JP2016111938A - Microorganism culture sheet - Google Patents

Abstract

A microorganism culture sheet capable of guaranteeing the sterility of a culture area is provided. A microorganism culture sheet (1) includes a sheet body (10) including a culture region (11) for culturing microorganisms, a cover sheet (20) attached to the sheet body (10) via an attachment region (21), and covering the sheet body (10), Is provided. The cover sheet 20 is held by the holding means 30 so as to cover the culture region 11 of the sheet main body 10. When the state of holding the cover sheet 20 is released, the holding unit 30 cannot hold the cover sheet 20 again. [Selection] Figure 1

Description

  The present invention relates to a microorganism culture sheet for culturing microorganisms, and more particularly to a microorganism culture sheet capable of guaranteeing sterility of a culture region.

  A microorganism culture sheet is known as an instrument for measuring microorganisms in food or the environment. In recent years, the microorganism culture sheet has been widely used as an alternative to the agar medium because it has advantages such as less time for medium adjustment, less space, and reduced disposal costs compared to the conventional agar medium.

  For example, in the microorganism culture sheet shown in Patent Document 1, the base edge of the cover sheet is attached to the sheet body, and the cover sheet covers the culture area of the sheet body. In the microorganism culture sheet shown in Patent Document 1, a test solution containing microorganisms is inoculated into the culture region, and the microorganisms are cultured.

JP 2011-522834 A JP 2001-245893 A

  In order to compensate the reliability of the measurement data of the microorganisms cultured in the culture area, it is necessary to ensure the sterility of the culture area before use. However, in the microorganism culture sheet described in Patent Document 1, since the leading edge of the cover sheet is not fixed to the sheet main body, the cover sheet is rolled up from the culture region when it is carried. For this reason, the culture area was exposed to the atmosphere, and it was difficult to ensure the sterility of the culture area.

  Therefore, as shown in Patent Document 2, a device for preventing the culture region from being exposed by rejoining the front edge of the cover sheet and the sheet main body is disclosed. However, in the microorganism culture sheet shown in Patent Document 2, it is possible to rejoin the cover sheet and the sheet body even after the front edge side of the cover sheet is peeled from the sheet body and the culture region is exposed. is there. For this reason, it cannot be recognized whether the culture area has been exposed to the atmosphere, and the structure is not configured to guarantee the sterility of the culture area.

  This invention is made | formed in view of such a point, and it aims at providing the microorganisms culture sheet which can ensure the sterility of a culture area | region.

  A microorganism culture sheet according to the present invention includes a sheet body including a culture region for culturing microorganisms, a cover sheet attached to the sheet body via an attachment region, and covering the sheet body, and the cover sheet includes a holding unit. Thus, when the state of holding the cover sheet is released, the holding means cannot hold the cover sheet again.

  In the microorganism culture sheet according to the present invention, when viewed from the normal direction to the sheet body, the holding means may hold the cover sheet at a position farther from the attachment region than the culture region. .

  In the microorganism culture sheet according to the present invention, the holding means may hold the cover sheet at a position closer to the attachment region than the culture region when viewed from the normal direction to the sheet body. .

  In the microorganism culture sheet according to the present invention, the holding means may include a joining element that joins the cover sheet to the sheet main body so as to be peelable and non-rejoinable.

  In the microorganism culture sheet according to the present invention, when the cover sheet is peeled from the sheet main body, peeling information may be displayed at a position overlapping with at least one of the joining elements of the cover sheet or the sheet main body. .

  In the microorganism culture sheet according to the present invention, the holding means may include a breakable breaking tape that is stretched between the cover sheet and the sheet body.

  In the microorganism culture sheet according to the present invention, the cover sheet includes a pair of side edges facing in the longitudinal direction of the attachment region, and the holding means is a pair of bonding agents provided along the corresponding side edges. And a pair of separation assisting means formed on the cover sheet so that the portion of the cover sheet bonded to each bonding agent can be separated from the other portions.

