JP6869455B2 - Manufacturing method of cell filling container, cell filling container, and sealing device - Google Patents

Description

The present invention relates to a manufacturing method for obtaining a cell-filled container by filling a container with a liquid containing cells, a cell-filling container, and a device for sealing the cell-filled container.

In the field of regenerative medicine, cells collected from a living body such as umbilical cord blood and stem cells may be used. After being collected from a living body, these cells are frozen and stored as a cell suspension. Various containers for cryopreserving cell suspensions have been devised (Patent Documents 1 to 3).

For example, Patent Document 1 discloses a cell cryopreservation container in which a plurality of storage chambers are provided at intervals, and adjacent storage chambers are communicated with each other by a communication portion. According to this cell cryopreservation container, the cell suspension of stem cells is cryopreserved in a plurality of storage chambers, so that each storage chamber can be separated and then thawed.

Japanese Unexamined Patent Publication No. 2013-5825 JP-A-2009-82732 Special Table 2001-517103 Gazette

When the cell suspension is frozen and stored, it is required that the same cell suspension used for regenerative medicine is stored as a test sample for quality control or the like. For example, if the cell suspension is contaminated due to a storage container, the cell suspension in contact with the contaminated containment chamber needs to be stored as a test sample.

The plurality of containment chambers as described above are communicated by a communication passage when the cell suspension is injected, and after the cell suspension is injected, the communication passage is closed and each storage chamber is a closed space. Be made. Closure of the communication passage is performed by heat welding, for example, if the container is made of a thermoplastic synthetic resin. If the cell suspension is heated during this heat welding, it may damage the cells.

The present invention has been made in view of the above-mentioned circumstances, and an object of the present invention is to provide a method for producing a cell-filled container and a cell-filled container suitable for storing a test sample of a liquid containing cells. ..

Another object of the present invention is to provide a sealing means in which damage to cells is suppressed in a cell-filled container for storing a liquid containing cells.

(1) The present invention is connected to a first accommodating portion for partitioning a first accommodating chamber having at least one port via the first accommodating portion and the first connecting portion, and has at least one port. A container provided with a second accommodating portion for partitioning a second accommodating chamber and at least two communication passages provided in the first connecting portion and communicating the first accommodating chamber and the second accommodating chamber. The present invention relates to a method for producing a cell-filled container, which is filled with a liquid containing cells to obtain a cell-filled container. The method for producing the cell filling container is to fill the first storage chamber with the liquid in a state where the liquid does not flow into the second storage chamber through the communication passage until the first storage chamber is filled with the liquid. The first step is to allow the liquid filled in the first storage chamber to flow into the second storage chamber through the communication passage.

Since the liquid filled in the first storage chamber in the first step flows into the second storage chamber in the second step, the liquid suitable for the test sample is stored in the second storage chamber.

(2) In the first step, the second storage chamber is positioned above the first storage chamber in the direction of gravity, the first storage chamber is filled with the liquid, and in the second step, the first 2 The storage chamber may be positioned below the first storage chamber in the direction of gravity, and the liquid filled in the first storage chamber may flow into the second storage chamber through the communication passage.

Since the liquid can be filled in the first storage chamber and the liquid can be flowed from the first storage chamber to the second storage chamber by using gravity, each step can be easily realized.

(3) In the first step, at least the first storage chamber may be depressurized, and then the first storage chamber may be filled with the liquid.

As a result, the liquid can be filled without communicating the first storage chamber with the atmosphere.

(4) In the second step, after filling the second storage chamber with the liquid, the gas remaining in the first storage chamber may be removed.

As a result, the influence of the atmosphere in the first containment chamber can be suppressed.

(5) In the second step, after filling the second storage chamber with the liquid, the communication passage of the first connecting portion may be sealed.

Thereby, the first accommodation chamber and the second accommodation chamber can be separated.

(6) The volume of the second storage chamber may be smaller than the volume of the first storage chamber.

This makes it easier for the second containment chamber to be filled with the liquid filled in the first containment chamber.

(7) In the second step, the communication passage of the first connecting portion may be pressed to close the passage, and the pressed portion may be heat-welded to seal.

Since the portion where the communication passage is pressed and blocked is heat-welded, there is no liquid containing cells in the location where the heat-welding is performed. Therefore, it is difficult to transfer the heat during heat welding to the liquid containing cells.

(8) Using a sealing member in which a heat generating member is arranged between the first heat insulating material and the second heat insulating material, on the first accommodating portion side and the second accommodating portion side of the communication passage of the first connecting portion. The first heat insulating material and the second heat insulating material may be arranged and pressed, and heat welding may be performed between the first heat insulating material and the second heat insulating material by the heat generating member.

Since the heat-generating member heats and heat-welds while the communication passage is pressed and blocked by the first heat insulating material and the second heat insulating material, the liquid containing cells does not exist at the heat-welded part, and heat is generated. It is difficult to transfer the heat during welding to the liquid containing cells.

(9) The container is connected to the opposite side of the first accommodating portion via the second accommodating portion and the second accommodating portion, and is a third accommodating chamber for partitioning a third accommodating chamber having at least one port. Further, at least two communication passages provided in the second connecting portion and communicating the second accommodating chamber and the third accommodating chamber are further provided, and in the second step, the portion is further provided. The liquid filled in the first storage chamber may flow into the third storage chamber.

This gives a plurality of test samples.

(10) The sum of the volume of the second accommodation chamber and the volume of the third accommodation chamber may be smaller than the volume of the first accommodation chamber.

This makes it easier for the second and third containment chambers to be filled with the liquid filled in the first containment chamber.

(11) The cell filling container according to the present invention is connected to a first storage portion that partitions a first storage chamber having at least one port, via the first storage portion and the first connecting portion. A second accommodating portion for partitioning a second accommodating chamber having at least one port is connected to the opposite side of the first accommodating portion via the second accommodating portion and the second accommodating portion, and at least one port. A third accommodating portion for partitioning the third accommodating chamber, at least two communication passages provided in the first connecting portion and communicating the first accommodating chamber and the second accommodating chamber, and the first accommodating chamber. It is provided in the two connecting portions, and includes at least two communication passages that communicate the second accommodation chamber and the third accommodation chamber. The sum of the volume of the second accommodation chamber and the volume of the third accommodation chamber is smaller than the volume of the first accommodation chamber.

As a result, the liquid filled in the first storage chamber can be surely flowed into the second storage chamber and the third storage chamber to be filled.

