JP6767043B2 - Blood separator - Google Patents

Description

The present invention relates to a blood separator that separates blood into plasma and blood cells.

Normally, it takes several mL or more to collect blood for blood diagnosis, which imposes a heavy physical burden on the person to be collected. In addition, when a large and expensive analyzer is required for blood diagnosis, in a small medical institution that does not have such an apparatus, the analysis is outsourced to a specialized clinical laboratory to obtain the diagnosis result. It takes a few days. In order to solve this situation, research and development are being actively carried out to build a blood diagnostic function in a small device. If this is realized, it will be possible to reduce the amount of blood collected and the time required to obtain a diagnosis result, and it will be possible to apply it to point-of-care diagnosis including bedside diagnosis and home diagnosis.

By the way, when the target of blood diagnosis is a protein in plasma, blood separation is required to separate the collected blood into plasma and blood cells. If blood can be separated with a small device, it can be applied to a technique for constructing a blood diagnostic function in a small device.
Patent Documents 1 and 2 describe a method in which a small device is attached to a centrifuge and blood is separated by using centrifugal force. This method has problems in that the centrifuge used is large and that it is necessary to carry the centrifuge.

As a specific example of performing blood separation without using a centrifuge, Patent Document 3 describes a method of separating blood using a filter. In this method, there are problems such as hemolysis due to physical load on blood cells and clogging of the filter. Dielectrophoresis can be considered as a method that does not use a centrifuge or a filter, but it is necessary to dilute blood, and there is a concern that the application of voltage may affect plasma components.

As a simpler blood separation method, there is a method using blood cell sedimentation due to gravity. Blood cell sedimentation is completed in a shorter time when the flow path filled with blood is arranged diagonally with respect to the vertical as compared with the case where the flow path filled with blood is arranged vertically. This is because when the flow path is arranged diagonally with respect to the vertical, the sedimentation of blood cells and the rise of plasma do not interfere with each other, and this blood cell sedimentation promoting effect is called a boycott effect. Patent Documents 4 and 5 describe specific examples of separating blood by utilizing the boycott effect.

Japanese Unexamined Patent Publication No. 2003-83858 Japanese Unexamined Patent Publication No. 2006-52950 Japanese Unexamined Patent Publication No. 2003-270239 Japanese Unexamined Patent Publication No. 2008-82896 Japanese Unexamined Patent Publication No. 2008-82897

However, in the examples described in Patent Documents 4 and 5, there is a problem that it takes several minutes or more to separate the blood because the blood is separated only by the boycott effect. If it takes a long time to separate blood, it cannot be applied to urgent medical diagnosis. If the blood separation time can be shortened, the diagnostic efficiency can be improved, and as a result, the diagnostic time and the diagnostic cost can be reduced.
The present invention has been made in view of such circumstances, and an object of the present invention is to provide a blood separator capable of shortening the time required for blood separation utilizing the boycott effect.

The blood separator according to the present invention according to the above object is a blood separator that separates blood into plasma and blood cells by blood cell sedimentation, and is a blood inlet and a first tube into which blood introduced from the inlet flows. a road, and a blood-receiving portion blood having passed through the first conduit is supplied, it is connected to said first conduit, a second, generally filled with blood that has flowed from the first duct The second pipeline has a connecting region in which one end is connected to the first pipeline and is inclined downward toward the other end, and the first pipeline is the said. The downward basin portion that communicates with the connection region and allows blood toward the blood supply portion to flow downward is provided, and the downward direction of the first pipeline is provided with the entire second conduit filled with blood. flow occurs in blood toward the blood supply target portion at the basin portion, sedimentation of blood cells in the connection region of the second conduit by the blood flow of the lower-facing basin portion is accelerated, separated plasma is the connection Accumulate in the area.

In the blood separator according to the present invention according to the above object, the downstream end of the first pipeline is located at a position lower than the connection point of the first pipeline to the second pipeline, and the above. Connected to the blood supply section, the blood supply section is hydrophilic, is formed by opening, stores blood from the first line, and is the second of the first line. It is preferable that the sum of the volume on the downstream side of the connection point to the pipeline and the volume of the blood supply portion is larger than the volume of the second pipeline.

In the blood separator according to the present invention according to the above object, it is preferable that the upstream end of the first pipeline is connected to the introduction port.

In the blood separator according to the present invention according to the above object, it is preferable that the first pipeline is linear and vertically arranged.

