JP3513873B2 - External preparation for skin - Google Patents

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION The present invention relates to a skin external preparation containing a combination of a specific medicinal ingredients and acerola extract, more particularly, has excellent anti-skin aging and skin roughness improvement effect,
The present invention relates to a skin external preparation that can give skin a luster and gloss.

[0002]

2. Description of the Related Art Conventionally, an external skin preparation such as an emulsion, a cream, a lotion, a pack, a dispersion, a detergent, an ointment, a cream or a liquid for external use has an object to impart a predetermined medicinal effect to them. As such, a medicinal component such as a cell activator or a moisturizer is added. For example, ginseng extract, Sikon extract for the purpose of improving rough skin, preventing skin aging, activating cells, treating wounds after cuts and shaving, and treating wounds such as cracks, cracks, sores, hemorrhoids, burns, etc. , A placenta extract, bovine blood deproteinization, a fermentative metabolite, and other medicinal components.

[0003]

[SUMMARY OF THE INVENTION However, in the external skin preparation containing a combination of cell activator, or not enough, the effect of cell-activating agent, or it is blended an amount sufficient to obtain the efficacy less stable However, there was a problem that the usability was lost. An object of the present invention is to provide an external preparation for skin that solves such problems.

[0004]

[Means for Solving the Problems] The present inventors
As a result of intensive studies to improve the effect of the medicinal component of the agent, by combining the acerola extract and the cell activating agent, it was found that the action of the intrinsic medicinal ingredient of the cell activating agent is sufficiently exerted, The present invention has been completed. That is, the present invention is a skin external preparation containing (A) an acerola extract and (B) a placenta extract which is a cell activating agent.

[0005]

BEST MODE FOR CARRYING OUT THE INVENTION The acerola extract, which is the component (A) of the external preparation for skin of the present invention, is a Malpighia genus.
The fruit tree of Acerola (scientific name Malpighia emar
ginata DC; The scientific name of acerola is differently written in papers and the like depending on the period, region, and researcher. Traditionally Malpig
hia punicifolia L. and Malpighia glabra L. are confused and used as the scientific name of acerola, but nowadays
Edited by Steven Nagy and others in 1980, "TROPI
Malpi described in "CAL AND SUBTROPICAL FRUITS"
ghia emarginata DC is the most suitable. Extraction is carried out from the fruit of 1) using an extraction solvent. The extraction treatment is not particularly limited, but extraction is performed at a low temperature or room temperature or under heating with a suitable solvent.

Examples of the above-mentioned extraction solvent include water; lower monohydric alcohols such as methyl alcohol and ethyl alcohol; liquid polyhydric alcohols such as glycerin, propylene glycol, and 1,3-butylene glycol, and one or more species thereof. Can be used. As an example of a preferred extraction method, purified water is used for extraction at a low temperature, followed by filtration, and 1,3-butylene glycol is further mixed to a concentration of 20 to 70% (W / W). , And a method of performing filtration again.

The compounding amount (content) of the acerola extract in the external preparation for skin of the present invention is preferably 0.00005 to 5% by weight as dry solid content (hereinafter, "% by weight" is simply represented by "%"). And more preferably 0.0005 to 1%. The content of this acerola extract is 0.00
If it is less than 005%, a sufficient effect may not be obtained, and if it exceeds 5%, no further increase in effect is observed.

The component (B) of the external preparation for skin of the present invention is
Placental extract. Placenta extracts from pigs, cows, etc.
Be done. One or two or more types may be appropriately selected and blended.
it can. This has a cell activating effect. In the composition of the present invention
The amount (content) of the placenta extract is preferably 0.
00001-10%, more preferably 0.0001-5
%. When using the extract as it is, it should be as dry solids.
Convert. If the amount of placenta extract is within this range,
When combined with acerola extract,
Acerola without affecting cerola extract
The extract acts on the placenta extract, and the skin external preparation is stable over time.
It has good activity, exerts an excellent cell activation effect, and is excellent
Have a feeling of use.

The external preparation for skin of the present invention is prepared by blending the essential components (A) and (B) with various types of bases known as ordinary external preparations for skin according to a conventional method. You can The form of the external preparation for skin is not particularly limited, and examples thereof include emulsions, creams, lotions, packs, skin care cosmetics such as cleaning agents, lipsticks, makeup cosmetics such as foundations, scalp cosmetics, and ointments. Dispersion,
It can be used as a medicine such as a cream agent or a liquid agent for external use, and its dosage form is not particularly limited, and solid, paste, mousse, gel, powder, solution, solubilization,
It can be emulsified, powder dispersed, or multi-layered.

