JP2016013991A - Use of Kampo herbal compounds and extracts for anti-uterine fibroids - Google Patents

Abstract

[PROBLEMS] To prevent and improve symptoms of uterine fibroids. A herbal compound for anti-uterine fibroids is composed of 25% to 75% gyudon, 20.8% to 62.5% jaundice and 4.2% to 12.5% beneficial mother grass by mass percentage. including. The extract of herbal medicine is obtained by extracting the above-mentioned herbal medicine herb composition with 95% alcohol at 50 to 80 ° C. in 8 hours to 12 hours, and concentrating the extracted product. Obtaining an extract of [Selection] Figure 3

Description

  The present invention relates to a herbal medicine composition, and more particularly, to a herbal medicine composition for anti-uterine fibroids. Furthermore, the present invention relates to a herbal medicine composition used for producing a use of an anti-uterine fibroid drug. It is related to the extract.

  Uterine fibroid is a benign tumor that originates from the myometrium, occurs frequently in women aged 30 to 50 years, and most uterine fibroids have no obvious symptoms. . However, as hysteromyoma grows, not only heavy painful periods and abnormal gynecologic hemorrhage may occur, but in severe cases, infertility may occur. At this time, it will be removed by uterine myomectomy surgery.

  As a conventional drug used to improve the disease of uterine fibroids, for example, a non-steroidal anti-inflammatory drug (hereinafter referred to as NSAID) is used to treat pain generated from uterine fibroids. Or use oral contraceptive pills (hereinafter referred to as OCP) to reduce bleeding phenomena. In addition, gonadotropin-releasing hormone analog (hereinafter referred to as GnRH analog) is taken to suppress the content of estrogen in the body, thereby suppressing the growth of uterine fibroids. can do.

  However, long-term use of the above GnRH analogs may result in symptoms such as osteoporosis or postmenopausal symptom, and after stopping medication, uterine fibroids may continue to grow Therefore, it is recommended to use only specific patients. The conventional drug described above is, for example, disclosed in US Publication No. 2006/0251581 (Patent Document 1).

  Accordingly, it is still necessary to provide a herbal medicine composition to cope with the disease of uterine fibroids.

US Publication No. 2006/0251581

  The present invention has been invented in view of such problems, and an object of the present invention is to provide a herbal medicine composition for anti-uterine fibroids that can suppress the growth of uterine fibroids. There is.

  The second object of the present invention is to extract a herbal medicinal compound used for an anti-uterine fibroid drug that can improve the pathology of uterine fibroids and improve the quality of life of patients with the active ingredients extracted from the above-mentioned herbal medicinal compound. To provide things.

  In order to achieve the above object, the herbal medicine composition for anti-uterine fibroids according to the present invention comprises 25% to 75% gyudon, 20.8% to 62.5% jaundice and 4.2% by mass percentage. Contains ~ 12.5% beneficial mother grass.

  Further, the herbal medicine composition for anti-uterine fibroids according to the present invention may contain 75% of cowpox in mass percentage in the above-mentioned herbal medicine composition. In addition, the Chinese herbal medicine composition may contain 20.8% jaundice by mass percentage. In addition, 4.2% by mass of beneficial herb can be included in the above-mentioned herbal medicine composition. The herbal medicinal herb composition may also contain 75% beef meal, 20.8% jaundice, and 4.2% beneficial herb by mass percentage.

  In addition, the herbal medicinal herb extract used for producing the use of the anti-uterine fibroid drug according to the present invention, the herbal medicinal herb extract is produced by a method including the following steps, and has any of the above compositions A step of providing a herbal medicine composition, a step of extracting the above-mentioned herb medicine composition in 8 hours to 12 hours by 95% alcohol at 50 ° C. to 80 ° C., and concentrating the extracted product, Obtaining a herbal medicine herb extract, wherein the weight-volume ratio of the herbal medicine compound to 95% sake is 1: 2, the beef bowl is a fruit body, the jaundice is the root, and the beneficial mother grass Is a ground part.

  In addition, an extract of a herbal medicinal herb used to manufacture the use of the anti-uterine fibroid drug according to the present invention is obtained by administering the above-mentioned herbal medicinal herb extract to a single consumer, so The amount of the herbal medicine extract administered to the consumer is 300 milligrams of the herbal extract per kilogram of the consumer's body weight every day, and for 28 consecutive days. Can also be administered.