In the blood separator according to the present invention according to the above object, an exhaust port for exhausting the gas existing in the second pipeline is connected to the downstream end of the second pipeline, and the exhaust port is hydrophobic. It is preferable to have sex.

In the blood separator according to the present invention according to the above object, the first and second conduits have hydrophilicity, and the blood introduced into the introduction port is subjected to the first and second ducts by capillary force. It is preferable to flow through the pipeline.

In the blood separator according to the present invention, the second pipeline has a connecting region in which one end is connected to the first pipeline and is inclined downward toward the other end, and the first pipeline has a connecting region. Since it is provided with a downward basin portion in which blood flowing toward the blood supply portion flows downward, the downward blood flow in the downward basin portion promotes blood cell sedimentation in the connection region, and blood utilizing the boycott effect. The time required for separation can be shortened.

(A) and (B) are a front view and a plan view of the blood separator according to the first embodiment of the present invention, respectively. (A), (B), and (C) are explanatory views showing the state of blood in the blood separator, respectively. (A) to (G) are explanatory views which show the manufacturing process of the blood separator which concerns on 1st Embodiment of this invention, respectively. (A) and (B) are a front view of the blood separator according to the second embodiment of the present invention and an explanatory view showing the state of blood in the blood separator, respectively. It is a front view of the blood separator which concerns on a comparative example. It is a photograph which imaged the state with the passage of time of separation of blood plasma and blood cell of the blood separator which concerns on Example. This is an experimental result of measuring the expansion of the separated plasma accumulated area with the passage of time.

Subsequently, an embodiment embodying the present invention will be described with reference to the attached drawings, and the present invention will be understood.
As shown in FIGS. 1 (A), 1 (B), 2 (A), (B), and (C), the blood separator 10 according to the first embodiment of the present invention is a blood B inlet. 11, the blood supply section 12 (first line) into which the blood B introduced from the introduction port 11 flows in, and the blood supply unit 13 to which the blood B passing through the line 12 is supplied, and the whole line. It is provided with a conduit 14 (second conduit) filled with blood B flowing in from 12, and blood B is separated into plasma P and blood cells C by blood cell sedimentation. The details will be described below.

As shown in FIGS. 1A and 1B, the blood separator 10 has a translucent plate 15 and a translucent plate 16 having a front side closely attached to the back side of the plate 15. It has.
The plate material 15 is provided with linear grooves 17, 18 and 19 formed on the back surface side, and through holes 20, 21 and 22 penetrating from the front side to the back surface side of the plate material 15.
One ends (upper ends) of the grooves 17 and 19 are connected to each other, and the entire grooves 17 and 19 form an inverted V-shaped groove whose center is bent and protrudes upward. One end (upper end) of the groove 18 is connected to a bent portion of an inverted V-shaped groove composed of the grooves 17 and 19, and is formed linearly toward the lower end directly below the one end.

The through holes 20, 21, and 22 are connected to the other ends (lower ends) of the grooves 17, 18, and 19, respectively.
The plate material 16 closes the back surface side of the plate material 15 of the groove 17 to form the pipeline 14, and closes the back surface side of the plate material 15 of the grooves 18 and 19 to form the pipeline 12, and the through holes 20, 21 and 22. The back surface side of the plate material 15 is closed to form an air exhaust port 23, a blood supply unit 13, and a blood introduction port 11, respectively.

The diameter and depth (thickness of the plate material 15) of the blood supply portion 13, the introduction port 11 and the exhaust port 23 are the same. In the present embodiment, the diameters of the blood supply unit 13, the introduction port 11 and the exhaust port 23 are 1 to 3 mm, and the depth is 1 to 3 mm.
The widths of the pipelines 12 and 14 are equal, and the depths of the pipelines 12 and 14 are equal. In the present embodiment, the widths of the pipelines 12 and 14 are 100 to 400 μm, and the depths of the pipelines 12 and 14 are 50 to 300 μm.

The plate materials 15 and 16 are made of a hydrophobic COC (Cyclo olefin copolymer), and the regions corresponding to the pipelines 12 and 14, the blood supply unit 13 and the introduction port 11 are hydrophilized (this). In the embodiment, a treatment for modifying phosphorylcholine) is performed. Therefore, the pipelines 12 and 14, the blood supply unit 13 and the introduction port 11 have hydrophilicity, and the exhaust port 23 has hydrophobicity.