(12) Each connecting passage provided in the first connecting portion and each connecting passage provided in the second connecting portion are along the first direction in which the first accommodating portion and the second accommodating portion are lined up. The first accommodating portion, the second accommodating portion, and the third accommodating portion are the first accommodating portion, the second accommodating portion, and the third accommodating portion. The length of the outer shape in the third direction orthogonal to the second direction is shorter than the length of the outer shape in the second direction, and each communication passage provided in the first connecting portion and the second connecting portion are provided. Each of the communication passages provided in the above-mentioned first accommodating portion, the second accommodating portion, and the third accommodating portion is arranged so as to be biased to one of the second directions from the center position of the outer shape of the third accommodating portion in the second direction. You may.

As a result, it is easy to seal each of the communication passages provided in the first connecting portion and the second connecting portion by heat welding or the like.

(13) Each communication passage provided in the first connecting portion and each opening in which each communication passage provided in the second connecting portion communicates with the second storage chamber or the third storage chamber is the cell. The filling container is located at the uppermost position in the gravity direction in the second storage chamber or the third storage chamber in a state where the first storage portion is on the upper side in the gravity direction and the third storage portion is on the lower side in the gravity direction. It may be something to do.

As a result, when the first accommodating portion is on the upper side and the third accommodating portion is on the lower side, air can easily escape from the second accommodating chamber and the third accommodating chamber.

(14) The present invention includes a first accommodating portion that partitions the first accommodating chamber, and a second accommodating portion that is connected to the first accommodating portion via the first connecting portion and partitions the second accommodating chamber. , The container provided in the first connecting portion and having a communication passage connecting the first storage chamber and the second storage chamber is filled with a liquid containing cells and the communication passage is sealed. The present invention relates to a method for producing a cell-filled container to obtain a cell-filled container. The method for producing a cell-filled container includes a first step of pressing and closing the communication passage, and a second step of heat welding the pressed portion of the communication passage.

Since the portion where the communication passage is pressed and blocked is heat-welded, there is no liquid containing cells in the location where the heat-welding is performed. Therefore, it is difficult to transfer the heat during heat welding to the liquid containing cells.

(15) In the second step, the heat-welded portion may be cut to separate the first accommodating portion and the second accommodating portion.

As a result, the first accommodating portion and the second accommodating portion can be stored in different places.

(16) In the first step, the communication passage may be pressed by a heat insulating material.

This makes it more difficult for the heat during heat welding to be transferred to the liquid containing the cells.

(17) The present invention is connected to a first storage portion that partitions a first storage chamber filled with a liquid containing cells via the first storage portion and the first connecting portion, and is a liquid containing cells. A second accommodating portion for partitioning a second accommodating chamber filled with cells and a first connecting portion are provided, and a liquid containing cells can be communicated between the first accommodating chamber and the second accommodating chamber so that a liquid containing cells can flow. The present invention relates to a sealing device for sealing a cell-filled container provided with a communication passage. The sealing device includes a first heat insulating material and a second heat insulating material that press and close the first accommodating portion side and the second accommodating portion side of the communication passage, respectively, and the first heat insulating material and the second heat insulating material. It is provided with a heat generating member which is arranged between the materials and presses the connecting passage to heat-weld.

Since the heat-generating member heats and heat-welds while the communication passage is pressed and blocked by the first heat insulating material and the second heat insulating material, the liquid containing cells does not exist at the heat-welded part, and heat is generated. It is difficult to transfer the heat during welding to the liquid containing cells.

According to the present invention, a liquid test sample containing cells filled in the first containment chamber can be stored in the second containment chamber.

According to the present invention, damage to cells is suppressed when sealing a cell-filled container filled with a liquid containing cells.

FIG. 1 is a perspective view showing a container 10 according to an embodiment of the present invention. FIG. 2 is a cross-sectional view showing the internal structure of the container 10. FIG. 3 is a cross-sectional view showing a state of the container 10 in the first step. FIG. 4 is a cross-sectional view showing a state in which the cell suspension 40 is filled in the first storage chamber 14 in the first step. FIG. 5 is a cross-sectional view showing a state of the container 10 in the second step. FIG. 6 is a cross-sectional view showing a state in which the cell suspension 40 has flowed from the first storage chamber 14 into the second storage chamber 22 and the third storage chamber 27 in the second step. FIG. 7 is a cross-sectional view showing a state in which the passages 23, 24, 29, and 30 are sealed in the second step. FIG. 8 is a cross-sectional view showing a state in which the first accommodating portion 11, the second accommodating portion 12, and the third accommodating portion 13 are separated in the second step. FIG. 9 is a schematic view showing a main configuration of the sealing device 60. FIG. 10 is a diagram showing the configuration of the first heater unit 63. FIG. 11 is a diagram showing a state in which a part 43 of the first connecting portion 21 of the container 10 is pressed by the heat insulating materials 71 and 72. FIG. 12 is a diagram showing a state in which the sealing portion 42 is formed on the first connecting portion 21 of the container 10.

Hereinafter, embodiments of the present invention will be described with reference to the drawings. It goes without saying that the present embodiment is merely an example of the present invention, and the present embodiment may be appropriately modified as long as the gist of the present invention is not changed.

[Container 10]
As shown in FIGS. 1 and 2, the container 10 (corresponding to the cell filling container) is for cryopreserving the cell suspension 40 (see FIG. 4). The container 10 is formed by, for example, sticking two resin sheets molded into a shape described later.

The container 10 has a first accommodating portion 11, a second accommodating portion 12, and a third accommodating portion 13. The first accommodating portion 11 has a first accommodating chamber 14 which is an internal space, and has a length of 52 in the left-right direction (corresponding to the first direction) and 53 in the vertical direction (corresponding to the second direction). It is a flat shape with the shortest length in the front-rear direction 51 (corresponding to the third direction). The first accommodation chamber 14 is a space having a substantially rectangular shape, and the upper portion in the vertical direction 53 has the maximum length in the horizontal direction 52, and the lower portion in the vertical direction 53 has the length in the horizontal direction 52. It is curved in a funnel shape that gradually shortens downward. The volume of the first storage chamber 14 is appropriately set in consideration of the amount of the cell suspension 40 to be stored, and is, for example, about 20 mL.