  According to the present invention, once the state in which the holding means holds the cover sheet is released, the cover sheet cannot be held again. For this reason, as long as the holding means maintains the state of holding the cover sheet, it is guaranteed that the state in which the cover sheet protects the culture region of the sheet body is maintained. Therefore, by maintaining the state in which the holding means holds the cover sheet until the microorganism culture sheet is used, the sterility of the culture region can be guaranteed.

It is a top view which shows the microorganisms culture sheet by the 1st Embodiment of this invention. It is sectional drawing of the microorganisms culture sheet along line II-II shown in FIG. It is sectional drawing of the microorganisms culture sheet | seat along line IIIII-III shown in FIG. FIG. 2 is a perspective view showing a state in which the cover sheet is lifted from the sheet main body in the microorganism culture sheet shown in FIG. 1. In the microorganism culture sheet shown in FIG. 1, it is a top view which shows the modification of a holding means. In the microorganism culture sheet shown in FIG. 1, it is a top view which shows another modification of a holding means. FIG. 7 is a perspective view showing a state in which the cover sheet is rolled up in the microorganism culture sheet shown in FIG. 6. In the microorganism culture sheet shown in FIG. 1, it is a top view which shows another modification of a holding means. In the microorganism culture sheet shown in FIG. 1, it is a top view which shows another modification of a holding means. In the microorganism culture sheet shown in FIG. 1, it is a top view which shows another modification of a holding means. It is a figure corresponding to FIG. 3, Comprising: It is sectional drawing of the microorganism culture sheet | seat shown in FIG. FIG. 11 is a perspective view showing a state in which the cover sheet is lifted from the sheet body in the microorganism culture sheet shown in FIG. 10. It is a top view which shows the microorganisms culture sheet by the 2nd Embodiment of this invention. It is a side view of the microorganism culture sheet shown in FIG. It is a perspective view which shows the state which raised the cover sheet in the microorganism culture sheet | seat shown in FIG.

  Embodiments of the present invention will be described below with reference to the drawings. In the drawings attached to the present specification, for the sake of illustration and ease of understanding, the scale, the vertical / horizontal dimension ratio, and the like are appropriately changed and exaggerated from those of the actual product.

<< First Embodiment >>
FIG. 1 is a plan view showing a microorganism culture sheet 1 according to the first embodiment of the present invention, and FIG. 2 is a cross-sectional view of the microorganism culture sheet 1 taken along line II-II shown in FIG.

  The microorganism culture sheet 1 is used for measuring microorganisms in food or the environment, for example. As shown in FIGS. 1 and 2, a microorganism culture sheet 1 includes a sheet-shaped sheet body 10 including a culture region 11 for culturing microorganisms and a non-culture region 12 outside the culture region 11, A sheet-like cover sheet 20 that covers the sheet main body 10 is provided.

  As shown in FIG. 1, when viewed from the normal direction nd (see FIG. 2) to the sheet-like sheet main body 10, the sheet main body 10 and the cover sheet 20 have a rectangular shape. In the present specification, the “normal direction nd to the sheet main body 10” means the normal direction nd to the surface that coincides with the plane direction of the sheet main body 10 when the sheet main body 10 is viewed as a whole and globally. Say. In the present embodiment, the normal direction nd to the sheet body 10 coincides with the normal direction to the back surface 14 of the sheet body 10 described later.

  Specifically, the sheet body 10 includes a straight base end edge 10a, a straight front end edge 10d facing the base end edge 10a, and the end portions of the base end edge 10a and the front end edge 10d in plan view. And includes a linear side edge 10b and a side edge 10c facing each other. Similarly, the cover sheet 20 extends between the straight base end edge 20a, the straight front end edge 20d facing the base end edge 20a, and the ends of the base end edge 20a and the front end edge 20d in plan view. The linear side edge 20b and the side edge 20c which oppose each other are included.

