JP2018177695A - Soybean rust disease control method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an effective controlling method for soybean rust.SOLUTION: A soybean rust controlling method includes applying a cartap hydrochloride, and a compound selected from the following group (A) to soybean or a cultivation area of soybean. Group (A): manzeb, chlorothalonil, cyproconazole, epoxiconazole, tebuconazole, and prothioconazole.SELECTED DRAWING: None

Description

  The present invention relates to a method for controlling soybean rust.

  Soybean rust is known to cause enormous damage to soybean production (see, for example, Non-Patent Document 1).

J. T. Yorinori, 7 others, Plant Disease, (US), The American Phytopathological Society, June 2005, p. 675-677

  An object of the present invention is to provide an effective control method against soybean rust.

The present inventors exert excellent control efficacy against soybean rust by applying cartap hydrochloride and a compound selected from the following group (A) to soybeans or soybean cultivation areas by a specific method I found out.
That is, the present invention is as follows.
[1] A method for controlling soybean rust, which comprises applying cartap hydrochloride and a compound selected from the following group (A) (hereinafter referred to as the present compound A) to soybeans or a cultivated land of soybeans.
Group (A): Manzeb, chlorothalonil, cyproconazole, epoxiconazole, tebuconazole, and prothioconazole.
[2] The soybean rust disease according to [1], which comprises the steps of mixing cartap hydrochloride, the present compound A and water to prepare a spray solution, and applying the spray solution to soybeans or a soybean cultivation area Control method.
[3] The method for controlling soybean rust according to [2], wherein the step of applying the spray solution is a step of applying to a foliage of soybean.
[4] The weight ratio of cartap hydrochloride to the present compound A is cartap hydrochloride / the present compound A = 500/1 to 0.05 / 1, described in any one of [1] to [3] Soybean rust disease control method.
[5] The weight ratio of cartap hydrochloride to the present compound A is cartap hydrochloride / the present compound A = 50/1 to 0.1 / 1, described in any one of [1] to [3] Soybean rust disease control method.
[6] [1] to [1], wherein the weight ratio of cartap hydrochloride to the present compound A is Cartap hydrochloride / the present compound A = 20/1 to 5/1, or 1/1 to 0.2 / 1. The method for controlling soybean rust according to any one of 3).
[7] The compound A is a compound selected from manzeb and chlorothalonil, and the weight ratio of cartap hydrochloride to a compound selected from manzeb and chlorothalonil is a compound selected from cartap hydrochloride / manzeb and chlorothalonil = 1/1 The method for controlling soybean rust according to any one of [1] to [3], which is ~ 0.2 / 1.
[8] The compound A is a compound selected from cyproconazole, epoxiconazole, tebuconazole and prothioconazole, and is selected from cartap hydrochloride and a compound selected from cyproconazole, epoxiconazole, tebuconazole and prothioconazole The compound according to any one of [1] to [3], wherein the weight ratio of is: compound selected from cartap hydrochloride / cyproconazole, epoxiconazole, tebuconazole, and prothioconazole = 20/1 to 5/1. The soybean rust disease control method as described.
[9] The method for controlling soybean rust according to any one of [1] to [8], wherein the total application rate of cartap hydrochloride and the present compound A is 100 to 2,000 g per hectare.
[10] The method for controlling soybean rust according to any one of [2] to [9], wherein 1 to 1,000 L of the spray solution is applied per hectare.
[11] The soybean rust according to any one of [2] to [10], which comprises adding an adjuvant component selected from the group consisting of mineral oil, vegetable oil methyl ester and silicone in the step of preparing a spray solution. Disease control method.
[12] The method according to [11], wherein the concentration of the adjuvant component in the spray solution is 50 to 20,000 mg / L.
[13] The method according to any one of [1] to [12], wherein cartap hydrochloride and the present compound A are applied in the period from the first double leaf stage (V1 phase) to the mature phase (R8 phase) of soybean Soybean rust disease control method.
[14] The method for controlling soybean rust according to any one of [1] to [13], wherein the soybean is soybean to which soybean rust resistance has been imparted.
[15] The method for controlling soybean rust according to any one of [1] to [14], wherein the soybean is a genetically modified soybean.

  According to the present invention, soybean rust can be controlled.

