty acid mixture) was added to 50 μg of linoleic acid and mixed. This was used as the test plant activator of Example 3 (ratio of hydroxylated fatty acid to linoleic acid = 90%).
Comparative example 1
13-oxoODA (100 μg/100 μL ethanol solution) of Example 1 was used as a test plant activator in Comparative Example 1 that does not contain linoleic acid.
Comparative example 2
The 200 μg/mL hydroxylated fatty acid mixture prepared in Example 3 was used as the test plant activator of Comparative Example 2, which does not contain linoleic acid.

<Evaluation method>
The test plant activators prepared in Examples 1 to 4 and Comparative Examples 1 and 2 above were tested and evaluated in the following manner.

<Water repellency test>
A water repellency test was conducted on the test plant activators of Examples 1 to 4 and Comparative Examples 1 and 2 that do not contain unsaturated fatty acids. The water used in the water repellency test was ion-exchanged water. In this water repellency test, first, a polypropylene resin plate was prepared, and spinach leaves with a length of about 15 cm were fixed to this resin plate. An evaluation surface for each test plant activator was formed by spreading and applying 5 μL of each test plant activator with a spatula onto spinach leaves in a width of 1 cm in the length direction and drying. The resin plate on which the spinach leaves were fixed was fixed to a jig so that the angle of inclination of the evaluation surface to the horizontal was 24 degrees, and 50 μL of ion-exchanged water was applied to the spinach leaves from a position 2.5 cm above each evaluation surface. A drop was applied along the length of the leaf to pass through the evaluation surface, and the time required for the drop to flow down an area of 11.5 cm, including the evaluation surface (1 cm), from the droplet position on the spinach leaf was measured. did. The results are shown in Figure 1.

As shown in FIG. 1, water flowed down quickly when the evaluation surface of the test plant activator (Examples 1 to 4) containing unsaturated fatty acids was included. That is, it was found that the surface coated with the test plant activator containing unsaturated fatty acids had high water repellency.

Therefore, a plant activator containing unsaturated fatty acids such that the ratio of oxo fatty acids, derivatives thereof, or salts thereof, or hydroxylated fatty acids, derivatives thereof, or salts thereof to unsaturated fatty acids is 10% to 90% by weight. When applied, the resistance of the plant activator to contact with fluid (water) is suppressed. As a result, the plant activator applied to the plant becomes resistant to being washed away by rain or the like that continues to fall after application. As a result, in the plant revitalizing agent of the present invention, the active ingredient is less likely to run off and adheres to the surface of the plant body, such as a leaf, for a longer period of time, so that the duration of the plant revitalizing effect can be increased.

From the above results, it can be seen that the plant activator of the present invention is a plant activator that is resistant to washing off, has excellent sustainability, and has a high plant activating effect.

Claims (4)

A plant activator containing at least one compound selected from oxo fatty acids, derivatives thereof, or salts thereof and hydroxylated fatty acids, derivatives thereof, or salts thereof, and unsaturated fatty acids (excluding oxo fatty acids and hydroxylated fatty acids) The oxo fatty acid is 13-oxo-9,11-octadecadienoic acid, and the hydroxylated fatty acid is 9,10,13-trihydroxy-11-octadecenoic acid or 9,12, A plant activator that is 13-trihydroxy-10-octadecenoic acid . The ratio of the at least one compound selected from the oxo fatty acid, a derivative thereof, or a salt thereof and the hydroxylated fatty acid, a derivative thereof, or a salt thereof to the unsaturated fatty acid is 10% to 90% by weight. The plant activator according to claim 1 , which contains the unsaturated fatty acid so as to be. The plant activator according to claim 1 or 2 , which is used as a spray or immersion agent that is brought into contact with the leaves or roots of plants, or as a soil irrigation agent. According to any one of claims 1 to 3 , the plant activator is used for plants selected from Brassicaceae, Poaceae, Fabaceae, Solanaceae, Rosaceae, Amaranthaceae, or Malvaceae. The plant activator described.

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