JP2018177751A - Oil-in-water emulsion composition, external preparation for skin and method for preparing external preparation for skin - Google Patents

Abstract

To provide an oil-in-water emulsion composition excellent in storage stability capable of suppressing fading of astaxanthin even when stored for a long period of time and maintaining good appearance, and external skin application including the oil-in-water emulsion composition An agent and a method for producing an external preparation for skin are provided.
An oil-in-water emulsion composition containing components A to F and its application.
A: Astaxanthin B: Tocopheryl nicotinate C: Sucrose saturated fatty acid ester D: At least one emulsifier selected from emulsifiers having unsaturated fatty acid residues E: Glycerin F: Water [Selection chart] None

Description

  The present disclosure relates to an oil-in-water emulsion composition, a skin external preparation, and a method of producing a skin external preparation.

Conventionally, addition of an oil component to aqueous beverages and aqueous foods and aqueous cosmetics has been performed. However, since the oily component is insoluble or poorly soluble in water, it has been general to disperse the oily component in an aqueous medium using a means such as emulsification to form an oil-in-water emulsion composition. In an oil-in-water emulsion composition, light is scattered depending on the particle size of the dispersed particles containing an oil component, so when the particle size is sufficiently small, it exhibits a transparent appearance by visual observation, but the particle size is large In such a case, transparency by visual observation may be reduced, and turbidity may occur, which may cause an undesirable appearance.
The emulsified composition is generally in a metastable state, and even if it is transparent by visual observation immediately after preparation, aggregation of the emulsified particles may progress during storage, and when aggregation progresses, turbidity due to increase in emulsified particle size occurs And there is concern that transparency may decline. In particular, the smaller the emulsified particles, the more difficult the stabilization.
Also, astaxanthin, which is known as a functional material, has a red color and has a function as a colorant derived from a natural product. Therefore, the emulsion composition containing astaxanthin exhibits a clear red color, and can give a preferable appearance to various preparations including the emulsion composition and the emulsion composition.

In order to maintain a transparent appearance, various studies have been made to stably hold dispersed particles having a small particle size in an emulsion composition.
For example, a carotenoid having a small emulsified particle and excellent storage stability, which contains tocopherol and lecithin in an oil phase and a water-soluble emulsifier selected from sucrose fatty acid ester, polyglycerin fatty acid ester, and sorbitan fatty acid ester Contained emulsion compositions have been proposed (see, for example, Patent Document 1).
In addition, it contains a specific amount of a fat-soluble component, sucrose fatty acid ester, polyglycerin fatty acid ester, phospholipid, polyol, and water, and is excellent in emulsion stability and transparency applicable to aqueous foods and beverages. An emulsion composition has been proposed (see, for example, Patent Document 2).
Furthermore, a thioctic acid-containing composition having excellent storage stability, which comprises a polyglycerin fatty acid ester which contains thioctic acid which is a poorly soluble component in water and which has an HLB (hydrophilic lipophile balance) of 9 or more. Has been proposed (see, for example, Patent Document 3).

Patent No. 5459939 gazette Patent No. 5675079 JP 2007-16000 A

  In the technology described in each of the above patent documents, the dispersed particles containing the oil component are small, and the storage stability is also studied for the temporal stability under the temperature condition of room temperature or higher. However, according to the study of the present inventors, in the emulsion compositions described in Patent Documents 1 to 3, when the preparation is prepared by adding a carotenoid such as astaxanthin to the emulsion composition, the preparation is transparent. It was found that when stored in a transparent container and stored over time for the purpose of taking advantage of the appearance, discoloration such as astaxanthin contained in the emulsion composition may occur, resulting in a change in the appearance.

An object of one embodiment of the present invention is to provide an oil-in-water emulsion composition excellent in storage stability in which the discoloration of astaxanthin is suppressed even when stored for a long period of time, and a good appearance can be maintained. It is.
An object of another embodiment of the present invention is to provide a preparation for external use on skin and a preparation for external use on skin having excellent storage stability capable of maintaining good appearance with astaxanthin even when stored for a long period of time. It is.

  Means for solving the above problems include the following embodiments.

The oil-in-water type emulsion composition containing <1> A component-F component.
A: Astaxanthin B: Tocopheryl nicotinate C: Sucrose saturated fatty acid ester D: At least one emulsifier selected from emulsifiers having unsaturated fatty acid residue E: Glycerin F: Water <2> Unsaturated fatty acid residue in D component The oil-in-water type emulsion composition as described in <1> which is an unsaturated fatty acid residue chosen from an oleic acid residue, a linoleic acid residue, and a linolenic acid residue.
The oil-in-water type emulsion composition as described in <1> or <2> which contains at least 2 types of <3> D components.

The oil-in-water type emulsion composition as described in any one of <1>-<3> whose <4> D component contains a nonionic emulsifier.
The oil-in-water type emulsion composition as described in <4> in which a <5> nonionic emulsifier contains polyglyceryl.
The oil-in-water type emulsion composition as described in <5> whose average polymerization degree of <6> polyglyceryl is 5-20.

The oil-in-water type emulsion composition as described in any one of <1>-<6> in which <7> D component contains an amphoteric emulsifier.
The oil-in-water type emulsion composition as described in <7> in which a <8> amphoteric emulsifier contains a lecithin.

The skin external preparation containing the oil-in-water type emulsion composition as described in any one of <9><1>-<8>.
<10> A step of preparing the oil-in-water emulsion composition according to any one of <1> to <8>, and 0.0001% by mass to 10% by mass of the oil-in-water emulsion composition, and at least one of them. And b) preparing a mixture containing the vehicle of

An object of one embodiment of the present invention is to provide an oil-in-water emulsion composition excellent in storage stability in which the discoloration of astaxanthin is suppressed even when stored for a long period of time, and a good appearance can be maintained. It is.
An object of another embodiment of the present invention is to provide a preparation for external use on skin and a preparation for external use on skin having excellent storage stability capable of maintaining good appearance with astaxanthin even when stored for a long period of time. It is.

  Hereinafter, an example of the embodiment of the oil-in-water emulsion composition to which the present invention is applied will be described. However, the present invention is not limited to the following embodiments at all, and can be implemented with appropriate modifications within the scope of the object of the present invention.