で示される、１,３−ビス(カルバモイルチオ)−２−(Ｎ,Ｎ−ジメチルアミノ)プロパン塩酸塩のことをいう。該化合物は、Ｔｈｅ Ｐｅｓｔｉｃｉｄｅ Ｍａｎｕａｌ １７ｔｈ ｅｄｉｔｉｏｎの１７０−１７１ページに記載されており、市販品（例えば、パダンＳＧ水溶剤）として入手可能である。また、公知の方法により製造することもできる。
本発明に用いるマンゼブは、Ｔｈｅ Ｐｅｓｔｉｃｉｄｅ Ｍａｎｕａｌ １７ｔｈ ｅｄｉｔｉｏｎの６８９−６９１ページに記載されており、市販品として入手可能である。また、公知の方法により製造することもできる。
本発明に用いるクロロタロニルは、Ｔｈｅ Ｐｅｓｔｉｃｉｄｅ Ｍａｎｕａｌ １７ｔｈ ｅｄｉｔｉｏｎの１９６−１９８ページに記載されており、市販品として入手可能である。また、公知の方法により製造することもできる。
本発明に用いるシプロコナゾールは、Ｔｈｅ Ｐｅｓｔｉｃｉｄｅ Ｍａｎｕａｌ １７ｔｈ ｅｄｉｔｉｏｎの２７７−２７８ページに記載されており、市販品として入手可能である。また、公知の方法により製造することもできる。
本発明に用いるエポキシコナゾールは、Ｔｈｅ Ｐｅｓｔｉｃｉｄｅ Ｍａｎｕａｌ １７ｔｈ ｅｄｉｔｉｏｎの４０９−４１０ページに記載されており、市販品として入手可能である。また、公知の方法により製造することもできる。
本発明に用いるテブコナゾールは、Ｔｈｅ Ｐｅｓｔｉｃｉｄｅ Ｍａｎｕａｌ １７ｔｈ ｅｄｉｔｉｏｎの１０５４−１０５６ページに記載されており、公知の方法により製造することができる。
本発明に用いるプロチオコナゾールは、Ｔｈｅ Ｐｅｓｔｉｃｉｄｅ Ｍａｎｕａｌ １７ｔｈ ｅｄｉｔｉｏｎの９４４−９４５ページに記載されており、市販品として入手可能である。また、公知の方法により製造することもできる。 Cartap hydrochloride used in the present invention has the following formula:

It refers to 1,3-bis (carbamoylthio) -2- (N, N-dimethylamino) propane hydrochloride represented by The compound is described on pages 170-171 of The Pesticide Manual 17th edition, and is available as a commercial product (for example, Padang SG water solvent). Moreover, it can also manufacture by a well-known method.
Manzeb used in the present invention is described on pages 689-691 of The Pesticide Manual 17th edition, and is commercially available. Moreover, it can also manufacture by a well-known method.
Chlorothalonil used in the present invention is described on pages 196 to 198 of The Pesticide Manual 17th edition, and is commercially available. Moreover, it can also manufacture by a well-known method.
Ciproconazole used in the present invention is described on pages 277-278 of The Pesticide Manual 17th edition, and is commercially available. Moreover, it can also manufacture by a well-known method.
The epoxyconazole used in the present invention is described on pages 409-410 of The Pesticide Manual 17th edition, and is commercially available. Moreover, it can also manufacture by a well-known method.
Tebuconazole used in the present invention is described on pages 1054-1056 of The Pesticide Manual 17th edition, and can be produced by a known method.
Prothioconazole used in the present invention is described on pages 944 to 945 of The Pesticide Manual 17th edition, and is commercially available. Moreover, it can also manufacture by a well-known method.

  In the present invention, the weight ratio of cartap hydrochloride to the present compound A is usually cartap hydrochloride / the present compound A = 500/1 to 0.05 / 1, preferably, cartap hydrochloride / the present compound A = 50 / More preferably, it is Cartap hydrochloride / the present compound A = 50/1 to 5/1, or 1/1 to 0.1 / 1. Specifically, for example, the weight ratio of cartap hydrochloride to a compound selected from manzeb and chlorothalonil is compound selected from cartap hydrochloride / manzeb and chlorothalonil = 1/1 to 0.2 / 1. Also, for example, the weight ratio of cartap hydrochloride to a compound selected from cyproconazole, epoxiconazole, tebuconazole and prothioconazole is from cartap hydrochloride / ciproconazole, epoxiconazole, tebuconazole and prothioconazole The compound to be selected is 20/1 to 5/1.

In the present invention, it is preferable to use a composition containing cartap hydrochloride and the present compound A (hereinafter referred to as the present composition). Although the present composition may be a mixture of cartap hydrochloride and the present compound A itself, the present composition generally comprises a mixture of cartap hydrochloride, the present compound A and an inert carrier, and optionally a surfactant or the like. Other pharmaceutical auxiliaries are added and formulated into oils, emulsions, flowables, wettable powders, water dispersible granules, powders, granules and the like. Such formulations can be used as pest control agents as such or with the addition of other inactive ingredients.
In the present composition, cartap hydrochloride and the present compound A are generally contained in an amount of usually 0.1 to 99% by weight, preferably 0.2 to 90% by weight, more preferably 1 to 80% by weight.

