JP2819033B2 - An external preparation for skin or hair or a cosmetic containing a growth inhibitor of Propionibacterium acnes - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION <A> Object of the Invention The present invention relates to a novel plant-derived extract (extract), Proponibacterium acnes (Propionibacteriu).
acnes), or an external preparation for skin or hair or a cosmetic containing the growth inhibitor.

"Industrial application field" The growth inhibitor of Propionibacterium acnes according to the present invention can be used in the form of external use, pharmaceuticals, quasi-drugs,
It is used in the formulation of cosmetics, and is useful for preventing and preventing purulent diseases caused by so-called P. acnes of acne bacteria. It can also be used for internal use.

"Prior art" Acne is a skin disease that mainly develops during puberty, and its name is acne vulgaris. Clinically, it is a type of chronic inflammatory disease that occurs in the pores mainly in the pilosebaceous system. It is considered one.
Factors causing acne include sebaceous follicles, endocrine factors, keratinization of hair follicles, bacteria in hair follicle tubes, and genetic factors.

It is said that people with acne have a higher amount of skin surface lipids than normal people, and that the amount corresponds to the severity of acne.

Regarding the onset of acne, according to studies from a biochemical angle, triglyceride in sebum increased lipase production due to the invasion of propioniobacterium acnes into the hair follicle. By
It is known that the production of glycerol and free fatty acid is enhanced, and the excess free fatty acid produced at this time has a particularly strong comedone-forming action and an inflammation-inducing action.

Originally, Propionibacterium acnes is lipid-dependent and lipophilic, and as sebum increases and accumulates in the follicles, propionibacterium acnes also gradually increases. Have been.

In addition, neutrophil chemotactic ability and complement activating factor by Propionibacterium acnes, and destruction of hair follicle epithelium by bacterium's products such as protease, lipase, and hyaluronidase, and induction of inflammation Are considered as new hypotheses, and in addition to the above factors, internal and external factors such as genetic factors, age, dietary factors, stress, irregular menstruation, mechanical irritation, cosmetics, etc. are mutually complicated. It is thought that it develops in connection with, but in any case,
The mechanism of acne development was thought to be extremely important in preventing acne infestation (invasion and growth) of propionibacterium acnes in the treatment of acne.

Therefore, creams and ointments containing an antibacterial action (antibiotic), a sebum secretion inhibitor, a keratolytic agent, and the like have been generally used as an external preparation for acne treatment.

However, existing treatments for acne have various drawbacks. For example, female hormones, which are one of the sebum secretion inhibitors, have side effects and are not preferable, and colloidal sulfur, which is a representative of the keratolytic agent, is used. In many cases, continuous administration of sulfur compounds will cause skin irritation and skin swelling, and topical creams and ointments such as antibiotics and their use are also effective. The use of the drug is limited under the control of the company, and a prescription from a doctor is required to sell it for general over-the-counter use.

"Problems to be Solved by the Invention" In view of the above circumstances, the present inventors have sought to obtain a propionibacterium acnes growth inhibitor from various plants.

<B> Structure of the Invention The present invention provides a professional extract comprising a plant extract selected from one or more selected from the group consisting of gerep, soho tree, yaw nut, and logwood as an active ingredient. An external preparation for skin or hair or a cosmetic containing a growth inhibitor of Pionibacterium acnes.

"Means to solve the problem" Investigating the effects of plant-derived components (Kampo preparations) frequently used on acne on Propionibacterium acnes, for example, a report by Konishi et al .: Kampo medicine
10. ( No. 7), 14 (1986), and it is known that Oren and Huangbashi have a considerably strong inhibitory action. It is considered that the main component in both plants is an alkaloid (berberine) which is an isoquinoline-based quaternary group.

In addition, it has been reported that thorn, licorice, yellow gon, bitter ginseng, serial forsythia, and gardenia show a weak inhibitory effect, and from the viewpoint of effectiveness, it is equivalent to or higher than the above-mentioned yellow orchid, yellow oak Was required.

Thus, the present inventors obtained an extract based on many plants, and then tested the inhibitory effect on Propionibacterium acnes. As a result, Akane, Asenyaku,
Aloe, Epimedium, Ginkgo, Ginkgo biloba, Fenka, Fruit, Ephedra, Hypericum grass, Huangkashi, Yellow gon, Licorice, Summer hay, Walnut leaf, Bitter ginseng, Gerep, Cinnamon bark, Tea, Five times child, Coffee beans, Saiko, Pomegranate Skin, purple root, persimmon, peony, ginger, shouma, heavy medicine, sokataki, mulberry white skin, sojutsu, assembly, bamboo ginseng, choji, chen skin, peach skin, peach meat, plum meat, juniper, light load, betel palm, safflower, Water or hot water extraction or hydrophilic (organic) solvent extraction from peony skin, mangrove, myrobaran, yaw berries, Yangmei skin, ginger, green tea, forsythia, log wood, sage, and concentrated extract Has an inhibitory effect on Propionibacterium acnes. Based on this finding, the present inventors searched for a substance which exhibited a strong inhibitory action, had a good stability of the extraction solution, and could be easily incorporated into cosmetics, and searched.