  In the present embodiment, the base end edge 20a and the pair of side edges 20b and 20c of the cover sheet 20 overlap with the base end edge 10a and the pair of side edges 10b and 10c of the corresponding sheet body 10 in plan view. . Further, the front end edge 20d of the cover sheet 20 is shifted from the front end edge 10d of the sheet body 10 in plan view. Specifically, the leading edge 20d of the cover sheet 20 protrudes from the leading edge 10d of the sheet body 10 and protrudes outward from the leading edge 10d. As a result, the vicinity of the tip edge 20d of the cover sheet 20 can be easily grasped, and the cover sheet 20 can be easily lifted from the sheet main body 10.

  As shown in FIG. 2, the sheet main body 10 has a front surface 13 facing the cover sheet 20 and a back surface 14 facing the opposite side of the cover sheet 20. On the surface 13 of the sheet body 10, the culture region 11 and the non-culture region 12 outside the culture region 11 are arranged.

  On the other hand, the cover sheet 20 has an inner surface 23 facing the sheet main body 10 and an outer surface 24 facing the opposite side of the sheet main body 10.

  As shown in FIG. 1, the cover sheet 20 is attached to the sheet main body 10 via an attachment region 21 provided along the base end edge 20 a. Since the cover sheet 20 is attached to the sheet main body 10 via the attachment region 21, the cover sheet 20 can be easily lifted with respect to the sheet main body 10 with the attachment region 21 as a starting point.

  In the illustrated example, the attachment region 21 is formed as a belt-like region extending between the pair of side edges 10b and 10c along the base edge 20a in plan view. Therefore, the longitudinal direction d1 of the attachment region 21 coincides with the opposing direction of the pair of side edges 10b and 10c. Typically, an adhesive is applied to the attachment region 21. The attachment region 21 of the cover sheet 20 and the non-culture region 12 of the sheet body 10 are joined via this adhesive.

  Further, as shown in FIG. 1, the sheet body 10 includes a frame 15 that surrounds the culture region 11 and partitions the culture region 11 and the non-culture region 12. The frame 15 can prevent the test solution containing the microorganisms supplied to the culture region 11 from spreading beyond the frame 15. In the example illustrated in FIG. 1, an example in which the frame body 15 has a circular contour in plan view is illustrated. However, the outline of the frame 15 is not particularly limited, and may be a square, an ellipse, a polygon, an indefinite shape, or the like.

  Here, the culture region 11 refers to a region for culturing microorganisms. In the present embodiment, the culture region 11 is composed of a dry medium containing at least a nutrient component as described later. A dry culture medium is obtained by drying a medium solution containing a solvent to volatilize the solvent. The culture region 11 can be inoculated with a test solution containing microorganisms to culture the microorganisms.

  The culture region 11 further includes, for example, a fixing agent, a plasticizer, a gelling agent, and a nonwoven fabric in addition to the above-described nutrient components. Furthermore, the culture region 11 may contain a color development indicator, a selection agent, and the like. Each component of the culture region 11 is appropriately selected according to the microorganism to be cultured. The culture region 11 may be composed of a single layer or may be composed of two or more layers.

  On the other hand, the non-culture area 12 refers to an area other than the culture area 11 in the sheet body 10. In the non-culture area 12, for example, an ID number, an expiration date, a trademark, a one-dimensional code, and a two-dimensional code are printed.

  By the way, in order to compensate the reliability of the measurement data of the microorganisms cultured in the culture region 11, it is necessary to maintain the sterility of the culture region 11 before use. Therefore, the microorganism culture sheet 1 of the present embodiment has a holding means 30 that holds the cover sheet 20 so as to cover the culture region 11 of the sheet body 10.

  The holding means 30 of the present embodiment includes a joining element 31 that joins the surface 13 of the sheet body 10 and the inner surface 23 of the cover sheet 20 in a peelable manner between the sheet body 10 and the cover sheet 20.