Inert carriers used in formulation include solid carriers and liquid carriers, and examples of solid carriers include kaolin clay, attapulgite clay, bentonite, montmorillonite, acid clay, pyrophyllite, talc, diatomaceous earth Fine powder or particles of mineral such as calcite, natural organic matter such as corn cob powder, walnut shell powder, synthetic organic matter such as urea, salts such as calcium carbonate and ammonium sulfate, synthetic inorganic matter such as synthetic hydrous silicon oxide etc. Can be mentioned. Further, as the liquid carrier, for example, aromatic hydrocarbons such as xylene, alkylbenzene and methylnaphthalene, alcohols such as 2-propanol, ethylene glycol, propylene glycol and ethylene glycol monoethyl ether, and ketones such as acetone, cyclohexanone and isophorone And vegetable oils such as soybean oil and cottonseed oil, petroleum aliphatic hydrocarbons, esters, dimethyl sulfoxide, acetonitrile and water.
As surfactant, for example, anionic interface such as alkyl sulfate ester salt, alkyl aryl sulfonate, dialkyl sulfo succinate, polyoxyethylene alkyl aryl ether phosphate ester, lignin sulfonate, naphthalene sulfonate formaldehyde polycondensate, etc. Activators and nonionic surfactants such as polyoxyethylene alkyl aryl ethers, polyoxyethylene alkyl polyoxypropylene block copolymers, sorbitan fatty acid esters, and cationic surfactants such as alkyltrimethyl ammonium salts can be mentioned.
Other pharmaceutical adjuvants include, for example, water-soluble polymers such as polyvinyl alcohol and polyvinyl pyrrolidone, gum arabic, alginic acid and salts thereof, CMC (carboxymethyl cellulose), polysaccharides such as xanthan gum, aluminum magnesium silicate, alumina sol And inorganics, preservatives, coloring agents and stabilizers such as PAP (isopropyl acid phosphate), BHT and the like.

  The present composition is also prepared by mixing each preparation obtained by formulating cartap hydrochloride and the present compound A respectively according to the method described above, or mixing each mixed solution obtained by mixing these preparations with water respectively. (For example, in the tank of a spreader).

  The composition may further contain one or more other bactericidal agents.

  As an aspect of this composition, the following are mentioned, for example.

Examples of the combination of Cartap hydrochloride and the present compound A include the combinations described below.
Combination of Cartap hydrochloride and Manzeb;
A combination of cartap hydrochloride and chlorothalonil;
Combination of cartap hydrochloride and cyproconazole;
A combination of cartap hydrochloride and epoxiconazole; and
Combination of cartap hydrochloride and prothioconazole.

In the present invention, it is preferable to apply a spray prepared by mixing cartap hydrochloride and the present compound A with water to a soybean or soybean cultivation area. In the spray solution, the combination of Cartap hydrochloride and the present compound A includes the following embodiments.
Spray solution containing cartap hydrochloride and manzeb in a weight ratio of cartap hydrochloride / manzeb = 1/1 to 0.1 / 1;
Spray solution containing cartap hydrochloride and chlorothalonil in a weight ratio of cartap hydrochloride / chlorothalonil = 1/1 to 0.1 / 1;
Spray solution containing cartap hydrochloride and cyproconazole in a weight ratio of cartap hydrochloride / ciproconazole = 50/1 to 5/1;
Spray solution containing cartap hydrochloride and epoxyconazole in a weight ratio of cartap hydrochloride / epoxyconazole = 50/1 to 5/1;
A spray solution containing cartap hydrochloride and tebuconazole in a weight ratio of cartap hydrochloride / tebuconazole = 50/1 to 5/1;
A spray solution containing cartap hydrochloride and prothioconazole in a weight ratio of cartap hydrochloride / prothioconazole = 50/1 to 5/1.

  The method for controlling soybean rust of the present invention (hereinafter referred to as the controlling method of the present invention) has a step of applying effective amounts of cartap hydrochloride and the present compound A to soybean or a cultivated area of soybean. In the step, the total application rate of cartap hydrochloride and the present compound A is usually 100 to 2,000 g, preferably 250 to 2,000 g, per hectare of soybean field.

  Forms of application include, for example, foliage treatment and soil treatment.

As the foliage treatment, for example, a method of spraying on the foliage of cultivated soybean is mentioned.
The soil treatment includes, for example, soil spraying, soil mixing, and chemical solution irrigation to the soil.

  In a preferred embodiment of the control method of the present invention, following the step of mixing cartap hydrochloride, the present compound A and water to prepare a spray solution, the step of applying the spray solution to soybeans or a soybean cultivation area is carried out. Do. In the present specification, the application liquid means a liquid prepared by mixing cartap hydrochloride, the present compound A and water for application. The spray solution optionally contains an adjuvant component. Preferably, in the step of preparing the spray solution, one or more adjuvant components selected from the group consisting of mineral oil, vegetable oil methyl ester and silicone are added. In the present specification, the adjuvant component means a component that helps the pesticidal active ingredient to exert its control efficacy. In addition, in the present specification, those prepared in a form in which an adjuvant component can be added to water are simply referred to as an adjuvant. The mineral oil is preferably liquid paraffin (CAS No. 8012-95-1), solvent refined heavy paraffinic distillate (CAS No. 64741-88-4) or solvent refined light paraffinic distillate (CAS No. 64741-89-5). Vegetable oil methyl ester is an ester produced by methyl esterification of vegetable oil. The vegetable oil methyl ester is preferably soybean oil methyl ester. The silicone is preferably a polyether modified silicone. The polyether modified silicone is a silicone having a polyether chain, which is produced by adding a polyether having a reactive unsaturated group to an organohydrogensiloxane. The polyether chain is composed of ethylene oxide, propylene oxide or both. The preferred adjuvant component is liquid paraffin (CAS No. 8012-95-1), solvent refined light paraffinic distillate oil (CAS No. 64741-89-5), soybean oil methyl ester and polyether modified silicone One or more adjuvant components to be selected. The adjuvant containing liquid paraffin (CAS No. 8012-95-1) as an adjuvant component includes NIMBUS (registered trademark). Examples of the adjuvant containing a solvent-refined light paraffinic distillate oil (CAS No. 64741-89-5) as an adjuvant component include ASSIST (registered trademark). As an adjuvant containing soybean oil methyl ester as an adjuvant component, AUREO (registered trademark) can be mentioned. Adjuvants containing polyether modified silicone as adjuvant component include BREAK-THRU® and SILWET L-77®.