  According to the present invention, the herbal medicine composition for anti-uterine fibroids can suppress the content of estrogen and lutein hormone in the body of the consumer due to the compositional distribution of cowpox, jaundice, and beneficial mother grass. There is an advantage that the effect of suppressing the growth can be achieved.

  According to the present invention, an extract of a herbal medicine used for producing an anti-uterine fibroid drug application extracts an active ingredient from cowpox, jaundice, and beneficial herb, and effectively suppresses the growth of uterine fibroids. Thus, there is an advantage that the patient can be exempted from diseases such as menstrual pain and abnormal bleeding due to the growth of uterine fibroids, and the effect of improving the patient's quality of life can be achieved.

FIG. 1 shows the results of H & E staining of a uterine section of the C0 group Captain. FIG. 2 is a result of H & E staining of a uterine horn section of the C0 group Captain. FIG. 3 shows the results of H & E staining of the uterine section of the first C1 set. FIG. 4 shows the results of H & E staining of a uterine horn section of the first C1 set.

  Embodiments of the present invention will be described below with reference to the drawings.

  The herbal medicine composition of the present invention includes Antrodia cinnamomea, Astragali radix, and Leonurus japonicus. According to the compositional distribution of cowpox, jaundice and beneficial mother grass, Activity can be suppressed.

  In more detail, the herbal medicine composition comprises 25% to 75% beef meal, 20.8% to 62.5% jaundice, and 4.2% to 12.5% beneficial mother grass by mass percentage, The herbal medicinal herb composition preferably further comprises 75% gyudon, 20.8% jaundice and 4.2% beneficial mother grass by mass percentage. Thereby, the above-mentioned herbal medicine herb compound suppresses the growth of uterine fibroids through the cooperative action of various active ingredients, and can exempt diseases such as menstrual pain and abnormal bleeding caused by uterine fibroid patients.

In addition, beef bowls are selected from dish-cultured cow pod bodies and contain many terpenes. Jasmine selectively consists of the dry roots of the moss family Astragalus membranaceus (Fisch.) Bge. Var. Mongholicus (Bge.) Hsiao or Hymenoptera astragalus membranaceus (Fisch.) Bge. Contains abundant glycosides, polysaccharides, flavone compounds, amino acids and trace elements. Beneficial herb selectively consists of a dry above-ground part of the plant and contains various alkaloids such as leonurine (Leonurine, C ₁₄ H ₂₁ N ₃ O ₅ ), leonuridine (Leonuridine, C ₁₅ H ₂₄ O ₉ ), stachydrin, Furthermore, benzoic acid, potassium chloride, lauric acid, linolenic acid, oleic acid, vitamin A and flavone substances are contained.

  Since the herbal medicine composition of the present invention can effectively suppress the growth of uterine fibroids, it can be a kind of active ingredient that assists in the suppression of uterine fibroids, preferably extracted from the above-mentioned herbal medicine composition. The herbal extract obtained in this way can be applied to the manufacture of a drug or health food for preventing or treating uterine fibroids. The above-mentioned extract of herbal medicine can be formed as a single pharmaceutical composition by combining with a carrier or excipient that is acceptable in pharmacology. In addition, the above-described extract of herbal medicine can be produced in any edible form. For example, as a edible form by combining a tablet, a stool model, a tablet, a capsule, a powder, a granule, a liquid, or the like, or the above-mentioned herbal extract with other foods or beverages. It can be provided to be taken orally by an organism.

  In addition, the above-mentioned extract of herbal medicine is preferably produced by a method including the following steps. Providing the above-described herbal medicine composition, extracting the above-mentioned herbal medicine composition with 95% alcohol at 50 ° C to 80 ° C in 8 hours to 12 hours, and concentrating the extracted product. And obtaining an extract of the herbal medicine.

  More specifically, the above-mentioned herbal medicine composition is mixed with the above 95% spirits in a weight / volume ratio of 1: 2, so that various active ingredients contained in the above-mentioned herbal medicine composition are 95% above. It can elute into spirits.