  In the plan view shown in FIG. 1, the joining element 31 is provided at a position farther from the attachment region 21 than the culture region 11. That is, the joining element 31 holds the cover sheet 20 at a position farther from the attachment region 21 than the culture region 11. The joining element 31 shown in FIG. 1 is located in the vicinity of a corner region defined by the side edge 10b and the tip edge 10d. However, it is not limited to such an example, and the joining element 31 may be located not in the vicinity of the corner region but in the vicinity of the central region between the culture region 11 and the tip edge 10d.

  The joining element 31 of the present embodiment is configured such that when the cover sheet 20 is peeled from the sheet main body 10, the cover sheet 20 cannot be joined to the sheet main body 10 again. In other words, the joining element 31 cannot hold the cover sheet 20 again when the state of holding the cover sheet 20 is released.

  FIG. 3 shows a layer structure of the joining element 31. As shown in FIG. 3, the joining element 31 includes a first adhesive layer 31a, a release layer 31b, a base layer 31c, and a second adhesive layer 31d, which are arranged from the cover sheet 20 side toward the sheet body 10 side. Includes in order.

  The base layer 31 c is provided to hold each layer constituting the joining element 31. Although it does not specifically limit as a material which makes the base layer 31c, For example, polyester resins, such as a polyethylene terephthalate, are mentioned.

  The 1st adhesion layer 31a is arrange | positioned between the cover sheet 20 and the base layer 31c, and the 1st adhesion layer 31a joins the cover sheet 20 and the base layer 31c. Moreover, the 2nd adhesion layer 31d is arrange | positioned between the sheet | seat main body 10 and the base layer 31c, and the 2nd adhesion layer 31d joins the sheet | seat main body 10 and the base layer 31c. As the adhesive layers 31a and 31d, for example, an acrylic adhesive or a rubber adhesive can be used.

  A release layer 31b is partially disposed on the base layer 31c. That is, the release layer 31b is arranged in a pattern on the base layer 31c. The release layer 31b exhibits non-adhesiveness with respect to the first adhesive layer 31a. Since the release layer 31b exhibiting non-adhesiveness is partially disposed on the base layer 31c, the bonding force between the first adhesive layer 31a and the base layer 31c can be weakened, and the first adhesive layer 31a and the base layer 31c can be weakened. Provides peelability to the joint. As an example, as a material forming the release layer 31b, for example, a silicone release agent or a fluorine release agent can be used.

  Further, as shown in FIG. 3, the cover print layer 26 is printed at a position overlapping the joining element 31 of the cover sheet 20, and the main body printing layer 16 is printed at a position overlapping the joining element 31 of the sheet body 10. ing. On each of the print layers 16 and 26, peeling information that informs that the cover sheet 20 has been peeled from the sheet body 10 is printed. The peeling information may be any information that can visually recognize that the cover sheet 20 has been peeled from the sheet main body 10, and examples thereof include a pattern, a pattern, and characters. In the present embodiment, when the cover sheet 20 is peeled from the sheet main body 10, characters “open” are displayed on the sheet main body 10 and the cover sheet 20 (see FIG. 4). In the state shown in FIG. 1 in which the cover sheet 20 is bonded to the sheet main body 10, the cover sheet 20 faces the outer surface 24 side of the cover print layer 26 in order to conceal the characters “open” provided on the print layers 16 and 26. The surface is colored dark.

  Next, the operation of the microorganism culture sheet 1 of the present embodiment having the above configuration will be described with reference to FIG. FIG. 4 is a perspective view illustrating a state in which the cover sheet 20 is lifted from the sheet main body 10.

  First, as shown in FIG. 1, in a storage state before using the microorganism culture sheet 1, the cover sheet 20 is joined to the sheet body 10 via the joining element 31 to cover the culture region 11 of the sheet body 10. Yes.