The numerical range shown using "-" in this specification means the range which includes the numerical value described before and after "-" as minimum value and the maximum value, respectively.
In the numerical ranges that are described stepwise in the present specification, the upper limit or the lower limit described in one numerical range may be replaced with the upper limit or the lower limit of the numerical range described in the other stepwise Good. In addition, in the numerical range described in the present specification, the upper limit value or the lower limit value of the numerical range may be replaced with the value shown in the example.
In the present specification, the amount of each component in the composition is the total amount of a plurality of substances present in the composition unless a plurality of substances corresponding to each component are present in the composition. Means
As used herein, the term "water phase" is used as the term for the "oil phase" regardless of the type of solvent.
In the present specification, the normal temperature means "25 ° C." unless otherwise specified.

<Oil-in-water type emulsified composition>
The oil-in-water emulsion composition of the present disclosure (hereinafter sometimes referred to as an emulsion composition) is an oil-in-water emulsion composition containing the components A to F.
A: astaxanthin B: tocopherol nicotinate C: sucrose saturated fatty acid ester D: at least one emulsifier selected from emulsifiers having unsaturated fatty acid residues E: glycerin F: water

The emulsion composition of the present disclosure suppresses discoloration of astaxanthin which is the component A even when stored for a long period of time by containing the components B to F, and when it is placed in a transparent container, it produces a red color derived from astaxanthin The transparent content of is visible and good appearance is maintained for a long time. Therefore, when the emulsion composition of the present disclosure is used as a skin external preparation as it is, it can be expected to impart a good appearance and a function of astaxanthin. Also, for example, even when the emulsion composition of the present disclosure is mixed with an aqueous medium or the like to form a skin external preparation, the skin external preparation is crimson and excellent in transparency, and a good appearance is maintained for a long time.
The emulsion composition of the present disclosure is excellent in light resistance even when stored for a long period of time, that is, the discoloration due to the photodegradation of astaxanthin is suppressed.
Furthermore, the emulsion composition is excellent in transparency, and excellent in storage stability capable of maintaining a good appearance.

In the oil-in-water emulsion composition of the present disclosure, oil phase particles are present dispersed in the water phase.
Sucrose saturated fatty acid ester (component C) is known to be useful as an emulsifier. Furthermore, the emulsion stability is further improved by using at least one emulsifier (component D) selected from emulsifiers having unsaturated fatty acid residues in combination with the component C.
Glycerin (E component) is a kind of polyhydric alcohol, which lowers the interfacial tension between the water phase component containing water (F component) and the oil phase component, which makes the interface easy to spread, fine and , Has a function of facilitating the formation of stable oil phase particles. Furthermore, the component E is useful for formulation because it has a moisturizing function, a viscosity adjusting function, and the like.
In the emulsion composition of the present disclosure, coexistence of astaxanthin (component A) and tocopherol nicotinate (component B) in oil phase particles is more than in the case of coexistence of tocopherol known as an oil-soluble antioxidant. It is considered that the affinity with the oil component including the A component is better, the color fading of the A component by light, that is, the decomposition of astaxanthin by light is suppressed, and the light resistance is improved.
Furthermore, since the D component has an unsaturated fatty acid residue, the interaction at the interface between the C component and the oil phase particle described above is less likely to be formed as compared with the emulsifier having a saturated fatty acid residue. Therefore, the generation of turbidity caused by the crystallization of the oil component contained in the oil phase particles is suppressed, and the transparent state is maintained. Furthermore, it is considered that a good appearance is maintained for a long period of time, in combination with the already described fading suppressing effect of the component A.
Note that the present disclosure is not at all limited to the above estimation mechanism.

  Hereinafter, each component contained in the emulsion composition of the present disclosure will be described.

[A ingredient: astaxanthin]
The emulsion composition of the present disclosure contains at least one of astaxanthin and its derivatives. The derivatives of astaxanthin in the present specification include esters of astaxanthin and the like.
In the present specification, unless otherwise specified, astaxanthin and its derivatives are generically referred to as “astaxanthin”.

As astaxanthin, in addition to those derived from natural products such as plants, algae, crustaceans and bacteria, any may be used as long as it can be obtained according to a conventional method.
Examples of astaxanthin which is a natural product include red yeast Phaffia, Haematococcus alga, marine bacteria (for example, Paracoccus), krill, Adonis (for example), and the like. Mention may also be made of extracts from cultures of natural products.
Astaxanthin may be one obtained by appropriately purifying one of these natural products separated or extracted, as necessary, or a synthetic product.
As astaxanthin, those extracted from Haematococcus algae (also referred to as Haematococcus alga extract) and pigments derived from krill (also referred to as an krill extract) are preferable from the viewpoint of quality or productivity.

Astaxanthin may be a commercially available product. As a commercial product of astaxanthin, Haematococcus alga extract and Krill extract can be used.
As a commercial item of the Haematococcus alga extract, For example, Fujifilm Co., Ltd. ASTOTS (registered trademark)-S, ASTOTS (registered trademark)-SS, ASTOTS (registered trademark)-5OS, ASTOTS (registered trademark)-5 O, ASTOTS (registered trademark)-5OS, ASTOTS (registered trademark)-10 O, etc. (as described above, trade names), Astarille (registered trademark) oil 50F, Astarille (registered trademark) oil 5F of Fuji Chemical Industry Co., Ltd. And Toyo Asuka Chemical Co., Ltd. BioAstin (registered trademark) SCE7, Asta Pure of Arga Technology Co., Ltd. (a product containing 10% by mass of astaxanthin, a product containing 20% by mass by weight), and the like.
As a commercial item of the krill extract, Astax ST (brand name) of Marine Daioh Co., Ltd., etc. are mentioned.

Astaxanthin can be used as an astaxanthin-containing oil contained in an oil component.
In the astaxanthin-containing oil containing astaxanthin and an oil component, the content of astaxanthin is preferably 0.001% by mass to 50% by mass, more preferably 0.01% by mass to 25% by mass, and more preferably 5% by mass to 25%. % By mass or less is more preferable, and 10% by mass or more and 25% by mass or less is particularly preferable.
Since a carotenoid such as astaxanthin is a component that is susceptible to oxidative degradation, the emulsion composition of the present disclosure can also store astaxanthin under refrigeration for the purpose of further suppressing oxidative degradation.

  The content as an astaxanthin-containing oil in the emulsion composition of the present disclosure can be determined from the viewpoint of the stability of the emulsion composition, and for example, 0.001% by mass or more and 30% by mass relative to the total mass of the emulsion composition. % Or less is preferable, 0.01 mass% or more and 20 mass% or less is more preferable, 0.1 mass% or more and 15 mass% or less is more preferable.