In addition to the above-mentioned components (A) and (B), the external preparation for skin of the present invention usually contains cosmetics, quasi-drugs, external medicines, etc. within a range not impairing the effects of the present invention. Ingredients used in the formulation, namely water, oils, surfactants, metal soaps, gelling agents, powders, alcohols, water-soluble polymers, film-forming agents, resins, UV protection agents, inclusion compounds, antibacterial agents Agents, fragrances, deodorants, salts, pH regulators, cooling agents, animal / microorganism-derived extracts, plant extracts, blood circulation promoters, astringents, antiseborrheic agents, active oxygen eliminators, whitening agents, anti-inflammatory One or more kinds of agents, keratolytic agents, enzymes, hormones, vitamins and the like can be added appropriately.

[0019]

EXAMPLES Next Reference Example, and Test Examples and Examples that explains the invention in more detail.

Reference Example 1. Production of acerola extract Acerola (scientific name: Malpighia emarginata DC)
Purified water was added to the fruit of the above, extraction was performed at low temperature for 1 day, and then filtration was performed to obtain an acerola extract (dry solid content 1.7).
%).

Reference Example 2. Preparation of placenta extract 1 kg of placenta of a cow 3-4 months pregnant is frozen and thawed, impurities are washed and removed, 5 liters of purified water is added, extraction is performed under reduced pressure and low temperature for 3 days, and placenta extract is filtered. Was obtained (dry solid content 3.5%). This extract is a cell activator.

Reference Example 3. Production of Panax ginseng root 250 g Panax ginseng root was shredded, 1 liter of absolute ethanol was added, and the mixture was stirred and extracted with a blender for one day to obtain a carrot extract (dry solid content 1.1%). This
The extract is a cell activator.

Reference Example 4 Preparation of Lactic Acid Bacteria Extract 1 liter of an aqueous milk protein solution is inoculated with 1% of Lactobacillus and cultured at 40 ° C. for 3 days. 80 fermented milk obtained
Instant sterilization at ℃, filtered to obtain a lactic acid bacterium extract (dry solid content 9.5%). This extract is a cell activator.

Test Example 1. Fibroblast proliferation test An appropriate amount of medium is placed in a petri dish, human neonatal fibroblasts are seeded, and the mixture is allowed to stand at 37 ° C in a carbon dioxide concentration of 5%. The next day, freeze-dried cell activating agents shown in Table 1 were used at a concentration of 100 μg / ml and the acerola extract was 100 mg / m 2.
It is dissolved in PBS (phosphate buffer solution) so that the amount becomes 1, and 1/10 amount of the medium is added to a petri dish and mixed. As a control
Add PBS. The cell number (A) and the control cell number (B) for each sample were measured, and the cell growth rate was calculated by the following formula. The results are shown in Table 1. Cell growth rate (%) = (A / B) × 100 where A: number of cells when sample is added B: number of cells when sample is not added

[0029]

[Table 1]

As is clear from the results in Table 1, by combining the acerola extract with the placenta extract and / or the ginseng extract which are cell activating agents, a very high cell growth promoting effect was obtained. To be

Reference Example 1 Preparation Example of Emulsion An emulsion having the composition shown in Table 2 was prepared, and its skin aging prevention effect was examined. The results are also shown in Table 2 . AT in the table
P, 17β-estradiol is a cell activator.

[0037]

[Table 2]

(Production Method) A. A part of the component (14) and (6), (11) and (13) are mixed by heating and kept at 70 ° C. B. The components (1) to (5) are mixed by heating and kept at 70 ° C. C. The above B is added to the above A and mixed, and the component (10) is added to uniformly emulsify, and the mixture is cooled to 30 ° C. The component (12) is added and mixed uniformly. D. The components (7) to (9) are mixed with the rest of the component (14), added to C and uniformly mixed to obtain an emulsion.

(Test Method) For each test emulsion, 15 women aged 26 to 50 were used as a panel. After washing the face twice daily in the morning and at night for 12 weeks, an appropriate amount of the test emulsion was applied to the face. The skin aging prevention effect by application was evaluated according to the following criteria.

Skin Aging Preventive Effect: [Evaluation] [Contents] Effectiveness The skin's firmness and gloss were improved. Slightly effective Skin tone and gloss were slightly improved. Ineffective No change from before use. The results obtained by the above evaluation method are also shown in Table 3.
As is clear from the results of Table 2 , the emulsion containing the acerola extract and the cell activator according to the present invention, when applied to the skin, improves the skin's elasticity and gloss and prevents skin aging. I was able to.