  In the step of preparing the spray solution, it is preferable to use cartap hydrochloride and the present compound A in a dosage form to be applied mixed with water. As such a dosage form, for example, wettable powders, water dispersible granules, water solvents, granular water solvents can be mentioned. In the step of preparing the spray solution, cartap hydrochloride, the present compound A and water are mixed. Preferably, the present composition and water are mixed. Specifically, the present composition is usually prepared using 0.1 to 3,000 g, preferably 0.5 to 1,000 g, per 1 L of water. When an adjuvant is added in the step of preparing the spray solution, the concentration of the adjuvant component in the spray solution is usually 50 to 20000 mg, preferably 75 to 20,000 mg / L or 75 to 5,000 mg / L. Add the adjuvant as you like. When an adjuvant is added, the spray solution may be prepared by mixing the composition and water and then adding an adjuvant, or after adding an adjuvant to water, adding the composition and mixing May be prepared.

  In the step of applying the spray solution to soybeans or soybean cultivation areas, the spray solution is generally applied in an amount of 1 to 1,000 L, preferably 30 to 500 L, per hectare of soybean field. The mode of application is preferably stem and leaf treatment.

  In the control method of the present invention, cartap hydrochloride and the present compound A are generally applied to soybeans or soybean cultivation areas in a period from the first double leaf stage (V1 phase) to the mature phase (R8 phase) of soybean. The application time is preferably a period from the fifth double leaf stage (V5) to the seedling hypertrophy stage (R6 stage) of soybean, more preferably for the seventh double leaf stage (V7 stage) to the early stage of seednut hypertrophy (R5 stage) Period). The growth stage in soybean consists of a vegetative growth period consisting of VE, VC and Vn, and a reproduction growth period consisting of R1 to R8. In the vegetative growth period, VE is the rooting stage and VC is the cotyledon spreading stage. Vn is the n-th multiple leaf stage, and n represents an integer of 1 or more. Specifically, V1 is a first multiple leaf stage, V2 is a second multiple leaf stage, and V3 is the third multiple leaf stage. R1 during flowering, R2 during flowering, R3 during brow elongation, R4 during brow elongation, R5 during brow elongation, R5 during grain hypertrophy, R6 during grain hypertrophy, R7 during early maturation, R8 during maturation It is.

  The soybean may be soybean that has been bred by hybrid technology. The soybeans bred by the hybrid technology are one-time hybrids obtained by crossing two different lines of breeds, and are generally soybeans having the characteristics of heterosis with traits superior to either of the parents.

  The soybean may be soybean that has been modified using genetic engineering techniques. As such soybean, for example, the genetically modified crop registration database (GM) of the website (http://www.isaaa.org/) of the International Agribio Agency (INTERNATINAL SERVICE for the ACQUISITION of AGRI-BIOTECH APPLICATIONS, ISAAA) Examples include soybean listed in APPROVAL DATABASE). More specifically, soybeans to which environmental stress resistance, disease resistance, herbicide resistance, pest resistance and the like have been imparted by genetic engineering, or traits related to growth and yield, quality of products, fertility traits and the like. It may be modified soybeans.