[B ingredient: tocopherol nicotinate]
The emulsion composition of the present disclosure contains tocopherol nicotinate.
Tocopheryl nicotinate is an oil-soluble ester derivative of vitamin E, and its antioxidant activity as vitamin E can be expected.
In the emulsion composition of the present disclosure, the component B is considered to be useful for preventing oxidation and suppressing discoloration of the component A.
As tocopherol nicotinate, it is preferable to use a raw material used in cosmetic applications and quasi-drug applications.
Furthermore, when the emulsified composition of the present disclosure contains tocopherol nicotinate, for example, when the emulsified composition is applied to a skin external preparation, improvement of lipid metabolism which is the function inherently possessed by tocopherol nicotinate, blood flow promotion, Functions such as cell membrane reinforcement are expected.

  0.01 mass% or more and 3.0 mass% or less are preferable with respect to the total mass of an emulsion composition, and the content rate of the tocopherol nicotinate with respect to the emulsion composition total mass is 0.05 mass% or more and 2.5 mass% or less Is more preferably 0.1% by mass or more and 2.0% by mass or less.

[C ingredient: sucrose saturated fatty acid ester]
The emulsion composition of the present disclosure contains sucrose saturated fatty acid ester.
The sucrose saturated fatty acid ester includes an ester of a saturated fatty acid having 12 to 18 carbon atoms, for example, a saturated fatty acid selected from lauric acid, myristic acid, palmitic acid, and stearic acid and sucrose.
More specifically, sucrose saturated fatty acid esters include sucrose monolaurate, sucrose dilaurate, sucrose trilaurate, sucrose tetralaurate sucrose, sucrose monopalmitate sucrose, dipalmitate sucrose, tripalmitate sucrose, tetrapalmitate sucrose Sucrose monomyristate, sucrose dimyristate, sucrose trimyristate, sucrose tetramyristate, sucrose monostearate, sucrose distearate, sucrose tristearate, sucrose tetrastearate and the like.
Among them, sucrose fatty acid esters having at least one HLB 3 or more selected from stearic acid, myristic acid, palmitic acid, and lauric acid as saturated fatty acids are preferable, and sucrose fatty acid esters having HLB 3 or more wherein fatty acids are stearic acid are particularly preferable. Preferably, sucrose stearic acid esters of HLB5 to HLB15 are more preferred.

  A commercial item can be used as sucrose saturated fatty acid ester. As commercially available products, sucrose saturated fatty acids having different degrees of esterification such as Ryoto (registered trademark) sugar ester series (Mitsubishi Chemical Foods Ltd.), DK ester (registered trademark) series (Daiichi Kogyo Seiyaku Co., Ltd.), etc. Commercial products having different HLBs depending on the composition ratio, such as monoesters and sucrose saturated fatty acid diesters, have been marketed, and these commercial products can be selected according to the purpose and used in the emulsion composition of the present disclosure.

The emulsion composition of the present disclosure may contain only one type of sucrose saturated fatty acid ester, or may contain two or more types.
The emulsion composition of the present disclosure preferably contains sucrose saturated fatty acid ester in an amount of 0.5% by mass to 10% by mass, and more preferably 1.0% by mass to 5.0% by mass, based on the total amount of the emulsion composition. It is more preferable to contain 1.5 mass%-5.0 mass%.
When the content of sucrose saturated fatty acid ester in the emulsified composition is in the above-mentioned range, the emulsion stability of the emulsified composition, in particular, the reduction inhibitory effect of stability and transparency when applied to a skin external preparation etc. becomes good. .

[Component D: an emulsifier having an unsaturated fatty acid residue]
The emulsion composition of the present disclosure contains at least one emulsifier having an unsaturated fatty acid residue.
Component D is an emulsifier having an unsaturated fatty acid residue as a hydrophobic group.
As unsaturated fatty acid residues in component D, myristoleic acid residues (C14), palmitoleic acid residues (C16), oleic acid residues (C18), linoleic acid residues (C18), linolenic acid residues (C18) Arachidic acid residue (C20), arachidonic acid residue (C20) and the like. In addition, the carbon number of the fatty acid in a fatty acid residue was written together in ().
The unsaturated fatty acid residue in the component D is preferably an unsaturated fatty acid residue selected from oleic acid residue, linoleic acid residue and linolenic acid residue, from the viewpoint of good emulsifying performance.
The hydrophilic portion in component D includes glycerin derivatives (monoglyceryl, polyglyceryl, etc.), sugar derivatives (sucrose monoester, sucrose polyester, sorbitan derivatives, etc.), amino acid derivatives, phospholipid derivatives (glycerophospholipids, sphingophospholipids, etc.) , Polyethylene glycol derivatives, fatty acid salts and the like.
Among them, glycerin derivatives, sucrose derivatives and phospholipid derivatives (glycerophospholipids, sphingophospholipids and the like) are preferable from the viewpoint of biocompatibility.

Although the emulsifier as D component does not have a restriction | limiting in particular except having a unsaturated fatty acid residue, it is preferable to contain at least 1 sort (s) of nonionic emulsifier and amphoteric emulsifier.
As a nonionic emulsifier, a glycerol derivative is preferable from a viewpoint of emulsification performance.
The glycerin derivative may be monoglyceryl or polyglyceryl which is a polymer. Among them, polyglyceryl is preferable, polyglyceryl having an average polymerization degree of 5 or more is more preferable, and polyglyceryl having an average polymerization degree of 5 to 20 is more preferable.

As the amphoteric emulsifier, phospholipid derivatives are preferable from the viewpoint of the emulsification performance.
Phospholipid derivatives are roughly classified into glycerophospholipids having a glycerol skeleton and sphingophospholipids having a sphingosine skeleton. As the component D, glycerophospholipid is more preferable from the viewpoint of the emulsification performance.
Examples of glycerophospholipids include lecithin and lysolecithin, which are a mixture of phosphatidyl choline, phosphatidyl serine, phosphatidyl ethanolamine, phosphatidyl inositol, phosphazic acid and the like. Among them, lecithin is preferable, and soybean-derived lecithin is more preferable.
The glycerophospholipid is preferably an emulsifier having a controlled purity as a glycerophospholipid and the composition of the derivative. Specifically, it is preferable that the glycerophospholipid is a high purity glycerophospholipid having a glycerophospholipid content of 90% by mass or more.
As the composition of glycerophospholipid, it is preferable that lecithin containing phosphatzic acid, phosphatidyl ethanolamine, and phosphatidyl inositol each in an amount of about 10% by mass to 30% by mass is low in terms of good dispersibility. .
The amphoteric emulsifier as the component D preferably has a phosphatidylcholine content in the range of 20% by mass to 32% by mass.
In addition, since hydrogenated lecithin does not have an unsaturated fatty acid residue in a molecule, hydrogenated lecithin (cosmetic display name) is not included in lecithin which is D component of this indication.