The first storage unit 11 is provided with a cell injection port 15, a spare port 16, and a cell recovery port 17 leading to the first storage chamber 14. The cell injection port 15 is the upper end of the first storage chamber 14 in the vertical direction 53, is provided near the left end in the left-right direction 52 (the left end of the first storage portion 11 in FIG. 1), and is open to the outside. The first containment chamber 14 and the outside are communicated with each other. A tube 18 is connected to the cell injection port 15 via a cylindrical member 33. The cylindrical member 33 is a cylindrical member made of rubber, an elastomer, or the like, and is liquid-tightly inserted into the cell injection port 15. The cylindrical member 33 does not easily block the cell injection port 15 even if it receives an external force or the like, or the first storage chamber 14 is depressurized. The tube 18 is a resin tube. One end of the tube 18 is liquidtightly connected to the cylindrical member 33. The cell suspension 40 is circulated through the tube 18 and the cell suspension 40 is injected into the first containment chamber 14 through the cell injection port 15.

The spare port 16 is the upper end of the first accommodation chamber 14 in the vertical direction 53, and is provided near the right end in the left-right direction 52 (the right end of the first accommodation portion 11 in FIG. 1). The upper end of the spare port 16 is sealed and does not open to the outside. When the spare port 16 is used, the upper end side of the spare port 16 is cut and opened. A cylindrical stopper 34 is liquid-tightly inserted into the spare port 16. In the stopper 34, a cylindrical cylinder and a film that divides the internal space of the cylinder into one end side (upper side in FIG. 2) and the other end side (lower side in FIG. 2) are integrally formed of rubber, elastomer, or the like. It is formed. The plug 34 does not easily block the spare port 16 even if it receives an external force or the pressure in the first storage chamber 14 is reduced. Further, even if the upper end side of the spare port 16 is opened by the stopper 34, the liquid does not immediately flow out from the first storage chamber 14. When accessing the first storage chamber 14 from the outside, an injection needle or the like that can penetrate the membrane of the plug 34 is punctured. The membrane of the plug 34 stabilizes the posture of the punctured injection needle or the like. The spare port 16 is used, for example, when injecting a drug or the like into the cell suspension 40 stored in the first storage chamber 14.

The cell recovery port 17 is provided at the lower end of the first storage chamber 14 in the vertical direction 53. The lower end of the cell recovery port 17 is sealed and does not open to the outside. When the cell recovery port 17 is used, the lower end side of the cell recovery port 17 is cut and opened. A cylindrical plug 35 is liquid-tightly inserted into the cell recovery port 17. In the stopper 35, a cylindrical cylinder and a film that divides the internal space of the cylinder into one end side (upper side in FIG. 2) and the other end side (lower side in FIG. 2) are integrated by rubber, elastomer, or the like. It was formed in. The plug 35 does not easily block the cell recovery port 17 even if it receives an external force or the pressure in the first storage chamber 14 is reduced. Further, even if the lower end side of the cell recovery port 17 is opened by the plug 35, the liquid does not immediately flow out from the first storage chamber 14. When accessing the first storage chamber 14 from the outside, an injection needle or the like that can penetrate the membrane of the stopper 35 is punctured. The membrane of the stopper 35 stabilizes the posture of the punctured injection needle or the like. The cell recovery port 17 is used when the cell suspension 40 stored in the first storage chamber 14 is discharged.

A hanging hole 19 is provided at the upper end of the first accommodating portion 11 between the cell injection port 15 and the spare port 16. The hanging hole 19 is arranged near the center of the first accommodating portion 11 in the left-right direction 52. The hanging hole 19 is used when the first accommodating portion 11 in a state where the second accommodating portion 12 and the third accommodating portion 13 are cut and separated is suspended and stored.

The second accommodating portion 12 is connected to the first accommodating portion 11 via the first connecting portion 21. The second accommodating portion 12 is arranged on the right side (right side in FIG. 1) of the first accommodating portion 11 in the left-right direction 52. The second accommodating portion 12 has a second accommodating chamber 22 which is an internal space, and has a flat shape in which the length in the front-rear direction 51 is the shortest as compared with the lengths in the left-right direction 52 and the up-down direction 53. .. The second accommodation chamber 22 is a space having a substantially rectangular shape, and the upper portion in the vertical direction 53 has the maximum length in the horizontal direction 52, and the lower portion in the vertical direction 53 has the length in the horizontal direction 52. It is curved in a funnel shape that gradually shortens downward. The volume of the second storage chamber 22 is smaller than the volume of the first storage chamber 14, for example, about 5 mL.

The first connecting portion 21 connects the first accommodating portion 11 and the second accommodating portion 12 at the upper portion in the vertical direction 53. The first connecting portion 21 is formed by, for example, two resin sheets constituting the container 10 being brought into close contact with each other. The length of the outer shape of the first connecting portion 21 in the front-rear direction 51 is shorter than the length in the vertical direction 53. That is, the first connecting portion 21 has a flat shape in the front-rear direction 51.

The first connecting portion 21 is provided with communication passages 23 and 24 extending in the left-right direction 52. The communication passages 23 and 24 are arranged so as to be separated from each other in the vertical direction 53, and each communicates the first accommodation chamber 14 and the second accommodation chamber 22. The communication passages 23 and 24 are arranged at positions biased upward from the center of the outer shape of the first accommodating portion 11 and the second accommodating portion 12 in the vertical direction 53. Further, the communication passages 23 and 24 are opened at the ends of the first accommodation chamber 14 and the second accommodation chamber 22 in the left-right direction 52 at the position where the length of the left-right direction 52 is maximized (upper portion).

The second storage chamber 22 is provided with a sample collection port 25. The sample collection port 25 is provided at the lower end of the second storage chamber 22 in the vertical direction 53. The lower end of the sample collection port 25 is sealed and does not open to the outside. When the sample collection port 25 is used, the lower end side of the sample collection port 25 is cut and opened. A cylindrical stopper 36 is liquid-tightly inserted into the sample collection port 25. Since the structure of the stopper 36 is the same as that of the stopper 35, detailed description thereof will be omitted here. The sample collection port 25 is used when the cell suspension 40 stored in the second storage chamber 22 is discharged.

The third accommodating portion 13 is connected to the second accommodating portion 12 via the second connecting portion 26. The third accommodating portion 13 is arranged on the right side (right side in FIG. 1) of the second accommodating portion 12 in the left-right direction 52. That is, the third accommodating portion 13 is arranged on the side opposite to the first accommodating portion 11 with respect to the second accommodating portion 12. The third accommodating portion 13 has a third accommodating chamber 27 which is an internal space, and has a flat shape having the shortest length in the front-rear direction 51 as compared with the lengths in the left-right direction 52 and the up-down direction 53. .. The third storage chamber 27 is a space having a substantially rectangular parallelepiped shape. The volume of the third storage chamber 27 is smaller than the volumes of the first storage chamber 14 and the second storage chamber 22, for example, about 2 mL. Therefore, the sum of the volume of the second containment chamber 22 and the volume of the third containment chamber 27 (for example, about 7 mL) is smaller than the volume of the first containment chamber 14 (for example, about 20 mL).