In soybeans to which herbicide resistance has been imparted by genetic engineering technology, protoporphyrinogen oxidase such as flumioxazin, 4-hydroxyphenylpyruvate dioxygenase such as isoxaflutole, mesotrione (hereinafter referred to as HPPD) ) Inhibitors, acetolactate synthetase (hereinafter referred to as ALS) inhibitors such as imazethapyr, thifensulfuron methyl, etc. 5-enolpyruvyl shikimate 3-phosphate synthase (hereinafter referred to as EPSP) inhibitions such as glyphosate etc. Also included are soybeans to which tolerance to herbicides such as agents, glutamine synthetase inhibitors such as glufosinate, auxin type herbicides such as 2,4-D, dicamba, and bromoxynil have been imparted by genetic engineering techniques.
Glyphosate resistant EPSPS gene (CP4 epsps) derived from Agrobacterium tumefaciens strain CP4 as an example of soybean which has been rendered herbicide resistant by genetic recombination technology, derived from Bacillus licheniformis (Bacillus licheniformis) Glyphosate metabolizing enzyme gene (gat 4601, gat 6421) whose metabolic activity has been enhanced by shuffling the glyphosate metabolizing enzyme (glyphosate N-acetyltransferase) gene, glyphosate metabolizing enzyme (glyphosate enzyme from Ochrobacterum anthropi strain LBAA) There is a glyphosate-resistant genetically modified soybean into which one or more of an oxidase gene, goxv 247) or an EPSPS gene (mepsps, 2 mepsps) having a glyphosate-resistant mutation derived from corn or the like are introduced. A glyphosate-resistant soybean into which an EPSPS gene derived from Agrobacterium tumefaciens strain CP4 has been introduced is commercially available under the trade name of Genuity (registered trademark) Roundup Ready 2 Yield (registered trademark) Soybean.
Similarly, phosphinothricin N-acetyltransferase (Phosphinothricin N-), which is a glufosinate-metabolizing enzyme derived from Streptomyces hygroscopicus, as an example of soybeans to which herbicide resistance has been imparted by genetic engineering techniques. acetyltransferase (PAT) gene (bar), phosphinothricin N-acetyltransferase gene (pat), which is a glufosinate-metabolizing enzyme derived from Streptomyces viridochromogenes, or a synthesized pat gene, etc. There is introduced glufosinate-resistant transgenic soybean. A genetically modified soybean that expresses glufosinate metabolizing enzymes (bar, pat) derived from Streptomyces bacteria is sold under the trade name LibertyLink® Soybean.
Similarly, as an example of soybeans to which herbicide resistance has been imparted by genetic engineering technology, a genetically modified soybean into which an ALS herbicide resistant type ALS gene (csr1-2) derived from Arabidopsis thaliana has been introduced is the name of Culture®. Is being developed. Furthermore, there is a genetically modified soybean that is resistant to a sulfonylurea herbicide into which a soybean-derived ALS herbicide resistant ALS gene (gm-hra) has been introduced.
Similarly, as an example of soybeans to which herbicide resistance has been imparted by genetic engineering technology, isoxaflutole resistance gene pairs introduced with HPPD herbicide resistant HPPD gene (hppdPFW336) derived from Pseudomonas fluorescens strain A32 There are modified soybeans and mesotrione resistant transgenic soybeans into which the HPPD gene (avhppd-03) derived from oat (Avena sativa) has been introduced.
Similarly, as an example of soybeans to which herbicide resistance has been imparted by genetic engineering techniques, aryloxyalkanoate dioxygenase, which is a 2,4-D metabolizing enzyme derived from Sphingobium herbididovorans, is known. ) 2,4-D resistance into which the gene (aad-1) or the allyloxyalkanoate dioxigenase gene (aad-12) which is a 2,4-D metabolizing enzyme derived from Delftia acidovorans is introduced Some of them are developed under the trade name Enlist® Soybean. In addition, there is a dicamba-resistant genetically modified soybean into which a dicamba monooxygenase (Dicamba monooxygenase) gene (dmo), which is a dicamba metabolic enzyme derived from Stenotrophomonas maltophilia strain DI-6, has been introduced.
Similarly, as an example of soybean that has been rendered resistant to two or more herbicides by genetic engineering, a genetically modified soybean that is resistant to both glufosinate and 2,4-D is Enlist® Soybean. It has been developed under the trade name. A genetically modified soybean that is resistant to both glyphosate and dicamba is being developed under the trade name Genuity® Roundup Ready® 2 Xtend®. Genetically modified soybeans that are resistant to both glyphosate and ALS inhibitors are being developed under the tradename Optimum® GAT®. In addition, genetically modified soybeans that are resistant to both glyphosate and HPPD herbicides have also been developed. Furthermore, genetically modified soybeans resistant to three herbicides, glyphosate, glufosinate and 2,4-D, have also been developed under the trade name Enlist E3® Soybean.