  As shown in FIG. 4, when the cover sheet 20 is peeled from the sheet body 10 and rolled up, the culture region 11 is exposed. At this time, peeling information “open” is displayed on the main body printing layer 16 printed on the sheet body 10, and peeling information “open” is also displayed on the cover printing layer 26 printed on the cover sheet 20.

  Subsequently, the exposed culture region 11 is inoculated with a test solution containing microorganisms. Thereafter, the culture region 11 is covered again with the cover sheet 20. However, the joining element 31 cannot join the cover sheet 20 to the sheet body 10 again when the cover sheet 20 is peeled from the sheet body 10. Therefore, even if the culture region 11 is covered again with the cover sheet 20, the cover sheet 20 and the sheet body 10 are not joined again via the joining element 31.

  Thereafter, the microorganism culture sheet 1 is stored in an incubator, and the microorganisms inoculated in the culture region 11 are cultured.

  As described above, according to the present embodiment, the sheet body 10 including the culture region 11 for culturing microorganisms, and the cover sheet attached to the sheet body 10 via the attachment region 21 and covering the sheet body 10 20, and the cover sheet 20 is held by the holding means 30 so as to cover the culture region 11 of the sheet main body 10. When the holding means 30 is released from the state of holding the cover sheet 20, The cover sheet 20 cannot be held again. That is, when the state of holding the cover sheet 20 is released, the holding unit 30 is maintained in the state of releasing the cover sheet 20. According to such a form, as long as the holding means 30 maintains the state in which the cover sheet 20 is held, it is guaranteed that the state in which the cover sheet 20 protects the culture region 11 of the sheet body 10 is maintained. The Therefore, it is possible to guarantee the sterility of the culture region 11 by maintaining the state in which the holding means 30 holds the cover sheet 20 until the microorganism culture sheet 1 is used.

  Further, according to the present embodiment, the holding means 30 holds the cover sheet 20 at a position farther from the attachment region 21 than the culture region 11 in plan view. In this case, since the cover sheet 20 can be fixed to the sheet body 10 at a position beyond the culture region 11, the culture region 11 can be covered with the cover sheet 20 without any gap. As a result, it is possible to guarantee the sterility of the culture region 11 with high reliability.

  Further, according to the present embodiment, the holding means 30 includes the joining element 31 that joins the cover sheet 20 to the sheet main body 10 in a peelable and non-rejoinable manner. In this case, the holding means 30 can be realized in a simple manner.

  Further, according to the present embodiment, when the cover sheet 20 is peeled from the sheet main body 10, the peeling information 16 and 26 is located at a position overlapping with at least one joining element 31 of the cover sheet 20 or the sheet main body 10. Is displayed. In this case, the user can more reliably recognize that the cover sheet 20 has been peeled from the sheet body 10.

≪Modification≫
Note that various modifications can be made to the above-described embodiment. Hereinafter, an example of modification will be described with reference to the drawings. In the following description and the drawings used in the following description, the same reference numerals as those used for the corresponding parts in the above embodiment are used for the parts that can be configured in the same manner as in the above embodiment. A duplicate description is omitted.

  In the above-described embodiment, as shown in FIG. 1, the example in which the holding unit 30 includes the single joining element 31 has been described. However, the configuration of the joining element 31 is not limited to the above-described example. 5 to 10 show other configuration examples of the joining element 31. FIG. Among these, in the example shown in FIG. 5, the plurality of joining elements 31 are provided at positions farther from the attachment region 21 than the culture region 11 in plan view. In particular, in plan view, one joining element 31 is disposed in the vicinity of a corner region defined by one side edge 10b and the tip edge 10d, and the other joining element 31 is disposed with the other side edge 10c. It is located near another corner area defined by the tip edge 10d. In this case, since the culture region 11 of the sheet body 10 can be covered with the cover sheet 20 without any gap, the sterility of the culture region 11 can be ensured with high reliability.