"Confirmation of growth inhibitory effect (effect) on Propionibacterium acnes" (a) Test method Propionibacterium acnes (Propionibacterium ac) isolated and identified from the acne patient's facial rash
nes), using GAM agar medium, Gas Pak (Mic manufactured by BBL)
An anaerobic culture was performed at 37 ° C. for 48 hours using Robioloy Systems). On a GAM agar medium in a Petri dish, 0.1 ml of the pre-cultured bacterial solution was uniformly applied on the surface of the plate medium, and a TOYO paper disk (diameter: 10 mm) was raised thereon. Plant extract (50 μL) was applied thereto, and cultured at 37 ° C. for 48 hours, and the diameter of the formed inhibition circle was measured.

(B) Performance Results Table 1 shows the results of the growth inhibitory effect on Propionibacterium acnes. In the final evaluation, the present inventors deemed that those having a block diameter of 10 mm or less had no growth inhibitory action.

As a result, many plant extracts were sieved.
In other words, those that had no effect based on their blocking diameter were as follows: Fenka, Yomi, Totogi grass, Licorice, Summer hay, Walnut leaf, Cinnamon bark, Black tea, Five times child, Coffee beans, Persimmon, Shakuyaku, Masu Hemp, bamboo ginseng, Chen skin, peaches, plum meat, juniper, betel nut, peony skin, mangrove, mirobaran, and ginger.

On the other hand, as shown in Table 1, those having the same level of growth inhibitory activity as Huangkashia are, as shown in Table 1, four types of extracts, namely, gerep, sokataki, yawatsu, and logwood. Among them, it was confirmed that there was a strong inhibitory effect (effect), with the stopping circle diameter of Sokata tree, the fruit of the arrow wheel and the logwood being 20 to 22 mm. In addition, all of these four extracts have good solubility and stability in water or ethanol.

"Extraction-derived plants" Plants used in the present invention are as follows.

Gerep is a dried bark or heartwood of Moraceae gerep, also known as fustic.

Suhogi is a dried heartwood of leguminous (Leguminosae) suhou.

The fruit of the arrow wheel uses the solid fruit of the birch family (Betulaceae), i.e., the fruit of the herbal medicine name "Yahari".

Logwood is dried heartwood from leguminous (Leguminosae) logwood.

"Manufacturing Method" (Example 1) Gerep is extracted at about 10 to 40 ° C. using water or hot water and a hydrophilic organic solvent (for example, ethanol, methanol, or the like, or appropriately diluted with water). And, as it is or as appropriate, concentration is performed to obtain an extract.
In addition, it is also possible to use a paste-like material or a powder.

Although the formulation (formulation example) will be described later, it can be arbitrarily blended and used in the formulation of known cosmetics for hair and skin. Further, with respect to the compounding amount, both powdered and extract (residue after distilling off the extraction solvent) are included in the total composition at about 0.5%.
It is desirable that the content be 01% (% by weight) or more. The upper limit of the amount is not particularly limited, but may be about 0.1 to 0.5% for the purpose of preventing or treating acne.

For acne that is red and swollen on the verge of suppuration, a tincture containing an extract extracted locally or a lotion, cream, etc. attached to absorbent cotton and stopped with an adhesive bandage will cause the swelling to be released quickly and pain In many cases, swelling and pain are often healed quickly, especially in a preparation in a hydrophilic cream type emulsifying system.

(Example 2) In the same manner as in Example 1, an extract of sokataki, fruit of yaw, logwood, and each extracted extract are obtained. Incidentally, the manufacturing method is not limited to the embodiment, and other known methods may be used.

"Formulation example" (Formulation example 1) Lotion (%) Extract of citrus fruit 0.3 Glycerol 3.0 1,3-butylene glycol 3.0 Sodium citrate 0.1 Ethanol 10.0 Polyoxyethylene oleyl alcohol 0.5 Paraben 0.1 Purified water Residue [Production method] The above components are dissolved at room temperature, and similarly, the components mixed and dissolved at room temperature are added with stirring to obtain a lotion.