Examples of soybeans that have been imparted with insect resistance by genetic engineering techniques include soybeans that have been imparted with resistance to lepidopteran pests, coleopteran pests, dipteran pests, nematodes, and the like.
As an example of soybean that has been rendered resistant to lepidopteran insects by genetic engineering technology, it encodes delta-endotoxin (δ-endotoxin), which is an insecticidal protein derived from soil bacteria Bacillus thuringiensis (hereinafter abbreviated as Bt). Genetically modified soybeans into which the gene to be Examples of delta-endotoxins that confer resistance to lepidopteran insect pests include Cry1A, Cry1Ab, modified Cry1Ab (partially deleted Cry1Ab), Cry1Ac, Cry1Ab-Ac (hybrid protein in which Cry1Ab and Cry1Ac are fused), Cry1C, Cry1F , Cry1Fa2 (modified cry1F), moCry1F (modified Cry1F), Cry1A. 105 (a hybrid protein in which Cry1Ab, Cry1Ac, Cry1F is fused), Cry2Ab2, Cry2Ae, Cry9C, Vip3A, Vip3Aa20 and the like.
Insecticidal proteins that impart insect resistance to soybean include hybrid proteins of the above insecticidal proteins, partially deficient proteins, and modified proteins. Hybrid proteins are produced by the combination of different domains of multiple insecticidal proteins using genetic engineering techniques, such as Cry1Ab-Ac or Cry1A. 105 mag is known. As proteins in which a part has been deleted, Cry1Ab or the like in which a part of the amino acid sequence has been deleted is known. As modified proteins, proteins in which one or more amino acids of natural delta-endotoxin are substituted, such as Cry1Fa2, moCry1F, mCry3A, etc. are known.
In addition, insecticidal proteins derived from Bacillus cereus and Bacillus popilliae (Bacillus popilliae), and insecticidal proteins derived from Bt bacteria Vip1 as insecticidal proteins for imparting insect resistance to soybean by genetic recombination technology , Vip2, Vip3, insecticidal proteins from nematodes, scorpion toxins, spider toxins, spider toxins, toxins produced by animals such as bee toxins or insect specific neurotoxins, filamentous fungal toxins, plant lectins, agglutinin, trypsin inhibitors, serine Protease inhibitors, Protease inhibitors such as patatin, cystatin, papain inhibitors, Lysine, corn-RIP, abrin, luphin, saporin, ribosomal inactivated proteins (RIP) such as briosin, 3-hydroxysteroid oxidase, ecdysteroids -UDP Steroid metabolism enzymes such as glucosyltransferase, cholesterol oxidase, etc., ecdysone inhibitor, HMG-CoA reductase, ion channel inhibitors such as sodium channel, calcium channel inhibitor, juvenile hormone esterase, diuretic hormone receptor, stilbene synthase, bibenzyl synthase , Chitinase, glucanase and the like.

In soybeans whose product quality has been modified by genetic engineering techniques, modification of lignin production, modification of oil or fatty acid components, production of phytic acid degrading enzyme, modification of flower color, modification of alpha-amylase activity, modification of amino acids , Genetically modified soybeans with modified product quality such as modification of starch or carbohydrate component, suppression of acrylamide formation, reduction of black spots due to mechanical damage, anti-allergenicity, reduction of nicotine formation, aging or delayed ripening Be
Genetically modified soybean in which the expression of the same gene is suppressed and oleic acid content is enhanced by introducing a partial gene (gm-fad2-1) of ω-6 desaturase derived from soybean that is a desaturase of fatty acid, Plenish ( It is sold under the trade name of High Oleic Soybean. In addition, a gene for producing a double-stranded RNA of an acyl-acyl carrier protein protein thioesterase gene (fatb1-A) derived from soybean, and a double-stranded RNA of a δ-12 desaturase gene (fad2-1A) derived from soybean A genetically modified soybean whose saturated fatty acid content has been reduced by introducing the resulting gene has been developed under the trade name of Vistive Gold®. Also, a genetically modified soybean has been developed in which the content of ω3 fatty acid is enhanced by introducing the δ-6 desaturase gene (Pj. D6D) derived from primrose and the δ-12 desaturase gene (Nc. Fad3) derived from Neurospora crassa There is.

  Examples of soybeans whose traits related to growth and yield have been modified by genetic recombination techniques include genetically modified soybeans having enhanced growth ability. For example, genetically modified soybeans and the like have been developed in which a gene (bbx32) encoding a transcription factor that controls diurnalities derived from Arabidopsis thaliana has been introduced.

Also, in the above-mentioned soybean, environmental stress resistance, disease resistance, herbicide resistance, pest resistance, as described above, using genetic recombination technology, classical breeding technology, gene marker breeding, genome editing technology etc. Two or more types of property possessed by the parent line by multiplying the lines to which two or more types of growth and yield traits, product quality, fertility traits and the like are given, and genetically modified crops having similar or different properties It also includes the strain given. An example of such a soybean is a genetically modified soybean which has both herbicide resistance and pest resistance.
For example, genetically modified soybeans such as Intacta (registered trademark) Roundup Ready (registered trademark) 2 Pro have been developed as genetically modified soybeans to which glyphosate resistance and pest resistance have been imparted.

Examples of soybeans that have been given disease resistance include varieties that have been provided with soybean rust resistance by conventional breeding methods or genetic engineering techniques. Examples of commonly used resistance genes are, for example, Rpp1, Rpp2, Rpp3, Rpp4, Rpp5, Rpp6. Although this may be inserted into soybean alone, it may be inserted in combination. These genes are described in the following technical documents and the like.
Hyten DL, Hartman GL, Nelson RL, Frederick RD, Concibido VC, Narvel JM and Gregan PB (2007) Map location of the Rpp1 locus that confers resistance to soybean rust in soybean. Crop Science 47: 835-838.
Theoretical and Applied, Silva DCG, Yamanaka N, Brogin RL, Arias CAA, Nepomuceno AL, Di Mauro AO, Pereira SS, Nogueira LM, Passianotto ALL and Abdelnoor RV (2008) Molecular mapping of two loci that confer resistance to Asian rust. Genetics 117: 57-63.
Garcia A, Calvo ES, Kiihl RAS, Harada A, Hiromoto DM and Vieira LGE (2008) Molecular mapping of soybean rust (Phakopsora pachyrhizi) resistance genes: discovery of a novel locus and alleles. Therapeutic and Applied Genetics 117: 545-553.
Chakraborty N, Curley J, Frederick RD, Hyten DL, Nelson RL, Hartman GL and Diers BW (2009) Mapping and confirmation of a new allele at Rpp1 from soybean PI 594538 A conferring RB lesion-type resistance to soybean rust. Crop Science 49: 783-790.
Ray JD, Morel W, Smith JR, Frederik RD and Miles MR (2009) Genetics and mapping of adult plant rust resistance in soybean PI 587886 and PI 587880 A. Theoretical and Applied Genetics 119: 271-280.
Monteros MJ, HA BK, Phillips DV and Boerma HR (2010) SNP assay to detect the 'Hyuuga' red-brown lesion resistance gene for Asian soybean rust. Theoretical Applied Genetics 121: 1023-1032.
Li S, Smith JR, Ray JD and Frederick RD (2012) Identification of a new soybean rust resistance gene in PI 567102 B. Theoretical and Applied Genetics 125: 133-142.
In addition, soybeans that are resistant to soybean diseases other than soybean rust (eg, spot disease, brown ring disease, stem blight etc.), and soybeans that are resistant to multiple diseases including soybean rust Also included.