One end (upstream end) and the other end (downstream end) of the pipeline 12 are connected to the introduction port 11 and the blood supply unit 13, respectively. The pipeline 12 has a straight line from one end to a portion where the pipeline 12 is connected to the pipeline 14 (hereinafter, also referred to as a “connection portion of the pipeline 12 to the pipeline 14”), and is a pipeline of the pipeline 12. The connection point to the 14 is arranged diagonally above one end of the pipeline 12, and the connection point to the other end of the pipeline 12 to the pipeline 14 is linearly and vertically arranged. That is, the pipeline 12 is bent at a position where the pipeline 12 is connected to the pipeline 14.
Therefore, the other end of the pipeline 12 connected to the blood supply portion 13 is located at a position lower than the connection point of the pipeline 12 to the pipeline 14.

The blood supply unit 13 is formed by opening the front side of the plate material 15 and is designed to exhaust air. The pipeline 14 is linear, and one end (upstream end) connected to the pipeline 12 is located diagonally above the other end (downstream end) connected to the exhaust port 23. Therefore, the pipeline 14 has a connection region 24 in which one end (upstream end) is connected to the pipeline 12 and is inclined diagonally downward (that is, downward) toward the other end (downstream end). Since the exhaust port 23 is connected to the pipeline 14, the air in the pipeline 14 can be exhausted.

When blood B is introduced into the introduction port 11, the blood B introduced into the introduction port 11 is connected from one end of the pipeline 12 to the pipeline 14 of the pipeline 12 as shown in FIG. 2 (A). After flowing toward the portion and reaching the connecting portion of the conduit 12 to the conduit 14, the blood B flowing into the conduit 14 and the blood B flowing through the conduit 12 toward the blood supply portion 13 are separated. At this time, the air (that is, gas) existing in the pipeline 14 is mainly exhausted from the exhaust port 23, and the air existing in the pipeline 12 is exhausted from the exhaust port 23 and the blood supply unit 13. ..

After the blood B reaches the connection point of the pipeline 12 to the pipeline 14, the region near the connection region 24 of the pipeline 12 (the region in contact with the connection region 24 of the pipeline 12) is changed from the pipeline 12 to the pipeline. A flow of blood B toward 14 and a flow of blood B moving downward toward the blood supply unit 13 occur.
Hereinafter, in the vicinity of the connection region 24 of the pipeline 12, the region of the pipeline 12 in which the flow of blood B traveling downward toward the blood supply portion 13 is generated is referred to as the downward basin portion 25. Therefore, the pipeline 12 communicates with the connection region 24, and the downward basin portion 25 in which the blood B toward the blood supply portion 13 flows directly below (an example below, the “downward” here includes diagonally below). Will be provided. That is, the connection region 24 and the downward basin portion 25 are connected so that blood B can circulate.

Blood B flows through the conduits 12 and 14 by capillary force. The blood B that has flowed through the pipeline 12 and reached the downstream end of the pipeline 12 flows into the blood supply unit 13 and is stored in the blood supply unit 13. Since the blood B is held in the conduit 12 by the capillary force, the blood B does not flow out from the introduction port 11 or the blood supply unit 13.
Since the exhaust port 23 is hydrophobic, blood B in the pipeline 14 does not substantially flow into the exhaust port 23. Therefore, after the blood B flowing through the pipeline 14 reaches the lower end of the pipeline 14 and the entire inside of the pipeline 14 is filled with the blood B, as shown in FIG. 2B, from the pipeline 12 The inflow of blood B into the conduit 14 is substantially eliminated (at the downward basin 25, the flow of blood B from the conduit 12 to the conduit 14 is practically eliminated).

In the present embodiment, the total of the volume on the downstream side of the connection point of the pipeline 12 to the pipeline 14 and the volume of the blood supply unit 13 is larger than the volume of the pipeline 14. Therefore, when the entire inside of the pipe line 14 is filled with the blood B, at least the blood supply unit 13 has a space for storing the blood B.
Therefore, after the entire inside of the pipe line 14 is filled with the blood B, in the downward basin portion 25, substantially only the flow of the blood B toward the blood supply portion 13 occurs. The flow of blood B toward the blood supply section 13 in the downward basin section 25 continues until the blood supply section 13 is filled with the blood B.