The emulsion composition may contain only one type of component D, or may contain two or more types. Among them, it is preferable to contain at least two kinds of the component D from the viewpoint that the emulsifying performance is better.
The emulsified composition preferably contains at least one nonionic emulsifier as component D.
The emulsion composition preferably contains, as component D, at least one amphoteric emulsifier.
It is more preferable that the emulsion composition of the present disclosure contains at least two types of D components, more specifically, at least one type of nonionic emulsifier and at least one type of amphoteric emulsifier.

The ratio of the content of the nonionic emulsifier to the content of the ampholytic emulsifier (the content of the nonionic emulsifier: the content of the ampholytic emulsifier) in the emulsified composition of the present disclosure makes the transparency of the obtained emulsified composition more prolonged From the viewpoint of being able to be maintained, it is preferably 0.01: 1 to 1: 0.01 on a mass basis, and more preferably 0.1: 1 to 1: 0.1.
The emulsion composition contains polyglycerin fatty acid ester as a nonionic emulsifier and lecithin as an amphoteric emulsifier, whereby the transparency of the emulsion composition becomes better, and furthermore, the transparency can be maintained for a longer period of time.

  The content of the component D in the emulsion composition of the present disclosure is preferably 0.1 mass% to 15 mass%, more preferably 1.0 mass% to 10 mass%, and 2.0 mass%. It is further more preferable that it is -7.5 mass%.

[E ingredient: glycerin]
The emulsion composition of the present disclosure contains glycerin.
Glycerin is a kind of polyhydric alcohol and is useful from the viewpoint of control of particle size of oil phase particles, stability, and preservative property.
Moreover, since it has a moisturizing function, a viscosity adjusting function, etc., when an emulsifier is applied to a skin external preparation etc. by containing glycerol, expression of the moisturizing function to skin can be expected.
Glycerin can reduce the interfacial tension between the water phase component and the oil phase component more effectively, and can form finer and more stable fine particles. As a result, for example, in the case of applying the emulsified composition to food applications, it can be expected that intestinal absorbability is enhanced, and in the case of applying to cosmetic applications, transdermal absorbability can be further enhanced.
The content of glycerin is preferably 10% by mass to 60% by mass, more preferably 20% by mass to 55% by mass, and still more preferably 30% by mass to 50% by mass, with respect to the total amount of the emulsion composition.
In the above range, the content of glycerin is preferable in that sufficient storage stability can be easily obtained regardless of the type, content and the like of the oil phase component, and the viscosity of the emulsified composition can be easily suppressed from being high.

[F ingredient: water]
The emulsion composition of the present disclosure contains water from the viewpoint of improving the transparency.
The water used for the emulsified composition is not particularly limited as long as it is water having good biocompatibility, and ultrapure water such as purified water, distilled water, ion exchanged water, pure water, Milli-Q water, etc. Any of the water can be used.
In addition, Milli-Q water is ultra-pure water obtained by Milli-Q water manufacture apparatus which is a maker's ultra pure water manufacture apparatus.
The content of water in the emulsion composition of the present disclosure can be appropriately adjusted according to the intended use of the emulsion composition and the feeling of use required.

[G ingredient: other ingredients]
The emulsion composition of the present disclosure can contain other components in addition to the components A to F described above as long as the effects of the present disclosure can be exhibited.
Hereinafter, other components that can be contained in the emulsion composition of the present disclosure will be described.
[Oil component]
The emulsion composition of the present disclosure can include an oil component. The oil phase particles, which are dispersed particles, contain an oil component.
The oily component that can be used in the emulsion composition is not particularly limited as long as it is a component that dissolves in an oily medium. Among them, carotenoids other than astaxanthin, oils and fats such as coconut oil, and the like can be mentioned.

(Carotenoid)
The emulsion composition of the present embodiment preferably contains a carotenoid containing a natural pigment as an oil component. In addition, astaxanthin which is A component is not contained in the carotenoid as an arbitrary component.
Carotenoids are yellow to red terpenoid pigments and include pigments from plants, algae and bacteria.
Carotenoids are not limited to natural sources, and may be carotenoids obtained according to a conventional method. For example, many of the carotenoid carotenes described below are also produced synthetically, and many of commercially available β-carotene are produced synthetically.

Carotenoids include hydrocarbons (carotene) and their oxidized alcohol derivatives (xanthophylls) and the like.
As carotenoids, actinio erythrol, bixin, canthaxanthin, capsanthin, capsorbin, β-8'-apo-carotenal (apocarotenal), β-12'-apo-carotenal, α-carotene, β-carotene, "carotene" (Mixture of α- and β-carotene), γ-carotene, β-cryptoxanthin, lutein, lycopene, violerythrin, zeaxanthin, and esters of those containing hydroxyl or carboxyl.

Although many of the carotenoids occur naturally in the form of cis and trans isomers, the compounds are often racemic mixtures.
Carotenoids can generally be extracted from plant material. These carotenoids have various functions, for example, lutein extracted from marigold petals is widely used as a raw material for poultry feed, and has a function of coloring the skin and fat of poultry and eggs produced by poultry. is there.

(Oils and fats)
As an oil-based component, fats and oils are mentioned.
As fats and oils as an oil-based component, liquid fats and oils (fatty oil) and solid fats and oils (fat) are mentioned at 25 degreeC.
Examples of liquid oils and fats include olive oil, camellia oil, macadamia nut oil, castor oil, avocado oil, evening primrose oil, turtle oil, corn oil, mink oil, rape seed oil, egg yolk oil, sesame oil, persic oil, wheat germ oil, sasanka Oil, linseed oil, safflower oil, cottonseed oil, Eno oil, soybean oil, peanut oil, tea oil, tea oil, kamin oil, rice bran oil, Japanese oak oil, Japanese tung oil, jojoba oil, germ oil, triglycerin, glycerin trioctanoate, Examples thereof include glycerin triisopalmitate, salad oil, safflower oil (safflower oil), palm oil, coconut oil, peanut oil, almond oil, hazelnut oil, walnut oil, grape seed oil and the like.
Moreover, as solid fats and oils, beef tallow, hardened beef tallow, beef leg fat, beef bone fat, mink oil, egg yolk oil, pork fat, horse fat, sheep fat, hardened oil, cacao butter, coconut oil, hardened coconut oil, palm Oils, hydrogenated palm oil, wax wax, wax kernel oil, hydrogenated castor oil and the like can be mentioned.
Among them, coconut oil, which is a medium-chain fatty acid triglyceride, is preferably used from the viewpoints of the maintenance of the particle diameter of the oil phase particles contained in the emulsified composition and the stability of the oil phase particles.