The present invention controls soybean rust caused by Phakopsora pachyrhizi or Phakopsora meibomiae. The invention is particularly suitable for the control of soybean rust caused by Phakopsora pachyrhizi. The present invention also exerts a control effect on soybean rust caused by Phakopsora pachyrhizi resistant to one or more bactericidal agents selected from the group consisting of QoI agents and DMI agents. F129L type is known to exist in QoI drug resistant bacteria, and F120L type, Y131H / F type, K142R type, I145F type and I475T type are known to exist in DMI drug resistant bacteria . These resistant bacteria are described in the following technical documents and the like.
Klosowski AC, May De Mio LL, Miessner S, Rodrigues R and Stammler G (2016) Detection of the F 129 L mutation in the cytochrome b gene in Phakopsora pachyrhizi.
Pest Manag Sci. Jun; 72 (6): 1211-5.
Schmitz HK1, Medeiros CA, Craig IR and Stammler G (2014) Sensitivity of Phakopsora pachyrhizi towards quinone-outside-inhibitors and demethylation-inhibitors, and corresponding resistance mechanisms.
Pest Manag Sci. Mar; 70 (3): 378-88.

  Hereinafter, the present invention will be described in more detail with formulation examples and test examples, but the present invention is not limited to the following examples. However, in the following examples, parts represent parts by weight unless otherwise specified. Moreover, the application amount (g) of each compound per hectare is represented as g / ha.

Formulation example 1
10 parts of Cartap hydrochloride, 20 parts of Manzeb, 5 parts of polyoxyethylene alkyl ether sulfate, 1 part of phosphoric acid, 2 parts of dextrin, and 62 parts of clay are mixed, water is added and the mixture is kneaded. The kneaded product is granulated with an extrusion granulator equipped with a 0.8 mm diameter screen and then dried to obtain a water dispersible granule.

Formulation example 2
10 parts of Cartap hydrochloride, 20 parts of Manzeb, 5 parts of polyoxyethylene alkyl ether, 1 part of phosphoric acid, 3 parts of powdered urea and 61 parts of clay are mixed, water is added and the mixture is kneaded. The kneaded product is granulated with an extrusion granulator equipped with a 0.8 mm diameter screen and then dried to obtain a water dispersible granule.

Formulation example 3
The same procedure as in Preparation Example 1 is carried out by replacing manzeb with chlorothalonil to obtain a water dispersible granule.

Formulation example 4
27 parts of Cartap hydrochloride, 3 parts of cyproconazole, 5 parts of polyoxyethylene alkyl ether, 1 part of phosphoric acid, 3 parts of powdered urea and 62 parts of clay are mixed, water is added and the mixture is kneaded. The kneaded product is granulated with an extrusion granulator equipped with a 0.8 mm diameter screen and then dried to obtain a water dispersible granule.

Formulation example 5
The same procedure as in Preparation Example 4 is performed by replacing cyproconazole with epoxyconazole to obtain a water dispersible granule.

Formulation Example 6
The same procedure as in Preparation Example 4 is performed by replacing cyproconazole with tebuconazole to obtain a water dispersible granule.

Formulation example 7
The same procedure as in Preparation Example 4 is performed by replacing cyproconazole with prothioconazole to obtain a water dispersible granule.

  Next, test examples are shown.