Since the connecting region 24 is inclined downward, in the conduit 14 filled with blood B, the connecting region 24 is the blood B due to the boycott effect, as shown in FIGS. 2 (B) and 2 (C). Separation into plasma P and blood cells C occurs. Moreover, in the connection region 24, the sedimentation of blood cells C is promoted by the flow of blood B directly below the downward basin portion 25. Therefore, in the present embodiment, the downward basin portion 25 is directed toward the blood supply portion 13. The separation of blood B into plasma P and blood cells C is performed more rapidly in the connection region 24 than in the case where there is no flow of blood B directly below. Then, the separated plasma P accumulates in the connecting region 24.

In the present embodiment, since there is substantially no inflow of blood B from the pipeline 14 to the exhaust port 23, blood B is introduced into the introduction port 11 as compared with the case where blood B flows into the exhaust port 23. The time from then until the flow of blood B in the conduit 14 is stopped is short. By stopping the flow of blood B in the pipe line 14, the sedimentation of blood cells C proceeds stably without being disturbed. Therefore, in the present embodiment, the time until the sedimentation of blood cells C progresses stably is shortened. It is possible.

In the connecting region 24, after the volume of the portion where the separated plasma P is stored becomes a predetermined value or more, light is applied to the connecting region 24, and the absorptiometry of the plasma P accumulated in the connecting region 24 is targeted. Measurements are made by law. By the absorptiometry for plasma P, for example, AST (GOT), ALT (GPT), γ-GT (γ-GTP), neutral fat, HDL cholesterol, LDL cholesterol, bilirubin, albumin, glucose, HbA1c, The value of uric acid can be determined.
The measurement for the separated plasma P is not limited to the absorptiometry method. For example, an electrode is provided in advance in the connection region 24, and electrochemical for the plasma P accumulated in the connection region 24. Target measurement may be performed.

The blood separator 10 can be manufactured by using a nanoimprint apparatus. Hereinafter, a method for manufacturing the blood separator 10 will be described with reference to FIGS. 3 (A) to 3 (G).
First, by plasma CVD (Chemical Vapor Exposure), as shown in FIG. 3A, SiN films 27 and 27a having a film thickness of 1 μm are provided on the upper surface and the lower surface of the Si substrate 26, respectively, and the resist 28 is applied to the SiN film 27. Then, as shown in FIG. 3B, the mask 29 on which the predetermined pattern is drawn is UV-exposed from above in a state of being arranged above the Si substrate 26 to develop.

Next, the SiN film 27 is etched so that the film piece 30 of the SiN film 27 and the resist 28 is left only in a predetermined region of the SiN film 27 as shown in FIG. 3C, and the film piece 30 of the resist 28 is left in the etching process. As shown in (D), the region where the film piece 30 of the Si substrate 26 is not provided above is melted, and the protruding region 31 is provided in the region where the film piece 30 of the Si substrate 26 is provided to form the Si substrate 26. The mold 32 is formed on the basis. The protruding area 31 corresponds to the groove 17 and the like.

Then, using the nanoimprint device, as shown in FIGS. 3 (E) and 3 (F), the groove 17 and the like are transferred to the back surface side of the COC substrate 33 based on the mold 32, and as shown in FIG. 3 (G). After irradiating the back side of the COC substrate 33 and the front side of the raw COC substrate 34 with VUV (Vacum ultraviolet) light, the front side of the COC substrate 34 is brought into close contact with the back side of the COC substrate 33. Then, by heating and pressurizing, the back side of the COC substrate 33 and the front side of the COC substrate 34 are joined by a covalent bond, and hydrophilization treatment is performed at a predetermined location, whereby a blood separator provided with a conduit 14 or the like 10 is manufactured.

As described above, in the blood separator 10, the line 12 (that is, the first line) is bent, but the blood separator 10 is not limited to this. Hereinafter, the blood separator 40 having a linear first conduit will be described with reference to FIGS. 4 (A) and 4 (B). The same configuration as that of the blood separator 10 is designated by the same reference numerals as that of the blood separator 10 and detailed description thereof will be omitted.

As shown in FIG. 4A, the blood separator 40 according to the second embodiment of the present invention closely fixes the back surface side of the plate material 41 having a groove or the like formed on the back surface side and the front side of the plate material 42. It is connected to the introduction port 11, the pipeline 43 (first pipeline) into which the blood B introduced from the introduction port 11 flows in, the blood supply unit 13, and the pipeline 43. Therefore, it is provided with a pipeline 44 (second pipeline) which is entirely filled with blood B flowing from the pipeline 43.