  When the emulsified composition contains fats and oils, the fats and oils may be contained singly or in combination of two or more.

(Additive)
An emulsion composition can add other additives as needed.
Specific examples of additives that are other components include, for example, anti-acne agents, antiseptics, skin-whitening agents, keratinizers, anti-inflammatory agents, blood circulation enhancers, chelating agents, pH adjusters, pH buffers UV absorbers, perfumes, colorants, organic solvents, preservatives and the like.

[Method for Producing Emulsified Composition]
There are no particular limitations on the method of producing the emulsion composition of the present disclosure.
The emulsion composition of the present disclosure having good transparency and excellent stability over time is preferably produced by the production method described below.

  One example of a method for producing a suitable emulsion composition is to prepare an oil phase composition comprising the component A, the component B, the component D, an amphoteric emulsifier which is a lipophilic component and an optionally contained oily component ( Preparing an aqueous phase composition comprising the step (I), the component C, the component D and the hydrophilic component, the nonionic emulsifier, the component E, the water (component F), and the optionally contained oily component (Step (II)), and emulsifying the mixture of the oil phase composition and the aqueous phase composition under high pressure conditions of 100 MPa or more (step (III)).

<Step (I): Preparation of Oil Phase Composition>
Step (I) comprises obtaining an oil phase composition to be used for preparation of an emulsified composition. In step (I), for example, component A, component B, lipophilic component D, and optionally contained oily component are mixed, heated at 60 ° C. to 100 ° C., and stirred to obtain an oil phase composition. Prepare the

<Step (II): Preparation of Aqueous Phase Composition>
Step (II) comprises preparing an aqueous phase composition to be used for the emulsion composition. In step (II), for example, component C, hydrophilic component D, component E and optionally contained aqueous component are added to water which is component F, heated at 60 ° C. to 90 ° C., and stirred Then, component C and the like are dissolved in water (component F) to prepare an aqueous phase composition.

<Step (III): Preparation of Emulsified Composition>
Step (III) comprises mixing and emulsifying the oil phase composition obtained as described above and the aqueous phase composition to prepare an emulsion composition. In step (III), the aqueous phase composition obtained in each of the above-described steps and the oil phase composition are mixed to obtain a mixture, and the obtained mixture is emulsified under a high pressure condition of 100 MPa or more. I do.
In the case of emulsification treatment, for example, when the emulsification treatment is carried out using a conventional emulsification device utilizing shearing action such as stirrer agitation, impeller agitation, homomixer, continuous flow shearing device under general conditions, Force may be insufficient and fine emulsion particles may not be obtained.
Therefore, the emulsification treatment is preferably performed under high pressure conditions from the viewpoint of the transparency of the obtained emulsified composition and the temporal stability of the gel.
The emulsification treatment under high pressure conditions is preferably an emulsification treatment that imparts a shear force of 100 MPa or more, more preferably 150 MPa or more, and even more preferably 200 MPa or more.

As a stirring means usable for emulsification treatment under high pressure conditions of 100 MPa or more, a high speed stirring method using a homomixer, a disper mixer, an ultra mixer, etc., an ultrasonic method using an ultrasonic homogenizer, a high shear force is applied by a high pressure homogenizer The high pressure homogenizer method etc. are mentioned.
Examples of ultrasonic homogenizers include ultrasonic homogenizers US-600, US-1200T, RUS-1200T, MUS-1200T (above, manufactured by Nippon Seiki Co., Ltd.), ultrasonic processors UIP2000, UIP-4000, UIP-8000. , UIP-16000 (all, manufactured by Hielscher), and the like. The above-mentioned high-power ultrasound system is used at a frequency of 25 kHz or less, preferably 15 kHz to 20 kHz.

Examples of the chamber type high pressure homogenizer include Microfluidizer (manufactured by Microfluidics Co., Ltd.), Nanomizer (manufactured by Yoshida Kikai Kogyo Co., Ltd.), Ultimizer (manufactured by Sugino Machine Co., Ltd.), and the like.
As a homogeneous valve type high pressure homogenizer, a Gorin type homogenizer (manufactured by APV), a Lanier type homogenizer (manufactured by Lanier), a high pressure homogenizer (manufactured by Niro Soavi), a homogenizer (manufactured by Sanwa Kikai Co., Ltd.), a high pressure homogenizer (manufactured by Sanwa Kikai Co., Ltd.) Izumi Food Machinery Co., Ltd. product, an ultra-high pressure homogenizer (manufactured by Squid Co., Ltd.), etc. may be mentioned.
The operating pressure of the high-pressure homogenizer is preferably 100 MPa or more, more preferably 200 MPa or more, from the viewpoint of making the emulsion particles finer.
The number of passes of the high pressure treatment may be one, but from the viewpoint of enhancing the uniformity of the obtained emulsified composition, it is preferably two or more, and more preferably two to five. The temperature of the mixture before the high pressure dispersion treatment is set to 20 ° C to 80 ° C, more preferably 40 ° C to 70 ° C.
Immediately after the high pressure dispersion treatment, it is preferable to rapidly cool the emulsified composition using a cooling means and lower the temperature to a predetermined temperature. As a cooling device, any commercially available heat exchanger can be used.

In the high-pressure emulsification method, the operating conditions of the emulsification device such as the pressure condition and temperature condition described above are not particularly limited because they differ depending on the specifications of the device.
In the manufacturing method as one embodiment described in the present disclosure, the emulsification step may include preliminary emulsification. For example, a pre-emulsification product is obtained by performing emulsification treatment using a conventional emulsifying apparatus utilizing shearing action such as stirrer, impeller stirring, homomixer, continuous flow shearing apparatus, etc. under ordinary conditions, and then pre-emulsification. Further emulsification by high shear treatment under high pressure conditions can be used to obtain a clear gel emulsion composition having the desired fine emulsion particles.
The high shear treatment may be repeated as necessary.
After producing an emulsion in which the oil phase composition is contained at a concentration higher than the concentration in the target final emulsified composition, it can be diluted with water or an aqueous medium to obtain an emulsion composition having a desired concentration.