  In the example shown in FIG. 6, in the plan view, one joining element 31 is provided at a position farther from the attachment region 21 than the culture region 11, and the other joining element 31 is more attachment region than the culture region 11. 21 is provided at a position close to 21. In particular, in a plan view, one joining element 31 is located in the vicinity of a corner region defined by one side edge 10b and the leading edge 10d of the sheet body 10, and the other joining element 31 is a cover sheet. 20 is located in the vicinity of one side edge 20 c and the attachment region 21.

  FIG. 7 shows a state in which the cover sheet 20 is lifted from the sheet body 10 in the microorganism culture sheet 1 shown in FIG. As shown in FIG. 7, when the cover sheet 20 is peeled off from the sheet main body 10 and rolled up, the peeling information 16 and 26 “open” is located at the position where the cover sheet 20 and each joining element 31 of the sheet main body 10 overlap. Is displayed.

  The microorganism culture sheet 1 shown in FIGS. 6 and 7 is particularly advantageous in environmental inspection. That is, in the environmental inspection, the cover sheet 20 is lifted from the sheet body 10 and sterilized water is first injected into the exposed culture region 11. At this time, the joining element 31 near the leading edge 10d is released from the joined state in which the sheet body 10 and the cover sheet 20 are joined, and cannot be rejoined. On the other hand, the joining element 31 close to the base edge 10a is maintained in a joined state in which the sheet body 10 and the cover sheet 20 are joined. Therefore, when the joining element 31 near the distal end edge 10d is released from the joining state and the joining element 31 near the proximal end edge 10a is maintained in the joining state, it can be determined that the step of injecting sterilized water has been performed. .

  Next, the culture region 11 of the sheet body 10 is covered again with the cover sheet 20. As a result, the sterilized water injected into the culture region 11 can be expanded without causing unevenness in the culture region 11, and the culture region 11 can be gelled without unevenness.

  Subsequently, as shown in FIG. 7, the cover sheet 20 is lifted from the sheet main body 10 to expose the culture region 11. This state is maintained for about 15 minutes, for example, and the bacteria in the environment are taken into the culture region 11. At this time, the joining element 31 near the base end edge 10a is also released from the joined state and cannot be rejoined. Therefore, when both the joining element 31 near the distal end edge 10d and the joining element 31 near the proximal end edge 10a are released from the joined state, it can be determined that the bacteria uptake step has also been performed.

  Thereafter, the culture region 11 of the sheet body 10 is covered again with the cover sheet 20. Then, the microorganism culture sheet 1 is stored in an incubator, and the microorganisms inoculated in the culture region 11 are cultured.

  As described above, according to the microorganism culture sheet 1 shown in FIGS. 6 and 7, from the combination of the joining state and the releasing state of the joining element 31 near the leading edge 10 d and the joining element 31 near the base edge 10 a, You can also grasp the progress. In addition, it is possible to determine whether the microorganism culture sheet 1 has been used, using the released state of the joining element 31 near the tip edge 10d as a mark. As a result, it contributes to easy environmental inspection by the microorganism culture sheet 1.

  Next, in the example illustrated in FIG. 8, the single joining element 31 is provided at a position farther from the attachment region 21 than the culture region 11 in plan view. The joining element 31 shown in FIG. 8 is formed as a band-like region extending between the pair of side edges 10b and 10c along the tip edge 20d.

  Subsequently, in the example illustrated in FIG. 9, the holding unit 30 includes a pair of bonding agents 32 provided along the side edges 20 b and 20 c of the cover sheet 20 corresponding to each, and each bonding agent in the cover sheet 20. A pair of auxiliary assisting means 33 formed on the cover sheet 20 is included so that the part joined to 32 can be separated from other parts.

  In the plan view shown in FIG. 9, each bonding agent 32 extends in a band shape from a position where the attachment region 21 of the cover sheet 20 is provided to a position where the leading edge 10 d of the sheet body 10 is provided. On the other hand, each separation assisting means 33 extends in parallel with the side edge 20c in a region between the bonding agent 32 and the culture region 11 in plan view. Each bonding agent 32 shown in FIG. 9 is linearly formed from the base end edge 20a to the front end edge 20d of the cover sheet 20 in plan view.