  In this example, 500 grams of Chinese herbal herb compound was extracted with 1 liter of 95% alcohol, refluxed at a constant temperature of 60 ° C. for 10 hours, repeated extraction three times, and finally vacuum filtration. The extract of herbal medicine is obtained by vacuum concentration and freeze-drying.

  In addition, the herbal medicine extract can be administered to one consumer, for example, 300 milligrams of the herbal extract per kilogram of the consumer's body weight is administered daily and administered continuously for 28 days. Thus, since various active ingredients can act in the body of the consumer, growth of uterine fibroids can be suppressed.

  In order to prove that the herbal medicine composition of the present invention has anti-uterine fibroid activity, the following test was conducted.

(A) Manufacture of Kampo herb extract and detection of active ingredient content In this test, the composition ratio of each set of Kampo herb compound is as shown in Table 1, and the method for producing the above-mentioned Kampo herb extract Is as described above, each 500 grams of each herb herb compound is extracted with 1 liter of 95% alcohol, filtered, concentrated and freeze-dried, then immediately extracted each group of herb herb I won a thing.

  Weigh each 0.2 gram of each herbal herb extract into a spiral test tube, add 5 mL of methanol, vibrate with ultrasound for 15 minutes, then centrifuge at 3000 rpm for 10 minutes. Then, take 5 mL of the supernatant and place it in a clean test tube, then bath at 100 ° C. and heat to dryness.

  Subsequently, by performing selective analysis using a tube column of Purospher STAR (Merck) RP-18e (5 μm) 250 mm × 4 mm, acetonitrile (Acetonitrile, hereinafter referred to as ACN) at a volume ratio of 47:53 is obtained. Table 2 shows the results of analyzing the percentage of the total area occupied by each set of triterpenoids at a flow rate of 1 mL / min, a detection wavelength of 254 nm, and a 0.085% phosphoric acid solution as a mobile phase. Show.

  Furthermore, using galactose as a standard product, a standard curve is produced using the concentration and absorbance. Subsequently, each group of herbal medicinal herb extract solution was placed, 1 mL of each group of herbal medicinal herb extract was taken and added to 1 mL of 5% phenol solution, then 5 mL of concentrated sulfuric acid was added and allowed to stand for 10 minutes. The mixture was uniformly mixed by vibration, and the absorbance at 490 nm of each set was measured using a spectrophotometer, and then the concentration was calculated by a standard curve. The results are recorded in Table 2.

  Based on the test results described above, each set of Chinese herbal extracts contains a large number of triterpenoids and polysaccharides, of which the content of Group A3 is the highest.

(B) Survival effects on uterine sarcoma cells In this study, human uterine sarcoma cells (MES-SA cells) were selectively used and purchased from the Food Industry Research Institute (BCRC 60333). Sarcoma cells are cultured in a culture solution containing 90% McCoy's 5a of 1.5 mM L-Glutamine and 10% fetal bovine serum (FBS). It was left in a culture box with a concentration of 5% and a humidity of 95% and replaced with a fresh culture medium once every two days.

  At the time of subculture, the uterine sarcoma cells are first centrifuged at 1000 rpm for 5 minutes, and the supernatant is removed. Then, a new culture is added again, and the number of cells in a 10 cm Petri dish is increased. Maintain about 1 × 10 5 to 1 × 10 6 cells / mL.

  When conducting this test, take out a 10 cm Petri dish containing the above-mentioned uterine sarcoma cells that have grown to 80-90%, remove the discolored culture medium, and wash the cells once with 8 mL of PBS buffer. Add added trypsin / ethylenediaminetetraacetic acid (Trypsin / EDTA) and react for 1 to 3 minutes, then gently shake the Petri dish to detach the cells from the wall, add the re-warmed medium, and gently lighten the cells. The culture solution containing cells was poured into a centrifuge tube and centrifuged at 1500 rpm for 10 minutes. After removing the supernatant of the separation solution, the medium containing serum is added again to disperse the cells uniformly. Further, 20 μL of cells are taken out, and 20 μL of 0.04% Trypan Blue is added. The cells were stained, and then a cell counter was inserted and the number of viable cells was counted with a microscope. Subsequent tests can be performed if the viability of the cells does not fall below 85%.