(Other process)
In the method for producing an emulsified composition, after the emulsified composition is prepared through the above-described steps, a sterilization step can be performed, if necessary.
When the sterilization step is performed, the sterilization step may be performed when preparing the emulsion composition, or may be performed after preparation of the emulsion composition.
When the sterilization step is carried out at the time of preparing the emulsion composition, it may be carried out at any of the above steps. Above all, it is carried out when stirring and mixing the aqueous phase composition and the oil phase composition to obtain a mixture, or carried out as quickly as possible after performing emulsification treatment under high pressure conditions of 100 MPa or more. Is preferred.
According to the method described above, the emulsion composition of the present disclosure having good transparency and excellent temporal stability is produced.

The emulsion composition of the present disclosure can be widely used for external preparations for skin and the like represented by foods and cosmetics. Examples of the food include beverages and frozen desserts, and examples of external skin preparations include skin care cosmetics (such as skin lotions, cosmetic liquids, emulsions, creams, etc.), lipsticks, sunscreen cosmetics, makeup cosmetics and the like. It can, but is not limited to these.
Moreover, in addition to the emulsion composition of the present disclosure described above, it is possible to appropriately add components that can be added to food or cosmetics to foods, skin external preparations and the like to which the emulsion composition is applied. it can.

[Skin external preparation]
The emulsion composition of the present disclosure can be applied to skin external preparations.
The skin external preparation of the present disclosure includes the emulsion composition of the present disclosure described above.
The skin external preparation can further contain a pharmaceutically effective ingredient required as a skin external preparation in addition to the emulsion composition of the present disclosure described above.

The skin external preparation of this indication can apply the emulsion composition of this indication as it is. In this case, it can be expected that the functional components such as astaxanthin which is a functional component contained in the emulsion composition of the present disclosure and glycerin which is a moisturizing component act on the skin.
Furthermore, various medicinal components which can be added to the skin external preparation, a flavor and the like can be appropriately added to the emulsion composition of the present disclosure to make the skin external preparation.

  By incorporating the emulsion composition of the present disclosure into a suitable substrate, it can be made a skin external preparation. That is, the emulsion composition of the present disclosure and a medium that can be a base of at least one external skin preparation may be mixed to form a skin external preparation.

  The content rate of the emulsion composition of the present disclosure in the skin external preparation can be appropriately selected depending on the type, purpose and the like of the product. As described above, the content ratio of the emulsifier in the skin external preparation may be 100% by mass.

In the case of the skin external preparation containing the emulsion composition of the present disclosure and a medium, the content of the emulsion composition in the skin external preparation can be selected according to the purpose.
If an example is given, it can be 0.001 mass%-10 mass% to skin external preparation, Preferably, it is the range of 0.005 mass%-5 mass%.
When the skin external preparation contains the emulsion composition of the present disclosure, it is possible to impart to the skin external preparation a crimson hue having a clearness which the emulsion composition of the present disclosure has. For this reason, the skin external preparation of the present disclosure has a good appearance.

There is no restriction | limiting in particular in the medium which can be used for a skin external preparation, Usually, if it is a medium which can be used for a skin external preparation, it can use arbitrarily.
For example, when an aqueous medium is used, it becomes a lotion-like external preparation for skin that exhibits a clear red appearance.
In addition, when a cream, an ointment, or the like is used as a medium, the external preparation for skin exhibits a crimson appearance.

  Especially as skin external preparations to which the emulsion composition of the present disclosure is suitably applied, cosmetics such as skin care cosmetics (such as skin lotions and cosmetic liquids), body cosmetics (such as body lotions) and scalp cosmetics etc. The fee can be mentioned. Skin external preparations include, in addition to cosmetics, pharmaceuticals for the treatment of skin diseases. However, the application of the emulsion composition of the present disclosure is not limited to the range described above.

The container used for the skin external preparation containing the emulsion composition or the emulsion composition of the present disclosure is not particularly limited.
As the container, a so-called airless container (normal airless container) or a shut-off airless container is preferable from the viewpoint of suppressing the invasion of bacteria from the outside air and enabling the use in an aseptic state for a long time.
In the present disclosure, since glycerin is contained in the emulsified composition or the external preparation for skin, for example, by using a shut-off airless container, the sterilization effect possessed by glycerin is likely to be sustained, and precipitation due to drying generated in the discharge portion , And turbidity can be suppressed.

  There is no restriction | limiting in particular in the manufacturing method of the skin external preparation of this indication, It can manufacture by a well-known method. In addition, when a skin external preparation contains the emulsion composition and medium of this indication, it is preferable to manufacture with the following manufacturing methods.

Preparing an oil-in-water emulsion composition of the present disclosure as described above; and preparing a mixture comprising 0.001% by mass to 10% by mass of the oil-in-water emulsion composition and at least one medium. It is preferable that it is the manufacturing method of the skin external preparation which it has.
Preparation of a mixture can be performed using the stirring and the mixing apparatus suitably selected according to the physical property of a medium, and the content rate of the emulsion composition to be contained.

Hereinafter, although the example which is an example of embodiment of this invention is mentioned and described, it can take various embodiment, unless it is contrary to the main point of this invention.
In addition, unless otherwise indicated, "%" shows the "mass%."

Example 1
The components of the oil phase composition described in Table 1 were dissolved for 1 hour while heating at 70 ° C. to obtain an oil phase composition [step (I)].
The components of the aqueous phase composition described in Table 1 were dissolved for 1 hour while heating at 70 ° C. to obtain an aqueous phase composition [step (II)].

Stirring conditions: 10,000 rpm / min (hereinafter referred to as rpm) using a homogenizer (model name HP 93, manufactured by SMT Co., Ltd.) while maintaining the aqueous phase composition at 70 ° C. The resulting oil phase composition was added to the aqueous phase composition obtained above and stirring was continued for 1 minute to perform pre-emulsification.
Subsequently, high pressure emulsification was carried out at a pressure of 245 MPa using Ultimizer HJP-25005 (manufactured by Sugino Machine Co., Ltd.).
Then, it filtered with the microfilter of 1 micrometer of average hole diameters, and prepared the emulsion composition (EM-1) of the Example.
The emulsion compositions (EM-2) to (EM-6) of the examples were prepared according to the formulation described in Table 1 in the same manner as the preparation of the emulsion composition (EM-1).
Moreover, it carried out similarly to preparation of an emulsion composition (EM-1), and according to the prescription of Table 1, comparative emulsion compositions (T-1)-(T-3) were prepared.