The second connecting portion 26 connects the second accommodating portion 12 and the third accommodating portion 13 at the upper portion in the vertical direction 53. The second connecting portion 26 is formed by, for example, two resin sheets constituting the container 10 being brought into close contact with each other. The length of the outer shape of the second connecting portion 26 in the front-rear direction 51 is shorter than the length in the vertical direction 53. That is, the second connecting portion 26 has a flat shape in the front-rear direction 51.

The second connecting portion 26 is provided with communication passages 29 and 30 extending in the left-right direction 52. The communication passages 29 and 30 are arranged so as to be separated from each other in the vertical direction 53, and each communicates the second accommodation chamber 22 and the third accommodation chamber 27. The communication passages 29 and 30 are arranged at positions biased upward from the center of the outer shape of the second accommodating portion 12 and the third accommodating portion 13 in the vertical direction 53. Further, the communication passages 29 and 30 are opened at the ends of the second accommodation chamber 22 and the third accommodation chamber 27 in the left-right direction 52 at the position where the length of the left-right direction 52 is maximized (upper portion).

The third storage chamber 27 is provided with a sample collection port 31. The sample collection port 31 is provided at the lower end of the third storage chamber 27 in the vertical direction 53. The lower end of the sample collection port 31 is sealed and does not open to the outside. When the sample collection port 31 is used, the lower end side of the sample collection port 31 is cut and opened. A cylindrical stopper 37 is liquid-tightly inserted into the sample collection port 31. Since the structure of the stopper 37 is the same as that of the stopper 35, detailed description thereof will be omitted here. The sample collection port 31 is used to drain the cell suspension 40 stored in the third containment chamber 27.

[Sealing device 60]
The sealing device 60 is an impulse type heat sealer. As shown in FIG. 9, the sealing device 60 has a first pressing portion 61 provided in a lower frame (not shown) and a second pressing portion 62 provided in an upper frame (not shown). Although not shown in detail in each drawing, the upper frame is provided so as to be detachable from the lower frame, and the first connecting portion 21 or the second connecting portion of the container 10 supported along the lower frame is provided. The upper frame is lowered with respect to 26, and the first connecting portion 21 or the second connecting portion 26 is sandwiched between the first pressing portion 61 and the second pressing portion 62 arranged so as to face each other in the vertical direction 55. By heating, the communication passages 23, 24 or the communication passages 29, 30 are sealed by heat welding.

As shown in FIG. 9, the first pressing portion 61 includes a first heater portion 63, a first protective cover 64, and a first transformer 65. The first heater unit 63 is electrically connected to the first transformer 65 and is heated by the electric power supplied from the first transformer 65. The first protective cover 64 covers the upper part of the first heater portion 63. The second pressing portion 62 has a second heater portion 66, a second protective cover 67, and a second transformer 68. The second heater unit 66 is electrically connected to the second transformer 68 and is heated by the electric power supplied from the second transformer 68. The second protective cover 67 covers the lower part of the second heater portion 66.

Since the first pressing portion 61 and the second pressing portion 62 have the same structure except that the arrangement in the vertical direction 55 is different, a detailed configuration will be described below by taking the first pressing portion 61 as an example. The sealing device 60 has a first pressing portion 61 and a second pressing portion 62, but the sealing device according to the present invention is a sealing device provided with only one of the first pressing portion 61 and the second pressing portion 62. May be realized.

As shown in FIG. 10, the first heater unit 63 extends longer in the left-right direction 57 of the front-rear direction 56 and the left-right direction 57 orthogonal to the up-down direction 55. The length of the first heater unit 63 along the left-right direction 57 may be sufficient to seal the communication passages 23, 24, 29, and 30 of the container 10.

The first heater unit 63 has a heater main body 70 arranged in the center of the front-rear direction 56, and heat insulating materials 71 and 72 arranged on both sides of the heater main body 70 in the front-rear direction 56, respectively. The heater main body 70 is composed of, for example, a braid formed by assembling a plurality of thread-like nichrome wires, and the braid extends in the left-right direction 57 with a constant length along the front-rear direction 56. The length of the heater body 70 along the front-rear direction 56 is sufficiently shorter than the length of the container 10 along the first connecting portion 21 and the second connecting portion 26 in the left-right direction 52, and the passages 23, 24, 29, It is long enough to maintain the 30 seals. The heater body 70 corresponds to a heat generating member.

The heat insulating materials 71 and 72 are arranged adjacent to the heater main body 70. Examples of the materials of the heat insulating materials 71 and 72 include fluororesin (heat resistance and releasability), polytetrafluoroethylene, polyoxymethylene (POM), polypropylene (PP), polycarbonate (PC), polyamide resin, and polyethylene terephthalate. (PET) and the like. The length of the heat insulating materials 71 and 72 along the left-right direction 57 is the same as the length of the heater main body 70 along the left-right direction 57. The length of the heat insulating materials 71 and 72 along the front-rear direction 56 is slightly longer than the length of the heater main body 70 along the front-rear direction 56. The length of the heater body 70 and the heat insulating materials 71 and 72 as a whole along the front-rear direction 56 is shorter than the length of the container 10 along the first connecting portion 21 and the second connecting portion 26 in the left-right direction 52. The upper surfaces of the heat insulating materials 71 and 72 are substantially the same as the upper surface of the heater main body 70. Therefore, when the container 10 comes into contact with the upper surface of the heater main body 70, the container 10 also comes into contact with the upper surfaces of the heat insulating materials 71 and 72. The heat insulating materials 71 and 72 correspond to the first heat insulating material and the second heat insulating material.

Although detailed description using figures is omitted, the second heater unit 66 also has a configuration in which heat insulating materials are arranged adjacent to both sides of the heater main body, similarly to the first heater unit 63.

[Manufacturing method of cell filling container]
The cell-filled container is manufactured by filling the container 10 with the cell suspension 40 and sealing the first connecting portion 21 and the second connecting portion 26. The method for producing a cell-filled container is mainly classified into the following steps.
(1) Until the first containment chamber 14 is filled with the cell suspension 40, the cells enter the first containment chamber 14 without the cell suspension 40 flowing into the second containment chamber 22 through the passages 23 and 24. The first step of filling the suspension 40.
(2) The cell suspension 40 filled in the first storage chamber 14 through the communication passages 23 and 24 is allowed to flow into the second storage chamber 22, and the cell suspension filled in the first storage chamber 14 through the communication passages 29 and 30. The second step of flowing the turbid liquid 40 into the third storage chamber 27.
(3) In the second step, a step of sealing the communication passages 23 and 24 and the communication passages 29 and 30 of the container 10 filled with the cell suspension 40.