(Formulation Example 2) Cleansing foam (%) Gerep extract 0.3 Soho tree extract 0.2 Glycerin 20.0 Palmitic acid 10.0 Stearic acid 10.0 Myristic acid 10.0 Lauric acid 5.0 Oleyl alcohol 1.0 Potassium hydroxide 6.0 Purified water Residue [Production method] And heat, add to this,
Immediately heat to 70 ° C. At the same time, it was previously heated and dissolved, and slowly added with stirring, and then left at 70 ° C. to terminate the saponification reaction.
Get a cleansing foam.

(Formulation Example 3) Cream for acne (%) Soho tree extract 0.3 1,3-butylene glycol 5.0 Photosensitizer 201 0.002 Beeswax 2.0 Cetanol 4.0 Reduced lanolin 11.0 Squalane 40.0 Paraben 0.2 Polyoxyethylene monosorbitan monolaurate 2.0 Purification Water residue [Production method] Add the above ingredients, heat and maintain at 70 ° C (aqueous layer). Separately, other components were mixed and dissolved by heating to 70 ° C. (oil layer portion). This (oil layer portion) is added to the (water layer portion), preliminary emulsification is performed, and the mixture is uniformly emulsified with a homomixer.
Obtain O / W type cream.

(Formulation Example 4) Packing agent (%) Yarrow extract 0.2 Gerep extract 0.2 Vinyl acetate resin emulsion 13.0 Polyvinyl alcohol 10.0 Olive oil 3.0 Sorbit 5.0 Titanium oxide 16.0 Ethanol 10.0 Paraben 0.05 Purified water Residue [Production method] Mix the above ingredients And add it and
Further, the mixture moistened with a part of the mixture is added and dissolved by heating at 70 ° C. Next, the remaining ingredients are added and mixed, and finally, after adding and cooling, a pack is obtained.

(Prescription example 5) Foundation (%) Logwood extract extract 0.4 Titanium oxide 13.0 Colloidal kaolin 25.0 Talc 45.0 Bengala 1.0 Yellow iron oxide 2.5 Black iron oxide 0.1 Liquid paraffin 7.5 Sorbitan sequisoleate 3.4 Glycerin 2.0 Paraben 0.1 [Preparation method] Using a pulverizer, mix and pulverize to an average particle size of 1 to 5 μm.
Was added and mixed. Separately, the mixture is mixed, and the resulting mixture is added to the above mixture, followed by further uniform mixing. This is treated with a crusher, passed through a sieve, and subjected to compression molding with uniform particle size to obtain a cake-type foundation.

(Formulation Example 6) Hair tonic (%) Logwood extract extract 0.5 Assembly extract 1.0 Salicylic acid 0.5 Glycerin 1.0 Ethanol 40.0 Purified water Residue [Production method] Add the above ingredients, mix and dissolve at room temperature, and mix at room temperature as well. Stirring is added to the previously prepared solution to obtain a hair tonic.

(Formulation Example 7) Shampoo (%) Extract of corn seeds 0.5 Extract of logwood 0.5 Triethanolamine lauryl sulfate 15.0 Diethanolamide laurate 1.0 Methyl paraben 0.1 Propyl paraben 0.1 Purified water Residue (Formulation example 8) Rinse (%) Gerep extraction Extract 0.5 Logwood extract 0.3 Stearyltrimethylammonium chloride 2.0 Propylene glycol 6.0 Purified water Residue <c> Effect of the present invention The present invention is based on the fact that a plant extract specified as a growth inhibitor against Propionibacterium acnes is formulated. The present invention relates to an external preparation or cosmetic for skin or hair. According to the present invention, the effect is that it has little irritation to the skin and is effective in preventing and treating acne. Seborrheic eczema, dandruff and hair loss on the head can be prevented. Propionibacterium
As shown in Table 1, the growth inhibitor for acnes acts as an antioxidant for products containing various lipids because it has a strong inhibitory effect in a small amount, or as a stabilizer for fats and oils. Useful. In the preparation of a skin external preparation or cosmetic containing the plant extract according to the present invention, during the formulation, if necessary, a surfactant, a humectant, a lower alcohol, a thickener, a fragrance, an oxidant, etc. Inhibitors, chelating agents,
Known and commonly used components such as dyes and preservatives can be freely combined and blended.

────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ identification code FI A61K 35/78 A61K 35/78 J (58) Investigated field (Int.Cl. ⁶ , DB name) A61K 7/00-7 / 50 A61K 35/78

Claims (1)

(57) [Claims] 1. A propionibacterium acnes comprising, as an active ingredient, a plant extract selected from one or more of the group consisting of gerep, sohoki, yaw nut, and logwood. An external preparation for skin or hair or a cosmetic containing a growth inhibitor.