The detail of the component used for the emulsion composition of each Example, and a comparative emulsion composition is as follows.
[A component]
Hematococcus alga extract (astaxanthin content 20% by mass, ASTOTS-S, manufactured by Fujifilm Corporation)
[B component]
・ Tocopheryl nicotinate (tocopherol nicotinate, Eisai Food Chemical Co., Ltd.)
[C component]
・ Sucrose stearic acid ester (monoester content: 75%, Ryoto (registered trademark) sugar ester S-1670, manufactured by Mitsubishi Chemical Foods Co., Ltd.)

[D component]
-Polyglyceryl oleate (NIKKOL (registered trademark) Decaglyn 1-O, manufactured by Nikko Chemicals Co., Ltd.)
-Polyglyceryl oleate (Sansoft A-171E-C, manufactured by Solar Chemical Co., Ltd.)
-Polyglyceryl stearate (NIKKOL (registered trademark) Decaglyn 1-SV, manufactured by Nikko Chemicals Co., Ltd.)
・ Lecithin A (SLP white, manufactured by Fuso Oil Co., Ltd.)
・ Lecithin B (SLP-PC70, manufactured by Fuso Oil Co., Ltd.)
· Hydrogenated lecithin (SLP-White H, manufactured by Fusa Oil Co., Ltd.)
[E component]
-Glycerin (food additive glycerin, manufactured by Kao Corporation)
[Other ingredients]
・ Mix tocopherol (RIKEN E oil 800, manufactured by Riken Vitamin Co., Ltd.)
・ Coconut oil (made by O.D.O. Nisshin OilliO Group Ltd.)

Example 2
According to the composition described in Table 2, cosmetics 1-1 to 1-6 and comparative cosmetics C1-1 to C1-3 as skin external preparations were prepared by a conventional method. The total amount is 100% by mass.

The B. cerebella dispersion described in cosmetic composition 1 and the Boswellia cerata dispersion were prepared by subjecting the following dispersion to a high pressure emulsification treatment in the same manner as in Example 1.
(Centrifuge dispersion) [Content (% by mass)]
TECA (product name) (Bayer Healthcare Co., Ltd.) 1.0
Lecithin (SLP White: Fuso Oil Co., Ltd.) 5.0
Glycerin 45.0

(Boswellia Serrata Dispersion) [Content (% by mass)]
Boswellia Serrata Extract (Boswellin CG: trade name, Sabinsa Japan Corporation Ltd.) 1.0
POE (20) Phytosterol Ether (NIKKOL BPS-20)
3.75
1,3-butylene glycol 15.25
Lecithin (SLP White: Fuso Oil Co., Ltd.) 5.0
Glycerin (alcohol) 45.0
Purified water Upto100

[Skin external preparation: cosmetic composition 1] [content (% by mass)]
Phosphoric acid-L-Ascorbyl magnesium 2.0
Glycyrrhizinate dipotassium 1.0
Dipropylene glycol 4.0
Glycerin 5.0
Diglycerin 2.0
1,2-Pentanediol 2.0
Phenoxyethanol 0.5
Methyl parahydroxybenzoate 0.1
Alkaline Necessator B-16 polymer 0.01
Polyoxyethylene Phytosterol (NIKKOL®)
BPS-20: Nikko Chemicals Co., Ltd. product 0.01
Polyoxyethylene Cured Castor Oil (60E.O.) 0.1
Aroma dispersion 0.5
Boswellia Serrata Dispersion 0.5
Any one of the emulsion composition prepared in Example 1 or the comparative emulsion compositions (EM-1 to EM-6) and (T-1 to T-3) 0.4
Citric acid 0.7
Sodium citrate appropriate amount (pH adjusted to 7.4)
N-acetyl-L-hydroxyproline 0.5
Apple fruit culture cell extract 0.5
Water soluble collagen 0.5
Hydrolyzed collagen 0.5
Yeast extract (1) 0.5
Water remaining amount

[Evaluation]
The following evaluation was implemented with respect to each obtained cosmetic. The results are listed in Table 2.
1. Transparency The obtained cosmetic was diluted to 1% by mass with pure water to prepare a measurement sample. The absorbance at 625 nm of the measurement sample was measured by a spectrophotometer (V-630, JASCO Corporation) using a cell with an optical path length of 1 cm, and this was used as an index of transparency. From the viewpoint of marketability, 0.20 or less is an acceptable range.

2. Storage stability The obtained cosmetic was stored for 2 weeks in an environment of 50 ° C., and then diluted to 1% by mass with pure water to prepare a measurement sample. Measure the absorbance at 625 nm of the measurement sample using a cell with an optical path length of 1 cm with a spectrophotometer (V-630, JASCO Corporation), store the initial absorbance at 625 nm (numerical value of transparency), and after storage The difference between the value of the absorbance and the value of the absorbance was used as an indicator of storage stability. From the viewpoint of merchandiseability, it is an allowable range that the difference from the initial value (transparency) is 0.20 or less.

3. Light resistance The obtained cosmetic was filled in a quartz cell, and irradiated with light of illuminance 30,000 Lux for 144 hours with a white fluorescent lamp. The sample was measured with a spectrophotometer (V-630, Nippon Bunko Co., Ltd.) for the absorbance at 475 nm before irradiation and the absorbance at 475 nm after irradiation, and the residual ratio was calculated based on the following formula, and light resistance (astaxanthin As an indicator of
The sample was prepared by diluting the cosmetic before and after irradiation to 1% by mass with pure water.
From the viewpoint of marketability, the residual rate of 70% or more is an acceptable range.
Residual rate (%) = [(absorbance at 475 nm after irradiation) / (absorbance at 475 nm before irradiation)] × 100

As is clear from Table 2, the cosmetic compositions 1-1 to 1-6 using the emulsion compositions EM-1 to EM-6 of the examples all have initial transparency and storage stability (transparency) Change) was good. Further, it can be seen that the deterioration of the appearance due to the color fading due to light is suppressed because the light resistance is good.
On the other hand, the comparative cosmetics of C1-1 to C1-3 using the comparative emulsion composition are inferior in initial transparency, and particularly the comparative cosmetic C1-2 not containing the component B has poor light resistance. From the above, it can be seen that light has faded.