Examples of the cells filled in the container 10 include cells collected from a living body such as umbilical cord blood and stem cells, but the cells that can be cryopreserved using the container 10 are not limited thereto.

[First step]
As shown in FIG. 3, in the first step, the container 10 is maintained in a state where the second storage chamber 22 and the third storage chamber 27 are located above the first storage chamber 14 in the direction of gravity 54. Subsequently, the cell injection port 15 is connected to a decompression device such as an air pump or a syringe pump, and air is discharged from the first accommodating chamber 14, so that the first accommodating chamber 14 is decompressed.

Since the second accommodation chamber 22 and the third accommodation chamber 27 are communicated with the first accommodation chamber 14 through the communication passages 23, 24, 29, 30, the second accommodation chamber 22 and the third accommodation chamber 27 are also depressurized. However, decompression of the second containment chamber 22 and the third containment chamber 27 is not always necessary. Therefore, for example, only the first storage chamber 14 may be decompressed while the communication passages 23 and 24 are blocked by clips or the like.

After the first containment chamber 14 is decompressed, the cell infusion port 15 is disconnected from the decompression device and the cell suspension 40 is injected into the first containment chamber 14 through the cell infusion port 15 as shown in FIG. .. Since the first containment chamber 14 is depressurized, the cell suspension 40 is filled in the first containment chamber 14 even if the first containment chamber 14 is not in communication with the atmosphere.

The second accommodation chamber 22 and the third accommodation chamber 27 are above the first accommodation chamber 14 in the gravity direction 54, and the communication passages 23 and 24 are opened at the uppermost side of the first accommodation chamber 14 in the gravity direction 54. Therefore, the cell suspension 40 does not flow into the second storage chamber 22 and the third storage chamber 27 until the first storage chamber 14 is filled with the cell suspension 40. Therefore, air 41 remains in the second storage chamber 22 and the third storage chamber 27. After the first storage chamber 14 is filled with the cell suspension 40, the cell suspension 40 may flow from the first storage chamber 14 into the second storage chamber 22 and the third storage chamber 27.

[Second step]
As shown in FIG. 5, in the second step, the second accommodation chamber 22 and the third accommodation chamber 27 are maintained in a state of being located below the first accommodation chamber 14 in the direction of gravity 54. As a result, the cell suspension 40 filled in the first containment chamber 14 flows into the second containment chamber 22 through one of the communication passages 23 and 24. Further, the cell suspension 40 that has flowed into the second storage chamber 22 flows into the third storage chamber 27 through one of the communication passages 29 and 30. Since the communication passages 23 and 24 are open at the uppermost side of the second storage chamber 22 in the gravity direction 54, the air in the second storage chamber 22 smoothly reaches the first storage chamber 14 through one of the communication passages 23 and 24. It is discharged. Since the communication passages 29 and 30 are open at the uppermost side of the third accommodation chamber 27 in the gravity direction 54, the air in the third accommodation chamber 27 smoothly reaches the second accommodation chamber 22 through one of the communication passages 29 and 30. It is discharged.

The air 41 existing in the second accommodation chamber 22 and the third accommodation chamber 27 passes through the other of the communication passages 23 and 24 and the other of the communication passages 29 and 30. As a result, the air 41 existing in the second storage chamber 22 and the third storage chamber 27 is replaced with the cell suspension 40 and moves to the first storage chamber 14. In the communication passages 23 and 24 and the communication passages 29 and 30, the cell suspension 40 does not necessarily circulate on one side, and the air 41 does not necessarily circulate on the other side. For example, the cell suspension 40 and the air 41 may flow simultaneously or alternately in the passages 23, 24 and the passages 29, 30.

As shown in FIG. 6, after the container 10 has been allowed to stand until the second containment chamber 22 and the third containment chamber 27 are filled with the cell suspension 40, a decompression device is connected to the cell injection port 15. The air 41 remaining in the first storage chamber 14 is evacuated. When the air 41 is removed, the container 10 is deformed so that the volumes of the first storage chamber 14, the second storage chamber 22, and the third storage chamber 27 are bent by the amount corresponding to the remaining air 41. After that, the cell injection port 15 or the tube 18 is sealed by heat welding or the like. As a result, the first accommodation chamber 14, the second accommodation chamber 22, and the third accommodation chamber 27 become closed spaces.

Subsequently, as shown in FIG. 7, the communication passages 23 and 24 are sealed by heat welding in the first connecting portion 21, and the communication passages 29 and 30 are sealed by heat welding in the second connecting portion 26. To. These seals are performed using a sealing device 60.

The heat welding of the communication passages 23, 24 or the communication passages 29, 30 by the sealing device 60, that is, the seal is a step of pressing and closing the communication passages 23, 24 or the communication passages 29, 30 and the communication passages 23, 24 or the connection. It includes a step of heat-welding the pressed portions of the passages 29 and 30. Since the step of sealing the communication passages 23 and 24 and the step of sealing the communication passages 29 and 30 are the same, a detailed description will be given below by taking the step of sealing the communication passages 23 and 24 as an example.

As shown in FIG. 11, the container 10 is positioned with respect to the sealing device 60 so that the communication passages 23 and 24 are arranged in the longitudinal direction of the first heater portion 63, that is, in the left-right direction 57. Positioning of the container 10 may be realized by, for example, an operator holding the container 10 in his / her hand, or a jig for positioning the container 10 may be provided in the sealing device 60. The positioning of the container 10 with respect to the sealing device 60 is such that each of the first accommodating portion 11, the second accommodating portion 12, and the third accommodating portion 13 facing the front-rear direction 51 is along the horizontal direction. It is preferable to support the container 10 with a jig or the like so that the passages 23 and 24 are along the horizontal direction. As a result, the cell suspension 40 stored in the first storage portion 11, the second storage portion 12, and the third storage portion 13 is suppressed from moving through the communication passages 23 and 24, respectively. If the first accommodating portion 11 is located below the second accommodating portion 12 and the third accommodating portion 13, the cell suspension stored in the second accommodating portion 12 and the third accommodating portion 13 due to gravity. The originally planned amount of the turbid liquid 40 flowing into the first accommodating portion 11 and expanding the internal space of the first accommodating portion 11 so that the wall defining the internal space of the first accommodating portion 11 bends outward. A larger amount of the cell suspension 40 is stored in the first containing portion 11, and a smaller amount of the cell suspension 40 than originally planned is stored in the second containing portion 12 and the third containing portion 13. Although it will be stored, it is possible to prevent such a problem from occurring by positioning the container 10 as described above.