  As an example, the separation assisting means 33 may be a perforation formed by a cut formed intermittently in the cover sheet 20 or a half cut line formed by a groove formed continuously in the cover sheet 20.

  On the other hand, in the example shown in FIG. 10, the joining element 31 is provided in the entire region of the non-culture region 12 of the sheet body 10 other than the portion overlapping the attachment region 21. The joining element 31 joins the cover sheet 20 to the sheet main body 10 in a peelable and non-rejoinable manner. The joining element 31 of the present embodiment is made of a so-called pressure-sensitive joining agent.

  In FIG. 11, the cross section of the microorganism culture sheet 1 containing the joining element 31 is shown. The joining element 31 shown in FIG. 11 is configured by a single layer. As an example of such a pressure-sensitive type bonding agent, natural rubber-based bonding agents represented by raw natural rubber latex and raw natural rubber latex obtained by graft copolymerization with methyl methacrylate (MMA) can be mentioned.

  FIG. 12 shows a state in which the cover sheet 20 is lifted from the sheet body 10 in the microorganism culture sheet 1 shown in FIG. As shown in FIG. 12, the joining element 31 cannot join the cover sheet 20 to the sheet body 10 again after the cover sheet 20 is peeled from the sheet body 10.

  According to the joining element 31 shown in FIGS. 10 to 12, a wide range of the sheet body 10 and the cover sheet 20 can be joined in a peelable and rejoinable manner. For this reason, the culture region 11 can be covered with the cover sheet 20 without any gap, and the sterility of the culture region 11 can be ensured with high reliability.

<< Second Embodiment >>
Next, a second embodiment of the present invention will be described with reference to FIGS. 13 and 14 are a plan view and a cross-sectional view, respectively, showing the microorganism culture sheet 1 in the second embodiment of the present invention. FIG. 15 is a perspective view illustrating a state in which the cover sheet 20 is lifted from the sheet main body 10. The second embodiment described with reference to FIGS. 13 to 15 differs in the configuration of the holding means 30, but the other configurations can be configured in the same manner as the first embodiment and its modifications. . In the following description of the second embodiment and the drawings used in the following description, the parts that can be configured in the same manner as in the first embodiment and the modifications thereof are the correspondence in the first embodiment described above. The same reference numerals as those used for the parts to be used are used, and redundant description is omitted.

  As shown in FIG. 13 and FIG. 14, the holding means 30 of the present embodiment includes a plurality of breakable tapes 34 that are stretched between the sheet body 10 and the cover sheet 20. Each breaking tape 34 extends from the back surface 14 of the sheet body 10 to the outer surface 24 of the cover sheet 20 through the sheet body 10 and the side edges 10c and 20c of the cover sheet 20.

  In the plan view shown in FIG. 13, one breaking tape 34 is provided at a position farther from the attachment area 21 than the culture area 11, and the other breaking tape 34 is a position closer to the attachment area 21 than the culture area 11. Is provided. In the plan view shown in FIG. 13, one breaking tape 34 is located in the vicinity of a corner region defined by the side edge 10 c and the leading edge 10 d of the sheet body 10, and the other breaking tape 34 is formed on the cover sheet 20. It is located in the area | region used as the vicinity of the attachment area | region 21 and the side edge 10c. However, it is not limited to such an example, and the joining element 31 may be located not in the vicinity of the corner region but in the vicinity of the central region between the culture region 11 and the tip edge 10d.

  FIG. 15 shows a state where the cover sheet 20 is lifted from the sheet body 10. As shown in FIG. 15, when the cover sheet 20 is lifted from the sheet main body 10, each breaking tape 34 is broken. Therefore, when the cover sheet 20 is lifted from the sheet main body 10, the breaking tape 34 cannot re-join the cover sheet 20 to the sheet main body 10. In other words, the breakable tape 34 cannot hold the cover sheet 20 again when the state holding the cover sheet 20 is released.