[Example 3]
Composition 1 having the composition of Table 3 was prepared by subjecting it to the high pressure emulsification treatment in the same manner as Example 1.
Furthermore, apple fruit culture cell extract, Boswellia serrata dispersion, an emulsified composition and purified water are added to the composition 1 after high-pressure emulsification processing in an amount to be the following cosmetic composition 2, and the cosmetic (2-1 ) To (2-6) and comparative cosmetics (C2-1) to (C2-3) were prepared.

[Skin external preparation: cosmetic composition 2] [content (% by mass)]
Composition 1 (Composition 1 described in Table 3) 97
Apple fruit culture cell extract 0.5
Boswellia Serrata Dispersion 0.5
Any one of the emulsion compositions (EM (1-1) to (1-6)) and comparative emulsion compositions (T-1) to (T-3) prepared in Example 1 0.2
Purified water Upto100

[Evaluation]
Evaluation was carried out in the same manner as the cosmetic obtained in Example 2 for each cosmetic obtained. The results are shown in Table 4.

As apparent from Table 4, the cosmetics using the emulsion compositions EM-1 to EM-6 in the examples had initial transparency and preservation even when the formulation of the medium used in combination was different. The stability (change in transparency) was good. Further, it can be seen that the deterioration of the appearance due to the color fading due to light is suppressed because the light resistance is good.
On the other hand, the cosmetic using the comparative emulsion composition is inferior in initial transparency, and in particular, the comparative cosmetic C2-2 which does not contain the component B is deteriorated in light resistance because of poor light resistance. Recognize.

Example 4
The components of the aqueous phase composition described in Table 5 were dissolved for 1 hour while heating at 70 ° C. to obtain an aqueous phase composition.
The components of the oil phase composition described in Table 5 were dissolved for 1 hour while heating at 70 ° C. to obtain an oil phase composition.
The above aqueous phase composition is stirred with a homogenizer (model name HP 93, manufactured by SMT Co., Ltd.) while keeping the aqueous phase composition at 70 ° C. (10000 rpm), the above obtained oil phase composition is added, and stirring is continued for 1 minute Pre-emulsified.
Subsequently, high pressure emulsification was carried out at a pressure of 245 MPa using Ultimizer HJP-25005 (manufactured by Sugino Machine Co., Ltd.).
Thereafter, the resultant was filtered with a microfilter having an average pore diameter of 1 μm to prepare emulsion compositions EM-7 to EM-12 of the example and comparative emulsion compositions T-4 to T-6.

[Example 5]
Cosmetics 3-1 to 3-6 and comparative cosmetics C3-1 to C3-3 having the following compositions were prepared by a conventional method (total amount: 100% by mass).
The Centella caliber dispersion, Boswellia serrata dispersion in composition is the dispersion prepared in Example 2.
[Skin external preparation: cosmetic composition 3] [content (% by mass)]
Phosphoric acid-L-Ascorbyl magnesium 2.0
Glycyrrhizinate dipotassium 1.0
Dipropylene glycol 4.0
Glycerin 5.0
Diglycerin 2.0
1,2-Pentanediol 2.0
Phenoxyethanol 0.5
Methyl parahydroxybenzoate 0.1
Alkaline Necessator B-16 polymer 0.01
Polyoxyethylene Phytosterol (NIKKOL BPS-20: manufactured by Nikko Chemicals Co., Ltd.) 0.01
Polyoxyethylene Cured Castor Oil (60E.O.) 0.1
Aroma dispersion 0.5
Boswellia Serrata Dispersion 0.5
Emulsified composition (EM-7) to (EM-12) prepared in Example 4;
Comparative emulsion composition (T-4) to any one of (T-6) 0.4
Citric acid 0.7
Sodium citrate appropriate amount (pH adjusted to 7.4)
N-acetyl-L-hydroxyproline 0.5
Apple fruit culture cell extract 0.5
Water soluble collagen 0.5
Hydrolyzed collagen 0.5
Yeast extract (1) 0.5
Water remaining amount

  The cosmetic obtained in Example 5 was evaluated in the same manner as the cosmetic obtained in Example 2. The results are shown in Table 6 below.

As is clear from Table 6, the cosmetics using the emulsion compositions EM-7 to EM-12 of the examples all have initial transparency even when the content of the emulsion composition is increased. And storage stability (change in transparency) was good. Further, it can be seen that the deterioration of the appearance due to the color fading due to light is suppressed because the light resistance is good.
On the other hand, the cosmetic using the comparative emulsion composition is inferior in initial transparency, and in particular, the cosmetic of Comparative Example (C3-2) not containing the component B is discolored by light because of poor light resistance. I understand that.

  From the above results, it was found that the emulsion compositions of the examples had good transparency in the initial and after storage, and good light resistance, while containing astaxanthin. For this reason, it is confirmed that the cosmetic which is an external preparation for skin containing the emulsified composition is maintained for a long time without fading the appearance of transparency and the red color of astaxanthin, and that the stability of the appearance and the appearance is good It was done.

Claims (10)

An oil-in-water emulsion composition comprising components A to F.
A: astaxanthin B: tocopherol nicotinate C: sucrose saturated fatty acid ester D: at least one emulsifier selected from emulsifiers having unsaturated fatty acid residues E: glycerin F: water   The oil-in-water emulsion composition according to claim 1, wherein the unsaturated fatty acid residue in the component D is an unsaturated fatty acid residue selected from oleic acid residue, linoleic acid residue and linolenic acid residue.   The oil-in-water emulsion composition according to claim 1 or claim 2, which contains at least two kinds of component D.   The oil-in-water type emulsion composition according to any one of claims 1 to 3, wherein the component D contains a nonionic emulsifier.   The oil-in-water emulsion composition according to claim 4, wherein the nonionic emulsifier comprises polyglyceryl.   The oil-in-water emulsion composition according to claim 5, wherein the average degree of polymerization of polyglyceryl is 5-20.   The oil-in-water type emulsion composition according to any one of claims 1 to 6, wherein the D component contains an amphoteric emulsifier.   The oil-in-water emulsion composition according to claim 7, wherein the amphoteric emulsifier comprises lecithin.   The skin external preparation containing the oil-in-water type emulsion composition of any one of Claims 1-8. A process of preparing the oil-in-water emulsion composition according to any one of claims 1 to 8.
Preparing a mixture comprising 0.001% by weight to 10% by weight of the oil-in-water emulsion composition and at least one medium;
The manufacturing method of the skin external preparation which has.