Further, in positioning the sealing device 60, each surface of the first accommodating portion 11, the second accommodating portion 12, and the third accommodating portion 13 facing the front-rear direction 51 is the first connecting portion 21 and the second connecting portion 26. Is sandwiched by a pair of flat plate-shaped support members without interfering with being sandwiched by the first heater portion 63 and the second heater portion 66, that is, without interfering with the first heater portion 63 and the second heater portion 66. It is preferable to have it. As will be described later, in the seal of the container 10, the communication passages 23 and 24 are sandwiched between the heat insulating materials 71 and 72 of the first heater portion 63 and the heat insulating material (not shown) of the second heater portion 66, whereby the continuous passages 23 and 24 are connected. The cell suspension 40 is extruded from a part 43 of the passages 23 and 24, and the first accommodating portion 11, the second accommodating portion 12, and the third accommodating portion 13 are sandwiched by a pair of flat plate-shaped support members and the like. As a result, it is possible to prevent the accommodating portion of any of the first accommodating portion 11, the second accommodating portion 12, and the third accommodating portion 13 from bending significantly. If the first accommodating portion 11, the second accommodating portion 12, and the third accommodating portion 13 are not sandwiched by the pair of support members, the cell suspension 40 is formed from a part 43 of the communication passages 23 and 24. Is extruded, so that one of the first accommodating portions 11, the second accommodating portion 12, and the third accommodating portion 13 that is flexible is flexed more than the other accommodating portions, and as a result, the extruded cell suspension is performed. The turbid liquid 40 may flow into one of the greatly bent accommodating portions, but the first accommodating portion 11, the second accommodating portion 12, and the third accommodating portion 13 are a pair of flat plate-shaped support members. By being sandwiched by such means, it is possible to prevent such a problem from occurring.

The first accommodating portion 11 and the second accommodating portion 12 of the container 10 positioned as described above are located at opposite positions of the first heater portion 63 in the front-rear direction 56, respectively. Although the second heater unit 66 is not shown in FIG. 11, the second heater unit 66 is located so as to face the first heater unit 63 in the vertical direction 55 (the direction perpendicular to the paper surface of FIG. 11). There is.

Subsequently, the second heater unit 66 is lowered and comes into contact with the first heater unit 63. As a result, the communication passages 23 and 24 of the container 10 are sandwiched and pressed between the heat insulating materials 71 and 72 of the first heater unit 63 and the heat insulating material (not shown) of the second heater unit 66, and the communication passages 23 are pressed. , 24 are crushed and closed. As shown in FIG. 12, the communication passages 23 and 24 are sandwiched between the heat insulating materials 71 and 72 of the first heater unit 63 and the heat insulating material (not shown) of the second heater unit 66. The cell suspension 40 moves from a part 43 of the communication passages 23 and 24 to the first accommodating portion 11 side or the second accommodating portion 12 side. Even between the heat insulating materials 71 and 72, a part 43 of the communication passages 23 and 24 is sandwiched between the heater body 70 of the first heater unit 63 and the heater body (not shown) of the second heater unit 66, so that heat insulation is provided. The cell suspension 40 existing between the materials 71 and 72 also moves to the first accommodating portion 11 side or the second accommodating portion 12 side.

With the cell suspension 40 moved from a part 43 of the passages 23 and 24, the first transformer 65 and the heater body (not shown) of the first heater unit 63 and the heater body 66 of the second heater unit 66 Power is supplied from the second transformer 68, respectively. As a result, the heater body 70 of the first heater unit 63 and the heater body (not shown) of the second heater unit 66 generate heat, and a part of 43 is approximately in the center of the front-rear direction 56, that is, the heater body of the first heater unit 63. The portions of the 70 and the second heater portion 66 in contact with the heater body (not shown) are heat-welded to form the sealing portion 42.

In addition, in order to secure a sufficient time for the cell suspension 40 to move from a part 43 of the communication passages 23 and 24 and to prevent the influence of heating on the cells as much as possible, the communication passages 23 and 24 are the first. After being sandwiched between the heat insulating materials 71 and 72 of the 1 heater unit 63 and the heat insulating material (not shown) of the second heater unit 66, the heater body 70 of the first heater unit 63 and the heater body of the second heater unit 66 It is preferable that a time lag of at least 0.1 to 1.0 second is set until power is supplied (not shown).

The time lag described above includes, for example, an operation for sandwiching the communication passages 23 and 24 between the heat insulating materials 71 and 72 of the first heater unit 63 and the heat insulating material (not shown) of the second heater unit 66, and the first operation. If it is a so-called two-action configuration in which the operation (for example, switch-on) for supplying electric power to the heater main body 70 of the heater unit 63 and the heater main body (not shown) of the second heater unit 66 is independent, the pinching is completed. It is realized by the control of disabling the switch-on for power supply from the detection of the above to the elapse of the time lag described above. Further, for example, an operation for sandwiching the communication passages 23 and 24 between the heat insulating materials 71 and 72 of the first heater unit 63 and the heat insulating material (not shown) of the second heater unit 66, and the operation of the first heater unit 63. The operation (for example, switch-on) for supplying electric power to the heater main body 70 and the second heater unit 66 (not shown) is, for example, in the direction in which the first heater unit 63 is brought into contact with the second heater unit. If it is a so-called one-action configuration that is completed by a series of operations to move, it is realized by setting the time lag described above from the input of the switch-on for power supply to the actual power supply. Will be done.

Although detailed description using figures is omitted, similarly, the communication passages 29 and 30 at the second connecting portion 26 are also heat-welded and sealed by the sealing device 60.

The sealing portion 42 makes each of the first storage chamber 14, the second storage chamber 22, and the third storage chamber 27 a closed space. After that, as shown in FIG. 8, in the first connecting portion 21 and the second connecting portion 26, the communication passages 23, 24 or the communication passages 29, 30 are cut along the heat-welded sealing portion 42, respectively. Then, the first accommodating portion 11, the second accommodating portion 12, and the third accommodating portion 13 are separated. If the second accommodating portion 12 and the third accommodating portion 13 are stored in the same place, the sealing portion 42 of the second connecting portion 26 is not cut, and the second accommodating portion 12 and the third accommodating portion 13 are used. It may remain one.

