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JP5303981B2 - Ｄｎａメチル化測定方法 - Google Patents

Ｄｎａメチル化測定方法 Download PDF

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Publication number
JP5303981B2
JP5303981B2 JP2008077968A JP2008077968A JP5303981B2 JP 5303981 B2 JP5303981 B2 JP 5303981B2 JP 2008077968 A JP2008077968 A JP 2008077968A JP 2008077968 A JP2008077968 A JP 2008077968A JP 5303981 B2 JP5303981 B2 JP 5303981B2
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JP
Japan
Prior art keywords
dna
solution
seq
methylated
base sequence
Prior art date
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Expired - Fee Related
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JP2008077968A
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English (en)
Japanese (ja)
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JP2008263961A (ja
Inventor
祥隆 冨ヶ原
日出夫 佐藤
弘和 樽井
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Sumitomo Chemical Co Ltd
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Sumitomo Chemical Co Ltd
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Priority to JP2008077968A priority Critical patent/JP5303981B2/ja
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Publication of JP5303981B2 publication Critical patent/JP5303981B2/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2008077968A 2007-03-26 2008-03-25 Ｄｎａメチル化測定方法 Expired - Fee Related JP5303981B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2008077968A JP5303981B2 (ja) 2007-03-26 2008-03-25 Ｄｎａメチル化測定方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2007078659 2007-03-26
JP2007078659 2007-03-26
JP2008077968A JP5303981B2 (ja) 2007-03-26 2008-03-25 Ｄｎａメチル化測定方法

Publications (2)

Publication Number Publication Date
JP2008263961A JP2008263961A (ja) 2008-11-06
JP5303981B2 true JP5303981B2 (ja) 2013-10-02

Family

ID=39831012

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2008077968A Expired - Fee Related JP5303981B2 (ja) 2007-03-26 2008-03-25 Ｄｎａメチル化測定方法

Country Status (2)

Country Link
JP (1) JP5303981B2 (fr)
WO (1) WO2008123537A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5266828B2 (ja) * 2008-03-25 2013-08-21 住友化学株式会社 メチル化されたｄｎａの含量を測定する方法
JP5832760B2 (ja) * 2011-02-28 2015-12-16 シスメックス株式会社 試料中のメチル化ｄｎａを検出する方法
CN109234388B (zh) * 2017-07-04 2021-09-14 深圳华大生命科学研究院 用于dna高甲基化区域富集的试剂、富集方法及应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5774805B2 (ja) * 2004-11-29 2015-09-09 セクエノム，インコーポレイティド メチル化ｄｎａを検出する方法、及びキット
JP4924014B2 (ja) * 2006-09-27 2012-04-25 住友化学株式会社 Ｄｎａメチル化測定方法

Also Published As

Publication number Publication date
JP2008263961A (ja) 2008-11-06
WO2008123537A1 (fr) 2008-10-16

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