The pipeline 43 is linear and vertically arranged, and the upper end (upstream end) and the lower end (downstream end) are connected to the introduction port 11 and the blood supply portion 13, respectively. The pipeline 44 is linear and is arranged so as to be inclined with respect to the pipeline 43. The upper end (upstream end) is connected to the center in the longitudinal direction of the pipeline 43, and the lower end (downstream end) is connected to the exhaust port 23. ing. Therefore, the downstream end of the pipeline 43 is arranged at a position lower than the connecting position of the pipelines 43 and 44, and the pipeline 44 has a connection region in which one end is connected to the pipeline 43 and is inclined downward toward the other end. 45 exists, and the pipeline 43 is provided with a downward basin portion 46 in which the blood B toward the blood supply portion 13 flows directly below in the region near the connection region 45.

In the blood separator 40, similarly to the blood separator 10, the sum of the volume on the downstream side of the connecting position of the pipelines 43 and 44 of the pipeline 43 and the volume of the blood supply unit 13 is larger than the volume of the pipeline 44. .. Therefore, the blood B introduced from the introduction port 11 and flowing through the pipe line 43 reaches the lower end of the pipe line 44, and as shown in FIG. 4 (B), the entire inside of the pipe line 44 is filled with the blood B, and then the pipe. The inflow of blood B from the route 43 to the pipeline 44 is substantially stopped, and the inflow of blood B from the conduit 43 to the blood supply unit 13 is continued.

By maintaining this state, the boycott effect in the pipeline 44 and the influence of the flow of blood B directly below in the downward basin 46 cause the plasma cell C to settle in the contiguous zone 45, and as a result, The separated plasma P collects in the contiguous zone 45.
In the state where the blood line 44 (line line 14) is filled with blood B, the blood separator 40 allows blood B to proceed substantially only directly below from the upstream end to the downstream end of the line line 43, whereas the blood The separator 10 advances the blood B diagonally upward from the upstream end of the pipeline 12 toward the connecting position of the pipelines 12 and 14, and then changes the traveling direction and proceeds directly below. Therefore, the downward basin portion 46 of the blood separator 40 has a higher flow velocity of blood B than the downward basin portion 25 of the blood separator 10, and the connection region 45 of the blood separator 40 is the connection region 24 of the blood separator 10. It is possible to promote the sedimentation of blood cells C as compared with.

Next, an experiment conducted to confirm the action and effect of the present invention will be described.
In the experiment, the blood separator 10 (Example) shown in FIGS. 1 (A) and 1 (B) and the blood separator 50 (Comparative Example) shown in FIG. 5 were used.
As shown in FIG. 5, the blood separator 50 is formed by closely fixing two plates 51 and 52, and is a vertically arranged pipe having a blood inlet 53 and a lower end connected to the inlet 53. It includes a road 54, an inverted V-shaped pipe line 55 connected to a portion where the upper end of the pipe line 54 protrudes upward and bends, and exhaust ports 56 and 57 connected to both ends of the pipe line 55, respectively. ..

The introduction port 53 and the pipelines 54 and 55 are hydrophilic, and the exhaust ports 56 and 57 are hydrophobic. The flow path composed of the entire lines 54 and 55 of the blood separator 50 has the same length, cross-sectional area, and arrangement as the flow path composed of the entire lines 12 and 14 of the blood separator 10.
In the blood separator 50, the blood introduced into the introduction port 53 flows through the pipe line 54 and reaches the pipe line 55, and then flows through the pipe line 55 toward the exhaust ports 56 and 57. In the state where the entire pipes 54 and 55 are filled with blood, plasma separated from the blood cells accumulates in the vicinity of the bent portion of the pipe 55 due to the sedimentation of blood cells due to the boycott effect.

Regarding the blood separator 10, the time during which the blood was filled in the entire conduit 14 was set to 0 seconds, and the region where the plasma separated from the blood cells accumulated in the connecting region 24 was observed to expand with the passage of time. Specifically, the length in the direction marked “Sedimentation distance” in FIG. 6 of the relevant area was measured every 10 seconds (FIG. 6 shows 0 seconds, 30 seconds, and 60 seconds of the relevant area). Images (photographs) of the state at seconds and 90 seconds, respectively).