The first containment section 11, the second containing section 12, and the third containing section 13 filled with the cell suspension 40 as described above are the cell recovery port 17 or the sample when thawed and used. The collection ports 25 and 31 are opened to allow the cell suspension 40 to flow out of the first containment chamber 14, the second containment chamber 22, or the third containment chamber 27.

[Action and effect of this embodiment]
According to the present embodiment, after the first containment chamber 14 of the container 10 is filled with the cell suspension 40, the cell suspension 40 of the first containment chamber 14 is filled with the second containment chamber 22 and the third containment chamber 27. The cell suspension 40 in the same state as the cell suspension 40 stored in the first storage chamber 14 is stored as a test sample in the second storage chamber 22 and the third storage chamber 27.

In addition, using gravity, the cell suspension 40 is preferentially filled in the first storage chamber 14 in the first step, and in the second step, the first storage chamber 14, the second storage chamber 22, and the third storage chamber 14 are stored. Since the cell suspension 40 can flow into the chamber 27, each step can be easily realized.

Further, in the first step, since the cell suspension 40 is filled after depressurizing the first storage chamber 14, it is not necessary to communicate the first storage chamber 14 with the atmosphere.

Further, in the second step, after the second storage chamber 22 and the third storage chamber 27 are filled with the cell suspension 40, the air 41 remaining in the first storage chamber 14 is removed, so that the cells in the first storage chamber 14 are removed. It is possible to suppress the influence of the atmosphere on the suspension 40.

Further, in the second step, after filling the second storage chamber 22 and the third storage chamber 27 with the cell suspension 40, the communication passages 23, 24, 29, and 30 are sealed, so that the first storage chamber 14 , The second accommodation chamber 22, and the third accommodation chamber 27 can each be a closed space.

Further, since the volume of the second storage chamber 22 is smaller than the volume of the first storage chamber 14, the second storage chamber 22 is likely to be filled with the cell suspension 40 filled in the first storage chamber 14.

Further, in the second step, the passages 23, 24, 29, and 30 are pressed to block the passages 23, 24, 29, and 30, and the pressed portion is sealed by heat welding. 40 does not exist. Therefore, it is difficult to transfer the heat during heat welding to the cell suspension 40. Further, since the heater main body 70 heats and heat-welds while the communication passages 23, 24, 29, 30 are pressed and closed by the sealing device 60 heat insulating materials 71, 72, cells are found in the heat-welded portion. The suspension 40 does not exist, and it is difficult to transfer the heat during heat welding to the cell suspension 40.

Further, since the container 10 has the second accommodating portion 12 and the third accommodating portion 13, a plurality of test samples can be obtained.

Further, since the sum of the volume of the second accommodation chamber 22 and the volume of the third accommodation chamber 27 is smaller than the volume of the first accommodation chamber 14, the second accommodation chamber 22 and the third accommodation chamber 27 are the first accommodation chambers. It becomes easy to be filled with the liquid filled in 14.

Further, the length of the outer shape of the first connecting portion 21 and the second connecting portion 26 in the front-rear direction 51 is shorter than the length in the vertical direction 53, and the communication passages 23 and 24 are the first accommodating portion 11 and the second accommodating portion 12. The passages 29 and 30 are arranged at positions biased upward from the center of the outer shape of the outer shape in the vertical direction 53, and the communication passages 29 and 30 are above the center of the outer shape of the second housing portion 12 and the third housing portion 13 in the vertical direction 53. Since the positions are biased toward, it is easy to seal the passages 23, 24, 29, and 30 by heat welding or the like.

Further, the communication passages 23 and 24 are opened at the ends of the first accommodation chamber 14 and the second accommodation chamber 22 in the left-right direction 52 at the position where the length in the left-right direction 52 is maximum (upper portion). Since each of the communication passages 29 and 30 is open at the end of the second accommodation chamber 22 and the third accommodation chamber 27 in the left-right direction 52 at the position where the length of the left-right direction 52 is maximum (upper portion), the first When the 1st accommodating portion 11 is on the upper side and the 3rd accommodating portion 13 is on the lower side, air easily escapes from the 2nd accommodating chamber 22 and the 3rd accommodating chamber 27.

[Modification example]
In the above-described embodiment, in the first step, the second storage chamber 22 and the third storage chamber 27 are positioned above the first storage chamber 14 in the direction of gravity 54, so that the first storage chamber 14 becomes a cell. The cell suspension 40 is prevented from flowing into the second containment chamber 22 and the third containment chamber 27 until it is filled with the suspension 40, but without using gravity, for example, the communication passages 23 and 24 are clipped. By temporarily sealing the cells with, etc., the cell suspension 40 is prevented from flowing into the second storage chamber 22 and the third storage chamber 27 until the first storage chamber 14 is filled with the cell suspension 40. May be good.

Similarly, in the second step, the second containment chamber 22 and the third containment chamber 27 are located below the first containment chamber 14 in the direction of gravity 54, so that the cells are suspended in the first containment chamber 14. The liquid 40 flows into the second storage chamber 22 and the third storage chamber 27, but the first storage chamber 14 to the second storage chamber 14 to the second storage chamber 14 are stored by, for example, pressing the first storage portion 11 from the outside without using gravity. The cell suspension 40 may be allowed to flow into the chamber 22 and the third containment chamber 27.

Further, in the container 10, the cell suspension 40 as a test sample is stored in the second storage chamber 22 and the third storage chamber 27, but if a plurality of test samples are unnecessary, the third storage unit 13 is provided. Only the second accommodating portion 12 may be provided in the container 10 for storing the test sample. On the other hand, if three or more test samples are required, in addition to the second accommodating portion 12 and the third accommodating portion 13, a similar accommodating portion may be provided in the container 10 for storing the test sample.

Further, in the container 10, the position where the length of the left-right direction 52 is maximum in the first storage chamber 14, the second storage chamber 22, and the third storage chamber 27 is the upper portion in the vertical direction 53, but the horizontal direction 52 The position where the length of the maximum is maximum may be the lower portion of the vertical direction 53 or the central portion. For example, in the first accommodation chamber 14, the second accommodation chamber 22, and the third accommodation chamber 27, if the position where the length of the left-right direction 52 is maximum is the lower portion of the vertical direction 53, the respective communication passages 23, 24, The 29 and 30 are unevenly arranged in the lower portions of the first accommodating portion 11, the second accommodating portion 12, and the third accommodating portion 13.

Further, in the container 10, cell (sample) collection ports are provided under the first accommodating portion 11, the second accommodating portion 12, and the third accommodating portion 13, but the positions of these ports are appropriately changed. May be good.