  As described above, according to the present embodiment, the sheet body 10 including the culture region 11 for culturing microorganisms, and the cover sheet attached to the sheet body 10 via the attachment region 21 and covering the sheet body 10 20, and the cover sheet 20 is held by the holding means 30 so as to cover the culture region 11 of the sheet main body 10. When the holding means 30 is released from the state of holding the cover sheet 20, The cover sheet 20 cannot be held again. According to such a form, as long as the holding means 30 maintains the state in which the cover sheet 20 is held, it is guaranteed that the state in which the cover sheet 20 protects the culture region 11 of the sheet body 10 is maintained. The Therefore, it is possible to guarantee the sterility of the culture region 11 by maintaining the state in which the holding means 30 holds the cover sheet 20 until the microorganism culture sheet 1 is used.

  Further, according to the present embodiment, the holding means 30 holds the cover sheet 20 at a position farther from the attachment region 21 than the culture region 11 in plan view, and the attachment region 21 than the culture region 11. The cover sheet 20 is held even at a position close to. According to such a form, as described above, it is possible to easily grasp the progress status of the environmental inspection, which contributes to easily performing the measurement work using the microorganism culture sheet 1.

  Further, according to the present embodiment, the holding means 30 includes the breakable breaking tape 34 that is stretched between the cover sheet 20 and the sheet main body 10. In this case, when the cover sheet 20 is lifted from the sheet body 10, the broken tape 34 remains on the sheet body 10 and the cover sheet 20. For this reason, it becomes possible for a user to recognize more reliably that the cover sheet 20 peeled from the sheet | seat main body 10 by confirming the fracture | rupture tape 34 broken.

DESCRIPTION OF SYMBOLS 1 Microbe culture sheet 10 Sheet | seat main body 10a Base edge 10b, 10c Side edge 10d Tip edge 11 Culture area | region 16 Peeling information (main body printing layer)
20 Cover sheet 20a Base edge 20b, 20c Side edge 20d Tip edge 21 Attachment area 26 Peeling information (cover printing layer)
30 Holding means 31 Joining element 32 Joining agent 33 Separation assisting means 34 Breaking tape

Claims (7)

A sheet body including a culture region for culturing microorganisms;
A cover sheet that is attached to the seat body via an attachment area and covers the seat body;
The cover sheet is held by a holding means so as to cover the culture region of the sheet body,
The microorganism culture sheet, wherein the holding unit cannot hold the cover sheet again when the state of holding the cover sheet is released.   The microorganism culture sheet according to claim 1, wherein the holding means holds the cover sheet at a position farther from the attachment area than the culture area when viewed from the normal direction to the sheet body. .   The microorganism culture sheet according to claim 2, wherein the holding means holds the cover sheet at a position closer to the attachment area than the culture area when viewed from the normal direction to the sheet body. .   4. The microorganism culture sheet according to claim 1, wherein the holding unit includes a joining element that joins the cover sheet to the sheet main body in a peelable and non-rejoinable manner. 5.   The microorganism culture sheet according to claim 3, wherein when the cover sheet is peeled from the sheet main body, peel information is displayed at a position overlapping with the joining element of at least one of the cover sheet or the sheet main body. .   The microorganism culture sheet according to any one of claims 1 to 5, wherein the holding means includes a breakable breaking tape that is stretched between the cover sheet and the sheet main body. The cover sheet includes a pair of side edges facing in the longitudinal direction of the attachment region,
The holding means includes a pair of bonding agents provided along side edges corresponding to each of the holding means, and a portion of the cover sheet bonded to each bonding agent is separable from other portions. The microorganism culture sheet according to claim 1, comprising a pair of separation assisting means formed on the cover sheet.

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