Regarding the blood separator 50, the time during which blood is filled in the entire pipes 54 and 55 is set to 0 seconds, and the region in which the plasma separated from the blood cells collects in the vicinity of the bent portion of the pipe 55 is targeted. The length in the vertical direction was measured every 10 seconds.
The measurement results of the blood separators 10 and 50 are shown in the graph of FIG. 7 as "Examples" and "Comparative Examples", respectively. From the measurement results, it was confirmed that the blood separator 10 expands the region of plasma separated from the blood cells faster than the blood separator 50.

Although the embodiments of the present invention have been described above, the present invention is not limited to the above-described embodiments, and all changes in conditions that do not deviate from the gist are within the scope of the present invention.
For example, in the downward basin portion of the first pipeline, the blood directed to the blood supply portion may flow diagonally downward instead of directly downward.
Further, the blood supply section may be any device as long as it allows the blood to flow in the first conduit toward the blood supply section after the entire second conduit is filled with blood, and the blood is not necessarily supplied. It doesn't have to be something to store. For example, the blood supply unit has an absorber that absorbs blood at the downstream end of the first pipeline, or has a drive source such as a pump to supply blood from the downstream end of the first pipeline. It may be sucked up.

The second pipeline need only have a connection region in which one end is connected to the first pipeline and is inclined downward toward the other end, and the entire second pipeline does not have to be linear.
Further, it is not necessary to fill the first and second conduits with blood by capillary force, and the driving source of the pump may be used to fill the first and second conduits with blood. When the first and second conduits are filled with blood by a driving source, the first and second conduits do not have to be hydrophilic.

10: Blood separator, 11: Introduction port, 12: Pipe line, 13: Blood supply part, 14: Pipe line, 15, 16: Plate material, 17, 18, 19: Groove, 20, 21, 22: Through hole , 23: Exhaust port, 24: Connection area, 25: Downward basin, 26: Si substrate, 27, 27a: SiN film, 28: Resist, 29: Mask, 30: Film piece, 31: Projection area, 32: Mold , 33, 34: COC substrate, 40: Blood separator, 41, 42: Plate material, 43, 44: Pipe line, 45: Connection area, 46: Downward basin, 50: Blood separator, 51, 52: Plate material, 53: Introduction port, 54, 55: Pipe line, 56, 57: Exhaust port, B: Blood, C: Blood cell, P: Plasma

Claims (6)

In a blood separator that separates blood into plasma and blood cells by blood cell sedimentation,
Blood inlet and
The first pipeline into which the blood introduced from the introduction port flows in, and
A blood supply unit to which blood that has passed through the first pipeline is supplied, and
It comprises a second line that is connected to the first line and is entirely filled with blood flowing in from the first line.
The second pipeline has a connection region in which one end is connected to the first pipeline and is inclined downward toward the other end.
The first pipeline includes a downward basin portion that communicates with the connection region and allows blood flowing downward toward the blood supply portion.
In a state where the entire second pipeline is filled with blood, a blood flow toward the blood supply portion is generated in the downward basin portion of the first pipeline, and the blood flow in the downward basin portion causes the blood flow. blood separator said sedimentation of blood cells in the connection area of the second conduit is accelerated, separated plasma is equal to or accumulating in the connection region. In the blood separator according to claim 1, the downstream end of the first pipeline is located at a position lower than the connection point of the first pipeline to the second pipeline, and the blood cover. Connected to the supply section, the blood supply section is hydrophilic, is formed by opening, stores blood from the first line, and is connected to the second line of the first line. A blood separator characterized in that the sum of the volume on the downstream side of the connection point to the blood supply portion and the volume of the blood supply portion is larger than the volume of the second pipeline. The blood separator according to claim 1 or 2, wherein the first conduit has an upstream end connected to the introduction port. The blood separator according to any one of claims 1 to 3, wherein the first conduit is linear and vertically arranged. In the blood separator according to any one of claims 1 to 4, an exhaust port for exhausting a gas existing in the second pipeline is connected to a downstream end of the second pipeline. A blood separator characterized in that the exhaust port has hydrophobicity. In the blood separator according to any one of claims 1 to 5, the first and second conduits have hydrophilicity, and the blood introduced into the introduction port has the first one due to capillary force. , A blood separator characterized by flowing through a second conduit.