Further, the sealing device 60 separately seals the communication passages 23 and 24 of the first connecting portion 21 and the communication passages 29 and 30 of the second connecting portion 26, but the first pressing portion 61 and the second pressing portion 62. The sealing device 60 may be configured so that two sets of the above can be provided so that the communication passages 23 and 24 of the first connecting portion 21 and the communication passages 29 and 30 of the second connecting portion 26 can be sealed at the same time. The communication passages 23 and 24 of the first connecting portion 21 of the container 10 and the communication passages 29 and 30 of the second connecting portion 26 may be sealed by a device other than the sealing device 60.

10 Container 11 1st storage 12 2nd storage 13 3rd storage 14 1st storage 15 Cell injection port 16 Reserve port 17 Cell recovery port 21 1st connecting 22 2nd storage 23, 24, 29, 30 Communication passages 25, 31 Sample collection port 26 2nd connecting part 27 3rd storage chamber 42 Sealing part 43 Part 60 Sealing device 70 Heater body (heating member)
71,72 Insulation material (1st insulation material, 2nd insulation material)

Claims (16)

A first containment compartment that partitions a first containment chamber having at least one port,
A second accommodating portion which is connected to the first accommodating portion via a first accommodating portion and which partitions a second accommodating chamber having at least one port, and a second accommodating portion.
A cell-filled container in which a liquid containing cells is filled in a container provided in the first connecting portion and having at least two communication passages connecting the first storage chamber and the second storage chamber. It is a method of manufacturing a cell-filled container to obtain
The first step of filling the first storage chamber with the liquid in a state where the liquid does not flow into the second storage chamber through the communication passage until the first storage chamber is filled with the liquid.
A method for producing a cell-filled container, comprising a second step of causing the liquid filled in the first storage chamber to flow into the second storage chamber through the communication passage. In the first step, the second storage chamber is positioned above the first storage chamber in the direction of gravity, and the first storage chamber is filled with the liquid.
In the second step, the second storage chamber is positioned below the first storage chamber in the direction of gravity, and the liquid filled in the first storage chamber is transferred to the second storage chamber through the communication passage. The method for producing a cell-filled container according to claim 1, wherein the inflow is performed. The method for producing a cell-filled container according to claim 1 or 2, wherein in the first step, at least the first storage chamber is depressurized and then the first storage chamber is filled with the liquid. The method for producing a cell-filled container according to any one of claims 1 to 3, wherein in the second step, after the second storage chamber is filled with the liquid, the gas remaining in the first storage chamber is removed. The method for producing a cell-filled container according to any one of claims 1 to 4, wherein in the second step, after the second storage chamber is filled with the liquid, the communication passage of the first connecting portion is sealed. The method for producing a cell-filled container according to claim 5, wherein in the second step, the communication passage of the first connecting portion is pressed and closed, and the pressed portion is heat-welded and sealed. Using a sealing member in which a heat generating member is arranged between the first heat insulating material and the second heat insulating material,
The first heat insulating material and the second heat insulating material are arranged and pressed on the first accommodating portion side and the second accommodating portion side of the communication passage of the first connecting portion.
The method for producing a cell-filled container according to claim 6, wherein heat welding is performed between the first heat insulating material and the second heat insulating material by the heat generating member. The method for producing a cell-filled container according to any one of claims 1 to 7, wherein the volume of the second storage chamber is smaller than the volume of the first storage chamber. The container is connected to the opposite side of the first accommodating portion via the second accommodating portion and the second accommodating portion, and has a third accommodating portion for partitioning a third accommodating chamber having at least one port. It is provided in the second connecting portion, and further includes at least two communication passages that communicate the second accommodating chamber and the third accommodating chamber.
The method for producing a cell-filled container according to any one of claims 1 to 8, wherein in the second step, the liquid filled in the first storage chamber is allowed to flow into the third storage chamber. The method for producing a cell-filled container according to claim 9, wherein the sum of the volume of the second storage chamber and the volume of the third storage chamber is smaller than the volume of the first storage chamber. A first containment compartment that partitions the first containment chamber having at least a cell injection port,
A second accommodating portion which is connected to the first accommodating portion via a first accommodating portion and which partitions a second accommodating chamber having at least one port, and a second accommodating portion.
A third accommodating portion which is connected to the opposite side of the first accommodating portion via the second accommodating portion and the second accommodating portion and which partitions a third accommodating chamber having at least one port, and a third accommodating portion.
At least two communication passages provided in the first connecting portion and communicating the first accommodation chamber and the second accommodation chamber, and
It is provided in the second connecting portion, and includes at least two communication passages that communicate the second accommodating chamber and the third accommodating chamber.
Each of the communication passages provided in the first connecting portion and each of the communication passages provided in the second connecting portion are along the first direction in which the first accommodating portion and the second accommodating portion are lined up. It is extended and parallel to the second direction orthogonal to the first direction.
In the first accommodating portion, the second accommodating portion, and the third accommodating portion, the length of the outer shape in the first direction and the outer shape in the third direction orthogonal to the second direction is larger than the length of the outer shape in the second direction. Each of the passages provided in the first connecting portion and the connecting passages provided in the second connecting portion are short, and the first accommodating portion, the second accommodating portion, and the third accommodating portion are provided. It is arranged more biased to one of the second directions than the center position of the outer shape of the second direction.
The length of the first accommodating portion in one of the second directions is the maximum, and the length of the other portion in the second direction is gradually shortened in the first direction. Cell filling container. The cell injection port is located in one of the second directions in the first accommodating portion.
The cell filling container according to claim 11, wherein the first storage portion has a cell recovery port on the other side in the second direction. The cell filling container according to claim 12, wherein the first accommodating portion has a hanging hole in one of the second directions. The length of the second accommodating portion in one of the second directions is the maximum in the first direction, and the length of the other portion in the second direction is gradually shortened in the first direction. The cell filling container according to any one of claims 11 to 13. Each communication passage provided in the first connecting portion and each communication passage provided in the second connecting portion communicate with the second storage chamber or the third storage chamber by the cell filling container. Claim that the first accommodating portion is located on the upper side in the gravity direction and the third accommodating portion is located on the lower side in the gravity direction, and is located on the uppermost side in the gravity direction in the second accommodating chamber or the third accommodating chamber. The cell filling container according to any one of 11 to 14. The cell filling container according to any one of claims 11 to 15, wherein the sum of the volume of the second storage chamber and the volume of the third storage chamber is smaller than the volume of the first storage chamber.

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