JP6120529B2 - 鶏用飼料及び鶏の飼育方法 - Google Patents
鶏用飼料及び鶏の飼育方法 Download PDFInfo
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- JP6120529B2 JP6120529B2 JP2012248248A JP2012248248A JP6120529B2 JP 6120529 B2 JP6120529 B2 JP 6120529B2 JP 2012248248 A JP2012248248 A JP 2012248248A JP 2012248248 A JP2012248248 A JP 2012248248A JP 6120529 B2 JP6120529 B2 JP 6120529B2
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- Prior art keywords
- chicken
- feed
- krill
- imidazole
- breast
- Prior art date
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Landscapes
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Description
1、イミダペプチド(CBEX-Dr)配合飲料の健常者における抗疲労効果 薬理と治療 / 36
(3): 199-212, 2008 田中雅彰, 鴫原良仁, 藤井比佐子, 平山佳伸, 渡辺恭良 2、イミダゾールジペプチド配合飲料の日常的な作業の中で疲労を自覚している健常者に対する継続摂取による有用性 / 第1報 清水惠一郎, 福田正博, 山本晴章
3、健常者を対象としたイミダペプチド(CBEX-Dr)配合飲料の過剰摂取における安全性薬理と治療 / 36(3): 225-235, 2008青柳さやか, 杉野友啓, 梶本佳孝, 西谷真人。
実験材料及び方法
試薬
L-アンセリン(シグマ)
L-カルノシン(シグマ)
メタノール(ナカライテスク)
エタノール(ナカライテスク)
アセトニトリル(ナカイライテスク)
標準アミノ酸混合液AN2型(和光純薬製)
標準アミノ酸混合液B型(和光純薬製)
L-アスパラギン酸標準液(和光純薬製 アミノ酸自動分析用)
L(+)-グルタミン(試薬特級)
L-トリプトファン(試薬特級)
リン酸二水素アンモニウム(試薬特級)
ペンタンスルホン酸ナトリウム(イオンペアクロマトグラフ用)
アセトアニトリル(HPLC用)
塩酸(ナカライテスク)
鶏肉試料
大和シャモ(地鳥)((有)大和軍鶏提供)、鶏胸肉及びササミ、
脱脂乾燥オキアミ試料(水分1.1%)
実験方法
サンプル2.5g/25mldH2O(×10vol)をそれぞれホモジナイズ15秒3回ずつ行った。
それを遠心(10,000 rpm4℃20分)したものをフィルター(5c110mm)にとおした。フィルター後のサンプル300μlにメタノール900μlを加えたものを15分冷却(4℃)した。さらに、それを遠心(12,000g 4℃ 3分)したものから上清を回収した上清60μlに0.02NHCl 60μl加えたものをサンプルとしアミノ酸解析を行った。
分析方法
D-7000HPLC生体アミノ酸分析法
1、全自動アミノ酸分析計による分析方法
アミノ酸標準溶液の調製は、標準アミノ酸混合液AN2型、標準アミノ酸混合液B型(和光純薬製)、L-アスパラギン標準液(1.25μmol/ml)、2.5mmol/lグルタミン溶液、2.5mmol/lトリプトファン溶液それぞれを各1ml秤量し、アミノ酸自動分析計のサンプル希釈液で希釈し、全量を25mlにした。分析用試料を専用のバイアル瓶に1mlとり、全自動アミノ酸分析計を用いて生体アミノ酸分析モードで分析した。(注入量は50μl)
2、定性および定量
1 分離されたアンセリン・カルノシンの定性は保持時間により行った。
2 定量は標準溶液を用いた、内標を用いない絶対検量線法により行った。
3、HPLCによる分析方法
移動相は200mMリン酸二水素アンモニウム、100mMペンタンスルホン酸ナトリウム、4%(v/v)アセトニトリル、pH2.0(塩酸で調整)で調製した。分析条件は検出器の波長を220nm、恒温槽を30℃、流量を毎分0.8ml、注入量を20μlとした。
4、定性および定量
1 分離されたアンセリン・カルノシンの同定は保持時間により行った。
2 定量は標準試料を用いた、内標を用いない絶対検量線法によりおこなった。
試料および飼料
試料…大和シャモ(地鳥)・雄雌の胸肉及びササミ
飼料:コントロール飼料:マル中印大うす用配合飼料(中部飼料株式会社)のみ
飼料A:マル中印大うす用配合飼料(中部飼料株式会社)100重量部+乾燥オキアミ脱脂粉末5重量部
飼料B:マル中印大うす用配合飼料(中部飼料株式会社)100重量部+乾燥オキアミ脱脂粉末10重量部
飼料C:マル中印大うす用配合飼料(中部飼料株式会社)100重量部+乾燥オキアミ脱脂粉末1重量部
初めの60日間は全てコントロールの餌で飼育し、後半の60日間は後半の60日間は飼料A〜C及びコントロールの4種類に分け飼育した。それぞれの成分は以下の表2乃至表4の通りであった。
Claims (2)
- 鶏用飼料にオキアミ脱脂粉末又は/及びオキアミ抽出物を5〜10重量%混合することを特徴とする鶏の胸肉及びササミにおける、イミダゾールペプチド を増強するための鶏用飼料。
- 請求項1に記載の飼料を鶏に給飼することを特徴とする鶏の飼育方法。
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| JP2012248248A JP6120529B2 (ja) | 2012-11-12 | 2012-11-12 | 鶏用飼料及び鶏の飼育方法 |
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| JP2014093987A JP2014093987A (ja) | 2014-05-22 |
| JP6120529B2 true JP6120529B2 (ja) | 2017-04-26 |
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Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07107920A (ja) * | 1993-10-12 | 1995-04-25 | Nichiwa Sangyo Kk | 鶏用飼料 |
| JP5041228B2 (ja) * | 2007-10-19 | 2012-10-03 | 日環科学株式会社 | 消化力増強飼料、臭気軽減飼料、整腸剤型飼料、カロリー吸収率調整型飼料、肉質改善飼料、免疫力増強飼料、ならびに受胎率改善飼料、飼料型水質浄化剤、多機能性食品 |
| NO333013B1 (no) * | 2009-07-06 | 2013-02-18 | Smartfish As | Sammensetning omfattende bioaktive aminosyrer eller derivater derav og marin olje i en stabil olje-i-vannemulsjon, og fremgangsmate for fremstilling av nevnte sammensetning. |
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