  Adjust the concentration of the uterine sarcoma cells to 1 × 10 5 cells / mL with a culture medium containing serum, and take 100 μL of each of the uterine sarcoma cells in a 96-well flat plate. 1 × 10 4 cells were further contained, and the cells were further cultured overnight in a culture box having a temperature of 37 ° C. and a carbon dioxide concentration of 5%.

  After 24 hours of culture, 100 μL of Kampo herb extract (dissolved in DMSO at a concentration of 2 mg / mL) shown in Table 3 was added and mixed uniformly. Then, the culture was continued overnight for 24 hours in a culture box with a carbon dioxide concentration of 5%.

  After 24 hours, the culture medium was removed by moving, rinsed with phosphate buffer, fresh culture medium containing 100 μL of CCK-8 was added again, the temperature was 37 ° C., and the concentration of carbon dioxide was 5 % In a culture box, reacted for 2 hours, shaken for 5 minutes, shaken for 5 minutes, all holes detected absorbance at a wavelength of 450 nm, and results of absorbance of control group not treated with any drug The survival rate was calculated based on The results are shown in Table 3.

  Referring to Table 3, based on the results of the above-described test, the herbal extract has the ability to suppress the growth of uterine sarcoma cells, and the A3 herbal extract is the best. Has efficacy. Therefore, the subsequent test was continued using the extract of the above-mentioned A3 set of herbal medicines.

(C) Effect of Otsuka on uterine sarcoma In this study, Wistar female Oki purchased from the National Laboratory Animal Center of the National Institute for Experimental Studies was selectively used. At the age of the week, the weight is between 180 grams and 190 grams. Daegu is kept in the animal center of Onishi University of Technology, and the room temperature is maintained between 20 ° C and 22 ° C. Lighting time and dark time are 12 hours each, and Daegu is free to eat It was raised in an environment where it was possible to drink water.

  Each through the gastric tube, RO water (C0 group) or A3 group Chinese herbal extract (C1 group) is taken from Daegu (the dose to be administered is 300 mg Chinese medicine in 1 kg every day) Herb extract) and at the same time, estrogen (dosage is 200 micrograms / dose) by intraperitoneal injection, and record daily body weight, food intake and water consumption of each group Four weeks later, blood was collected from the abdominal artery, the contents of estrogen and progesterone in the blood were measured, the uterus was removed, and tissue sections and H & E staining (hematoxylin-eosin) were performed. The results are as shown in Table 4 and FIGS.

  Referring to Table 4, there was no clear difference in body weight, food intake, and water intake after 28 days of each group of large bowels in this study, and the first group of large bowls administered the above-mentioned herbal extract. The estrogen and lutein hormone content in the blood is clearly reduced (the inhibition rate against estrogen reaches 47.5 ± 4.8% and the inhibition rate against lutein hormone is 33.1 ± 5.1%) Therefore, it means that the extract of herbal medicine has the ability to suppress the content of estrogen and lutein hormone in the body.

  FIG. 1 shows the result of H & E staining of the uterine section of the C0 group Captain, and FIG. 2 shows the H & E staining result of the uterine horn section of the Cap 0 set. Referring to FIGS. 1 and 2, the uterine smooth muscle cells are hypertrophy, have eosin-favorable cytoplasm, the cell boundaries are relatively unclear, and the arrangements are confused and confused. And the phenomenon of cell mitosis could not be observed, indicating that the fibroids in the C0th group were successfully produced.

  FIG. 3 shows the result of H & E staining of the uterine section of the C1 set of captains, and FIG. 4 shows the result of H & E staining of the uterine horn section of the group C1 captain. Referring to FIGS. 3 and 4, it is possible to know the result of staining of the tissue section of the C1 set of large coral, and when the above-mentioned herbal medicine extract is taken orally, it suppresses the increase of uterine smooth muscle and hypertrophy. It shows that the extract of the herbal medicine can successfully improve the activity of uterine smooth muscle growth and hypertrophy phenomenon.

  Taken together, the herbal medicinal herb composition for anti-uterine myoma of the present invention can keep the content of estrogen and lutein hormone in the body of the customer low by the composition distribution of cowpox, jaundice and beneficial mother grass, The effect that growth can be suppressed can be achieved.