Reference Example 2 Preparation Example (Prescription) of Cosmetic Lotion (%) (1) Glycerin 5.0 (2) 1,3-Butylene Glycol 6.5 (3) Polyoxyethylene (20 EO) Sorbitan 1.2 Monolauric acid ester (4) Ethyl alcohol 8.0 (5) Acerola extract * 1 1.0 (6) Quince extract * 2 1.0 (7) Barley extract * 3 1.0 (8 ) Preservative Suitable amount (9) Perfume Suitable amount (10) Alkaline simple hot spring water * 4 50.0 (11) Purified water Remaining amount * 1 Manufactured in Reference Example 1 * 2 Koei Kogyo Co., Ltd. * 3 Technoble Co., Ltd. : Cell activating agent * 4 manufactured by Maruzen Pharmaceutical Co., Ltd. (manufacturing method) A. Components (3), (4), (8) and (9) are mixed and dissolved. B. Components (1), (2), (5) to (7), (10) and (11) are mixed and dissolved. C. A and B were mixed and made uniform to obtain a lotion.

Reference Example 2 was a lotion which was excellent in stability over time, improved the firmness and gloss of the skin, and had an excellent moisturizing effect and rough skin improving effect.

Reference Example 3 Production Example of Lipstick (Formulation) (%) (1) Acerola Extract * 1 0.02 (2) γ-Linolenic Acid * 2 0.02 (3) Lactic Acid Bacteria Extract * 30 0.001 (4) Red No. 202 0.2 (5) Candelilla wax 9.0 (6) Solid paraffin 8.0 (7) Beeswax 5.0 (8) Carnauba wax 5.0 (9) Lanolin 11.0 ( 10) Isopropyl myristate 10.0 (11) Refined castor oil Remaining amount (12) Preservative Suitable amount (13) Perfume Suitable amount * 1 Manufactured in Reference Example 1 * 2 Idemitsu Petrochemical * 3 Manufactured in Reference Example 4 What I did

(Production Method) A. After the components (5) to (12) are dissolved by heating, they are kneaded with three rollers. B. After adding components (1) to (3) and (13) to A, repeat 3 again.
Knead with a main roller. C. B was cooled and molded to obtain lipstick. Reference Example 3 was a lipstick which prevented roughening of the lips and gave good gloss and firmness.

[0051]

The external preparation for skin of the present invention exhibits by blending and placenta extract acerola extract and cell activator, placenta
It is possible to fully exert the inherent efficacy of the extract . The external preparation for skin in which a placenta extract is used as a medicinal component is stable and has an excellent anti-aging effect on skin, and therefore maintains the skin's elasticity and gloss and exerts a high inhibitory effect on wrinkle formation . Thus, the external preparation for skin of the present invention was used as a cell activating agent by blending with the acerola extract .
Since it is possible to improve the inherent medicinal effect of the placenta extract, it is extremely useful in beauty and medicine.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI A61K 35/78 A61K 35/78 C A61P 17/00 A61P 17/00 (72) Inventor Shizuka Uehara 48-18 Sakaemachi, Kita-ku, Tokyo No. 48 Kose Research Headquarters (72) Inventor Ichiro Sasaki 48-18 Sakaemachi, Kita-ku, Tokyo Kose Research Headquarters (72) Inventor Kenichi Nagamine 2-5-1 Misumicho, Higashimurayama-shi, Tokyo -203 (72) Inventor Takashi Kido 1-21-25-103 Namiki, Kawaguchi City, Saitama Prefecture (56) Reference JP-A-2-200610 (JP, A) JP-A-10-109927 (JP, A) JP 10-95704 (JP, A) JP-A-7-277939 (JP, A) JP-A-10-114670 (JP, A) JP-A-9-291011 (JP, A) JP-A-9-183718 (JP , A) (58) Fields surveyed (Int.Cl. ⁷ , DB name) A61K ^7/ 00- 7/50

Claims (2)

(57) [Claims] 1. A skin external preparation comprising the following components (A) and (B): (A) acerola extract (B) placenta extract . 2. The external skin application according to claim 1, wherein the blended amount of the acerola extract is 0.00005 to 5% by weight as a dry solid content, and the blended amount of the placenta extract is 0.00001 to 10% by weight. Agent.