The products described in the test examples are as follows.
Formulation name Padang (registered trademark) SG aqueous solvent: Granule aqueous solvent formulation containing 75% by weight of cartap hydrochloride NIMBUS (registered trademark): adjuvant containing liquid paraffin (CAS NO. 8012-95-1) as an adjuvant component

Test Example 1
The soil was packed in a plastic pot, soya bean (variety: Kurosengoku) was sown and grown in a greenhouse for 14 days.
First, a spray solution containing cartap hydrochloride was prepared by mixing Padang SG water solvent with a predetermined amount of water and then adding NIMBUS to a predetermined amount. On the other hand, the spray liquid containing the compound A to be tested is prepared by dissolving a predetermined compound A in a mixed solvent of surfactant, dimethylformamide and xylene (surfactant: dimethylformamide: xylene = 1: 10: 8) (Hereinafter referred to as “compound A solution”), a compound A solution was mixed with a predetermined amount of water, and then NIMBUS was added to a predetermined amount to prepare. Further, a spray solution containing cartap hydrochloride and compound A was prepared by mixing a Padang SG water solvent and the compound A solution with a predetermined amount of water, and then adding NIMBUS to a predetermined amount. The addition amount of NIMBUS in this test example was such an amount that the concentration of the adjuvant component in the spray solution was 2,100 mg / L.
The spray solution was applied by foliage treatment so as to adhere to the leaf surface of the soybean. The amount of sprayed liquid was equivalent to 200 liters per hectare. After application, the soybean leaf surface was air-dried, and one day later, it was spray-inoculated with an aqueous suspension (about 10,000 pieces / mL) of summer spores of rust (Phakopsora pachyrhizi). After inoculation, it was placed in a humid atmosphere at 20 to 23 ° C. for 1 day, and then grown in a greenhouse for 13 days (this was treated as a treated area). After that, the lesion area of soybean rust was examined.
From the incidence area rate of the treated area and the incidence area rate of the untreated area, the control value was calculated by the following formula.
Control value (%) = 100 × (1-B / A)
A: Affected area rate of untreated area B: Affected area rate of treated area Here, the untreated area means the same operation as the treated area except that Padang (registered trademark) SG water solvent and / or compound A are not used. It means a ward to do.
The results are shown in Tables 1 to 6.

  According to the present invention, soybean rust can be controlled.

Claims (15)

A method for controlling soybean rust, which comprises applying cartap hydrochloride and a compound selected from the following group (A) to soybeans or a cultivated land of soybeans.
Group (A): Manzeb, chlorothalonil, cyproconazole, epoxiconazole, tebuconazole, and prothioconazole.   The process according to claim 1, comprising the steps of mixing cartap hydrochloride, a compound selected from the group (A) and water to prepare a spray solution, and applying the spray solution to soybeans or soybean cultivation areas. Method for controlling soybean rust.   The method for controlling soybean rust according to claim 2, wherein the step of applying the spray solution is a step of applying to a foliage of soybean.   The weight ratio of cartap hydrochloride to a compound selected from the group (A) is cartap hydrochloride / compound selected from the group (A) = 500/1 to 0.05 / 1. The soybean rust prevention | control method of any one of claim | item 3 characterized by the above-mentioned.   The weight ratio of cartap hydrochloride to a compound selected from the group (A) is cartap hydrochloride / compound selected from the group (A) = 50/1 to 0.1 / 1. The soybean rust prevention | control method of any one of claim | item 3 characterized by the above-mentioned.   The weight ratio of cartap hydrochloride to the compound selected from the group (A) is: cartap hydrochloride / compound selected from the group (A) = 20/1 to 5/1, or 1/1 to 0.2 / 1. The method for controlling soybean rust according to any one of claims 1 to 3, which is 1.   The compound selected from the group (A) is a compound selected from manzeb and chlorothalonil, and the weight ratio of cartap hydrochloride to the compound selected from manzeb and chlorothalonil is a compound selected from cartap hydrochloride / manzeb and chlorothalonil = The method for controlling soybean rust according to any one of claims 1 to 3, wherein the ratio is 1/1 to 0.2 / 1.   The compound selected from the above group (A) is a compound selected from cyproconazole, epoxiconazole, tebuconazole and prothioconazole, and is selected from cartap hydrochloride and cyproconazole, epoxiconazole, tebuconazole and prothioconazole. 4. The compound according to any one of claims 1 to 3, wherein the weight ratio with the compound to be added is: compound selected from cartap hydrochloride / cyproconazole, epoxiconazole, tebuconazole and prothioconazole = 20/1 to 5/1. The method for controlling soybean rust according to item 1.   The soybean rust control according to any one of claims 1 to 8, wherein the total application rate of cartap hydrochloride and the compound selected from the group (A) is 100 to 2,000 g per hectare. Method.   The method for controlling soybean rust according to any one of claims 2 to 9, wherein 1 to 1,000 L of the spray solution is applied per hectare.   The method for controlling soybean rust according to any one of claims 2 to 10, which comprises adding an adjuvant component selected from the group consisting of mineral oil, vegetable oil methyl ester and silicone in the step of preparing a spray solution. .   The method for controlling soybean rust according to claim 11, wherein the concentration of the adjuvant component in the spray solution is 50 to 20,000 mg / L.   13. The method according to any one of claims 1 to 12, wherein cartap hydrochloride and a compound selected from the group (A) are applied in the period from the first double leaf stage (V1 phase) to the mature phase (R8 phase) of soybean. The soybean rust disease control method as described in a term.   The method for controlling soybean rust according to any one of claims 1 to 13, wherein the soybean is soybean to which soybean rust resistance has been imparted.   The method for controlling soybean rust according to any one of claims 1 to 14, wherein the soybean is a genetically modified soybean.