  In addition, according to the extract of herbal medicine used to produce the use of the anti-uterine fibroid drug of the present invention, active ingredients from cowpox, jaundice and beneficial herb are extracted to effectively suppress the growth of uterine fibroids. As a result, the patient can be exempted from diseases such as menstrual pain and abnormal bleeding due to the growth of uterine fibroids, and can achieve the effect of improving the quality of life of the patient.

  The present invention may be implemented in other ways without departing from the spirit and essential characteristics thereof. Accordingly, the preferred embodiments described herein are exemplary and not intended to be limiting.

The present invention relates to Chinese medicinal herbs composition, in particular, relates to a traditional Chinese medicine herbal composition for anti uterine fibroids, further, the present invention is herbal herbal composition used to manufacture the anti-uterine fibroids drug It relates to the extract.

Moreover, Chinese medicine grass extract used to manufacture the by anti uterine fibroids drug present invention, the herbal medicine grass extract was prepared by a method comprising the following steps, herbal medicine with any of the composition of the A step of providing a herbal composition, a step of extracting the Chinese herb compound in 95 hours at 50 ° C. to 80 ° C. with 95% alcohol, and concentrating the extracted product, thereby Obtaining a herbal extract, wherein the weight-volume ratio of the herbal medicinal herb compound and the 95% alcohol is 1: 2, the beef bowl is a fruit body, the jaundice is a root, and the beneficial mother grass is It is a ground part.

Moreover, Chinese medicine grass extract used to manufacture the anti-uterine fibroids medicament according to the present invention, the herbal medicine grass extract by administering to one of consumers, the Disease fibroid the consumer The amount of the herbal extract that is prevented or improved and the herbal extract is administered to the consumer is 300 milligrams of the herbal extract per kilogram of the consumer's body weight every day, and for 28 consecutive days. It can also be administered.

According to the present invention, an extract of Chinese herbal medicine used to produce an anti-uterine fibroid drug extracts active ingredients from cowpox, jaundice and beneficial herb, and effectively inhibits the growth of uterine fibroids, There is an advantage that the patient can be exempted from diseases such as menstrual pain and abnormal bleeding due to the growth of uterine fibroids, and can achieve the effect of improving the quality of life of the patient.

In addition, according to the extract of Chinese herb used to produce the anti-uterine fibroid drug of the present invention, by extracting active ingredients from cowpox, jaundice and beneficial herb, and effectively suppressing the growth of uterine fibroids The patient can be exempt from diseases such as menstrual pain and abnormal bleeding due to the growth of uterine fibroids, and can achieve the effect of further improving the patient's quality of life.

Claims (7)

  Chinese herbal medicine for anti-uterine fibroids comprising 25% -75% beef meal, 20.8% -62.5% jaundice and 4.2% -12.5% beneficial mother grass in mass percentage object.   The herbal medicine composition for anti-uterine fibroids according to claim 1, wherein the herbal medicine composition contains 75% of cowpox in mass percentage.   The herbal medicine composition for anti-uterine fibroids according to claim 1, wherein the herbal medicine composition contains 20.8% jaundice in mass percentage.   The herbal medicine composition for anti-uterine fibroids according to claim 1, wherein the herbal medicine composition contains 4.2% of beneficial mother grass by mass percentage.   The herbal medicine composition for anti-uterine fibroids according to claim 1, wherein the herbal medicine composition contains 75% beef meal, 20.8% jaundice, and 4.2% beneficial mother grass by mass percentage. . An extract of a herbal medicinal herb used to produce a use of an anti-uterine myoma drug,
Providing a herbal medicine composition according to any one of claims 1 to 5,
Extracting the Chinese herbal herb compound in 8-12 hours with 95% alcohol at 50-80 ° C;
Obtaining the extract of the herbal medicine by concentrating the product of the extraction,
The anti-uterine fibroid drug characterized in that the weight-volume ratio of the above-mentioned herbal medicine compound and 95% sake is 1: 2, the beef bowl is the fruit body, the jaundice is the root, and the beneficial herb is the ground part. Extract of herbal medicine used to produce   The Kampo herbal extract is administered to one consumer to prevent or improve the uterine fibroid disease of the consumer, and the dosage of the Kampo herbal extract administered to the consumer is the demand. 7. The method according to claim 6, wherein 300 milligrams of the herbal medicine extract per kilogram of a person is administered daily and continuously for 28 days. Chinese herbal extract extracted.